TY  - JOUR
A1  - Stadler, Andreas M.
A1  - Garvey, G. J.
A1  - Bocahut, A.
A1  - Sacquin-Mora, S.
A1  - Digel, Ilya
A1  - Schneider, G. J.
A1  - Natali, F.
A1  - Artmann, Gerhard
A1  - Zaccai, G.
T1  - Thermal fluctuations of haemoglobin from different species : adaptation to temperature via conformational dynamics
JF  - Journal of the Royal Society Interface
N2  - Thermodynamic stability, configurational motions and internal forces of haemoglobin (Hb) of three endotherms (platypus, Ornithorhynchus anatinus; domestic chicken, Gallus gallus domesticus and human, Homo sapiens) and an ectotherm (salt water crocodile, Crocodylus porosus) were investigated using circular dichroism, incoherent elastic neutron scattering and coarse-grained Brownian dynamics simulations. The experimental results from Hb solutions revealed a direct correlation between protein resilience, melting temperature and average body temperature of the different species on the 0.1 ns time scale. Molecular forces appeared to be adapted to permit conformational fluctuations with a root mean square displacement close to 1.2 Å at the corresponding average body temperature of the endotherms. Strong forces within crocodile Hb maintain the amplitudes of motion within a narrow limit over the entire temperature range in which the animal lives. In fully hydrated powder samples of human and chicken, Hb mean square displacements and effective force constants on the 1 ns time scale showed no differences over the whole temperature range from 10 to 300 K, in contrast to the solution case. A complementary result of the study, therefore, is that one hydration layer is not sufficient to activate all conformational fluctuations of Hb in the pico- to nanosecond time scale which might be relevant for biological function. Coarse-grained Brownian dynamics simulations permitted to explore residue-specific effects. They indicated that temperature sensing of human and chicken Hb occurs mainly at residues lining internal cavities in the β-subunits.
Y1  - 2012
U6  - http://dx.doi.org/10.1098/rsif.2012.0364
SN  - 1742-5689
VL  - 9
IS  - 76
SP  - 2845
EP  - 2855
PB  - The Royal Society
CY  - London
ER  - 
TY  - JOUR
A1  - Stadler, Andreas M.
A1  - Embs, Jan P.
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Unruh, Tobias
A1  - Büldt, Georg
A1  - Zaccai, Guiseppe
T1  - Cytoplasmic water and hydration layer dynamics in human red blood cells
JF  - Journal of the American Chemical Society. 50 (2008), H. 130
Y1  - 2008
SN  - 1520-5126
SP  - 16852
EP  - 16853
ER  - 
TY  - JOUR
A1  - Stadler, Andreas M.
A1  - Digel, Ilya
A1  - Embs, Jan P.
A1  - Unruh, Tobias
A1  - Tehei, M.
A1  - Zaccai, G.
A1  - Büldt, G.
A1  - Artmann, Gerhard
T1  - From powder to solution : Hydration dependence of human hemoglobin dynamics correlated to body temperature
JF  - Biophysical Journal. 96 (2009), H. 12
Y1  - 2009
SN  - 0006-3495
SP  - 5073
EP  - 5081
PB  - Cell Press
CY  - Cambridge, Mass.
ER  - 
TY  - JOUR
A1  - Stadler, A.  M.
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Embs, Jan P.
