TY  - JOUR
A1  - Welden, Melanie
A1  - Poghossian, Arshak
A1  - Vahidpour, Farnoosh
A1  - Wendlandt, Tim
A1  - Keusgen, Michael
A1  - Wege, Christina
A1  - Schöning, Michael Josef
T1  - Towards multi-analyte detection with field-effect capacitors modified with tobacco mosaic virus bioparticles as enzyme nanocarriers
JF  - Biosensors
N2  - Utilizing an appropriate enzyme immobilization strategy is crucial for designing enzyme-based biosensors. Plant virus-like particles represent ideal nanoscaffolds for an extremely dense and precise immobilization of enzymes, due to their regular shape, high surface-to-volume ratio and high density of surface binding sites. In the present work, tobacco mosaic virus (TMV) particles were applied for the co-immobilization of penicillinase and urease onto the gate surface of a field-effect electrolyte-insulator-semiconductor capacitor (EISCAP) with a p-Si-SiO₂-Ta₂O₅ layer structure for the sequential detection of penicillin and urea. The TMV-assisted bi-enzyme EISCAP biosensor exhibited a high urea and penicillin sensitivity of 54 and 85 mV/dec, respectively, in the concentration range of 0.1–3 mM. For comparison, the characteristics of single-enzyme EISCAP biosensors modified with TMV particles immobilized with either penicillinase or urease were also investigated. The surface morphology of the TMV-modified Ta₂O₅-gate was analyzed by scanning electron microscopy. Additionally, the bi-enzyme EISCAP was applied to mimic an XOR (Exclusive OR) enzyme logic gate.
KW  - urease
KW  - enzyme-logic gate
KW  - bi-enzyme biosensor
KW  - capacitive field-effect sensor
KW  - tobacco mosaic virus (TMV)
KW  - penicillinase
Y1  - 2022
U6  - http://dx.doi.org/10.3390/bios12010043
SN  - 2079-6374
N1  - This article belongs to the Special Issue "Biosensors: 10th Anniversary Feature Papers"
VL  - 12
IS  - 1
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Wardoyo, Arinto Y.P.
A1  - Noor, Johan A.E.
A1  - Elbers, Gereon
A1  - Schmitz, Sandra
A1  - Flaig, Sascha T.
A1  - Budianto, Arif
T1  - Characterizing volcanic ash elements from the 2015 eruptions of bromo and raung volcanoes, Indonesia
JF  - Polish Journal of Environmental Studies
N2  - The volcanic eruptions of Mt. Bromo and Mt. Raung in East Java, Indonesia, in 2015 perturbed volcanic materials and affected surface-layer air quality at surrounding locations. During the episodes, the volcanic ash from the eruptions influenced visibility, traffic accidents, flight schedules, and human health. In this research, the volcanic ash particles were collected and characterized by relying on the detail of physical observation. We performed an assessment of the volcanic ash elements to characterize the volcanic ash using two different methods which are aqua regia extracts followed by MP-AES and XRF laboratory test of bulk samples. The analysis results showed that the volcanic ash was mixed of many materials, such as Al, Si, P, K, Ca, Ti, V, Cr, Mn, Fe, Ni, and others. Fe, Si, Ca, and Al were found as the major elements, while the others were the trace elements Ba, Cr, Cu, Mn, P, Mn, Ni, Zn, Sb, Sr, and V with the minor concentrations. XRF analyses showed that Fe dominated the elements of the volcanic ash. The XRF analysis showed that Fe was at 35.40% in Bromo and 43.00% in Raung of the detected elements in bulk material. The results of aqua regia extracts analyzed by MP-AES were 1.80% and 1.70% of Fe element for Bromo and Raung volcanoes, respectively.
Y1  - 2020
U6  - http://dx.doi.org/10.15244/pjoes/99101
SN  - 2083-5906
VL  - 29
IS  - 2
SP  - 1899
EP  - 1907
PB  - HARD
CY  - Olsztyn
ER  - 
TY  - JOUR
A1  - Wang, Ren-Qi
A1  - Druckenmüller, Katharina
A1  - Elbers, Gereon
A1  - Guenther, Klaus
A1  - Croué, Jean-Philippe
T1  - Analysis of aquatic-phase natural organic matter by optimized LDI-MS method
JF  - Journal of mass spectrometry
N2  - The composition and physiochemical properties of aquatic-phase natural organic matter (NOM) are most important problems for both environmental studies and water industry. Laser desorption/ionization (LDI) mass spectrometry facilitated successful examinations of NOM, as humic and fulvic acids in NOM are readily ionized by the nitrogen laser. In this study, hydrophobic NOMs (HPO NOMs) from river, reservoir and waste water were characterized by this technique. The effect of analytical variables like concentration, solvent composition and laser energy was investigated. The exact masses of small molecular NOM moieties in the range of 200–1200 m/z were determined in reflectron mode. In addition, spectra of post-source-decay experiments in this range showed that some compounds from different natural NOMs had the same fragmental ions. In the large mass range of 1200–15 000 Da, macromolecules and their aggregates were found in HPO NOMs from natural waters. Highly humic HPO exhibited mass peaks larger than 8000 Da. On the other hand, the waste water and reservoir water mainly had relatively smaller molecules of about 2000 Da. The LDI-MS measurements indicated that highly humic river waters were able to form large aggregates and membrane foulants, while the HPO NOMs from waste water and reservoir water were unlikely to form large aggregates. Copyright © 2014 John Wiley & Sons, Ltd.
Y1  - 2014
U6  - http://dx.doi.org/10.1002/jms.3321
SN  - 1096-9888
VL  - 49
IS  - 2
SP  - 154
EP  - 160
PB  - Wiley
CY  - Bognor Regis
ER  - 
TY  - JOUR
A1  - Waller, Mark P.
A1  - Braun, Heiko
A1  - Hojdis, Nils
A1  - Bühl, Michael
T1  - Geometries of Second-Row Transition-Metal Complexes from Density-Functional Theory
JF  - Journal of Chemical Theory and Computation
Y1  - 2007
U6  - http://dx.doi.org/10.1021/ct700178y
SN  - 1549-9626
VL  - 3
IS  - 6
SP  - 2234
EP  - 2242
ER  - 
TY  - JOUR
A1  - Wagner, Torsten
A1  - Molina, Roberto
A1  - Yoshinobu, Tatsuo
A1  - Kloock, Joachim P.
A1  - Biselli, Manfred
A1  - Canzoneri, Michele
A1  - Schnitzler, Thomas
A1  - Schöning, Michael Josef
T1  - Handheld multi-channel LAPS device as a transducer platform for possible biological and chemical multi-sensor applications
JF  - Electrochimica Acta. 53 (2007), H. 2
Y1  - 2007
SN  - 0013-4686
SP  - 305
EP  - 311
ER  - 
TY  - JOUR
A1  - Wagner, Torsten
A1  - Molina, R.
A1  - Biselli, Manfred
A1  - Canzoneri, Michele
A1  - Schnitzler, Thomas
A1  - Yoshinobu, Tatsuo
A1  - Schöning, Michael Josef
T1  - A light-addressable potentiometric sensor system for fast, simultaneous and spatial detection of the metabolic activity of biological cells
JF  - Transducers '07 Eurosensors XXI : digest of technical papers ; the14th International Conference on Solid-State Sensors, Actuators and Microsystems, June 10-14, 2007, Lyon, France / Gilles Delapierre (Ed.)
Y1  - 2007
SN  - 1-4244-0841-5
N1  - Eurosensors 21, 2007, Lyon ; International Conference on Solid-State Sensors, Actuators and Microsystems 14, 2007, Lyon
SP  - 1107
EP  - 1110
PB  - IEEE
CY  - Piscataway
ER  - 
TY  - JOUR
A1  - Wagner, Torsten
A1  - Beging, Stefan
A1  - Rotter, L.
A1  - Poghossian, Arshak
A1  - Biselli, Manfred
A1  - Zang, Werner
A1  - Schöning, Michael Josef
T1  - Online-Messsysteme für die automatisierte Charakterisierung von feldeffektbasierten Biosensoren
JF  - 8. Dresdner Sensor-Symposium : Sensoren für Umwelt, Klima und Sicherheit, Biosensoren und Biosysteme, Sensoren und Sensorsysteme für die Prozesstechnik, Trends in der Sensortechnik, Materialentwicklung für die Sensorik; 8. Dresdner Sensor-Symposium, 10. - 12. Dezember 2007, Dresden / Gerald Gerlach ... (Hg.)
Y1  - 2007
SN  - 978-3-940046-45-1
N1  - Dresdner Sensor-Symposium <8, 2007, Dresden> ; Dresdner Beiträge zur Sensorik ; 29
SP  - 257
EP  - 260
PB  - TUDpress, Verl. der Wissenschaften
CY  - Dresden
ER  - 
TY  - CHAP
A1  - Wagemann, Kurt
A1  - Tippkötter, Nils
T1  - Biorefineries: a short introduction
T2  - Biorefineries
N2  - The terms bioeconomy and biorefineries are used for a variety of processes and developments. This short introduction is intended to provide a delimitation and clarification of the terminology as well as a classification of current biorefinery concepts. The basic process diagrams of the most important biorefinery types are shown.
