TY - JOUR A1 - Kappler-Tanudyaya, Nathalie A1 - Schmitt, Heike A1 - Tippkötter, Nils A1 - Meyer, Lina A1 - Lenzen, Sigurd A1 - Ulber, Roland T1 - Combination of biotransformation and chromatography for the isolation and purification of mannoheptulose JF - Biotechnology Journal N2 - Mannoheptulose is a seven-carbon sugar. It is an inhibitor of glucose-induced insulin secretion due to its ability to selectively inhibit the enzyme glucokinase. An improved procedure for mannoheptulose isolation from avocados is described in this study (based upon the original method by La Forge). The study focuses on the combination of biotransformation and downstream processing (preparative chromatography) as an efficient method to produce a pure extract of mannoheptulose. The experiments were divided into two major phases. In the first phase, several methods and parameters were compared to optimize the mannoheptulose extraction with respect to efficiency and purity. In the second phase, a mass balance of mannoheptulose over the whole extraction process was undertaken to estimate the yield and efficiency of the total extraction process. The combination of biotransformation and preparative chromatography allowed the production of a pure mannoheptulose extract. In a biological test, the sugar inhibited the glucokinase enzyme activity efficiently. Y1 - 2007 U6 - https://doi.org/10.1002/biot.200700004 SN - 1860-7314 VL - 2 IS - 6 SP - 692 EP - 699 ER - TY - JOUR A1 - Götten, Falk A1 - Havermann, Marc A1 - Braun, Carsten A1 - Marino, Matthew A1 - Bil, Cees T1 - Aerodynamic Investigations of UAV Sensor Turrets - A Combined Wind-tunnel and CFD Approach JF - SciTech 2021, AIAA SciTech Forum, online, WW, Jan 11-15, 2021 Y1 - 2021 U6 - https://doi.org/10.2514/6.2021-1535 SP - 1 EP - 12 PB - AIAA CY - Reston, Va. ER - TY - JOUR A1 - Göttsche, Joachim A1 - Reilly, S. A1 - Wittwer, Volker T1 - Advanced window systems and building energy performance / S. Reilly ; J. Göttsche ; V. Wittwer JF - Solar World Congress, 1991 : proceedings of the biennial congress of the International Solar Energy Society, Denver, Colorado, USA, 19-23 August 1991 / ed. by M. E. Arden ... Y1 - 1991 SN - 0-08-041690-X SP - 3211 EP - 3216 PB - Pergamon Press CY - Oxford [u.a.] ER - TY - JOUR A1 - Kurulgan Demirci, Eylem A1 - Demirci, Taylan A1 - Linder, Peter A1 - Trzewik, Jürgen A1 - Gierkowski, Jessica Ricarda A1 - Gossmann, Matthias A1 - Kayser, Peter A1 - Porst, Dariusz A1 - Digel, Ilya A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül T1 - rhAPC reduces the endothelial cell permeability via a decrease of contractile tensions induced by endothelial cells JF - Journal of Bioscience and Bioengineering N2 - All cells generate contractile tension. This strain is crucial for mechanically controlling the cell shape, function and survival. In this study, the CellDrum technology quantifying cell's (the cellular) mechanical tension on a pico-scale was used to investigate the effect of lipopolysaccharide (LPS) on human aortic endothelial cell (HAoEC) tension. The LPS effect during gram-negative sepsis on endothelial cells is cell contraction causing endothelium permeability increase. The aim was to finding out whether recombinant activated protein C (rhAPC) would reverse the endothelial cell response in an in-vitro sepsis model. In this study, the established in-vitro sepsis model was confirmed by interleukin 6 (IL-6) levels at the proteomic and genomic levels by ELISA, real time-PCR and reactive oxygen species (ROS) activation by florescence staining. The thrombin cellular contraction effect on endothelial cells was used as a positive control when the CellDrum technology was applied. Additionally, the Ras homolog gene family, member A (RhoA) mRNA expression level was checked by real time-PCR to support contractile tension results. According to contractile tension results, the mechanical predominance of actin stress fibers was a reason of the increased endothelial contractile tension leading to enhanced endothelium contractility and thus permeability enhancement. The