TY  - JOUR
A1  - Prielmeier, Franz
A1  - Radkowitsch, H.
A1  - Lang, E. W.
A1  - Lüdemann, H.-D.
T1  - Density dependence of the molecular dynamics of fluid CH3F and CF3H studied by NMR / H. Radkowitsch, F. X.  Prielmeier, E. W. Lang and H.-D. Lüdemann
JF  - Physica B+C. 139-140 (1986)
Y1  - 1986
SN  - 0921-4526
SP  - 96
EP  - 99
ER  - 
TY  - JOUR
A1  - Kotter, Michael
A1  - Riekert, L.
A1  - Weyland, F.
T1  - Deposition of ternary oxides as active components by impregnation of porous carriers
JF  - Preparation of Catalysts III : scientific bases for the preparation of heterogeneous catalysts ; proceedings of the Third International Symposium, Louvain-la-Neuve, September 6-9, 1982 / ed.: G. Poncelet ... - (Studies in surface science and catalysis ; 16)
Y1  - 1983
SN  - 0-444-42184-X
N1  - International Symposium on the Scientific Bases for the Preparation of Heterogeneous Catalysts <3, 1982, Louvain-la-Neuve>
SP  - 521
EP  - 530
PB  - Elsevier
CY  - Amsterdam [u.a.]
ER  - 
TY  - JOUR
A1  - Hoffstadt, Kevin
A1  - Cheenakula, Dheeraja
A1  - Nikolausz, Marcell
A1  - Krafft, Simone
A1  - Harms, Hauke
A1  - Kuperjans, Isabel
T1  - Design and construction of a new reactor for flexible biomethanation of hydrogen
JF  - Fermentation
N2  - The increasing share of renewable electricity in the grid drives the need for sufficient storage capacity. Especially for seasonal storage, power-to-gas can be a promising approach. Biologically produced methane from hydrogen produced from surplus electricity can be used to substitute natural gas in the existing infrastructure. Current reactor types are not or are poorly optimized for flexible methanation. Therefore, this work proposes a new reactor type with a plug flow reactor (PFR) design. Simulations in COMSOL Multiphysics ® showed promising properties for operation in laminar flow. An experiment was conducted to support the simulation results and to determine the gas fraction of the novel reactor, which was measured to be 29%. Based on these simulations and experimental results, the reactor was constructed as a 14 m long, 50 mm diameter tube with a meandering orientation. Data processing was established, and a step experiment was performed. In addition, a kLa of 1 h−1 was determined. The results revealed that the experimental outcomes of the type of flow and gas fractions are in line with the theoretical simulation. The new design shows promising properties for flexible methanation and will be tested.
KW  - methanation
KW  - plug flow reactor
KW  - bubble column
KW  - bio-methane
KW  - power-to-gas
Y1  - 2023
U6  - http://dx.doi.org/10.3390/fermentation9080774
SN  - 2311-5637
N1  - The article belongs to the Special Issue Fermentation Processes: Modeling, Optimization and Control
VL  - 9
IS  - 8
SP  - 1
EP  - 16
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Kalbe, Jochen
A1  - Höcker, Hartwig
A1  - Berndt, Heinz
T1  - Design of enzyme reactors as chromatographic columns for racemic resolution of amino acid esters
JF  - Chromatographia
Y1  - 1989
SN  - 0009-5893
U6  - http://dx.doi.org/10.1007/BF02319646
VL  - 28
IS  - 3-4
SP  - 193
EP  - 196
ER  - 
TY  - JOUR
A1  - Leurs, Ulrike
A1  - Mezo, Gabor
A1  - Öhlschläger, Peter
A1  - Orban, Erika
A1  - Marquard, Andrea
A1  - Manea, Marilena
T1  - Design, synthesis, in vitro stability and cytostatic effect of multifunctional anticancer drug-bioconjugates containing GnRH-III as a targeting moiety
JF  - Peptide Science
N2  - Bioconjugates containing the GnRH-III hormone decapeptide as a targeting moiety are able to deliver chemotherapeutic agents specifically to cancer cells expressing GnRH receptors, thereby increasing their local efficacy while limiting the peripheral toxicity. However, the number of GnRH receptors on cancer cells is limited and they desensitize under continuous hormone treatment. A possible approach to increase the receptor mediated tumor targeting and consequently the cytostatic effect of the bioconjugates would be the attachment of more than one chemotherapeutic agent to one GnRH-III molecule. Here we report on the design, synthesis and biochemical characterization of multifunctional bioconjugates containing GnRH-III as a targeting moiety and daunorubicin as a chemotherapeutic agent. Two different drug design approaches were pursued. The first one was based on the bifunctional [4Lys]-GnRH-III (Glp-His-Trp-Lys-His-Asp-Trp-Lys-Pro-Gly-NH2) containing two lysine residues in positions 4 and 8, whose ϵ-amino groups were used for the coupling of daunorubicin. In the second drug design, the native GnRH-III (Glp-His-Trp-Ser-His-Asp-Trp-Lys-Pro-Gly-NH2) was used as a scaffold; an additional lysine residue was coupled to the ϵ-amino group of 8Lys in order to generate two free amino groups available for conjugation of daunorubicin. The in vitro stability/degradation of all synthesized compounds was investigated in human serum, as well as in the presence of rat liver lysosomal homogenate. Their cellular uptake was determined on human breast cancer cells and the cytostatic effect was evaluated on human breast, colon and prostate cancer cell lines. Compared with a monofunctional compound, both drug design approaches resulted in multifunctional bioconjugates with increased cytostatic effect.
Y1  - 2012
U6  - http://dx.doi.org/10.1002/bip.21640
SN  - 1097-0282
VL  - 98
IS  - 1
SP  - 1
EP  - 10
PB  - Wiley
CY  - New York, NY
ER  - 
TY  - JOUR
A1  - Schmich, Peter
A1  - Ederer, Hanns J.
A1  - Ebert, Klaus H.
T1  - Detection and identification of free radicals in hydrocarbon pyrolysis by an iodine trapping method
JF  - Industrial & Engineering Chemistry Research. 31 (1992), H. 1
Y1  - 1992
SN  - 1520-5045
SP  - 29
EP  - 37
ER  - 
TY  - JOUR
A1  - Welden, Melanie
A1  - Severins, Robin
A1  - Poghossian, Arshak
A1  - Wege, Christina
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Detection of acetoin and diacetyl by a tobacco mosaic virus-assisted field-effect biosensor
JF  - Chemosensors
N2  - Acetoin and diacetyl have a major impact on the flavor of alcoholic beverages such as wine or beer. Therefore, their measurement is important during the fermentation process. Until now, gas chromatographic techniques have typically been applied; however, these require expensive laboratory equipment and trained staff, and do not allow for online monitoring. In this work, a capacitive electrolyte–insulator–semiconductor sensor modified with tobacco mosaic virus (TMV) particles as enzyme nanocarriers for the detection of acetoin and diacetyl is presented. The enzyme acetoin reductase from Alkalihalobacillus clausii DSM 8716ᵀ is immobilized via biotin–streptavidin affinity, binding to the surface of the TMV particles. The TMV-assisted biosensor is electrochemically characterized by means of leakage–current, capacitance–voltage, and constant capacitance measurements. In this paper, the novel biosensor is studied regarding its sensitivity and long-term stability in buffer solution. Moreover, the TMV-assisted capacitive field-effect sensor is applied for the detection of diacetyl for the first time. The measurement of acetoin and diacetyl with the same sensor setup is demonstrated. Finally, the successive detection of acetoin and diacetyl in buffer and in diluted beer is studied by tuning the sensitivity of the biosensor using the pH value of the measurement solution.
Y1  - 2022
U6  - http://dx.doi.org/10.3390/chemosensors10060218
SN  - 2227-9040
N1  - This article belongs to the Special Issue "Nanostructured Devices for Biochemical Sensing"
VL  - 10
IS  - 6
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Molinnus, Denise
A1  - Bäcker, Matthias
A1  - Siegert, Petra
A1  - Willenberg, H.
A1  - Poghossian, Arshak
A1  - Keusgen, M.
A1  - Schöning, Michael Josef
T1  - Detection of Adrenaline Based on Substrate Recycling Amplification
JF  - Procedia Engineering
N2  - An amperometric enzyme biosensor has been applied for the detection of adrenaline. The adrenaline biosensor has been prepared by modification of an oxygen electrode with the enzyme laccase that operates at a broad pH range between pH 3.5 to pH 8. The enzyme molecules were immobilized via cross-linking with glutaraldehyde. The sensitivity of the developed adrenaline biosensor in different pH buffer solutions has been studied.
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.proeng.2015.08.708
SN  - 1877-7058
N1  - Eurosensors 2015
VL  - 120
SP  - 540
EP  - 543
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Deterding, A.
A1  - Ulber, Roland
T1  - Determination of acetic acid in fermentation broth by gas-diffusion technique
JF  - Engineering in Life Sciences
N2  - Due to the interfering effects of acetic acid in many fermentation processes, a gas-diffusion technique was developed for the online determination of acetic acid. The measurements were accomplished with a flow diffusion analysis (FDA) unit from the TRACE Analytics GmbH, Braunschweig, Germany. The diffusion analysis is based on the UV-absorbance of acetic acid at 205 nm. The measurement was achieved by the separation of an acceptor and a carrier stream (acidified fermentation broth) using a gas permeable polytetrafluoroethylene (PTFE) membrane, whereby broth constituents that would otherwise disturb the UV-measurement of acetic acid, are held back efficiently. Merely, the fermentation by-products, e.g. formic acid, is capable of diffusing through the membrane. While formic acid can disturb the measurement, carbon dioxide does not absorb at 205 nm. The method operates with time-dependent sample enrichment. During the analysis, a small volume of the acceptor stream is stopped for a defined time interval in the acceptor chamber. During this period, the gaseous acetic acid diffuses through the membrane and is enriched in the acceptor chamber. Subsequently after the enrichment, the acceptor stream flows through a UV-detector. The intensity of the signal is proportional to the acetic acid concentration. Online measurements in bioreactors via a sterile filtration probe have been accomplished. A linear calibration in the range of 0.5–5.0 g/L acetic acid with a relative standard deviation of <5 % was obtained. A sampling rate of 8 samples per hour was possible. The system was applied for the determination of acetic acid in E. coli fermentation broth. The instrument is easy to clean, very user-friendly and does not require any toxic or expensive reagents.
