TY  - JOUR
A1  - Hans, Stefan
A1  - Scheer, Nico
A1  - Riedl, Iris
A1  - Weizäcker, Elisabeth von
A1  - Blader, Patrick
A1  - Campos-Ortega, José A.
T1  - her3, a zebrafish member of the hairy-E(spl) family, is repressed by Notch signalling
JF  - Development
Y1  - 2004
U6  - https://doi.org/10.1242/dev.01167
SN  - 1477-9129
VL  - 131
IS  - 12
SP  - 2957
EP  - 2969
ER  - 
TY  - GEN
A1  - Sieker, T.
A1  - Tippkötter, Nils
A1  - Muffler, K.
A1  - Ulber, Roland
T1  - Herstellung von Ethanol, Phenolsäuren, organischen Säuren und Biogas durch vollständige Nutzung von Grassilage in einer Bioraffinerie
T2  - Chemie Ingenieur Technik
N2  - Gräser sind in der Lage, einen großen Teil der für eine biobasierte Wirtschaft benötigten Biomasse zur Verfügung zustellen. Um eine ganzjährige Nutzung des Grases zu gewährleisten, muss eine stabile Lagerung des Grases erreicht werden, was z. B. durch Silieren möglich ist. Die konservierende Wirkung der Silierung beruht auf der Bildung organischer Säuren. Um diese zu gewinnen, wird die Silage gepresst, die organischen Säuren über Flüssig/Flüssig-Extraktion aus dem Presssaft abgetrenntund mittels chromatographischer Methoden weiter aufgereinigt. Im präsentierten Konzept werden die im Presskuchen enthaltenen Lignocellulosen hydrolysiert und die erhaltenen Monosaccharide zu Ethanol fermentiert. Die Phenolsäuren, die in Gräsern die Rolle des Lignins übernehmen, können simultan mit der Hydrolyse der Polysaccharide enzymatisch abgetrennt und als Nebenprodukt gewonnen werden. Die nach der Abtrennung des Ethanols verbleibenden Fermentationsreststoffe werden für die Herstellung von Biogas verwendet.
Y1  - 2012
U6  - https://doi.org/10.1002/cite.201250415
SN  - 0009-286X
SN  - 1522-2640 (eISSN)
N1  - ProcessNet-Jahrestagung 2012 und 30. DECHEMA-Jahrestagung der Biotechnologen, 10. – 13. September 2012, Karlsruhe
N1  - Die präsentierte Arbeit wird vom BMELV über die FNR gefördert (Förder-kennzeichen 22025407).
VL  - 84
IS  - 8
SP  - 1297
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Kotter, Michael
A1  - Hammon, Ulrich
T1  - Herstellung von Formkörpern mit definierter Porenstruktur / Ulrich Hammon ; Michael Kotter
JF  - Chemie - Ingenieur - Technik. 56 (1984), H. 6
Y1  - 1984
SN  - 0009-286X
N1  - ISSN der E-Ausg.: 1522-2640
SP  - 455
EP  - 463
ER  - 
TY  - CHAP
A1  - Sieker, T.
A1  - Duwe, A.
A1  - Poth, S.
A1  - Tippkötter, Nils
A1  - Ulber, Roland
T1  - Herstellung von Itaconsäure aus Buchenholzhydrolysaten
T2  - Kurzfassungsband / GVC-DECHEMA Vortrags- und Diskussionstagung Biopharmazeutische Produktion : 14. - 16. Mai 2012. Konzerthaus Freibung
Y1  - 2012
SP  - 57
PB  - DECHEMA
CY  - Frankfurt, M.
ER  - 
TY  - JOUR
A1  - Kotter, Michael
T1  - Herstellung von Tränkkatalysatoren als verfahrenstechnische Aufgabe
JF  - Chemie - Ingenieur - Technik. 55 (1983), H. 3
Y1  - 1983
SN  - 0009-286X
N1  - ISSN der E-Ausg.: 1522-2640
SP  - 179
EP  - 185
ER  - 
TY  - JOUR
A1  - Kotter, Michael
A1  - Kirch, M.
A1  - Riekert, L.
T1  - Herstellung von Tränkkatalysatoren mit Nickel als Aktivkomponente auf porösen Trägern / R. Kirch ; M. Kotter ; L. Riekert
JF  - Chemiker-Zeitung. 108 (1984), H. 4
Y1  - 1984
SN  - 0009-2894
N1  - In der Bibliothek vorhanden: 63 ZS 024-1984 (Bereichsbibliothek Jülich)
SP  - 121
EP  - 129
ER  - 
TY  - JOUR
A1  - Prielmeier, Franz
A1  - Speedy, R. J.
A1  - Lüdemann, H.-D.
T1  - High Pressure NMR Self-Diffusion Studies on Supercooled Water
JF  - High Pressure Science and Technology Proceeding XI AIRAPT, Kiew. 1
Y1  - 1987
N1  - AIRAPT Conference / International Association for the Advancement of High Pressure Science and Technology
SP  - 75
ER  - 
TY  - JOUR
A1  - Prielmeier, Franz
A1  - Lang, E. W.
A1  - Radkowitsch, H.
A1  - Lüdemann, H. D.
T1  - High Pressure NMR Study of the Molecular Dynamics of Liquid fluoroform and deutero-fluoroform / Lang, E. W. ; Prielmeier, F. X. ; Radkowitsch, H. ; Lüdemann, H. D.
JF  - Berichte der Bunsen-Gesellschaft für Physikalische Chemie. 91 (1987), H. 10
Y1  - 1987
SN  - 0005-9021
SP  - 1025
EP  - 1033
ER  - 
TY  - JOUR
A1  - Prielmeier, Franz
A1  - Lang, E. W.
A1  - Radkowitsch, H.
A1  - Lüdemann, H.-D.
T1  - High Pressure NMR Study of the Molecular Dynamics of Liquid methyl fluoride and deutero-methyl fluoride / Lang, E. W. ; Prielmeier, F. X. ; Radkowitsch, H. ; Lüdemann, H. D.
JF  - Berichte der Bunsen-Gesellschaft für Physikalische Chemie. 91 (1987), H. 10
Y1  - 1987
SN  - 0005-9021
SP  - 1017
EP  - 1025
ER  - 
TY  - JOUR
A1  - Heine, A.
A1  - Herrmann, G.
A1  - Selmer, Thorsten
A1  - Terwesten, F.
A1  - Buckel, W.
A1  - Reuter, K.
T1  - High resolution crystal structure of clostridium propionicum β-Alanyl-CoA:Ammonia Lyase, a new member of the "Hot Dog Fold" protein superfamily
JF  - Proteins
N2  - Clostridium propionicum is the only organism known to ferment β-alanine, a constituent of coenzyme A (CoA) and the phosphopantetheinyl prosthetic group of holo-acyl carrier protein. The first step in the fermentation is a CoA-transfer to β-alanine. Subsequently, the resulting β-alanyl-CoA is deaminated by the enzyme β-alanyl-CoA:ammonia lyase (Acl) to reversibly form ammonia and acrylyl-CoA. We have determined the crystal structure of Acl in its apo-form at a resolution of 0.97 Å as well as in complex with CoA at a resolution of 1.59 Å. The structures reveal that the enyzme belongs to a superfamily of proteins exhibiting a so called “hot dog fold” which is characterized by a five-stranded antiparallel β-sheet with a long α-helix packed against it. The functional unit of all “hot dog fold” proteins is a homodimer containing two equivalent substrate binding sites which are established by the dimer interface. In the case of Acl, three functional dimers combine to a homohexamer strongly resembling the homohexamer formed by YciA-like acyl-CoA thioesterases. Here, we propose an enzymatic mechanism based on the crystal structure of the Acl·CoA complex and molecular docking. Proteins 2014; 82:2041–2053. © 2014 Wiley Periodicals, Inc.
Y1  - 2014
U6  - https://doi.org/10.1002/prot.24557
SN  - 1097-0134 (E-Journal); 0887-3585 (Print)
VL  - 82
IS  - 9
SP  - 2041
EP  - 2053
PB  - Wiley-Liss
CY  - New York
ER  - 
TY  - JOUR
A1  - Voigt, Birgit
A1  - Albrecht, Dirk
A1  - Sievers, Susanne
A1  - Becher, Dörte
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Schweder, Thomas
A1  - Maurer, Karl-Heinz
A1  - Hecker, Michael
T1  - High-resolution proteome maps of Bacillus licheniformis cells growing in minimal medium
JF  - Proteomics
Y1  - 2015
U6  - https://doi.org/10.1002/pmic.201400504
SN  - 1615-9861
VL  - 15
IS  - 15
SP  - 2629
EP  - 2633
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - GEN
A1  - Tippkötter, Nils
A1  - Maurer, S.
A1  - Pasteur, A.
A1  - Kampeis, P.
A1  - Ulber, Roland
T1  - Hochgradient-Magnetseparation von Fermentationsprodukten–FEM Simulation der Filtermatrix
T2  - Chemie Ingenieur Technik
N2  - Durch den Einsatz magnetisierbarer Partikel lassen sich Stoffwechselprodukte direkt und selektiv aus feststoffreichen Fermentationssuspensionen abtrennen. Im Gegensatz zu klassischen Adsorbermaterialien können magnetisierbare Partikel mit sehr geringen Durchmessern verwendet werden. Zur deren Abtrennung ist jedoch ein hoher Magnetfeldgradient notwendig. Dieser wird in der Regel durch in der Trennkammer bzw. dem Magnetfeld eingebrachte magnetisierbare Drähte realisiert. Bei der Auslegung der Drahtgitter ist ein Kompromiss zwischen Abtrennrate und Durchlässigkeit nötig. Die Ausrichtung der Drähte in Relation zum Magnetfeld, deren Abstand sowie die geometrische Anordnung können hierbei variiert werden. Zum Verständnis der Einflüsse auf das sich ausbildende Magnetfeld und die Fluiddynamik wurden Simulationen mit der Finite-Elemente-Methode durchgeführt und experimentell überprüft. Hierfür wurden die Drähte unter Variation von Anzahl, Richtung und Anordnung in den Hochgradient-Magnetseparator eingebracht. Erste Verifizierungen der Simulationen zeigen, dass die in Magnetfeldrichtung ausgerichteten Drähte (x-Achse) über die geringste Partikelrückhaltefähigkeit verfügen. Die Drähte der y- und z-Achse halten den größten Anteil der Magnetpartikel zurück, wobei die Drähte in y-Richtung den höchsten Feldgradienten ausbilden. Des Weiteren konnte gezeigt werden, dass eine rhomboedrische Drahtanordnung der kubischen vorzuziehen ist.
Y1  - 2010
U6  - https://doi.org/10.1002/cite.201050217
SN  - 0009-286X
SN  - 1522-2640 (eISSN)
N1  - ProcessNet-Jahrestagung 2010 und 28. DECHEMA-Jahrestagung der Biotechnologen, 21. - 23. September 2010, Eurogress Aachen
VL  - 82
IS  - 9
SP  - 1361
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - GEN
A1  - Duwe, A.
A1  - Tippkötter, Nils
A1  - Leipold, D.
A1  - Riemer, S.
A1  - Zorn, H.
