TY - BOOK A1 - Müller, Thomas A1 - Stöcker, Ernst E. A1 - Lieber, Bettina T1 - Die Organschaft : Körperschaftsteuerrecht, Gewerbesteuerrecht, Umsatzsteuerrecht, Grunderwerbsteuerrecht Y1 - 2014 SN - 978-3-482-44609-2 PB - NWB-Verl. CY - Herne ET - 9. Aufl. ER - TY - JOUR A1 - Alt, Helmut T1 - Wirtschaftlichkeit von Investitionen JF - Elektrizitätswirtschaft Y1 - 1978 SN - 0013-5496 VL - 77 IS - 3 SP - 73 EP - 76 PB - VWEW-Energieverlag CY - Frankfurt/Main ER - TY - JOUR A1 - Alt, Helmut T1 - Isolierte Freileitungen, ein Betriebsmittel für schwierige Trassenführung JF - Elektrizitätswirtschaft Y1 - 1979 SN - 0013-5496 VL - Jg. 78 IS - H. 10 SP - 250 EP - 256 ER - TY - JOUR A1 - Alt, Helmut T1 - Kurzschlußstromberechnung JF - Elektrizitätswirtschaft Y1 - 1980 SN - 0013-5496 VL - Jg. 79 IS - H. 10 SP - 379 EP - 382 ER - TY - JOUR A1 - Alt, Helmut T1 - Wirtschaftlichkeitsberechnung von Investitionen mit dem programmierbaren Taschenrechner JF - 3 R international : RRR ; Rohre, Rohrleitungsbau, Rohrleitungstransport Y1 - 1981 SN - 0340-3386 VL - Jg. 20 IS - H. 10 SP - 561 EP - 565 ER - TY - JOUR A1 - Alt, Helmut T1 - Statische oder dynamische Wirtschaftlichkeitsberechnung bei Investitionen zur Energieeinsparung JF - Elektrowärme international Edition B: Industrielle Elektrowärme : IEW = Industrial applications of electro-heat : EWI ; Zeitschrift für das gesamte Gebiet der Elektrowärme in Industrie, Gewerbe und Haushalt Y1 - 1982 SN - 0340-3521 VL - Jg. 40 IS - H. 3/4 SP - 166 EP - 172 ER - TY - JOUR A1 - Alt, Helmut T1 - Einsatz programmierbarer Rechner im Sondervertragskundenbereich der Gasversorgung JF - Das Gas- und Wasserfach : Ausgabe Gas, Erdgas : GWF Y1 - 1983 SN - 0016-4909 VL - Bd. 124 IS - H. 9 SP - 468 EP - 471 ER - TY - JOUR A1 - Alt, Helmut T1 - Intervallschalter mit getrennt einstellbarer Pulszeit und Pulszahl JF - Elektronik : Fachmedium für industrielle Anwender und Entwickler Y1 - 1978 SN - 0013-5658 VL - 27 IS - H. 14 SP - 76 PB - WEKA Fachmedien CY - Haar ER - TY - CHAP A1 - Alt, Helmut T1 - Analog anzeigende Digitaluhr T2 - FH-Texte ; 36 : Berichte aus dem Fachbereich Elektrotechnik Aachen Y1 - 1983 SP - 98 EP - 104 PB - Fachhochschule Aachen CY - Aachen ER - TY - JOUR A1 - Alt, Helmut T1 - Auswertung von Belastungskennlinien JF - Elektrizitätswirtschaft Y1 - 1983 SN - 0013-5496 VL - Bd. 82 SP - 208 EP - 210 ER - TY - JOUR A1 - Alt, Helmut T1 - Vorteilsausgleich durch Versorgungsunternehmen für Ersatzleistungen Dritter JF - Elektrizitätswirtschaft Y1 - 1985 SN - 0013-5496 VL - Jg. 104 SP - 440 EP - 443 ER - TY - JOUR A1 - Alt, Helmut T1 - Multiplikativ oder additiv? Diskussionsbeitrag zu Preisanpassungsklauseln in der Gaswirtschaft JF - Energiewirtschaftliche Tagesfragen : et ; Zeitschrift für Energiewirtschaft, Recht, Technik und Umwelt Y1 - 1985 SN - 0013-743X VL - Jg. 35 SP - 609 EP - 611 ER - TY - JOUR A1 - Alt, Helmut T1 - Steuersenkungsgesetz-Auswirkungen und Berechnung JF - Elektrizitätswirtschaft Y1 - 1986 SN - 0013-5496 VL - Jg. 85 SP - 238 EP - 242 ER - TY - JOUR A1 - Alt, Helmut T1 - Dialoggesteuerte harmonische Analyse mit dem HP 41 JF - Elektrizitätswirtschaft Y1 - 1986 SN - 0013-5496 VL - Bd. 85 IS - H. 4 SP - 139 EP - 142 ER - TY - JOUR A1 - Alt, Helmut T1 - Dialoggesteuerte harmonische Analyse mit dem HP 41 JF - Elektrowärme international Edition B : Industrielle Elektrowärme : IEW = Industrial applications of electro-heat : EWI ; Zeitschr. für das gesamte Gebiet der Elektrowärme in Industrie, Gewerbe und Haushalt Y1 - 1986 SN - 0340-3521 VL - Jg. 44 IS - H. 6 SP - 283 EP - 287 ER - TY - JOUR A1 - Alt, Helmut T1 - Dialoggesteuerte Spannungs- und Belastungsberechnung im Netz eines Elektrizitätsversorgungsunternehmens JF - Elektrizitätswirtschaft Y1 - 1986 SN - 0013-5496 VL - Bd. 85 IS - H. 23 SP - 906 EP - 910 ER - TY - JOUR A1 - Alt, Helmut T1 - Effektivzinsberechnung bei industriellen oder gewerblichen Tilgungsdarlehen JF - Elektrizitätswirtschaft Y1 - 1987 SN - 0013-5496 VL - Bd. 86 SP - 592 EP - 595 ER - TY - JOUR A1 - Alt, Helmut T1 - Die wichtigsten Kontoführungsmodelle JF - Frankfurter Zeitung : Blick durch die Wirtschaft : Zeitung für Finanzen, Steuern, Recht und Management Y1 - 1988 IS - Nr. 240 vom 13.12.1988 SP - 3 ER - TY - JOUR A1 - Alt, Helmut T1 - Steuerreformgesetz 1990 JF - Elektrizitätswirtschaft Y1 - 1989 SN - 0013-5496 VL - Bd. 88 IS - H. 1/2 SP - 78 EP - 83 ER - TY - JOUR A1 - Alt, Helmut T1 - Steuersenkungs-Erweiterungsgesetz 1988 zur Gesamtentlastung der Einkommensteuer JF - Betriebs-Berater : BB ; Zeitschrift für Recht und Wirtschaft Y1 - 1987 SN - 0340-7918 VL - Vol. 42 IS - H. 32 SP - 2207 EP - 2208 ER - TY - JOUR A1 - Alt, Helmut T1 - Steuerreformgesetz 1990 JF - Elektrizitätswirtschaft Y1 - 1989 SN - 0947-0581 (E-Journal); 0340-7918 (Print) VL - Jg. 88 IS - H. 2 SP - 120 EP - 121 ER - TY - JOUR A1 - Alt, Helmut T1 - Energietarifstruktur in Ostdeutschland JF - Energiewirtschaftliche Tagesfragen : et ; Zeitschrift für Energiewirtschaft, Recht, Technik und Umwelt Y1 - 1990 SN - 0013-743X VL - Bd. 40 IS - H. 11 SP - 782 EP - 788 ER - TY - JOUR A1 - Alt, Helmut T1 - Effektivzins und APR JF - Die Bank : Zeitschrift für Bankpolitik und Bankpraxis Y1 - 1991 SN - 0342-3182 N1 - Printausg. in der Bibliothek vorhanden: 43 Z 227 VL - Jg. 31 IS - H. 4 SP - 230 EP - 231 ER - TY - JOUR A1 - Alt, Helmut T1 - Möglichkeiten