TY  - JOUR
A1  - Selmer, Thorsten
A1  - Hermann, Gloria
A1  - Jessen, Holly
A1  - Gokarn, Ravi R.
T1  - Two beta-alanyl-CoA:ammonia lyases in Clostridium propionicum / Herrmann , G. ;  Selmer,  T. ; Jessen,  HJ. ; Gokarn,  RR. ; Selifonova,  O. ;  Gort , SJ. ; , Buckel, W.
JF  - The FEBS Journal. 272 (2005), H. 3
Y1  - 2005
SN  - 1742-464X
SP  - 813
EP  - 821
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Andrei, Paula I.
A1  - Pierik, Antonio J.
A1  - Zauner, Stefan
T1  - Subunit composition of the glycyl radical enzyme p-hydroxyphenylacetate decarboxylase. A small subunit, HpdC, is essential for catalytic activity / Andrei, PI. ;  Pierik,  AJ. ; Zauner , S. ; Andrei-Selmer, LC.  ; Selmer, T.
JF  - European Journal of Biochemistry. 271 (2004), H. 11
Y1  - 2004
SN  - 0014-2956
SP  - 2225
EP  - 2230
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Hetzel, Marc
A1  - Brock, Matthias
A1  - Pierik, Antonio J.
T1  - Acryloyl-CoA reductase from Clostridium propionicum. An enzyme complex of propionyl-CoA dehydrogenase and electron-transferring flavoprotein  / Hetzel, Marc ; Brock, Matthias ; Selmer, Thorsten, Pierik, Antonio J. ; Golding, Bernard T. ; Buckel, Wolfgang
JF  - European Journal of Biochemistry. 270 (2003), H. 5
Y1  - 2003
SN  - 0014-2956
SP  - 902
EP  - 910
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Willanzheimer, Angela
A1  - Hetzel, Marc
T1  - Propionate CoA-transferase from Clostridium propionicum. Cloning of the gene and identification of glutamate 324 at the active site
JF  - European Journal of Biochemistry. 269 (2002), H. 1
Y1  - 2002
SN  - 0014-2956
SP  - 372
EP  - 380
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Andrei, Paula I.
T1  - p-Hydroxyphenylacetate decarboxylase from Clostridium difficile. A novel glycyl radical enzyme catalysing the formation of p-cresol
JF  - European Journal of Biochemistry. 268 (2001), H. 5
Y1  - 2001
SN  - 0014-2956
SP  - 1363
EP  - 1372
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Darley, Dan J.
A1  - Clegg, William
A1  - Harrington, Ross W.
T1  - Stereocontrolled Synthesis of (2R,3S)-2-Methylisocitrate, a Central Intermediate in the Methylcitrate Cycle / Darley, Dan J. ; Selmer, Thorsten ; Clegg, William ; Harrington, Ross W. ; Buckel, Wolfgang ; Golding, Bernardt
JF  - Helvetica chimica acta. 86 (2003), H. 12
Y1  - 2003
SN  - 1522-2675
SP  - 3991
EP  - 3999
ER  - 
TY  - JOUR
A1  - Jeromin, Günter Erich
A1  - Zoor, Annegreth
T1  - A new irreversible enzyme-aided esterification method in organic solvents
JF  - Biotechnology letters. 30 (2008), H. 5
Y1  - 2008
SN  - 1573-6776
SP  - 925
EP  - 928
ER  - 
TY  - JOUR
A1  - Mang, Thomas
A1  - Hodenius, M. A. J.
A1  - Schmitz-Rode, T.
A1  - Baumann, M.
T1  - Absorption of 10-hydroxycamptothecin into the coat of magnetoliposomes / Hodenius, M. A. J. ; Schmitz-Rode, T. ; Baumann, M. ; Ivoanova, G. ; Wong, J. E. ; Mang, T. ; Haulena, F. ; Soenen, S. J. H. ; De Cuyper, M.
JF  - Colloids and Surfaces A: Physicochemical and Engineering Aspects. 343 (2009), H. 1-3
Y1  - 2009
SN  - 0927-7757
N1  - Colloid and Surface Science for Nanotechnology
SP  - 20
EP  - 23
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Mang, Thomas
A1  - Kolz, J.
A1  - Goga, N.
A1  - Casanova, F.
T1  - Spatial localization with single-sided NMR sensors / Kolz, J. ; Goga, N. ; Casanova, F. ; Mang, T. ; Blümich, B.
JF  - Applied Magnetic Resonance. 32 (2007), H. 1-2
Y1  - 2007
SN  - 0937-9347
SP  - 171
EP  - 184
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Jacobi, M.
A1  - Castillo, J.
A1  - Foerstel, D. H.
T1  - Ultra-low-level measurements of 3H and 14C in wines and champagne / Scherer, U. W. ; Jacobi, M. ; Castillo, J. ; Foerstel, D. H.
JF  - Radiation effects and defects in solids. 164 (2009), H. 5-6
Y1  - 2009
SN  - 1042-0150
SP  - 382
EP  - 385
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Santana, H. H. S.
A1  - Maier, G.
A1  - Rodenas, J.
T1  - Analysis of mechanical strength in ceramic pellets of nuclear fuel  / Santana, H. H. S. ; Maier, G. ; Scherer, U. W. ; Rodenas, J.
JF  - Radiation effects and defects in solids. 164 (2009), H. 5-6
Y1  - 2009
SN  - 1042-0150
SP  - 313
EP  - 318
PB  - Taylor & Francis
CY  - London
ER  - 
TY  - JOUR
A1  - Balakrishnan, Karthikeyan
A1  - Andrei-Selmer, Luminita-Cornelia
A1  - Selmer, Thorsten
A1  - Bacher, Michael
A1  - Dodel, Richard
T1  - Comparison of Intravenous Immunoglobulins for Naturally Occurring Autoantibodies against Amyloid-β
JF  - Journal of Alzheimer's Disease
Y1  - 2010
SN  - 1387-2877
VL  - 20
IS  - 1
SP  - 135
EP  - 143
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Pinkenburg, Olaf
T1  - Method of cloning at least one nucleic acid molecule of interest using type IIS restriction endonucleases, and corresponding cloning vectors, kits and system using type IIS restriction endonucleases / Selmer, Thorsten ; Pinkenburg, Olaf
Y1  - 2008
N1  - Patent No. WO 2008095927  	A1  	Aug 14, 2008
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
T1  - Identification of marker peptides in hair for discrimination with regard to species and breed / Koerner, Andrea ; Ring, Maike ; Fabry, Marlies ; Elbers, Gereon ; Moeller, Martin
JF  - Proceedings of the Aachen-Dresden International Textile Conference, 3rd, Aachen, Germany, Nov. 26-27, 2009
Y1  - 2009
N1  - Aachen-Dresden International Textile Conference ; (3 : ; 2009.11.26-27 : ; Aachen)
PB  - DWI
CY  - Aachen
ER  - 
TY  - JOUR
A1  - Mang, Thomas
A1  - Kolz, J.
A1  - Hunger, K.
A1  - Rath, S.
T1  - Investigation of self-diffusion processes in ionic hydrogels with a single-sided sensor / Kolz, J. ; Hunger, K. ; Rath, S. ; Mang, T. ;  Blümich, B.
JF  - Magnetic Resonance Imaging. 25 (2007), H. 4
Y1  - 2007
SN  - 0730-725X
N1  - Proceedings of the Eighth International Bologna Conference on Magnetic Resonance in Porous Media
SP  - 566
EP  - 567
ER  - 
TY  - JOUR
A1  - Bäcker, Matthias
A1  - Beging, Stefan
A1  - Biselli, Manfred
A1  - Poghossian, Arshak
A1  - Wang, J.