A1  - Ulber, Roland
T1  - Holzhydrolyse als Feststoffreaktion: Charakterisierung von Inhibitoren und Erhöhung der Ausbeute durch den Einsatz lignolytischer Enzyme
T2  - Chemie Ingenieur Technik
N2  - Der Erhalt möglichst hoher Zuckerkonzentrationen für nachfolgende Fermentationen und eine Steigerung der Produktivität sind Ziele der Hydrolyse bei hohen Feststoffkonzentrationen im Rahmen des Projekts „Lignocellulose Bioraffinerie“. Verwendet wird durch ein Organosolv-Verfahren aufgeschlossenes Buchenholz. Die Hydrolyse des Faserstoffes erfolgt mithilfe von CTec2-Enzymen (Fa. Novozymes). Zurzeit können unter Einsatz eines neuen Feststoffreaktors Cellulosefasern in einer Konzentration bis 400 g L⁻¹ enzymatisch hydrolysiert werden. Dabei werden Ausbeuten (g Glucose/g Cellulose im Faserstoff) bis 0,86 g g⁻¹ und Glucosekonzentrationenvon 120 g L⁻¹ erreicht. Ein Nachteil ist jedoch die hierbei auftretende Abnahme der Hydrolyseausbeuten. Zahlreiche Limitierungen bezüglich der Hydrolysierbarkeit von Lignocellulose werden zurzeit diskutiert und publiziert. Ziel der Untersuchungen ist die Identifizierung hydrolysehemmender Substanzen sowie die Erhöhung der Ausbeute an Zuckermonomeren durch den Einsatz lignolytischer Enzyme. Hierbei wird eine HPLC-MS-Methode zur Charakterisierung hemmender Substanzen eingesetzt, um potenzielle Inhibitoren zu erfassen.
Y1  - 2012
U6  - https://doi.org/10.1002/cite.201250298
SN  - 0009-286X
SN  - 1522-2640 (eISSN)
N1  - ProcessNet-Jahrestagung 2012 und 30. DECHEMA-Jahrestagung der Biotechnologen, 10. – 13. September 2012, Karlsruhe
VL  - 84
IS  - 8
SP  - 1307
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - PAT
A1  - Jeromin, Günter Erich
A1  - Dietrich, Wolf
A1  - Giebeler, Eberhard
A1  - Weiss, Wolfgang
T1  - Holzschutzmittel : Europäische Patentschrift EP0293556B1 ; Veröffentlichungstag im Patentblatt: 23.01.1992 / Anmelder: Rütgerswerke AG, 6000 Frankfurt. Erfinder: Wolf Dietrich ; Eberhard Giebeler ; Günter Jeromin ; Wolfgang Weiss
Y1  - 1992
N1  - Volltext über Datenbank: http://publikationen.dpma.de/
PB  - Deutsches Patent- und Markenamt
CY  - München
ER  - 
TY  - JOUR
A1  - Ross, Jillian
A1  - Plummer, Simon M.
A1  - Rode, Anja
A1  - Scheer, Nico
A1  - Bower, Conrad C.
A1  - Vogel, Ortwin
A1  - Henderson, Colin J.
A1  - Wolf, C. Roland
A1  - Elcombe, Clifford R.
T1  - Human constitutive androstane receptor (CAR) and pregnane X receptor (PXR) support the  hypertrophic but not the hyperplastic response to the murine nongenotoxic hepatocarcinogens phenobarbital and chlordane in vivo
JF  - Toxicological Sciences
N2  - Mouse nongenotoxic hepatocarcinogens phenobarbital (PB) and chlordane induce hepatomegaly characterized by hypertrophy and hyperplasia. Increased cell proliferation is implicated in the mechanism of tumor induction. The relevance of these tumors to human health is unclear. The xenoreceptors, constitutive androstane receptors (CARs), and pregnane X receptor (PXR) play key roles in these processes. Novel “humanized” and knockout models for both receptors were developed to investigate potential species differences in hepatomegaly. The effects of PB (80 mg/kg/4 days) and chlordane (10 mg/kg/4 days) were investigated in double humanized PXR and CAR (huPXR/huCAR), double knockout PXR and CAR (PXRKO/CARKO), and wild-type (WT) C57BL/6J mice. In WT mice, both compounds caused increased liver weight, hepatocellular hypertrophy, and cell proliferation. Both compounds caused alterations to a number of cell cycle genes consistent with induction of cell proliferation in WT mice. However, these gene expression changes did not occur in PXRKO/CARKO or huPXR/huCAR mice. Liver hypertrophy without hyperplasia was demonstrated in the huPXR/huCAR animals in response to both compounds. Induction of the CAR and PXR target genes, Cyp2b10 and Cyp3a11, was observed in both WT and huPXR/huCAR mouse lines following treatment with PB or chlordane. In the PXRKO/CARKO mice, neither liver growth nor induction of Cyp2b10 and Cyp3a11 was seen following PB or chlordane treatment, indicating that these effects are CAR/PXR dependent. These data suggest that the human receptors are able to support the chemically induced hypertrophic responses but not the hyperplastic (cell proliferation) responses. At this time, we cannot be certain that hCAR and hPXR when expressed in the mouse can function exactly as the genes do when they are expressed in human cells. However, all parameters investigated to date suggest that much of their functionality is maintained.
Y1  - 2010
U6  - https://doi.org/10.1093/toxsci/kfq118
SN  - 1096-0929
VL  - 116
IS  - 2
SP  - 452
EP  - 466
PB  - Oxford University Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Whitehead, Mark
A1  - Öhlschläger, Peter
A1  - Almajhdi, Fahad N.
A1  - Alloza, Leonor
A1  - Marzábal, Pablo
A1  - Meyers, Ann E.
A1  - Hitzeroth, Inga I.
A1  - Rybicki, Edward P.
T1  - Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice
JF  - BMC cancer
Y1  - 2014
U6  - https://doi.org/10.1186/1471-2407-14-367
SN  - 1471-2407
IS  - 14:367
SP  - 1
EP  - 15
PB  - BioMed Central
CY  - London
ER  - 
TY  - JOUR
A1  - Öhlschläger, Peter
A1  - Osen, Wolfram
A1  - Dell, Kerstin
A1  - Faath, Stefan
T1  - Human papillomavirus type 16 L1 capsomeres induce L1-specific cytotoxic T lymphocytes and tumor regression in C57BL/6 mice / Öhlschläger, Peter ; Osen, Wolfram ; Dell, Kerstin ; Faath, Stefan ; Garcea Robert L: ; Jochmus, Ingrid ; Müller, Martin, Pawlita,
JF  - Journal of Virology. 77 (2003), H. 8
Y1  - 2003
SN  - 1098-5514
SP  - 4635
EP  - 4645
ER  - 
TY  - JOUR
A1  - Danho, Waleed
A1  - Naithani, Vinod K.
A1  - Sasaki, André N.
A1  - Föhles, Joseph
A1  - Berndt, Heinz
A1  - Büllesbach, Erika E.
A1  - Zahn, H.
T1  - Human proinsulin, VII : synthesis of two protected peptides corresponding to the sequences 1—45 and 46—86 of the prohormone
JF  - Hoppe-Seyler's Zeitschrift für physiologische Chemie
Y1  - 1980
U6  - https://doi.org/10.1515/bchm2.1980.361.1.857
SN  - 1437-4315
SN  - 0018-4888
VL  - 361
IS  - 1
SP  - 857
EP  - 863
ER  - 
TY  - CHAP
A1  - Kazuki, Yasuhiro
A1  - Kobayashi, Kaoru
A1  - Hirabayashi, Masumi
A1  - Abe, Satoshi
A1  - Kajitani, Naoyo
A1  - Kazuki, Kanoko
A1  - Takehara, Shoko
A1  - Takiguchi, Masato
A1  - Satoh, Daisuke
A1  - Kuze, Jiro
A1  - Sakuma, Tetsushi
A1  - Kaneko, Takehito
A1  - Mashimo, Tomoji
A1  - Osamura, Minori
A1  - Hashimoto, Mari
A1  - Wakatsuki, Riko
A1  - Hirashima, Rika
A1  - Fujiwara, Ryoichi
A1  - Deguchi, Tsuneo
A1  - Kurihara, Atsushi
A1  - Tsukazaki, Yasuko
A1  - Senda, Naoto
A1  - Yamamoto, Takashi
A1  - Scheer, Nico
A1  - Oshimura, Mitsuo
T1  - Humanized UGT2 and CYP3A transchromosomic rats for improved prediction of human drug metabolism
T2  - PNAS Proceedings of the National Academy of Sciences of the United States of America
Y1  - 2019
U6  - https://doi.org/10.1073/pnas.1808255116
SN  - 1091-6490
VL  - 116
IS  - 8
SP  - 3072
EP  - 3081
ER  - 
TY  - JOUR
A1  - Breuer, Lars
A1  - Raue, Markus
A1  - Strobel, M.
A1  - Mang, Thomas
A1  - Schöning, Michael Josef
A1  - Thoelen, R.
A1  - Wagner, Torsten
T1  - Hydrogels with incorporated graphene oxide as light-addressable actuator materials for cell culture environments in lab-on-chip systems
JF  - Physica status solidi (a)
N2  - Abstractauthoren Graphene oxide (GO) nanoparticles were incorporated in temperature-sensitive Poly(N-isopropylacrylamide) (PNIPAAm) hydrogels. The nanoparticles increase the light absorption and convert light energy into heat efficiently. Thus, the hydrogels with GO can be stimulated spatially resolved by illumination as it was demonstrated by IR thermography. The temporal progression of the temperature maximum was detected for different concentrations of GO within the polymer network. Furthermore, the compatibility of PNIPAAm hydrogels with GO and cell cultures was investigated. For this purpose, culture medium was incubated with hydrogels containing GO and the viability and morphology of chinese hamster ovary (CHO) cells was examined after several days of culturing in presence of this medium.
Y1  - 2016
U6  - https://doi.org/10.1002/pssa.201533056
SN  - 1862-6300
VL  - 213
IS  - 6
SP  - 1520
EP  - 1525
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Tix, Julian
A1  - Moll, Fabian
A1  - Krafft, Simone
A1  - Betsch, Matthias
A1  - Tippkötter, Nils
T1  - Hydrogen production from enzymatic pretreated organic waste with thermotoga neapolitana
JF  - Energies
N2  - Biomass from various types of organic waste was tested for possible use in hydrogen production. The composition consisted of lignified samples, green waste, and kitchen scraps such as fruit and vegetable peels and leftover food. For this purpose, the enzymatic pretreatment of organic waste with a combination of five different hydrolytic enzymes (cellulase, amylase, glucoamylase, pectinase and xylase) was investigated to determine its ability to produce hydrogen (H2) with the hydrolyzate produced here. In course, the anaerobic rod-shaped bacterium T. neapolitana was used for H2 production. First, the enzymes were investigated using different substrates in preliminary experiments. Subsequently, hydrolyses were carried out using different types of organic waste. In the hydrolysis carried out here for 48 h, an increase in glucose concentration of 481% was measured for waste loads containing starch, corresponding to a glucose concentration at the end of hydrolysis of 7.5 g·L−1. In the subsequent set fermentation in serum bottles, a H2 yield of 1.26 mmol H2 was obtained in the overhead space when Terrific Broth Medium with glucose and yeast extract (TBGY medium) was used. When hydrolyzed organic waste was used, even a H2 yield of 1.37 mmol could be achieved in the overhead space. In addition, a dedicated reactor system for the anaerobic fermentation of T. neapolitana to produce H2 was developed. The bioreactor developed here can ferment anaerobically with a very low loss of produced gas. Here, after 24 h, a hydrogen concentration of 83% could be measured in the overhead space.
KW  - Biological hydrogen
KW  - Organic waste
KW  - Dark fermentation
KW  - Hydrolysis
KW  - Pretreatment
Y1  - 2024
U6  - https://doi.org/10.3390/en17122938
SN  - 1996-1073
N1  - Corresponding author: Nils Tippkötter
VL  - 17
IS  - 12
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Lempiäinen, Harri
A1  - Couttet, Philippe
A1  - Bolognani, Federico
A1  - Müller, Arne
A1  - Dubost, Valérie
A1  - Luisier, Raphaëlle
A1  - Rio-Espinola, Alberto del
A1  - Vitry, Veronique
A1  - Unterberger, Elif B.
A1  - Thomson, John P.
