TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Turek, M.
A1  - Heiden, W.
A1  - Riesen, A.
A1  - Chhabda, T. A.
A1  - Schubert, J.
A1  - Krüger, P.
A1  - Keusgen, M.
T1  - Artificial intelligence/fuzzy logic method for analysis of combined signals from heavy metal chemical sensors
JF  - Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI
Y1  - 2009
SN  - 0013-4686
SP  - 6082
EP  - 6088
PB  - Elsevier
CY  - New York
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Simonis, A.
A1  - Krings, T.
A1  - Lüth, H.
A1  - Wang, J.
T1  - Evaluation of a chip-based thin-film / thick-film sensor hybrid for (bio-)chemical analysis
JF  - Electroanalysis
Y1  - 2002
SN  - 1040-0397
VL  - 14
IS  - 13
SP  - 955
EP  - 958
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Schütz, S.
T1  - Der Käfer/Chip-Sensor als bioelektronischer “Schnüffler”
JF  - Bioanalytik für Einsteiger : [Diabetes, Drogen und DNA] / Reinhard Renneberg. Darja Süßbier (Ill.). [Red.: Andreas Held ...]
Y1  - 2009
SN  - 978-3-8274-1831-9
SP  - 204
EP  - 205
PB  - Spektrum, Akad. Verl.
CY  - Heidelberg
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Schubert, Joachim P.
A1  - Kloock, Joachim P.
A1  - Zander, W.
A1  - Mourzina, Y. G.
A1  - Legin, A.
A1  - Vlasov, Y. G.
A1  - Lüth, H.
T1  - Innovative thin film techniques for microfabricating electrochemical sensors
JF  - Biocybernetics and Biomedical Engineering. 21 (2001), H. 4
Y1  - 2001
SN  - 0208-5216
SP  - 107
EP  - 119
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Schubert, J
A1  - Kloock, Joachim P.
A1  - Zander, W.
A1  - Mourzina, Y. G.
A1  - Legin, A.
A1  - Vlasov, Y. G.
A1  - Lüth, H.
T1  - Innovative thin film techniques for microfabricating electrochemical sensors
JF  - Lecture Notes of the ICB Seminars
Y1  - 2002
SP  - 55
EP  - 66
PB  - MCB
CY  - Warsaw
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Schroth, Peter
A1  - Lüth, Hans
A1  - Hummel, Hans E.
A1  - Schütz, Stefan
T1  - Insect chemoreceptors coupled to silicon transistors as innovative biosensors
JF  - Proceedings of SPIE. 4205 (2001)
Y1  - 2001
SP  - 152
EP  - 162
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Schroth, P.
A1  - Schütz, S.
A1  - Hummel, Hans E.
A1  - Lüth, H.
T1  - Connecting an insect antenna to a microchip as a novel type of biosensor
JF  - Proceedings : applications, trends, visions / VDE World Microtechnologies Congress, September 25 - 27, 2000, Expo 2000, Hannover, Germany. Organized by VDE Association for Electrical, Electronic & Information Technologies ....Vol 2.
Y1  - 2000
SN  - 3-8007-2579-7
N1  - MICRO.tec <1, 2000, Hannover>; auch als CD-ROM
SP  - 411
EP  - 416
PB  - VDE-Verl.
CY  - [Berlin] [u.a.]
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Schroth, P.
A1  - Schütz, S.
T1  - The use of insect chemoreceptors for the assembly of biosensors based on semiconductor field-effect sensors
JF  - Electroanalysis. 12 (2000), H. 9
Y1  - 2000
SN  - 1040-0397
SP  - 645
EP  - 652
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Schroth, P.
A1  - Hummel, Hans E.
A1  - Weißbecker, B.
A1  - Lüth, H.
A1  - Schütz, S.
T1  - Microcsaled living bioelectronic systems – Coupling beetles to silicon transducers
JF  - Chemical and biological sensors and analytical methods : proceedings of the international symposium / Sensor, Physical Electrochemistry, and Organic and Biological Electrochemistry Divisions. Ed.: M. Butler
Y1  - 2001
SN  - 1-56677-351-2
N1  - International Symposium: Chemical and Biological Sensors and Analytical Methods ; (2 : 2001.)
SP  - 165
EP  - 173
PB  - Electrochemical Society
CY  - Pennington, NJ
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Schmidt, C.
A1  - Schubert, J.
A1  - Zander, W.
A1  - Mesters, S.
A1  - Kordos, P.
A1  - Lüth, H.
A1  - Legin, A.
A1  - Seleznev, B.
A1  - Vlasov, Y. G.
T1  - Thin film sensors on the basis of chalcogenide glass materials prepared by pulsed laser deposition technique
JF  - Sensors and Actuators B. 68 (2000), H. 1-3
Y1  - 2000
SN  - 0925-4005
SP  - 254
EP  - 259
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Poghossian, Arshak
A1  - Yoshinobu, Tatsuo
A1  - Lüth, Hans
T1  - Semiconductor-based field-effect structures for chemical sensing
Y1  - 2001
SP  - 188
EP  - 198
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Poghossian, Arshak
A1  - Schultze, Joachim W.
T1  - Measuring seven parameters by two ISFET modules in a microcell set-up
JF  - Int. Journal of Computational Engineering Science. 4 (2003), H. 2
Y1  - 2003
SN  - 1465-8763
SP  - 257
EP  - 260
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Poghossian, Arshak
T1  - Detection of charged macromolecules by means of field-effect devices (FEDs): possibilities and limitations
JF  - Electrochemical sensors, biosensors and their biomedical applications / ed. by Xueji Zhang ...
Y1  - 2008
SN  - 978-0-12-373738-0
SP  - 187
EP  - 212
PB  - Elsevier Acad. Press
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Poghossian, Arshak
T1  - Silicon-based field-effect devices for (bio-)chemical sensing
JF  - International Conference on Advanced Semiconductor Devices and Microsystems, 2008. ASDAM 2008
Y1  - 2008
SN  - 978-1-4244-2325-5
SP  - 31
EP  - 38
CY  - Smolenice, Slovakia
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Poghossian, Arshak
T1  - Silicon-based field-effect devices with nanostructured surfaces for bio-/chemical sensing
JF  - Semiconductor micro- and nanoelectronics : Proceedings of the Seventh International Conference , Tsakhcadzor, Armenia July 3-5 2009
Y1  - 2009
SP  - 51
EP  - 53
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Poghossian, Arshak
T1  - BioFEDs (field-effect devices) : State-of-the-art and new directions
JF  - Electroanalysis
Y1  - 2006
U6  - http://dx.doi.org/10.1002/elan.200603609
SN  - 1521-4109
VL  - 18
IS  - 19-20
SP  - 1893
EP  - 1900
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Näther, Niko
A1  - Auger, V.
A1  - Poghossian, Arshak
A1  - Koudelka-Hep, M.
T1  - Miniaturised flow-through cell with integrated capacitive EIS sensor fabricated at wafer level using Si and SU-8 technologies
JF  - Sensors and Actuators B. 108 (2005), H. 1-2
Y1  - 2005
SN  - 0925-4005
N1  - Proceedings of the Tenth International Meeting on Chemical Sensors — IMCS - 10 2004
SP  - 986
EP  - 992
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Näther, Niko
A1  - Auger, V.
A1  - Poghossian, Arshak
A1  - Koudelka-Hep, M.
T1  - Miniaturized flow-through cell with integrated capacitive EIS sensors fabricated at wafer level using Si and Su-8 technologies
JF  - Technical digest of the 10th International Meeting on Chemical Sensors, July 11 - 14, 2004, Tsukuba, Japan / Japan Association of Chemical Sensors
Y1  - 2004
N1  - Chemical sensors ; 20.2004 Suppl. B. IMCS ; (10, 2004, Tsukuba) ; International Meeting on Chemical Sensors ; (10 : ; 2004.07.11-14 : ; Tsukuba)
SP  - 554
EP  - 555
PB  - Japan Association of Chemical Sensors
CY  - Fukuoka
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Mourzina, Yu.G.
A1  - Schubert, J.
A1  - Zander, W.
A1  - Legin, A.
A1  - Vlasov, Y. G.
A1  - Lüth, H.
T1  - Pulsed laser deposition – an innovative technique for preparing inorganic thin films
JF  - Electroanalysis. 13 (2001), H. 8-9
Y1  - 2001
SN  - 1040-0397
SP  - 727
EP  - 732
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Mourzina, Y. G.
A1  - Schubert, J.
A1  - Zander, W.
A1  - Legin, A.
A1  - Vlasov, Y. G.
A1  - Lüth, H.
T1  - Can pulsed laser deposition serve as an advanced technique in fabricating chemical sensors?
JF  - Sensors and Actuators B. 78 (2001), H. 1-3
Y1  - 2001
SN  - 0925-4005
N1  - Selected Papers form Eurosensors XIV
SP  - 273
EP  - 278
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Mourzina, Y. G.
A1  - Schubert, J.
A1  - Zander, W.
A1  - Legin, A.
A1  - Vlasov, Y. G.
A1  - Lüth, H.
T1  - Can pulsed laser deposition serve as an advanced technique in fabricating chemical sensors?
JF  - Proceedings : Copenhagen, Denmark, 27 - 30 August 2000 / [ed.: R. de Reus ...]