der CO2-Minderung "alternativ" betrachtet JF - Etz : Elektrotechnik & Automation Y1 - 1992 SN - 0170-1711 VL - Bd. 113 IS - H. 6-7 SP - 400 ER - TY - JOUR A1 - Alt, Helmut T1 - Netzimpedanz- und Oberschwingungsberechnung in Netzen der öffentlichen Stromversorgung JF - Elektrizitätswirtschaft Y1 - 1995 SN - 0013-5496 VL - Bd. 94 IS - H. 4 SP - 166 EP - 174 ER - TY - CHAP A1 - Terstegge, Andreas A1 - Weber, S. A1 - Herzog, H. A1 - Müller-Gärtner, H. W. A1 - Halling, H. T1 - Design and implementation aspects of a 3D reconstruction algorithm for the Jülich TierPET system T2 - 1997 International Meeting on Fully ThreeDimensional Image Reconstruction in Radiology and Nuclear Medicine : 3D97 Y1 - 1997 SP - 170 EP - 173 ER - TY - CHAP A1 - Weber, S. A1 - Terstegge, Andreas A1 - Engels, R. A1 - Herzog, H. A1 - Reinartz, R. A1 - Reinhart, P. A1 - Rongen, F. A1 - Müller-Gärtner, H. W. A1 - Halling, H. T1 - The KFA TierPET: performance characteristics and measurements T2 - Conference record / 1996 IEEE Nuclear Science Symposium [and Medical Imaging], November 2 - 9, 1996, Anaheim, California ; vol. 2 Y1 - 1996 SN - 0-7803-3534-1 SN - 1082-3654 SP - 1117 EP - 1119 PB - IEEE CY - Piscataway, NJ ER - TY - CHAP A1 - Weber, S. A1 - Terstegge, Andreas A1 - Halling, H. A1 - Herzog, H. A1 - Reinartz, R. A1 - Reinhart, P. A1 - Rongen, F. A1 - Müller-Gärtner, H.-W. T1 - The design of an animal PET: flexible geometry for achieving optimal spatial resolution or high sensitivity T2 - Conference record / 1995 IEEE Nuclear Science Symposium and Medical Imaging, October 21 - 28, 1995, San Francisco ; vol. 2 Y1 - 1995 SN - 078033180X ; 0780331818 ; 0780331826 SP - 1002 EP - 1005 PB - IEEE CY - Piscataway, NJ ER - TY - JOUR A1 - Wang, Ren-Qi A1 - Druckenmüller, Katharina A1 - Elbers, Gereon A1 - Guenther, Klaus A1 - Croué, Jean-Philippe T1 - Analysis of aquatic-phase natural organic matter by optimized LDI-MS method JF - Journal of mass spectrometry N2 - The composition and physiochemical properties of aquatic-phase natural organic matter (NOM) are most important problems for both environmental studies and water industry. Laser desorption/ionization (LDI) mass spectrometry facilitated successful examinations of NOM, as humic and fulvic acids in NOM are readily ionized by the nitrogen laser. In this study, hydrophobic NOMs (HPO NOMs) from river, reservoir and waste water were characterized by this technique. The effect of analytical variables like concentration, solvent composition and laser energy was investigated. The exact masses of small molecular NOM moieties in the range of 200–1200 m/z were determined in reflectron mode. In addition, spectra of post-source-decay experiments in this range showed that some compounds from different natural NOMs had the same fragmental ions. In the large mass range of 1200–15 000 Da, macromolecules and their aggregates were found in HPO NOMs from natural waters. Highly humic HPO exhibited mass peaks larger than 8000 Da. On the other hand, the waste water and reservoir water mainly had relatively smaller molecules of about 2000 Da. The LDI-MS measurements indicated that highly humic river waters were able to form large aggregates and membrane foulants, while the HPO NOMs from waste water and reservoir water were unlikely to form large aggregates. Copyright © 2014 John Wiley & Sons, Ltd. Y1 - 2014 U6 - https://doi.org/10.1002/jms.3321 SN - 1096-9888 VL - 49 IS - 2 SP - 154 EP - 160 PB - Wiley CY - Bognor Regis ER - TY - BOOK A1 - Fredebeul-Krein, Markus A1 - Koch, Walter A. S. A1 - Kulessa, Margareta A1 - Sputek, Agnes T1 - Grundlagen der Wirtschaftspolitik Y1 - 2014 SN - 978-3-8252-8556-2 PB - UVK Verl.-Ges. CY - Konstanz [u.a.] ET - 4. Aufl. ER - TY - JOUR A1 - Sawada, Kazuaki A1 - Nakazawa, Hirokazu A1 - Takenaga, Shoko A1 - Hizawa, Takeshi A1 - Futagawa, Masato A1 - Dasai, Fumihiro A1 - Sakurai, Takashi A1 - Okumura, Koichi A1 - Hattori, Toshiaki A1 - Ishida, Makoto T1 - Multimodal bioimage sensor JF - IEICE transactions on fundamentals of electronics, communidations and computer sciences N2 - To visualize the biochemical distribution two-dimensionally, we invented a solid-state-type ion image sensor that indicates the chemical activity of solutions and cells. The device, which consists of a CCD array covered with a functionalized membrane to detect charge accumulation, is highly sensitive to changes in the concentration and two-dimensional distribution of ions and biomaterials. Y1 - 2014 U6 - https://doi.org/10.1587/transfun.E97.A.726 SN - 0916-8508 (Print) ; 1745-1337 (Online) VL - E97-A (2014) IS - 3 SP - 726 EP - 733 PB - IEICE CY - Tokyo ER - TY - JOUR A1 - Heine, A. A1 - Herrmann, G. A1 - Selmer, Thorsten A1 - Terwesten, F. A1 - Buckel, W. A1 - Reuter, K. T1 - High resolution crystal structure of clostridium propionicum β-Alanyl-CoA:Ammonia Lyase, a new member of the "Hot Dog Fold" protein superfamily JF - Proteins N2 - Clostridium propionicum is the only organism known to ferment β-alanine, a constituent of coenzyme A (CoA) and the phosphopantetheinyl prosthetic group of holo-acyl carrier protein. The first step in the fermentation is a CoA-transfer to β-alanine. Subsequently, the resulting β-alanyl-CoA is deaminated by the enzyme β-alanyl-CoA:ammonia lyase (Acl) to reversibly form ammonia and acrylyl-CoA. We have determined the crystal