TY  - JOUR
A1  - Wu, Chunsheng
A1  - Bronder, Thomas
A1  - Poghossian, Arshak
A1  - Werner, Frederik
A1  - Schöning, Michael Josef
T1  - Label-free detection of DNA using light-addressable potentiometric sensor modified with a positively charged polyelectrolyte layer
JF  - Nanoscale
N2  - A multi-spot (16 spots) light-addressable potentiometric sensor (MLAPS) consisting of an Al–p-Si–SiO2 structure modified with a weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)) was applied for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge for the first time. To achieve a preferentially flat orientation of DNA strands and thus, to reduce the distance between the DNA charge and MLAPS surface, the negatively charged probe single-stranded DNAs (ssDNA) were electrostatically adsorbed onto the positively charged PAH layer using a simple layer-by-layer (LbL) technique. In this way, more DNA charge can be positioned within the Debye length, yielding a higher sensor signal. The surface potential changes in each spot induced due to the surface modification steps (PAH adsorption, probe ssDNA immobilization, hybridization with complementary target DNA (cDNA), non-specific adsorption of mismatched ssDNA) were determined from the shifts of photocurrent–voltage curves along the voltage axis. A high sensor signal of 83 mV was registered after immobilization of probe ssDNA onto the PAH layer. The hybridization signal increases from 5 mV to 32 mV with increasing the concentration of cDNA from 0.1 nM to 5 μM. In contrast, a small signal of 5 mV was recorded in the case of non-specific adsorption of fully mismatched ssDNA (5 μM). The obtained results demonstrate the potential of the MLAPS in combination with the simple and rapid LbL immobilization technique as a promising platform for the future development of multi-spot light-addressable label-free DNA chips with direct electrical readout.
Y1  - 2015
U6  - http://dx.doi.org/10.1039/C4NR07225A
VL  - 14
IS  - 7
SP  - 6143
EP  - 6150
PB  - Royal Society of Chemistry (RSC)
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Bronder, Thomas
A1  - Wu, Chunsheng
A1  - Poghossian, Arshak
A1  - Werner, Frederik
A1  - Keusgen, M.
A1  - Schöning, Michael Josef
T1  - Label-free detection of DNA hybridization with light-addressable potentiometric sensors: comparison of various DNA-immobilization strategies
JF  - Procedia Engineering
N2  - Light-addressable potentiometric sensors (LAPS) consisting of a p-Si-SiO2 and p-Si-SiO2-Au structure, respectively, have been tested for a label-free electrical detection of DNA (deoxyribonucleic acid) hybridization. Three different strategies for immobilizing single-stranded probe DNA (ssDNA) molecules on a LAPS surface have been studied and compared: (a) immobilization of thiol-modified ssDNA on the patterned Au surface via gold-thiol bond, (b) covalent immobilization of amino-modified ssDNA onto the SiO2 surface functionalized with 3-aminopropyltriethoxysilane and (c) layer-by-layer adsorption of negatively charged ssDNA on a positively charged weak polyelectrolyte layer of poly(allylamine hydrochloride).
KW  - LAPS
KW  - lable-free detection
KW  - DNA hybridization
KW  - field-effect sensor
Y1  - 2014
U6  - http://dx.doi.org/10.1016/j.proeng.2014.11.647
SN  - 1877-7058
N1  - EUROSENSORS 2014 ; European Conference on Solid-State Transducers <28, 2014>
VL  - 87
SP  - 755
EP  - 758
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Ingebrandt, S.
A1  - Abouzar, Maryam H.
A1  - Schöning, Michael Josef
T1  - Label-free detection of charged macromolecules by using a field-effect-based sensor platform: Experiments and possible mechanisms of signal generation
JF  - Applied Physics A: Materials Science & Processing. 87 (2007), H. 3
Y1  - 2007
SN  - 0947-8396
N1  - Special Issue “From Surface Science to Nanoscale Devices”
SP  - 517
EP  - 524
ER  - 
TY  - JOUR
A1  - Mourzina, Y.
A1  - Mai, T.
A1  - Poghossian, Arshak
A1  - Ermolenko, Y.