Y1  - 2000
SN  - 87-89935-50-0
N1  - Eurosensors ; (14, 2000, København) ; Eurosensors ; (14 : ; 2000.08.27-30 : ; Copenhagen) ; European Conference on Solid-State Transducers ; (14 : ; 2000.08.27-30 : ; Copenhagen)
SP  - 615
EP  - 618
PB  - MIC, Mikroelektronik Centret
CY  - Lyngby, Denmark
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Malkoc, Ü.
A1  - Thust, M.
A1  - Steffen, A.
A1  - Kordos, P.
A1  - Lüth, H.
T1  - Novel electrochemical sensors with structured and porous semiconductor/insulator capacitors
JF  - Sensors and Actuators B. 65 (2000), H. 1-3
Y1  - 2000
SN  - 0925-4005
SP  - 288
EP  - 290
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Lüth, H.
T1  - Novel concepts for silicon-based biosensors
JF  - Physica Status Solidi (A) (2001)
Y1  - 2001
SN  - 0031-8965
SP  - 65
EP  - 77
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Kurowski, A.
A1  - Thust, M.
A1  - Kordos, P.
A1  - Schultze, J. W.
A1  - Lüth, H.
T1  - Capacitive microsensors for biochemical sensing on porous silicon technology
JF  - Sensors and Actuators B. 64 (2000), H. 1-3
Y1  - 2000
SN  - 0925-4005
SP  - 59
EP  - 64
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Krause, R.
A1  - Block, K.
A1  - Musameh, M.
A1  - Mulchandani, A.
A1  - Wang, J.
T1  - A dual amperometric / potentiometric FIA-based biosensor for the distinctive detection of organophosphorus pesticides
JF  - Book of abstracts / ed. by J. Saneistr.
Y1  - 2002
SN  - 80-01-02576-4
N1  - Eurosensors ; (16, 2002, Praha)
SP  - 76
EP  - 79
PB  - Czech Technical University, Faculty of Electrical Engineering, Department of Measurement
CY  - Prague
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Krause, R.
A1  - Block, K.
A1  - Musahmeh, M.
A1  - Mulchandani, A.
A1  - Wang, J.
T1  - A dual amperometric/potentiometric FIA-based biosensor for the distinctive detection of organophosphorus pesticides
JF  - Sensors and Actuators B. 95 (2003), H. 1-3
Y1  - 2003
SN  - 0925-4005
SP  - 291
EP  - 296
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Kloock, Joachim P.
A1  - Knobbe, D.-T.
A1  - Räde, J.
A1  - Keusgen, M.
T1  - Silicon field-effect biosensor for cyanide detection
JF  - Technical digest of the 10th International Meeting on Chemical Sensors, July 11 - 14, 2004, Tsukuba, Japan / Japan Association of Chemical Sensors
Y1  - 2004
N1  - Chemical sensors ; 20.2004 Suppl. B. IMCS ; (10, 2004, Tsukuba) ; International Meeting on Chemical Sensors ; (10 : ; 2004.07.11-14 : ; Tsukuba)
SP  - 98
EP  - 99
PB  - Japan Association of Chemical Sensors
CY  - Fukuoka
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Kloock, Joachim P.
A1  - Knobbe, D.-T.
A1  - Krause, R.
A1  - Block, K.
A1  - Wang, J.
A1  - Mulchandani, A.
A1  - Keusgen, M.
T1  - Direktnachweis von Pestiziden und Cyanid mit elektrochemischen Enzymsensoren
JF  - Sensoren und Messsysteme 2004 : Tagung Ludwigsburg, 15. und 16. März 2004 / VDI-VDE-Gesellschaft Mess- und Automatisierungstechnik
Y1  - 2004
SN  - 3-18-091829-2
N1  - Auch erschienen als: VDI-Berichte ; 1829
SP  - 699
EP  - 706
PB  - VDI
CY  - Düsseldorf
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Kloock, Joachim P.
T1  - About 20 years of silicon-based thin-film sensors with chalcogenide glass materials for heavy metal analysis: Technological aspects of fabrication and miniaturization
JF  - Electroanalysis. 19 (2007), H. 19-20
Y1  - 2007
SN  - 1040-0397
SP  - 2029
EP  - 2038
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Kirchner, Patrick
A1  - Ng, Yue Ann
A1  - Spelthahn, Heiko
A1  - Schneider, Andreas
A1  - Henkel, Hartmut
A1  - Friedrich, Peter
A1  - Kolstad, Jens
A1  - Berger, Jörg
A1  - Keusgen, Michael
T1  - Gas sensor investigation based on a catalytically activated thin-film thermopile for H2O2 detection
JF  - Physica Status Solidi (A). 207 (2010), H. 4
Y1  - 2010
SN  - 1862-6300
N1  - Special Issue: Engineering of Functional Interfaces EnFI 2009
SP  - 787
EP  - 792
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Jünger, M.
A1  - Keusgen, M.
T1  - Knoblauchgehaltsbestimmungen mit einem feldeffektbasierten Halbleiterbiosensor
JF  - Sensoren und Messsysteme 2004 : Tagung Ludwigsburg, 15. und 16. März 2004 / VDI-VDE-Gesellschaft Mess- und Automatisierungstechnik
Y1  - 2004
SN  - 3-18-091829-2
N1  - Auch erschienen als: VDI-Berichte ; 1829
SP  - 707
EP  - 714
PB  - VDI
CY  - Düsseldorf
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Jacobs, M.
A1  - Muck, A.
A1  - Knobbe, D.-T.
A1  - Wang, J.
A1  - Chatrathi, M.
A1  - Spillmann, S.
T1  - Amperometric PDMS/glass capillary electrophoresis-based biosensor microchip for catechol and dopamin detection
JF  - Sensors and Actuators B. 108 (2005), H. 1-2
Y1  - 2005
SN  - 0925-4005
N1  - Proceedings of the Tenth International Meeting on Chemical Sensors — IMCS - 10 2004
SP  - 688
EP  - 694
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Hüllenkremer, B.
A1  - Glück, O.
A1  - Lüth, H.
A1  - Emons, H.
T1  - Voltohmmetry – a novel sensing principle for heavy metal determination in aqueous solutions
JF  - Sensors and Actuators B. 78 (2001), H. 1-3
Y1  - 2001
SN  - 0925-4005
SP  - 275
EP  - 280
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Bäcker, Matthias
T1  - Chip-basierte Sensoren für die Biotechnik
Y1  - 2012
SN  - 1611-0854
N1  - 4 Seiten
VL  - 13
IS  - 2
PB  - BIOCOM
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Buß, G.
A1  - Faßbender, F.
A1  - Glück, O.
A1  - Schmitt, G.
A1  - Schultze, J. W.
A1  - Lüth, H.
T1  - A silicon-based microelectrode array for chemical analysis
JF  - Sensors and Actuators B. 65 (2000), H. 1-3
Y1  - 2000
SN  - 0925-4005
SP  - 284
EP  - 287
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Bronder, Thomas
A1  - Wu, Chunsheng
A1  - Scheja, Sabrina
A1  - Jessing, Max
A1  - Metzger-Boddien, Christoph
A1  - Keusgen, Michael
A1  - Poghossian, Arshak
T1  - Label-Free DNA Detection with Capacitive Field-Effect Devices—Challenges and Opportunities
JF  - Proceedings
N2  - Field-effect EIS (electrolyte-insulator-semiconductor) sensors modified with a positively charged weak polyelectrolyte layer have been applied for the electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge. The EIS sensors are able to detect the existence of target DNA amplicons in PCR (polymerase chain reaction) samples and thus, can be used as tool for a quick verification of DNA amplification and the successful PCR process. Due to their miniaturized setup, compatibility with advanced micro- and nanotechnologies, and ability to detect biomolecules by their intrinsic molecular charge, those sensors can serve as possible platform for the development of label-free DNA chips. Possible application fields as well as challenges and limitations will be discussed.
Y1  - 2017
U6  - http://dx.doi.org/10.3390/proceedings1080719
SN  - 2504-3900
N1  - This article belongs to the Proceedings of "Proceedings of the 5th International Symposium on Sensor Science (I3S 2017)"
VL  - 1
IS  - 8
SP  - Artikel 719
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Brinkmann, D.
A1  - Rolka, David
A1  - Demuth, C.
A1  - Poghossian, Arshak
T1  - CIP (cleaning-in-place) suitable “non-glass” pH sensor based on a Ta2O5-gate EIS structure
JF  - Sensors and Actuators B: Chemical. 111-112 (2005)
Y1  - 2005
SN  - 0925-4005
N1  - Eurosensors XVIII 2004 - The 18th European Conference on Solid-State Transducers
SP  - 423
EP  - 429
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Brinkmann, D.
A1  - Demuth, C.
A1  - Poghossian, Arshak
T1  - CIP (cleaning-in-place)-suitable „non-glass“ pH sensor based on a Ta2O5-gate EIS structure
JF  - Digest of technical papers : September 12 - 15, 2004, Rome, Italy, Pontificia Universitas Sancto Thoma Aquinate in Urbe / [conference chairperson: C. Di Natale].