structure of Acl in its apo-form at a resolution of 0.97 Å as well as in complex with CoA at a resolution of 1.59 Å. The structures reveal that the enyzme belongs to a superfamily of proteins exhibiting a so called “hot dog fold” which is characterized by a five-stranded antiparallel β-sheet with a long α-helix packed against it. The functional unit of all “hot dog fold” proteins is a homodimer containing two equivalent substrate binding sites which are established by the dimer interface. In the case of Acl, three functional dimers combine to a homohexamer strongly resembling the homohexamer formed by YciA-like acyl-CoA thioesterases. Here, we propose an enzymatic mechanism based on the crystal structure of the Acl·CoA complex and molecular docking. Proteins 2014; 82:2041–2053. © 2014 Wiley Periodicals, Inc. Y1 - 2014 U6 - https://doi.org/10.1002/prot.24557 SN - 1097-0134 (E-Journal); 0887-3585 (Print) VL - 82 IS - 9 SP - 2041 EP - 2053 PB - Wiley-Liss CY - New York ER - TY - CHAP A1 - Starke, Günther T1 - Robotertechnik für individuelle Automatisierungslösungen und unterschiedlichste Prozessanwendungen : 17. Internationale Fachmesse „Schweißen & Schneiden 2009“ – Stand und Entwicklungstendenzen T2 - Schweissen und Schneiden Y1 - 2009 SN - 0036-7184 (Print) SP - N 13 (nach Seite 716) PB - DVS - Deutscher Verband für Schweißen und Verwandte Verfahren e.V. ER - TY - JOUR A1 - Starke, Günther A1 - Ahlbehrendt, Norbert A1 - Bischoff, Rainer A1 - Melzer, Harald A1 - Sieber, Sven A1 - Senk, Burkhard A1 - Kramarczyk, Peter T1 - RoboTOOL. Technologien für den ortsflexiblen Einsatz von Robotern bei temporären Arbeitsaufgaben in der Fertigung JF - wt Werkstattstechnik online Y1 - 2009 SN - 1436-4980 VL - Bd. 99 IS - H. 9 SP - 661 EP - 670 ER - TY - BOOK A1 - Nebgen, Nikolaus A1 - Peterson, Leif Arne T1 - Holzbau kompakt nach Eurocode 5 Y1 - 2014 SN - 978-3-410-22414-3 PB - Beuth CY - Berlin ET - 4. Aufl. ER - TY - JOUR A1 - Takenaga, Shoko A1 - Biselli, Manfred A1 - Schnitzler, Thomas A1 - Öhlschläger, Peter A1 - Wagner, Torsten A1 - Schöning, Michael Josef T1 - Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis–Menten-like kinetics for cell culturing JF - Physica status solidi A : Applications and materials science N2 - The metabolic activity of Chinese hamster ovary (CHO) cells was observed using a light-addressable potentiometric sensor (LAPS). The dependency toward different glucose concentrations (17–200 mM) follows a Michaelis–Menten kinetics trajectory with Kₘ = 32.8 mM, and the obtained Kₘ value in this experiment was compared with that found in literature. In addition, the pH shift induced by glucose metabolism of tumor cells transfected with the HPV-16 genome (C3 cells) was successfully observed. These results indicate the possibility to determine the tumor cells metabolism with a LAPS-based measurement device. Y1 - 2014 U6 - https://doi.org/10.1002/pssa.201330464 SN - 1521-396X (E); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print) VL - 211 IS - 6 SP - 1410 EP - 1415 PB - Wiley-VCH CY - Weinheim ER - TY - CHAP A1 - Schmidt, Herbert T1 - Angst vor Zukunft, Lust auf Zukunft T2 - Re|peat-Jahrbuch Treasury und Private Banking : Produkte, Märkte und Strategien zum Nachschlagen und Verstehen Y1 - 2014 SP - 255 EP - 269 PB - Roland Eller CY - Potsdam ER - TY - CHAP A1 - Mandelartz, Johannes T1 - Der Aluminium-Sekundärstoffstrom in Deutschland unter transporttechnischen Gesichtspunkten : Vortrag T2 - Rohstofftechnik im Wandel : 26./27. November 1998 ; Tagungsband / Rheinisch-Westfälische Technische Hochschule Aachen. Aachener Umwelttage der Fakultät für Bergbau, Hüttenwesen und Geowissenschaften Y1 - 1998 SP - V.1,1 EP - V.1,21 ER - TY - JOUR A1 - Funke, Harald A1 - Dickhoff, J. A1 - Keinz, Jan A1 - Anis, H. A. A1 - Parente, A. A1 - Hendrick, P. T1 - Experimental and numerical study of the micromix combustion principle applied for hydrogen and hydrogen-rich syngas as fuel with increased energy density for industrial gas turbine applications JF - Energy procedia N2 - The Dry Low NOx (DLN) Micromix combustion principle with increased energy density is adapted for the industrial gas turbine APU GTCP 36-300 using hydrogen and hydrogen-rich syngas with a composition of 90%-Vol. hydrogen (H₂) and 10%-Vol. carbon-monoxide (CO). Experimental and numerical studies of several combustor geometries for hydrogen and syngas show the successful advance of the DLN Micromix combustion from pure hydrogen to hydrogen-rich syngas. The impact of the different fuel properties on the combustion principle and aerodynamic flame stabilization design laws, flow field, flame structure and emission characteristics is investigated by numerical analysis using a hybrid Eddy Break Up combustion model and validated against experimental results. Y1 - 2014 U6 - https://doi.org/10.1016/j.egypro.2014.12.201 SN - 1876-6102 (E-Journal) IS - 61 SP - 1736 EP - 1739 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Oberländer, Jan A1 - Kirchner, Patrick A1 - Boyen, Hans-Gerd A1 - Schöning, Michael Josef T1 - Detection of hydrogen peroxide vapor by use of manganese(IV) oxide as catalyst for calorimetric gas sensors JF - Physica status solidi A: Applications and materials science N2 - In this work, the catalyst manganese(IV) oxide (MnO2), of calorimetric gas sensors (to monitor the sterilization