A1  - Zang, Werner
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Concept for a solid-state multi-parameter sensor system for cell-culture monitoring
JF  - Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI
Y1  - 2009
SN  - 0013-4686
SP  - 6107
EP  - 6112
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Jeromin, Günter Erich
T1  - Superabsorbed alcohol dehydrogenase—a new catalyst for asymmetric reductions
JF  - Biotechnology Letters. 31 (2009), H. 11
Y1  - 2009
SN  - 0141-5492
SP  - 1717
EP  - 1721
ER  - 
TY  - JOUR
A1  - Werner, Frederik
A1  - Krumbe, Christoph
A1  - Schumacher, Katharina
A1  - Groebel, Simone
A1  - Spelthahn, Heiko
A1  - Stellberg, Michael
A1  - Wagner, Torsten
A1  - Yoshinobu, Tatsuo
A1  - Selmer, Thorsten
A1  - Keusgen, Michael
A1  - Baumann, Marcus
A1  - Schöning, Michael Josef
T1  - Determination of the extracellular acidification of Escherichia coli by a light-addressable potentiometric sensor
JF  - Physica status solidi (a) : applications and material science. 208 (2011), H. 6
Y1  - 2011
SN  - 1862-6319
SP  - 1340
EP  - 1344
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Martins, Berta M.
A1  - Blaser, Martin
A1  - Feliks, Mikolaj
A1  - Ullmann, Matthias G.
A1  - Buckel, Wolfgang
A1  - Selmer, Thorsten
T1  - Structural basis for a Kolbe-type decarboxylation catalyzed by a glycyl radical enzyme
JF  - Journal of the American Chemical Society
Y1  - 2011
N1  - Just accepted manuscript
SP  - 1
EP  - 33
PB  - ACS Publications
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Schiffels, Johannes
A1  - Baumann, Marcus
A1  - Selmer, Thorsten
T1  - Facile analysis of short-chain fatty acids as 4-nitrophenyl esters in complex anaerobic fermentation samples by high performance liquid chromatography
JF  - Journal of Chromatography A. 1218 (2011), H. 34
Y1  - 2011
SN  - 0021-9673
SP  - 5848
EP  - 5851
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Srivastava, A.
A1  - Singh, V.
A1  - Aggarwal, P.
A1  - Schneeweiss, F.
A1  - Scherer, Ulrich W.
A1  - Friedrich, W.
T1  - Optical studies of insulating polymers for radiation dose monitoring
JF  - Indian Journal of Pure & Applied Physics
Y1  - 2010
SN  - 0019-5596
N1  - Special Issue: SI
VL  - 48
IS  - 11
SP  - 782
EP  - 786
ER  - 
TY  - JOUR
A1  - Bäcker, Matthias
A1  - Delle, L.
A1  - Poghossian, Arshak
A1  - Biselli, Manfred
A1  - Zang, Werner
A1  - Wagner, P.
A1  - Schöning, Michael Josef
T1  - Electrochemical sensor array for bioprocess monitoring
JF  - Electrochimica Acta (2011)
Y1  - 2011
VL  - 56
IS  - 26
SP  - 9673
EP  - 9678
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Aggarwal, Prerna
A1  - Singh, Virendra
A1  - Singh, Arjun
A1  - Scherer, Ulrich W.
A1  - Singh, Tejvir
A1  - Singla, Madan L.
A1  - Srivastava, Alok
T1  - Physico-chemical transformations in swift heavy ion modified poly(ethyleneterephthalate)
JF  - Radiation Physics and Chemistry
Y1  - 2011
SN  - 0969-806X
N1  - In Press, Accepted Manuscript
SP  - 1
EP  - 28
PB  - Pergamon Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Öhlschläger, Peter
A1  - Michel, Nico
A1  - Osen, Wolfram
A1  - Freyschmidt, Eva-Jasmin
T1  - T cell response to human papillomavirus 16 E7 in mice: comparison of Cr release assay, intracellular IFN-gamma production, ELISPOT and tetramer staining / Michel, Nico ; Öhlschläger, Peter ; Osen, Wolfram ; Freyschmidt, Eva-Jasmin ; Gutöhrlein, Heidrun ;
JF  - Intervirology. 45 (2002)
Y1  - 2002
SN  - 1423-0100
SP  - 290
EP  - 299
ER  - 
TY  - JOUR
A1  - Öhlschläger, Peter
A1  - Osen, Wolfram
A1  - Dell, Kerstin
A1  - Faath, Stefan
T1  - Human papillomavirus type 16 L1 capsomeres induce L1-specific cytotoxic T lymphocytes and tumor regression in C57BL/6 mice / Öhlschläger, Peter ; Osen, Wolfram ; Dell, Kerstin ; Faath, Stefan ; Garcea Robert L: ; Jochmus, Ingrid ; Müller, Martin, Pawlita,
JF  - Journal of Virology. 77 (2003), H. 8
Y1  - 2003
SN  - 1098-5514
SP  - 4635
EP  - 4645
ER  - 
TY  - JOUR
A1  - Öhlschläger, Peter
A1  - Corvinus, Florian M.
A1  - Orth, Carina
A1  - Moriggl, Richard
T1  - Persistent STAT3 activation in colon cancer is associated with enhanced cell proliferation and tumor growth / Corvinus, Florian, Moriggl, Richard ; Tsareva, Svetlana A. ; Wagner, Stefan ; Pfitzner, Edith B. ; Baus, Daniela ; Kaufmann, Roland : Huber, Luka
JF  - Neoplasia. 7 (2005), H. 6
Y1  - 2005
SN  - 1476-5586
SP  - 545
EP  - 555
ER  - 
TY  - JOUR
A1  - Öhlschläger, Peter
A1  - Quetting, Michael
A1  - Alvarez, Gerardo
A1  - Dürst, Matthias
A1  - Gissmann, Lutz
A1  - Kaufmann, Andreas M.
T1  - Enhancement of immunogenicity of a therapeutic cervical cancer DNA-based vaccine by co-application of sequence-optimized genetic adjuvants
JF  - International Journal of Cancer
Y1  - 2009
SN  - 1097-0215
VL  - 125
IS  - 1
SP  - 189
EP  - 198
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Oosterhuis, Koen
A1  - Öhlschläger, Peter
A1  - Berg, Joost H. van den
A1  - Toebes, Mireille
A1  - Gomez, Raquel
A1  - Schumacher, Ton N.
A1  - Haanen, John B.
T1  - Preclinical development of highly effective and safe DNA vaccines directed against HPV 16 E6 and E7
JF  - International Journal of Cancer
Y1  - 2011
SN  - 1097-0215
VL  - 129
IS  - 2
SP  - 397
EP  - 406
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Öhlschläger, Peter
A1  - Spies, Elmar
A1  - Alvarez, Gerardo
A1  - Quetting, Michael
A1  - Groettrup, Marcus
T1  - The combination of TLR-9 adjuvantation and electroporation-mediated delivery enhances in vivo antitumor responses after vaccination with HPV-16 E7 encoding DNA
JF  - International Journal of Cancer. 128 (2011), H. 2
Y1  - 2011
SN  - 1097-0215
SP  - 473
EP  - 481
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Leurs, Ulrike
A1  - Mezo, Gabor
A1  - Öhlschläger, Peter
A1  - Orban, Erika
A1  - Marquard, Andrea
A1  - Manea, Marilena
T1  - Design, synthesis, in vitro stability and cytostatic effect of multifunctional anticancer drug-bioconjugates containing GnRH-III as a targeting moiety
JF  - Peptide Science
N2  - Bioconjugates containing the GnRH-III hormone decapeptide as a targeting moiety are able to deliver chemotherapeutic agents specifically to cancer cells expressing GnRH receptors, thereby increasing their local efficacy while limiting the peripheral toxicity. However, the number of GnRH receptors on cancer cells is limited and they desensitize under continuous hormone treatment. A possible approach to increase the receptor mediated tumor targeting and consequently the cytostatic effect of the bioconjugates would be the attachment of more than one chemotherapeutic agent to one GnRH-III molecule. Here we report on the design, synthesis and biochemical characterization of multifunctional bioconjugates containing GnRH-III as a targeting moiety and daunorubicin as a chemotherapeutic agent. Two different drug design approaches were pursued. The first one was based on the bifunctional [4Lys]-GnRH-III (Glp-His-Trp-Lys-His-Asp-Trp-Lys-Pro-Gly-NH2) containing two lysine residues in positions 4 and 8, whose ϵ-amino groups were used for the coupling of daunorubicin. In the second drug design, the native GnRH-III (Glp-His-Trp-Ser-His-Asp-Trp-Lys-Pro-Gly-NH2) was used as a scaffold; an additional lysine residue was coupled to the ϵ-amino group of 8Lys in order to generate two free amino groups available for conjugation of daunorubicin. The in vitro stability/degradation of all synthesized compounds was investigated in human serum, as well as in the presence of rat liver lysosomal homogenate. Their cellular uptake was determined on human breast cancer cells and the cytostatic effect was evaluated on human breast, colon and prostate cancer cell lines. Compared with a monofunctional compound, both drug design approaches resulted in multifunctional bioconjugates with increased cytostatic effect.