KW  - Bioeconomy
KW  - Biorefinery definitions
KW  - Introduction
KW  - Process schemes
KW  - Renewable resources
Y1  - 2019
SN  - 978-3-319-97117-9
SN  - 978-3-319-97119-3
U6  - http://dx.doi.org/10.1007/10_2017_4
N1  - (Advances in Biochemical Engineering/Biotechnology book series ; Vol. 166)
SP  - 1
EP  - 11
PB  - Springer
CY  - Cham
ER  - 
TY  - BOOK
A1  - Wagemann, Kurt
A1  - Tippkötter, Nils
T1  - Biorefineries / Kurt Wagemann, Nils Tippkötter (editors)
T3  - Advances in biochemical engineering/biotechnology book series (ABE)
Y1  - 2019
SN  - 978-3-319-97117-9
SN  - 978-3-319-97119-3
U6  - http://dx.doi.org/10.1007/978-3-319-97119-3
PB  - Springer
CY  - Cham (Switzerland)
ER  - 
TY  - JOUR
A1  - Wackwitz, B.
A1  - Bongaerts, Johannes
A1  - Goodman, S. D.
A1  - Unden, Gottfried
T1  - Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significance
JF  - Molecular and general genetics : MGG
Y1  - 1999
SN  - 1617-4623 (E-Journal); 1617-4615 (Print)
VL  - Vol. 262
IS  - Iss. 4 - 5
SP  - 876
EP  - 883
ER  - 
TY  - JOUR
A1  - Voigt, Birgit
A1  - Schroeter, Rebecca
A1  - Jürgen, Britta
A1  - Albrecht, Dirk
A1  - Evers, Stefan
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - Schweder, Thomas
A1  - Hecker, Michael
T1  - The response of Bacillus licheniformis to heat and ethanol stress and the role of the SigB regulon
JF  - Proteomics
Y1  - 2013
SN  - 1615-9861 (E-Journal); 1615-9853 (Print)
VL  - Vol. 13
IS  - Iss. 14
SP  - 2140
EP  - 2146
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Voigt, Birgit
A1  - Albrecht, Dirk
A1  - Sievers, Susanne
A1  - Becher, Dörte
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Schweder, Thomas
A1  - Maurer, Karl-Heinz
A1  - Hecker, Michael
T1  - High-resolution proteome maps of Bacillus licheniformis cells growing in minimal medium
JF  - Proteomics
Y1  - 2015
U6  - http://dx.doi.org/10.1002/pmic.201400504
SN  - 1615-9861
VL  - 15
IS  - 15
SP  - 2629
EP  - 2633
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - RPRT
A1  - Vaeßen, Christiane
A1  - Ohme, H.
A1  - Manderscheid, D.
T1  - Endbericht Projekt Immotherm : Vorhabensbezeichnung: KMU-innovativ-Verbundvorhaben "Einsatz immobilisierter Mikroorganismen zur Entölung und Entsalzung von Kondensatwasser bei hohen Prozesstemperaturen" : Laufzeit des Vorhabens: 01.03.2009-31.08.2011 : Förderkennzeichen:  01LY0816A, 01LY0816B, 01LY0816C
Y1  - 2012
ER  - 
TY  - JOUR
A1  - Unden, Gottfried
A1  - Bongaerts, Johannes
T1  - Alternative respiratory pathways of Escherichia coli: energetics and transcriptional regulation in response to electron acceptors
JF  - Biochimica et biophysica acta (BBA) - Bioenergetics
Y1  - 1997
SN  - 1879-2650 (E-Journal); 0005-2728 (Print)
VL  - Vol. 1320
IS  - Iss. 3
SP  - 217
EP  - 234
ER  - 
TY  - JOUR
A1  - Unden, G.
A1  - Becker, S.
A1  - Bongaerts, Johannes
A1  - Schirawski, J.
A1  - Six, S.
T1  - Oxygen regulated gene expression in facultatively anaerobic bacteria
JF  - Antonie van Leeuwenhoek
Y1  - 1994
SN  - 0003-6072 (Print) ; 1572-9699 (online)
VL  - Vol. 66
IS  - Iss. 1-3
SP  - 3
EP  - 22
ER  - 
TY  - JOUR
A1  - Unden, G.
A1  - Becker, S.
A1  - Bongaerts, Johannes
A1  - Holighaus, G.
A1  - Schirawski, J.
A1  - Six, S.
T1  - O2-sensing and O2-dependent gene regulation in facultatively anaerobic bacteria
JF  - Archives of microbiology
Y1  - 1995
SN  - 1432-072X (E-Journal); 0003-9276 (Print); 0302-8933 (Print)
VL  - Vol. 164
IS  - Iss. 2
SP  - 81
EP  - 90
ER  - 
TY  - JOUR
A1  - Ulber, Roland
A1  - Tippkötter, Nils
T1  - Nitratfreie Molke
JF  - Rundschau für Fleischhygiene und Lebensmittelüberwachung
Y1  - 2009
IS  - 4
SP  - 150
EP  - 152
ER  - 
TY  - JOUR
A1  - Ulber, Roland
A1  - Poth, Sebastian
A1  - Monzon, Magaly
A1  - Tippkötter, Nils
T1  - Prozessintegration von Hydrolyse und Fermentation von Cellulose- Faserstoff
JF  - Chemie Ingenieur Technik
N2  - Ein viel versprechender erneuerbarer Rohstoff für die Produktion von Chemikalien und Treibstoffen ist Lignocellulose aus pflanzlicher Biomasse. Die darin enthaltenen Zucker können mittels enzymatischer Hydrolyse freigesetzt und fermentativ zu Ethanol umgesetzt werden. Ein interessanter Ansatz ist dabei die simultane Verzuckerung und Fermentation. Hefen und Enzyme haben mit 30 °C bzw. 50 °C zwar unterschiedliche Temperaturoptima, es konnte aber gezeigt werden, dass auch bei den niedrigeren Temperaturen eine Umsetzung der Cellulose zu Glucose erfolgt, wenn auch langsamer als bei optimalen Bedingungen. Außerdem konnte in Vorversuchen gezeigt werden, dass Ethanol in den zu erwartenden Konzentrationen keinen Einfluss auf die enzymatische Umsetzung hat.
Y1  - 2010
U6  - http://dx.doi.org/10.1002/cite.200900103
SN  - 1522-2640
N1  - Special Issue "Biokatalyse"
VL  - 82
IS  - 1-2
SP  - 135
EP  - 139
ER  - 
TY  - CHAP
A1  - Ulber, Roland
A1  - Muffler, Kai
A1  - Tippkötter, Nils
A1  - Hirth, Thomas
A1  - Sell, Dieter
ED  - Ulber, Roland
ED  - Sell, Dieter
ED  - Hirth, Thomas
T1  - Introduction to Renewable Resources in the Chemical Industry
T2  - Renewable raw materials : new feedstocks for the chemical industry
Y1  - 2011
SN  - 978-3-527-32548-1
SP  - 1
EP  - 6
PB  - Wiley-VCH-Verlag
CY  - Weinheim
ET  - 1. Auflage
ER  - 
TY  - JOUR
A1  - Ulber, R.
A1  - Tippkötter, Nils
A1  - Buchholz, H.
A1  - Demmer, W.
A1  - Scheper, T.
T1  - Innovative Verfahren in der Molkeaufarbeitung zur Gewinnung neuer Produkte
JF  - Deutsche Milchwirtschaft
Y1  - 2008
SN  - 0012-0480
VL  - 59
IS  - 19
SP  - 704
EP  - 706
ER  - 
TY  - JOUR
A1  - Tüg, Helmut
A1  - Baumann, Marcus
T1  - Problems of UV-B radiation measurements in biological research : critical remarks on current techniques and suggestions for improvements
JF  - Geophysical research letters
Y1  - 1994
SN  - 1944-8007 (E-Journal); 0094-8276 (Print)
VL  - Vol. 21
IS  - Iss. 8
SP  - 689
EP  - 692
ER  - 
TY  - JOUR
A1  - Tüg, Helmut
A1  - Baumann, Marcus
T1  - Reply to the comments by R.L. McKenzie and P.V. Johnston on our paper “Problems of UV-B radiation measurements in biological research: Critical Remarks on current techniques and suggestions for improvements”
JF  - Geophysical research letters
Y1  - 1995
SN  - 1944-8007 (E-Journal); 0094-8276 (Print)
VL  - Vol. 22
IS  - Iss. 9
SP  - 1159
EP  - 1160
ER  - 
TY  - JOUR
A1  - Turek, Monika
A1  - Ketterer, Lothar
A1  - Claßen, Melanie
A1  - Berndt, Heinz
A1  - Elbers, Gereon
A1  - Krüger, Peter
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Development and Electrochemical Investigations of an EIS-(Electrolyte-Insulator-Semiconductor) based Biosensor for Cyanide Detection
JF  - Sensors
Y1  - 2007
SN  - 1424-8220
VL  - 7
IS  - 8
SP  - 1415
EP  - 1426
ER  - 
TY  - JOUR
A1  - Turck, Christoph W.
A1  - Berndt, Heinz
T1  - Synthese definierter Peptid-Derivate durch Aminolyse von 3-(Nα-Acyl-peptidyloxy)-2-hydroxy-N-alkylbenzamiden bei erhöhten Temperaturen, I : Synthese des Modellpeptid-Derivates Z-Ala-Phe-Gly-N(Et)2
JF  - Hoppe-Seyler´s Zeitschrift für physiologische Chemie
Y1  - 1981
U6  - http://dx.doi.org/10.1515/bchm2.1981.362.1.821
SN  - 1437-4315
SN  - 0018-4888
VL  - 362
IS  - 1
SP  - 821
EP  - 828
ER  - 
TY  - JOUR
A1  - Trapp, Svenja
A1  - Lammers, Tom
A1  - Engudar, Gokce
A1  - Hoehr, Cornelia
A1  - Denkova, Antonia G.