Y1  - 2008
U6  - http://dx.doi.org/10.1002/elsc.200820227
VL  - 8
IS  - 1, Special Issue: Technical Systems for the Use in Life Sciences
SP  - 62
EP  - 67
ER  - 
TY  - JOUR
A1  - Werner, Frederik
A1  - Krumbe, Christoph
A1  - Schumacher, Katharina
A1  - Groebel, Simone
A1  - Spelthahn, Heiko
A1  - Stellberg, Michael
A1  - Wagner, Torsten
A1  - Yoshinobu, Tatsuo
A1  - Selmer, Thorsten
A1  - Keusgen, Michael
A1  - Baumann, Marcus
A1  - Schöning, Michael Josef
T1  - Determination of the extracellular acidification of Escherichia coli by a light-addressable potentiometric sensor
JF  - Physica status solidi (a) : applications and material science. 208 (2011), H. 6
Y1  - 2011
SN  - 1862-6319
SP  - 1340
EP  - 1344
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Türler, A.
A1  - Gäggeler, H. W.
A1  - Jost, D. T.
T1  - Determination of the Partial Electron-Capture- and Spontaneous-Fission Half-Lives of 254No / A. Türler, H.W. Gäggeler, D.T. Jost, P. Armbruster, W. Brüchle, H. Folger, F.P. Heßberger,  S. Hofmann,
JF  - Zeitschrift für Physik A Hadrons and Nuclei. 331 (1988), H. 3
Y1  - 1988
SN  - 0939-7922
SP  - 363
EP  - 364
ER  - 
TY  - JOUR
A1  - Küppers, Tobias
A1  - Steffen, Victoria
A1  - Hellmuth, Hendrik
A1  - O'Connell, Timothy
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - Wiechert, Wolfgang
T1  - Developing a new production host from a blueprint: Bacillus pumilus as an industrial enzyme producer
JF  - Microbial cell factories
Y1  - 2014
U6  - http://dx.doi.org/10.1186/1475-2859-13-46
SN  - 1475-2859 (E-Journal)
VL  - 13
SP  - Article No. 46
PB  - BioMed Central
CY  - London
ER  - 
TY  - JOUR
A1  - Molinnus, Denise
A1  - Muschallik, Lukas
A1  - Gonzalez, Laura Osorio
A1  - Bongaerts, Johannes
A1  - Wagner, Torsten
A1  - Selmer, Thorsten
A1  - Siegert, Petra
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Development and characterization of a field-effect biosensor for the detection of acetoin
JF  - Biosensors and Bioelectronics
N2  - A capacitive electrolyte-insulator-semiconductor (EIS) field-effect biosensor for acetoin detection has been presented for the first time. The EIS sensor consists of a layer structure of Al/p-Si/SiO₂/Ta₂O₅/enzyme acetoin reductase. The enzyme, also referred to as butane-2,3-diol dehydrogenase from B. clausii DSM 8716T, has been recently characterized. The enzyme catalyzes the (R)-specific reduction of racemic acetoin to (R,R)- and meso-butane-2,3-diol, respectively. Two different enzyme immobilization strategies (cross-linking by using glutaraldehyde and adsorption) have been studied. Typical biosensor parameters such as optimal pH working range, sensitivity, hysteresis, linear concentration range and long-term stability have been examined by means of constant-capacitance (ConCap) mode measurements. Furthermore, preliminary experiments have been successfully carried out for the detection of acetoin in diluted white wine samples.
Y1  - 2018
U6  - http://dx.doi.org/10.1016/j.bios.2018.05.023
VL  - 115
SP  - 1
EP  - 6
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Turek, Monika
A1  - Ketterer, Lothar
A1  - Claßen, Melanie
A1  - Berndt, Heinz
A1  - Elbers, Gereon
A1  - Krüger, Peter
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Development and Electrochemical Investigations of an EIS-(Electrolyte-Insulator-Semiconductor) based Biosensor for Cyanide Detection
JF  - Sensors
Y1  - 2007
SN  - 1424-8220
VL  - 7
IS  - 8
SP  - 1415
EP  - 1426
ER  - 
TY  - JOUR
A1  - Seifarth, Volker
A1  - Goßmann, Matthias
A1  - Grosse, J. O.
A1  - Becker, C.
A1  - Heschel, I.
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Development of a Bioreactor to Culture Tissue Engineered Ureters Based on the Application of Tubular OPTIMAIX 3D Scaffolds
JF  - Urologia Internationalis
Y1  - 2015
U6  - http://dx.doi.org/10.1159/000368419
SN  - 0042-1138
VL  - 2015
IS  - 95
SP  - 106
EP  - 113
PB  - Karger
CY  - Basel
ER  - 
TY  - JOUR
A1  - Dünkelmann, Pascal
A1  - Kolter-Jung, Doris
A1  - Nitsche, Adam
A1  - Demir, Ayhan S.
A1  - Siegert, Petra
A1  - Lingen, Bettina
A1  - Baumann, Martin
A1  - Pohl, Martina
A1  - Müller, Michael
T1  - Development of a donor-acceptor concept for enzymatic cross-coupling reactions of adehydes : the first asymmetric cross-benzoin condensation
JF  - Journal of the American Chemical Society
Y1  - 2002
SN  - 1520-5126 (E-Journal); 0002-7863 (Print)
VL  - Vol. 124
SP  - 12084
EP  - 12085
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Meissner, Petra
A1  - Schröder, Bernd
A1  - Herfurth, Cornelia
T1  - Development of a fixed bed bioreactor for the expansion of human hematopoietic progenitor cells / Meissner, Petra ; Schröder, Bernd ; Herfurth, Cornelia ; Biselli, Manfred
JF  - Cytotechnology. 30 (1999), H. 1
Y1  - 1999
SN  - 0920-9069
SP  - 227
EP  - 234
ER  - 
TY  - JOUR
A1  - Pilas, Johanna
A1  - Iken, Heiko
A1  - Selmer, Thorsten
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Development of a multi‐parameter sensor chip for the simultaneous detection of organic compounds in biogas processes
JF  - Physica status solidi (a)
N2  - An enzyme-based multi-parameter biosensor is developed for monitoring the concentration of formate, d-lactate, and l-lactate in biological samples. The sensor is based on the specific dehydrogenation by an oxidized β-nicotinamide adenine dinucleotide (NAD+)-dependent dehydrogenase (formate dehydrogenase, d-lactic dehydrogenase, and l-lactic dehydrogenase, respectively) in combination with a diaphorase from Clostridium kluyveri (EC 1.8.1.4). The enzymes are immobilized on a platinum working electrode by cross-linking with glutaraldehyde (GA). The principle of the determination scheme in case of l-lactate is as follows: l-lactic dehydrogenase (l-LDH) converts l-lactate into pyruvate by reaction with NAD+. In the presence of hexacyanoferrate(III), the resulting reduced β-nicotinamide adenine dinucleotide (NADH) is then regenerated enzymatically by diaphorase. The electrochemical detection is based on the current generated by oxidation of hexacyanoferrate(II) at an applied potential of +0.3 V vs. an Ag/AgCl reference electrode. The biosensor will be electrochemically characterized in terms of linear working range and sensitivity. Additionally, the successful practical application of the sensor is demonstrated in an extract from maize silage.
Y1  - 2015
U6  - http://dx.doi.org/10.1002/pssa.201431894
SN  - 1862-6319
VL  - 212
IS  - 6
SP  - 1306
EP  - 1312
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Aboulnaga, E. A.
A1  - Zou, H.
A1  - Selmer, Thorsten
A1  - Xian, M.
T1  - Development of a plasmid-based, tunable, tolC-derived expression system for application in Cupriavidus necator H16
JF  - Journal of Biotechnology
N2  - Cupriavidus necator H16 gains increasing attention in microbial research and biotechnological application due to its diverse metabolic features. Here we present a tightly controlled gene expression system for C. necator including the pBBR1-vector that contains hybrid promoters originating from C. necator native tolC-promoter in combination with a synthetic tetO-operator. The expression of the reporter gene from these plasmids relies on the addition of the exogenous inducer doxycycline (dc). The novel expression system offers a combination of advantageous features as; (i) high and dose-dependent recombinant protein production, (ii) tight control with a high dynamic range (On/Off ratio), which makes it applicable for harmful pathways or for toxic protein production, (iii) comparable cheap inducer (doxycycline, dc), (iv) effective at low inducer concentration, that makes it useful for large scale application, (v) rapid, diffusion controlled induction, and (vi) the inducer does not interfere within the cell metabolism. As applications of the expression system in C. necator H16, the growth ability on glycerol was enhanced by constitutively expressing the E. coli glpk gene-encoding for glycerol kinase. Likewise, we used the system to overcome the expression toxicity of mevalonate pathway in C. necator H16. With this system, the mevalonate-genes were successfully introduced in the host and the recombinant strains could produce about 200 mg/l mevalonate.
Y1  - 2018
U6  - http://dx.doi.org/10.1016/j.jbiotec.2018.03.007
SN  - 0168-1656
VL  - 274
SP  - 15
EP  - 27
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Hambach, B.
A1  - Runstadler, P. W.
A1  - Wandrey, Christian
T1  - Development of a reactor-integrated aeration system for cultivation of animal cells in fluidized beds / Hambach, B. ; Biselli, M. ; Runstadler, P.W. ; Wandrey, C.
JF  - Animal cell technology : developments, processes, and products ; ESACT, European Society for Animal Cell Technology, the 11th meeting / Ed. R. E. Spier
Y1  - 1992
SN  - 0750604212
SP  - 381
EP  - 385
PB  - Butterworth-Heinemann
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Röhlen, Desiree
A1  - Pilas, Johanna
A1  - Schöning, Michael Josef
A1  - Selmer, Thorsten
T1  - Development of an amperometric biosensor platform for the combined determination of l-Malic, Fumaric, and l-Aspartic acid
JF  - Applied Biochemistry and Biotechnology
N2  - Three amperometric biosensors have been developed for the detection of L-malic acid, fumaric acid, and L -aspartic acid, all based on the combination of a malate-specific dehydrogenase (MDH, EC 1.1.1.37) and diaphorase (DIA, EC 1.8.1.4). The stepwise expansion of the malate platform with the enzymes fumarate hydratase (FH, EC 4.2.1.2) and aspartate ammonia-lyase (ASPA, EC 4.3.1.1) resulted in multi-enzyme reaction cascades and, thus, augmentation of the substrate spectrum of the sensors. Electrochemical measurements were carried out in presence of the cofactor β-nicotinamide adenine dinucleotide (NAD+) and the redox mediator hexacyanoferrate (III) (HCFIII). The amperometric detection is mediated by oxidation of hexacyanoferrate (II) (HCFII) at an applied potential of + 0.3 V vs. Ag/AgCl. For each biosensor, optimum working conditions were defined by adjustment of cofactor concentrations, buffer pH, and immobilization procedure. Under these improved conditions, amperometric responses were linear up to 3.0 mM for L-malate and fumarate, respectively, with a corresponding sensitivity of 0.7 μA mM−1 (L-malate biosensor) and 0.4 μA mM−1 (fumarate biosensor). The L-aspartate detection system displayed a linear range of 1.0–10.0 mM with a sensitivity of 0.09 μA mM−1. The sensor characteristics suggest that the developed platform provides a promising method for the detection and differentiation of the three substrates.