A1  - Treindl, Fridolin
A1  - Metzger, Ute
A1  - Wrzodek, Clemens
A1  - Hahne, Florian
A1  - Zollinger, Tulipan
A1  - Brasa, Sarah
A1  - Kalteis, Magdalena
A1  - Marcellin, Magali
A1  - Giudicelli, Fanny
A1  - Braeuning, Albert
A1  - Morawiec, Laurent
A1  - Zamurovic, Natasa
A1  - Längle, Ulrich
A1  - Scheer, Nico
A1  - Schübeler, Dirk
A1  - Goodman, Jay
A1  - Chibout, Salah-Dine
A1  - Marlowe, Jennifer
A1  - Theil, Dietlinde
A1  - Heard, David J.
A1  - Grenet, Olivier
A1  - Zell, Andreas
A1  - Templin, Markus F.
A1  - Meehan, Richard R.
A1  - Wolf, Roland C.
A1  - Elcombe, Clifford R.
A1  - Schwarz, Michael
A1  - Moulin, Pierre
A1  - Terranova, Rémi
A1  - Moggs, Jonathan G.
T1  - Identification of Dlk1-Dio3 imprinted gene cluster non-coding RNAs as novel candidate biomarkers for liver tumor promotion
JF  - Toxicological Sciences
N2  - The molecular events during nongenotoxic carcinogenesis and their temporal order are poorly understood but thought to include long-lasting perturbations of gene expression. Here, we have investigated the temporal sequence of molecular and pathological perturbations at early stages of phenobarbital (PB) mediated liver tumor promotion in vivo. Molecular profiling (mRNA, microRNA [miRNA], DNA methylation, and proteins) of mouse liver during 13 weeks of PB treatment revealed progressive increases in hepatic expression of long noncoding RNAs and miRNAs originating from the Dlk1-Dio3 imprinted gene cluster, a locus that has recently been associated with stem cell pluripotency in mice and various neoplasms in humans. PB induction of the Dlk1-Dio3 cluster noncoding RNA (ncRNA) Meg3 was localized to glutamine synthetase-positive hypertrophic perivenous hepatocytes, sug- gesting a role for β-catenin signaling in the dysregulation of Dlk1-Dio3 ncRNAs. The carcinogenic relevance of Dlk1-Dio3 locus ncRNA induction was further supported by in vivo genetic dependence on constitutive androstane receptor and β-catenin pathways. Our data identify Dlk1-Dio3 ncRNAs as novel candidate early biomarkers for mouse liver tumor promotion and provide new opportunities for assessing the carcinogenic potential of novel compounds.
Y1  - 2012
U6  - https://doi.org/10.1093/toxsci/kfs303
SN  - 1094-2025
VL  - 131
IS  - 2
SP  - 375
EP  - 386
PB  - Oxford University Press
CY  - Oxford
ER  - 
TY  - CHAP
A1  - Kalbe, Jochen
A1  - Kuropka, Rolf
A1  - Meyer-Stork, L. Sebastian
A1  - Lauter, S. L.
A1  - Höcker, Hartwig
A1  - Berndt, Heinz
ED  - Körner, Andrea
T1  - Identification of fine animal hair via DNA analysis
T2  - Proceedings of the 1st International Symposium on Specialty Animal Fibers : Aachen, October 26 - 27, 1987 ; [scientific, technological and economical aspects] . - (Schriftenreihe des Deutschen Wollforschungsinstitutes an der Technischen Hochschule Aachen e.V. ; 103)
Y1  - 1988
SN  - 0930-3723
SP  - 221
EP  - 227
PB  - Dt. Wollforschungsinst.
CY  - Aachen
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
T1  - Identification of marker peptides in hair for discrimination with regard to species and breed / Koerner, Andrea ; Ring, Maike ; Fabry, Marlies ; Elbers, Gereon ; Moeller, Martin
JF  - Proceedings of the Aachen-Dresden International Textile Conference, 3rd, Aachen, Germany, Nov. 26-27, 2009
Y1  - 2009
N1  - Aachen-Dresden International Textile Conference ; (3 : ; 2009.11.26-27 : ; Aachen)
PB  - DWI
CY  - Aachen
ER  - 
TY  - BOOK
A1  - Schmich, Peter
T1  - Identifizierung und Bestimmung von freien Radikalen bei der Kohlenwasserstoff-Pyrolyse : Experimente und Computersimulationen / [vorgelegt von Peter Schmich]
Y1  - 1988
N1  - Heidelberg, Univ., Diss., 1988.
CY  - Heidelberg
ER  - 
TY  - BOOK
A1  - Jeromin, Günter Erich
A1  - Mang, Thomas
T1  - Immobilisierte Enzyme auf magnetisierbaren Trägern / Offenlegungsschrift: DE102004005198A1 ; Offenlegungstag: 25.08.2005
Y1  - 2005
N1  - Volltext über Datenbank DEPATISnet
PB  - Deutsches Marken- und Patentamt
CY  - München
ER  - 
TY  - BOOK
A1  - Lassonczyk, Beate
T1  - Immobilisierung löslicher organischer Stoffe aus Auflagehorizonten in Sandböden
Y1  - 1987
N1  - Bonn, Univ., Diss., 1987
CY  - Bonn
ER  - 
TY  - PAT
A1  - Jeromin, Günter Erich
T1  - Immobilisierung von Alkoholdehydrogenasen und deren Coenzyme sowie Verwendung des Immobilisats : Offenlegungsschrift : DE 102008038326 A1 Offenlegungstag: 25.02.2010
Y1  - 2010
PB  - Deutsches Patent- und Markenamt
CY  - München
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Lüllau, E.
A1  - Dreisbach, C.
A1  - Grogg, A.
T1  - Immobilization of animal cells on chemically modified siran carrier / Lüllau, E. ; Dreisbach, C. ; Grogg, A. ; Biselli, M. ; Wandrey, C.
JF  - Animal cell technology : developments, processes, and products ; ESACT, European Society for Animal Cell Technology, the 11th meeting / Ed. R. E. Spier
Y1  - 1992
SN  - 0750604212
SP  - 469
EP  - 475
PB  - Butterworth-Heinemann
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Kotter, Michael
A1  - Riekert, L.
T1  - Impregnation-type catalysts with nonuniform distribution of the active component
JF  - Chemical engineering fundamentals. 2 (1983)
Y1  - 1983
SN  - 0723-0966
SP  - 19
EP  - 38
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Noll, Thomas
A1  - Eisenkrätzer, Detlef
A1  - Kiesewetter, André
T1  - Improved product formation in high density Chinese hamster ovary cell cultures transfected at confluency / Thomas Noll, Detlef Eisenkrätzer, André Kiesewetter, André Dinter, Steffen Zeng, Christian Wandrey, Manfred Biselli und Eric G. Berger
JF  - Biotechnology Letters. 24 (2002), H. 11
Y1  - 2002
SN  - 0141-5492
SP  - 861
EP  - 866
ER  - 
TY  - JOUR
A1  - Lowis, Carsten
A1  - Ferguson, Simon
A1  - Paulßen, Elisabeth
A1  - Hoehr, Cornelia
T1  - Improved Sc-44 production in a siphon-style liquid target on a medical cyclotron
JF  - Applied Radiation and Isotopes
Y1  - 2021
U6  - https://doi.org/10.1016/j.apradiso.2021.109675
SN  - 0969-8043
VL  - 172
IS  - Art. 109675
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Scheer, Nico
A1  - Ross, Jillian
A1  - Kapelyukh, Yury
A1  - Rode, Anja
A1  - Wolf, C. Roland
T1  - In vivo responses of the human and murine pregnane X receptor to dexamethasone in mice
JF  - Drug Metabolism and Disposition
N2  - Dexamethasone (DEX) is a potent and widely used anti-inflammatory and immunosuppressant glucocorticoid. It can bind and activate the pregnane X receptor (PXR), which plays a critical role as xenobiotic sensor in mammals to induce the expression of many enzymes, including cytochromes P450 in the CYP3A family. This induction results in its own metabolism. We have used a series of transgenic mouse lines, including a novel, improved humanized PXR line, to compare the induction profile of PXR-regulated drug-metabolizing enzymes after DEX administration, as well as looking at hepatic responses to rifampicin (RIF). The new humanized PXR model has uncovered further intriguing differences between the human and mouse receptors in that RIF only induced Cyp2b10 in the new humanized model. DEX was found to be a much more potent inducer of Cyp3a proteins in wild-type mice than in mice humanized for PXR. To assess whether PXR is involved in the detoxification of DEX in the liver, we analyzed the consequences of high doses of the glucocorticoid on hepatotoxicity on different PXR genetic backgrounds. We also studied these effects in an additional mouse model in which functional mouse Cyp3a genes have been deleted. These strains exhibited different sensitivities to DEX, indicating a protective role of the PXR and CYP3A proteins against the hepatotoxicity of this compound.
Y1  - 2010
U6  - https://doi.org/10.1124/dmd.109.031872
SN  - 1521-009X
VL  - 38
IS  - 7
SP  - 1046
EP  - 1053
PB  - ASPET
CY  - Bethesda
ER  - 
TY  - JOUR
A1  - Teumer, T.
A1  - Capitain, C.
A1  - Ross-Jones, J.
A1  - Tippkötter, Nils
A1  - Rädle, M.
A1  - Methner, F.-J.
T1  - In-line Haze Monitoring Using a Spectrally Resolved Back Scattering Sensor
JF  - BrewingScience
N2  - In the present work an optical sensor in combination with a spectrally resolved detection device for in-line particle-size-monitoring for quality control in beer production is presented. The principle relies on the size and wavelength dependent backscatter of growing particles in fluids. Measured interference structures of backscattered light are compared with calculated theoretical values, based on Mie-Theory, and fitted with a linear least square method to obtain particle size distributions. For this purpose, a broadband light source in combination with a process-CCD-spectrometer (charge ? coupled device spectrometer) and process adapted fiber optics are used. The goal is the development of an easy and flexible measurement device for in-line-monitoring of particle size. The presented device can be directly installed in product fill tubes or vessels, follows CIP- (cleaning in place) and removes the need of sample taking. A proof of concept and preliminary results, measuring protein precipitation, are presented.
Y1  - 2018
SN  - 1613-2041
VL  - 71
IS  - 5/6
SP  - 49
EP  - 55
PB  - Fachverlag Hans Carl
CY  - Nürnberg
ER  - 
TY  - JOUR
A1  - Engel, Mareike
A1  - Holtmann, Dirk
A1  - Ulber, Roland
A1  - Tippkötter, Nils
T1  - Increased Biobutanol Production by Mediator‐Less Electro‐Fermentation
JF  - Biotechnology Journal
N2  - A future bio-economy should not only be based on renewable raw materials but also in the raise of carbon yields of existing production routes. Microbial electrochemical technologies are gaining increased attention for this purpose. In this study, the electro-fermentative production of biobutanol with C. acetobutylicum without the use of exogenous mediators is investigated regarding the medium composition and the reactor design. It is shown that the use of an optimized synthetic culture medium allows higher product concentrations, increased biofilm formation, and higher conductivities compared to a synthetic medium supplemented with yeast extract. Moreover, the optimization of the reactor system results in a doubling of the maximum product concentrations for fermentation products. When a working electrode is polarized at −600 mV vs. Ag/AgCl, a shift from butyrate to acetone and butanol production is induced. This leads to an increased final solvent yield of Yᴀᴃᴇ = 0.202 gg⁻¹ (control 0.103 gg⁻¹), which is also reflected in a higher carbon efficiency of 37.6% compared to 23.3% (control) as well as a fourfold decrease in simplified E-factor to 0.43. The results are promising for further development of biobutanol production in bioelectrochemical systems in order to fulfil the principles of Green Chemistry.
Y1  - 2018
U6  - https://doi.org/10.1002/biot.201800514
SN  - 1860-7314
VL  - 14
IS  - 4
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Martinez, Ronny
A1  - Jakob, Felix
A1  - Tu, Ran
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
A1  - Schwaneberg, Ulrich
T1  - Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution
JF  - Biotechnology and bioengineering
Y1  - 2013
SN  - 1097-0290 (E-Journal); 0006-3592 (Print); 0368-1467 (Print)
VL  - Vol. 110
IS  - Iss. 3
SP  - 711
EP  - 720
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Herold, Marco J.