A1  - Paulßen, Elisabeth
A1  - de Kruijff, Robin M.
T1  - Membrane-based microfluidic solvent extraction of Ga-68 from aqueous Zn solutions: towards an automated cyclotron production loop
JF  - EJNMMI Radiopharmacy and Chemistry
KW  - Microfluidic solvent extraction
KW  - Ga-68
KW  - Cyclotron production
KW  - Medical radionuclide production
KW  - Metal contaminants
Y1  - 2023
U6  - http://dx.doi.org/10.1186/s41181-023-00195-2
SN  - 2365-421X
VL  - 2023
IS  - 8, Article number: 9
SP  - 1
EP  - 14
PB  - Springer Nature
ER  - 
TY  - JOUR
A1  - Tran, Quang Hon
A1  - Bongaerts, Johannes
A1  - Vlad, Dorina
A1  - Unden, Gottfried
T1  - Requirement for the proton-pumping NADH dehydrogenase I of Escherichia coli in respiration of NADH to fumarate and its bioenergetic implications
JF  - European journal of biochemistry
Y1  - 1997
SN  - 0014-2956
VL  - Vol. 244
IS  - Iss. 1
SP  - 155
EP  - 160
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Wollny, Steffen
A1  - Suck, Kirstin
A1  - Sohling, Ulrich
A1  - Ruf, Friedrich
A1  - Ulber, Roland
T1  - Recycling of spent oil bleaching earth as source of glycerol for the anaerobic production of acetone, butanol, and ethanol with Clostridium diolis and lipolytic Clostridium lundense
JF  - Engineering in Life Sciences
N2  - A major part of edible oil is subjected to bleaching procedures, primarily with minerals applied as adsorbers. Their recycling is currently done either by regaining the oil via organic solvent extraction or by using the spent bleaching earth (SBE) as additive for animal feed, etc. As a new method, the reutilization of the by-product SBE for the microbiologic formation of acetone, butanol, and ethanol (ABE) is presented as proof-of-concept. The SBE was taken from a palm oil cleaning process. The recycling concept is based on the application of lipolytic clostridia strains. Due to considerably long fermentation times, co-fermentation with Candida rugosa and enzymatic hydrolyses of the bound oil with a subsequent clostridia fermentation are shown as alternative routes. Anaerobic fermentations under comparison of different clostridia strains were performed with glycerol media, enzymatically hydrolyzed palm oil and SBE. Solutes, side product compositions and productivities were quantified via HPLC. A successful production of ABE solutes from SBE has been done with a yield of 0.15 g butanol per gram of bound glycerol. Thus, the biotechnological recycling of the waste stream is possible in principle. Inhibition of the substrate suspension has been observed. A chromatographic ion-exchange of substrates increased the biomass concentration.
Y1  - 2014
U6  - http://dx.doi.org/10.1002/elsc.201300113
SN  - 1618-2863
VL  - 14
IS  - 4
SP  - 425
EP  - 432
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Wollny, S.
A1  - Kampeis, P.
A1  - Oster, J.
A1  - Schneider, H.
A1  - Ulber, R.
T1  - Magnetseparation von Proteinen : Separation von Zielmolekülen durch hochselektive Aptamere
JF  - GIT Labor-Fachzeitschrift
N2  - Durch die Kombination von Oligonukleotid-Liganden (Aptameren) hoher Bindungsaffinitäten
mit hochselektiv abtrennbaren magnetisierbaren Mikropartikeln
wird eine einstufige Separation von Zielmolekülen aus mikrobiologischen
Produktionsansätzen möglich. Die Aptamere werden hierfür reversibel
auf den Partikeloberflächen gebunden und für die spezifische Isolierung von
Bioprodukten eingesetzt. Die Abtrennung der beladenen Partikel erfolgt
durch einen neuen Rotor-Stator-Separator mit Hochgradient-Magnetfeld.
Y1  - 2011
VL  - 55
IS  - 10
SP  - 666
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - RPRT
A1  - Tippkötter, Nils
A1  - Wagner, Sebastian
T1  - Biomimetischer Klebstoff aus ligninhaltigen Pflanzenresten (Teilvorhaben 1 und 2) : Schlussbericht zum Vorhaben : Laufzeit: 01.01.2016 bis 31.03.2019
Y1  - 2019
U6  - http://dx.doi.org/10.2314/KXP:169732777X
N1  - Förderkennzeichen BMEL 22030514 - 22004615
PB  - FH Aachen
CY  - Jülich
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Stückmann, Henning
A1  - Kroll, Stephen
A1  - Winkelmann, Gunda
A1  - Noack, Udo
A1  - Scheper, Thomas
A1  - Ulber, Roland
T1  - A semi-quantitative dipstick assay for microcystin
JF  - Analytical and Bioanalytical Chemistry
N2  - An immunochromatographic lateral flow dipstick assay for the fast detection of microcystin-LR was developed. Colloid gold particles with diameters of 40 nm were used as red-colored antibody labels for the visual detection of the antigen. The new dipstick sensor is capable of detecting down to 5 µg·l−1 (ppb; total inversion of the color signal) or 1 ppb (observation of color grading) of microcystin-LR. The course of the labeling reaction was observed via spectrometric wave shifts caused by the change of particle size during the binding of antibodies. Different stabilizing reagents showed that especially bovine serum albumin (BSA) and casein increase the assays sensitivity and the conjugate stability. Performance of the dipsticks was quantified by pattern processing of capture zone CCD images. Storage stability of dipsticks and conjugate suspensions over 115 days under different conditions were monitored. The ready-to-use dipsticks were successfully tested with microcystin-LR-spiked samples of outdoor drinking- and salt water and applied to the tissue of microcystin-fed mussels.
Y1  - 2009
U6  - http://dx.doi.org/10.1007/s00216-009-2750-8
SN  - 1618-2650
VL  - 394
IS  - 3
SP  - 863
EP  - 869
PB  - springer
CY  - Berlin
ER  - 
TY  - CHAP
A1  - Tippkötter, Nils
A1  - Stückmann, H.
A1  - Winkelmann, G.
A1  - Noack, U.
A1  - Beutel, S.
A1  - Scheper, T.
A1  - Ulber, R.
T1  - Optimisation of antibody-labelling of gold colloids for their application in an immunchromatographic assay for microcystin-LR
T2  - European BioPerspectives : celebrating the 25th DECHEMA annual convention of biotechnologists ; 30 May - 1 June 2007, Cologne, Germany ; book of abstracts ; abstracts, poster programme
Y1  - 2007
SP  - 126
PB  - Dechema
CY  - Frankfurt am Main
ER  - 
TY  - CHAP
A1  - Tippkötter, Nils
A1  - Schünemann, V.
A1  - Christmann, R.
A1  - Pasteur, A.
A1  - Schweizer, J.
A1  - Ulber, R.
ED  - Beckmann, Dieter
T1  - Bioaffinity Layering magnetisierbarer Mikro- und Nanopartikel
T2  - Technische Systeme für die Lebenswissenschaften : 14. Heiligenstädter Kolloquium, Heilbad Heiligenstadt, 22.09. - 24.09.2008
Y1  - 2008
SN  - 978-3-00-025695-0
SP  - 97
EP  - 98
CY  - Heiligenstadt
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Roth, Jasmine
T1  - Purified Butanol from Lignocellulose – Solvent‐Impregnated Resins for an Integrated Selective Removal
JF  - Chemie Ingenieur Technik
N2  - In traditional microbial biobutanol production, the solvent must be recovered during fermentation process for a sufficient space-time yield. Thermal separation is not feasible due to the boiling point of n-butanol. As an integrated and selective solid-liquid separation alternative, solvent impregnated resins (SIRs) were applied. Two polymeric resins were evaluated and an extractant screening was conducted. Vacuum application with vapor collection in fixed-bed column as bioreactor bypass was successfully implemented as butanol desorption step. In course of further increasing process economics, fermentation with renewable lignocellulosic substrates was conducted using Clostridium acetobutylicum. Utilization of SIR was shown to be a potential strategy for solvent removal from fermentation broth, while application of a bypass column allows for product removal and recovery at once.
KW  - Biofuel
KW  - Biorefinery
KW  - Butanol
KW  - Clostridium acetobutylicum
KW  - Lignocellulose
Y1  - 2020
U6  - http://dx.doi.org/10.1002/cite.202000200
SN  - 1522-2640
VL  - 92
IS  - 11
SP  - 1741
EP  - 1751
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Roikaew, Wipa
A1  - Ulber, Roland
A1  - Hoffmann, Alexander
A1  - Denzler, Hans-Jörg
A1  - Buchholz, Heinrich
T1  - Paracoccus denitrificans for the effluent recycling during continuous denitrification of liquid food
JF  - Biotechnology Progress
N2  - Nitrate is an undesirable component of several foods. A typical case of contamination with high nitrate contents is whey concentrate, containing nitrate in concentrations up to 25 l. The microbiological removal of nitrate by Paracoccus denitrificans under formation of harmless nitrogen in combination with a cell retention reactor is described here. Focus lies on the resource-conserving design of a microbal denitrification process. Two methods are compared. The application of polyvinyl alcohol-immobilized cells, which can be applied several times in whey feed, is compared with the implementation of a two step denitrification system. First, the whey concentrate's nitrate is removed by ion exchange and subsequently the eluent regenerated by microorganisms under their retention by crossflow filtration. Nitrite and nitrate concentrations were determined by reflectometric color measurement with a commercially available Reflectoquant® device. Correction factors for these media had to be determined. During the pilot development, bioreactors from 4 to 250 mg·L-1 and crossflow units with membrane areas from 0.02 to 0.80 m2 were examined. Based on the results of the pilot plants, a scaling for the exemplary process of denitrifying 1,000 tons per day is discussed.