A1  - Yoshinobu, T.
A1  - Vlasov, Y.
A1  - Iwasaki, H.
A1  - Schöning, Michael Josef
T1  - K+-selective field-effect sensors as transducers for bioelectronic applications
JF  - Electrochimica Acta. 48 (2003), H. 20-22
Y1  - 2003
SN  - 0013-4686
SP  - 3333
EP  - 3339
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Sauke, M.
A1  - Steffen, A.
A1  - Marso, M. (u.a.)
T1  - Ion-sensitive field-effect transistors with ultrathin Langmuir-Blodgett membranes
JF  - Sensors and Actuators B. 27 (1995), H. 1-3
Y1  - 1995
SN  - 0925-4005
SP  - 325
EP  - 328
ER  - 
TY  - JOUR
A1  - Mourzina, Y. G.
A1  - Yoshinobu, T.
A1  - Schubert, J.
A1  - Lüth, H.
A1  - Iwasaki, H.
A1  - Schöning, Michael Josef
T1  - Ion-selective lightaddressable potentiometric sensor (LAPS) with chalcogenide thin film by pulsed laser deposition
JF  - Sensors and Actuators B. 80 (2001), H. 2
Y1  - 2001
SN  - 0925-4005
SP  - 136
EP  - 140
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Bruns, M.
A1  - Hoffmann, W.
A1  - Hoffmann, B. (u.a.)
T1  - Iodide ion-sensitive field-effect structures
JF  - Sensors and Actuators B. 15 (1993), H. 1-3
Y1  - 1993
SN  - 0925-4005
SP  - 192
EP  - 194
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Crott, M.
A1  - Ronkel, F.
A1  - Thust, M.
A1  - Schultze, J. W.
A1  - Kordos, P.
A1  - Lüth, H.
T1  - Investigations on porous silicon layers with regard to chemical microsensor applications
JF  - Third International Conference on Intelligent Materials : Lyon, 3-4-5 June 1996, Center of Congress "L'Espace Tête d'Or" / [publ. by SPIE, the International Society for Optical Engineering]. General chair/ed. by: P. F. Gobin
Y1  - 1996
SN  - 0819421650
N1  - International Conference on Intelligent Materials ; (3, 1996, Lyon)
SP  - 275
EP  - 280
PB  - SPIE
CY  - Bellingham, Wash.
ER  - 
TY  - JOUR
A1  - Breuer, Lars
A1  - Mang, Thomas
A1  - Schöning, Michael Josef
A1  - Thoelen, Ronald
A1  - Wagner, Torsten
T1  - Investigation of the spatial resolution of a laser-based stimulation process for light-addressable hydrogels with incorporated graphene oxide by means of IR thermography
JF  - Sensors and Actuators A: Physical
Y1  - 2017
U6  - http://dx.doi.org/10.1016/j.sna.2017.11.031
SN  - 0924-4247
VL  - 268
SP  - 126
EP  - 132
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Ismail, A.B.M.
A1  - Harada, T.
A1  - Yoshinobu, T.
A1  - Iwasaki, H.
A1  - Schöning, Michael Josef
A1  - Lüth, H.
T1  - Investigation of pulsed laser-deposited Al2O3 as a high pH-sensitive layer for LAPS-based biosensing applications
JF  - Sensors and Actuators B. 71 (2000), H. 3
Y1  - 2000
SN  - 0925-4005
SP  - 169
EP  - 172
ER  - 
TY  - JOUR
A1  - Näther, Niko
A1  - Henkel, Hartmut
A1  - Schneider, Andreas
A1  - Schöning, Michael Josef
T1  - Investigation of different catalytically active and passive materials for realising a hydrogen peroxide gas sensor
JF  - physica status solidi (a) . 206 (2009), H. 3
Y1  - 2009
SN  - 1862-6319
N1  - Special Issue: Engineering of Functional Interfaces (EnFI 08)
SP  - 449
EP  - 454
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Krämer, Melina
A1  - Abouzar, Maryam H.