Y1  - 2017
U6  - http://dx.doi.org/10.1007/s12010-017-2578-1
SN  - 1559-0291
VL  - 183
SP  - 566
EP  - 581
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Van der Pol, Jens J.
A1  - Joksch, B.
A1  - Eberhardt, R.
T1  - Development of an enzymatic multichannel flow injection analysis system for monitoring mammalian cell fermentations / Van der Pol, J.J.; Joksch, B.; Eberhardt, R.; Biselli, M.; Wandrey, C., Tramper, J.
JF  - Biosensors : fundamentals, technologies and applications ; contributions to the BMFT status seminar with international participation May, 12 to 14, 1991, Internationales Bildungs-Centrum Bogensee/Brandenburg, Germany / org. by ZIM-Zentralinstitut für Molekularbiologie, Berlin-Buch and GBF, Braunschweig. Ed. by F. Scheller
Y1  - 1992
SN  - 3527284370
N1  - GBF Monographs ; 17
SP  - 349
EP  - 352
PB  - VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Jahnke, J.
T1  - Die marine Planktonalge Phaeocystis globosa / Jahnke, J. ; Baumann, M.
JF  - Mikrokosmos. 75 (1986), H. 12
Y1  - 1986
SN  - 0026-3680
SP  - 357
EP  - 359
ER  - 
TY  - JOUR
A1  - Kowollik, S.
A1  - Schnitzler, Thomas
A1  - Biselli, Manfred
A1  - Krueger, R.
A1  - Zang, Werner
A1  - Peuscher, A.
A1  - Schillberg, S.
A1  - Fischer, R.
T1  - Die Rolle des Respirationsquotienten in der Zellkulturfermentation
JF  - Chemie Ingenieur Technik
Y1  - 2010
SN  - Chemie Ingenieur Tec
N1  - Special Issue: ProcessNet-Jahrestagung 2010 und 28. Jahrestagung der Biotechnologen
VL  - 82
IS  - 9
SP  - 1505
EP  - 1506
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Noll, T.
T1  - Dielectric spectroscopy in the cultivation of suspended and immobilized hybridoma cells / Noll, T.; Biselli, M.
JF  - Journal of Biotechnology. 63 (1998), H. 3
Y1  - 1998
SN  - 0168-1656
SP  - 187
EP  - 198
ER  - 
TY  - JOUR
A1  - Capitain, Charlotte
A1  - Ross-Jones, Jesse
A1  - Möhring, Sophie
A1  - Tippkötter, Nils
T1  - Differential scanning calorimetry for quantification of polymer biodegradability in compost
JF  - International Biodeterioration & Biodegradation
N2  - The objective of this study is the establishment of a differential scanning calorimetry (DSC) based method for online analysis of the biodegradation of polymers in complex environments. Structural changes during biodegradation, such as an increase in brittleness or crystallinity, can be detected by carefully observing characteristic changes in DSC profiles. Until now, DSC profiles have not been used to draw quantitative conclusions about biodegradation. A new method is presented for quantifying the biodegradation using DSC data, whereby the results were validated using two reference methods.

The proposed method is applied to evaluate the biodegradation of three polymeric biomaterials: polyhydroxybutyrate (PHB), cellulose acetate (CA) and Organosolv lignin. The method is suitable for the precise quantification of the biodegradability of PHB. For CA and lignin, conclusions regarding their biodegradation can be drawn with lower resolutions. The proposed method is also able to quantify the biodegradation of blends or composite materials, which differentiates it from commonly used degradation detection methods.
Y1  - 2020
U6  - http://dx.doi.org/10.1016/j.ibiod.2020.104914
SN  - 0964-8305
VL  - 149
SP  - In Press, Article number 104914
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Jahnke, J.
A1  - Baumann, Marcus
T1  - Differentiation between Phaeocystis pouchetii (Har.) Lagerheim and Phaeocystis globosa Scherffel
JF  - Hydrobiological bulletin
Y1  - 1987
SN  - 0165-1404 (Print); 1573-5125 (E-Journal)
VL  - Vol. 21
IS  - Iss. 2
SP  - 141
EP  - 147
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Schröder, B.
A1  - Herfurth, C.
A1  - Meißner, P.
T1  - Differentiation of bone marrow cells immobilized on microcarriers in a fluidized bed reactor / Schröder, B. ; Herfurth, C. ; Meißner, P. ; Biselli, M. ; Link, H. ; Wandrey, C.
JF  - Animal cell technology : from vaccines to genetic medicine ; [Vilamoura, Portugal, May 1996] / ed. by Manuel J. T. Carrondo
Y1  - 1997
SN  - 0-7923-4321-2
SP  - 589
EP  - 593
PB  - Kluwer
CY  - Dordrecht
ER  - 
TY  - JOUR
A1  - Kotter, Michael
A1  - Heering, J.
A1  - Riekert, L.
T1  - Diffusion and catalytic reaction in zeolite ZSM-5 / J. Heering ;  M. Kotter ; L. Riekert
JF  - Chemical engineering science  . 37 (1982), H. 4
Y1  - 1982
SN  - 0009-2509
SP  - 581
EP  - 584
ER  - 
TY  - JOUR
A1  - Prielmeier, Franz
A1  - Speedy, R. J.
A1  - Vardag, T.
A1  - Lang, E. W.
T1  - Diffusion in simple fluids / R. J. Speedy;  F. X. Prielmeier;  T. Vardag;  E. W. Lang; H.-D. Lüdemann
JF  - Molecular Physics. 66 (1989), H. 3
Y1  - 1989
SN  - 0026-8976
SP  - 577
EP  - 590
ER  - 
TY  - JOUR
A1  - Prielmeier, Franz
A1  - Lang, E. W.
A1  - Speedy, R. J.
A1  - Lüdemann, H.-D.
T1  - Diffusion in supercooled water to 300 MPa
JF  - Physical Review Letters. 59 (1987), H. 10
Y1  - 1987
SN  - 0031-9007
SP  - 1128
EP  - 1131
ER  - 
TY  - JOUR
A1  - Jeromin, Günter Erich
A1  - Welsch, Volker
T1  - Diketene a New Esterification Reagent in the Enzyme-Aided Synthesis of Chiral Alcohols and Chiral Acetoacetic Acid Esters
JF  - Tetrahedron Letters  . 36 (1995), H. 37
Y1  - 1995
SN  - 0040-4039
N1  - ISSN der E-Ausg.: 1359-8562
SP  - 6663
EP  - 6664
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Lara, R.J.
A1  - Hubberten, D.N.
A1  - Thomas, D.N.
T1  - Dissolved organic matter studies in enclosed systems: Application of hydophobic fractionation for the assessment of organic nitrogen dynamics / Lara, R.J. ; Hubberten, U. ; Thomas, D.N. ; Baumann, M.E.M. ; Kattner, G.
JF  - Journal of Marine Systems. 13 (1997), H. 1-4
Y1  - 1997
SN  - 0924-7963
SP  - 155
EP  - 161
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Tillmann, U.
A1  - Aletsee, L.
T1  - Distribution of Carbon Among Photosynthetic End Products in the Bloom-Forming Arctic Diatom Thalassiosira antarctica COMBER / Tillmann, U. ; Baumann, M.E.M. ; Aletsee, L.
JF  - Polar Biology. 10 (1989), H. 3
Y1  - 1989
SN  - 0722-4060
SP  - 231
EP  - 238
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Gradinger, R.
T1  - Distribution of Phytoplankton Communities in Relation to the Large Scale Hydrographical Regime in the Fram Strait 1984 / Gradinger, R. ; Baumann, M.E.M.
JF  - Marine Biology. 111 (1991), H. 2
Y1  - 1991
SN  - 0025-3162
SP  - 311
EP  - 321
ER  - 
TY  - JOUR
A1  - Bongaerts, Johannes
A1  - Esser, Simon
A1  - Lorbach, Volker
A1  - Al-Momani, Lóay
A1  - Müller, Michael A.
A1  - Franke, Dirk
A1  - Grondal, Christoph
A1  - Kurutsch, Anja
A1  - Bujnicki, Robert
A1  - Takors, Ralf
A1  - Raeven, Leon
A1  - Wubbolts, Marcel
A1  - Bovenberg, Roel
A1  - Nieger, Martin
A1  - Schürmann, Melanie
A1  - Trachtmann, Natalie
A1  - Kozak, Stefan
A1  - Sprenger, Georg A.