originality of this data supports firstly the basic measurement principles of the CellDrum technology and secondly that rhAPC has a beneficial effect on sepsis influenced cellular tension. The technology presented here is promising for future high-throughput cellular tension analysis that will help identify pathological contractile tension responses of cells and prove further cell in-vitro models. KW - Cell permeability KW - Cellular force KW - Endothelial cells KW - Recombinant activated protein C KW - Lipopolysaccharide KW - Contractile tension KW - CellDrum Y1 - 2012 U6 - https://doi.org/10.1016/j.jbiosc.2012.03.019 SN - 1347-4421 VL - 113 IS - 2 SP - 212 EP - 219 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Ringers, Christa A1 - Bialonski, Stephan A1 - Ege, Mert A1 - Solovev, Anton A1 - Hansen, Jan Niklas A1 - Jeong, Inyoung A1 - Friedrich, Benjamin M. A1 - Jurisch-Yaksi, Nathalie T1 - Novel analytical tools reveal that local synchronization of cilia coincides with tissue-scale metachronal waves in zebrafish multiciliated epithelia JF - eLife N2 - Motile cilia are hair-like cell extensions that beat periodically to generate fluid flow along various epithelial tissues within the body. In dense multiciliated carpets, cilia were shown to exhibit a remarkable coordination of their beat in the form of traveling metachronal waves, a phenomenon which supposedly enhances fluid transport. Yet, how cilia coordinate their regular beat in multiciliated epithelia to move fluids remains insufficiently understood, particularly due to lack of rigorous quantification. We combine experiments, novel analysis tools, and theory to address this knowledge gap. To investigate collective dynamics of cilia, we studied zebrafish multiciliated epithelia in the nose and the brain. We focused mainly on the zebrafish nose, due to its conserved properties with other ciliated tissues and its superior accessibility for non-invasive imaging. We revealed that cilia are synchronized only locally and that the size of local synchronization domains increases with the viscosity of the surrounding medium. Even though synchronization is local only, we observed global patterns of traveling metachronal waves across the zebrafish multiciliated epithelium. Intriguingly, these global wave direction patterns are conserved across individual fish, but different for left and right noses, unveiling a chiral asymmetry of metachronal coordination. To understand the implications of synchronization for fluid pumping, we used a computational model of a regular array of cilia. We found that local metachronal synchronization prevents steric collisions, i.e., cilia colliding with each other, and improves fluid pumping in dense cilia carpets, but hardly affects the direction of fluid flow. In conclusion, we show that local synchronization together with tissue-scale cilia alignment coincide and generate metachronal wave patterns in multiciliated epithelia, which enhance their physiological function of fluid pumping. Y1 - 2023 U6 - https://doi.org/10.7554/eLife.77701 SN - 2050-084X VL - 12 PB - eLife Sciences Publications ER - TY - JOUR A1 - Demirci, T. A1 - Trzewik, J. A1 - Linder, Peter A1 - Digel, Ilya A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül T1 - Mechanical Stimulation of 3T3 Fibroblasts Activates Genes: ITGB5 and p53 Responses as Quantified on the mRNA Level JF - Biomedizinische Technik . 49 (2004), H. Erg.-Bd. 2 Y1 - 2004 SN - 0932-4666 SP - 1030 EP - 1031 ER - TY - JOUR A1 - Asante-Asamani, E.O. A1 - Kleefeld, Andreas A1 - Wade, B.A. T1 - A second-order exponential time differencing scheme for non-linear reaction-diffusion systems with dimensional splitting JF - Journal of Computational Physics N2 - A second-order L-stable exponential time-differencing (ETD) method is developed by combining an ETD scheme with approximating the matrix exponentials by rational functions having real distinct poles (RDP), together with a dimensional splitting integrating factor technique. A variety of non-linear reaction-diffusion equations in two