A1  - Brandt, Jens van den
A1  - Seibler, Jost
A1  - Reichardt, Holger M.
T1  - Inducible and reversible gene silencing by stable integration of an shRNA-encoding lentivirus in transgenic rats
JF  - Proceedings of the National Academy of Sciences of the United States of America
Y1  - 2008
U6  - https://doi.org/10.1073/pnas.0806213105
SN  - 1091-6490
VL  - 105
IS  - 47
SP  - 18507
EP  - 18512
ER  - 
TY  - JOUR
A1  - Kotnik, Katarina
A1  - Popova, Elena
A1  - Todiras, Mihail
A1  - Mori, Marcelo A.
A1  - Alenina, Natalia
A1  - Seibler, Jost
A1  - Bader, Michael
T1  - Inducible transgenic rat model for diabetes mellitus based on shRNA-mediated gene knockdown
JF  - Plos One
Y1  - 2009
U6  - https://doi.org/10.1371/journal.pone.0005124
VL  - 4
IS  - 4
SP  - e5124
PB  - Plos
CY  - San Francisco, California, US
ER  - 
TY  - JOUR
A1  - Paulßen, Elisabeth
A1  - Le, Van So
A1  - Lengkeek, Nigel
A1  - Pellegrini, Paul
A1  - Jackson, Tim
A1  - Greguric, Ivan
A1  - Weiner, Ron
T1  - Influence of Metal Ions on the 68Ga-labeling of DOTATATE
JF  - Applied Radiation and Isotopes
Y1  - 2013
U6  - https://doi.org/10.1016/j.apradiso.2013.08.010
SN  - 1872-9800
VL  - 82
SP  - 232
EP  - 238
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - El Bergui, Omnia
A1  - Abouabdillah, Aziz
A1  - Bourioug, Mohamed
A1  - Schmitz, Dominik
A1  - Biel, Markus
A1  - Aboudrare, Abdellah
A1  - Krauss, Manuel
A1  - Jomaa, Ahlem
A1  - Romuli, Sebastian
A1  - Müller, Joachim
A1  - Fagroud, Mustapha
A1  - Bouabid, Rachid
T1  - Innovative solutions for drought: Evaluating hydrogel application on onion cultivation (Allium cepa) in Morocco
JF  - Water
N2  - Throughout the last decade, and particularly in 2022, water scarcity has become a critical concern in Morocco and other Mediterranean countries. The lack of rainfall during spring was worsened by a succession of heat waves during the summer. To address this drought, innovative solutions, including the use of new technologies such as hydrogels, will be essential to transform agriculture. This paper presents the findings of a study that evaluated the impact of hydrogel application on onion (Allium cepa) cultivation in Meknes, Morocco. The treatments investigated in this study comprised two different types of hydrogel-based soil additives (Arbovit® polyacrylate and Huminsorb® polyacrylate), applied at two rates (30 and 20 kg/ha), and irrigated at two levels of water supply (100% and 50% of daily crop evapotranspiration; ETc). Two control treatments were included, without hydrogel application and with both water amounts. The experiment was conducted in an open field using a completely randomized design. The results indicated a significant impact of both hydrogel-type dose and water dose on onion plant growth, as evidenced by various vegetation parameters. Among the hydrogels tested, Huminsorb® Polyacrylate produced the most favorable outcomes, with treatment T9 (100%, HP, 30 kg/ha) yielding 70.55 t/ha; this represented an increase of 11 t/ha as compared to the 100% ETc treatment without hydrogel application. Moreover, the combination of hydrogel application with 50% ETc water stress showed promising results, with treatment T4 (HP, 30 kg, 50%) producing almost the same yield as the 100% ETc treatment without hydrogel while saving 208 mm of water.
KW  - water economy
KW  - yield
KW  - deficit irrigation
KW  - hydrogel
KW  - onion
Y1  - 2023
U6  - https://doi.org/10.3390/w15111972
VL  - 15
IS  - 11
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Ulber, Roland
A1  - Tippkötter, Nils
A1  - Buchholz, H.
A1  - Demmer, W.
A1  - Scheper, T.
T1  - Innovative Verfahren in der Molkeaufarbeitung zur Gewinnung neuer Produkte
JF  - Deutsche Milchwirtschaft
Y1  - 2008
SN  - 0012-0480
VL  - 59
IS  - 19
SP  - 704
EP  - 706
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Thömmes, J.
A1  - Garske, U.
A1  - Wandrey, Christian
T1  - Integrated detoxification: Reduction of ammonium concentration by dialysis with cation exchange membranes / Thömmes, J. ; Garske, U. ; Biselli, M. ; Wandrey, C.
JF  - Animal cell technology : developments, processes, and products ; ESACT, European Society for Animal Cell Technology, the 11th meeting / Ed. R. E. Spier
Y1  - 1992
SN  - 0750604212
SP  - 171
EP  - 175
PB  - Butterworth-Heinemann
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Schiedermeier, Maximilian
A1  - Rettner, Cornelius
A1  - Heilmann, Marcel
A1  - Schneider, Felix
A1  - Marz, Martin
T1  - Interference of automotive HV-DC-systems by traction voltage-source-inverters (VSI)
JF  - 2019 IEEE Transportation Electrification Conference (ITEC-India)
Y1  - 2019
U6  - https://doi.org/10.1109/ITEC-India48457.2019.ITECINDIA2019-37
SP  - 1
EP  - 6
PB  - IEEE
CY  - New York
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Behner, T.
A1  - Remme, S.
A1  - Prissok, F.
T1  - Interstitial Metal Impurities as a Possible Cause of Enhanced Reactivities / T. Behner, G. Elbers, S. Remme, F. Prissok, P. Stegger, and G. Lehmann
JF  - Berichte der Bunsen-Gesellschaft : physical chemistry, chemical physics. 90 (1986)
Y1  - 1986
SN  - 0005-9021
SP  - 698
ER  - 
TY  - CHAP
A1  - Ulber, Roland
A1  - Muffler, Kai
A1  - Tippkötter, Nils
A1  - Hirth, Thomas
A1  - Sell, Dieter
ED  - Ulber, Roland
ED  - Sell, Dieter
ED  - Hirth, Thomas
T1  - Introduction to Renewable Resources in the Chemical Industry
T2  - Renewable raw materials : new feedstocks for the chemical industry
Y1  - 2011
SN  - 978-3-527-32548-1
SP  - 1
EP  - 6
PB  - Wiley-VCH-Verlag
CY  - Weinheim
ET  - 1. Auflage
ER  - 
TY  - JOUR
A1  - Capitain, Charlotte
A1  - Wagner, Sebastian
A1  - Hummel, Joana
A1  - Tippkötter, Nils
T1  - Investigation of C–N Formation Between Catechols and Chitosan for the Formation of a Strong, Novel Adhesive Mimicking Mussel Adhesion
JF  - Waste and Biomass Valorization
Y1  - 2021
U6  - https://doi.org/10.1007/s12649-020-01110-5
SN  - 1877-265X
N1  - Corresponding author: Nils Tippkötter
VL  - 12
SP  - 1761
EP  - 1779
PB  - Springer Nature
CY  - Cham
ER  - 
TY  - JOUR
A1  - Mang, Thomas
A1  - Kolz, J.
A1  - Martins, J.
A1  - Kremer, K.
T1  - Investigation of Elastomer Foams by Single-Sided NMR / Kolz, J. ; Martins, J. ; Kremer, K. ; Blümich, B. ; Mang, Thomas
JF  - Kurzfassungen : 3. bis 6. Juli 2006, Nürnberg = Summaries / Deutsche Kautschuk Tagung, 3.7.-6.7.2006
Y1  - 2006
N1  - Deutsche Kautschuk-Tagung ; <2006, Nürnberg >
SP  - 33
PB  - DKG, Dt. Kautschuk-Ges.
CY  - Frankfurt, Main
ER  - 
TY  - JOUR
A1  - Raue, Markus
A1  - Wambach, M.
A1  - Glöggler, S.
A1  - Grefen, Dana
A1  - Kaufmann, R.
A1  - Abetz, C.
A1  - Georgopanos, P.
A1  - Handge, U. A.
A1  - Mang, Thomas
A1  - Blümich, B.
A1  - Abetz, V.
T1  - Investigation of historical hard rubber ornaments of Charles Goodyear
JF  - Macromolecular chemistry and physics
Y1  - 2014
SN  - 1022-1352
VL  - Vol. 215
IS  - No. 3
SP  - 245
EP  - 254
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Mang, Thomas
A1  - Kolz, J.
A1  - Hunger, K.
A1  - Rath, S.
T1  - Investigation of self-diffusion processes in ionic hydrogels with a single-sided sensor / Kolz, J. ; Hunger, K. ; Rath, S. ; Mang, T. ;  Blümich, B.
JF  - Magnetic Resonance Imaging. 25 (2007), H. 4
Y1  - 2007
SN  - 0730-725X
N1  - Proceedings of the Eighth International Bologna Conference on Magnetic Resonance in Porous Media
SP  - 566
EP  - 567
ER  - 
TY  - JOUR
A1  - Mang, Thomas
A1  - Kolz, J.
A1  - Martins, J.
A1  - Kremer, K.
T1  - Investigation of the Elastomer-Foam Production with Single-Sided NMR / Kolz, J. ; Martins, J. ; Kremer, K. ; Mang, Thomas ; Blümich, B.
JF  - Kautschuk Gummi Kunststoffe. 60 (2007), H. 4
Y1  - 2007
SN  - 0948-3276
SP  - 179
EP  - 183
ER  - 
TY  - JOUR
A1  - Breuer, Lars
A1  - Mang, Thomas
A1  - Schöning, Michael Josef
A1  - Thoelen, Ronald
A1  - Wagner, Torsten
T1  - Investigation of the spatial resolution of a laser-based stimulation process for light-addressable hydrogels with incorporated graphene oxide by means of IR thermography
JF  - Sensors and Actuators A: Physical
Y1  - 2017
U6  - https://doi.org/10.1016/j.sna.2017.11.031
SN  - 0924-4247
VL  - 268
SP  - 126
EP  - 132
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Christoph, Thomas
A1  - Bahrenberg, Gregor
A1  - Vry, Jean de
A1  - Englberger, Werner
A1  - Erdmann, Volker A.
A1  - Frech, Moritz
A1  - Kögel, Babette
A1  - Röhl, Thomas
A1  - Schiene, Klaus
A1  - Schröder, Wolfgang
A1  - Seibler, Jost
A1  - Kurreck, Jens
T1  - Investigation of TRPV1 loss-of-function phenotypes in transgenic shRNA expressing and knockout mice
JF  - Molecular and Cellular Neuroscience
Y1  - 2008
U6  - https://doi.org/10.1016/j.mcn.2007.12.006
SN  - 1044-7431
VL  - 37
IS  - 3
SP  - 579
EP  - 589
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Klose, W.
T1  - Investigations into the Mechanism of the Deactivation of a Palladium-Supported Catalyst by Hydrogen Sulfide Poisoning. Klose, W. ; Feuerriegel, U.
JF  - Chemical engineering & technology. 21 (1998), H. 7
Y1  - 1998
SN  - 0930-7516
N1  - gleicher Artikel in Deutsch In: Chem.Ing.Tech 70(1998) No  S. 449-452 u.d.T.: Untersuchungen zum Mechanismus der Desaktivierung eines Palladium-Trägerkatalysators durch Vergiftung mit Schwefelwasserstoff
SP  - 562
EP  - 564
ER  - 
TY  - JOUR
A1  - Burmistrova, Natalia A.
A1  - Soboleva, Polina M.
A1  - Monakhova, Yulia
T1  - Is infrared spectroscopy combined with multivariate analysis a promising tool for heparin authentication?