A1  - Müller, Michael
T1  - Diversity-oriented production of metabolites derived from chorismate and their use in organic synthesis
JF  - Angewandte Chemie International Edition
Y1  - 2011
SN  - 1521-3773 (E-Journal); 0570-0833 (Print); 1433-7851 (Print)
VL  - Vol. 50
IS  - Iss. 34
SP  - 7781
EP  - 7786
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Seibler, Jost
A1  - Schübeler, Dirk
A1  - Fiering, Steven
A1  - Groudine, Mark
A1  - Bode, Jürgen
T1  - DNA cassette exchange in ES cells mediated by Flp recombinase: an efficient strategy for repeated modification of tagged loci by marker-free constructs
JF  - Biochemistry
Y1  - 1998
U6  - http://dx.doi.org/10.1021/bi980288t
SN  - 1520-4995
VL  - 37
IS  - 18
SP  - 6229
EP  - 6234
ER  - 
TY  - JOUR
A1  - Seibler, Jost
A1  - Bode, Jürgen
T1  - Double-reciprocal crossover mediated by FLP-recombinase: a concept and an assay
JF  - Biochemistry
Y1  - 1997
SN  - 1520-4995
VL  - 36
IS  - 7
SP  - 1740
EP  - 1747
ER  - 
TY  - JOUR
A1  - Graf, Alain-Michel
A1  - Steinhof, Rafael
A1  - Lotz, Martin
A1  - Tippkötter, Nils
A1  - Kasper, Cornelia
A1  - Beutel, Sascha
A1  - Ulber, Roland
T1  - Downstream-Processing mit Membranadsorbern zur Isolierung nativer Proteinfraktionen aus Kartoffelfruchtwasser
JF  - Chemie Ingenieur Technik
N2  - Bei der Stärkeproduktion entstehendes Kartoffelfruchtwasser besitzt mit 2 – 3 % einen hohen Anteil an ernährungsphysiologisch interessanten Proteinen. Die industrielle Gewinnung dieser Proteinfracht liefert jedoch lediglich ein minderwertiges, denaturiertes Produkt. Mit Hilfe der Membranadsorber-Technologie lassen sich aus Kartoffelfruchtwasser unter milden Reaktionsbedingungen native bioaktive Proteinfraktionen gewinnen. Geeignete Trennbedingungen wurden im Labormaßstab entwickelt und in den Technikumsmaßstab übertragen. An Anionenaustauscher-Membranadsorbern mit einer Membranfläche von 10 000 cm2 wurde eine Patatinhaltige Fraktion (44 kDa) mit Bindungskapazitäten von 0,37 mg/cm2 isoliert. Eine niedermolekulare Proteinfraktion mit Protease-Inhibitoren konnte durch Kationenaustauscher-Membranadsorber mit Bindungskapazitäten von 1,00 mg/cm2 gewonnen werden. Sie ist für verschiedenste Applikationen in der pharmazeutischen, kosmetischen und der Nahrungsmittelindustrie interessant z. B. für Appetitzügler oder muskelaufbauende Proteinpräparate. Der Aufreinigung der nativen Proteinfraktionen durch Ultra-/Diafiltration schließt sich die Konfektionierung durch Sprühtrocknung an. Die bioanalytische Charakterisierung der Produkte belegt die Reinheit und die enzymatische Aktivität sowie die Abreicherung von Störkomponenten wie Glykoalkaloide und Polyphenoloxidasen.
Y1  - 2009
U6  - http://dx.doi.org/10.1002/cite.200800139
VL  - 81
IS  - 3
SP  - 267
EP  - 274
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Stanley, Lesley A.
A1  - Horsburgh, Brian C.
A1  - Ross, Jillian
A1  - Scheer, Nico
A1  - Wolf, C. Roland
T1  - Drug transporters: Gatekeepers controlling access of xenobiotics to the cellular interior
JF  - Drug Metabolism Reviews
Y1  - 2009
U6  - http://dx.doi.org/10.1080/03602530802605040
SN  - 1097-9883
VL  - 41
IS  - 1
SP  - 27
EP  - 65
PB  - Taylor & Francis
CY  - London
ER  - 
TY  - JOUR
A1  - Prielmeier, Franz
A1  - Merboldt, K. D.
A1  - Hanicke, W.
A1  - Frahm, J.
T1  - Dynamic high-resolution MR imaging of brain deoxygenation during transient anoxia in the anesthetized rat
JF  - Journal of cerebral blood flow and metabolism. 13 (1993), H. 5
Y1  - 1993
SN  - 0271-678X
SP  - 889
EP  - 894
ER  - 
TY  - JOUR
A1  - Prielmeier, Franz
A1  - Nagatomo, Yasushi
A1  - Wick, Markus
A1  - Frahm, Jens
T1  - Dynamic monitoring of cerebral metabolites during and after transient global ischemia in rats by quantitative proton NMR spectroscopy in vivo / Yasushi Nagatomo, Markus Wick, Franz Prielmeier, Jens Frahm
JF  - NMR in Biomedicine. 8 (1995), H. 6
Y1  - 1995
SN  - 1099-1492
SP  - 265
EP  - 270
ER  - 
TY  - JOUR
A1  - Abulnaga, El-Hussiny
A1  - Pinkenburg, Olaf
A1  - Schiffels, Johannes
A1  - E-Refai, Ahmed
A1  - Buckel, Wolfgang
A1  - Selmer, Thorsten
T1  - Effect of an Oxygen-Tolerant Bifurcating Butyryl Coenzyme A Dehydrogenase/Electron-Transferring Flavoprotein Complex from Clostridium difficile on Butyrate Production in Escherichia coli
JF  - Journal of bacteriology
Y1  - 2013
SN  - 1098-5530 [E-Journal]
SN  - 0021-9193 [Print]
VL  - 195
IS  - 16
SP  - 3704
EP  - 3713
ER  - 
TY  - JOUR
A1  - Heinze, D.
A1  - Mang, Thomas
A1  - Popescu, C.
A1  - Weichold, O.
T1  - Effect of side chain length and degree of polymerization on the decomposition and crystallization behaviour of chlorinated poly(vinyl ester) oligomers
JF  - Thermochimica Acta
N2  - Four members of a homologous series of chlorinated poly(vinyl ester) oligomers CCl₃–(CH₂CH (OCO(CH₂)ₘCH₃))ₙ–Cl with degrees of polymerization of 10 and 20 were prepared by telomerisation using carbon tetrachloride. The number of side chain carbon atoms ranges from 2 (poly(vinyl acetate) to 18 (poly(vinyl stearate)). The effect of the n-alkyl side chain length and of the degree of polymerization on the thermal stability and crystallization behaviour of the synthesized compounds was investigated.

All oligomers degrade in two major steps by first losing HCl and side chains with subsequent breakdown of the backbone. The members with short side chains, up to poly(vinyl octanoate), are amorphous and show internal plasticization, whereas those with high number of side chain carbon atoms are semi-crystalline due to side-chain crystallization. A better packing for poly(vinyl stearate) is also noticeable. The glass transition and melting temperatures as well as the onset temperature of decomposition are influenced to a larger extent by the side chain length than by the degree of polymerization. Thermal stability is improved if both the size and number of side chains increase, but only a long side chain causes a significant increase of the resistance to degradation. This results in a stabilization of PVAc so that oligomers from poly(vinyl octanoate) on are stable under atmospheric conditions. Thus, the way to design stable, chlorinated PVEs oligomers is to use a long n-alkyl side chain.
Y1  - 2016
U6  - http://dx.doi.org/10.1016/j.tca.2016.05.015
SN  - 0040-6031 (electronic)
VL  - 637
SP  - 143
EP  - 153
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Cehreli, Ruksan
A1  - Akpinar, Hale
A1  - Temiz Artmann, Aysegül
A1  - Sagol, Ozgul
T1  - Effects of Glutamine and Omega-3 Fatty Acids on Erythrocyte Deformability and Oxidative Damage in Rat Model of Enterocolitis
JF  - Gastroenterology Research
Y1  - 2015
U6  - http://dx.doi.org/10.14740/gr683w
SN  - 1918-2813
VL  - 8
IS  - 5
SP  - 265
EP  - 273
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Thomas, D.
A1  - Gleitz, M.
T1  - Efficiency of carbon assimilation and photoacclimation in a small unicellular Chaetoceros species from the Weddel Sea (Antarctica): Influence of temperature and irridiance / Thomas, D. ; Baumann, M.E.M. ; Gleitz, M.
JF  - Journal of Experimental Marine Biology and Ecology. 157 (1992), H. 2
Y1  - 1992
SN  - 0022-0981
SP  - 195
EP  - 209
ER  - 
TY  - JOUR
A1  - Iber, Michaela
A1  - Schübeler, Dirk
A1  - Seibler, Jost
A1  - Höxter, Maria
A1  - Bode, Jürgen
T1  - Efficient FACS selection procedure for cells undergoing Flp-mediated site-specific conversions
JF  - Technical Tips Online
Y1  - 1998
U6  - http://dx.doi.org/10.1016/S1366-2120(08)70132-6
VL  - 4
IS  - 1
SP  - 25
EP  - 29
ER  - 
TY  - JOUR
A1  - Meyer-Stork, L. Sebastian
A1  - Kalbe, Jochen
A1  - Kuropka, Rolf
A1  - Sauter, S. L.
A1  - Höcker, Hartwig
A1  - Berndt, Heinz
T1  - Eindeutige Provenienzanalyse von Wolle und feinen Tierhaaren mit Hilfe der Erbsubstanz (DNS)
JF  - Textilveredlung : schweizerische Zeitschrift für Textilchemie, Textilveredlung und deren Randgebiete
Y1  - 1988
SN  - 0040-5310
VL  - 23
IS  - 9
SP  - 304
EP  - 307
ER  - 
TY  - JOUR
A1  - Kotter, Michael
A1  - Kirch, M.
A1  - Riekert, Lothar
T1  - Einfluß von Dispersion und Vorgeschichte dispersen Nickels auf dessen katalytische Eigenschaften bei der Hydrierung von Benzol, CO2 und CO / R. Kirch ; M. Kotter ; L. Riekert
JF  - Berichte der Bunsen-Gesellschaft für Physikalische Chemie. 88 (1984)
Y1  - 1984
SN  - 0005-9021
SP  - 1054
EP  - 1057
ER  - 
TY  - JOUR
A1  - Mang, Thomas
T1  - Elastomere zum Abdichten gegen drückendes Wasser
JF  - Summaries : June 30 to July 3, 2003, Nürnberg = Kurzfassungen
Y1  - 2003
N1  - Internationale Kautschuk-Tagung ; <2003 : ; 2003.06.30-07.03 : ; Nürnberg> ;  International Rubber Conference<2003, Nürnberg>
SP  - 121
EP  - 123
PB  - DKG, Dt. Kautschuk-Ges.
CY  - Frankfurt, Main
ER  - 
TY  - JOUR
A1  - Mang, Thomas
T1  - Elastomere zum Abdichten gegen drückendes Wasser
JF  - Kautschuk Gummi Kunststoffe. 57 (2004), H. 4
Y1  - 2004
SN  - 0022-9520
SP  - 172
EP  - 178
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Srivastava, Alok
A1  - Singh, Vivendra
A1  - Chandra, Amita
T1  - Electrical conductivity studies of swift heavy ion modified PVC and PVC-PANI composite / Alok Srivastava ,Virendra Singh, Amita Chandra, K.Witte, U.W.Scherer and T.V.Singh
JF  - Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms. 245 (2006), H. 1
Y1  - 2006
SN  - 0168-583X
SP  - 277
EP  - 280
ER  - 
TY  - JOUR
A1  - Bäcker, Matthias
A1  - Delle, L.
A1  - Poghossian, Arshak
A1  - Biselli, Manfred
A1  - Zang, Werner
A1  - Wagner, P.
A1  - Schöning, Michael Josef
T1  - Electrochemical sensor array for bioprocess monitoring
JF  - Electrochimica Acta (2011)
Y1  - 2011
VL  - 56
IS  - 26
SP  - 9673
EP  - 9678
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Lehmann, G.
