TY - JOUR A1 - Öhlschläger, Peter A1 - Pes, Michaela A1 - Osen, Wolfram A1 - Dürst, Matthias T1 - An improved rearranged Human Papillomavirus Type 16 E7 DNA vaccine candidate (HPV-16 E7SH) induces an E7 wildtype-specific T cell response / Öhlschläger, Peter ; Pes, Michaela ; Osen, Wolfram ; Dürst, Matthias ; Schneider, Achim ; Gissmann, Lutz ; Kaufman JF - Vaccine. 24 (2006), H. 15 Y1 - 2006 SN - 0264-410X SP - 2880 EP - 2893 ER - TY - JOUR A1 - Bäcker, Matthias A1 - Raue, Markus A1 - Schusser, Sebastian A1 - Jeitner, C. A1 - Breuer, L. A1 - Wagner, P. A1 - Poghossian, Arshak A1 - Förster, Arnold A1 - Mang, Thomas A1 - Schöning, Michael Josef T1 - Microfluidic chip with integrated microvalves based on temperature- and pH-responsive hydrogel thin films JF - Physica Status Solidi (a) N2 - Two types of microvalves based on temperature-responsive poly(N-isopropylacrylamide) (PNIPAAm) and pH-responsive poly(sodium acrylate) (PSA) hydrogel films have been developed and tested. The PNIPAAm and PSA hydrogel films were prepared by means of in situ photopolymerization directly inside the fluidic channel of a microfluidic chip fabricated by combining Si and SU-8 technologies. The swelling/shrinking properties and height changes of the PNIPAAm and PSA films inside the fluidic channel were studied at temperatures of deionized water from 14 to 36 °C and different pH values (pH 3–12) of Titrisol buffer, respectively. Additionally, in separate experiments, the lower critical solution temperature (LCST) of the PNIPAAm hydrogel was investigated by means of a differential scanning calorimetry (DSC) and a surface plasmon resonance (SPR) method. Mass-flow measurements have shown the feasibility of the prepared hydrogel films to work as an on-chip integrated temperature- or pH-responsive microvalve capable to switch the flow channel on/off. Y1 - 2012 U6 - http://dx.doi.org/10.1002/pssa.201100763 SN - 1862-6319 VL - 209 IS - 5 SP - 839 EP - 845 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Aggarwal, P. A1 - Dhiman, S. A1 - Kumar, G. A1 - Scherer, Ulrich W. A1 - Singla, M. L. A1 - Srivastava, A. T1 - Optical study of poly(ethyleneterephthalate) modified by different ionizing radiation dose JF - Indian Journal of Pure and Applied Physics N2 - Thin films of poly(ethyleneterephthalate) [PET]were exposed to radiation dose ranging from 10 to 30 kGy by using gamma rays in the range 12.8-177.8 MGy using swift light ions of hydrogen. There was no effect of the radiation dose on the optical behaviour of PET as a result of exposure to radiation dose up to 30 kGy brought about by gamma rays but a significant decrease in the optical band gap values was observed when PET was exposed to swift light ions of hydrogen. The data obtained are discussed in terms of optical studies carried out on PET using swift heavy ions. Y1 - 2012 SN - 0019-5596 VL - 50 IS - 2 SP - 129 EP - 132 ER - TY - JOUR A1 - Werner, Frederik A1 - Groebel, Simone A1 - Krumbe, Christoph A1 - Wagner, Torsten A1 - Selmer, Thorsten A1 - Yoshinobu, Tatsuo A1 - Baumann, Marcus A1 - Schöning, Michael Josef T1 - Nutrient concentration-sensitive microorganism-based biosensor JF - Physica Status Solidi (a) Y1 - 2012 U6 - http://dx.doi.org/10.1002/pssa.201100801 SN - 1862-6319 VL - 209 IS - 5 SP - 900 EP - 904 PB - Wiley-VCH CY - Weinheim ER - TY - CHAP A1 - Feuerriegel, Uwe A1 - Wittenhorst, Simon A1 - Hoffmann, Ulrich A1 - Pook, Michael T1 - Simulation von Wärmeübertragungsprozessen T2 - Tagungsband zur AALE-Tagung 2012 : 9. Fachkonferenz Y1 - 2012 SN - 978-3-8356-3305-6 N1 - AALE 2012 SP - 127 EP - 136 PB - Oldenbourg Industrieverlag CY - München ER - TY - JOUR A1 - Schiffels, Johannes A1 - Pinkenburg, Olaf A1 - Schelden, Maximilian A1 - Aboulnaga, El-Hussiny A. A. A1 - Baumann, Marcus A1 - Selmer, Thorsten T1 - An innovative cloning platform enables large-scale production and maturation of an oxygen-tolerant [NiFe]-hydrogenase from cupriavidus necator in Escherichia coli JF - PLOS one. 2013 Y1 - 2013 U6 - http://dx.doi.org/10.1371/journal.pone.0068812 SN - 1932-6203 PB - Public Library of Science CY - San Francisco, California ER - TY - JOUR A1 - Abulnaga, El-Hussiny A1 - Pinkenburg, Olaf A1 - Schiffels, Johannes A1 - E-Refai, Ahmed A1 - Buckel, Wolfgang A1 - Selmer, Thorsten T1 - Effect of an Oxygen-Tolerant Bifurcating Butyryl Coenzyme A Dehydrogenase/Electron-Transferring Flavoprotein Complex from Clostridium difficile on Butyrate Production in Escherichia coli JF - Journal of bacteriology Y1 - 2013 SN - 1098-5530 [E-Journal] SN - 0021-9193 [Print] VL - 195 IS - 16 SP - 3704 EP - 3713 ER - TY - JOUR A1 - Aboulnaga, E. H. A1 - Pinkenburg, O. A1 - Schiffels, Johannes A1 - El-Refai, A. A1 - Buckel, W. A1 - Selmer, Thorsten T1 - Butyrate production in Escherichia coli: Exploitation of an oxygen tolerant bifurcating butyryl-CoA dehydrogenase/electron transferring flavoprotein complex from Clostridium difficile JF - Journal of bacteriology. June 14, 2013 Y1 - 2013 SN - 1098-5530 (E-Journal) ; 0021-9193 (Print) SP - Epub ahead of print ER - TY - JOUR A1 - Winckler, Silvia A1 - Krueger, Rolf A1 - Schnitzler, Thomas A1 - Zang, Werner A1 - Fischer, Rainer A1 - Biselli, Manfred T1 - A sensitive monitoring system for mammalian cell cultivation processes: a PAT approach JF - Bioprocess and biosystems engineering N2 - Biopharmaceuticals such as antibodies are produced in cultivated mammalian cells, which must be monitored to comply with good manufacturing practice. We, therefore, developed a fully automated system comprising a specific exhaust gas analyzer, inline analytics and a corresponding algorithm to precisely determine the oxygen uptake rate, carbon dioxide evolution rate, carbon dioxide transfer rate, transfer quotient and respiratory quotient without interrupting the ongoing cultivation, in order to assess its reproducibility. The system was verified using chemical simulation experiments and was able to measure the respiratory activity of hybridoma cells and DG44 cells (derived from Chinese hamster ovary cells) with satisfactory results at a minimum viable cell density of ~2.0 × 10⁵ cells ml⁻¹. The system was suitable for both batch and fed-batch cultivations in bubble-aerated and membrane-aerated reactors, with and without the control of pH and dissolved oxygen. Y1 - 2014 U6 - http://dx.doi.org/10.1007/s00449-013-1062-8 SN - 1615-7591 (Print) 1615-7605 (Online) VL - 37 IS - 5 SP - 901 EP - 912 PB - Springer CY - Berlin, Heidelberg ER - TY - JOUR A1 - Meyer-Stork, L. Sebastian A1 - Höcker, Hartwig A1 - Berndt, Heinz T1 - Syntheses and reactions of urethanes