A1  - Pita, Marcos
A1  - Katz, Evgeny
A1  - Schöning, Michael Josef
T1  - Interfacing of biocomputing systems with silicon chips: Enzyme logic gates based on field-effect devices
JF  - Procedia Chemistry. 1 (2009), H. 1
Y1  - 2009
SN  - 1876-6196
N1  - Proceedings of the Eurosensors XXIII conference ; Eurosensors 23
SP  - 682
EP  - 685
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Malzahn, K.
A1  - Abouzar, Maryam H.
A1  - Mehndiratta, P.
A1  - Katz, E.
A1  - Schöning, Michael Josef
T1  - Integration of biomolecular logic gates with field-effect transducers
JF  - Electrochimica Acta. 56 (2011), H. 26
Y1  - 2011
SN  - 0013-4686
SP  - 9661
EP  - 9665
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Rolka, David
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
T1  - Integration of a capacitive EIS sensor into a FIA system for pH and penicillin determination
JF  - Sensors. 4 (2004)
Y1  - 2004
SN  - 1424-8220
SP  - 84
EP  - 94
ER  - 
TY  - JOUR
A1  - Schroth, P.
A1  - Schöning, Michael Josef
A1  - Kordos, P.
A1  - Lüth, H.
A1  - Schütz, S.
A1  - Weißbecker, B.
A1  - Hummel, Hans E.
T1  - Insectbased BioFETs with improved signal characteristics
JF  - Biosensors & Bioelectronics. 14 (1999), H. 3
Y1  - 1999
SN  - 0956-5663
SP  - 303
EP  - 308
ER  - 
TY  - JOUR
A1  - Schütz, S.
A1  - Czynski, J.
A1  - Hummel, Hans E.
A1  - Schöning, Michael Josef
A1  - Schroth, P.
A1  - Kordos, P.
A1  - Lüth, H.
T1  - Insect meets microelectronics: A BioFET as a first step to bioelectronic microsystems
JF  - Transducers '99 : proceedings of the Tenth International Conference on Solid-State Sensors and Actuators ; 7 - 10 June, 1999, Sendai, Japan / ed.: S. Middelhoek
Y1  - 2000
N1  - International Conference on Solid State Sensors and Actuators <10, 1999, Sendai, Miyagi-ken> ; Transducers '99 ; (10 : ; 1999.06.07-10 : ; Sendai)
SP  - 1104
EP  - 1107
PB  - Elsevier
CY  - Amsterdam [u.a.]
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Schroth, Peter
A1  - Lüth, Hans
A1  - Hummel, Hans E.
A1  - Schütz, Stefan
T1  - Insect chemoreceptors coupled to silicon transistors as innovative biosensors
JF  - Proceedings of SPIE. 4205 (2001)
Y1  - 2001
SP  - 152
EP  - 162
ER  - 
TY  - JOUR
A1  - Kloock, Joachim P.
A1  - Mourzina, Y.G.
A1  - Ermolenko, Y.
A1  - Doll, T.
A1  - Schubert, J.
A1  - Schöning, Michael Josef
T1  - Inorganic thin-film sensor membranes with PLD-prepared chalcogenide glasses: Challenges and implementation
JF  - Sensors. 4 (2004), H. 10
Y1  - 2004
SN  - 1424-8220
SP  - 156
EP  - 162
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Schubert, J
A1  - Kloock, Joachim P.
A1  - Zander, W.
A1  - Mourzina, Y. G.
A1  - Legin, A.
A1  - Vlasov, Y. G.
A1  - Lüth, H.
T1  - Innovative thin film techniques for microfabricating electrochemical sensors
JF  - Lecture Notes of the ICB Seminars
Y1  - 2002
SP  - 55
EP  - 66
PB  - MCB
CY  - Warsaw
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Schubert, Joachim P.
A1  - Kloock, Joachim P.
A1  - Zander, W.
A1  - Mourzina, Y. G.
A1  - Legin, A.
A1  - Vlasov, Y. G.
A1  - Lüth, H.
T1  - Innovative thin film techniques for microfabricating electrochemical sensors
JF  - Biocybernetics and Biomedical Engineering. 21 (2001), H. 4
Y1  - 2001
SN  - 0208-5216
SP  - 107
EP  - 119
ER  -