Y1  - 2004
SN  - 88-7621-282-5
N1  - Eurosensors ; (18, 2004, Roma)
SP  - 857
EP  - 860
CY  - Roma
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Biselli, Manfred
A1  - Selmer, Thorsten
A1  - Öhlschläger, Peter
A1  - Baumann, Marcus
A1  - Förster, Arnold
A1  - Poghossian, Arshak
T1  - Forschung „zwischen“ den Disziplinen: das Institut für Nano- und Biotechnologien
JF  - Analytik news : das Online-Labormagazin für Labor und Analytik
N2  - "Biologie trifft Mikroelektronik", das Motto des Instituts für Nano- und Biotechnologien (INB) an der FH Aachen, unterstreicht die zunehmende Bedeutung interdisziplinär geprägter Forschungsaktivitäten. Der thematische Zusammenschluss grundständiger Disziplinen, wie die Physik, Elektrotechnik, Chemie, Biologie sowie die Materialwissenschaften, lässt neue Forschungsgebiete entstehen, ein herausragendes Beispiel hierfür ist die Nanotechnologie: Hier werden neue Werkstoffe und Materialien entwickelt, einzelne Nanopartikel oder Moleküle und deren Wechselwirkung untersucht oder Schichtstrukturen im Nanometerbereich aufgebaut, die neue und vorher nicht bekannte Eigenschaften hervorbringen.

Vor diesem Hintergrund bündelt das im Jahre 2006 gegründete INB die an der FH Aachen vorhandenen Kompetenzen von derzeit insgesamt sieben Laboratorien auf den Gebieten der Halbleitertechnik und Nanoelektronik, Nanostrukturen und DNA-Sensorik, der Chemo- und Biosensorik, der Enzymtechnologie, der Mikrobiologie und Pflanzenbiotechnologie, der Zellkulturtechnik, sowie der Roten Biotechnologie synergetisch. In der Nano- und Biotechnologie steckt außergewöhnliches Potenzial! Nicht zuletzt deshalb stellen sich die Forscher der Herausforderung, in diesem Bereich gemeinsam zu forschen und Schnittstellen zu nutzen, um so bei der Gestaltung neuartiger Ideen und Produkte mitzuwirken, die zukünftig unser alltägliches Leben verändern werden.

Im Folgenden werden die verschiedenen Forschungsbereiche kurz zusammenfassend vorgestellt und vorhandene Interaktionen anhand von exemplarisch ausgewählten, aktuellen Forschungsprojekten skizziert.
Y1  - 2012
VL  - Publ. online
PB  - Dr. Beyer Internet-Beratung
CY  - Ober-Ramstadt
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Arzdorf, M.
A1  - Mulchandani, P.
A1  - Chen, W.
A1  - Mulchandani, A.
T1  - Towards a capacitive enzyme sensor for direct determination of organophosphorus pesticides: Fundamentals studies and aspects of development
JF  - Sensors. 3 (2003), H. 6
Y1  - 2003
SN  - 1424-8220
SP  - 119
EP  - 127
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Arzdorf, M.
A1  - Mulchandani, P.
A1  - Chen, W.
A1  - Mulchandani, A.
T1  - A capacitive field-effect sensor for the direct determination of organophosphorus pesticides
JF  - Sensors and Actuators B. 91 (2003), H. 1-3
Y1  - 2003
SN  - 0925-4005
SP  - 92
EP  - 97
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Abouzar, Maryam H.
A1  - Poghossian, Arshak
A1  - Han, Yinhua
A1  - Offenhäusser, Andreas
A1  - Ingebrandt, Sven
T1  - Markierungsfreie DNA-Detektion mit Silizium-Feldeffekt-Sensoren – Messeffekte oder Artefakte?
JF  - tm - Technisches Messen . 74 (2007), H. 9
Y1  - 2007
SN  - 0171-8096
SP  - 466
EP  - 476
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Abouzar, Maryam H.
A1  - Poghossian, Arshak
T1  - pH and ion sensitivity of a field-effect EIS (electrolyte-insulator-semiconductor) sensor covered with polyelectrolyte multilayers
JF  - Journal of Solid State Electrochemistry. 13 (2009), H. 1
Y1  - 2009
SN  - 1433-0768
N1  - This manuscript is an invited paper to the special issue on “Solid-state potentiometric sensors” (Guest editor: Johan Bobacka).
SP  - 115
EP  - 122
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Abouzar, Maryam H.
A1  - Ingebrandt, Sven
A1  - Platen, Johannes
A1  - Offenhäusser, Andreas
A1  - Poghossian, Arshak
ED  - Comini, Elisabetta
T1  - Towards label-free detection of charged macromolecules using field-effect-based structures : Scaling down from capacitive EIS sensor over ISFET to nano-scale devices
JF  - Nanostructured materials and hybrid composites for gas sensors and biomedical applications : symposium held April 18-20, 2006, San Francisco , California, U.S.A.
Y1  - 2006
SN  - 9781558998711
IS  - paper 0915-R05-04
SP  - 89
EP  - 94
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
T1  - Silicon-based biochemical sensors
JF  - CNI - The Center of Nanoelectronic Systems for Information Technology
Y1  - 2003
SP  - 165
EP  - 170
PB  - Foschungszentrum Jülich
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
T1  - "Playing around“ with field-effect sensors on the basis of EIS structures, LAPS and ISFETs
JF  - Sensors. 5 (2005), H. 3
Y1  - 2005
SN  - 1424-8220
SP  - 126
EP  - 138
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
T1  - "Voltohmmetry" – a new transducer principle for electrochemical sensors
JF  - Ultrathin electrochemical chemo- and biosensors : technology and performance / Vladimir M. Mirsky
Y1  - 2004
SN  - 3-540-21285-X
SP  - 117
EP  - 140
PB  - Springer
CY  - Berlin [u.a.]
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
T1  - Microelectronics contacts (bio-)chemistry – Semiconductor-based sensors
JF  - Biologi Italiani. 8 (2001)
Y1  - 2001
SN  - 0392-2510
SP  - 5
EP  - 10
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
T1  - Silicon-based field-effect structures – From dielectrics to bioelectronics
JF  - Dielectrics in emerging technologies : proceedings of the international symposium ; [papers presented at the First International Symposium on Science and Technology of Dielectrics in Emerging Fields held from 27th April to 2nd May 2003 in Paris, France] / sponsoring divisions: Dielectric Science and Technology, Electronics. Ed.: D. Misra.
Y1  - 2003
SN  - 1-56677-346-6
N1  - International Symposium on Science and Technology of Dielectrics in Emerging Fields ; (1, 2003, Paris)
SP  - 31
EP  - 37
PB  - Electrochemical Society
CY  - Pennington, NJ
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
T1  - Silicon recognizes biochemical parameters: Microchips for analytical sensor applications
JF  - American Laboratory. 32 (2000), H. 16
Y1  - 2000
SN  - 0044-7749
SP  - 24
EP  - 31
ER  - 
TY  - JOUR
A1  - Schusser, Sebastian
A1  - Poghossian, Arshak
A1  - Bäcker, Matthias
A1  - Leinhos, Marcel
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Characterization of biodegradable polymers with capacitive field-effect sensors
JF  - Sensors and actuators B: Chemical
N2  - In vitro studies of the degradation kinetic of biopolymers are essential for the design and optimization of implantable biomedical devices. In the presented work, a field-effect capacitive sensor has been applied for the real-time and in situ monitoring of degradation processes of biopolymers for the first time. The polymer-covered field-effect sensor is, in principle, capable to detect any changes in bulk, surface and interface properties of the polymer induced by degradation processes. The feasibility of this approach has been experimentally proven by using the commercially available biomedical polymer poly(D,L-lactic acid) (PDLLA) as a model system. PDLLA films of different thicknesses were deposited on the Ta₂O₅-gate surface of the field-effect structure from a polymer solution by means of spin-coating method. The polymer-modified field-effect sensors have been characterized by means of capacitance–voltage and impedance-spectroscopy method. The degradation of the PDLLA was accelerated by changing the degradation medium from neutral (pH 7.2) to alkaline (pH 9) condition, resulting in drastic changes in the capacitance and impedance spectra of the polymer-modified field-effect sensor.
KW  - Impedance spectroscopy
KW  - C–V method
KW  - Real-time monitoring
KW  - Poly(d,l-lacticacid)
KW  - (Bio)degradation
KW  - Field-effect sensor
Y1  - 2012
U6  - http://dx.doi.org/10.1016/j.snb.2012.07.099
SN  - 0925-4005
N1  - Part of special issue "Selected Papers from the 14th International Meeting on Chemical Sensors"
VL  - 187
SP  - 2
EP  - 7
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Schusser, Sebastian
A1  - Poghossian, Arshak
A1  - Bäcker, Matthias
A1  - Krischer, M.
A1  - Leinhos, Marcel
A1  - Wagner, P.
A1  - Schöning, Michael Josef
T1  - An application of field-effect sensors for in-situ monitoring of degradation of biopolymers
JF  - Sensors and actuators B: Chemical
N2  - The characterization of the degradation kinetics of biodegradable polymers is mandatory with regard to their proper application. In the present work, polymer-modified electrolyte–insulator–semiconductor (PMEIS) field-effect sensors have been applied for in-situ monitoring of the pH-dependent degradation kinetics of the commercially available biopolymer poly(d,l-lactic acid) (PDLLA) in buffer solutions from pH 3 to pH 13. PDLLA films of 500 nm thickness were deposited on the surface of an Al–p-Si–SiO2–Ta2O5 structure from a polymer solution by means of spin-coating method. The PMEIS sensor is, in principle, capable to detect any changes in bulk, surface and interface properties of the polymer induced by degradation processes. A faster degradation has been observed for PDLLA films exposed to alkaline solutions (pH 9, pH 11 and pH 13).