T1  - Electron Paramagnetic Resonance and Optical Absorption Spectra of VO2+ in CsCl Single Crystals
JF  - Zeitschrift für Naturforschung / Section A, a journal of physical sciences. 40 (1985)
Y1  - 1985
SN  - 0932-0784
SP  - 511
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Lehmann, G.
T1  - Electron paramagnetic resonance of Vanadyl ion impurities in crystalline solids: A comment
JF  - Journal of Physics and Chemistry of Solids. 46 (1985), H. 6
Y1  - 1985
SN  - 0022-3697
SP  - 761
ER  - 
TY  - JOUR
A1  - Cesari, Francesca
A1  - Rennekampff, Verena
A1  - Vintersten, Kristina
A1  - Vuong, Lam Giang
A1  - Seibler, Jost
A1  - Bode, Jürgen
A1  - Wiebel, Franziska F.
A1  - Nordheim, Alfred
T1  - Elk-1 knock-out mice engineered by Flp recombinase-mediated cassette exchange
JF  - Genesis : The Journal of Genetics and Development
Y1  - 2004
U6  - http://dx.doi.org/10.1002/gene.20003
SN  - 1526-968X
VL  - 38
IS  - 2
SP  - 87
EP  - 92
ER  - 
TY  - JOUR
A1  - Müller, Janina
A1  - Beckers, Mario
A1  - Mußmann, Nina
A1  - Bongaerts, Johannes
A1  - Büchs, Jochen
T1  - Elucidation of auxotrophic deficiencies of Bacillus pumilus DSM 18097 to develop a defined minimal medium
JF  - Microbial Cell Factories
N2  - Background
Culture media containing complex compounds like yeast extract or peptone show numerous disadvantages. The chemical composition of the complex compounds is prone to significant variations from batch to batch and quality control is difficult. Therefore, the use of chemically defined media receives more and more attention in commercial fermentations. This concept results in better reproducibility, it simplifies downstream processing of secreted products and enable rapid scale-up. Culturing bacteria with unknown auxotrophies in chemically defined media is challenging and often not possible without an extensive trial-and-error approach. In this study, a respiration activity monitoring system for shake flasks and its recent version for microtiter plates were used to clarify unknown auxotrophic deficiencies in the model organism Bacillus pumilus DSM 18097.

Results
Bacillus pumilus DSM 18097 was unable to grow in a mineral medium without the addition of complex compounds. Therefore, a rich chemically defined minimal medium was tested containing basically all vitamins, amino acids and nucleobases, which are essential ingredients of complex components. The strain was successfully cultivated in this medium. By monitoring of the respiration activity, nutrients were supplemented to and omitted from the rich chemically defined medium in a rational way, thus enabling a systematic and fast determination of the auxotrophic deficiencies. Experiments have shown that the investigated strain requires amino acids, especially cysteine or histidine and the vitamin biotin for growth.

Conclusions
The introduced method allows an efficient and rapid identification of unknown auxotrophic deficiencies and can be used to develop a simple chemically defined tailor-made medium. B. pumilus DSM 18097 was chosen as a model organism to demonstrate the method. However, the method is generally suitable for a wide range of microorganisms. By combining a systematic combinatorial approach based on monitoring the respiration activity with cultivation in microtiter plates, high throughput experiments with high information content can be conducted. This approach facilitates media development, strain characterization and cultivation of fastidious microorganisms in chemically defined minimal media while simultaneously reducing the experimental effort.
Y1  - 2018
U6  - http://dx.doi.org/10.1186/s12934-018-0956-1
SN  - 1475-2859
VL  - 17
IS  - 1
SP  - Article No. 106
PB  - BioMed Central
ER  - 
TY  - JOUR
A1  - Krüger, Götz
A1  - Grötzinger, Joachim
A1  - Berndt, Heinz
T1  - Enantiomeric resolution of amino acid derivatives on chiral stationary phases by high-performance liquid chromatography
JF  - Journal of Chromatography A
Y1  - 1987
U6  - http://dx.doi.org/10.1016/S0021-9673(01)85005-6
SN  - 0021-9673
VL  - 1987
IS  - 397
SP  - 223
EP  - 232
ER  - 
TY  - JOUR
A1  - Dünnwald, Thomas
A1  - Demir, Ayhan S.
A1  - Siegert, Petra
A1  - Pohl, Martina
A1  - Müller, Michael
T1  - Enantioselective Synthesis of (S)-2-Hydroxypropanone Derivatives by Benzoylformate Decarboxylase Catalyzed C−C Bond Formation
JF  - European journal of organic chemistry
Y1  - 2000
SN  - 0365-5490 (E-Journal); 1099-0690 (E-Journal); 0075-4617 (Print); 0170-2041 (Print); 0947-3440 (Print); 1434-193X (Print); 1434-243X (Print)
VL  - Vol. 2000
IS  - Iss. 11
SP  - 2161
EP  - 2170
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
T1  - Energetische Verwertung von Kunststoffabfällen
JF  - Chemie und Technologie makromolekularer Stoffe :, 16. Kolloquium.  / Fachhochschule <Aachen> / Fachbereich Chemieingenieurwesen. 20. November 1998 an der FH Aachen, Fachbereich Chemieingenieurwesen
Y1  - 1998
N1  - FH-Texte. 69; In Jülich unter der Signatur: 61 ZOW 405-16
SP  - 169
EP  - 189
PB  - Fachhochschule Aachen
CY  - Aachen
ER  - 
TY  - JOUR
A1  - Manea, Marilena
A1  - Leurs, Ulrike
A1  - Orban, Erika
A1  - Baranyai, Zsuzsa
A1  - Öhlschläger, Peter
A1  - Marquardt, Andreas
A1  - Schulcz, Akos
A1  - Tejeda, Miguel
T1  - Enhanced Enzymatic Stability and Antitumor Activity of Daunorubicin-GnRH-III Bioconjugates Modified in Position 4
JF  - Bioconjugate Chemistry
Y1  - 2011
SN  - 1520-4812
VL  - 22
IS  - 7
SP  - 1320
EP  - 1329
PB  - ACS
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Gerigk, M.
A1  - Maaß, D.
A1  - Kreutzer, A.
A1  - Sprenger, G.
A1  - Bongaerts, Johannes
A1  - Wubbolts, Marcel
A1  - Takors, Ralf
T1  - Enhanced pilot-scale fed-batch L-phenylalanine production with recombinant Escherichia coli by fully integrated reactive extraction
JF  - Bioprocess and biosystems engineering
Y1  - 2002
SN  - 1432-0797 (E-Journal); 1615-7605 (E-Journal); 0178-515X (Print); 1615-7591 (Print)
VL  - Vol. 25
IS  - Iss. 1
SP  - 43
EP  - 52
ER  - 
TY  - JOUR
A1  - Plum, Leona
A1  - Ma, Xiaosong
A1  - Hampel, Brigitte
A1  - Balthasar, Nina
A1  - Coppari, Roberto
A1  - Münzberg, Heike
A1  - Shanabrough, Marya
A1  - Burdakov, Denis
A1  - Rother, Eva
A1  - Janoschek, Ruth
A1  - Alber, Jens
A1  - Belgardt, Bengt F.
A1  - Koch, Linda
A1  - Seibler, Jost
A1  - Schenk, Frieder
A1  - Fekete, Csaba
A1  - Suzuki, Akira
A1  - Mak, Tak W.
A1  - Krone, Wilhelm
A1  - Horvath, Tamas L.
A1  - Ashcroft, Frances M.
A1  - Brüning, Jens C.
T1  - Enhanced PIP3 signaling in POMC neurons causes KATP channel activation and leads to diet-sensitive obesity
JF  - The Journal of Clinical Investigation (JCI)
Y1  - 2006
U6  - http://dx.doi.org/10.1172/JCI27123
SN  - 1558-8238
VL  - 116
IS  - 7
SP  - 1886
EP  - 1901
ER  - 
TY  - JOUR
A1  - Öhlschläger, Peter
A1  - Quetting, Michael
A1  - Alvarez, Gerardo
A1  - Dürst, Matthias
A1  - Gissmann, Lutz
A1  - Kaufmann, Andreas M.
T1  - Enhancement of immunogenicity of a therapeutic cervical cancer DNA-based vaccine by co-application of sequence-optimized genetic adjuvants
JF  - International Journal of Cancer
Y1  - 2009
SN  - 1097-0215
VL  - 125
IS  - 1
SP  - 189
EP  - 198
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Kotter, Michael
A1  - Riekert, Lothar
A1  - Turek, Thomas
T1  - Entfernung von Ammoniak aus Abwässern durch Strippung und Sorption an Zeolithen : [Synopse 1724]
JF  - Chemie - Ingenieur - Technik. 61 (1989), H. 1
Y1  - 1989
SN  - 0009-286X
N1  - ISSN der E-Ausg.: 1522-2640; Das vollständige Manuskript dieser Arbeit umfasst 45 Seiten. Es ist [lt. Verl.] als Fotokopie oder Mikrofiche MS 1724/88 erhältlich.
SP  - 74
EP  - 75
ER  - 
TY  - JOUR
A1  - Mang, Thomas
A1  - Haulena, Friedhelm
A1  - Brill, B.
T1  - Entsorgung beflockter Verbundwerkstoffe
JF  - Flock 97 : 14. Internationales Flockseminar Seeheim bei Darmstadt 16. - 17. Januar 1997.
Y1  - 1997
N1  - International Flock Symposium <14, 1997, Seeheim>
SP  - 10-1
EP  - 10-18
PB  - Flock-Verl.
CY  - Büdingen
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Bäcker, Matthias
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
A1  - Schnitzler, Thomas
A1  - Zang, Werner
A1  - Wagner, P.
