TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Wang, Josepf
A1  - Krause, Robin
A1  - Block, Kirstin
A1  - Musameh, Mustafa
A1  - Mulchandani, Ashok
A1  - Mulchandani, Priti
A1  - Chen, Wilfred
T1  - Dual amperometric-potentiometric biosensor detection system for monitoring organophosphorus neurotoxins
JF  - Analyica Chimica Acta. 469 (2002), H. 2
Y1  - 2002
SN  - 0378-4304
SP  - 197
EP  - 203
ER  - 
TY  - CHAP
A1  - Lei, Yu
A1  - Mulchandani, Priti
A1  - Chen, Wilfred
A1  - Mulchandani, Ashok
T1  - Biosensor for direct determination of fenitrothion and EPN using recombinant Pseudomonas putida JS444 with surface expressed organophosphorus hydrolase. 1. modified clark oxygen electrode
N2  - This paper reports a first microbial biosensor for rapid and cost-effective determination of organophosphorus pesticides fenitrothion and EPN. The biosensor consisted of recombinant PNP-degrading/oxidizing bacteria Pseudomonas putida JS444 anchoring and displaying organophosphorus hydrolase (OPH) on its cell surface as biological sensing element and a dissolved oxygen electrode as the transducer. Surfaceexpressed OPH catalyzed the hydrolysis of fenitrothion and EPN to release 3-methyl-4-nitrophenol and p-nitrophenol, respectively, which were oxidized by the enzymatic machinery of Pseudomonas putida JS444 to carbon dioxide while consuming oxygen, which was measured and correlated to the concentration of organophosphates. Under the optimum operating conditions, the biosensor was able to measure as low as 277 ppb of fenitrothion and 1.6 ppm of EPN without interference from phenolic compounds and other commonly used pesticides such as carbamate pesticides, triazine herbicides and organophosphate pesticides without nitrophenyl substituent. The applicability of the biosensor to lake water was also demonstrated.
KW  - Biosensor
KW  - Organophosphorus
KW  - fenitrothion
KW  - EPN
KW  - biosensor
KW  - Pseudomonas putida
Y1  - 2006
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:a96-opus-1573
ER  -