Y1  - 2012
U6  - http://dx.doi.org/10.1002/bip.21640
SN  - 1097-0282
VL  - 98
IS  - 1
SP  - 1
EP  - 10
PB  - Wiley
CY  - New York, NY
ER  - 
TY  - JOUR
A1  - Manea, Marilena
A1  - Leurs, Ulrike
A1  - Orban, Erika
A1  - Baranyai, Zsuzsa
A1  - Öhlschläger, Peter
A1  - Marquardt, Andreas
A1  - Schulcz, Akos
A1  - Tejeda, Miguel
T1  - Enhanced Enzymatic Stability and Antitumor Activity of Daunorubicin-GnRH-III Bioconjugates Modified in Position 4
JF  - Bioconjugate Chemistry
Y1  - 2011
SN  - 1520-4812
VL  - 22
IS  - 7
SP  - 1320
EP  - 1329
PB  - ACS
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Spiess, Elmar
A1  - Wilfried, Reichardt
A1  - Alvarez, Gerardo
A1  - Gottrup, Marcus
A1  - Öhlschläger, Peter
T1  - An Artificial PAP Gene Breaks Self-tolerance and Promotes Tumor Regression in the TRAMP Model for Prostate Carcinoma
JF  - Molecular Therapy
Y1  - 2011
SN  - 1525-0016
VL  - 20
IS  - 3
SP  - 555
EP  - 564
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Öhlschläger, Peter
A1  - Osen, Wolfram
A1  - Peiler, Tanja
A1  - Caldeira, Sandra
T1  - A DNA vaccine based on a shuffled E7 oncogene of the human papillomavirus type 16 (HPV 16) induces E7-specific cytotoxic T cells but lacks transforming activity / Osen, Wolfram ; Peiler, Tanja ; Öhlschläger, Peter ; Caldeira, Sandra ; Faath, Stefan ; Mich
JF  - Vaccine. 19 (2001), H. 20
Y1  - 2001
SN  - 0264-410X
SP  - 4276
EP  - 4286
ER  - 
TY  - JOUR
A1  - Öhlschläger, Peter
A1  - Steinberg, Thorsten
A1  - Sehr, Peter
A1  - Osen, Wolfram
T1  - Modification of HPV 16 E7 genes: correlation between the level of protein expression and CTL response after immunization of C57BL/6 mice / Steinberg, Thorsten ; Öhlschläger, Peter ; Sehr, Peter ; Osen, Wolfram ; Gissmann, Lutz
JF  - Vaccine. 23 (2005), H. 9
Y1  - 2005
SN  - 0264-410X
SP  - 1149
EP  - 1157
ER  - 
TY  - JOUR
A1  - Öhlschläger, Peter
A1  - Pes, Michaela
A1  - Osen, Wolfram
A1  - Dürst, Matthias
T1  - An improved rearranged Human Papillomavirus Type 16 E7 DNA vaccine candidate (HPV-16 E7SH) induces an E7 wildtype-specific T cell response / Öhlschläger, Peter ; Pes, Michaela ; Osen, Wolfram ; Dürst, Matthias ; Schneider, Achim ; Gissmann, Lutz ; Kaufman
JF  - Vaccine. 24 (2006), H. 15
Y1  - 2006
SN  - 0264-410X
SP  - 2880
EP  - 2893
ER  - 
TY  - JOUR
A1  - Bäcker, Matthias
A1  - Raue, Markus
A1  - Schusser, Sebastian
A1  - Jeitner, C.
A1  - Breuer, L.
A1  - Wagner, P.
A1  - Poghossian, Arshak
A1  - Förster, Arnold
A1  - Mang, Thomas
A1  - Schöning, Michael Josef
T1  - Microfluidic chip with integrated microvalves based on temperature- and pH-responsive hydrogel thin films
JF  - Physica Status Solidi  (a)
N2  - Two types of microvalves based on temperature-responsive poly(N-isopropylacrylamide) (PNIPAAm) and pH-responsive poly(sodium acrylate) (PSA) hydrogel films have been developed and tested. The PNIPAAm and PSA hydrogel films were prepared by means of in situ photopolymerization directly inside the fluidic channel of a microfluidic chip fabricated by combining Si and SU-8 technologies. The swelling/shrinking properties and height changes of the PNIPAAm and PSA films inside the fluidic channel were studied at temperatures of deionized water from 14 to 36 °C and different pH values (pH 3–12) of Titrisol buffer, respectively. Additionally, in separate experiments, the lower critical solution temperature (LCST) of the PNIPAAm hydrogel was investigated by means of a differential scanning calorimetry (DSC) and a surface plasmon resonance (SPR) method. Mass-flow measurements have shown the feasibility of the prepared hydrogel films to work as an on-chip integrated temperature- or pH-responsive microvalve capable to switch the flow channel on/off.
Y1  - 2012
U6  - http://dx.doi.org/10.1002/pssa.201100763
SN  - 1862-6319
VL  - 209
IS  - 5
SP  - 839
EP  - 845
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Aggarwal, P.
A1  - Dhiman, S.
A1  - Kumar, G.
A1  - Scherer, Ulrich W.
A1  - Singla, M. L.
A1  - Srivastava, A.
T1  - Optical study of poly(ethyleneterephthalate) modified by different ionizing radiation dose
JF  - Indian Journal of Pure and Applied Physics
N2  - Thin films of poly(ethyleneterephthalate) [PET]were exposed to radiation dose ranging from 10 to 30 kGy by using gamma rays in the range 12.8-177.8 MGy using swift light ions of hydrogen. There was no effect of the radiation dose on the optical behaviour of PET as a result of exposure to radiation dose up to 30 kGy brought about by gamma rays but a significant decrease in the optical band gap values was observed when PET was exposed to swift light ions of hydrogen. The data obtained are discussed in terms of optical studies carried out on PET using swift heavy ions.
Y1  - 2012
SN  - 0019-5596
VL  - 50
IS  - 2
SP  - 129
EP  - 132
ER  - 
TY  - JOUR
A1  - Werner, Frederik
A1  - Groebel, Simone
A1  - Krumbe, Christoph
A1  - Wagner, Torsten
A1  - Selmer, Thorsten
A1  - Yoshinobu, Tatsuo
A1  - Baumann, Marcus
A1  - Schöning, Michael Josef
T1  - Nutrient concentration-sensitive microorganism-based biosensor
JF  - Physica Status Solidi (a)
Y1  - 2012
U6  - http://dx.doi.org/10.1002/pssa.201100801
SN  - 1862-6319
VL  - 209
IS  - 5
SP  - 900
EP  - 904
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Schiffels, Johannes
A1  - Pinkenburg, Olaf
A1  - Schelden, Maximilian
A1  - Aboulnaga, El-Hussiny A. A.