Y1  - 2010
U6  - http://dx.doi.org/10.1002/btpr.384
SN  - 8756-7938
VL  - 26
IS  - 3
SP  - 756
EP  - 762
PB  - Wiley
CY  - Hoboken, NJ
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Roikaew, W.
A1  - Ulber, R.
T1  - Nitrate removal from whey concentrate with biotechnological regeneration of the waste water
JF  - European dairy magazine : EDM
Y1  - 2008
SN  - 0936-6318
IS  - 1
SP  - 30
EP  - 32
ER  - 
TY  - CHAP
A1  - Tippkötter, Nils
A1  - Roikaew, W.
A1  - Ulber, R.
T1  - An automated pilot plant for the bioengineering processing of concentrated whey
T2  - European BioPerspectives : in cooperation with BIOTECHNICA 2008 : 7 - 9 October 2008 Hannover, Germany ; book of abstracts ; abstracts, poster programme
Y1  - 2008
SP  - 98
PB  - Dechema
CY  - Frankfurt am Main
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Roikaew, N.
A1  - Ulber, R.
T1  - Nitratentfernung aus Molkekonzentrat mit biotechnologischer Regeneration der Abwässer
JF  - Deutsche Milchwirtschaft
Y1  - 2007
SN  - 0012-0480
VL  - 58
IS  - 15
SP  - 540
EP  - 542
ER  - 
TY  - CHAP
A1  - Tippkötter, Nils
A1  - Möhring, Sophie
A1  - Roth, Jasmine
A1  - Wulfhorst, Helene
T1  - Logistics of lignocellulosic feedstocks: preprocessing as a preferable option
T2  - Biorefineries
N2  - In comparison to crude oil, biorefinery raw materials are challenging in concerns of transport and storage. The plant raw materials are more voluminous, so that shredding and compacting usually are necessary before transport. These mechanical processes can have a negative influence on the subsequent biotechnological processing and shelf life of the raw materials. Various approaches and their effects on renewable raw materials are shown. In addition, aspects of decentralized pretreatment steps are discussed. Another important aspect of pretreatment is the varying composition of the raw materials depending on the growth conditions. This problem can be solved with advanced on-site spectrometric analysis of the material.
KW  - Analytics
KW  - Decentral
KW  - Mechanical
KW  - On-site
KW  - Pre-treatment
Y1  - 2019
SN  - 978-3-319-97117-9
SN  - 978-3-319-97119-3
U6  - http://dx.doi.org/10.1007/10_2017_58
N1  - Advances in biochemical engineering/biotechnology ; Vol. 166
SP  - 43
EP  - 68
PB  - Springer
CY  - Cham
ER  - 
TY  - CHAP
A1  - Tippkötter, Nils
A1  - Möhring, S.
A1  - Maurer, S.
A1  - Roth, J.
T1  - Dezentrale Vorbehandlung und Verarbeitung pflanzlicher Reststoffe für Bioraffinerien
T2  - Kurzfassungen der Vorträge nach Sessions : Frühjahrstagung der Biotechnologen 2013, 4. - 5. März 2013, Dechema-Haus, Frankfurt am Main
Y1  - 2013
SP  - 5
CY  - Frankfurt am Main
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Duwe, Anna-Maria
A1  - Wiesen, Sebastian
A1  - Sieker, Tim
A1  - Ulber, Roland
T1  - Enzymatic hydrolysis of beech wood lignocellulose at high solid contents and its utilization as substrate for the production of biobutanol and dicarboxylic acids
JF  - Bioresource Technology
N2  - The development of a cost-effective hydrolysis for crude cellulose is an essential part of biorefinery developments. To establish such high solid hydrolysis, a new solid state reactor with static mixing is used. However, concentrations >10% (w/w) cause a rate and yield reduction of enzymatic hydrolysis. By optimizing the synergetic activity of cellulolytic enzymes at solid concentrations of 9%, 17% and 23% (w/w) of crude Organosolv cellulose, glucose concentrations of 57, 113 and 152 g L⁻¹ are reached. However, the glucose yield decreases from 0.81 to 0.72gg⁻¹ at 17% (w/w). Optimal conditions for hydrolysis scale-up under minimal enzyme addition are identified. As result, at 23% (w/w) crude cellulose the glucose yield increases from 0.29 to 0.49gg⁻¹. As proof of its applicability, biobutanol, succinic and itaconic acid are produced with the crude hydrolysate. The potential of the substrate is proven e.g. by a high butanol yield of 0.33gg⁻¹.
Y1  - 2014
U6  - http://dx.doi.org/10.1016/j.biortech.2014.06.052
VL  - 167
SP  - 447
EP  - 455
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Deterding, A.
A1  - Ulber, Roland
T1  - Determination of acetic acid in fermentation broth by gas-diffusion technique
JF  - Engineering in Life Sciences
N2  - Due to the interfering effects of acetic acid in many fermentation processes, a gas-diffusion technique was developed for the online determination of acetic acid. The measurements were accomplished with a flow diffusion analysis (FDA) unit from the TRACE Analytics GmbH, Braunschweig, Germany. The diffusion analysis is based on the UV-absorbance of acetic acid at 205 nm. The measurement was achieved by the separation of an acceptor and a carrier stream (acidified fermentation broth) using a gas permeable polytetrafluoroethylene (PTFE) membrane, whereby broth constituents that would otherwise disturb the UV-measurement of acetic acid, are held back efficiently. Merely, the fermentation by-products, e.g. formic acid, is capable of diffusing through the membrane. While formic acid can disturb the measurement, carbon dioxide does not absorb at 205 nm. The method operates with time-dependent sample enrichment. During the analysis, a small volume of the acceptor stream is stopped for a defined time interval in the acceptor chamber. During this period, the gaseous acetic acid diffuses through the membrane and is enriched in the acceptor chamber. Subsequently after the enrichment, the acceptor stream flows through a UV-detector. The intensity of the signal is proportional to the acetic acid concentration. Online measurements in bioreactors via a sterile filtration probe have been accomplished. A linear calibration in the range of 0.5–5.0 g/L acetic acid with a relative standard deviation of <5 % was obtained. A sampling rate of 8 samples per hour was possible. The system was applied for the determination of acetic acid in E. coli fermentation broth. The instrument is easy to clean, very user-friendly and does not require any toxic or expensive reagents.
Y1  - 2008
U6  - http://dx.doi.org/10.1002/elsc.200820227
VL  - 8
IS  - 1, Special Issue: Technical Systems for the Use in Life Sciences
SP  - 62
EP  - 67
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Al-Kaidy, Huschyar
A1  - Wollny, Steffen
A1  - Ulber, Roland
T1  - Functionalized magnetizable particles for downstream processing in single-use systems
JF  - Chemie Ingenieur Technik
N2  - Biotechnological downstream processing is usually an elaborate procedure, requiring a multitude of unit operations to isolate the target component. Besides the disadvantageous space-time yield, the risks of cross-contaminations and product loss grow fast with the complexity of the isolation procedure. A significant reduction of unit operations can be achieved by application of magnetic particles, especially if these are functionalized with affinity ligands. As magnetic susceptible materials are highly uncommon in biotechnological processes, target binding and selective separation of such particles from fermentation or reactions broths can be done in a single step. Since the magnetizable particles can be produced from iron salts and low priced polymers, a single-use implementation of these systems is highly conceivable. In this article, the principles of magnetizable particles, their synthesis and functionalization are explained. Furthermore, applications in the area of reaction engineering, microfluidics and downstream processing are discussed focusing on established single-use technologies and development potential.