T1  - Entwicklung eines modularen festkörperbasierten Sensorsystems für die Überwachung von Zellkulturfermenationen
JF  - Sensoren und Messsysteme 2010 [Elektronische Ressource] : Vorträge der 15. ITG/GMA-Fachtagung vom 18. bis 19. Mai 2010 in Nürnberg / Informationstechnische Gesellschaft im VDE (ITG); VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik (GMA)
Y1  - 2010
SN  - 978-3-8007-3260-9
N1  - Fachtagung Sensoren und Messsysteme 15, 2010, Nürnberg ; Gesellschaft Mess- und Automatisierungstechnik
SP  - 688
EP  - 691
PB  - VDE Verlag
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Deppe, Veronika Maria
A1  - Bongaerts, Johannes
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
A1  - Meinhardt, Friedhelm
T1  - Enzymatic deglycation of Amadori products in bacteria
JF  - Applied microbiology and biotechnology
Y1  - 2011
SN  - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)
VL  - Vol. 90
IS  - Iss. 2
SP  - 399
EP  - 406
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Duwe, Anna-Maria
A1  - Wiesen, Sebastian
A1  - Sieker, Tim
A1  - Ulber, Roland
T1  - Enzymatic hydrolysis of beech wood lignocellulose at high solid contents and its utilization as substrate for the production of biobutanol and dicarboxylic acids
JF  - Bioresource Technology
N2  - The development of a cost-effective hydrolysis for crude cellulose is an essential part of biorefinery developments. To establish such high solid hydrolysis, a new solid state reactor with static mixing is used. However, concentrations >10% (w/w) cause a rate and yield reduction of enzymatic hydrolysis. By optimizing the synergetic activity of cellulolytic enzymes at solid concentrations of 9%, 17% and 23% (w/w) of crude Organosolv cellulose, glucose concentrations of 57, 113 and 152 g L⁻¹ are reached. However, the glucose yield decreases from 0.81 to 0.72gg⁻¹ at 17% (w/w). Optimal conditions for hydrolysis scale-up under minimal enzyme addition are identified. As result, at 23% (w/w) crude cellulose the glucose yield increases from 0.29 to 0.49gg⁻¹. As proof of its applicability, biobutanol, succinic and itaconic acid are produced with the crude hydrolysate. The potential of the substrate is proven e.g. by a high butanol yield of 0.33gg⁻¹.
Y1  - 2014
U6  - http://dx.doi.org/10.1016/j.biortech.2014.06.052
VL  - 167
SP  - 447
EP  - 455
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Jeromin, Günter Erich
A1  - Weise, Sven
T1  - Enzymatische Acetylierung von (±)-1-Phthalimido-2-propanol
JF  - Liebigs Annalen der Chemie (1990)
Y1  - 1990
SN  - 0947-3440
N1  - ISSN der E-Ausg.: 1099-0690
SP  - 1045
EP  - 1046
ER  - 
TY  - JOUR
A1  - Niehaus, F.
A1  - Gabor, E.
A1  - Wieland, S.
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
A1  - Eck, J.
T1  - Enzymes for the laundry industries: tapping the vast metagenomic pool of alkaline proteases
JF  - Microbial biotechnology
Y1  - 2011
SN  - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)
VL  - Vol. 4
IS  - Iss. 6
SP  - 767
EP  - 776
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Remme, S.
A1  - Lehmann, G.
T1  - EPR and Optical Absorption of Cr3+ in CsCl and CsBr
JF  - Physica Status Solidi (B). 142 (1987), H. 2
Y1  - 1987
SN  - 0031-8957
SP  - 367
EP  - 377
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Remme, S.
A1  - Lehmann, G.
T1  - EPR of Cr3+ in Tris(acetylacetonato)gallium(III) Single Crystals
JF  - Inorganic Chemistry. 25 (1986)
Y1  - 1986
SN  - 0020-1669
SP  - 896
EP  - 897
ER  - 
TY  - JOUR
A1  - Sieker, Tim
A1  - Neuner, Andreas
A1  - Dimitrova, Darina
A1  - Tippkötter, Nils
A1  - Muffler, Kai
A1  - Bart, Hans-Jörg
A1  - Heinzle, Elmar
A1  - Ulber, Roland
T1  - Ethanol production from grass silage by simultaneous pretreatment, saccharification and fermentation: First steps in the process development
JF  - Engineering in Life Sciences
N2  - Grass silage provides a great potential as renewable feedstock. Two fractions of the grass silage, a press juice and the fiber fraction, were evaluated for their possible use for bioethanol production. Direct production of ethanol from press juice is not possible due to high concentrations of organic acids. For the fiber fraction, alkaline peroxide or enzymatic pretreatment was used, which removes the phenolic acids in the cell wall. In this study, we demonstrate the possibility to integrate the enzymatic pretreatment with a simultaneous saccharification and fermentation to achieve ethanol production from grass silage in a one-process step. Achieved yields were about 53 g ethanol per kg silage with the alkaline peroxide pretreatment and 91 g/kg with the enzymatic pretreatment at concentrations of 8.5 and 14.6 g/L, respectively. Furthermore, it was shown that additional supplementation of the fermentation medium with vitamins, trace elements and nutrient salts is not necessary when the press juice is directly used in the fermentation step.
Y1  - 2011
U6  - http://dx.doi.org/10.1002/elsc.201000160
N1  - Special Issue "Bioprocess‐oriented plant design"
VL  - 11
IS  - 4
SP  - 436
EP  - 442
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Roth, Jasmine
A1  - Tippkötter, Nils
T1  - Evaluation of lignocellulosic material for butanol production using enzymatic hydrolysate medium
JF  - Cellulose Chemistry and Technology
N2  - Butanol is a promising gasoline additive and platform chemical that can be readily produced via acetone-butanolethanol (ABE) fermentation from pretreated lignocellulosic materials. This article examines lignocellulosic material from beech wood for ABE fermentation, using Clostridium acetobutylicum. First, the utilization of both C₅₋ (xylose) and C₆₋ (glucose) sugars as sole carbon source was investigated in static cultivation, using serum bottles and synthetic medium. The utilization of pentose sugar resulted in a solvent yield of 0.231 g·g_sugar⁻¹, compared to 0.262 g·g_sugar⁻¹ using hexose. Then, the Organosolv pretreated crude cellulose fibers (CF) were enzymatically decomposed, and the resulting hydrolysate medium was analyzed for inhibiting compounds (furans, organic acids, phenolics) and treated with ionexchangers for detoxification. Batch fermentation in a bioreactor using CF hydrolysate medium resulted in a total solvent yield of 0.20 gABE·g_sugar⁻¹.
Y1  - 2016
VL  - 50
IS  - 3-4
SP  - 405
EP  - 410
PB  - Editura Academiei Romane
CY  - Bukarest
ER  - 
TY  - JOUR
A1  - Salpati, Laurent
A1  - Chu, Xiaoyan
A1  - Chen, Liangfu
A1  - Prasad, Bhagwat
A1  - Dallas, Shannon
A1  - Evers, Raymond
A1  - Mamaril-Fishman, Donna
A1  - Geier, Ethan G.
A1  - Kehler, Jonathan
A1  - Kunta, Jeevan
A1  - Mezler, Mario
A1  - Laplanche, Loic
A1  - Pang, Jodie
A1  - Soars, Matthew G.
A1  - Unadkat, Jashvant D.
A1  - van Waterschoot, Robert A.B.
A1  - Yabut, Jocelyn
A1  - Schinkel, Alfred H.
A1  - Scheer, Nico
A1  - Rode, Anja
T1  - Evaluation of organic anion transporting polypeptide 1B1 and 1B3 humanized mice as a translational model to study the pharmacokinetics of statins
JF  - Drug Metabolism and Disposition
N2  - Organic anion transporting polypeptide (Oatp) 1a/1b knockout and OATP1B1 and -1B3 humanized mouse models are promising tools for studying the roles of these transporters in drug disposition. Detailed characterization of these models will help to better understand their utility for predicting clinical outcomes. To advance this approach, we carried out a comprehensive analysis of these mouse lines by evaluating the compensatory changes in mRNA expression, quantifying the amounts of OATP1B1 and -1B3 protein by liquid chromatography–tandem mass spectrometry, and studying the active uptake in isolated hepatocytes and the pharmacokinetics of some prototypical substrates including statins. Major outcomes from these studies were 1) mostly moderate compensatory changes in only a few genes involved in drug metabolism and disposition, 2) a robust hepatic expression of OATP1B1 and -1B3 proteins in the respective humanized mouse models, and 3) functional activities of the human transporters in hepatocytes isolated from the humanized models with several substrates tested in vitro and with pravastatin in vivo. However, the expression of OATP1B1 and -1B3 in the humanized models did not significantly alter liver or plasma concentrations of rosuvastatin and pitavastatin compared with Oatp1a/1b knockout controls under the conditions used in our studies. Hence, although the humanized OATP1B1 and -1B3 mice showed in vitro and/or in vivo functional activity with some statins, further characterization of these models is required to define their potential use and limitations in the prediction of drug disposition and drug-drug interactions in humans.
Y1  - 2014
U6  - http://dx.doi.org/10.1124/dmd.114.057976
SN  - 1521-009X
VL  - 42
IS  - 8
SP  - 1301
EP  - 1313
PB  - ASPET
CY  - Bethesda, Md.
ER  - 
TY  - JOUR
A1  - Eckert, Alexander
A1  - Rudolph, Tobias
A1  - Guo, Jiaqi
A1  - Mang, Thomas
A1  - Walther, Andreas
T1  - Exceptionally Ductile and Tough Biomimetic Artificial Nacre with Gas Barrier Function
JF  - Advanced Materials
N2  - Synthetic mimics of natural high-performance structural materials have shown great and partly unforeseen opportunities for the design of multifunctional materials. For nacre-mimetic nanocomposites, it has remained extraordinarily challenging to make ductile materials with high stretchability at high fractions of reinforcements, which is however of crucial importance for flexible barrier materials. Here, highly ductile and tough nacre-mimetic nanocomposites are presented, by implementing weak, but many hydrogen bonds in a ternary nacre-mimetic system consisting of two polymers (poly(vinyl amine) and poly(vinyl alcohol)) and natural nanoclay (montmorillonite) to provide efficient energy dissipation and slippage at high nanoclay content (50 wt%). Tailored interactions enable exceptional combinations of ductility (close to 50% strain) and toughness (up to 27.5 MJ m⁻³). Extensive stress whitening, a clear sign of high internal dynamics at high internal cohesion, can be observed during mechanical deformation, and the materials can be folded like paper into origami planes without fracture. Overall, the new levels of ductility and toughness are unprecedented in highly reinforced bioinspired nanocomposites and are of critical importance to future applications, e.g., as barrier materials needed for encapsulation and as a printing substrate for flexible organic electronics.
Y1  - 2018
U6  - http://dx.doi.org/10.1002/adma.201802477
VL  - 30
IS  - 32
SP  - Article number 1802477
PB  - Wiley-VCH
ER  - 
TY  - JOUR
A1  - Siegert, Petra
A1  - McLeish, Michael J.
A1  - Baumann, Martin
A1  - Iding, Hans
A1  - Kneen, Malea M.
A1  - Kenyon, George L.