JF  - Journal of Pharmaceutical and Biomedical Analysis
N2  - The investigation of the possibility to determine various characteristics of powder heparin (n = 115) was carried out with infrared spectroscopy. The evaluation of heparin samples included several parameters such as purity grade, distributing company, animal source as well as heparin species (i.e. Na-heparin, Ca-heparin, and heparinoids). Multivariate analysis using principal component analysis (PCA), soft independent modelling of class analogy (SIMCA), and partial least squares – discriminant analysis (PLS-DA) were applied for the modelling of spectral data. Different pre-processing methods were applied to IR spectral data; multiplicative scatter correction (MSC) was chosen as the most relevant.

Obtained results were confirmed by nuclear magnetic resonance (NMR) spectroscopy. Good predictive ability of this approach demonstrates the potential of IR spectroscopy and chemometrics for screening of heparin quality. This approach, however, is designed as a screening tool and is not considered as a replacement for either of the methods required by USP and FDA.
KW  - IR spectroscopy
KW  - Heparin
KW  - Authenticity
KW  - Principal component analysis
KW  - Soft independent modeling of class analogy
Y1  - 2021
SN  - 0731-7085
U6  - https://doi.org/10.1016/j.jpba.2020.113811
VL  - 194
IS  - Article number: 113811
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Burger, René
A1  - Rumpf, Jessica
A1  - Do, Xuan Tung
A1  - Monakhova, Yulia
A1  - Diehl, Bernd W. K.
A1  - Rehahn, Matthias
A1  - Schulze, Margit
T1  - Is NMR combined with multivariate regression applicable for the molecular weight determination of randomly cross-linked polymers such as lignin?
JF  - ACS Omega
N2  - The molecular weight properties of lignins are one of the key elements that need to be analyzed for a successful industrial application of these promising biopolymers. In this study, the use of 1H NMR as well as diffusion-ordered spectroscopy (DOSY NMR), combined with multivariate regression methods, was investigated for the determination of the molecular weight (Mw and Mn) and the polydispersity of organosolv lignins (n = 53, Miscanthus x giganteus, Paulownia tomentosa, and Silphium perfoliatum). The suitability of the models was demonstrated by cross validation (CV) as well as by an independent validation set of samples from different biomass origins (beech wood and wheat straw). CV errors of ca. 7–9 and 14–16% were achieved for all parameters with the models from the 1H NMR spectra and the DOSY NMR data, respectively. The prediction errors for the validation samples were in a similar range for the partial least squares model from the 1H NMR data and for a multiple linear regression using the DOSY NMR data. The results indicate the usefulness of NMR measurements combined with multivariate regression methods as a potential alternative to more time-consuming methods such as gel permeation chromatography.
Y1  - 2021
U6  - https://doi.org/10.1021/acsomega.1c03574
SN  - 2470-1343
VL  - 6
IS  - 44
SP  - 29516
EP  - 29524
PB  - ACS Publications
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Lindner, Simon
A1  - Burger, René
A1  - Rutledge, Douglas N.
A1  - Do, Xuan Tung
A1  - Rumpf, Jessica
A1  - Diehl, Bernd W. K.
A1  - Schulze, Margit
A1  - Monakhova, Yulia
T1  - Is the calibration transfer of multivariate calibration models between high- and low-field NMR instruments possible? A case study of lignin molecular weight
JF  - Analytical chemistry
N2  - Although several successful applications of benchtop nuclear magnetic resonance (NMR) spectroscopy in quantitative mixture analysis exist, the possibility of calibration transfer remains mostly unexplored, especially between high- and low-field NMR. This study investigates for the first time the calibration transfer of partial least squares regressions [weight average molecular weight (Mw) of lignin] between high-field (600 MHz) NMR and benchtop NMR devices (43 and 60 MHz). For the transfer, piecewise direct standardization, calibration transfer based on canonical correlation analysis, and transfer via the extreme learning machine auto-encoder method are employed. Despite the immense resolution difference between high-field and low-field NMR instruments, the results demonstrate that the calibration transfer from high- to low-field is feasible in the case of a physical property, namely, the molecular weight, achieving validation errors close to the original calibration (down to only 1.2 times higher root mean square errors). These results introduce new perspectives for applications of benchtop NMR, in which existing calibrations from expensive high-field instruments can be transferred to cheaper benchtop instruments to economize.
Y1  - 2022
SN  - 1520-6882
U6  - https://doi.org/10.1021/acs.analchem.1c05125
VL  - 94
IS  - 9
SP  - 3997
EP  - 4004
PB  - ACS Publications
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Kalbe, Jochen
A1  - Kuropka, Rolf
A1  - Meyer-Stork, L. Sebastian
A1  - Berndt, Heinz
A1  - Sauter, Sybille L.
A1  - Loss, Peter
A1  - Hendo, Karsten
A1  - Riesner, Detlev
A1  - Höcker, Hartwig
T1  - Isolation and characterization of high-molecular mass DNA from hair shafts
JF  - Biological chemistry
Y1  - 1988
SN  - 0177-3593
U6  - https://doi.org/10.1515/bchm3.1988.369.1.413
VL  - 369
IS  - 1
SP  - 413
EP  - 416
ER  - 
TY  - GEN
A1  - Sieker, T.
A1  - Duwe, A.
A1  - Poth, S.
A1  - Tippkötter, Nils
A1  - Ulber, Roland
T1  - Itaconsäureherstellung aus Buchenholz-Hydrolysaten
T2  - Chemie Ingenieur Technik
N2  - Aus hölzernen Cellulosen und Hemicellulosen können durch enzymatische Hydrolyse fermentierbare Zucker für die Herstellung von Chemikalien und Treibstoffen gewonnen werden. Die bisherige Forschung fokussiert sich oft auf die Nutzung dieser Zucker zur Gewinnung von Ethanol. Daneben muss aber auch die stoffliche Nutzung zur Gewinnung von Grundchemikalien berücksichtigt werden. Eine solche Grundchemikalie ist Itakonsäure. Obwohl die biotechnologische Itaconsäureproduktion bereits eingehend untersucht und etabliert ist, gestaltet sie sich im Rahmen von Bioraffinerien der zweiten Generation als schwierig, da der überwiegend verwendete Produktionsorganismus gegen eine weite Bandbreite von Inhibitoren sensibel ist. Die Herstellung von Itaconsäure aus Buchenholzhydrolysaten wird im Rahmen der deutschen Lignocellulose-Bioraffinerie entwickelt. Die unbehandelten Hydrolysate ermöglichen weder das Wachstum von Aspergillus terreus noch die Bildung von Itaconsäure. Daher werden Möglichkeiten zur Konditionierung des Hydrolysates mit dem Ziel einer Itaconsäureproduktion mit hohen Ausbeuten und Konzentrationen vorgestellt.
Y1  - 2012
U6  - https://doi.org/10.1002/cite.201250414
SN  - 0009-286X
SN  - 1522-2640 (eISSN)
N1  - ProcessNet-Jahrestagung 2012 und 30. DECHEMA-Jahrestagung der Biotechnologen, 10. – 13. September 2012, Karlsruhe
N1  - Die präsentierte Arbeit wird vomBMELV über die FNR gefördert (Förderkennzeichen 22019409).
VL  - 84
IS  - 8
SP  - 1300
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - BOOK
A1  - Lauth, Jakob
A1  - Hagemeyer, Alfred
A1  - Lautensack, Thomas
A1  - Deimling, Axel
T1  - Katalysator und Verfahren für die katalytische oxidative Dehydrierung von Alkylaromaten = Catalyst and process for the catalytically oxidative dyhydrogenation of alkyl aromatics : Europäische Patentschrift EP0768996B1 ; EP-Patenterteilungsdatum: 12.05.1999 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Alfred Hagemeyer ; Günter Lauth ; Thomas Lautensack ; Axel Deimling
Y1  - 1999
N1  - Volltext über Datenbank: http://publikationen.dpma.de/ ; Internationale Veröffentlichungsnummer: WO 96/01796
PB  - Europäisches Patentamt
CY  - [München u.a.]
ER  - 
TY  - PAT
A1  - Lauth, Jakob
A1  - Hagemeyer, Alfred
A1  - Lautensack, Thomas
A1  - Deimling, Axel
T1  - Katalysator und Verfahren für die katalytische oxidative Dehydrierung von Alkylaromaten und Paraffinen : Offenlegungsschrift DE4423975A1 ; Veröffentlichungstag im Patentblatt : 11.01.1996 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Alfred Hagemeyer ; Günter Lauth ; Thomas Lautensack ; Axel Deimling
Y1  - 1996
N1  - Volltext über Datenbank: http://publikationen.dpma.de/
PB  - Deutsches Patent- und Markenamt
CY  - München
ER  - 
TY  - PAT
A1  - Lauth, Jakob
A1  - Hagemeyer, Alfred
A1  - Lautensack, Thomas
A1  - Deimling, Axel
T1  - Katalysator und Verfahren für die katalytische oxidative Dehydrierung von Alkylaromaten und Paraffinen = Catalyst and process for the catalytically oxidative dyhydrogenation of alkyl aromatics and paraffins : Internationale [Patent]anmeldung WO 96/001796 ; Veröffentlichungsdatum: 25.01.1996  / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Alfred Hagemeyer ; Günter Lauth ; Thomas Lautensack ; Axel Deimling
Y1  - 1996
N1  - Volltext über Datenbank http://depatisnet.dpma.de/
PB  - Weltorganisation für geistiges Eigentum, Internat. Büro
CY  - [Genf]
ER  - 
TY  - JOUR
A1  - Kotter, Michael
A1  - Riekert, L.
A1  - Weyland, F.
T1  - Katalytische Abgasreinigung an Nichtedelmetall-Trägerkontakten
JF  - Verfahrenstechnik  : VT ; Informationen für Planung, Bau und Betrieb von Apparaten und Anlagen. 17 (1983)
Y1  - 1983
SN  - 0049-5948
N1  - 2. ISSN: 0175-5315 , 3. ISSN: 0341-2644
SP  - 607
EP  - 615
ER  - 
TY  - JOUR
A1  - Kotter, Michael
T1  - Katalytische Abluftreinigung mit integriertem regenerativem Wärmetausch
JF  - Chemie - Ingenieur - Technik. 64 (1992), H. 9
Y1  - 1992
SN  - 0009-286X
N1  - ISSN der E-Ausg.: 1522-2640 ; GVC-Jahrentreffer ; Beitrag 116
SP  - 848
ER  - 
TY  - JOUR
A1  - Kotter, Michael
A1  - Lintz, Hans-Günther
A1  - Turek, Thomas
T1  - Katalytische Stickoxid-Reduktion in einem rotierenden Wärmeübertrager
JF  - Chemie - Ingenieur - Technik. 64 (1992), H. 5
Y1  - 1992
SN  - 0009-286X
N1  - ISSN der E-Ausg.: 1522-2640
SP  - 446
EP  - 448
ER  - 
TY  - CHAP
A1  - Scheer, Nico
A1  - Chu, Xiaoyan
A1  - Salphati, Laurent
A1  - Zamek-Gliszczynski, Maciej J.
ED  - Nicholls, Glynis
T1  - Knockout and humanized animal models to study membrane transporters in drug development
T2  - Drug Transporters: Volume 1: Role and Importance in ADME and Drug Development
Y1  - 2016
SN  - 978-1-78262-379-3
U6  - https://doi.org/10.1039/9781782623793-00298
SP  - 298
EP  - 332
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - PAT
A1  - Jeromin, Günter Erich
T1  - Kohlensäurediester, deren Herstellung und Verwendung als Riechstoffe und Aromastoffe : Offenlegungsschrift DE19802198A1 ; Offenlegungstag:  29.07.1999
Y1  - 1999
N1  - Volltext über Datenbank: http://publikationen.dpma.de/
PB  - Deutsches Patent- und Markenamt
CY  - München
ER  - 
TY  - GEN
A1  - Engel, M.
A1  - Bayer, H.