Y1  - 2013
U6  - http://dx.doi.org/10.1002/cite.201200130
VL  - 85
IS  - 1-2: Special Issue: Single-Use Technology
SP  - 76
EP  - 86
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - CHAP
A1  - Tippkötter, Nils
ED  - Kaltschmidt, Martin
T1  - Grundlagen der bio-chemischen Umwandlung
T2  - Energie aus Biomasse : Grundlagen, Techniken und Verfahren
Y1  - 2016
SN  - 978-3-662-47437-2 (Print)
SN  - 978-3-662-47438-9 (Online)
U6  - http://dx.doi.org/10.1007/978-3-662-47438-9
SP  - 1447
EP  - 1500
PB  - Springer Vieweg
CY  - Berlin ; Heidelberg
ET  - 3., aktualisierte, erweiterte Auflage
ER  - 
TY  - BOOK
A1  - Tippkötter, Nils
T1  - Reaktionssysteme zur Aufarbeitung und Umsetzung nachwachsender Rohstoffe : Einsatz chromatographischer Verfahren sowie Membran- und Festbettreaktoren zur Verarbeitung von Molke, Stärke und Cellulose
Y1  - 2010
SN  - 978-3-8325-2717-4
N1  - Kaiserslautern, Technische Universität, Dissertation, 2010
PB  - Logos-Verlag
CY  - Berlin
ER  - 
TY  - CHAP
A1  - Tippkötter, Nils
T1  - Biotechnologische Gewinnung von Wertstoffen aus Molke : BiobasedWorld - Innovation in food
T2  - Biotechnica 2013 : European biotechnology science & industry news
Y1  - 2013
VL  - 12
IS  - 9, special
SP  - 33
EP  - 50
ER  - 
TY  - RPRT
A1  - Tippkötter, Nils
T1  - Lokale Vorbehandlung nachwachsender Rohstoffe für Bioraffinerien (BioSats) : Schlussbericht zum Vorhaben : Laufzeit: 01.03.2012 bis 30.04.2017
Y1  - 2018
U6  - http://dx.doi.org/10.2314/GBV:1024204243
ER  - 
TY  - JOUR
A1  - Thiel, Alexander
A1  - Tippkötter, Nils
A1  - Suck, Kirstin
A1  - Sohling, Ulrich
A1  - Ruf, Friedrich
A1  - Ulber, Roland
T1  - New zeolite adsorbents for downstream processing of polyphenols from renewable resources
JF  - Engineering in Life Sciences
N2  - Commercial materials with polyvinylpolypyrrolidone and polymeric amberlites (XAD7HP, XAD16) are commonly used for the adsorptive downstream processing of polyphenols from renewable resources. In this study, beta-zeolite-based adsorbent systems were examined, and their properties were compared to organic resins. Batch adsorption experiments were conducted with synthetic solutions of major polyphenols. Adsorption isotherms and desorption characteristics of individual adsorbent were determined based on these results. Maximum adsorption capacities were calculated using the Langmuir model. For example, the zeolites had capacities up to 203.2 mg/g for ferulic acid. To extend these results to a complex system, additional experiments were performed on rapeseed meal and wheat seed extracts as representative renewable resources. HPLC analysis showed that with 7.5% w/v, which is regarded as the optimum amount of zeolites, zeolites A and B could bind 100% of the major polyphenols as well as release polyphenols at high yields. Additionally, regeneration experiments were performed with isopropyl alcohol at 99°C to evaluate how zeolites regenerate under mild conditions. The results showed only a negligible loss of adsorption capacity and no loss of desorption capacity. In summary, it was concluded that beta-zeolites were promising adsorbents for developing new processes to isolate polyphenols from renewable resources.
Y1  - 2013
U6  - http://dx.doi.org/10.1002/elsc.201200188
VL  - 13
IS  - 3
SP  - 239
EP  - 246
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Thiel, Alexander
A1  - Muffler, Kai
A1  - Tippkötter, Nils
A1  - Suck, Kirstin
A1  - Sohling, Ulrich
A1  - Ruf, Friedrich
A1  - Ulber, Roland
T1  - Aufarbeitung von Polyphenolen aus Weizen mittels Zeolithen am Beispiel der Ferulasäure
JF  - Chemie IngenieurTechnik
N2  - Aufarbeitung von Polyphenolen aus Weizenmittels Zeolithen am Beispiel der Ferulasa¨ ureAlexander Thiel1, Kai Muffler1, Nils Tippko¨ tter1, Kirstin Suck2, Ulrich Sohling2, Friedrich Ruf3und Roland Ulber1,*DOI: 10.1002/cite.201400031Bei der Ferulasa¨ure handelt es sich um einen Wertstoff, der aus Weizen gewonnen und in der Lebensmittel- und Pharma-industrie eingesetzt werden kann. Der Einsatz von Weizen als nachwachsende Rohstoffquelle ist allerdings nur dann wirt-schaftlich durchfu¨hrbar, wenn eine Prozessintegration in die bestehenden industriellen Verfahren gewa¨hrleistet oder einedirekte Konkurrenz zur Mehl- und Sta¨rkeindustrie vermieden werden kann. In diesem Artikel wird ein Verfahren aufge-zeigt, welches hohe Ausbeuten ermo¨glicht und eine Konkurrenz zu bestehenden Verwertungspfaden vermeidet.
Y1  - 2015
U6  - http://dx.doi.org/10.1002/cite.201400031
N1  - Englischer Titel: Downstream Processing of Polyphenols from Wheat by Zeolites using the Example of Ferulic Acid
VL  - 87
IS  - 1-2
SP  - 128
EP  - 136
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Thiel, Alexander
A1  - Muffler, Kai
A1  - Tippkötter, Nils
A1  - Suck, Kirstin
A1  - Sohling, Ulrich
A1  - Hruschka, Steffen M.
A1  - Ulber, Roland
T1  - A novel integrated downstream processing approach to recover sinapic acid, phytic acid and proteins from rapeseed meal
JF  - Journal of Chemical Technology and Biotechnology
N2  - BACKGROUND
Currently, several techniques exist for the downstream processing of protein, phytic acid and sinapic acid from rapeseed and rapeseed meal, but no technique has been developed to separate all of the components in one process. In this work, two new downstream processing strategies focusing on recovering sinapic acid, phytic acid and protein from rapeseed meal were established.

RESULTS
The sinapic acid content was enhanced by a factor of 4.5 with one method and 5.1 with the other. The isolation of sinapic acid was accomplished using a zeolite-based adsorbent with high adsorptive and optimal desorption characteristics. Phytic acid was isolated using the anion-exchange resin Purolite A200®. In addition, the processes resulted in two separated protein fractions. The ratios of globulin and albumin ratio to the total protein were 59.2% and 40.1%, respectively. The steps were then combined in two different ways: (a) a ‘sequential process’ using the zeolite and A200 in batch processes; and (b) a ‘parallel process’ using only A200 in a chromatographic system to separate all of the compounds.

CONCLUSIONS
It can be concluded that isolation of all three components was possible in both processes. These could enhance the added value of current processes using rapeseed meal as a protein source. © 2015 Society of Chemical Industry
Y1  - 2015
U6  - http://dx.doi.org/10.1002/jctb.4664
VL  - 90
IS  - 11
SP  - 1999
EP  - 2006
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Teumer, T.
A1  - Capitain, C.
A1  - Ross-Jones, J.
A1  - Tippkötter, Nils
A1  - Rädle, M.
A1  - Methner, F.-J.
T1  - In-line Haze Monitoring Using a Spectrally Resolved Back Scattering Sensor
JF  - BrewingScience
N2  - In the present work an optical sensor in combination with a spectrally resolved detection device for in-line particle-size-monitoring for quality control in beer production is presented. The principle relies on the size and wavelength dependent backscatter of growing particles in fluids. Measured interference structures of backscattered light are compared with calculated theoretical values, based on Mie-Theory, and fitted with a linear least square method to obtain particle size distributions. For this purpose, a broadband light source in combination with a process-CCD-spectrometer (charge ? coupled device spectrometer) and process adapted fiber optics are used. The goal is the development of an easy and flexible measurement device for in-line-monitoring of particle size. The presented device can be directly installed in product fill tubes or vessels, follows CIP- (cleaning in place) and removes the need of sample taking. A proof of concept and preliminary results, measuring protein precipitation, are presented.
Y1  - 2018
SN  - 1613-2041
VL  - 71
IS  - 5/6
SP  - 49
EP  - 55
PB  - Fachverlag Hans Carl
CY  - Nürnberg
ER  - 
TY  - THES
A1  - Temiz Artmann, Aysegül
T1  - Sicanlarda egzersiz sonrasi oksidatif hasar ve eritrosit membran degisikliklerinin hemoreolojik etkileri
T1  - The effect of oxidative stress and erythrocyte membrane changes on hemorheological factors following exercise in rats
Y1  - 2001
N1  - Dokuz Eylül Üniversitesi, Izmir, Diss., 2001. 
Veröffentlicht unter Temiz, Aysegül
ER  - 
TY  - THES
A1  - Temiz Artmann, Aysegül
T1  - Sicanlarda, akut egzersiz sonucu gelisen oksidan stresin lökosit aktivasyon degisiklikleri ile ile olan iliskisi ve eritrosit deformabilitesine etkisi
T1  - The oxidative stress effect on leukocyte activation and erythrocyte deformability after acute exercise in rats
Y1  - 1996
N1  - Dokuz Eylül Üniversitesi, Izmir, Diss., 1996.
Veröffentlicht unter Temiz, Ayşegül
ER  - 
TY  - JOUR
A1  - Takenaga, Shoko
A1  - Schneider, Benno
A1  - Erbay, E.
A1  - Biselli, Manfred
A1  - Schnitzler, Thomas
A1  - Schöning, Michael Josef
A1  - Wagner, Torsten
T1  - Fabrication of biocompatible lab-on-chip devices for biomedical applications by means of a 3D-printing process
JF  - Physica status solidi (a)
N2  - A new microfluidic assembly method for semiconductor-based biosensors using 3D-printing technologies was proposed for a rapid and cost-efficient design of new sensor systems. The microfluidic unit is designed and printed by a 3D-printer in just a few hours and assembled on a light-addressable potentiometric sensor (LAPS) chip using a photo resin. The cell growth curves obtained from culturing cells within microfluidics-based LAPS systems were compared with cell growth curves in cell culture flasks to examine biocompatibility of the 3D-printed chips. Furthermore, an optimal cell culturing within microfluidics-based LAPS chips was achieved by adjusting the fetal calf serum concentrations of the cell culture medium, an important factor for the cell proliferation.
Y1  - 2015
U6  - http://dx.doi.org/10.1002/pssa.201532053
SN  - 1862-6319
VL  - 212
IS  - 6
SP  - 1347
EP  - 1352
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - CHAP
A1  - Takenaga, Shoko
A1  - Herrera, Cony F.