and three dimensions with either Dirichlet, Neumann, or periodic boundary conditions are solved with this scheme and shown to outperform a variety of other second-order implicit-explicit schemes. An additional performance boost is gained through further use of basic parallelization techniques. KW - Exponential time differencing KW - Real distinct pole KW - Dimensional splitting KW - Reaction-diffusion systems KW - Matrix exponential Y1 - 2020 U6 - https://doi.org/10.1016/j.jcp.2020.109490 SN - 0021-9991 N1 - Corresponding author: Andreas Kleefeld VL - 415 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Rausch, Valentin A1 - Harbrecht, Andreas A1 - Kahmann, Stephanie Lucina A1 - Fenten, Thomas A1 - Jovanovic, Nebojsa A1 - Hackl, Michael A1 - Müller, Lars P. A1 - Staat, Manfred A1 - Wegmann, Kilian T1 - Osteosynthesis of Phalangeal Fractures: Biomechanical Comparison of Kirschner Wires, Plates, and Compression Screws JF - The Journal of Hand Surgery N2 - Purpose The aim of this study was to compare several osteosynthesis techniques (intramedullary headless compression screws, T-plates, and Kirschner wires) for distal epiphyseal fractures of proximal phalanges in a human cadaveric model. Methods A total of 90 proximal phalanges from 30 specimens (index, ring, and middle fingers) were used for this study. After stripping off all soft tissue, a transverse distal epiphyseal fracture was simulated at the proximal phalanx. The 30 specimens were randomly assigned to 1 fixation technique (30 per technique), either a 3.0-mm intramedullary headless compression screw, locking plate fixation with a 2.0-mm T-plate, or 2 oblique 1.0-mm Kirschner wires. Displacement analysis (bending, distraction, and torsion) was performed using optical tracking of an applied random speckle pattern after osteosynthesis. Biomechanical testing was performed with increasing cyclic loading and with cyclic load to failure using a biaxial torsion-tension testing machine. Results Cannulated intramedullary compression screws showed significantly less displacement at the fracture site in torsional testing. Furthermore, screws were significantly more stable in bending testing. Kirschner wires were significantly less stable than plating or screw fixation in any cyclic load to failure test setup. Conclusions Intramedullary compression screws are a highly stable alternative in the treatment of transverse distal epiphyseal phalangeal fractures. Kirschner wires seem to be inferior regarding displacement properties and primary stability. Clinical relevance Fracture fixation of phalangeal fractures using plate osteosynthesis may have the advantage of a very rigid reduction, but disadvantages such as stiffness owing to the more invasive surgical approach and soft tissue irritation should be taken into account. Headless compression screws represent a minimally invasive choice for fixation with good biomechanical properties. Y1 - 2020 U6 - https://doi.org/10.1016/j.jhsa.2020.04.010 SN - 0363-5023 VL - 45 IS - 10 SP - 987.e1 EP - 987.e8 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Muschallik, Lukas A1 - Kipp, Carina Ronja A1 - Recker, Inga A1 - Bongaerts, Johannes A1 - Pohl, Martina A1 - Gelissen, Melanie A1 - Schöning, Michael Josef A1 - Selmer, Thorsten A1 - Siegert, Petra T1 - Synthesis of α-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13T butane-2, 3-diol dehydrogenase JF - Journal of Biotechnology N2 - The enantioselective synthesis of α-hydroxy ketones and vicinal diols is an intriguing field because of the broad applicability of these molecules. Although, butandiol dehydrogenases are known to play a key role in the production of 2,3-butandiol, their potential as biocatalysts is still not well studied. Here, we investigate the biocatalytic properties of the meso-butanediol dehydrogenase from Bacillus licheniformis DSM 13T (BlBDH). The encoding gene was cloned with an N-terminal StrepII-tag and recombinantly overexpressed in E. coli. BlBDH is highly active towards several non-physiological diketones and α-hydroxyketones with varying