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.snb.2014.10.058
SN  - 1873-3077 (E-Journal); 0925-4005 (Print)
VL  - 207, Part B
SP  - 954
EP  - 959
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Schusser, Sebastian
A1  - Menzel, S.
A1  - Bäcker, Matthias
A1  - Leinhos, Marcel
A1  - Poghossian, Arshak
A1  - Wagner, P.
A1  - Schöning, Michael Josef
T1  - Degradation of thin poly(lactic acid) films: characterization by capacitance-voltage, atomic force microscopy, scanning electron microscopy and contact-angle measurements
JF  - Electrochimica Acta
Y1  - 2013
SN  - 1873-3859 (E-Journal); 0013-4686 (Print)
VL  - Vol. 113
SP  - 779
EP  - 784
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Schusser, Sebastian
A1  - Leinhos, Marcel
A1  - Bäcker, Matthias
A1  - Poghossian, Arshak
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Impedance spectroscopy: A tool for real-time in situ monitoring of the degradation of biopolymers
JF  - Physica Status Solidi (A)
N2  - Investigation of the degradation kinetics of biodegradable polymers is essential for the development of implantable biomedical devices with predicted biodegradability. In this work, an impedimetric sensor has been applied for real-time and in situ monitoring of degradation processes of biopolymers. The sensor consists of two platinum thin-film electrodes covered by a polymer film to be studied. The benchmark biomedical polymer poly(D,L-lactic acid) (PDLLA) was used as a model system. PDLLA films were deposited on the sensor structure from a polymer solution by using the spin-coating method. The degradation kinetics of PDLLA films have been studied in alkaline solutions of pH 9 and 12 by means of an impedance spectroscopy (IS) method. Any changes in a polymer capacitance/resistance induced by water uptake and/or polymer degradation will modulate the global impedance of the polymer-covered sensor that can be used as an indicator of the polymer degradation. The degradation rate can be evaluated from the time-dependent impedance spectra. As expected, a faster degradation has been observed for PDLLA films exposed to pH 12 solution.
Y1  - 2013
U6  - http://dx.doi.org/10.1002/pssa.201200941
SN  - 1521-396X ; 0031-8965
VL  - 210
IS  - 5
SP  - 905
EP  - 910
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Schusser, Sebastian
A1  - Krischer, Maximillian
A1  - Bäcker, Matthias
A1  - Poghossian, Arshak
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Monitoring of the Enzymatically Catalyzed Degradation of Biodegradable Polymers by Means of Capacitive Field-Effect Sensors
JF  - Analytical Chemistry
N2  - Designing novel or optimizing existing biodegradable polymers for biomedical applications requires numerous tests on the effect of substances on the degradation process. In the present work, polymer-modified electrolyte–insulator–semiconductor (PMEIS) sensors have been applied for monitoring an enzymatically catalyzed degradation of polymers for the first time. The thin films of biodegradable polymer poly(d,l-lactic acid) and enzyme lipase were used as a model system. During degradation, the sensors were read-out by means of impedance spectroscopy. In order to interpret the data obtained from impedance measurements, an electrical equivalent circuit model was developed. In addition, morphological investigations of the polymer surface have been performed by means of in situ atomic force microscopy. The sensor signal change, which reflects the progress of degradation, indicates an accelerated degradation in the presence of the enzyme compared to hydrolysis in neutral pH buffer media. The degradation rate increases with increasing enzyme concentration. The obtained results demonstrate the potential of PMEIS sensors as a very promising tool for in situ and real-time monitoring of degradation of polymers.
Y1  - 2015
U6  - http://dx.doi.org/10.1021/acs.analchem.5b00617
SN  - 1520-6882
VL  - 87
IS  - 13
SP  - 6607
EP  - 6613
PB  - ACS Publications
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Schusser, Sebastian
A1  - Krischer, M.
A1  - Molin, D. G. M.
A1  - Akker, N. M. S. van den
A1  - Bäcker, Matthias
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
T1  - Sensor System for in-situ and Real-time Monitoring of Polymer (bio) degradation
JF  - Procedia Engineering
N2  - A sensor system for investigating (bio)degradationprocesses of polymers is presented. The system utilizes semiconductor field-effect sensors and is capable of monitoring the degradation process in-situ and in real-time. The degradation of the polymer poly(d,l-lactic acid) is exemplarily monitored in solutions with different pH value, pH-buffer solution containing the model enzyme lipase from Rhizomucormiehei and cell-culture medium containing supernatants from stimulated and non-stimulated THP-1-derived macrophages mimicking activation of the immune system.
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.proeng.2015.08.815
SN  - 1877-7058
N1  - Eurosensors 2015
VL  - 120
SP  - 948
EP  - 951
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Schubert, J.
A1  - Schöning, Michael Josef
A1  - Mourzina, Y. G.
A1  - Legin, A. V.
A1  - Vlasov, Y. G.
A1  - Zander, W.
A1  - Lüth, H.
T1  - Multicomponent thin films for electrochemical sensor applications prepared by pulsed laser deposition
JF  - Sensors and Actuators B. 76 (2001), H. 1-3
Y1  - 2001
SN  - 0925-4005
SP  - 327
EP  - 330
ER  - 
TY  - JOUR
A1  - Schroth, P.
A1  - Weißbecker, B.
A1  - Schütz, S.
A1  - Ecken, H.
A1  - Yoshinobu, T.
A1  - Lüth, H.
A1  - Schöning, Michael Josef
T1  - Bioelectronic signal processing - intact chemoreceptors coupled to field-effect devices
JF  - Lecture Notes of the ICB Seminars
Y1  - 2002
SP  - 28
EP  - 42
PB  - MCB
CY  - Warsaw
ER  - 
TY  - JOUR
A1  - Schroth, P.
A1  - Weißbecker, B.
A1  - Schütz, S.
A1  - Ecken, H.
A1  - Yoshinobu, T.
A1  - Lüth, H.
A1  - Schöning, Michael Josef
T1  - Bioelectronic signal processing - intact chemoreceptors coupled to field-effect devices
JF  - Biocybernetics and Biomedical Engineering. 21 (2001), H. 3
Y1  - 2001
SN  - 0208-5216
SP  - 27
EP  - 42
ER  - 
TY  - JOUR
A1  - Schroth, P.
A1  - Schöning, Michael Josef
A1  - Schütz, S.
A1  - Steffen, A.
A1  - Weißbecker, B.
A1  - Hummel, Hans E.
A1  - Kordos, P.
A1  - Lüth, H.
T1  - Nachweis von organischen Duftstoffen durch Biosensoren auf der Basis von Insektenantennen
JF  - Gesellschaft Mess- und Automatisierungstechnik: Sensoren und Meßsysteme 2000 : Tagung Ludwigsburg, 13. und 14. März 2000 / VDI/VDE-Gesellschaft Meß- und Automatisierungstechnik
Y1  - 2000
SN  - 3-18-091530-7
N1  - VDI-Berichte; 1530
SP  - 107
EP  - 114
PB  - VDI-Verl.
CY  - Düsseldorf:
ER  - 
TY  - JOUR
A1  - Schroth, P.
A1  - Schöning, Michael Josef
A1  - Lüth, H.
A1  - Weißbecker, B.
A1  - Hummel, Hans E.
A1  - Schütz, S.
T1  - Extending the capabilities of an antenna/chip biosensor by employing various insect species
JF  - Sensors and  Actuators B. 78 (2001), H. 1-3
Y1  - 2001
SN  - 0925-4005
SP  - 1
EP  - 5
ER  - 
TY  - JOUR
A1  - Schroth, P.
A1  - Schöning, Michael Josef
A1  - Lüth, H.
A1  - Weißbecker, B.
A1  - Hummel, Hans E.
A1  - Schütz, S.
T1  - Extending the capabilities of an antenna/chip biosensor by employing various insect species
JF  - Proceedings : Copenhagen, Denmark, 27 - 30 August 2000 / [ed.: R. de Reus ...]
Y1  - 2000
SN  - 87-89935-50-0
N1  - Eurosensors ; (14, 2000, København) ; Eurosensors ; (14 : ; 2000.08.27-30 : ; Copenhagen) ; European Conference on Solid-State Transducers ; (14 : ; 2000.08.27-30 : ; Copenhagen)
SP  - 27
EP  - 30
PB  - MIC, Mikroelektronik Centret
CY  - Lyngby, Denmark
ER  - 
TY  - JOUR
A1  - Schroth, P.
A1  - Lüth, H.
A1  - Hummel, Hans E.
A1  - Schütz, S.
A1  - Schöning, Michael Josef
T1  - Characterising an insect antenna as a receptor for a biosensor by means of impedance spectroscopy
JF  - Scaling down in electrochemistry : electrochemical micro- and nanosystem technology ; proceedings of the 3rd International Symposium on Electrochemical Microsystem Technologies, Garmisch-Patenkirchen, Germany, 11 - 15 September 2000 / ed. by J. W. Schultz
Y1  - 2001
SN  - 0-08-044014-2
SP  - 293
EP  - 297
PB  - Elsevier [u.a.]
CY  - Amsterdam [u.a.]