A1  - Baumann, Marcus
A1  - Selmer, Thorsten
T1  - An innovative cloning platform enables large-scale production and maturation of an oxygen-tolerant [NiFe]-hydrogenase from cupriavidus necator in Escherichia coli
JF  - PLOS one. 2013
Y1  - 2013
U6  - http://dx.doi.org/10.1371/journal.pone.0068812
SN  - 1932-6203
PB  - Public Library of Science
CY  - San Francisco, California
ER  - 
TY  - JOUR
A1  - Abulnaga, El-Hussiny
A1  - Pinkenburg, Olaf
A1  - Schiffels, Johannes
A1  - E-Refai, Ahmed
A1  - Buckel, Wolfgang
A1  - Selmer, Thorsten
T1  - Effect of an Oxygen-Tolerant Bifurcating Butyryl Coenzyme A Dehydrogenase/Electron-Transferring Flavoprotein Complex from Clostridium difficile on Butyrate Production in Escherichia coli
JF  - Journal of bacteriology
Y1  - 2013
SN  - 1098-5530 [E-Journal]
SN  - 0021-9193 [Print]
VL  - 195
IS  - 16
SP  - 3704
EP  - 3713
ER  - 
TY  - JOUR
A1  - Winckler, Silvia
A1  - Krueger, Rolf
A1  - Schnitzler, Thomas
A1  - Zang, Werner
A1  - Fischer, Rainer
A1  - Biselli, Manfred
T1  - A sensitive monitoring system for mammalian cell cultivation processes: a PAT approach
JF  - Bioprocess and biosystems engineering
N2  - Biopharmaceuticals such as antibodies are produced in cultivated mammalian cells, which must be monitored to comply with good manufacturing practice. We, therefore, developed a fully automated system comprising a specific exhaust gas analyzer, inline analytics and a corresponding algorithm to precisely determine the oxygen uptake rate, carbon dioxide evolution rate, carbon dioxide transfer rate, transfer quotient and respiratory quotient without interrupting the ongoing cultivation, in order to assess its reproducibility. The system was verified using chemical simulation experiments and was able to measure the respiratory activity of hybridoma cells and DG44 cells (derived from Chinese hamster ovary cells) with satisfactory results at a minimum viable cell density of ~2.0 × 10⁵ cells ml⁻¹. The system was suitable for both batch and fed-batch cultivations in bubble-aerated and membrane-aerated reactors, with and without the control of pH and dissolved oxygen.
Y1  - 2014
U6  - http://dx.doi.org/10.1007/s00449-013-1062-8
SN  - 1615-7591 (Print) 1615-7605 (Online)
VL  - 37
IS  - 5
SP  - 901
EP  - 912
PB  - Springer
CY  - Berlin, Heidelberg
ER  - 
TY  - JOUR
A1  - Meyer-Stork, L. Sebastian
A1  - Höcker, Hartwig
A1  - Berndt, Heinz
T1  - Syntheses and reactions of urethanes of cellobiose and cellulose-containing uretdione groups
JF  - Journal of applied polymer science
Y1  - 1992
SN  - 1097-4628
VL  - 44
IS  - 6
SP  - 1043
EP  - 1049
ER  - 
TY  - JOUR
A1  - Schnitzler, Thomas
T1  - Cultivation of hybridoma cell line CF-10H5 (DSMZ ACC477)
JF  - Application notes / Sartorius stedim biotech
Y1  - 2009
SP  - 1
EP  - 4
ER  - 
TY  - JOUR
A1  - Hemmerling, H.-J.
A1  - Merschenz-Quack, Angelika
A1  - Wunderlich, H.
T1  - Crystal structures of indeno[1,2-d]imidazoles. XIth European Crystallographic Meeting, Vienna 1988
JF  - Zeitschrift für Kristallographie - Crystalline Materials
Y1  - 1988
SN  - 2196-7105 (E-Books); 2194-4946 (Print)
VL  - 185
IS  - H. 1-4
SP  - 256
ER  - 
TY  - JOUR
A1  - Müller, Ulrike
A1  - Bongaerts, Johannes
A1  - Bovenberg, Roel
A1  - Jossek, Ralf
A1  - Krämer, Marco
A1  - Linnemann, J.
A1  - Müschen, S.
A1  - Ritterbecks, S.
A1  - Sprenger, G.
A1  - Wubbolts, Marcel
T1  - Metabolic engineering to produce fine chemicals in Escherichia coli
JF  - Mededelingen van de Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent
Y1  - 2001
SN  - 0035-533x
VL  - 66 (3a)
SP  - 215
EP  - 217
ER  - 
TY  - JOUR
A1  - Jossek, Ralf
A1  - Bongaerts, Johannes
A1  - Sprenger, Georg A.
T1  - Characterization of a new feedback-resistant 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase AroF of Escherichia coli
JF  - FEMS microbiology letters
Y1  - 2001
SN  - 1574-6968
VL  - Vol. 202
IS  - Iss. 1
SP  - 145
EP  - 148
ER  - 
TY  - JOUR
A1  - Bongaerts, Johannes
A1  - Krämer, Marco
A1  - Müller, Ulrike
A1  - Raeven, Leon
A1  - Wubbolts, Marcel
T1  - Metabolic engineering for microbial production of aromatic amino acids and derived compounds
JF  - Metabolic engineering
Y1  - 2001
SN  - 1096-7184 (E-Journal); 1096-7176 (Print)
VL  - Vol. 3
IS  - Iss. 4
SP  - 289
EP  - 300
ER  - 
TY  - JOUR
A1  - Schmitz, M.
A1  - Hirsch, E.
A1  - Bongaerts, Johannes
A1  - Takors, Ralf
T1  - Pulse experiments as a prerequisite for the quantification of in vivo enzyme kinetics in aromatic amino acid pathway of Eschericia coli
JF  - Biotechnology progress
Y1  - 2002
SN  - 1520-6033 (E-Journal); 8756-7938 (Print)
VL  - Vol. 18
IS  - Iss. 5
SP  - 935
EP  - 941
ER  - 
TY  - JOUR
A1  - Bongaerts, Johannes
A1  - Bovenberg, Roel
A1  - Krämer, Marco
A1  - Müller, Ulrike
A1  - Raeven, Leon
A1  - Wubbolts, Marcel
T1  - Metabolic engineering to produce fine chemicals in Escherichia coli
JF  - Chemie - Ingenieur - Technik (CIT)
Y1  - 2002
SN  - 1522-2640 (E-Journal); 0009-286X (Print)
N1  - Printausg. in der Bibliothek vorhanden: 63 ZS 022
VL  - Vol. 74
IS  - Iss. 5
SP  - 694
ER  - 
TY  - JOUR
A1  - Gerigk, M.
A1  - Bujnicki, R.
A1  - Ganpo-Nkwenkwa, E.
A1  - Bongaerts, Johannes
A1  - Sprenger, G.
A1  - Takors, Ralf
T1  - Process control for enhanced L-phenylalanine production using different recombinant Escherichia coli strains
JF  - Biotechnology and bioengineering
Y1  - 2002
SN  - 1097-0290 (E-Journal); 0006-3592 (Print)
VL  - Vol. 80
IS  - Iss. 7
SP  - 746
EP  - 754
ER  - 
TY  - JOUR
A1  - Gerigk, M.
A1  - Maaß, D.
A1  - Kreutzer, A.
A1  - Sprenger, G.
A1  - Bongaerts, Johannes
A1  - Wubbolts, Marcel
A1  - Takors, Ralf
T1  - Enhanced pilot-scale fed-batch L-phenylalanine production with recombinant Escherichia coli by fully integrated reactive extraction
JF  - Bioprocess and biosystems engineering
Y1  - 2002
SN  - 1432-0797 (E-Journal); 1615-7605 (E-Journal); 0178-515X (Print); 1615-7591 (Print)
VL  - Vol. 25
IS  - Iss. 1
SP  - 43
EP  - 52
ER  - 
TY  - JOUR
A1  - Krämer, Marco
A1  - Bongaerts, Johannes
A1  - Bovenberg, Roel
A1  - Kremer, Susanne
A1  - Müller, Ulrike
A1  - Orf, Sonja
A1  - Wubbolts, Marcel
A1  - Raeven, Leon
T1  - Metabolic engineering for microbial production of shikimic acid
JF  - Metabolic engineering
Y1  - 2003
SN  - 1096-7184 (E-Journal); 1096-7176 (Print)
VL  - Vol. 5
IS  - Iss. 4
SP  - 277
EP  - 283
ER  - 
TY  - JOUR
A1  - Polen, T.