of cellobiose and cellulose-containing uretdione groups JF - Journal of applied polymer science Y1 - 1992 SN - 1097-4628 VL - 44 IS - 6 SP - 1043 EP - 1049 ER - TY - RPRT A1 - Vaeßen, Christiane A1 - Ohme, H. A1 - Manderscheid, D. T1 - Endbericht Projekt Immotherm : Vorhabensbezeichnung: KMU-innovativ-Verbundvorhaben "Einsatz immobilisierter Mikroorganismen zur Entölung und Entsalzung von Kondensatwasser bei hohen Prozesstemperaturen" : Laufzeit des Vorhabens: 01.03.2009-31.08.2011 : Förderkennzeichen: 01LY0816A, 01LY0816B, 01LY0816C Y1 - 2012 ER - TY - JOUR A1 - Schnitzler, Thomas T1 - Cultivation of hybridoma cell line CF-10H5 (DSMZ ACC477) JF - Application notes / Sartorius stedim biotech Y1 - 2009 SP - 1 EP - 4 ER - TY - JOUR A1 - Schnitzler, Thomas T1 - Kultivierung der Hybridomazelllinie CF-10H5 (DSMZ ACC477) JF - Application notes / Sartorius stedim biotech Y1 - 2009 PB - Sartorius Stedim Biotec CY - Aubagne ER - TY - JOUR A1 - Schnitzler, Thomas A1 - Biselli, Manfred T1 - Cultivo de lineas celulares de hibridoma por CF-10H5 (DSMZ ACC477) en el BIOSTAT B plus JF - Noticias tecnicas del laboratorio Y1 - 2005 VL - 13 IS - 3 SP - 7 EP - 8 ER - TY - JOUR A1 - Hemmerling, H.-J. A1 - Merschenz-Quack, Angelika A1 - Wunderlich, H. T1 - Crystal structures of indeno[1,2-d]imidazoles. XIth European Crystallographic Meeting, Vienna 1988 JF - Zeitschrift für Kristallographie - Crystalline Materials Y1 - 1988 SN - 2196-7105 (E-Books); 2194-4946 (Print) VL - 185 IS - H. 1-4 SP - 256 ER - TY - JOUR A1 - Mootz, D. A1 - Merschenz-Quack, Angelika T1 - Zur Kenntnis der höchsten Hydrate der Schwefelsäure: Bildung und Struktur von H2SO4·6,5 H2O und H2SO4·8H2O JF - Zeitschrift für Naturforschung B: Journal of chemical sciences Y1 - 1987 SN - 1865-7117 (E-Journal); 0932-0776 (Print) VL - 42 IS - H. 10 SP - 1231 EP - 1236 ER - TY - JOUR A1 - Merschenz-Quack, Angelika T1 - Betriebsanweisungen beim Umgang mit Gefahrstoffen JF - Elektro-Praktiker : Fachzeitschrift für Handwerk und Industrie Y1 - 1991 SN - 0013-5569 VL - Bd. 45 IS - H. 10 SP - 644 EP - 649 ER - TY - JOUR A1 - Gerigk, M. A1 - Maaß, D. A1 - Takors, Ralf A1 - Kreutzer, A. A1 - Wandrey, Christian A1 - Bongaerts, Johannes A1 - Wubbolts, Marcel T1 - Fermentative Herstellung von L-Phenylalanin im Fed-Batch Verfahren mit E. coli unter Einbindung eines integrierten Aufarbeitungsverfahrens JF - Chemie-Ingenieur-Technik (CIT) Y1 - 2000 SN - 1522-2640 (E-Journal); 0009-286X (Print) N1 - Printausg. in der Bibliothek vorhanden: 63 ZS 022 VL - Vol. 72 IS - Iss. 9 SP - 926 EP - 927 ER - TY - JOUR A1 - Müller, Ulrike A1 - Bongaerts, Johannes A1 - Bovenberg, Roel A1 - Jossek, Ralf A1 - Krämer, Marco A1 - Linnemann, J. A1 - Müschen, S. A1 - Ritterbecks, S. A1 - Sprenger, G. A1 - Wubbolts, Marcel T1 - Metabolic engineering to produce fine chemicals in Escherichia coli JF - Mededelingen van de Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent Y1 - 2001 SN - 0035-533x VL - 66 (3a) SP - 215 EP - 217 ER - TY - JOUR A1 - Jossek, Ralf A1 - Bongaerts, Johannes A1 - Sprenger, Georg A. T1 - Characterization of a new feedback-resistant 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase AroF of Escherichia coli JF - FEMS microbiology letters Y1 - 2001 SN - 1574-6968 VL - Vol. 202 IS - Iss. 1 SP - 145 EP - 148 ER -