agent vaporized hydrogen peroxide) has been investigated in more detail. Chemical analyses by means of X-ray-induced photoelectron spectroscopy have been performed to unravel the surface chemistry prior and after exposure to hydrogen peroxide vapor at elevated temperature, as applied in the sterilization processes of beverage cartons. The surface characterization reveals a change in oxidation states of the metal oxide catalyst after exposure to hydrogen peroxide. Additionally, a cleaning effect of the catalyst, which itself is attached to the sensor surface by means of a polymer interlayer, could be observed. Y1 - 2014 U6 - https://doi.org/10.1002/pssa.201330359 SN - 1521-396X (E-Journal); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print) VL - 211 IS - 6 SP - 1372 EP - 1376 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Frings, Michael T1 - Das Gesetz gegen unseriöse Geschäftspraktiken JF - NWB : NWB Steuer- und Wirtschaftsrecht Y1 - 2014 SN - 0028-3460 (Print) IS - 11 SP - 769 EP - 775 PB - NWB-Verlag CY - Herne ER - TY - CHAP A1 - Starke, Günther A1 - Kunkel, Thomas A1 - Hahn, Daniel T1 - Flexible collaboration and control of heterogeneous mechatronic devices and systems by means of an event-driven, SOA-based automation concept T2 - 2013 IEEE International Conference on Industrial Technologies (ICIT-13) Y1 - 2013 SN - 978-1-4673-4567-5 ; 978-1-4673-4569-9 SP - 1982 EP - 1987 PB - IEEE CY - Piscataway, NJ ER - TY - JOUR A1 - Frings, Michael T1 - Neuregelung des Verbraucherschutzes – Umsetzung der EU-Verbraucherrechterichtlinie JF - NWB : NWB Steuer- und Wirtschaftsrecht Y1 - 2014 SN - 0028-3460 (Print) IS - 14 SP - 1003 EP - 1018 PB - NWB-Verlag CY - Herne ER - TY - JOUR A1 - Fuest, Matthias A1 - Kotliar, Konstantin A1 - Walter, Peter A1 - Plange, Niklas T1 - Monitoring intraocular pressure changes after intravitreal Ranibizumab injection using rebound tonometry JF - Ophthalmic and physiological optics Y1 - 2014 U6 - https://doi.org/10.1111/opo.12134 SN - 1475-1313 (E-Journal); 0275-5408 (Print) N1 - Special Issue: The Ageing Visual System VL - 34 IS - 4 SP - 438 EP - 444 PB - Wiley-Blackwell CY - Oxford ER - TY - CHAP A1 - Kern, Alexander T1 - Die neue DIN EN 62305-2 (VDE 0185-305-2) Ed.2:2013-02 - Änderungen zur Ed.l und zugehörige Beiblätter T2 - 10. VDE, VBB-Blitzschutztagung : Vorträge der 10. VDE, ABB-Fachtagung vom 24. bis 25. Oktober 2013 in Neu-Ulm. (VDE-Fachbericht ; 70) Y1 - 2013 SN - 978-3-8007-3540-2 SP - 9 EP - 16 PB - VDE-Verl. CY - Berlin ER - TY - CHAP A1 - Scheibe, K. A1 - Kern, Alexander T1 - Stand der Normung für Überspannungsschutzgeräte T2 - 10. VDE, VBB-Blitzschutztagung : Vorträge der 10. VDE, ABB-Fachtagung vom 24. bis 25. Oktober 2013 in Neu-Ulm. (VDE-Fachbericht ; 70) Y1 - 2013 SN - 978-3-8007-3540-2 SP - 23 EP - 27 PB - VDE-Verl. CY - Berlin ER - TY - JOUR A1 - Guo, Yuanyuan A1 - Miyamoto, Ko-ichiro A1 - Wagner, Torsten A1 - Schöning, Michael Josef A1 - Yoshinobu, Tatsuo T1 - Theoretical study and simulation of light-addressable potentiometric sensors JF - Physica status solidi (A) : applications and materials N2 - The light-addressable potentiometric sensor (LAPS) is a semiconductor-based potentiometric sensor using a light probe with an ability of detecting the concentration of biochemical species in a spatially resolved manner. As an important biomedical sensor, research has been conducted to improve its performance, for instance, to realize high-speed measurement. In this work, the idea of facilitating the device-level simulation, instead of using an equivalent-circuit model, is presented for detailed analysis and optimization of the performance of the LAPS. Both carrier distribution and photocurrent response have been simulated to provide new insight into both amplitude-mode and phase-mode operations of the LAPS. Various device parameters can be examined to effectively design and optimize the LAPS structures and setups for enhanced performance. Y1 - 2014 U6 - https://doi.org/10.1002/pssa.201330354 SN - 0031-8965 VL - 211 IS - 6 SP - 1467 EP - 1472 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Teschers, Reimar A1 - Markhöfer, Jörg A1 - Mandelartz, Johannes T1 - Aluminiumrückführung aus dem Automobilbereich JF - Erzmetall : Rohstoffgewinnung, Verarbeitung, Recycling, Umwelttechnik Y1 - 1999 SN - 0044-2658 VL - Bd. 52 IS - Nr. 10 SP - 533 EP - 540 ER - TY - JOUR A1 - Mandelartz, Johannes A1 - Teschers, Reimar A1 - Schumacher, Thomas T1 - Aluminium-Einsatz im Verkehrsbau JF - Erzmetall : Rohstoffgewinnung, Verarbeitung, Recycling, Umwelttechnik Y1 - 1999 SN - 0044-2658 VL - Bd. 52 IS - Nr. 7-8 SP - 411 EP - 418 ER - TY - CHAP A1 - Petry, Martin A1 - Mandelartz, Johannes T1 - Wertorientiertes Instandhaltungsmanagement T2 - Maintenance ideas : von der technischen Diagnose zur betriebswirtschaftlichen Prognose ; Tagung Aachen, 16. und 17. Mai 2000. (VDI-Berichte. 