A1  - Krämer, Marco
A1  - Bongaerts, Johannes
A1  - Wubbolts, Marcel
A1  - Wendisch, V. F.
T1  - The global gene expression response of Escherichia coli to L-phenylalanine
JF  - Journal of biotechnology
Y1  - 2005
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - Vol. 115
IS  - Iss. 3
SP  - 221
EP  - 237
ER  - 
TY  - JOUR
A1  - Unden, G.
A1  - Becker, S.
A1  - Bongaerts, Johannes
A1  - Schirawski, J.
A1  - Six, S.
T1  - Oxygen regulated gene expression in facultatively anaerobic bacteria
JF  - Antonie van Leeuwenhoek
Y1  - 1994
SN  - 0003-6072 (Print) ; 1572-9699 (online)
VL  - Vol. 66
IS  - Iss. 1-3
SP  - 3
EP  - 22
ER  - 
TY  - JOUR
A1  - Bongaerts, Johannes
A1  - Zoschke, Sascha
A1  - Weidner, Uwe
A1  - Linden, Gottfried
T1  - Transcriptional regulation of the proton translocating NADH
JF  - Molecular microbiology
Y1  - 1995
SN  - 1365-2958 (E-Journal); 0950-382x (Print)
VL  - Vol. 16
IS  - Iss. 3
SP  - 521
EP  - 534
ER  - 
TY  - JOUR
A1  - Unden, G.
A1  - Becker, S.
A1  - Bongaerts, Johannes
A1  - Holighaus, G.
A1  - Schirawski, J.
A1  - Six, S.
T1  - O2-sensing and O2-dependent gene regulation in facultatively anaerobic bacteria
JF  - Archives of microbiology
Y1  - 1995
SN  - 1432-072X (E-Journal); 0003-9276 (Print); 0302-8933 (Print)
VL  - Vol. 164
IS  - Iss. 2
SP  - 81
EP  - 90
ER  - 
TY  - JOUR
A1  - Wissenbach, U.
A1  - Six, S.
A1  - Bongaerts, Johannes
A1  - Ternes, D.
A1  - Steinwachs, S.
A1  - Unden, G.
T1  - A third periplasmic transport system for l-arginine in Escherichia coli: molecular characterization of the artPIQMJ genes, arginine binding and transport
JF  - Molecular microbiology
Y1  - 1995
SN  - 1365-2958 (E-Journal); 0950-382x (Print)
VL  - Vol. 17
IS  - Iss. 4
SP  - 675
EP  - 686
ER  - 
TY  - JOUR
A1  - Tran, Quang Hon
A1  - Bongaerts, Johannes
A1  - Vlad, Dorina
A1  - Unden, Gottfried
T1  - Requirement for the proton-pumping NADH dehydrogenase I of Escherichia coli in respiration of NADH to fumarate and its bioenergetic implications
JF  - European journal of biochemistry
Y1  - 1997
SN  - 0014-2956
VL  - Vol. 244
IS  - Iss. 1
SP  - 155
EP  - 160
ER  - 
TY  - JOUR
A1  - Unden, Gottfried
A1  - Bongaerts, Johannes
T1  - Alternative respiratory pathways of Escherichia coli: energetics and transcriptional regulation in response to electron acceptors
JF  - Biochimica et biophysica acta (BBA) - Bioenergetics
Y1  - 1997
SN  - 1879-2650 (E-Journal); 0005-2728 (Print)
VL  - Vol. 1320
IS  - Iss. 3
SP  - 217
EP  - 234
ER  - 
TY  - JOUR
A1  - Zientz, Evelyn
A1  - Bongaerts, Johannes
A1  - Unden, Gottfried
T1  - Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system
JF  - Journal of bacteriology
Y1  - 1998
SN  - 1098-5530 (E-Journal); 0021-9193 (Print)
VL  - Vol. 180
IS  - No. 20
SP  - 5421
EP  - 5425
ER  - 
TY  - JOUR
A1  - Wackwitz, B.
A1  - Bongaerts, Johannes
A1  - Goodman, S. D.
A1  - Unden, Gottfried
T1  - Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significance
JF  - Molecular and general genetics : MGG
Y1  - 1999
SN  - 1617-4623 (E-Journal); 1617-4615 (Print)
VL  - Vol. 262
IS  - Iss. 4 - 5
SP  - 876
EP  - 883
ER  - 
TY  - JOUR
A1  - Deppe, Veronika Maria
A1  - Bongaerts, Johannes
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
A1  - Meinhardt, Friedhelm
T1  - Enzymatic deglycation of Amadori products in bacteria
JF  - Applied microbiology and biotechnology
Y1  - 2011
SN  - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)
VL  - Vol. 90
IS  - Iss. 2
SP  - 399
EP  - 406
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Deppe, Veronika Maria
A1  - Klatte, Stephanie
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - O'Connell, Timothy
A1  - Meinhardt, Friedhelm
T1  - Genetic control of Amadori product degradation in Bacillus subtilis via regulation of frlBONMD expression by FrlR
JF  - Applied and environmental microbiology
Y1  - 2011
SN  - 1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)
VL  - Vol. 77
IS  - No. 9
SP  - 2839
EP  - 2846
PB  - American Society of Mechanical Engineers (ASME)
CY  - New York
ER  - 
TY  - JOUR
A1  - Bongaerts, Johannes
A1  - Esser, Simon
A1  - Lorbach, Volker
A1  - Al-Momani, Lóay
A1  - Müller, Michael A.
A1  - Franke, Dirk
A1  - Grondal, Christoph
A1  - Kurutsch, Anja
A1  - Bujnicki, Robert
A1  - Takors, Ralf
A1  - Raeven, Leon
A1  - Wubbolts, Marcel
A1  - Bovenberg, Roel
A1  - Nieger, Martin
A1  - Schürmann, Melanie
A1  - Trachtmann, Natalie
A1  - Kozak, Stefan
A1  - Sprenger, Georg A.
A1  - Müller, Michael
T1  - Diversity-oriented production of metabolites derived from chorismate and their use in organic synthesis
JF  - Angewandte Chemie International Edition
Y1  - 2011
SN  - 1521-3773 (E-Journal); 0570-0833 (Print); 1433-7851 (Print)
VL  - Vol. 50
IS  - Iss. 34
SP  - 7781
EP  - 7786
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Borgmeier, Claudia
A1  - Bongaerts, Johannes
A1  - Meinhardt, Friedhelm
T1  - Genetic analysis of the Bacillus licheniformis degSU operon and the impact of regulatory mutations on protease production
JF  - Journal of biotechnology
N2  - Disruption experiments targeted at the Bacillus licheniformis degSU operon and GFP-reporter analysis provided evidence for promoter activity immediately upstream of degU. pMutin mediated concomitant introduction of the degU32 allele – known to cause hypersecretion in Bacillus subtilis – resulted in a marked increase in protease activity. Application of 5-fluorouracil based counterselection through establishment of a phosphoribosyltransferase deficient Δupp strain eventually facilitated the marker-free introduction of degU32 leading to further protease enhancement achieving levels as for hypersecreting wild strains in which degU was overexpressed. Surprisingly, deletion of rapG – known to interfere with DegU DNA-binding in B. subtilis – did not enhance protease production neither in the wild type nor in the degU32 strain. The combination of degU32 and Δupp counterselection in the type strain is not only equally effective as in hypersecreting wild strains with respect to protease production but furthermore facilitates genetic strain improvement aiming at biological containment and effectiveness of biotechnological processes.