A1  - Zaccai, Joe
A1  - Büldt, Georg
T1  - Hemoglobin Dynamics in Red Blood Cells: Correlation to Body Temperature
JF  - Biophysical Journal. 95 (2008), H. 11
Y1  - 2008
SN  - 1542-0086
SP  - 5449
EP  - 5461
ER  - 
TY  - JOUR
A1  - Savitskaya, Irina
A1  - Zhantlessova, Sirina
A1  - Kistaubayeva, Aida
A1  - Ignatova, Ludmila
A1  - Shokatayeva, Dina
A1  - Sinyavsky, Yuriy
A1  - Kushugulova, Almagul
A1  - Digel, Ilya
T1  - Prebiotic cellulose–pullulan matrix as a “vehicle” for probiotic biofilm delivery to the host large intestine
JF  - Polymers
N2  - This study describes the development of a new combined polysaccharide-matrix-based technology for the immobilization of Lactobacillus rhamnosus GG (LGG) bacteria in biofilm form. The new composition allows for delivering the bacteria to the digestive tract in a manner that improves their robustness compared with planktonic cells and released biofilm cells. Granules consisting of a polysaccharide matrix with probiotic biofilms (PMPB) with high cell density (>9 log CFU/g) were obtained by immobilization in the optimized nutrient medium. Successful probiotic loading was confirmed by fluorescence microscopy and scanning electron microscopy. The developed prebiotic polysaccharide matrix significantly enhanced LGG viability under acidic (pH 2.0) and bile salt (0.3%) stress conditions. Enzymatic extract of feces, mimicking colon fluid in terms of cellulase activity, was used to evaluate the intestinal release of probiotics. PMPB granules showed the ability to gradually release a large number of viable LGG cells in the model colon fluid. In vivo, the oral administration of PMPB granules in rats resulted in the successful release of probiotics in the colon environment. The biofilm-forming incubation method of immobilization on a complex polysaccharide matrix tested in this study has shown high efficacy and promising potential for the development of innovative biotechnologies.
KW  - immobilization
KW  - prebiotic
KW  - bacterial cellulose
KW  - biofilms
KW  - Lactobacillus rhamnosus GG
Y1  - 2023
U6  - http://dx.doi.org/10.3390/polym16010030
N1  - This article belongs to the Section "Polymer Composites and Nanocomposites"
IS  - 16(1)
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Savitskaya, I.S.
A1  - Kistaubayeva, A.S.
A1  - Ignatova, L.V.
A1  - Digel, Ilya
T1  - Antimicrobial and wound healing properties of a bacterial cellulose based material containing B. subtilis cells
JF  - Heliyon
Y1  - 2019
U6  - http://dx.doi.org/10.1016/j.heliyon.2019.e02592
SN  - 2405-8440
VL  - 5
IS  - 10
SP  - Artikelnummer e02592
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Savitskaya, I. S.
A1  - Kistaubayeva, A. S.
A1  - Digel, Ilya
A1  - Shokatayeva, D. H.
T1  - Physicochemical and Antibacterial Properties of Composite Films Based on Bacterial Cellulose and Chitosan for Wound Dressing Materials
JF  - Eurasian Chemico-Technological Journal
Y1  - 2017
U6  - http://dx.doi.org/10.18321/ectj670
SN  - 2522-4867
VL  - 19
IS  - 3
SP  - 255
EP  - 264
ER  - 
TY  - JOUR
A1  - Sagymbay, Altynay
A1  - G.E., Nusupbaeva
A1  - N.Zh, Tleumbetova
A1  - A.S., Mutalieva
A1  - Nurpeisova, Ainur
A1  - D.B., Jussupova
A1  - Digel, Ilya
T1  - Molecular genetics features of the epidemic season 2017-2018 on the influenza in Kazakhstan
JF  - Eurasian Journal of  Ecology
Y1  - 2019
SN  - 2617-7358
VL  - 58
IS  - 1
SP  - 50
EP  - 60
ER  - 
TY  - JOUR
A1  - Sadykov, Rustam
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
A1  - Porst, Dariusz
A1  - Linder, Peter
A1  - Kayser, Peter
A1  - Artmann, Gerhard
A1  - Savitskaya, Irina
A1  - Zhubanova, Azhar
T1  - Oral lead exposure induces dysbacteriosis in rats
JF  - Journal of Occupational Health. 51 (2009) (2009), H. 1
Y1  - 2009
SN  - 1348-9585
SP  - 64
EP  - 73
ER  - 
TY  - JOUR
A1  - Pogorelova, Natalia
A1  - Rogachev, Evgeniy
A1  - Digel, Ilya
A1  - Chernigova, Svetlana
A1  - Nardin, Dmitry
T1  - Bacterial Cellulose Nanocomposites: Morphology and Mechanical Properties
JF  - Materials