A1  - Werner, Frederik
A1  - Biselli, Manfred
A1  - Schnitzler, Thomas
A1  - Schöning, Michael Josef
A1  - Öhlschläger, Peter
A1  - Wagner, Torsten
T1  - Detection of the metabolic activity of cells by differential measurements based on a single light-addressable potentiometric sensor chip
T2  - 11. Dresdner Sensor-Symposium : 9.-11.12.2013
Y1  - 2013
SN  - 978-3-9813484-5-3
SP  - 63
EP  - 67
ER  - 
TY  - JOUR
A1  - Takenaga, Shoko
A1  - Biselli, Manfred
A1  - Schnitzler, Thomas
A1  - Öhlschläger, Peter
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
T1  - Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis–Menten-like kinetics for cell culturing
JF  - Physica status solidi A : Applications and materials science
N2  - The metabolic activity of Chinese hamster ovary (CHO) cells was observed using a light-addressable potentiometric sensor (LAPS). The dependency toward different glucose concentrations (17–200 mM) follows a Michaelis–Menten kinetics trajectory with Kₘ = 32.8 mM, and the obtained Kₘ value in this experiment was compared with that found in literature. In addition, the pH shift induced by glucose metabolism of tumor cells transfected with the HPV-16 genome (C3 cells) was successfully observed. These results indicate the possibility to determine the tumor cells metabolism with a LAPS-based measurement device.
Y1  - 2014
U6  - http://dx.doi.org/10.1002/pssa.201330464
SN  - 1521-396X (E); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print)
VL  - 211
IS  - 6
SP  - 1410
EP  - 1415
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Svaneborg, Carsten
A1  - Karimi-Varzaneh, Hossein Ali
A1  - Hojdis, Nils
A1  - Fleck, Franz
A1  - Everaers, Ralf
T1  - Multiscale approach to equilibrating model polymer melts
JF  - Physical Review E
N2  - We present an effective and simple multiscale method for equilibrating Kremer Grest model polymer melts of varying stiffness. In our approach, we progressively equilibrate the melt structure above the tube scale, inside the tube and finally at the monomeric scale. We make use of models designed to be computationally effective at each scale. Density fluctuations in the melt structure above the tube scale are minimized through a Monte Carlo simulated annealing of a lattice polymer model. Subsequently the melt structure below the tube scale is equilibrated via the Rouse dynamics of a force-capped Kremer-Grest model that allows chains to partially interpenetrate. Finally the Kremer-Grest force field is introduced to freeze the topological state and enforce correct monomer packing. We generate 15 melts of 500 chains of 10.000 beads for varying chain stiffness as well as a number of melts with 1.000 chains of 15.000 monomers. To validate the equilibration process we study the time evolution of bulk, collective, and single-chain observables at the monomeric, mesoscopic, and macroscopic length scales. Extension of the present method to longer, branched, or polydisperse chains, and/or larger system sizes is straightforward.
Y1  - 2016
U6  - http://dx.doi.org/10.1103/PhysRevE.94.032502
SN  - 2470-0053
VL  - 94
IS  - 032502
PB  - AIP Publishing
CY  - Melville, NY
ER  - 
TY  - JOUR
A1  - Svaneborg, Carsten
A1  - Karimi-Varzaneh, Hossein Ali
A1  - Hojdis, Nils
A1  - Fleck, Franz
A1  - Everaers, Ralf
T1  - Kremer-Grest Models for Universal Properties of Specific Common Polymer Species
JF  - Soft Condensed Matter
N2  - The Kremer-Grest (KG) bead-spring model is a near standard in Molecular Dynamic simulations of generic polymer properties. It owes its popularity to its computational efficiency, rather than its ability to represent specific polymer species and conditions. Here we investigate how to adapt the model to match the universal properties of a wide range of chemical polymers species. For this purpose we vary a single parameter originally introduced by Faller and Müller-Plathe, the chain stiffness. Examples include polystyrene, polyethylene, polypropylene, cis-polyisoprene, polydimethylsiloxane, polyethyleneoxide and styrene-butadiene rubber. We do this by matching the number of Kuhn segments per chain and the number of Kuhn segments per cubic Kuhn volume for the polymer species and for the Kremer-Grest model. We also derive mapping relations for converting KG model units back to physical units, in particular we obtain the entanglement time for the KG model as function of stiffness allowing for a time mapping. To test these relations, we generate large equilibrated well entangled polymer melts, and measure the entanglement moduli using a static primitive-path analysis of the entangled melt structure as well as by simulations of step-strain deformation of the model melts. The obtained moduli for our model polymer melts are in good agreement with the experimentally expected moduli.
Y1  - 2018
IS  - 1606.05008
ER  - 
TY  - PAT
A1  - Stellberg, Michael
A1  - Jeromin, Günter Erich
T1  - Rührvorrichtung für Magnetrührsystem : Patentschrift DE19807518C2 ; Veröffentlichungstag der Patenterteilung: 31.05.2000
Y1  - 2000
N1  - Volltext über Datenbank DEPATISnet
PB  - Deutsches Patent- und Markenamt
CY  - München
ER  - 
TY  - JOUR
A1  - Stanley, Lesley A.
A1  - Horsburgh, Brian C.
A1  - Ross, Jillian
A1  - Scheer, Nico
A1  - Wolf, C. Roland
T1  - Drug transporters: Gatekeepers controlling access of xenobiotics to the cellular interior
JF  - Drug Metabolism Reviews
Y1  - 2009
U6  - http://dx.doi.org/10.1080/03602530802605040
SN  - 1097-9883
VL  - 41
IS  - 1
SP  - 27
EP  - 65
PB  - Taylor & Francis
CY  - London
ER  - 
TY  - JOUR
A1  - Stanley, Lesley A.
A1  - Horsburgh, Brian C.
A1  - Ross, Jillian
A1  - Scheer, Nico
A1  - Wolf, C. Roland
T1  - Nuclear Receptors which play a pivotal role in drug disposition and chemical toxicity
JF  - Drug Metabolism Reviews
Y1  - 2006
U6  - http://dx.doi.org/10.1080/03602530600786232
SN  - 1097-9883
VL  - 38
IS  - 3
SP  - 515
EP  - 597
ER  - 
TY  - PAT
A1  - Stadtmüller, Ralf
A1  - Tippkötter, Nils
A1  - Ulber, Roland
T1  - A method for production of single-stranded nucleic acids [Europäische Patentanmeldung]
Y1  - 2013
PB  - Europäisches Patentamt
CY  - Den Hague
ER  - 
TY  - PAT
A1  - Stadtmüller, Ralf
A1  - Tippkötter, Nils
A1  - Ulber, Roland
T1  - Method for production of single-stranded macronucleotides
N2  - The invention relates to a method for production of single-stranded macronucleotides by amplifying and ligating an extended monomeric single-stranded target nucleic acid sequence (targetss) into a repetitive cluster of double-stranded target nucleic acid sequences (targetds), and subsequently cloning the construct into a vector (aptagene vector). The aptagene vector is transformed into host cells for replication of the aptagene and isolated in order to optain single-stranded target sequences (targetss). The invention also relates to single-stranded nucleic acids, produced by a method of the invention.
Y1  - 2015
N1  - Patent auch unter EP2774996, EP2774996, US2017145460 und US9944966 veröffentlicht.
ER  - 
TY  - CHAP
A1  - Srivastava, Alok
A1  - Knolle, Friedhart
A1  - Hoyler, Friedrich
A1  - Scherer, Ulrich W.
A1  - Schnug, Ewald
T1  - Uranium Toxicity in the State of Punjab in North-Western India
T2  - Management of Natural Resources in a Changing Environment
N2  - Lately there has been an increasing concern about uranium toxicity in some districts of Punjab State located in the North Western part of India after the publication of a report (Blaurock-Busch et al. 2010) which showed that the concentration of uranium in hair and urine of children suffering from physical deformities, neurological and mental disorder from Malwa region (Fig. 1) of Punjab State was manifold higher than the reference ranges. A train which connects the affected region with the nearby city of Bikaner which has a Cancer Hospital has been nicknamed as Cancer Express due to the frenzy generated on account of uranium related toxicity.
Y1  - 2015
SN  - 978-3-319-12559-6
U6  - http://dx.doi.org/10.1007/978-3-319-12559-6_21
SP  - 271
EP  - 275
PB  - Springer
CY  - Cham
ER  - 
TY  - CHAP
A1  - Srivastava, Alok
A1  - Knolle, F.
A1  - Schnug, E.
A1  - Scherer, Ulrich W.
T1  - Study of Trace Elements in Water Bodies of the Harz Mts. Region, Germany using Total Reflection X Ray Fluorescence (TXRF)
T2  - DAE-BRNS 12th National Symposium on Nuclear and Radiochemistry NUCAR 2015, Feb. 9-13, Mumbai, India, Mumbai, India; 02/2015
Y1  - 2015
SP  - 355
EP  - 356
ER  - 
TY  - JOUR
A1  - Srivastava, A.
A1  - Singh, V.
A1  - Aggarwal, P.
A1  - Schneeweiss, F.
A1  - Scherer, Ulrich W.
A1  - Friedrich, W.
T1  - Optical studies of insulating polymers for radiation dose monitoring
JF  - Indian Journal of Pure & Applied Physics
Y1  - 2010
SN  - 0019-5596
N1  - Special Issue: SI
VL  - 48
IS  - 11
SP  - 782
EP  - 786
ER  - 
TY  - JOUR
A1  - Spiess, Elmar
A1  - Wilfried, Reichardt
A1  - Alvarez, Gerardo
A1  - Gottrup, Marcus
A1  - Öhlschläger, Peter
T1  - An Artificial PAP Gene Breaks Self-tolerance and Promotes Tumor Regression in the TRAMP Model for Prostate Carcinoma
JF  - Molecular Therapy
Y1  - 2011
SN  - 1525-0016
VL  - 20
IS  - 3
SP  - 555
EP  - 564
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Smith, Walker O.