A1  - Ulber, Roland
A1  - Tippkötter, Nils
T1  - Kommt es in Elektrofermentationen mit Clostridium acetobutylicum zu einer Eisenlimitierung?
T2  - Chemie Ingenieur Technik
N2  - Das strikt anaerobe Bakterium Clostridium acetobutylicum bildet die Lösemittel Aceton, Butanol und Ethanol (ABE-Fermentation). Im Fall einer Eisenlimitierung kommt es zusätzlich zu einer Riboflavinsekretion (RF), was durch die gelbe Färbung des Kulturüberstands erkennbar ist. In dieser Arbeit wurde beobachtet, dass während Elektrofermentationen mit C. acetobutylicum bei –600 mV eine gelbe Färbung auftritt. Es wurde deshalb untersucht, ob eine Eisenlimitierung im bio-elektrochemischen System (BES) vorliegt. Hierzu wurden die Flavinspezien bei Kultivierungen in Medien mit einer Eisenlimitierung bzw. mit ausreichend Eisen in Serumflaschen sowie im BES mit und ohne angelegtem Potenzial verglichen. In den Serumflaschenversuchen wurden RF und Flavinadenindinukleotid (FAD)-Konzentrationen von ‡ 20 mg L⁻¹ sowie Flavinmononukleotid (FMN)-Konzentrationen von ca. 5 mg L⁻¹ detektiert. Bei ausreichender Eisenverfügbarkeit hingegen wurden in den Serumflaschen fast keine Flavine sekretiert. Im BES bei –600 mV hingegen wurde auch in diesen Kulturüberständen FMN und FAD (1–5 mg L⁻¹ ),jedoch kein RF gemessen. Diese Ergebnisse zeigen, dass die Flavinbildung im BES mit angelegtem Potenzial nicht mit der Flavinbildung unter Eisenlimitierung in Serumflaschen korreliert. Andere Faktoren für eine mögliche Flavinbildung werden aktuell näher betrachtet.
Y1  - 2018
U6  - https://doi.org/10.1002/cite.201855047
SN  - 0009-286X
SN  - 1522-2640 (eISSN)
N1  - ProcessNet-Jahrestagung und 33. DECHEMA-Jahrestagung der Biotechnologen 2018, 10. - 13. September 2018, Eurogress Aachen
VL  - 90
IS  - 9
SP  - 1154
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Hör, G.
T1  - Kompartimentmodelle zur Quantifizierung des myokardialen Stoffwechsels mit PET / U. W. Scherer, G. Hör
JF  - Kardiologische Nuklearmedizin / G. Hör [Hrsg.]
Y1  - 1997
SN  - 3-609-62970-3
SP  - 179
EP  - 189
PB  - ecomed
CY  - Landsberg
ER  - 
TY  - JOUR
A1  - Svaneborg, Carsten
A1  - Karimi-Varzaneh, Hossein Ali
A1  - Hojdis, Nils
A1  - Fleck, Franz
A1  - Everaers, Ralf
T1  - Kremer-Grest Models for Universal Properties of Specific Common Polymer Species
JF  - Soft Condensed Matter
N2  - The Kremer-Grest (KG) bead-spring model is a near standard in Molecular Dynamic simulations of generic polymer properties. It owes its popularity to its computational efficiency, rather than its ability to represent specific polymer species and conditions. Here we investigate how to adapt the model to match the universal properties of a wide range of chemical polymers species. For this purpose we vary a single parameter originally introduced by Faller and Müller-Plathe, the chain stiffness. Examples include polystyrene, polyethylene, polypropylene, cis-polyisoprene, polydimethylsiloxane, polyethyleneoxide and styrene-butadiene rubber. We do this by matching the number of Kuhn segments per chain and the number of Kuhn segments per cubic Kuhn volume for the polymer species and for the Kremer-Grest model. We also derive mapping relations for converting KG model units back to physical units, in particular we obtain the entanglement time for the KG model as function of stiffness allowing for a time mapping. To test these relations, we generate large equilibrated well entangled polymer melts, and measure the entanglement moduli using a static primitive-path analysis of the entangled melt structure as well as by simulations of step-strain deformation of the model melts. The obtained moduli for our model polymer melts are in good agreement with the experimentally expected moduli.
Y1  - 2018
IS  - 1606.05008
ER  - 
TY  - JOUR
A1  - Everaers, Ralf
A1  - Karimi-Varzaneh, Hossein Ali
A1  - Fleck, Franz
A1  - Hojdis, Nils
A1  - Svaneborg, Carsten
T1  - Kremer–Grest Models for Commodity Polymer Melts: Linking Theory, Experiment, and Simulation at the Kuhn Scale
JF  - Macromolecules
N2  - The Kremer–Grest (KG) polymer model is a standard model for studying generic polymer properties in molecular dynamics simulations. It owes its popularity to its simplicity and computational efficiency, rather than its ability to represent specific polymers species and conditions. Here we show that by tuning the chain stiffness it is possible to adapt the KG model to model melts of real polymers. In particular, we provide mapping relations from KG to SI units for a wide range of commodity polymers. The connection between the experimental and the KG melts is made at the Kuhn scale, i.e., at the crossover from the chemistry-specific small scale to the universal large scale behavior. We expect Kuhn scale-mapped KG models to faithfully represent universal properties dominated by the large scale conformational statistics and dynamics of flexible polymers. In particular, we observe very good agreement between entanglement moduli of our KG models and the experimental moduli of the target polymers.
Y1  - 2020
U6  - https://doi.org/10.1021/acs.macromol.9b02428
SN  - 1520-5835
VL  - 53
IS  - 6
SP  - 1901
EP  - 1916
PB  - ACS Publications
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Schnitzler, Thomas
T1  - Kultivierung der Hybridomazelllinie CF-10H5 (DSMZ ACC477)
JF  - Application notes / Sartorius stedim biotech
Y1  - 2009
PB  - Sartorius Stedim Biotec
CY  - Aubagne
ER  - 
TY  - PAT
A1  - Wieland, Susanne
A1  - Siegert, Petra
A1  - Spitz, Astrid
A1  - Maurer, Karl-Heinz
A1  - O'Connell, Timothy
A1  - Prüser, Inken
A1  - Schiedel, Marc-Steffen
A1  - Eiting, Thomas
A1  - Sendor-Müller, Dorota
A1  - Bastigkeit, Thorsten
A1  - Benda, Konstantin
A1  - Müller, Sven
T1  - Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Proteasen [Offenlegungsschrift]
T1  - Storage-stable liquid washing or cleaning agent containing proteases [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2011
SP  - 1
EP  - 25
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Kratz, J. V.
A1  - Schädel, M.
A1  - Brüchle, W.
T1  - Lawrencium Chemistry: No Evidence for Oxidation States Lower than 3+ in Aqueous Solution / U.W. Scherer, J.V. Kratz, M. Schädel, W. Brüchle, K.E. Gregorich, R.A. Henderson, D. Lee, M. Nurmia, D.C. Hoffman
JF  - Inorganica Chimica Acta. 146 (1988)
Y1  - 1988
SN  - 0020-1693
SP  - 249
EP  - 254
ER  - 
TY  - PAT
A1  - Bessler, Cornelius
A1  - Evers, Stefan
A1  - Maurer, Karl-Heinz
A1  - Merkel, Marion
A1  - Siegert, Petra
A1  - Weber, Angrit
A1  - Wieland, Susanne
T1  - Leistungsverbesserte Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen [Offenlegungsschrift]
T1  - Improved-performance proteases and detergents and cleaning agents comprising said proteases [Internationale Patentanmeldung]
Y1  - 2009
SP  - 1
EP  - 41
PB  - Deutsches Patent- und Markenamt / WIPO
CY  - München / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Schwaneberg, Ulrich
A1  - Martinez Moya, Ronny
A1  - Merkel, Marion
A1  - Spitz, Astrid
A1  - Wieland, Susanne
A1  - Hellmuth, Hendrik
A1  - Maurer, Karl-Heinz
T1  - Leistungsverbesserte Proteasevariante [Offenlegungsschrift]
T1  - Performance-enhanced protease variant [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2012
SP  - 1
EP  - 29
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - JOUR
A1  - Welden, Rene
A1  - Jablonski, Melanie
A1  - Wege, Christina
A1  - Keusgen, Michael
A1  - Wagner, Patrick Hermann
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
T1  - Light-Addressable Actuator-Sensor Platform for Monitoring and Manipulation of pH Gradients in Microfluidics: A Case Study with the Enzyme Penicillinase
JF  - Biosensors
N2  - The feasibility of light-addressed detection and manipulation of pH gradients inside an electrochemical microfluidic cell was studied. Local pH changes, induced by a light-addressable electrode (LAE), were detected using a light-addressable potentiometric sensor (LAPS) with different measurement modes representing an actuator-sensor system. Biosensor functionality was examined depending on locally induced pH gradients with the help of the model enzyme penicillinase, which had been immobilized in the microfluidic channel. The surface morphology of the LAE and enzyme-functionalized LAPS was studied by scanning electron microscopy. Furthermore, the penicillin sensitivity of the LAPS inside the microfluidic channel was determined with regard to the analyte’s pH influence on the enzymatic reaction rate. In a final experiment, the LAE-controlled pH inhibition of the enzyme activity was monitored by the LAPS.
KW  - microfluidics
KW  - enzyme kinetics
KW  - actuator-sensor system
KW  - light-addressable electrode
KW  - light-addressable potentiometric sensor
Y1  - 2021
U6  - https://doi.org/10.3390/bios11060171
SN  - 2079-6374
N1  - This article belongs to the Special Issue "Selected Papers from the 1st International Electronic Conference on Biosensors (IECB 2020)"
VL  - 11
IS  - 6
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Breuer, Lars
A1  - Raue, Markus
A1  - Kirschbaum, M.
A1  - Mang, Thomas
A1  - Schöning, Michael Josef
A1  - Thoelen, R.
A1  - Wagner, Torsten
T1  - Light-controllable polymeric material based on temperature-sensitive hydrogels with incorporated graphene oxide
JF  - Physica status solidi (a)
N2  - Poly(N-isopropylacrylamide) (PNIPAAm) hydrogel films with incorporated graphene oxide (GO) were developed and tested as light-stimulated actuators. GO dispersions were synthesized via Hummers method and characterized toward their optical properties and photothermal energy conversion. The hydrogels were prepared by means of photopolymerization. In addition, the influence of GO within the hydrogel network on the lower critical solution temperature (LCST) was investigated by differential scanning calorimetry (DSC). The optical absorbance and the response to illumination were determined as a function of GO concentration for thin hydrogel films. A proof of principle for the stimulation with light was performed.
Y1  - 2015
U6  - https://doi.org/10.1002/pssa.201431944
SN  - 1862-6319
VL  - 212
IS  - 6
SP  - 1368
EP  - 1374
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - CHAP
A1  - Breuer, Lars
A1  - Raue, Markus
A1  - Mang, Thomas
A1  - Schöning, Michael Josef
A1  - Thoelen, Ronald
A1  - Wagner, Torsten
T1  - Light-stimulated hydrogel actuators with incorporated graphene oxide for microfluidic applications
T2  - 12. Dresdner Sensor-Symposium 2015
Y1  - 2015
U6  - https://doi.org/10.5162/12dss2015/P5.8
SP  - 206
EP  - 209
ER  - 
TY  - JOUR
A1  - Bergs, Michel
A1  - Monakhova, Yulia
A1  - Diehl, Bernd W.