ER  - 
TY  - JOUR
A1  - Schroeter, Rebecca
A1  - Hoffmann, Tamara
A1  - Voigt, Birgit
A1  - Meyer, Hanna
A1  - Bleisteiner, Monika
A1  - Muntel, Jan
A1  - Jürgen, Britta
A1  - Albrecht, Dirk
A1  - Becher, Dörte
A1  - Lalk, Michael
A1  - Evers, Stefan
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - Putzer, Harald
A1  - Hecker, Michael
A1  - Schweder, Thomas
A1  - Bremer, Erhard
T1  - Stress responses of the industrial workhorse Bacillus licheniformis to osmotic challenges
JF  - PLoS ONE
N2  - The Gram-positive endospore-forming bacterium Bacillus licheniformis can be found widely in nature and it is exploited in industrial processes for the manufacturing of antibiotics, specialty chemicals, and enzymes. Both in its varied natural habitats and in industrial settings, B. licheniformis cells will be exposed to increases in the external osmolarity, conditions that trigger water efflux, impair turgor, cause the cessation of growth, and negatively affect the productivity of cell factories in biotechnological processes. We have taken here both systems-wide and targeted physiological approaches to unravel the core of the osmostress responses of B. licheniformis. Cells were suddenly subjected to an osmotic upshift of considerable magnitude (with 1 M NaCl), and their transcriptional profile was then recorded in a time-resolved fashion on a genome-wide scale. A bioinformatics cluster analysis was used to group the osmotically up-regulated genes into categories that are functionally associated with the synthesis and import of osmostress-relieving compounds (compatible solutes), the SigB-controlled general stress response, and genes whose functional annotation suggests that salt stress triggers secondary oxidative stress responses in B. licheniformis. The data set focusing on the transcriptional profile of B. licheniformis was enriched by proteomics aimed at identifying those proteins that were accumulated by the cells through increased biosynthesis in response to osmotic stress. Furthermore, these global approaches were augmented by a set of experiments that addressed the synthesis of the compatible solutes proline and glycine betaine and assessed the growth-enhancing effects of various osmoprotectants. Combined, our data provide a blueprint of the cellular adjustment processes of B. licheniformis to both sudden and sustained osmotic stress.
Y1  - 2014
U6  - http://dx.doi.org/10.1371/journal.pone.0080956
SN  - 1932-6203
VL  - 8
IS  - 11
PB  - PLOS
CY  - San Francisco
ER  - 
TY  - JOUR
A1  - Scholl, Fabio
A1  - Morais, Paulo
A1  - Gabriel, Rayla
A1  - Schöning, Michael Josef
A1  - Siqueira, Jose Roberto, Jr.
A1  - Caseli, Luciano
T1  - Carbon nanotubes arranged as smart interfaces in lipid Langmuir-Blodgett films enhancing the enzymatic properties of penicillinase for biosensing applications
JF  - Applied Materials & Interfaces
N2  - In this paper, carbon nanotubes (CNTs) were incorporated in penicillinase-phospholipid Langmuir and Langmuir–Blodgett (LB) films to enhance the enzyme catalytic properties. Adsorption of the penicillinase and CNTs at dimyristoylphosphatidic acid (DMPA) monolayers at the air–water interface was investigated by surface pressure–area isotherms, vibrational spectroscopy, and Brewster angle microscopy. The floating monolayers were transferred to solid supports through the LB technique, forming mixed DMPA-CNTs-PEN films, which were investigated by quartz crystal microbalance, vibrational spectroscopy, and atomic force microscopy. Enzyme activity was studied with UV–vis spectroscopy and the feasibility of the supramolecular device nanostructured as ultrathin films were essayed in a capacitive electrolyte–insulator–semiconductor (EIS) sensor device. The presence of CNTs in the enzyme–lipid LB film not only tuned the catalytic activity of penicillinase but also helped conserve its enzyme activity after weeks, showing increased values of activity. Viability as penicillin sensor was demonstrated with capacitance/voltage and constant capacitance measurements, exhibiting regular and distinctive output signals over all concentrations used in this work. These results may be related not only to the nanostructured system provided by the film, but also to the synergism between the compounds on the active layer, leading to a surface morphology that allowed a fast analyte diffusion because of an adequate molecular accommodation, which also preserved the penicillinase activity. This work therefore demonstrates the feasibility of employing LB films composed of lipids, CNTs, and enzymes as EIS devices for biosensing applications.
Y1  - 2017
U6  - http://dx.doi.org/10.1021/acsami.7b08095
SN  - 1944-8252
VL  - 9
IS  - 36
SP  - 31054
EP  - 31066
PB  - ACS
CY  - Washington
ER  - 
TY  - JOUR
A1  - Schmitt, G.
A1  - Werner, C.
A1  - Schöning, Michael Josef
T1  - Microelectrochemical efficiency evaluation of inhibitors for CO2 corrosion of carbon steel under high shear stress gradients
JF  - Corrosion 2002. Conference Proceedings. NACExpo, 57th Annual Conference and Exhibition, Denver, US, Apr 7-11, 2002
Y1  - 2002
N1  - Paper 02280
SP  - 1
EP  - 11
PB  - NACE International
CY  - Houston, Texas
ER  - 
TY  - JOUR
A1  - Schmitt, G.
A1  - Faßbender, F.
A1  - Lüth, H.
A1  - Schöning, Michael Josef
A1  - Schultze, J. W.
A1  - Buß, G.
T1  - Passivation and corrosion of microelectrode arrays
JF  - Materials and Corrosion. 51 (2000), H. 1
Y1  - 2000
SN  - 0947-5117
SP  - 20
EP  - 25
ER  - 
TY  - JOUR
A1  - Schmidt, R.
A1  - Tonnesmann, A.
A1  - Förster, Arnold
A1  - Grimm, M.
A1  - Kordos, P.
A1  - Lüth, H.
T1  - Metamorphic InAlAs/InGaAs HEMT's on GaAs substrates using an InP buffer layer
JF  - 8th IEEE International Symposium on High Performance Electron Devices for Microwave and Optoelectronic Applications : EDMO 2000 ; [13 - 14 November 2000, University of Glasgow] / organised by Department of Electronics and Electrical Engineering, University of Glasgow, Glasgow Scotland. MTT/ED/AP/LEO Societies Joint Chapter, United Kingdom and Republic of Ireland Section. With technical co-sponsorship from IEEE Electron Device Society
Y1  - 2000
SN  - 0-7803-6550-X
N1  - EDMO <8, 2000, Glasgow> ; University <Glasgow> / Department of Electronics and Electrical Engineering ; IEEE catalog number 00TH8534
SP  - 95
EP  - 98
PB  - IEEE Operations Center
CY  - Piscataway, NJ
ER  - 
TY  - JOUR
A1  - Schiffels, Johannes
A1  - Selmer, Thorsten
T1  - A flexible toolbox to study protein-assisted metalloenzyme assembly in vitro
JF  - Biotechnology and Bioengineering
Y1  - 2015
U6  - http://dx.doi.org/10.1002/bit.25658
SN  - 1097-0290
VL  - 112
IS  - 11
SP  - 2360
EP  - 2372
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Schiffels, Johannes
A1  - Selmer, Thorsten
T1  - Combinatorial assembly of ferredoxin‐linked modules in Escherichia coli yields a testing platform for Rnf‐complexes
JF  - Biotechnology and Bioengineering
Y1  - 2019
U6  - http://dx.doi.org/10.1002/bit.27079
IS  - accepted article
SP  - 1
EP  - 36
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Schiffels, Johannes
A1  - Pinkenburg, Olaf
A1  - Schelden, Maximilian
A1  - Aboulnaga, El-Hussiny A. A.
A1  - Baumann, Marcus
A1  - Selmer, Thorsten
T1  - An innovative cloning platform enables large-scale production and maturation of an oxygen-tolerant [NiFe]-hydrogenase from cupriavidus necator in Escherichia coli
JF  - PLOS one. 2013
Y1  - 2013
U6  - http://dx.doi.org/10.1371/journal.pone.0068812
SN  - 1932-6203
PB  - Public Library of Science
CY  - San Francisco, California
ER  - 
TY  - JOUR
A1  - Scheele, Sandra
A1  - Oertel, Dan
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Hellmuth, Hendrik
A1  - Maurer, Karl-Heinz
A1  - Bott, Michael
A1  - Freudl, Roland
T1  - Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol–xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum
JF  - Microbial biotechnology
Y1  - 2013
SN  - 1751-7915
SP  - 202
EP  - 206
PB  - Wiley-Blackwell
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Scheele, S.
A1  - Bongaerts, Johannes
A1  - Maurer, K.-H.
A1  - Freudl, R.
T1  - Sekretion einer Kofaktor-haltigen Oxidase durch Corynebacterium glutamicum
JF  - Chemie - Ingenieur - Technik (CIT)
Y1  - 2009
SN  - 1522-2640 (E-Journal); 0009-286X (Print)
VL  - Vol. 81
IS  - Iss. 8
SP  - 1309
ER  - 
TY  - JOUR
A1  - Sabitova, A.
A1  - Ebert, Ph.
A1  - Lenz, A.
A1  - Schaafhausen, S.
A1  - Ivanova, L.
A1  - Dähne, M.
A1  - Hoffmann, A.
A1  - Dunin-Borkowski, R. E.
A1  - Förster, Arnold
A1  - Grandidier, B.
A1  - Eisele, H.