aliphatic chain lengths or even containing phenyl moieties. By adjusting the reaction parameters in biotransformations the formation of either the α-hydroxyketone intermediate or the diol can be controlled. Y1 - 2020 SN - 2590-1559 U6 - https://doi.org/10.1016/j.jbiotec.2020.09.016 VL - 202 IS - Vol. 324 SP - 61 EP - 70 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Hunker, Jan L. A1 - Gossmann, Matthias A1 - Raman, Aravind Hariharan A1 - Linder, Peter T1 - Artificial neural networks in cardiac safety assessment: Classification of chemotherapeutic compound effects on hiPSC-derived cardiomyocyte contractility JF - Journal of Pharmacological and Toxicological Methods Y1 - 2021 U6 - https://doi.org/10.1016/j.vascn.2021.107044 SN - 1056-8719 VL - 111 IS - Article number 107044 PB - Elsevier CY - New York ER - TY - JOUR A1 - Ferrein, Alexander A1 - Hermanns, Lutz A1 - Lakemeyer, Gerhard T1 - Comparing Sensor Fusion Techniques for Ball Position Estimation / Ferrein, Alexander ; Hermanns, Lutz ; Lakemeyer, Gerhard JF - RoboCup 2005: Robot Soccer World Cup IX Y1 - 2006 SN - 978-3-540-35437-6 N1 - Lecture Notes in Computer Science ; 4020 SP - 154 EP - 165 PB - Springer CY - Berlin ER - TY - JOUR A1 - Ferrein, Alexander A1 - Schiffer, Stefan A1 - Lakemeyer, Gerhard T1 - Football is coming Home / Schiffer, Stefan ; Ferrein, Alexander ; Lakemeyer, Gerhard JF - PCAR '06 Proceedings of the 2006 international symposium on Practical cognitive agents and robots Y1 - 2006 SN - 1-74052-130-7 SP - 39 EP - 50 PB - ACM CY - New York, NY ER - TY - JOUR A1 - Heuermann, Holger T1 - Multiport S-parameter calculation from two-port network analyzer measurements with or without switch matrix JF - ARFTG Conference, 2006 67th, San Francisco, CA Y1 - 2006 SN - 978-0-7803-9529-9 SP - 219 EP - 222 ER - TY - JOUR A1 - Heuermann, Holger A1 - Erkens, H. T1 - Novel RF switch concepts for differential wireless communications frontends. Erkens, H.; Heuermann, H. JF - IEEE Transactions on Microwave Theory and Techniques. 54 (2006), H. 6, Part 1 Y1 - 2006 SN - 0018-9480 SP - 2376 EP - 2382 ER - TY - JOUR A1 - Heuermann, Holger T1 - Verringerung des Phasenrauschens von Oszillatoren durch Dual-Mode-Resonatoren JF - HF-Report. 20 (2006), H. 5-6 Y1 - 2006 SN - 1431-827X SP - 43 EP - 48 ER - TY - JOUR A1 - Weigand, Christoph T1 - Economic Machine Adjustment in the Case of Product Screening .E.v.Collani, Ch. Weigand JF - Statistical Papers. 33 (1992) Y1 - 1992 SP - 171 EP - 184 ER - TY - JOUR A1 - Staat, Manfred A1 - Schwartz, M. A1 - Lang, H. A1 - Wirtz, K. A1 - Heitzer, M. T1 - Design by Analysis of Pressure Components by non-linear Optimization JF - The 10th International Conference on Pressure Vessel Technology, July 7-10, 2003, Vienna, Austria, Proceedings ICPVT-10 / Zeman, J. L. [ed] Y1 - 2003 SN - 3950152814 SP - 59 EP - 65 PB - ÖGS, Österreichische Gesellschaft für Schweißtechnik CY - Wien ER - TY - JOUR A1 - Staat, Manfred A1 - Heitzer, M. T1 - Limit and Shakedown Analysis with Uncertain Data JF - Stochastic optimization techniques : numerical methods and technical applications / Marti, K. [ed] Y1 - 2002 SN - 3-540-42889-5 SP - 241 EP - 254 PB - Springer CY - Heidelberg ER - TY - JOUR A1 - Ferrein, Alexander A1 - Mies, Christoph A1 - Lakemeyer, Gerhard T1 - Repairing Decision-Theoretic Policies Using Goal-Oriented Planning / Mies, Christoph ; Ferrein, Alexander ; Lakemeyer, Gerhard JF - KI 2008: Advances in Artificial Intelligence : 31st Annual German Conference on AI, KI 2008, Kaiserslautern, Germany, September 23-26, 2008. Proceedings Y1 - 2008 SN - 978-3-540-85844-7 N1 - Lecture Notes in Computer Science ; 5243 SP - 267 EP - 275 PB - Springer CY - Berlin ER - TY - JOUR A1 - Stadler, Andreas M. A1 - Zerlin, Kay A1 - Digel, Ilya A1 - Büldt, Georg A1 - Zaccai, Guiseppe A1 - Artmann, Gerhard T1 - Dynamics and interactions of hemoglobin in red blood cells JF - Tissue Engineering Part A. 14 (2008), H. 5 Y1 - 2008 SN - 1937-3341 N1 - TERMIS EU 2008 Porto Meeting June 22–26, 2008 Porto Congress Center–Alfândega Portugal SP - 724 EP - 724 ER -