1554) Y1 - 2000 SN - 3-18-091554-4 SP - 627 EP - 647 PB - VDI-Verl. CY - Düsseldorf ER - TY - JOUR A1 - Smith, Walker O. A1 - Baumann, Marcus A1 - Wilson, David L. A1 - Aletsee, Ludwig T1 - Phytoplankton biomass and productivity in the marginal ice zone of the Fram Strait during summer 1984 JF - Journal of geophysical research Y1 - 1987 SN - 2156-2202 (E-Journal); 2169-9291 (E-Journal); 0148-0227 (Print); 2169-9275 (Print) VL - Vol. 92 IS - Iss. C7 SP - 6777 EP - 6786 ER - TY - JOUR A1 - Jahnke, J. A1 - Baumann, Marcus T1 - Differentiation between Phaeocystis pouchetii (Har.) Lagerheim and Phaeocystis globosa Scherffel JF - Hydrobiological bulletin Y1 - 1987 SN - 0165-1404 (Print); 1573-5125 (E-Journal) VL - Vol. 21 IS - Iss. 2 SP - 141 EP - 147 ER - TY - CHAP A1 - Baumann, Marcus ED - Hempel, G. T1 - Primary production and phytoplankton growth in the marginal ice zone of the Fram Strait and the interpretation of the field data with autecological experiments (Abstract) T2 - Oceanography and biology of Arctic seas : selected papers from the ICES Symposium on Marine Sciences of the Arctic and Subarctic Regions, held in Santander, 28 - 30 September 1987. (Rapports et procès-verbaux des réunions / Conseil International pour l'Exploration de la Mer ; 188) Y1 - 1989 SP - 128 PB - International Council for the Exploration of the Sea CY - Copenhagen ER - TY - JOUR A1 - Aletsee, Ludwig A1 - Baumann, Marcus T1 - A laboratory incubator equipped with facilities to automatically simulate natural irradiance JF - Boletim do Instituto Oceanográfico / Universidade de São Paulo Y1 - 1991 SN - 1982-436X; 0080-6331 VL - Vol. 39 IS - No. 2 SP - 155 EP - 159 ER - TY - JOUR A1 - Tüg, Helmut A1 - Baumann, Marcus T1 - Problems of UV-B radiation measurements in biological research : critical remarks on current techniques and suggestions for improvements JF - Geophysical research letters Y1 - 1994 SN - 1944-8007 (E-Journal); 0094-8276 (Print) VL - Vol. 21 IS - Iss. 8 SP - 689 EP - 692 ER - TY - JOUR A1 - Medlin, L. K. A1 - Lange, M. A1 - Baumann, Marcus T1 - Genetic differentiation among three colony-forming species of Phaeocystis : further evidence for the phylogeny of the Prymnesiophyta JF - Phycologia Y1 - 1994 SN - 0031-8884 VL - Vol. 33 IS - Iss. 3 SP - 199 EP - 212 ER - TY - CHAP A1 - Medlin, L. K. A1 - Barker, G. L. A. A1 - Baumann, Marcus A1 - Hayes, P. K. T1 - Molecular biology and systematics T2 - The Haptophyte Algae (Special volume / Systematics Association : 51) Y1 - 1994 SN - 0-19-857772-9 SP - 393 EP - 411 PB - Clarendon Press CY - Oxford ER - TY - JOUR A1 - Tüg, Helmut A1 - Baumann, Marcus T1 - Reply to the comments by R.L. McKenzie and P.V. Johnston on our paper “Problems of UV-B radiation measurements in biological research: Critical Remarks on current techniques and suggestions for improvements” JF - Geophysical research letters Y1 - 1995 SN - 1944-8007 (E-Journal); 0094-8276 (Print) VL - Vol. 22 IS - Iss. 9 SP - 1159 EP - 1160 ER - TY - CHAP A1 - Brandini, Frederico P. A1 - Baumann, Marcus T1 - The potential role of melted 'brown ice' as sources of chelators and ammonia to the surface waters of the Weddell Sea, Antarctica T2 - Proceedings of the NIPR Symposium on Polar Biology : 10 Y1 - 1997 SN - 0914-563X SP - 1 EP - 13 CY - Tokyo ER - TY - JOUR A1 - Baumann, Marcus T1 - Algenblüten & Treibhauseffekt : Wechselwirkungen mit anthropogenen Aktivitäten JF - Wechselwirkung : Wissenschaft & vernetztes Denken Y1 - 1996 SN - 0172-1623 VL - 18 IS - 81 SP - 6 EP - 11 ER - TY - JOUR A1 - Petry, Martin A1 - Mandelartz, Johannes T1 - Wertorientiertes Instandhaltungsmanagement JF - Erzmetall : Rohstoffgewinnung, Verarbeitung, Recycling, Umwelttechnik Y1 - 2001 SN - 0044-2658 VL - Bd. 54 IS - H. 3 SP - 134 EP - 142 ER - TY - CHAP A1 - Mandelartz, Johannes A1 - Teschers, Reimar A1 - Schumacher, Thomas T1 - Aluminium-Einsatz im Verkehrssektor T2 - Resource-orientated analysis of metallic raw materials : findings of CRC 525 for aluminium. (Reihe Materie und Material ; 17.) Y1 - 2003 SN - 3-89336-342-4 SP - 7.1 EP - 7.11 PB - Forschungszentrum Jülich CY - Jülich ER - TY - CHAP A1 - Teschers, Reimar A1 - Markhöfer, Jörg A1 - Mandelartz, Johannes T1 - Die Aluminiumrückführung aus dem Automobilbereich T2 - Resource-orientated analysis of metallic raw materials : findings of CRC 525 for aluminium. (Reihe Materie und Material ; 17.) Y1 - 2003 SN - 3-89336-342-4 SP - 7.1 EP - 7.12 PB - Forschungszentrum Jülich CY - Jülich ER - TY - CHAP A1 - Mandelartz, Johannes T1 - VDI-Richtlinien und Standards zu RFID T2 - RFID in der Instandhaltung. (Forum Vision Instandhaltung) Y1 - 2007 SN - 978-3-89957-061-8 SP - 169 EP - 179 PB - Verl. Praxiswissen CY - Dortmund ER - TY - CHAP A1 - Schmidt, Herbert T1 - Finanzierungspartnerwahl durch mittelständische Unternehmen T2 - Innovation durch Kooperation : wie der Mittelstand durch Zusammenarbeit mit den Hochschulen seine Wettbewerbsfähigkeit steigert ; Festschrift für Prof. Dr. rer. nat. Johannes Gartzen / Thomas Gartzen, Ute Gartzen (Hrsg.) Y1 - 2013 SN - 978-3-86359-136-6 SP - 163 EP - 166 PB - Apprimus Verlag CY - Aachen ER - TY - CHAP A1 - Fateri, Miranda A1 - Gebhardt, Andreas A1 - Khosravi, Maziar T1 - Experimental investigation of selective laser melting of lunar regolith for in-situ applications T2 - ASME 2013 International Mechanical Engineering Congress and Exposition : San Diego, California, USA, November 15–21, 2013. Vol. 2A: Advanced manufacturing Y1 - 2013 SN - 978-0-7918-5618-5 SP - V02AT02A008 PB - ASME ER - TY - BOOK A1 - Siedenbiedel, Georg T1 - Corporate Compliance : Grundelemente der strukturellen Integration von Compliance-Konzepten Y1 - 2014 SN - 3-482-65131-0 SN - 978-3-482-65131-1 PB - NWB-Verl. CY - Herne ER - TY - BOOK A1 - Hüning, Felix T1 - The fundamentals of electrical engineering for mechatronics Y1 - 2014 SN - 978-3-11-034991-7 (Druckausg.) SN - 978-3-11-030840-2 (E-Book) PB - de Gruyter CY - Berlin ER - TY - JOUR A1 - Schirra, Julian A1 - Watmuff, Jonathan A1 - Bauschat, J.