A1  - Pohl, Martina
T1  - Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida
JF  - Protein engineering, design, and selection : peds
Y1  - 2005
SN  - 1460-213X (E-Journal); 1741-0134 (E-Journal); 0269-2139 (Print); 1741-0126 (Print)
VL  - Vol. 18
IS  - Iss. 7
SP  - 345
EP  - 357
ER  - 
TY  - JOUR
A1  - Ribitsch, D.
A1  - Heumann, S.
A1  - Karl, W.
A1  - Gerlach, J.
A1  - Leber, R.
A1  - Birner-Gruenberger, R.
A1  - Gruber, K.
A1  - Eiteljoerg, I.
A1  - Remler, P.
A1  - Siegert, Petra
A1  - Lange, J.
A1  - Maurer, Karl-Heinz
A1  - Berg, G.
A1  - Guebitz, G. M.
A1  - Schwab, H.
T1  - Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli
JF  - Journal of biotechnology
N2  - A large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. Several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. Encapsulation of cells in alginate beads induced protease production. Stenotrophomonas maltophilia emerged as best performer under washing conditions. For identification of wash-active proteases, four extracellular serine proteases called StmPr1, StmPr2, StmPr3 and StmPr4 were cloned. StmPr2 and StmPr4 were sufficiently overexpressed in E. coli. Expression of StmPr1 and StmPr3 resulted in unprocessed, insoluble protein. Truncation of most of the C-terminal domain which has been identified by enzyme modeling succeeded in expression of soluble, active StmPr1 but failed in case of StmPr3.

From laundry application tests StmPr2 turned out to be a highly wash-active protease at 45 °C. Specific activity of StmPr2 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 17 ± 2 U/mg. In addition we determined the kinetic parameters and cleavage preferences of protease StmPr2.
KW  - Alginate beads
KW  - Stenotrophomonas maltophilia
KW  - Detergent protease
Y1  - 2012
U6  - http://dx.doi.org/10.1016/j.jbiotec.2011.09.025
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - 157
IS  - 1
SP  - 140
EP  - 147
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Takenaga, Shoko
A1  - Schneider, Benno
A1  - Erbay, E.
A1  - Biselli, Manfred
A1  - Schnitzler, Thomas
A1  - Schöning, Michael Josef
A1  - Wagner, Torsten
T1  - Fabrication of biocompatible lab-on-chip devices for biomedical applications by means of a 3D-printing process
JF  - Physica status solidi (a)
N2  - A new microfluidic assembly method for semiconductor-based biosensors using 3D-printing technologies was proposed for a rapid and cost-efficient design of new sensor systems. The microfluidic unit is designed and printed by a 3D-printer in just a few hours and assembled on a light-addressable potentiometric sensor (LAPS) chip using a photo resin. The cell growth curves obtained from culturing cells within microfluidics-based LAPS systems were compared with cell growth curves in cell culture flasks to examine biocompatibility of the 3D-printed chips. Furthermore, an optimal cell culturing within microfluidics-based LAPS chips was achieved by adjusting the fetal calf serum concentrations of the cell culture medium, an important factor for the cell proliferation.
Y1  - 2015
U6  - http://dx.doi.org/10.1002/pssa.201532053
SN  - 1862-6319
VL  - 212
IS  - 6
SP  - 1347
EP  - 1352
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Schiffels, Johannes
A1  - Baumann, Marcus
A1  - Selmer, Thorsten
T1  - Facile analysis of short-chain fatty acids as 4-nitrophenyl esters in complex anaerobic fermentation samples by high performance liquid chromatography
JF  - Journal of Chromatography A. 1218 (2011), H. 34
Y1  - 2011
SN  - 0021-9673
SP  - 5848
EP  - 5851
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - El Moussaoui, Noureddine
A1  - Talbi, Sofian
A1  - Atmane, Ilyas
A1  - Kassmi, Khalil
A1  - Schwarzer, Klemens
A1  - Chayeb, Hamid
A1  - Bachiri, Najib
T1  - Feasibility of a new design of a Parabolic Trough Solar Thermal Cooker (PSTC)
JF  - Solar Energy
N2  - In this article, we describe the structure, the functioning, and the tests of parabolic trough solar thermal cooker (PSTC). This oven is designed to meet the needs of rural residents, including Urban, which requires stable cooking temperatures above 200 °C. The cooking by this cooker is based on the concentration of the sun's rays on a glass vacuum tube and heating of the oil circulate in a big tube, located inside the glass tube. Through two small tubes, associated with large tube, the heated oil, rise and heats the pot of cooking pot containing the food to be cooked (capacity of 5 kg). This cooker is designed in Germany and extensively tested in Morocco for use by the inhabitants who use wood from forests.

During a sunny day, having a maximum solar radiation around 720 W/m2 and temperature ambient around 26 °C, maximum temperatures recorded of the small tube, the large tube and the center of the pot are respectively: 370 °C, 270 °C and 260 °C. The cooking process with food at high (fries, ..), we show that the cooking oil temperature rises to 200 °C, after 1 h of heating, the cooking is done at a temperature of 120 °C for 20 min. These temperatures are practically stable following variations and decreases in the intensity of irradiance during the day. The comparison of these results with those of the literature shows an improvement of 30–50 % on the maximum value of the temperature with a heat storage that could reach 60 min of autonomy. All the results obtained show the good functioning of the PSTC and the feasibility of cooking food at high temperature (>200 °C).
Y1  - 2020
U6  - http://dx.doi.org/10.1016/j.solener.2020.03.079
SN  - 0038-092X
VL  - 201
IS  - Vol. 201 (May 2020)
SP  - 866
EP  - 871
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Wiegand, Sandra
A1  - Voigt, Birgit
A1  - Albrecht, Dirk
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Hecker, Michael
A1  - Daniel, Rolf
A1  - Liesegang, Heiko
T1  - Fermentation stage-dependent adaptations of Bacillus licheniformis during enzyme production
JF  - Microbial Cell Factories
Y1  - 2013
U6  - http://dx.doi.org/10.1186/1475-2859-12-120
SN  - 1475-2859
VL  - 12
SP  - 120
PB  - Biomed Central
CY  - London
ER  - 
TY  - JOUR
A1  - Gerigk, M.
A1  - Maaß, D.
A1  - Takors, Ralf
A1  - Kreutzer, A.
A1  - Wandrey, Christian
A1  - Bongaerts, Johannes
A1  - Wubbolts, Marcel
T1  - Fermentative Herstellung von L-Phenylalanin im Fed-Batch Verfahren mit E. coli unter Einbindung eines integrierten Aufarbeitungsverfahrens
JF  - Chemie-Ingenieur-Technik (CIT)
Y1  - 2000
SN  - 1522-2640 (E-Journal); 0009-286X (Print)
N1  - Printausg. in der Bibliothek vorhanden: 63 ZS 022
VL  - Vol. 72
IS  - Iss. 9
SP  - 926
EP  - 927
ER  - 
TY  - JOUR
A1  - Engel, Mareike
A1  - Bayer, Hendrik
A1  - Holtmann, Dirk
A1  - Tippkötter, Nils
A1  - Ulber, Roland
T1  - Flavin secretion of Clostridium acetobutylicum in a bioelectrochemical system - Is an iron limitation involved?
JF  - Bioelectrochemistry
Y1  - 2019
U6  - http://dx.doi.org/10.1016/j.bioelechem.2019.05.014
SN  - 1567-5394
IS  - In Press, Accepted Manuscript
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Kotter, Michael
A1  - Grazyk, J.
A1  - Geißle, W.
A1  - Roth, A.
T1  - Fließeigenschaften von Keramiken für die Herstellung von Katalysatorträgern / J. Grazyk ; W. Gleißle ; A. Roth ; M. Kotter
JF  - Erdöl & Kohle, Erdgas, Petrochemie : EKEP. 43 (1990), H. 1
Y1  - 1990
SN  - 0014-0058
N1  - 2. ISSN: 0367-0716 ; In der Bibliothek (Jülich) vorhanden unter 63 ZS 034
SP  - 27
EP  - 30
ER  - 
TY  - JOUR
A1  - Gebeshuber, Christoph A.
A1  - Kornauth, Christoph
A1  - Dong, Lihua
A1  - Sierig, Ralph
A1  - Seibler, Jost
A1  - Reiss, Martina
A1  - Tauber, Stefanie
A1  - Bilban, Martin
A1  - Wang, Shijun
A1  - Kain, Renate
A1  - Böhmig, Georg A.
A1  - Moeller, Marcus J.
A1  - Gröne, Hermann-Josef
A1  - Englert, Christoph
A1  - Martinez, Javier
A1  - Kerjaschki, Dontscho
T1  - Focal segmental glomerulosclerosis is induced by microRNA-193a and its downregulation of WT1
JF  - Nature Medicine
Y1  - 2013
U6  - http://dx.doi.org/10.1038/nm.3142
SN  - 1078-8956
VL  - 19
IS  - 4
SP  - 481
EP  - 487
ER  - 
TY  - JOUR
A1  - Kotter, Michael
A1  - Hammon, Ulrich
A1  - Riekert, Lothar
T1  - Formation of ethene and propene from methanol on zeolite ZSM-5 II. Preparation of finished catalysts and operation of a fixed-bed pilot plant / U. Hammon ; M. Kotter ; L. Riekert
JF  - Applied catalysis. 37 (1988)
Y1  - 1988
SN  - 0166-9834
N1  - ISSN der E-Ausg.: 0166-9834
SP  - 155
EP  - 174
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Biselli, Manfred
A1  - Selmer, Thorsten
A1  - Öhlschläger, Peter
A1  - Baumann, Marcus
A1  - Förster, Arnold
A1  - Poghossian, Arshak
T1  - Forschung „zwischen“ den Disziplinen: das Institut für Nano- und Biotechnologien
JF  - Analytik news : das Online-Labormagazin für Labor und Analytik
N2  - "Biologie trifft Mikroelektronik", das Motto des Instituts für Nano- und Biotechnologien (INB) an der FH Aachen, unterstreicht die zunehmende Bedeutung interdisziplinär geprägter Forschungsaktivitäten. Der thematische Zusammenschluss grundständiger Disziplinen, wie die Physik, Elektrotechnik, Chemie, Biologie sowie die Materialwissenschaften, lässt neue Forschungsgebiete entstehen, ein herausragendes Beispiel hierfür ist die Nanotechnologie: Hier werden neue Werkstoffe und Materialien entwickelt, einzelne Nanopartikel oder Moleküle und deren Wechselwirkung untersucht oder Schichtstrukturen im Nanometerbereich aufgebaut, die neue und vorher nicht bekannte Eigenschaften hervorbringen.