KW  - Marker-free mutagenesis
KW  - Extracellular enzymes
KW  - Uracil-phosphoribosyltransferase
KW  - Hypersecretion
Y1  - 2012
U6  - http://dx.doi.org/10.1016/j.jbiotec.2012.02.011
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - 159
IS  - 1-2
SP  - 12
EP  - 20
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Scheele, Sandra
A1  - Oertel, Dan
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Hellmuth, Hendrik
A1  - Maurer, Karl-Heinz
A1  - Bott, Michael
A1  - Freudl, Roland
T1  - Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol–xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum
JF  - Microbial biotechnology
Y1  - 2013
SN  - 1751-7915
SP  - 202
EP  - 206
PB  - Wiley-Blackwell
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Wilming, Anja
A1  - Begemann, Jens
A1  - Kuhne, Stefan
A1  - Regestein, Lars
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Maurer, Karl-Heinz
A1  - Büchs, Jochen
T1  - Metabolic studies of γ-polyglutamic acid production in Bacillus licheniformis by small-scale continuous cultivations
JF  - Biochemical engineering journal
Y1  - 2013
SN  - 1873-295X (E-Journal); 1369-703X (Print)
VL  - Vol. 73
SP  - 29
EP  - 37
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Voigt, Birgit
A1  - Schroeter, Rebecca
A1  - Jürgen, Britta
A1  - Albrecht, Dirk
A1  - Evers, Stefan
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - Schweder, Thomas
A1  - Hecker, Michael
T1  - The response of Bacillus licheniformis to heat and ethanol stress and the role of the SigB regulon
JF  - Proteomics
Y1  - 2013
SN  - 1615-9861 (E-Journal); 1615-9853 (Print)
VL  - Vol. 13
IS  - Iss. 14
SP  - 2140
EP  - 2146
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Rachinger, Michael
A1  - Bauch, Melanie
A1  - Strittmatter, Axel
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Maurer, Karl-Heinz
A1  - Daniel, Rolf
A1  - Liebl, Wolfgang
A1  - Liesegang, Heiko
A1  - Ehrenreich, Armin
T1  - Size unlimited markerless deletions by a transconjugative plasmid-system in Bacillus licheniformis
JF  - Journal of biotechnology
Y1  - 2013
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - Vol. 164
IS  - Iss. 4
SP  - 365
EP  - 369
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Wiegand, Sandra
A1  - Dietrich, Sascha
A1  - Hertel, Robert
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Volland, Sonja
A1  - Daniel, Rolf
A1  - Liesegang, Heiko
T1  - RNA-Seq of Bacillus licheniformis: active regulatory RNA features expressed within a productive fermentation
JF  - BMC genomics
Y1  - 2013
SN  - 1471-2164
VL  - Vol. 14
SP  - 667
PB  - BioMed Central
CY  - London
ER  - 
TY  - CHAP
A1  - Berndt, Heinz
A1  - Kalbe, Jochen
A1  - Kuropka, Rolf
A1  - Meyer-Stork, L. Sebastian
A1  - Höcker, Hartwig
ED  - Körner, Andrea
T1  - Progress and limitations of the DNA analysis in fine animal fiber identification
T2  - Proceedings of the 2nd International Symposium on Specialty Animal Fibers : Aachen, October 19 - 20, 1989. - (Schriftenreihe des Deutschen Wollforschungsinstituts an der Technischen Hochschule Aachen e. V. ; 106)
Y1  - 1990
SP  - 259
EP  - 265
PB  - Dt. Wollforschungsinst.
CY  - Aachen
ER  - 
TY  - JOUR
A1  - Kalbe, Jochen
A1  - Höcker, Hartwig
A1  - Berndt, Heinz
T1  - Design of enzyme reactors as chromatographic columns for racemic resolution of amino acid esters
JF  - Chromatographia
Y1  - 1989
SN  - 0009-5893
U6  - http://dx.doi.org/10.1007/BF02319646
VL  - 28
IS  - 3-4
SP  - 193
EP  - 196
ER  - 
TY  - JOUR
A1  - Kuropka, Rolf
A1  - Müller, Bettina
A1  - Höcker, Hartwig
A1  - Berndt, Heinz
T1  - Chiral stationary phases via hydrosilylation reaction of N-acryloylamino acids : I. Stationary phase with one chiral centre for high-performance liquid chromatography and development of a new derivatization pattern for amino acid enantiomers
JF  - Journal of chromatography A
Y1  - 1989
SN  - 0021-9673
IS  - 481
SP  - 380
EP  - 386
ER  - 
TY  - JOUR
A1  - Kalbe, Jochen
A1  - Kuropka, Rolf
A1  - Meyer-Stork, L. Sebastian
A1  - Berndt, Heinz
A1  - [u.a.], 
T1  - Isolation and characterization of high-molecular mass DNA from hair shafts
JF  - Biological chemistry
Y1  - 1988
SN  - 0177-3593
U6  - http://dx.doi.org/10.1515/bchm3.1988.369.1.413
VL  - 369
IS  - 1
SP  - 413
EP  - 416
ER  - 
TY  - JOUR
A1  - Heinze, Daniel
A1  - Mang, Thomas
A1  - Peter, Karin
A1  - Möller, Martin
A1  - Weichold, Oliver
T1  - Synthesis of low molecular weight poly(vinyl acetate) and its application as plasticizer
JF  - Journal of applied polymer science
N2  - Poly(vinyl acetate), PVAc, with a degree of polymerization Xn = 10 was prepared by chain-transfer radical polymerization using carbon tetrachloride and used as oligomeric plasticizer for commercial PVAc. However, the chlorinated chain ends cause a low thermal stability requiring mild Cl/H substitution. The product exhibits high thermal stability and excellent melt-compounding properties. Blends of oligomeric and commercial PVAc show single glass transition temperatures which decrease with higher oligomer content and exhibit small negative deviations from Fox' linear additivity rule. This indicates plasticization and miscibility being mainly due to entropic effects. Injection-moulded thick specimens show ductile behaviour at oligomer contents >10 wt %, while sheets with a thickness of 0.2–0.5 mm appear flexible already at 7.5 wt %. The oxygen permeability coefficients are an order of magnitude lower than those of low-density polyethylene. Due to the sum of their properties, the plasticized sheets present a promising alternative in the preparation of barrier materials.
Y1  - 2014
U6  - http://dx.doi.org/10.1002/app.40226
SN  - 1097-4628 (E-Journal); 0021-8995 (Print)
VL  - 131
IS  - 9
SP  - Article No. 40226
PB  - Wiley
CY  - New York
ER  - 
TY  - JOUR
A1  - Dutta, Suryendu
A1  - Hartkopf-Fröder, Christoph
A1  - Witte, Karin
A1  - Brocke, Rainer
A1  - Mann, Ulrich
T1  - Molecular characterization of fossil palynomorphs by transmission micro-FTIR spectroscopy: implications for hydrocarbon source evaluation
JF  - International journal of coal geology
Y1  - 2013
SN  - 1872-7840 (E-Journal); 0166-5162 (Print)
VL  - Vol. 115
SP  - 13
EP  - 23
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Takenaga, Shoko
A1  - Herrera, Cony F.
A1  - Werner, Frederik
A1  - Biselli, Manfred
A1  - Schnitzler, Thomas
A1  - Schöning, Michael Josef
A1  - Öhlschläger, Peter
A1  - Wagner, Torsten
T1  - Detection of the metabolic activity of cells by differential measurements based on a single light-addressable potentiometric sensor chip
T2  - 11. Dresdner Sensor-Symposium : 9.-11.12.2013
Y1  - 2013
SN  - 978-3-9813484-5-3
SP  - 63
EP  - 67
ER  - 
TY  - JOUR
A1  - Krüger, Götz
A1  - Grötzinger, Joachim
A1  - Berndt, Heinz
T1  - Enantiomeric resolution of amino acid derivatives on chiral stationary phases by high-performance liquid chromatography
JF  - Journal of Chromatography A
Y1  - 1987
U6  - http://dx.doi.org/10.1016/S0021-9673(01)85005-6
SN  - 0021-9673
VL  - 1987
IS  - 397
SP  - 223
EP  - 232
ER  - 
TY  - JOUR
A1  - Nokihara, Kiyoshi
A1  - Berndt, Heinz
T1  - Synthesis of hapten–polypeptide conjugates as antigen models for the N-terminal region of the α-2-chain of rabbit skin collagen
JF  - Journal of the Royal Society of Chemistry: Perkin Transactions 1
N2  - Synthesis of derivatives of the peptide sequence L-pyroglutamyl-L-phenylalanyl-L-aspartyl-glycyl-L-lysyl-glycyl-glycyl-glycine as the antigenic determinant representing the N-terminal non-helical region of the α-2-chain of rabbit skin collagen, and conjugation to two different polypeptide carriers, are described.