-Michael T1 - Highly non-planar lifting systems: a relative assessment of existing potential-methodologies to accurately estimate the induced drag JF - 32nd AIAA Applied Aerodynamics Conference 2014 : June, 16-20 2014, Atlanta, Ga. Y1 - 2014 SN - 978-1-62410-288-2 U6 - https://doi.org/10.2514/6.2014-2988 SP - Publ. online ER - TY - CHAP A1 - Hoepner, Gert A1 - Schminke, Lutz H. T1 - Dialog-Marketing – 2 in 1 – mehr als 3-fach erfolgreich T2 - Marketing in Forschung und Praxis : [Jubiläumsausgabe zum 40-jährigen Bestehen der Arbeitsgemeinschaft für Marketing] / hrsg. von Günter Hofbauer ... Y1 - 2013 SN - 978-3-942171-98-4 SP - 375 EP - 396 PB - Uni-Ed. CY - Berlin ER - TY - CHAP A1 - Funke, Harald A1 - Keinz, Jan A1 - Börner, Sebastian A1 - Haj Ayed, A. A1 - Kusterer, K. A1 - Tekin, N. A1 - Kazari, M. A1 - Kitajima, J. A1 - Horikawa, A. A1 - Okada, K. ED - Song, Seung Jin T1 - Experimental and numerical characterization of the dry low NOx micromix hydrogen combustion principle at increased energy density for industrial hydrogen gas turbine applications T2 - Combustion, fuels and emissions : proceedings of the ASME Turbo Expo: Turbine Technical Conference and Exposition - 2013 ; June 3 - 7, 2013, San Antonio, Texas, USA ; vol. 1 Y1 - 2013 SN - 978-0-7918-5510-2 N1 - Paper No: GT2013-94771 SP - V001T04A055 PB - ASME CY - New York, NY ER - TY - THES A1 - Werner, Frederik T1 - Development of light-addressable potentiometric sensor systems and their applications in biotechnological environments Y1 - 2014 N1 - Marburg, Univ., Diss., 2014 ER - TY - CHAP A1 - Schreiber, Marc A1 - Hirtbach, Stefan A1 - Kraft, Bodo A1 - Steinmetzler, Andreas ED - Kowalewski, Stefan T1 - Software in the city: visual guidance through large scale software projects T2 - Software Engineering 2013 : Fachtagung des GI-Fachbereichs Softwaretechnik, 26. Februar-1. März 2013 in Aachen. (GI-Edition ; 213) Y1 - 2013 SN - 978-3-88579-607-7 ; 978-3-88579-609-1 SP - 213 EP - 224 PB - Ges. für Informatik CY - Bonn ER - TY - CHAP A1 - Schreiber, Marc A1 - Barkschat, Kai A1 - Kraft, Bodo T1 - Using Continuous Integration to organize and monitor the annotation process of domain specific corpora T2 - 5th International Conference on Information and Communication Systems (ICICS) : 1-3 April 2014, Irbid, Jordanien Y1 - 2014 SN - 978-1-4799-3022-7 U6 - https://doi.org/10.1109/IACS.2014.6841958 SP - 1 EP - 6 ER - TY - JOUR A1 - Riedel, Marc A1 - Kartchemnik, Julia A1 - Schöning, Michael Josef A1 - Lisdat, Fred T1 - Impedimetric DNA detection – steps forward to sensorial application JF - Analytical chemistry N2 - This study describes a label-free impedimetric sensor based on short ssDNA recognition elements for the detection of hybridization events. We concentrate on the elucidation of the influence of target length and recognition sequence position on the sensorial performance. The impedimetric measurements are performed in the presence of the redox system ferri-/ferrocyanide and show an increase in charge transfer resistance upon hybridization of ssDNA to the sensor surface. Investigations on the impedimetric signal stability demonstrate a clear influence of the buffers used during the sensor preparation and the choice of the passivating mercaptoalcanol compound. A stable sensor system has been developed, enabling a reproducible detection of 25mer target DNA in the low nanomolar range. After hybridization, a sensor regeneration can be reached with deionized water by adjustment of effective convection conditions, ensuring a sensor reusability. By investigations of longer targets with overhangs exposed to the solution, we can demonstrate applicability of the impedimetric detection for longer ssDNA. However, a decreasing charge transfer resistance change (ΔRct) is found by extending the overhang. As a strategy to increase the impedance change for longer target strands, the position of the recognition sequence can be designed in a way that a small overhang is exposed to the electrode surface. This is found to result in an increase in the relative Rct change. These results suggest that DNA and consequently negative charge near the electrode possess a larger impact on the impedimetric signal than DNA further away. Y1 - 2014 U6 - https://doi.org/10.1021/ac501800q SN - 1520-6882 (E-Journal); 0003-2700 (Print); 0096-4484 (Print) VL - 86 (2014) IS - 15 SP - 7867 EP - 7874 PB - ACS Publications CY - Columbus ER - TY - CHAP A1 - Schirra, Julian A1 - J., Watmuff T1 - Euler-based induced drag estimation for highly non-planar lifting systems during conceptional design T2 - 62. Deutscher Luft- und Raumfahrtkongress 2013 : 10. - 12. September 2032, Stuttgart, Haus der Wirtschaft Y1 - 2013 SP - 1 EP - 8 PB - Dt. Gesellschaft für Luft- und Raumfahrt CY - Bonn ER - TY - RPRT A1 - Hoffschmidt, Bernhard T1 - HelioScan : Machbarkeitsstudie zur Entwicklung einer radargestützten Positionsregelung von Heliostatenfeldern für Solarturmkraftwerke : Schlussbericht : Laufzeit: 01.07.2010 - 31.12.2012 : Förderkennzeichen 325234A Y1 - 2013 PB - Bundesministerium für Umwelt, Naturschutz und Reaktorsicherheit CY - Berlin ER - TY - JOUR A1 - Gebhardt, Andreas A1 - Fateri, Miranda T1 - 3D-Drucken und die Anwendungen JF - RTejournal - Forum für Rapid Technologie Y1 - 2014 SN - 1614-0923 VL - 11 (2014) IS - 1 SP - 1 EP - 9 ER - TY - JOUR A1 - Gebhardt, Andreas A1 - Hötter, Jan-Steffen A1 - Ziebura, Dawid T1 - Impact of SLM build parameters on the surface quality JF - RTejournal - Forum für Rapid Technologien Y1 - 2014 SN - 1614-0923 VL - 11 (2014) IS - 1 SP - 1 EP - 14 ER - TY - JOUR A1 - Martinez, Ronny A1 - Jakob, Felix A1 - Tu, Ran A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schwaneberg, Ulrich T1 - Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution JF - Biotechnology and bioengineering Y1 - 2013 SN - 1097-0290 (E-Journal); 0006-3592 (Print); 0368-1467 (Print) VL - Vol. 110 IS - Iss. 3 SP - 711 EP - 720 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Jakob, Felix A1 - Martinez, Ronny A1 - Mandawe, John A1 - Hellmuth, Hendrik A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schwaneberg, Ulrich T1 - Surface charge engineering of a Bacillus gibsonii subtilisin protease JF - Applied microbiology and biotechnology Y1 - 2013 SN - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print) VL - Vol. 97 IS - Iss. 15 SP - 6793 EP - 6802 PB - Springer CY - Berlin ER - TY - JOUR A1 - Niehaus, F. A1 - Gabor, E. A1 - Wieland, S. A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Eck, J. T1 - Enzymes for the laundry industries: tapping the vast metagenomic pool of alkaline proteases JF - Microbial biotechnology Y1 - 2011 SN - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print) VL - Vol. 4 IS - Iss. 6 SP - 767 EP - 776 PB - Springer CY - Berlin ER - TY - JOUR A1 - Ribitsch, D. A1 - Heumann, S. A1 - Karl, W. A1 - Gerlach, J. A1 - Leber, R. A1 - Birner-Gruenberger, R. A1 - Gruber, K. A1 - Eiteljoerg, I. A1 - Remler, P. A1 - Siegert, Petra A1 - Lange, J. A1 - Maurer, Karl-Heinz A1 - Berg, G. A1 - Guebitz, G. M. A1 - Schwab, H. T1 - Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli JF - Journal of biotechnology N2 - A large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. Several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. Encapsulation of cells in alginate beads induced protease production. Stenotrophomonas maltophilia emerged as best performer under washing conditions. For identification of wash-active proteases, four extracellular serine proteases called StmPr1, StmPr2, StmPr3 and StmPr4 were cloned. StmPr2 and StmPr4 were sufficiently overexpressed in E. coli. Expression of StmPr1 and StmPr3 resulted in unprocessed, insoluble protein. Truncation of most of the C-terminal domain which has been identified by enzyme modeling succeeded in expression of soluble, active StmPr1 but failed in case of StmPr3. From laundry application tests StmPr2 turned out to be a highly wash-active protease at 45 °C. Specific activity of StmPr2 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 17 ± 2 U/mg. In addition we determined the kinetic parameters and cleavage preferences of protease StmPr2. KW - Alginate beads KW - Stenotrophomonas maltophilia KW - Detergent protease Y1 - 2012 U6 - https://doi.org/10.1016/j.jbiotec.2011.09.025 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 157 IS - 1 SP - 140 EP - 147 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Schmitz, Philipp A1 - Weber, Martin T1 - Buying and selling behavior of individual investors in option-like securities JF - Die Betriebswirtschaft : DBW N2 - We analyze the trading behavior of individual investors in option-like securities, namely bankissued warrants, and thus expand the growing literature of investors behavior to a new kind of securities. A unique data set from a large German discount broker gives us the opportunity to analyze the trading behavior of 1,454 investors, making 89,958 transactions in 6,724 warrants on 397 underlyings. In different logit regression, we make use of the facts that investors can speculate on rising and falling prices of the underlying with call and put warrants and that we also have information about the stock portfolios of the investors. We report several facts about the trading behavior of individual investors in warrants that are consistent with the literature on the behavior of individual investors in the stock market. The warrant investors buy calls and sell puts if the price of the underlying has decreased over the past trading days and they sell calls and buy puts if the price of the underlying has increased. That means, the investors follow negative feedback trading strategies in all four trading categories observed. In addition, we find strong evidence for the disposition effect for call as well as put warrants, which is reversed in December. The trading behavior is also influenced if the underlying reaches some exceptionally prices, e.g. highs, lows or the strike price. We show that hedging, as one natural candidate to buy puts, does not play an important role in the market for bank-issued warrants. KW - Bank-issued Warrants KW - Disposition Effect KW - Negative Feedback Trading KW - Trading Behavior KW - Individual Investors Y1 - 2012 U6 - https://doi.org/10.2139/ssrn.2055088 SN - 0342-7064 VL - 72 IS - 5 SP - 409 EP - 426 PB - Schäffer-Poeschel CY - Stuttgart ER - TY - JOUR A1 - Schmitz, Philipp A1 - Weber, Martin T1 - Buying and selling behavior of individual