A1  - Baumann, Marcus
A1  - Wilson, David L.
A1  - Aletsee, Ludwig
T1  - Phytoplankton biomass and productivity in the marginal ice zone of the Fram Strait during summer 1984
JF  - Journal of geophysical research
Y1  - 1987
SN  - 2156-2202 (E-Journal); 2169-9291 (E-Journal); 0148-0227 (Print); 2169-9275 (Print)
VL  - Vol. 92
IS  - Iss. C7
SP  - 6777
EP  - 6786
ER  - 
TY  - JOUR
A1  - Sieker, Tim
A1  - Ulber, Roland
A1  - Dimitrova, Darina
A1  - Bart, Hans-Jörg
A1  - Neuner, Andreas
A1  - Heinzle, Elmar
A1  - Tippkötter, Nils
T1  - Silage : Fermentationsrohstoff für die chemische Industrie?
JF  - labor&more
N2  - In Anbetracht des zu erwartenden Rückgangs der Verfügbarkeit fossiler Rohstoffe müssen nicht nur für den Energiesektor, sondern auch für die Herstellung industrieller Produkte alternative Rohstoffe gefunden werden. Ein Beispiel für einen nicht in Nahrungsmittelkonkurrenz stehenden nachwachsenden Rohstoff ist grüne Biomasse wie Gras und Klee. Diese lassen sich in Deutschland auf großen Flächen anbauen und enthalten eine Vielzahl potenzieller Substrate für Fermentationen.
Y1  - 2009
IS  - 2
SP  - 44
EP  - 45
ER  - 
TY  - JOUR
A1  - Sieker, Tim
A1  - Neuner, Andreas
A1  - Dimitrova, Darina
A1  - Tippkötter, Nils
A1  - Muffler, Kai
A1  - Bart, Hans-Jörg
A1  - Heinzle, Elmar
A1  - Ulber, Roland
T1  - Ethanol production from grass silage by simultaneous pretreatment, saccharification and fermentation: First steps in the process development
JF  - Engineering in Life Sciences
N2  - Grass silage provides a great potential as renewable feedstock. Two fractions of the grass silage, a press juice and the fiber fraction, were evaluated for their possible use for bioethanol production. Direct production of ethanol from press juice is not possible due to high concentrations of organic acids. For the fiber fraction, alkaline peroxide or enzymatic pretreatment was used, which removes the phenolic acids in the cell wall. In this study, we demonstrate the possibility to integrate the enzymatic pretreatment with a simultaneous saccharification and fermentation to achieve ethanol production from grass silage in a one-process step. Achieved yields were about 53 g ethanol per kg silage with the alkaline peroxide pretreatment and 91 g/kg with the enzymatic pretreatment at concentrations of 8.5 and 14.6 g/L, respectively. Furthermore, it was shown that additional supplementation of the fermentation medium with vitamins, trace elements and nutrient salts is not necessary when the press juice is directly used in the fermentation step.
Y1  - 2011
U6  - http://dx.doi.org/10.1002/elsc.201000160
N1  - Special Issue "Bioprocess‐oriented plant design"
VL  - 11
IS  - 4
SP  - 436
EP  - 442
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Sieker, Tim
A1  - Neuner, Andreas
A1  - Dimitrova, Darina
A1  - Tippkötter, Nils
A1  - Bart, Hans-Jörg
A1  - Heinzle, Elmar
A1  - Ulber, Roland
T1  - Grassilage als Rohstoff für die chemische Industrie
JF  - Chemie Ingenieur Technik
N2  - Grassilage stellt einen nachwachsenden Rohstoff mit großem Potenzial dar. Neben Cellulose und Hemicellulose enthält sie auch organische Säuren, insbesondere Milchsäure. In einem Bioraffinerie-Projekt wird die Milchsäure aus der Silage isoliert und mit gentechnisch optimierten Stämmen zu L-Lysin weiterverarbeitet. Die Lignocellulose wird hydrolysiert und zu Ethanol fermentiert. Ein besonderes Augenmerk liegt auf der Integration der unterschiedlichen Prozesse sowie der einzelnen Prozessschritte zu einem Gesamtprozess, der sämtliche Inhaltsstoffe der Silage verwertet.
Y1  - 2010
U6  - http://dx.doi.org/10.1002/cite.201000088
SN  - 1522-2640
VL  - 82
IS  - 8, Special Issue: Industrielle Nutzung nachwachsender Rohstoffe
SP  - 1153
EP  - 1159
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - CHAP
A1  - Sieker, T.
A1  - Duwe, A.
A1  - Poth, S.
A1  - Tippkötter, Nils
A1  - Ulber, R.
T1  - Herstellung von Itaconsäure aus Buchenholzhydrolysaten
T2  - Kurzfassungsband / GVC-DECHEMA Vortrags- und Diskussionstagung Biopharmazeutische Produktion : 14. - 16. Mai 2012. Konzerthaus Freibung
Y1  - 2012
SP  - 57
PB  - DECHEMA
CY  - Frankfurt, M.
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Wieland, Susanne
A1  - Engelskirchen, Julia
A1  - Merkel, Marion
A1  - Maurer, Karl-Heinz
A1  - Bessler, Cornelius
T1  - Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease [Offenlegungsschrift]
T1  - Novel alkaline protease from Bacillus Gibsonii and washing and cleaning agents containing said novel alkaline protease [Europäische Patentanmeldung / US Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2008
SP  - 1
EP  - 51
PB  - Deutsches Patentamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Spitz, Astrid
A1  - Maurer, Karl-Heinz
T1  - Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]
T1  - Novel proteases and compositions comprising these proteases [Internationale Patentanmeldung]
Y1  - 2010
SP  - 1
EP  - 31
PB  - Deutsches Patentamt / WIPO
CY  - München / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Spitz, Astrid
A1  - Maurer, Karl-Heinz
T1  - Wasch- und Reinigungsmittel enthaltend Proteasen aus Bacillus pumilus [Offenlegungsschrift]
T1  - Detergents and cleaning agents containing proteases from bacillus pumilus [Europäische Patentschrift / Internationale Patentanmeldung]
Y1  - 2010
SP  - 1
EP  - 20
PB  - Deutsches Patentamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Schwaneberg, Ulrich
A1  - Martinez Moya, Ronny
A1  - Merkel, Marion
A1  - Spitz, Astrid
A1  - Wieland, Susanne
A1  - Hellmuth, Hendrik
A1  - Maurer, Karl-Heinz
T1  - Leistungsverbesserte Proteasevariante [Offenlegungsschrift]
T1  - Performance-enhanced protease variant [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2012
SP  - 1
EP  - 29
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - CHAP
A1  - Siegert, Petra
A1  - Pohl, Martina
A1  - Kneen, Malea M.
A1  - Pogozheva, Irina D.
A1  - Kenyon, George L.
A1  - McLeish, Michael J.
T1  - Exploring the substrate specificity of benzoylformate decarboxylase, pyruvate decarboxylase, and benzaldehyde lyase
T2  - Thiamine : catalytic mechanisms in normal and disease states / ed. by Frank Jordan ...
Y1  - 2004
SN  - 0-8247-4062-9
SP  - 275
EP  - 290
PB  - Dekker
CY  - New York, NY
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Mussmann, Nina
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
T1  - Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]
T1  - Novel proteases and means containing said proteases [Internationale Patentanmeldung]
Y1  - 2010
SP  - 1
EP  - 30
PB  - Deutsches Patent- und Markenamt / WIPO
CY  - München / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Merkel, Marion
A1  - Kluin, Cornelia
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
T1  - Wasch- und Reinigungsmittel enthaltend Proteasen aus Xanthomonas [Offenlegungsschrift]
T1  - Detergents and cleaners comprising proteases from xanthomonas [Internationale Patentanmeldung]
Y1  - 2008
SP  - 1
EP  - 15
PB  - Deutsches Patent- und Markenamt / WIPO
CY  - München / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Merkel, Marion
A1  - Kluin, Cornelia
A1  - Maurer, Karl-Heinz
A1  - O'Connell, Timothy
A1  - Wieland, Susanne
A1  - Hellmuth, Hendrik
T1  - Neue Proteasen und diese enthaltende Mittel [Offenlegungsschrift]
T1  - Novel proteases and compositions comprising them [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2011
SP  - 1
EP  - 21
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Merkel, Marion
A1  - Hellmuth, Hendrik
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
T1  - Stabilisierte flüssige enzymhaltige Tensidzubereitung (Einsatz einer das hydrolytische Enzym stabilisierenden Komponente, die eine Phenylalkyldicarbonsäure umfasst) [Offenlegungsschrift]
T1  - Stabilized liquid tenside preparation comprising enzymes (using a component that stabilizes the hydrolytic enzyme and includes a phenylalkyldicarboxylic acid) [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2012
SP  - 1
EP  - 15
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Merkel, Marion
A1  - Hellmuth, Hendrik
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
T1  - Stabilisierte flüssige enzymhaltige Tensidzubereitung (durch den Einsatz einer das hydrolytische Enzym stabilisierende Komponente, die eine mehrfach substituierte Benzolcarbonsäure umfasst, die an mindestens zwei Kohlenstoffatomen des Benzolrestes eine Carboxylgruppe aufweist) [Offenlegungsschrift]
T1  - Stabilized liquid tenside preparation comprising enzymes (a component comprising a multiply substituted benzyl carboxylic acid comprising a carboxyl group on at least two carbon atoms of the benzyl radical is used for stabilizing the hydrolytic enzyme) [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2012
SP  - 1
EP  - 16
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Merkel, Marion
A1  - Hellmuth, Hendrik
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
T1  - Stabilisierte flüssige enzymhaltige Tensidzubereitung (Einsatz einer das hydrolytische Enzym stabilisierende Komponente, die eine Phthaloylglutaminsäure und/oder eine Phthaloylasparaginsäure umfasst) [Offenlegungsschrift]
T1  - Stabilized liquid tenside preparation comprising enzymes (a component comprising a phthaloyl glutamine acid and/or a phthaloyl asparagine acid is used for stablizing the hydrolytic enzyme) [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2012
SP  - 1
EP  - 16
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Merkel, Marion
A1  - Hellmuth, Hendrik
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
T1  - Stabilisierte flüssige enzymhaltige Tensidzubereitung (Einsatz einer das hydrolytische Enzym stabilisierende Komponente, die eine Aminophthalsäure umfasst) [Offenlegungsschrift]
T1  - Stabilized liquid tenside preparation comprising enzymes (a component comprising an aminophthalic acid is used for stabilizing the hydrolytic enzyme) [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2012
SP  - 1
EP  - 16
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Merkel, Marion
A1  - Hellmuth, Hendrik
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
T1  - Stabilisierte flüssige enzymhaltige Tensidzubereitung (Einsatz einer das hydrolytische Enzym stabilisierende Komponente, die eine Oligoamino-biphenyl-oligocarbonsäure umfasst) [Offenlegungsschrift]
T1  - Stabilized liquid tenside preparation comprising enzymes (a component comprising an oligoamino-biphenyl-oligocarboxylic acid is used for stabilizing the hydrolytic enzyme) [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2012
SP  - 1
EP  - 16
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Merkel, Marion
A1  - Hellmuth, Hendrik
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
T1  - Stabilisierte flüssige enzymhaltige Tensidzubereitung (Einsatz einer das hydrolytische Enzym stabilisierende Komponente, die ein Monosaccharidglycerat umfasst) [Offenlegungsschrift]
T1  - Stabilized liquid enzyme-containing surfactant preparation (using a component which comprises a monosaccharide glycerate and which stabilizes the hydrolytic enzyme) [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2012
SP  - 1
EP  - 17
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - JOUR
A1  - Siegert, Petra
A1  - McLeish, Michael J.
