TY  - JOUR
A1  - Elbers, Gereon
A1  - Pfeffer, H.-U.
A1  - Friesel, J.
A1  - Beier, Reinhold
T1  - Pilotuntersuchungen gemäß dem Verordnungsentwurf nach § 40, Abs. 2 des Bundesimmissionsschutzgesetzes / H.-U. Pfeffer, J. Friesel, G. Elbers, R. Beier, K. Ellermann
JF  - Drei Jahrzehnte Luftqualitätsüberwachung : Vorträge und Berichte zum Kolloquium "Drei Jahrzehnte Luftqualitätsüberwachung - Bilanz und Perspektiven" am 11. November 1993 in Essen / [Hrsg.: Landesanstalt für Immissionsschutz Nordrhein-Westfalen. Red. Bearb.: Robert Niesenhaus]
Y1  - 1993
N1  - LIS-Berichte ; 110 ;
SP  - 117
CY  - Essen
ER  - 
TY  - JOUR
A1  - Ribitsch, Doris
A1  - Karl, Wolfgang
A1  - Birner-Gruenberger, Ruth
A1  - Gruber, Karl
A1  - Eiteljoerg, Inge
A1  - Remler, Peter
A1  - Wieland, Susanne
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
A1  - Schwab, Helmut
T1  - C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli
JF  - Journal of biotechnology
N2  - Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477.

This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7–11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.
Y1  - 2010
U6  - https://doi.org/10.1016/j.jbiotec.2010.09.947
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - 150
IS  - 3
SP  - 408
EP  - 416
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Adels, Klaudia
A1  - Thönnessen, Vera
A1  - Monakhova, Yulia
T1  - Complementary instrumental techniques applied to pain relieving tablets in an undergraduate laboratory experiment
JF  - Journal of Chemical Education
N2  - Several unconnected laboratory experiments are usually offered for students in instrumental analysis lab. To give the students a more rational overview of the most common instrumental techniques, a new laboratory experiment was developed. Marketed pain relief drugs, familiar consumer products with one to three active components, namely, acetaminophen (paracetamol), acetylsalicylic acid (ASA), and caffeine, were selected. Common analytical methods were compared regarding the performance of qualitative and quantitative analysis of unknown tablets: UV–visible (UV–vis), infrared (IR), and nuclear magnetic resonance (NMR) spectroscopies, as well as high-performance liquid chromatography (HPLC). The students successfully uncovered the composition of formulations, which were divided into three difficulty categories. Students were shown that in addition to simple mixtures handled in theoretical classes, the composition of complex drug products can also be uncovered. By comparing the performance of different techniques, students deepen their understanding and compare the efficiency of analytical methods in the context of complex mixtures. The laboratory experiment can be adjusted for graduate level by including extra tasks such as method optimization, validation, and 2D spectroscopic techniques.
KW  - Instrumental analysis
KW  - Spectroscopy
KW  - Liquid chromatography
KW  - Qualitative and quantitative determination
KW  - Drugs
Y1  - 2024
U6  - https://doi.org/10.1021/acs.jchemed.4c00681
SN  - 0021-9584 (Print)
SN  - 1938-1328 (Online)
PB  - ACS
ER  - 
TY  - JOUR
A1  - Raue, Markus
A1  - Wambach, M.
A1  - Glöggler, Stefan
A1  - Grefen, Dana
A1  - Kaufmann, R.
A1  - Abetz, C.
A1  - Georgopanos, Prokopios
A1  - Handge, U. A.
A1  - Mang, Thomas
A1  - Blümich, Bernhard
A1  - Abetz, V.
T1  - Investigation of historical hard rubber ornaments of Charles Goodyear
JF  - Macromolecular chemistry and physics
Y1  - 2014
SN  - 1022-1352
VL  - Vol. 215
IS  - No. 3
SP  - 245
EP  - 254
PB  - Wiley-VCH
CY  - Weinheim
ER  -