Vor diesem Hintergrund bündelt das im Jahre 2006 gegründete INB die an der FH Aachen vorhandenen Kompetenzen von derzeit insgesamt sieben Laboratorien auf den Gebieten der Halbleitertechnik und Nanoelektronik, Nanostrukturen und DNA-Sensorik, der Chemo- und Biosensorik, der Enzymtechnologie, der Mikrobiologie und Pflanzenbiotechnologie, der Zellkulturtechnik, sowie der Roten Biotechnologie synergetisch. In der Nano- und Biotechnologie steckt außergewöhnliches Potenzial! Nicht zuletzt deshalb stellen sich die Forscher der Herausforderung, in diesem Bereich gemeinsam zu forschen und Schnittstellen zu nutzen, um so bei der Gestaltung neuartiger Ideen und Produkte mitzuwirken, die zukünftig unser alltägliches Leben verändern werden.

Im Folgenden werden die verschiedenen Forschungsbereiche kurz zusammenfassend vorgestellt und vorhandene Interaktionen anhand von exemplarisch ausgewählten, aktuellen Forschungsprojekten skizziert.
Y1  - 2012
VL  - Publ. online
PB  - Dr. Beyer Internet-Beratung
CY  - Ober-Ramstadt
ER  - 
TY  - JOUR
A1  - Lassonczyk, Beate
T1  - Fossile Bodenbildung in Nord-Somalia
JF  - Mitteilungen der Deutschen Bodenkundlichen Gesellschaft. 59 (1989), H. 2
Y1  - 1989
SN  - 0343-107x
SP  - 927
EP  - 930
ER  - 
TY  - JOUR
A1  - Schwab, Lukas
A1  - Hojdis, Nils
A1  - Lacayo, Jorge
A1  - Wilhelm, Manfred
T1  - Fourier-Transform Rheology of Unvulcanized, Carbon Black Filled Styrene Butadiene Rubber
JF  - Macromolecular Materials and Engineering
N2  - Rubber materials filled with reinforcing fillers display nonlinear rheological behavior at small strain amplitudes below γ0 < 0.1. Nevertheless, rheological data are analyzed mostly in terms of linear parameters, such as shear moduli (G′, G″), which loose their physical meaning in the nonlinear regime. In this work styrene butadiene rubber filled with carbon black (CB) under large amplitude oscillatory shear (LAOS) is analyzed in terms of the nonlinear parameter I3/1. Three different CB grades are used and the filler load is varied between 0 and 70 phr. It is found that I3/1(φ) is most sensitive to changes of the total accessible filler surface area at low strain amplitudes (γ0 = 0.32). The addition of up to 70 phr CB leads to an increase of I3/1(φ) by a factor of more than ten. The influence of the measurement temperature on I3/1 is pronounced for CB levels above the percolation threshold.
Y1  - 2016
U6  - http://dx.doi.org/10.1002/mame.201500356
SN  - 1439-2054
VL  - 301
IS  - 4
SP  - 457
EP  - 468
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Zientz, Evelyn
A1  - Bongaerts, Johannes
A1  - Unden, Gottfried
T1  - Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system
JF  - Journal of bacteriology
Y1  - 1998
SN  - 1098-5530 (E-Journal); 0021-9193 (Print)
VL  - Vol. 180
IS  - No. 20
SP  - 5421
EP  - 5425
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Al-Kaidy, Huschyar
A1  - Wollny, Steffen
A1  - Ulber, Roland
T1  - Functionalized magnetizable particles for downstream processing in single-use systems
JF  - Chemie Ingenieur Technik
N2  - Biotechnological downstream processing is usually an elaborate procedure, requiring a multitude of unit operations to isolate the target component. Besides the disadvantageous space-time yield, the risks of cross-contaminations and product loss grow fast with the complexity of the isolation procedure. A significant reduction of unit operations can be achieved by application of magnetic particles, especially if these are functionalized with affinity ligands. As magnetic susceptible materials are highly uncommon in biotechnological processes, target binding and selective separation of such particles from fermentation or reactions broths can be done in a single step. Since the magnetizable particles can be produced from iron salts and low priced polymers, a single-use implementation of these systems is highly conceivable. In this article, the principles of magnetizable particles, their synthesis and functionalization are explained. Furthermore, applications in the area of reaction engineering, microfluidics and downstream processing are discussed focusing on established single-use technologies and development potential.
Y1  - 2013
U6  - http://dx.doi.org/10.1002/cite.201200130
VL  - 85
IS  - 1-2: Special Issue: Single-Use Technology
SP  - 76
EP  - 86
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Hoffmann, Ulrich
A1  - Walenzik, C.
A1  - Kögel, J.
T1  - Für Scale-up´s und Betriebsmuster, Projekt Trocknungstechnik an der FH Aachen
JF  - CITplus : das Praxismagazin für Verfahrenstechnik und Apparatebau. 4 (2001)
Y1  - 2001
SN  - 1436-2597
SP  - 24
EP  - 25
ER  - 
TY  - JOUR
A1  - Mang, Thomas
A1  - Roosen, C.
A1  - Ansorge, M.
A1  - Leitner, W.
T1  - Gaining pH-control in water/carbon dioxide biphasic systems / Abstract No. 1038 / Roosen, Ch. ; Ansorge, M. ; Mang, Thomas ; Leitner, W. ; Greiner, L.
JF  - Green solvents for processes : Lake Constance, Friedrichshafen, Germany, 8 - 11 October 2006 ; book of abstracts / DECHEMA e.V.
Y1  - 2006
N1  - DECHEMA, Gesellschaft für Chemische Technik und Biotechnologie ; [Conference] ; ((Friedrichshafen) : 2006.10.08-11)
PB  - DECHEMA
CY  - Frankfurt am Main
ER  - 
TY  - JOUR
A1  - Mang, Thomas
A1  - Roosen, Christoph
A1  - Ansorge-Schumacher, Marion
A1  - Leitner, Walter
T1  - Gaining pH-control in water/carbon dioxide biphasic systems / Roosen, Christoph ; Ansorge-Schumacher, Marion ; Mang, Thomas ; Leitner, Walter ; Greiner, Lasse
JF  - Green Chemistry. 9 (2007)
Y1  - 2007
SN  - 1463-9262
SP  - 455
EP  - 458
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Gäggeler, H. W.
A1  - Jost, D. T.
A1  - Kovacs, J.
T1  - Gas Phase Chromatography Experiments with Bromides of Tantalum and Element 105 / H.W. Gäggeler, D.T. Jost, J. Kovacs, U.W. Scherer, A. Weber, D. Vermeulen, A. Türler, K.E. Gregorich, R.A. Henderson, K.R. Czerwinski, B. Kadkhodayan, D.M. Lee, M. Nurmia, D.
JF  - Radiochimica Acta. 57 (1992)
Y1  - 1992
SN  - 0033-8230
SP  - 93
EP  - 100
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Türler, A.
A1  - Gäggeler, H. W.
A1  - Gregorich, K. E.
T1  - Gas phase chromatography of halides of elements 104 and 105 / A. Türler, H. W. Gäggeler, K. E. Gregorich, H. Barth, W. Brüchle, K. R. Czerwinski, M. K. Gober, N. J. Hannink, R. A. Henderson, D. C. Hoffman, D. T. Jost, C. D. Kacher, B. Kadkhodayan, J. Kova
JF  - Journal of Radioanalytical and Nuclear Chemistry. 160 (1992), H. 2
Y1  - 1992
SN  - 0236-5731
SP  - 327
EP  - 339
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Hör, G.
A1  - Kranert, W. T.
A1  - Maul, F. D.
T1  - Gated Metabolic Positron Emission Tomography (GAPET) of Myocardium: 18F-FDG/PET to optimize Recognition of Myocardial Hibernation / G. Hör, W.T. Kranert, F.D. Maul, O. Schröder,  A. Karimian-Tatriz, O. Geb, R.P. Baum, U.W.  Scherer
JF  - Nuclear Medicine Communications. 19 (1998)
Y1  - 1998
SN  - 0143-3636
SP  - 535
EP  - 545
ER  - 
TY  - JOUR
A1  - Dallas, Shannon
A1  - Salphati, Laurent
A1  - Gomez-Zepeda, David
A1  - Wanek, Thomas
A1  - Chen, Liangfu
A1  - Chu, Xiaoyan
A1  - Kunta, Jeevan
A1  - Mezler, Mario
A1  - Menet, Marie-Claude
A1  - Chasseigneaux, Stephanie
A1  - Declèves, Xavier
A1  - Langer, Oliver
A1  - Pierre, Esaie
A1  - DiLoreto, Karen
A1  - Hoft, Carolin
A1  - Laplanche, Loic
A1  - Pang, Jodie
A1  - Pereira, Tony
A1  - Andonian, Clara
A1  - Simic, Damir
A1  - Rode, Anja
A1  - Yabut, Jocelyn
A1  - Zhang, Xiaolin
A1  - Scheer, Nico
T1  - Generation and Characterization of a Breast Cancer Resistance Protein Humanized Mouse Model
JF  - Molecular Pharmacology
N2  - Breast cancer resistance protein (BCRP) is expressed in various tissues, such as the gut, liver, kidney and blood brain barrier (BBB), where it mediates the unidirectional transport of substrates to the apical/luminal side of polarized cells. Thereby BCRP acts as an efflux pump, mediating the elimination or restricting the entry of endogenous compounds or xenobiotics into tissues and it plays important roles in drug disposition, efficacy and safety. Bcrp knockout mice (Bcrp−/−) have been used widely to study the role of this transporter in limiting intestinal absorption and brain penetration of substrate compounds. Here we describe the first generation and characterization of a mouse line humanized for BCRP (hBCRP), in which the mouse coding sequence from the start to stop codon was replaced with the corresponding human genomic region, such that the human transporter is expressed under control of the murine Bcrp promoter. We demonstrate robust human and loss of mouse BCRP/Bcrp mRNA and protein expression in the hBCRP mice and the absence of major compensatory changes in the expression of other genes involved in drug metabolism and disposition. Pharmacokinetic and brain distribution studies with several BCRP probe substrates confirmed the functional activity of the human transporter in these mice. Furthermore, we provide practical examples for the use of hBCRP mice to study drug-drug interactions (DDIs). The hBCRP mouse is a promising model to study the in vivo role of human BCRP in limiting absorption and BBB penetration of substrate compounds and to investigate clinically relevant DDIs involving BCRP.
Y1  - 2016
U6  - http://dx.doi.org/10.1124/mol.115.102079
SN  - 1521-0111
VL  - 89
IS  - 5
SP  - 492
EP  - 504
PB  - ASPET
CY  - Bethesda, Md.
ER  -