A1  - Baumann, Martin
A1  - Iding, Hans
A1  - Kneen, Malea M.
A1  - Kenyon, George L.
A1  - Pohl, Martina
T1  - Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida
JF  - Protein engineering, design, and selection : peds
Y1  - 2005
SN  - 1460-213X (E-Journal); 1741-0134 (E-Journal); 0269-2139 (Print); 1741-0126 (Print)
VL  - Vol. 18
IS  - Iss. 7
SP  - 345
EP  - 357
ER  - 
TY  - CHAP
A1  - Siegert, Petra
A1  - Iding, Hans
A1  - Baumann, Martin
A1  - McLeish, Michael J.
A1  - Kenyon, George L.
A1  - Pohl, Martina
T1  - Broadening of the substrate spectra of two ThDP-dependent decarboxylases using site-directed-mutagenesis
T2  - Proceedings of the 4th International Congress on Biochemical Engineering : 17 and 18 February 2000, Stuttgart
Y1  - 2000
SN  - 3-8167-5570-4
SP  - 38
EP  - 42
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Evers, Stefan
A1  - Marion, Merkel
A1  - Mussmann, Nina
A1  - Hellmuth, Hendrik
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
A1  - Schwaneberg, Ulrich
A1  - Martinez, Ronny
A1  - Jakob, Felix
T1  - Verfahren zur Anpassung eines hydrolytischen Enzyms an eine das hydrolytische Enzym stabilisierende Komponente [Offenlegungsschrift]
T1  - Method for adapting a hydrolytic enzyme to a component that stabilizes the hydrolytic enzyme [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2013
SP  - 1
EP  - 27
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - RPRT
A1  - Siegert, Petra
A1  - Bongaerts, Johannes
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
A1  - Selmer, Thorsten
T1  - Abschlussbericht zum Projekt zur Überwachung biotechnologischer Prozesse mittels Diacetyl-/Acetoin-Biosensor und Evaluierung von Acetoin-Reduktasen zur Verwendung in Biotransformationen
Y1  - 2022
N1  - Laufzeit: 01.01.2016 – 31.12.2019 (verlängert bis 31.12.2020)

Förderkennzeichen: 322-8.03.04.02-FH-Struktur 2016/02

Gefördert durch: Ministerium für Innovation, Wissenschaft und Forschung des Landes Nordrhein-Westfalen
CY  - Aachen
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Baumstark, Rebecca
A1  - Kluin, Cornelia
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
A1  - Hellmuth, Hendrik
T1  - Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]
Y1  - 2010
SP  - 1
EP  - 30
PB  - Deutsches Patent- und Markenamt
CY  - München
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Bankowski, Bernhard
T1  - Verwendung von Steroidsulfatase-Inhibitoren zur Verminderung von Haarausfall ; Kosmetische und pharmazeutische Zusammensetzungen enthaltend Steroidsulfatase-Inhibitoren und deren Verwendung zur Verminderung von Haarausfall [Offenlegungsschrift]
T1  - Cosmetic and pharmaceutical compositions comprising steroid sulfatase inhibitors and their use for the reduction of hair loss [Europäische Patentanmeldung]
Y1  - 2003
SP  - 1
EP  - 38
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt
CY  - München / Den Hague
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Yu, Lihua
A1  - Blaser, Martin
A1  - Andrei, Paula I.
T1  - 4-Hydroxyphenylacetate decarboxylases: properties of a novel subclass of glycyl radical enzyme systems / Yu, L. ; Blaser, M. ; Andrei, PI. ; Pierik, AJ. Selmer, T.
JF  - Biochemistry. 31 (2006), H. 45
Y1  - 2006
N1  - PMID: 16878993
SP  - 9584
EP  - 9592
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Willanzheimer, Angela
A1  - Hetzel, Marc
T1  - Propionate CoA-transferase from Clostridium propionicum. Cloning of the gene and identification of glutamate 324 at the active site
JF  - European Journal of Biochemistry. 269 (2002), H. 1
Y1  - 2002
SN  - 0014-2956
SP  - 372
EP  - 380
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Thamer, Wiebke
A1  - Cirpus, Irina
A1  - Hans, Marcus
T1  - A two [4Fe-4S]-cluster-containing ferredoxin as an alternative electron donor for 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans / Thamer, Wiebke ; Cirpus, Irina ; Hans, Marcus ; Pierik, Antonio, J. ; Selmer, Thorsten ; Bill, Eckhard ;
JF  - Archives of Microbiology. 179 (2003), H. 3
Y1  - 2003
SN  - 1432-072X
SP  - 197
EP  - 204
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Sommerlade, Hans-Jörg
A1  - Ingendoh, Arnd
A1  - Gieselmann, Volkmar
T1  - Glycosylation and phosphorylation of arylsulfatase A / Sommerlade, Hans-Jörg. ; Selmer, Thomas. ; Ingendoh, Arnd ; Gieselmann, Volkmar ; Figura, Kurt von ; Neifer, Klaus ; Schmidt, Bernhard
JF  - Journal of Biological Chemistry. 269 (1994), H. 33
Y1  - 1994
SN  - 1083-351X
SP  - 20977
EP  - 20981
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Scott, Richard
A1  - Näser, Ulrike
A1  - Friedrich, Peter
T1  - Stereochemistry of hydrogen removal from the 'unactivated' C-3 position of 4-hydroxybutyryl-CoA catalysed by 4-hydroxybutyryl-CoA dehydratase / Scott, R. ; Näser, U. ; Friedrich, P. ; Selmer, T. ; Buckel, W. ; Golding, BT.
JF  - Chemical Communications : ChemCom (2004)
Y1  - 2004
SN  - 1364-548X
SP  - 1210
EP  - 1211
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Schmidt, Bernhard
A1  - Ingendoh, Arnd
A1  - Figura, Kurt von
T1  - A novel amino acid modification in sulfatases that is defective in multiple sulfatase deficiency / Schmidt, Bernhard ; Selmer, Thorsten ;  Ingendoh, Arnd ;  Figurat, Kurt von
JF  - Cell. 82 (1995), H. 2
Y1  - 1995
SN  - 0092-8674
SP  - 271
EP  - 278
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Recksiek, Michael
A1  - Dierks, Thomas
A1  - Schmidt, Bernhard
T1  - Sulfatases, Trapping of the Sulfated Enzyme Intermediate by Substituting the Active Site Formylglycine / Recksiek, Michael ; Selmer, Thorsten ; Dierks, Thomas ; Schmidt, Bernhard ; Figura, Kurt von
JF  - Journal of Biological Chemistry. 273 (1998), H. 11
Y1  - 1998
SN  - 1083-351X
SP  - 6096
EP  - 6103
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Pinkenburg, Olaf
T1  - Method of cloning at least one nucleic acid molecule of interest using type IIS restriction endonucleases, and corresponding cloning vectors, kits and system using type IIS restriction endonucleases / Selmer, Thorsten ; Pinkenburg, Olaf
Y1  - 2008
N1  - Patent No. WO 2008095927  	A1  	Aug 14, 2008
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Pierik, Antonio J.
A1  - Heider, Johann
T1  - New glycyl radical enzymes catalysing key metabolic steps in anaerobic bacteria
JF  - Biological Chemistry. 386 (2005), H. 10
Y1  - 2005
SN  - 1431-6730
SP  - 981
EP  - 988
ER  -