Y1  - 1978
U6  - http://dx.doi.org/10.1039/P19780000260
SN  - 1364-5463
SN  - 0300-922X
SN  - 1470-4358
VL  - 1978
IS  - 3
SP  - 260
EP  - 263
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - CHAP
A1  - Berndt, Heinz
A1  - Turck, Christoph W.
ED  - Voelter, Wolfgang
T1  - Syntheses of defined peptide derivatives by aminolysis of 3-[Nα-benzyloxycarbonyl peptidyloxy] -2-hydroxy-N-methyl-benzamides at elevated temperatures II. Synthesis of the peptide derivatives Z-Ala-X-Gly-N(Et)2, X=Phe, Leu, Val, Ser (But), Glu (OBut)
T2  - Chemistry of peptides and proteins : proceedings of the Fourth USSR-FRG Symposium, Tübingen, Federal Republic of Germany, June 8 - 12, 1982 / ed. Wolfgang Voelter ... - Vol. 2
Y1  - 1984
SN  - 3-11009-580-7
SP  - 97
EP  - 103
PB  - de Gruyter
CY  - Berlin [u.a.]
ER  - 
TY  - CHAP
A1  - Schnabel, Eberhard
A1  - Berndt, Heinz
ED  - Nesvadba, H.
T1  - Zur selektive Abspaltbarkeit der t-Butyloxycarbonylgruppe
T2  - Peptides 1971 : proceedings of the Eleventh European Peptide Symposium, Vienna, Austria, April 1971
Y1  - 1973
SN  - 0-7204-4120-X
SP  - 69
EP  - 70
PB  - North-Holland Publ. [u.a.]
CY  - Amsterdam [u.a.]
ER  - 
TY  - CHAP
A1  - Kroggel, Matthias
A1  - Berndt, Heinz
ED  - Ragnarsson, Ulf
T1  - The 0-hydroxiphenyloxicarbonyl-group a new base labile amine protecting group
T2  - Peptides 1984 : Proceedings of the 18th European Peptide Symposium Djurönäset, Sweden, June 10 - 15, 1984
Y1  - 1984
SN  - 91-22-00715-6
SP  - 81
EP  - 83
PB  - Almquist & Wiksell
CY  - Stockholm
ER  - 
TY  - JOUR
A1  - Naithani, V. K
A1  - Klostermeyer, Henning
A1  - Lange, H. R.
A1  - [u.a.], 
A1  - Berndt, Heinz
A1  - [u.a.], 
T1  - Preparation of peptide derivatives for porcine proinsulin-synthesis
JF  - Biological Chemistry
Y1  - 1971
U6  - http://dx.doi.org/10.1515/bchm2.1971.352.1.1
SN  - 1437-4315
VL  - 352
IS  - 1
SP  - 2
EP  - 3
PB  - De Gruyter
ER  - 
TY  - JOUR
A1  - Matoni, Georg
A1  - Berndt, Heinz
T1  - Thermal synthesis of the optical pure pentapeptide derivative Z-(L)-Ala-(L)-Phe-Gly-(L)-Phe-Gly-OMe
JF  - Tetrahedron letters
Y1  - 1980
U6  - http://dx.doi.org/10.1016/S0040-4039(00)93618-9
SN  - 0040-4039
VL  - 21
IS  - 1
SP  - 37
EP  - 40
ER  - 
TY  - JOUR
A1  - Nokihara, Kiyoshi
A1  - Berndt, Heinz
T1  - Studies on sulfur-containing peptides : tert-butyloxycarbonylsulfenyl and benzyloxycarbonylsulfenyl derivatives as protecting groups for cysteine
JF  - The journal of organic chemistry
Y1  - 1978
U6  - http://dx.doi.org/10.1021/jo00419a046
SN  - 0022-3263
VL  - 43
IS  - 25
SP  - 4893
EP  - 4895
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - JOUR
A1  - Danho, Waleed
A1  - Naithani, Vinod K.
A1  - Sasaki, André N.
A1  - Föhles, Joseph
A1  - Berndt, Heinz
A1  - [u.a.], 
T1  - Human proinsulin, VII : synthesis of two protected peptides corresponding to the sequences 1—45 and 46—86 of the prohormone
JF  - Hoppe-Seyler's Zeitschrift für physiologische Chemie
Y1  - 1980
U6  - http://dx.doi.org/10.1515/bchm2.1980.361.1.857
SN  - 1437-4315
SN  - 0018-4888
VL  - 361
IS  - 1
SP  - 857
EP  - 863
ER  - 
TY  - CHAP
A1  - Kalbe, Jochen
A1  - Kuropka, Rolf
A1  - Meyer-Stork, L. Sebastian
A1  - Lauter, S. L.
A1  - Höcker, Hartwig
A1  - Berndt, Heinz
ED  - Körner, Andrea
T1  - Identification of fine animal hair via DNA analysis
T2  - Proceedings of the 1st International Symposium on Specialty Animal Fibers : Aachen, October 26 - 27, 1987 ; [scientific, technological and economical aspects] . - (Schriftenreihe des Deutschen Wollforschungsinstitutes an der Technischen Hochschule Aachen e.V. ; 103)
Y1  - 1988
SN  - 0930-3723
SP  - 221
EP  - 227
PB  - Dt. Wollforschungsinst.
CY  - Aachen
ER  - 
TY  - JOUR
A1  - Berndt, Heinz
A1  - Krüger, Götz
T1  - Resolution of enantiomeric amino acid derivatives by high-performance liquid chromatography on chiral stationary phases
JF  - Journal of chromatography A
Y1  - 1985
U6  - http://dx.doi.org/10.1016/S0021-9673(01)92461-6
SN  - 0021-9673
VL  - 1985
IS  - 348
SP  - 275
EP  - 279
ER  - 
TY  - PAT
A1  - Berndt, Heinz
A1  - Höcker, Hartwig
A1  - Kuropka, Rolf
A1  - Kinkel, Joachim
T1  - Silane coated inorganic materials for chromatography : United States Patent
Y1  - 1991
PB  - [The United States Patent and Trademark Office]
CY  - [Alexandria, VA u.a.]
ER  - 
TY  - JOUR
A1  - Wang, Ren-Qi
A1  - Druckenmüller, Katharina
A1  - Elbers, Gereon
A1  - Guenther, Klaus
A1  - Croué, Jean-Philippe
T1  - Analysis of aquatic-phase natural organic matter by optimized LDI-MS method
JF  - Journal of mass spectrometry
N2  - The composition and physiochemical properties of aquatic-phase natural organic matter (NOM) are most important problems for both environmental studies and water industry. Laser desorption/ionization (LDI) mass spectrometry facilitated successful examinations of NOM, as humic and fulvic acids in NOM are readily ionized by the nitrogen laser. In this study, hydrophobic NOMs (HPO NOMs) from river, reservoir and waste water were characterized by this technique. The effect of analytical variables like concentration, solvent composition and laser energy was investigated. The exact masses of small molecular NOM moieties in the range of 200–1200 m/z were determined in reflectron mode. In addition, spectra of post-source-decay experiments in this range showed that some compounds from different natural NOMs had the same fragmental ions. In the large mass range of 1200–15 000 Da, macromolecules and their aggregates were found in HPO NOMs from natural waters. Highly humic HPO exhibited mass peaks larger than 8000 Da. On the other hand, the waste water and reservoir water mainly had relatively smaller molecules of about 2000 Da. The LDI-MS measurements indicated that highly humic river waters were able to form large aggregates and membrane foulants, while the HPO NOMs from waste water and reservoir water were unlikely to form large aggregates. Copyright © 2014 John Wiley & Sons, Ltd.