T1  - Intrinsic bandgap of cleaved ZnO(112¯0) surfaces
JF  - Applied physics letters
Y1  - 2013
SN  - 1077-3118 (E-Journal); 0003-6951 (Print)
VL  - Vol. 102
SP  - 021608
ER  - 
TY  - JOUR
A1  - Röhlen, Desiree
A1  - Pilas, Johanna
A1  - Schöning, Michael Josef
A1  - Selmer, Thorsten
T1  - Development of an amperometric biosensor platform for the combined determination of l-Malic, Fumaric, and l-Aspartic acid
JF  - Applied Biochemistry and Biotechnology
N2  - Three amperometric biosensors have been developed for the detection of L-malic acid, fumaric acid, and L -aspartic acid, all based on the combination of a malate-specific dehydrogenase (MDH, EC 1.1.1.37) and diaphorase (DIA, EC 1.8.1.4). The stepwise expansion of the malate platform with the enzymes fumarate hydratase (FH, EC 4.2.1.2) and aspartate ammonia-lyase (ASPA, EC 4.3.1.1) resulted in multi-enzyme reaction cascades and, thus, augmentation of the substrate spectrum of the sensors. Electrochemical measurements were carried out in presence of the cofactor β-nicotinamide adenine dinucleotide (NAD+) and the redox mediator hexacyanoferrate (III) (HCFIII). The amperometric detection is mediated by oxidation of hexacyanoferrate (II) (HCFII) at an applied potential of + 0.3 V vs. Ag/AgCl. For each biosensor, optimum working conditions were defined by adjustment of cofactor concentrations, buffer pH, and immobilization procedure. Under these improved conditions, amperometric responses were linear up to 3.0 mM for L-malate and fumarate, respectively, with a corresponding sensitivity of 0.7 μA mM−1 (L-malate biosensor) and 0.4 μA mM−1 (fumarate biosensor). The L-aspartate detection system displayed a linear range of 1.0–10.0 mM with a sensitivity of 0.09 μA mM−1. The sensor characteristics suggest that the developed platform provides a promising method for the detection and differentiation of the three substrates.
Y1  - 2017
U6  - http://dx.doi.org/10.1007/s12010-017-2578-1
SN  - 1559-0291
VL  - 183
SP  - 566
EP  - 581
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Röhlen, Desiree
A1  - Pilas, Johanna
A1  - Dahmen, Markus
A1  - Keusgen, Michael
A1  - Selmer, Thorsten
A1  - Schöning, Michael Josef
T1  - Toward a Hybrid Biosensor System for Analysis of Organic and Volatile Fatty Acids in Fermentation Processes
JF  - Frontiers in Chemistry
N2  - Monitoring of organic acids (OA) and volatile fatty acids (VFA) is crucial for the control of anaerobic digestion. In case of unstable process conditions, an accumulation of these intermediates occurs. In the present work, two different enzyme-based biosensor arrays are combined and presented for facile electrochemical determination of several process-relevant analytes. Each biosensor utilizes a platinum sensor chip (14 × 14 mm²) with five individual working electrodes. The OA biosensor enables simultaneous measurement of ethanol, formate, d- and l-lactate, based on a bi-enzymatic detection principle. The second VFA biosensor provides an amperometric platform for quantification of acetate and propionate, mediated by oxidation of hydrogen peroxide. The cross-sensitivity of both biosensors toward potential interferents, typically present in fermentation samples, was investigated. The potential for practical application in complex media was successfully demonstrated in spiked sludge samples collected from three different biogas plants. Thereby, the results obtained by both of the biosensors were in good agreement to the applied reference measurements by photometry and gas chromatography, respectively. The proposed hybrid biosensor system was also used for long-term monitoring of a lab-scale biogas reactor (0.01 m³) for a period of 2 months. In combination with typically monitored parameters, such as gas quality, pH and FOS/TAC (volatile organic acids/total anorganic carbonate), the amperometric measurements of OA and VFA concentration could enhance the understanding of ongoing fermentation processes.
Y1  - 2018
U6  - http://dx.doi.org/10.3389/fchem.2018.00284
IS  - 6
PB  - Frontiers
CY  - Lausanne
ER  - 
TY  - JOUR
A1  - Rosenauer, A.
A1  - Oberst, W.
A1  - Litvinov, D.
A1  - Gerthsen, D.
A1  - Förster, Arnold
A1  - Schmidt, R.
T1  - Structural and Chemical Investigation of In-0.6Ga0.4As Stranski-Krastanow Layers Burried in GaAs by Transmission Electron Microscopy
JF  - Physical Review B. 61 (2000), H. 12
Y1  - 2000
SN  - 1095-3795
SP  - 8276
EP  - 8288
ER  - 
TY  - JOUR
A1  - Rolka, David
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
T1  - Integration of a capacitive EIS sensor into a FIA system for pH and penicillin determination
JF  - Sensors. 4 (2004)
Y1  - 2004
SN  - 1424-8220
SP  - 84
EP  - 94
ER  - 
TY  - JOUR
A1  - Rodrigues, Raul T.
A1  - Morais, Paulo V.
A1  - Nordi, Cristina S. F.
A1  - Schöning, Michael Josef
A1  - Siqueira Jr., José R.
A1  - Caseli, Luciano
T1  - Carbon Nanotubes and Algal Polysaccharides To Enhance the Enzymatic Properties of Urease in Lipid Langmuir-Blodgett Films
JF  - Langmuir
N2  - Algal polysaccharides (extracellular polysaccharides) and carbon nanotubes (CNTs) were adsorbed on dioctadecyldimethylammonium bromide Langmuir monolayers to serve as a matrix for the incorporation of urease. The physicochemical properties of the supramolecular system as a monolayer at the air–water interface were investigated by surface pressure–area isotherms, surface potential–area isotherms, interfacial shear rheology, vibrational spectroscopy, and Brewster angle microscopy. The floating monolayers were transferred to hydrophilic solid supports, quartz, mica, or capacitive electrolyte–insulator–semiconductor (EIS) devices, through the Langmuir–Blodgett (LB) technique, forming mixed films, which were investigated by quartz crystal microbalance, fluorescence spectroscopy, and field emission gun scanning electron microscopy. The enzyme activity was studied with UV–vis spectroscopy, and the feasibility of the thin film as a urea sensor was essayed in an EIS sensor device. The presence of CNT in the enzyme–lipid LB film not only tuned the catalytic activity of urease but also helped to conserve its enzyme activity. Viability as a urease sensor was demonstrated with capacitance–voltage and constant capacitance measurements, exhibiting regular and distinctive output signals over all concentrations used in this work. These results are related to the synergism between the compounds on the active layer, leading to a surface morphology that allowed fast analyte diffusion owing to an adequate molecular accommodation, which also preserved the urease activity. This work demonstrates the feasibility of employing LB films composed of lipids, CNT, algal polysaccharides, and enzymes as EIS devices for biosensing applications.
Y1  - 2018
U6  - http://dx.doi.org/10.1021/acs.langmuir.7b04317
SN  - 1520-5827
VL  - 34
IS  - 9
SP  - 3082
EP  - 3093
PB  - ACS Publications
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Riemer, A.
A1  - Schroth, P.
A1  - Schütz, S.
A1  - Hummel, Hans E.
A1  - Lüth, H.
A1  - Kohl, C.-D.
A1  - Schöning, Michael Josef
T1  - The future of fire detection: Biological sensors? – Die Zukunft der Brandgassensorik: Biologische Sensoren?
JF  - Gassensorik in der Brandmeldetechnik : [VdS-Fachtagung, am 15. und 16. November 2000 in Köln] = Gas sensors for fire detection / VdS Schadenverhütung
Y1  - 2000
N1  - Fachtagung Gassensorik in der Brandmeldetechnik ; (2000, Köln)
SP  - 1
EP  - 7
PB  - VdS Schadenverhütung
CY  - Köln
ER  - 
TY  - JOUR
A1  - Ribitsch, D.
A1  - Karl, W.
A1  - Birner-Gruenberger, R.
A1  - Gruber, K.
A1  - Eiteljoerg, I.
A1  - Remler, P.
A1  - Wieland, S.
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
A1  - Schwab, H.
T1  - C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli
JF  - Journal of biotechnology
N2  - Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477.

This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7–11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.
Y1  - 2010
U6  - http://dx.doi.org/10.1016/j.jbiotec.2010.09.947
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - 150
IS  - 3
SP  - 408
EP  - 416
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Ribitsch, D.
A1  - Heumann, S.
A1  - Karl, W.
A1  - Gerlach, J.
A1  - Leber, R.
A1  - Birner-Gruenberger, R.
A1  - Gruber, K.
A1  - Eiteljoerg, I.
A1  - Remler, P.
A1  - Siegert, Petra
A1  - Lange, J.
A1  - Maurer, Karl-Heinz
A1  - Berg, G.
A1  - Guebitz, G. M.
A1  - Schwab, H.
T1  - Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli
JF  - Journal of biotechnology
N2  - A large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. Several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. Encapsulation of cells in alginate beads induced protease production. Stenotrophomonas maltophilia emerged as best performer under washing conditions. For identification of wash-active proteases, four extracellular serine proteases called StmPr1, StmPr2, StmPr3 and StmPr4 were cloned. StmPr2 and StmPr4 were sufficiently overexpressed in E. coli. Expression of StmPr1 and StmPr3 resulted in unprocessed, insoluble protein. Truncation of most of the C-terminal domain which has been identified by enzyme modeling succeeded in expression of soluble, active StmPr1 but failed in case of StmPr3.