investors in option-like securities Y1 - 2007 SP - 1 EP - 36 ER - TY - JOUR A1 - Glaser, Markus A1 - Schmitz, Philipp T1 - Privatanleger am Optionsscheinmarkt JF - Zeitschrift für Bankrecht und Bankwirtschaft : ZBB Y1 - 2007 SN - 0936-2800 N1 - Printausg. in der Bibliothek Eupener Str. vorhanden VL - Jg. 19 IS - H. 3 SP - 214 EP - 230 ER - TY - JOUR A1 - Glaser, Markus A1 - Schmitz, Philipp T1 - Privatanleger am Optionsscheinmarkt Y1 - 2006 SP - 1 EP - 46 ER - TY - CHAP A1 - Schmitz, Philipp A1 - Weber, Martin T1 - Setze nicht alles auf eine Karte, diversifiziere! T2 - Genial einfach investieren Y1 - 2007 SP - 104 EP - 140 PB - Campus-Verlag CY - Frankfurt a.M. ER - TY - THES A1 - Schmitz, Philipp T1 - Empirical analyses of the trading behavior of individual investors in the warrant market Y1 - 2007 N1 - Mannheim, Univ., Diss., 2007 ER - TY - PAT A1 - Siegert, Petra A1 - Merkel, Marion A1 - Kluin, Cornelia A1 - O'Connell, Timothy A1 - Maurer, Karl-Heinz T1 - Wasch- und Reinigungsmittel enthaltend Proteasen aus Xanthomonas [Offenlegungsschrift] T1 - Detergents and cleaners comprising proteases from xanthomonas [Internationale Patentanmeldung] Y1 - 2008 SP - 1 EP - 15 PB - Deutsches Patent- und Markenamt / WIPO CY - München / Genf ER - TY - CHAP A1 - Reichert, Walter T1 - Der Vertriebsingenieur: Ausbildung und Beruf T2 - Berufsstart Technik : das Karriereportal für junge Akademiker ; Ingenieure, Naturwissenschaftler, Informatiker Y1 - 2014 SP - 456 EP - 458 PB - Resch CY - Großenkneten ER - TY - JOUR A1 - Meiners, Klaus A1 - Baumann, Marcus T1 - Lokale Beiträge zum globalen Klimaschutz JF - Wechselwirkung : Wissenschaft & vernetztes Denken Y1 - 1997 SN - 0172-1623 VL - 19 IS - 84 SP - 6 EP - 12 ER - TY - JOUR A1 - Baumann, Marcus A1 - Gronsfeld, A. A1 - Schütz-Lembach, G. T1 - Nachdenklichkeit über Nachhaltigkeit : wie uns ein neues Wort alte Probleme neu entdecken läßt JF - Wechselwirkung : Wissenschaft & vernetztes Denken Y1 - 1997 SN - 0172-1623 VL - 19 IS - 87 SP - 6 EP - 11 ER - TY - JOUR A1 - Baumann, Marcus A1 - Peschel, G. T1 - Aktuelle Entwicklungen zur Luftreinhaltung in der Stadt Aachen JF - Wechselwirkung : Wissenschaft & vernetztes Denken Y1 - 1998 SN - 0172-1623 VL - 20 IS - 91 SP - 22 EP - 29 ER - TY - JOUR A1 - Baumann, Marcus A1 - Peschel, G. T1 - Das Klima Aachens, lokale Ansprüche und globale Auswirkungen JF - Wechselwirkung : Wissenschaft & vernetztes Denken Y1 - 1998 SN - 0172-1623 VL - 20 IS - 93 SP - 26 EP - 33 ER - TY - JOUR A1 - Wiezorek, E. A1 - Baumann, Marcus T1 - Stadtökologie in der Stadtplanung: der "Stadtökologische Beitrag" Aachen JF - Wechselwirkung : Wissenschaft & vernetztes Denken Y1 - 1999 SN - 0172-1623 VL - 21 IS - 97 SP - 28 EP - 37 ER - TY - RPRT A1 - Schöning, Michael Josef A1 - Selmer, Thorsten A1 - Baumann, Marcus T1 - Schlussbericht zum Projekt "Bio-LAPS" : Optimierung des Betriebs eines Biogasfermenters mit Hilfe eines Feldeffekt-Biosensors auf Basis eines lichtadressierbaren potentiometrischen Sensors (LAPS) : Laufzeit: 01.09.2008 bis 31.01.2012 : Förderkennzeichen 07NR264 bzw.22026407 Y1 - 2012 PB - BMELV CY - Berlin ER - TY - JOUR A1 - Ribitsch, D. A1 - Heumann, S. A1 - Trotscha, E. A1 - Herrero Acero, E. A1 - Greimel, K. A1 - Leber, R. A1 - Birger-Gruenberger, R. A1 - Deller, S. A1 - Eiteljoerg, I. A1 - Remler, P. A1 - Weber, Th. A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Donelli, I. A1 - Freddi, G. A1 - Schwab, H. A1 - Guebitz, G. M. T1 - Hydrolysis of polyethyleneterephthalate by p-nitrobenzylesterase from Bacillus subtilis JF - Biotechnology progress Y1 - 2011 SN - 1520-6033 (E-Journal); 8756-7938 (Print) VL - Vol. 27 IS - Iss. 4 SP - 951 EP - 960 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Ribitsch, D. A1 - Karl, W. A1 - Birner-Gruenberger, R. A1 - Gruber, K. A1 - Eiteljoerg, I. A1 - Remler, P. A1 - Wieland, S. A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schwab, H. T1 - C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli JF - Journal of biotechnology N2 - Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7–11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease. Y1 - 2010 U6 - https://doi.org/10.1016/j.jbiotec.2010.09.947 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 150 IS - 3 SP - 408 EP - 416 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Wendorff, Marion A1 - Eggert, Thorsten A1 - Pohl, Martina A1 - Dresen, Carola A1 - Müller, Michael A1 - Jaeger, Karl-Erich A1 - Sprenger, Georg A. A1 - Schürmann, Melanie A1 - Schürmann, Martin A1 - Johnen, Sandra A1 - Sprenger, Gerda A1 - Sahm, Hermann A1 - Inoue, Tomoyuki A1 - Schörken, Ulrich A1 - Breittaupt, Holger A1 - Frölich, Bettina A1 - Heim, Petra A1 - Iding, Hans A1 - Juchem, Bettina A1 - Siegert, Petra A1 - Kula, Maria-Regina A1 - Weckbecker, Andrea A1 - Hummel, Werner A1 - Fessner, Wolf-Dieter A1 - Elling, Lothar A1 - Wolberg, Michael A1 - Bode, Silke A1 - Feldmann, Ralf A1 - Geilenkirchen, Petra A1 - Schubert, Thomas A1 - Walter, Lydia A1 - Dünnwald, Thomas A1 - Demir, Ayhan S. A1 - Kolter-Jung, Doris A1 - Nitsche, Adam A1 - Dünkelmann, Pascal A1 - Cosp, Annabel A1 - Lingen, Bettina T1 - Catalytic asymmetric synthesis : section 2.2 T2 - Asymmetric synthesis with chemical and biological methods / ed. by Dieter Enders ... Y1 - 2007 SN - 978-3-527-31473-7 SP - 298 EP - 413 PB - Wiley-VCH CY - Weinheim ER -