Y1  - 2014
U6  - http://dx.doi.org/10.1002/jms.3321
SN  - 1096-9888
VL  - 49
IS  - 2
SP  - 154
EP  - 160
PB  - Wiley
CY  - Bognor Regis
ER  - 
TY  - JOUR
A1  - Heine, A.
A1  - Herrmann, G.
A1  - Selmer, Thorsten
A1  - Terwesten, F.
A1  - Buckel, W.
A1  - Reuter, K.
T1  - High resolution crystal structure of clostridium propionicum β-Alanyl-CoA:Ammonia Lyase, a new member of the "Hot Dog Fold" protein superfamily
JF  - Proteins
N2  - Clostridium propionicum is the only organism known to ferment β-alanine, a constituent of coenzyme A (CoA) and the phosphopantetheinyl prosthetic group of holo-acyl carrier protein. The first step in the fermentation is a CoA-transfer to β-alanine. Subsequently, the resulting β-alanyl-CoA is deaminated by the enzyme β-alanyl-CoA:ammonia lyase (Acl) to reversibly form ammonia and acrylyl-CoA. We have determined the crystal structure of Acl in its apo-form at a resolution of 0.97 Å as well as in complex with CoA at a resolution of 1.59 Å. The structures reveal that the enyzme belongs to a superfamily of proteins exhibiting a so called “hot dog fold” which is characterized by a five-stranded antiparallel β-sheet with a long α-helix packed against it. The functional unit of all “hot dog fold” proteins is a homodimer containing two equivalent substrate binding sites which are established by the dimer interface. In the case of Acl, three functional dimers combine to a homohexamer strongly resembling the homohexamer formed by YciA-like acyl-CoA thioesterases. Here, we propose an enzymatic mechanism based on the crystal structure of the Acl·CoA complex and molecular docking. Proteins 2014; 82:2041–2053. © 2014 Wiley Periodicals, Inc.
Y1  - 2014
U6  - http://dx.doi.org/10.1002/prot.24557
SN  - 1097-0134 (E-Journal); 0887-3585 (Print)
VL  - 82
IS  - 9
SP  - 2041
EP  - 2053
PB  - Wiley-Liss
CY  - New York
ER  - 
TY  - JOUR
A1  - Takenaga, Shoko
A1  - Biselli, Manfred
A1  - Schnitzler, Thomas
A1  - Öhlschläger, Peter
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
T1  - Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis–Menten-like kinetics for cell culturing
JF  - Physica status solidi A : Applications and materials science
N2  - The metabolic activity of Chinese hamster ovary (CHO) cells was observed using a light-addressable potentiometric sensor (LAPS). The dependency toward different glucose concentrations (17–200 mM) follows a Michaelis–Menten kinetics trajectory with Kₘ = 32.8 mM, and the obtained Kₘ value in this experiment was compared with that found in literature. In addition, the pH shift induced by glucose metabolism of tumor cells transfected with the HPV-16 genome (C3 cells) was successfully observed. These results indicate the possibility to determine the tumor cells metabolism with a LAPS-based measurement device.
Y1  - 2014
U6  - http://dx.doi.org/10.1002/pssa.201330464
SN  - 1521-396X (E); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print)
VL  - 211
IS  - 6
SP  - 1410
EP  - 1415
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Whitehead, Mark
A1  - Öhlschläger, Peter
A1  - Almajhdi, Fahad N.
A1  - Alloza, Leonor
A1  - Marzábal, Pablo
A1  - Meyers, Ann E.
A1  - Hitzeroth, Inga I.
A1  - Rybicki, Edward P.
T1  - Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice
JF  - BMC cancer
Y1  - 2014
U6  - http://dx.doi.org/10.1186/1471-2407-14-367
SN  - 1471-2407
IS  - 14:367
SP  - 1
EP  - 15
PB  - BioMed Central
CY  - London
ER  - 
TY  - JOUR
A1  - Küppers, Tobias
A1  - Steffen, Victoria
A1  - Hellmuth, Hendrik
A1  - O'Connell, Timothy
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - Wiechert, Wolfgang
T1  - Developing a new production host from a blueprint: Bacillus pumilus as an industrial enzyme producer
JF  - Microbial cell factories
Y1  - 2014
U6  - http://dx.doi.org/10.1186/1475-2859-13-46
SN  - 1475-2859 (E-Journal)
VL  - 13
SP  - Article No. 46
PB  - BioMed Central
CY  - London
ER  - 
TY  - JOUR
A1  - Guo, Yuanyuan
A1  - Miyamoto, Ko-ichiro
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
A1  - Yoshinobu, Tatsuo
T1  - Theoretical study and simulation of light-addressable potentiometric sensors
JF  - Physica status solidi (A) : applications and materials
N2  - The light-addressable potentiometric sensor (LAPS) is a semiconductor-based potentiometric sensor using a light probe with an ability of detecting the concentration of biochemical species in a spatially resolved manner. As an important biomedical sensor, research has been conducted to improve its performance, for instance, to realize high-speed measurement. In this work, the idea of facilitating the device-level simulation, instead of using an equivalent-circuit model, is presented for detailed analysis and optimization of the performance of the LAPS. Both carrier distribution and photocurrent response have been simulated to provide new insight into both amplitude-mode and phase-mode operations of the LAPS. Various device parameters can be examined to effectively design and optimize the LAPS structures and setups for enhanced performance.
Y1  - 2014
U6  - http://dx.doi.org/10.1002/pssa.201330354
SN  - 0031-8965
VL  - 211
IS  - 6
SP  - 1467
EP  - 1472
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Smith, Walker O.
A1  - Baumann, Marcus
A1  - Wilson, David L.
A1  - Aletsee, Ludwig
T1  - Phytoplankton biomass and productivity in the marginal ice zone of the Fram Strait during summer 1984
JF  - Journal of geophysical research
Y1  - 1987
SN  - 2156-2202 (E-Journal); 2169-9291 (E-Journal); 0148-0227 (Print); 2169-9275 (Print)
VL  - Vol. 92
IS  - Iss. C7
SP  - 6777
EP  - 6786
ER  - 
TY  - JOUR
A1  - Jahnke, J.
A1  - Baumann, Marcus
T1  - Differentiation between Phaeocystis pouchetii (Har.) Lagerheim and Phaeocystis globosa Scherffel
JF  - Hydrobiological bulletin
Y1  - 1987
SN  - 0165-1404 (Print); 1573-5125 (E-Journal)
VL  - Vol. 21
IS  - Iss. 2
SP  - 141
EP  - 147
ER  - 
TY  - CHAP
A1  - Baumann, Marcus
ED  - Hempel, G.
T1  - Primary production and phytoplankton growth in the marginal ice zone of the Fram Strait and the interpretation of the field data with autecological experiments (Abstract)
T2  - Oceanography and biology of Arctic seas : selected papers from the ICES Symposium on Marine Sciences of the Arctic and Subarctic Regions, held in Santander, 28 - 30 September 1987. (Rapports et procès-verbaux des réunions / Conseil International pour l'Exploration de la Mer ; 188)
Y1  - 1989
SP  - 128
PB  - International Council for the Exploration of the Sea
CY  - Copenhagen
ER  - 
TY  - JOUR
A1  - Aletsee, Ludwig
A1  - Baumann, Marcus
T1  - A laboratory incubator equipped with facilities to automatically simulate natural irradiance
JF  - Boletim do Instituto Oceanográfico / Universidade de São Paulo
Y1  - 1991
SN  - 1982-436X; 0080-6331
VL  - Vol. 39
IS  - No. 2
SP  - 155
EP  - 159
ER  - 
TY  - JOUR
A1  - Tüg, Helmut
A1  - Baumann, Marcus
T1  - Problems of UV-B radiation measurements in biological research : critical remarks on current techniques and suggestions for improvements
JF  - Geophysical research letters
Y1  - 1994
SN  - 1944-8007 (E-Journal); 0094-8276 (Print)
VL  - Vol. 21
IS  - Iss. 8
SP  - 689
EP  - 692
ER  -