A1  - Konow, Christopher
A1  - Völkering, Georg
A1  - Pude, Ralf
A1  - Schulze, Margit
T1  - Lignins isolated via catalyst-free organosolv pulping from Miscanthus x giganteus, M. sinensis, M. robustus and M. nagara: a comparative study
JF  - Molecules
N2  - As a low-input crop, Miscanthus offers numerous advantages that, in addition to agricultural applications, permits its exploitation for energy, fuel, and material production. Depending on the Miscanthus genotype, season, and harvest time as well as plant component (leaf versus stem), correlations between structure and properties of the corresponding isolated lignins differ. Here, a comparative study is presented between lignins isolated from M. x giganteus, M. sinensis, M. robustus and M. nagara using a catalyst-free organosolv pulping process. The lignins from different plant constituents are also compared regarding their similarities and differences regarding monolignol ratio and important linkages. Results showed that the plant genotype has the weakest influence on monolignol content and interunit linkages. In contrast, structural differences are more significant among lignins of different harvest time and/or season. Analyses were performed using fast and simple methods such as nuclear magnetic resonance (NMR) spectroscopy. Data was assigned to four different linkages (A: β-O-4 linkage, B: phenylcoumaran, C: resinol, D: β-unsaturated ester). In conclusion, A content is particularly high in leaf-derived lignins at just under 70% and significantly lower in stem and mixture lignins at around 60% and almost 65%. The second most common linkage pattern is D in all isolated lignins, the proportion of which is also strongly dependent on the crop portion. Both stem and mixture lignins, have a relatively high share of approximately 20% or more (maximum is M. sinensis Sin2 with over 30%). In the leaf-derived lignins, the proportions are significantly lower on average. Stem samples should be chosen if the highest possible lignin content is desired, specifically from the M. x giganteus genotype, which revealed lignin contents up to 27%. Due to the better frost resistance and higher stem stability, M. nagara offers some advantages compared to M. x giganteus. Miscanthus crops are shown to be very attractive lignocellulose feedstock (LCF) for second generation biorefineries and lignin generation in Europe.
Y1  - 2021
U6  - https://doi.org/10.3390/molecules26040842
SN  - 1420-3049
N1  - Special Issue Lignin – A Natural Resource with Huge Potential https://www.mdpi.com/journal/molecules/special_issues/lignin_natural
VL  - 26
IS  - 4
PB  - MDPI
CY  - Basel
ER  - 
TY  - GEN
A1  - Poth, S.
A1  - Monzon, M.
A1  - Tippkötter, Nils
A1  - Ulber, Roland
T1  - Lignocellulose-Bioraffinerie: Simultane Verzuckerung und Fermentation
T2  - Chemie Ingenieur Technik
N2  - Die am häufigsten genutzten Rohstoffe für die Produktion von Treibstoffen und Chemikalien sind fossilen Ursprungs. Da diese limitiert sind, werden im Hinblick auf die Nachhaltigkeit alternative, erneuerbare Rohstoffquellen intensiv untersucht. Vielversprechend in diesem Kontext sind die in Lignocellulose enthaltenen Zucker, die beispielsweise zur Produktion von Ethanol genutzt werden können. In der Regel sind für eine Lig-nocellulose-Bioraffinerie mehrere Prozessschritte notwendig: Vorbehandlung, Verzuckerung und Fermentation. Um diesen Prozess einfacher zu gestalten, ist es möglich, die Verzuckerung und die Fermentation in einem Schritt durchzuführen (SSF). Als Substrat wird hier Cellulose-Faserstoff verwendet, der durch das Organosolv-Verfahren aufgeschlossen wurde. Die Hydrolyse erfolgt mit kommerziell erhältlichen Enzymen und für die Fermentation zu Ethanol werden zwei Hefen verwendet. Beim SSF-Verfahren konnte, im Vergleich zur entkoppelten Verfahrensweise, trotz bestehender Unterschiede in den Temperatur-Optima von Enzymen und Hefen eine Steigerung in der Ethanol-Ausbeute von 0,15 auf 0,2 gg⁻¹ beobachtet werden. Um wirtschaftliche Ausbeuten und Konzentrationen des Produkts erzielen zu können, ist es notwendig den Prozess weiter zu optimieren. Im Einzelfall muss überprüft werden, ob diese Verfahrensweise auch für die Produktion anderer interessanter Stoffe (wie Itaconsäure, Bernsteinsäure) geeignet ist.
KW  - Lignocellulose-Bioraffinerie
KW  - Prozessintegration
Y1  - 2010
U6  - https://doi.org/10.1002/cite.201050360
N1  - ProcessNet-Jahrestagung 2010 und 28. DECHEMA-Jahrestagung der Biotechnologen, 21. - 23. September 2010, Eurogress Aachen
VL  - 82
IS  - 9
SP  - 1568
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - CHAP
A1  - Duwe, A.
A1  - Tippkötter, Nils
A1  - Ulber, Roland
T1  - Lignocellulose-Biorefinery: Ethanol-Focused
T2  - Biorefineries
N2  - The development prospects of the world markets for petroleum and other liquid fuels are diverse and partly contradictory. However, comprehensive changes for the energy supply of the future are essential. Notwithstanding the fact that there are still very large deposits of energy resources from a geological point of view, the finite nature of conventional oil reserves is indisputable. To reduce our dependence on oil, the EU, the USA, and other major economic zones rely on energy diversification. For this purpose, alternative materials and technologies are being sought, and is most obvious in the transport sector. The objective is to progressively replace fossil fuels with renewable and more sustainable fuels. In this respect, biofuels have a pre-eminent position in terms of their capability of blending with fossil fuels and being usable in existing cars without substantial modification. Ethanol can be considered as the primary renewable liquid fuel. In this chapter enzymes, micro-organisms, and processes for ethanol production based on renewable resources are described.
KW  - Bioethanol
KW  - Biorefinery
KW  - Lignocellulose feedstook
Y1  - 2018
U6  - https://doi.org/10.1007/10_2016_72
N1  - Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE,volume 166)
SP  - 177
EP  - 215
PB  - Springer
CY  - Cham
ER  - 
TY  - CHAP
A1  - Poth, Sebastian
A1  - Monzon, Magaly
A1  - Tippkötter, Nils
A1  - Ulber, Roland
T1  - Lignocellulosic biorefinery : process integration of hydrolysis and fermentation
T2  - Proceedings / 11th European Workshop on Lignocellulosics and Pulp : August 16 - 19, 2010, Hamburg, Germany
Y1  - 2010
SP  - 65
EP  - 68
PB  - vTi
CY  - Hamburg
ER  - 
TY  - JOUR
A1  - Poth, Sebastian
A1  - Monzon, Magaly
A1  - Tippkötter, Nils
A1  - Ulber, Roland
T1  - Lignocellulosic biorefinery: Process integration of hydrolysis and fermentation (SSF process)
JF  - Holzforschung
N2  - The aim of the present work is the process integration and the optimization of the enzymatic hydrolysis of wood and the following fermentation of the products to ethanol. The substrate is a fiber fraction obtained by organosolv pre-treatment of beech wood. For the ethanol production, a co-fermentation by two different yeasts (Saccharomyces cerevisiae and Pachysolen tannophilus) was carried out to convert glucose as well as xylose. Two approaches has been followed: 1. A two step process, in which the hydrolysis of the fiber fraction and the fermentation to product are separated from each other. 2. A process, in which the hydrolysis and the fermentation are carried out in one single process step as simultaneous saccharification and fermentation (SSF). Following the first approach, a yield of about 0.15 g ethanol per gram substrate can be reached. Based on the SSF, one process step can be saved, and additionally, the gained yield can be raised up to 0.3 g ethanol per gram substrate.
Y1  - 2011
N1  - 11th EWLP, Hamburg, Germany, August 16–19, 2010
VL  - 65
IS  - 5
SP  - 633
EP  - 637
PB  - De Gruyter
CY  - Berlin
ER  - 
TY  - CHAP
A1  - Engel, Mareike
A1  - Thieringer, Julia
A1  - Tippkötter, Nils
T1  - Linking bioprocess engineering and electrochemistry for sustainable biofuel production
T2  - Young Researchers Symposium, YRS 2016. Proceedings
N2  - Electromicrobial engineering is an emerging, highly interdisciplinary research area linking bioprocesses with electrochemistry. In this work, microbial electrosynthesis (MES) of biobutanol is carried out during acetone-butanol-ethanol (ABE) fermentations with Clostridium acetobutylicum. A constant electric potential of −600mV (vs. Ag/AgCl) with simultaneous addition of the soluble redox mediator neutral red is used in order to study the electron transfer between the working electrode and the bacterial cells. The results show an earlier initiation of solvent production for all fermentations with applied potential compared to the conventional ABE fermentation. The f inal butanol concentration can be more than doubled by the application of a negative potential combined with addition of neutral red. Moreover a higher biofilm formation on the working electrode compared to control cultivations has been observed. In contrast to previous studies, our results also indicate that direct electron transfer (DET) might be possible with C. acetobutylicum. The presented results make microbial butanol production economically attractive and therefore support the development of sustainable production processes in the chemical industry aspired by the “Centre for resource-efficient chemistry and raw material change” as well as the the project “NanoKat” working on nanostructured catalysts in Kaiserslautern.
Y1  - 2016
N1  - Young Researchers Symposium, YRS 2016, 14th - 15th April 2016, Fraunhofer-Zentrum Kaiserslautern
SP  - 49
EP  - 53
PB  - Fraunhofer Verlag
CY  - Karlsruhe
ER  - 
TY  - PAT
A1  - Banowski, Bernhard
A1  - Wadle, Armin
A1  - Siegert, Petra
A1  - Sättler, Andrea
T1  - Lipase-Inhibitoren in Deodorantien und Antitranspirantien [Offenlegungsschrift]
T1  - Lipase inhibitors in deodorants and antiperspirants [Europäische Patentanmeldung]
Y1  - 2004
SP  - 1
EP  - 22
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt
CY  - München / Den Hague
ER  - 
TY  - JOUR
A1  - Prielmeier, Franz
A1  - Hörstermann, D.
A1  - Gyngell, M. L.
A1  - Merboldt, K.-D.
T1  - Localized Proton MRS of Acute and Chronic Gyperglycemia in Rat Brain in vivo / D. Hörstermann, F. Prielmeier , M. L. Gyngell, K.-D. Merboldt, W. Hänicke, J. Frahm
JF  - Book of Abstracts, SMRM, 11th Annual Meeting Berlin
Y1  - 1992
N1  - Society of Magnetic Resonance in Medicine
SP  - 2740
ER  - 
TY  - CHAP
A1  - Tippkötter, Nils
A1  - Möhring, Sophie
A1  - Roth, Jasmine
A1  - Wulfhorst, Helene
T1  - Logistics of lignocellulosic feedstocks: preprocessing as a preferable option
T2  - Biorefineries
N2  - In comparison to crude oil, biorefinery raw materials are challenging in concerns of transport and storage. The plant raw materials are more voluminous, so that shredding and compacting usually are necessary before transport. These mechanical processes can have a negative influence on the subsequent biotechnological processing and shelf life of the raw materials. Various approaches and their effects on renewable raw materials are shown. In addition, aspects of decentralized pretreatment steps are discussed. Another important aspect of pretreatment is the varying composition of the raw materials depending on the growth conditions. This problem can be solved with advanced on-site spectrometric analysis of the material.
KW  - Analytics
KW  - Decentral
KW  - Mechanical
KW  - On-site
KW  - Pre-treatment
Y1  - 2019
SN  - 978-3-319-97117-9
SN  - 978-3-319-97119-3
U6  - https://doi.org/10.1007/10_2017_58
N1  - Advances in biochemical engineering/biotechnology ; Vol. 166
SP  - 43
EP  - 68
PB  - Springer
CY  - Cham
ER  - 
TY  - JOUR
A1  - Meiners, Klaus
A1  - Baumann, Marcus
T1  - Lokale Beiträge zum globalen Klimaschutz
JF  - Wechselwirkung : Wissenschaft & vernetztes Denken
Y1  - 1997
SN  - 0172-1623
VL  - 19
IS  - 84
SP  - 6
EP  - 12
ER  - 
TY  - RPRT
A1  - Tippkötter, Nils
T1  - Lokale Vorbehandlung nachwachsender Rohstoffe für Bioraffinerien (BioSats) : Schlussbericht zum Vorhaben : Laufzeit: 01.03.2012 bis 30.04.2017
Y1  - 2018
U6  - https://doi.org/10.2314/GBV:1024204243
ER  - 
TY  - JOUR
A1  - Adels, Klaudia
A1  - Monakhova, Yulia
T1  - Low-field NMR spectroscopic study of e-cigarettes: Is determination of only nicotine and organic carrier solvents possible?