From laundry application tests StmPr2 turned out to be a highly wash-active protease at 45 °C. Specific activity of StmPr2 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 17 ± 2 U/mg. In addition we determined the kinetic parameters and cleavage preferences of protease StmPr2.
KW  - Alginate beads
KW  - Stenotrophomonas maltophilia
KW  - Detergent protease
Y1  - 2012
U6  - http://dx.doi.org/10.1016/j.jbiotec.2011.09.025
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - 157
IS  - 1
SP  - 140
EP  - 147
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Reisert, Steffen
A1  - Schneider, Benno
A1  - Geissler, Hanno
A1  - Gompel, Matthias van
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Multi-sensor chip for the investigation of different types of metal oxides for the detection of H2O2 in the ppm range
JF  - physica status solidi (a)
N2  - In this work, a multi-sensor chip for the investigation of the sensing properties of different types of metal oxides towards hydrogen peroxide in the ppm range is presented. The fabrication process and physical characterization of the multi-sensor chip are described. Pure SnO2 and WO3 as well as Pd- and Pt-doped SnO2 films are characterized in terms of their sensitivity to H2O2. The sensing films have been prepared by drop-coating of water-dispensed nano-powders. A physical characterization, including scanning electron microscopy and X-ray diffraction analysis of the deposited metal-oxide films, was done. From the measurements in hydrogen peroxide atmosphere, it could be shown, that all of the tested metal oxide films are suitable for the detection of H2O2 in the ppm range. The highest sensitivity and reproducibility was achieved using Pt-doped SnO2.

Calibration plot of a SnO2, WO3, Pt-, and Pd-doped SnO2 gas sensor for H2O2 concentrations in the ppm range.
Y1  - 2013
SN  - 1862-6319
VL  - 210
IS  - 5
SP  - 898
EP  - 904
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Reisert, Steffen
A1  - Henkel, Hartmut
A1  - Schneider, Andreas
A1  - Schäfer, Daniel
A1  - Friedrich, Peter
A1  - Berger, Jörg
A1  - Schöning, Michael Josef
T1  - Development of a handheld sensor system for the online measurement of hydrogen peroxide in aseptic filling systems
JF  - Physica Status Solidi (A). 207 (2010), H. 4
Y1  - 2010
SN  - 1862-6300
N1  - Special Issue: Engineering of Functional Interfaces EnFI 2009
SP  - 913
EP  - 918
ER  - 
TY  - JOUR
A1  - Reisert, Steffen
A1  - Henkel, H.
A1  - Schneider, A.
A1  - Schäfer, D.
A1  - Friedrich, P.
A1  - Berger, J.
A1  - Schöning, Michael Josef
T1  - Entwicklung eines Handheld-Sensorsystems für die „On-line“-Messung der H2O2-Konzentration in aseptischen Entkeimungsprozessen
JF  - 9. Dresdner Sensor-Symposium : Dresden, 07.-09. Dezember 2009 / Gerlach, Gerald ; Hauptmann, Peter [Hrsg.]
Y1  - 2009
SN  - 978-3-941298-44-6
N1  - Dresdner Sensor-Symposium ; (9, 2009, Dresden)
SP  - 285
EP  - 288
PB  - TUDpress
CY  - Dresden
ER  - 
TY  - JOUR
A1  - Reisert, Steffen
A1  - Geissler, Hanno
A1  - Flörke, Rudolf
A1  - Weiler, Christian
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Characterisation of aseptic sterilisation processes using an electronic nose
JF  - International journal of nanotechnology
Y1  - 2013
SN  - 1475-7435 (Print) 7141-8151 (Online)
VL  - Vol. 10
IS  - No. 5-7
SP  - 470
EP  - 484
PB  - Inderscience Enterprises
CY  - Genève
ER  - 
TY  - JOUR
A1  - Reisert, Steffen
A1  - Geissler, Hanno
A1  - Flörke, Rudolf
A1  - Näther, Niko
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Towards a multi-sensor system for the evaluation of aseptic processes employing hydrogen peroxide vapour (H2O2)
JF  - Physica status solidi (a) : applications and material science. 208 (2011), H. 6
Y1  - 2011
SN  - 1862-6319
SP  - 1351
EP  - 1356
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Reisert, Steffen
A1  - Geissler, H.
A1  - Weiler, C.
A1  - Wagner, P.
A1  - Schöning, Michael Josef
T1  - Multiple sensor-type system for monitoring the microbicidal effectiveness of aseptic sterilisation processes
JF  - Food control
N2  - The present work describes a novel multiple sensor-type system for the real-time analysis of aseptic sterilisation processes employing gaseous hydrogen peroxide (H2O2) as a sterilant. The inactivation kinetics of Bacillus atrophaeus by gaseous H2O2 have been investigated by means of a methodical calibration experiment, taking into account the process variables H2O2 concentration, humidity and gas temperature. It has been found that the microbicidal effectiveness at H2O2 concentrations above 2% v/v is largely determined by the concentration itself, while at lower H2O2 concentrations, the gas temperature and humidity play a leading role. Furthermore, the responses of different types of gas sensors towards the influencing factors of the sterilisation process have been analysed within the same experiment. Based on a correlation established between the inactivation kinetics and the sensor responses, a calorimetric H2O2 sensor and a metal-oxide semiconductor (MOX) sensor have been identified as possible candidates for monitoring the microbicidal effectiveness of aseptic sterilisation processes employing gaseous H2O2. Therefore, two linear models that describe the relationship between sensor response and microbicidal effectiveness have been proposed.
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.foodcont.2014.07.063
SN  - 1873-7129 (E-Journal); 0956-7135 (Print)
VL  - 47
SP  - 615
EP  - 622
ER  - 
TY  - JOUR
A1  - Reisert, Steffen
A1  - Geissler, H.
A1  - Florke, R.
A1  - Wagner, P.
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
T1  - Controlling aseptic sterilization processes by means of a multi-sensor system
Y1  - 2011
N1  - 2011 IEEE Workshop on Merging Fields of Computational Intelligence and Sensor Technology ; 11.-15. April 2011 Paris, France
SP  - 18
EP  - 22
PB  - IEEE
CY  - New York
ER  - 
TY  - JOUR
A1  - Rachinger, Michael
A1  - Bauch, Melanie
A1  - Strittmatter, Axel
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Maurer, Karl-Heinz
A1  - Daniel, Rolf
A1  - Liebl, Wolfgang
A1  - Liesegang, Heiko
A1  - Ehrenreich, Armin
T1  - Size unlimited markerless deletions by a transconjugative plasmid-system in Bacillus licheniformis
JF  - Journal of biotechnology
Y1  - 2013
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - Vol. 164
IS  - Iss. 4
SP  - 365
EP  - 369
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Rabehi, Amine
A1  - Garlan, Benjamin
A1  - Achtsnicht, Stefan
A1  - Krause, Hans-Joachim
A1  - Offenhäusser, Andreas
A1  - Ngo, Kieu
A1  - Neveu, Sophie
A1  - Graff-Dubois, Stephanie
A1  - Kokabi, Hamid
T1  - Magnetic detection structure for Lab-on-Chip applications based on the frequency mixing technique
JF  - Sensors
N2  - A magnetic frequency mixing technique with a set of miniaturized planar coils was investigated for use with a completely integrated Lab-on-Chip (LoC) pathogen sensing system. The system allows the detection and quantification of superparamagnetic beads. Additionally, in terms of magnetic nanoparticle characterization ability, the system can be used for immunoassays using the beads as markers. Analytical calculations and simulations for both excitation and pick-up coils are presented; the goal was to investigate the miniaturization of simple and cost-effective planar spiral coils. Following these calculations, a Printed Circuit Board (PCB) prototype was designed, manufactured, and tested for limit of detection, linear response, and validation of theoretical concepts. Using the magnetic frequency mixing technique, a limit of detection of 15 µg/mL of 20 nm core-sized nanoparticles was achieved without any shielding.
KW  - Lab-on-Chip
KW  - magnetic sensing
KW  - frequency mixing
KW  - superparamagnetic nanoparticles
KW  - magnetic beads
Y1  - 2018
U6  - http://dx.doi.org/10.3390/s18061747
SN  - 1424-8220
VL  - 18
IS  - 6
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Pourshahidi, Ali Mohammad
A1  - Engelmann, Ulrich M.
A1  - Offenhäusser, Andreas
A1  - Krause, Hans-Joachim
T1  - Resolving ambiguities in core size determination of magnetic nanoparticles from magnetic frequency mixing data
JF  - Journal of Magnetism and Magnetic Materials
N2  - Frequency mixing magnetic detection (FMMD) has been widely utilized as a measurement technique in magnetic immunoassays. It can also be used for the characterization and distinction (also known as “colourization”) of different types of magnetic nanoparticles (MNPs) based on their core sizes. In a previous work, it was shown that the large particles contribute most of the FMMD signal. This leads to ambiguities in core size determination from fitting since the contribution of the small-sized particles is almost undetectable among the strong responses from the large ones. In this work, we report on how this ambiguity can be overcome by modelling the signal intensity using the Langevin model in thermodynamic equilibrium including a lognormal core size distribution fL(dc,d0,σ) fitted to experimentally measured FMMD data of immobilized MNPs. For each given median diameter d0, an ambiguous amount of best-fitting pairs of parameters distribution width σ and number of particles Np with R2 > 0.99 are extracted. By determining the samples’ total iron mass, mFe, with inductively coupled plasma optical emission spectrometry (ICP-OES), we are then able to identify the one specific best-fitting pair (σ, Np) one uniquely. With this additional externally measured parameter, we resolved the ambiguity in core size distribution and determined the parameters (d0, σ, Np) directly from FMMD measurements, allowing precise MNPs sample characterization.
