TY  - JOUR
A1  - Digel, Ilya
A1  - Wehlitz, V.
A1  - Kayser, Peter
A1  - Figiel-Lange, A.
A1  - Bassam, Rasha
A1  - Rundstedt, F. von
T1  - Suspension depletion approach for exemption of infected Solanum jasminoides cells from pospiviroids
JF  - Plant Pathology
N2  - Despite numerous studies, viroid elimination from infected plants remains a very challenging task. This study introduces for the first time a novel ‘suspension depletion’ approach for exemption of Solanum jasminoides plants from viroids. The proposed method implies initial establishment of suspension cultures of the infected plant cells. The suspended cells were then physically treated (mild thermotherapy, 33 °C), which presumably delayed the replication of the viroid. The viroid concentration in the treated biomass was monitored weekly using pospiviroid-specific PCR. After 10–12 weeks of continuous treatment, a sufficient decrease in viroid concentration was observed such that the infection became undetectable by PCR. The treated single cells then gave rise to microcolonies on a solid culture medium and the obtained viroid-negative clones were further promoted to regenerate into viroid-free plants. Three years of accumulated experimental data suggests feasibility, broad applicability, and good efficacy of the proposed approach.
Y1  - 2018
U6  - https://doi.org/10.1111/ppa.12750
SN  - 1365-3059
VL  - 67
IS  - 2
SP  - 358
EP  - 365
PB  - Wiley
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Staat, Manfred
A1  - Baroud, Gamal
A1  - Topcu, M.
A1  - Sponagel, Stefan
T1  - Soft Materials in Technology and Biology – Characteristics, Properties, and Parameter Identification
JF  - Bioengineering in Cell and Tissue Research / Artmann, Gerhard M. ; Chien, Shu (Eds.)
Y1  - 2008
SN  - 978-3-540-75408-4
SP  - 253
EP  - 315
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Yalcin, Ozlem
A1  - Resmi, Halil
A1  - Başkurt, Oǧuz Kerim
T1  - Can white blood cell activation be one of the major factors that affect hemorheological parameters during and after exercise?
JF  - Clinical Hemorheology and Microcirculation. 26 (2002), H. 3
Y1  - 2002
SN  - 1386-0291
N1  - = The 11th European Symposium on Clinical Hemorheology, Rouen, France, 19-22 September 2000
SP  - 189
EP  - 193
ER  - 
TY  - JOUR
A1  - Sponagel, Stefan
A1  - Baroud, Gamal
A1  - Falk, R.
A1  - Crookshank, M.
T1  - Corrigendum to “Experimental and theoretical investigation of directional permeability of human vertebral cancellous bone for cement infiltration”. Baroud, G.; Falk, R.; Crookshank, M.; Sponagel, S.; Steffen, T.
JF  - Journal of Biomechanics. 37 (2004), H. 2
Y1  - 2004
SN  - 0021-9290
SP  - 1802
ER  - 
TY  - JOUR
A1  - Sponagel, Stefan
A1  - Baroud, Gamal
A1  - Steffen, T.
A1  - Wu, J.
T1  - Parameter identification for cement infiltration of osteoporotic bone
JF  - Poromechanics II : Proceedings of the Second Biot Conference on Poromechanics Grenoble, France 26 - 28 August 2002 / ed. by J.-L. Auriault ...
Y1  - 2002
SN  - 90-5809-394-8
SP  - 55
EP  - 58
PB  - Balkema
CY  - Lisse [u.a.]
ER  -