JF  - Microchemical Journal
N2  - Electronic cigarettes (e-cigarettes) have become popular worldwide with the market growing exponentially in some countries. The absence of product standards and safety regulations requires urgent development of analytical methodologies for the holistic control of the growing diversity of such products. An approach based on low-field nuclear magnetic resonance (LF-NMR) at 80 MHz is presented for the simultaneous determination of key parameters: carrier solvents (vegetable glycerine (VG), propylene glycol (PG) and water), total nicotine as well as free-base nicotine fraction. Moreover, qualitative and quantitative determination of fourteen weak organic acids deliberately added to enhance sensory characteristics of e-cigarettes was possible. In most cases these parameters can be rapidly and conveniently determined without using any sample manipulation such as dilution, extraction or derivatization steps. The method was applied for 37 authentic e-cigarettes samples. In particular, eight different organic acids with the content up to 56 mg/mL were detected. Due to its simplicity, the method can be used in routine regulatory control as well as to study release behaviour of nicotine and other e-cigarettes constituents in different products.
KW  - Electronic cigarettes
KW  - Low field NMR
KW  - Carrier solvents
KW  - Free-base nicotine
KW  - Weak organic acids
Y1  - 2024
U6  - https://doi.org/10.1016/j.microc.2024.110859
SN  - 1095-9149
N1  - Corresponding author: Yulia Monakhova
VL  - 203
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - GEN
A1  - Tippkötter, Nils
A1  - Pasteur, A.
A1  - Ulber, Roland
T1  - Magnetisch abtrennbare Gold-Nanopartikel zur katalytischen Zuckeroxidation
T2  - Chemie Ingenieur Technik
N2  - Glucose ist ein primäres Zwischenprodukt der Verarbeitung nachwachsender Rohstoffe, wie z. B. Cellulose. Die wertsteigernde Weiterverarbeitung des Monosaccharids erfolgt häufig in Form vonFermentationsprozessen, jedoch kann der Rohstoff auch für zahlreiche chemische Verarbeitungsstufen genutzt werden. Ein großtechnisch relevanter Prozess ist die Herstellung von Gluconsäure (GS), die u. a. als Nahrungsmittelzusatz (E 574) eingesetzt wird. Die Darstellung der Säure erfolgt durch Oxidation von Glucose mit magnetisierbarem Gold-Nano-Katalysator. Die Rückgewinnung des Katalysators aus der Reaktionslösung wurde unter Einwirkung eines Magnetfeldgradienten verwirklicht. Die Synthese der magnetischen Goldkatalysatoren (sowohl Trägerpartikel als auch Gold-Nanopartikel) wurde durch nass-chemische Fällungsreaktionen durchgeführt. Die Charakteristiken der neuen Materialen konnte durch Messungen des PCD-Potenzials, Laserbeugung und REM/EDX untersucht werden. So wurden u. a. Partikeldurchmesser von 25 lm und ein Goldgehaltvon 1,03 % ermittelt. Weiterhin wurden für die Goldkatalysatoren optimale Reaktionsbedingungen für die Glucoseoxidation im geregelten Rührkesselreaktor etabliert. Hierdurch konnten eine Produktselektivität von 100 % und eine Wiederverwendbarkeit der Partikel über mindestens zehn Zyklen erreicht werden.
Y1  - 2012
U6  - https://doi.org/10.1002/cite.201250393
SN  - 0009-286X
SN  - 1522-2640 (eISSN)
N1  - ProcessNet-Jahrestagung 2012 und 30. DECHEMA-Jahrestagung der Biotechnologen, 10. – 13. September 2012, Karlsruhe
VL  - 84
IS  - 8
SP  - 1328
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Mang, Thomas
T1  - Magnetische Mikro- und Nanopartikel für Medizin und Biotechnik
JF  - Wässrige Kunststoffdispersionen und ihre Anwendung : Fachbereich Angewandte Naturwissenschaften und Technik / Fachhochschule Aachen. [Schriftleitung: Thomas Mang ...]
Y1  - 2005
N1  - FH-Texte ; 71 ; Makromolekulares Kolloquium FH Aachen
SP  - 137
EP  - 150
PB  - Fachhochschule Aachen
CY  - Aachen
ER  - 
TY  - GEN
A1  - Hering, T.
A1  - Pasteur, A.
A1  - Wollny, S.
A1  - Ulber, Roland
A1  - Tippkötter, Nils
T1  - Magnetische Separation von Gold-Nanopartikeln zur Gluconsäure-Produktion durch Hochgradient-Magnetseparation im Labormaßstab
T2  - Chemie Ingenieur Technik
N2  - Bei der Verarbeitung nachwachsender Rohstoffe entsteht aus Cellulose oder Stärke u. a. das wichtige Produkt Glucose. Diese niedermolekulare Kohlenhydratquelle wird üblicherweise als Substrat für biotechnologische und chemische Synthesen verwendet. Ein wirtschaftlich interessantes Oxidationsprodukt der Glucose ist Gluconsäure, die beispielsweise als Lebensmittelzusatzstoff (E 574), in der Medizin und Metallindustrie Verwendung findet. Die Umsetzung des Monosaccharids zu Gluconsäure erfolgt entweder durch mikrobielle Fermentation oder der Oxidation an heterogenen Katalysatoren. Die Zielsetzung der Studie ist die Untersuchung der Glucoseoxidation an magnetisierbaren Gold-Nanopartikeln unter nachfolgender Bypass-Separation des Katalysators mittels einer neuen Mini-HGMS-Einheit (Hochgradient-Magnetseparation). Dieser Filtertyp ermöglicht die selektive Trennung magnetischer Partikel aus Suspensionen mit hohem Feststoffgehalt oder Viskosität. Erste Ergebnisse zeigen eine Beladungskapazität des selbstkonstruierten Mini-HGMS von 550 mg goldbeschichteter magnetisierbarer Nanopartikel. Die Oxidation erfolgt bei einem pH-Wertvon 9, bei 40 °C und mit 100 mM Glucose in einem begasten Rührkesselreaktor. Das System soll zukünftig zum Katalysatorrecycling von hochviskosen und Feststoffbelasteten Produktströmen aus Bioraffinerien eingesetzt werden.
Y1  - 2014
U6  - https://doi.org/10.1002/cite.201450265
SN  - 0009-286X
SN  - 1522-2640 (eISSN)
N1  - ProcessNet-Jahrestagung 2014 und 31. DECHEMA-Jahrestagung der Biotechnologen, 30. September - 2. Oktober 2014, Eurogress Aachen
VL  - 86
IS  - 9
SP  - 1501
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Wollny, S.
A1  - Kampeis, P.
A1  - Oster, J.
A1  - Schneider, H.
A1  - Ulber, Roland
T1  - Magnetseparation von Proteinen : Separation von Zielmolekülen durch hochselektive Aptamere
JF  - GIT Labor-Fachzeitschrift
N2  - Durch die Kombination von Oligonukleotid-Liganden (Aptameren) hoher Bindungsaffinitäten
mit hochselektiv abtrennbaren magnetisierbaren Mikropartikeln
wird eine einstufige Separation von Zielmolekülen aus mikrobiologischen
Produktionsansätzen möglich. Die Aptamere werden hierfür reversibel
auf den Partikeloberflächen gebunden und für die spezifische Isolierung von
Bioprodukten eingesetzt. Die Abtrennung der beladenen Partikel erfolgt
durch einen neuen Rotor-Stator-Separator mit Hochgradient-Magnetfeld.
Y1  - 2011
VL  - 55
IS  - 10
SP  - 666
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Schmidt, Aaron C.
A1  - Turgut, Hatice
A1  - Le, Dao
A1  - Beloqui, Ana
A1  - Delaittre, Guillaume
T1  - Making the best of it: nitroxide-mediated polymerization of methacrylates via the copolymerization approach with functional styrenics
JF  - Polymer Chemistry
N2  - The SG1-mediated solution polymerization of methyl methacrylate (MMA) and oligo(ethylene glycol) methacrylate (OEGMA, Mₙ = 300 g mol⁻¹) in the presence of a small amount of functional/reactive styrenic comonomer is investigated. Moieties such as pentafluorophenyl ester, triphenylphosphine, azide, pentafluorophenyl, halide, and pyridine are considered. A comonomer fraction as low as 5 mol% typically results in a controlled/living behavior, at least up to 50% conversion. Chain extensions with styrene for both systems were successfully performed. Variation of physical properties such as refractive index (for MMA) and phase transition temperature (for OEGMA) were evaluated by comparing to 100% pure homopolymers. The introduction of an activated ester styrene derivative in the polymerization of OEGMA allows for the synthesis of reactive and hydrophilic polymer brushes with defined thickness. Finally, using the example of pentafluorostyrene as controlling comonomer, it is demonstrated that functional PMMA-b-PS are able to maintain a phase separation ability, as evidenced by the formation of nanostructured thin films.
Y1  - 2020
U6  - https://doi.org/10.1039/C9PY01458F
VL  - 11
IS  - 2
SP  - 593
EP  - 604
PB  - Royal Society of Chemistry (RSC)
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Jahnke, J.
T1  - Marine Planktonalgen der Arktis I. Die Haptophycee Phaeocystis pouchetii / Baumann, M. ; Jahnke, J.
JF  - Mikrokosmos. 75 (1986), H. 9
Y1  - 1986
SN  - 0026-3680
SP  - 262
EP  - 265
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Krämer, M.
T1  - Marine Planktonalgen der Arktis II. Diatomeen / Baumann, M. ; Krämer, M.
JF  - Mikrokosmos. 75 (1986), H. 10
Y1  - 1986
SN  - 0026-3680
SP  - 306
EP  - 310
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Künsch, M.
A1  - Stahlberg, R.
A1  - Steiger, F.
T1  - Massen- und Energiebilanz des Thermoselect-Verfahrens
JF  - Thermoselect-Verfahren zur Ent- und Vergasung von Abfällen / Franz J. Schweitzer [Hrsg.]
Y1  - 1994
SN  - 3-924511-47-0
PB  - EF-Verl. für Energie- u. Umwelttechnik
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Stahlberg, R.
A1  - Weisenburger, P.
A1  - Steiger, F.
T1  - Massen- und Energiebilanzen unter Einbeziehung der Untersuchungen an der Thermoselect-Anlage Fondotoce (Italien). Stahlberg, R.; Feuerriegel, U.; Weisenburger, P.; Steiger, F.
JF  - Thermoselect : der neue Weg, Restmüll umweltgerecht zu behandeln ; [Tagungsband zur Fachtagung Thermoselect am 19. Januar 1995 in Rastatt/Baden] / Günther Häßler. 2. durchges. und erw. Aufl.
Y1  - 1995
SN  - 3-9803665-4-5
N1  - Fachtagung THERMOSELECT ; (1995, Rastatt) ; Fachtagung Thermoselect ; (1995.01.19 : ; Rastatt)
SP  - 55
EP  - 84
PB  - Goerner
CY  - Karlsruhe
ER  - 
TY  - JOUR
A1  - Liu, Z.
A1  - Schaap, K. S.
A1  - Ballemans, L.
A1  - de Blois, E.
A1  - Rohde, M.
A1  - Paulßen, Elisabeth
T1  - Measurement of reaction kinetics of [177Lu]Lu-DOTA-TATE using a microfluidic system
JF  - Dalton Transactions
Y1  - 2017
U6  - https://doi.org/10.1039/C7DT01830D
SN  - 1477-9234
VL  - 46
IS  - 42
SP  - 14669
EP  - 14676
ER  -