Y1  - 2022
U6  - http://dx.doi.org/10.1016/j.jmmm.2022.169969
SN  - 0304-8853
VL  - 563
IS  - In progress, Art. No. 169969
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Pourshahidi, Ali Mohammad
A1  - Achtsnicht, Stefan
A1  - Offenhäusser, Andreas
A1  - Krause, Hans-Joachim
ED  - Offenhäusser, Andreas
T1  - Frequency Mixing Magnetic Detection Setup Employing Permanent Ring Magnets as a Static Offset Field Source
JF  - Sensors
N2  - Frequency mixing magnetic detection (FMMD) has been explored for its applications in fields of magnetic biosensing, multiplex detection of magnetic nanoparticles (MNP) and the determination of core size distribution of MNP samples. Such applications rely on the application of a static offset magnetic field, which is generated traditionally with an electromagnet. Such a setup requires a current source, as well as passive or active cooling strategies, which directly sets a limitation based on the portability aspect that is desired for point of care (POC) monitoring applications. In this work, a measurement head is introduced that involves the utilization of two ring-shaped permanent magnets to generate a static offset magnetic field. A steel cylinder in the ring bores homogenizes the field. By variation of the distance between the ring magnets and of the thickness of the steel cylinder, the magnitude of the magnetic field at the sample position can be adjusted. Furthermore, the measurement setup is compared to the electromagnet offset module based on measured signals and temperature behavior.
KW  - magnetic sensors
KW  - biosensors
KW  - frequency mixing magnetic detection
KW  - magnetic nanoparticles
Y1  - 2022
U6  - http://dx.doi.org/10.3390/s22228776
SN  - 1424-8220
VL  - 22
IS  - 22
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Pourshahidi, Ali Mohammad
A1  - Achtsnicht, Stefan
A1  - Nambipareechee, Mrinal Murali
A1  - Offenhäusser, Andreas
A1  - Krause, Hans-Joachim
T1  - Multiplex detection of magnetic beads using offset field dependent frequency mixing magnetic detection
JF  - Sensors
N2  - Magnetic immunoassays employing Frequency Mixing Magnetic Detection (FMMD) have recently become increasingly popular for quantitative detection of various analytes. Simultaneous analysis of a sample for two or more targets is desirable in order to reduce the sample amount, save consumables, and save time. We show that different types of magnetic beads can be distinguished according to their frequency mixing response to a two-frequency magnetic excitation at different static magnetic offset fields. We recorded the offset field dependent FMMD response of two different particle types at frequencies ƒ₁ + n⋅ƒ₂, n = 1, 2, 3, 4 with ƒ₁ = 30.8 kHz and ƒ₂ = 63 Hz. Their signals were clearly distinguishable by the locations of the extremes and zeros of their responses. Binary mixtures of the two particle types were prepared with different mixing ratios. The mixture samples were analyzed by determining the best linear combination of the two pure constituents that best resembled the measured signals of the mixtures. Using a quadratic programming algorithm, the mixing ratios could be determined with an accuracy of greater than 14%. If each particle type is functionalized with a different antibody, multiplex detection of two different analytes becomes feasible.
KW  - colorization
KW  - multiplex detection
KW  - frequency mixing magnetic detection
KW  - magnetic nanoparticles
Y1  - 2021
U6  - http://dx.doi.org/10.3390/s21175859
SN  - 1424-8220
N1  - This article belongs to the Special Issue "Advanced Nanomaterial-Based Sensors for Biomedical Applications"
VL  - 21
IS  - 17
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Polen, T.
A1  - Krämer, Marco
A1  - Bongaerts, Johannes
A1  - Wubbolts, Marcel
A1  - Wendisch, V. F.
T1  - The global gene expression response of Escherichia coli to L-phenylalanine
JF  - Journal of biotechnology
Y1  - 2005
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - Vol. 115
IS  - Iss. 3
SP  - 221
EP  - 237
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Yoshinobu, Tatsuo
A1  - Simonis, A.
A1  - Ecken, H.
A1  - Lüth, Hans
A1  - Schöning, Michael Josef
T1  - Penicillin detection by means of field-effect based sensors: EnFET, capacitive EIS sensor or LAPS?
JF  - Sensors and Actuators B. 78 (2001), H. 1-3
Y1  - 2001
SN  - 0925-4005
SP  - 237
EP  - 242
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Yoshinobu, Tatsuo
A1  - Schöning, Michael Josef
T1  - Flow-velocity microsensors based on semiconductor field-effect structures
JF  - Sensors. 3 (2003), H. 7
Y1  - 2003
SN  - 1424-8220
SP  - 202
EP  - 212
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Yoshinobu, T.
A1  - Simonis, A.
A1  - Ecken, H.
A1  - Lüth, H.
A1  - Schöning, Michael Josef
T1  - Penicillin detection by means of field-effect based sensors: EnFET, capacitive EIS sensor or LAPS?
JF  - Proceedings : Copenhagen, Denmark, 27 - 30 August 2000 / [ed.: R. de Reus ...]
Y1  - 2000
SN  - 87-89935-50-0
N1  - Eurosensors ; (14, 2000, København) ; Eurosensors ; (14 : ; 2000.08.27-30 : ; Copenhagen) ; European Conference on Solid-State Transducers ; (14 : ; 2000.08.27-30 : ; Copenhagen)
SP  - 27
EP  - 30
PB  - MIC, Mikroelektronik Centret
CY  - Lyngby, Denmark
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Werner, Frederik
A1  - Buniatyan, V. V.
A1  - Wagner, Torsten
A1  - Miamoto, K.
A1  - Yoshinobu, T.
A1  - Schöning, Michael Josef
T1  - Towards addressability of light-addressable potentiometric sensors: Shunting effect of non-illuminated region and cross-talk
JF  - Sensor and Actuators B: Chemical
N2  - The LAPS (light-addressable potentiometric sensor) platform is one of the most attractive approaches for chemical and biological sensing with many applications ranging from pH and ion/analyte concentration measurements up to cell metabolism detection and chemical imaging. However, although it is generally accepted that LAPS measurements are spatially resolved, the light-addressability feature of LAPS devices has not been discussed in detail so far. In this work, an extended electrical equivalent-circuit model of the LAPS has been presented, which takes into account possible cross-talk effects due to the capacitive coupling of the non-illuminated region. A shunting effect of the non-illuminated area on the measured photocurrent and addressability of LAPS devices has been studied. It has been shown, that the measured photocurrent will be determined not only by the local interfacial potential in the illuminated region but also by possible interfacial potential changes in the non-illuminated region, yielding cross-talk effects. These findings were supported by the experimental investigations of a penicillin-sensitive multi-spot LAPS and a metal-insulator-semiconductor LAPS as model systems.
Y1  - 2017
U6  - http://dx.doi.org/10.1016/j.snb.2017.01.047
SN  - 0925-4005
IS  - 244
SP  - 1071
EP  - 1079
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Welden, Rene
A1  - Buniatyan, Vahe V.
A1  - Schöning, Michael Josef
T1  - An Array of On-Chip Integrated, Individually Addressable Capacitive Field-Effect Sensors with Control Gate: Design and Modelling
JF  - Sensors
N2  - The on-chip integration of multiple biochemical sensors based on field-effect electrolyte-insulator-semiconductor capacitors (EISCAP) is challenging due to technological difficulties in realization of electrically isolated EISCAPs on the same Si chip. In this work, we present a new simple design for an array of on-chip integrated, individually electrically addressable EISCAPs with an additional control gate (CG-EISCAP). The existence of the CG enables an addressable activation or deactivation of on-chip integrated individual CG-EISCAPs by simple electrical switching the CG of each sensor in various setups, and makes the new design capable for multianalyte detection without cross-talk effects between the sensors in the array. The new designed CG-EISCAP chip was modelled in so-called floating/short-circuited and floating/capacitively-coupled setups, and the corresponding electrical equivalent circuits were developed. In addition, the capacitance-voltage curves of the CG-EISCAP chip in different setups were simulated and compared with that of a single EISCAP sensor. Moreover, the sensitivity of the CG-EISCAP chip to surface potential changes induced by biochemical reactions was simulated and an impact of different parameters, such as gate voltage, insulator thickness and doping concentration in Si, on the sensitivity has been discussed.
KW  - equivalent circuit
KW  - multianalyte detection
KW  - control gate
KW  - on-chip integrated addressable EISCAP sensors
KW  - capacitive field-effect sensor
Y1  - 2021
U6  - http://dx.doi.org/10.3390/s21186161
SN  - 1424-8220
N1  - This article belongs to the Special Issue "Field-Effect Sensors: From pH Sensing to Biosensing"
VL  - 21
IS  - 18
SP  - 17
PB  - MDPI
CY  - Basel
ER  -