N2  - Bacterial cellulose (BC) is a promising material for biomedical applications due to its unique properties such as high mechanical strength and biocompatibility. This article describes the microbiological synthesis, modification, and characterization of the obtained BC-nanocomposites originating from symbiotic consortium Medusomyces gisevii. Two BC-modifications have been obtained: BC-Ag and BC-calcium phosphate (BC-Ca3(PO4)2). Structure and physicochemical properties of the BC and its modifications were investigated by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), atomic force microscopy (AFM), and infrared Fourier spectroscopy as well as by measurements of mechanical and water holding/absorbing capacities. Topographic analysis of the surface revealed multicomponent thick fibrils (150–160 nm in diameter and about 15 µm in length) constituted by 50–60 nm nanofibrils weaved into a left-hand helix. Distinctive features of Ca-phosphate-modified BC samples were (a) the presence of 500–700 nm entanglements and (b) inclusions of Ca3(PO4)2 crystals. The samples impregnated with Ag nanoparticles exhibited numerous roundish inclusions, about 110 nm in diameter. The boundaries between the organic and inorganic phases were very distinct in both cases. The Ag-modified samples also showed a prominent waving pattern in the packing of nanofibrils. The obtained BC gel films possessed water-holding capacity of about 62.35 g/g. However, the dried (to a constant mass) BC-films later exhibited a low water absorption capacity (3.82 g/g). It was found that decellularized BC samples had 2.4 times larger Young’s modulus and 2.2 times greater tensile strength as compared to dehydrated native BC films. We presume that this was caused by molecular compaction of the BC structure.
Y1  - 2020
SN  - 1996-1944
U6  - http://dx.doi.org/10.3390/ma13122849
VL  - 13
IS  - 12
SP  - 1
EP  - 16
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Pogorelova, Natalia
A1  - Rogachev, Evgeniy
A1  - Akimbekov, Nuraly
A1  - Digel, Ilya
T1  - Effect of dehydration method on the micro- and nanomorphological properties of bacterial cellulose produced by Medusomyces gisevii on different substrates
JF  - Journal of materials science
N2  - Many important properties of bacterial cellulose (BC), such as moisture absorption capacity, elasticity and tensile strength, largely depend on its structure. This paper presents a study on the effect of the drying method on BC films produced by Medusomyces gisevii using two different procedures: room temperature drying (RT, (24 ± 2 °C, humidity 65 ± 1%, dried until a constant weight was reached) and freeze-drying (FD, treated at − 75 °C for 48 h). BC was synthesized using one of two different carbon sources—either glucose or sucrose. Structural differences in the obtained BC films were evaluated using atomic force microscopy (AFM), scanning electron microscopy (SEM), and X-ray diffraction. Macroscopically, the RT samples appeared semi-transparent and smooth, whereas the FD group exhibited an opaque white color and sponge-like structure. SEM examination showed denser packing of fibrils in FD samples while RT-samples displayed smaller average fiber diameter, lower surface roughness and less porosity. AFM confirmed the SEM observations and showed that the FD material exhibited a more branched structure and a higher surface roughness. The samples cultivated in a glucose-containing nutrient medium, generally displayed a straight and ordered shape of fibrils compared to the sucrose-derived BC, characterized by a rougher and wavier structure. The BC films dried under different conditions showed distinctly different crystallinity degrees, whereas the carbon source in the culture medium was found to have a relatively small effect on the BC crystallinity.
Y1  - 2024
U6  - http://dx.doi.org/10.1007/s10853-024-09596-3
SN  - 1573-4803 (Online)
SN  - 0022-2461 (Print)
N1  - Corresponding author: Ilya Digel
VL  - 2024
PB  - Springer Science + Business Media
CY  - Dordrecht
ER  - 
TY  - JOUR
A1  - Micili, Serap C.
A1  - Valter, Markus
A1  - Oflaz, Hakan
A1  - Ozogul, Candan
A1  - Linder, Peter
A1  - Föckler, Nicole
A1  - Artmann, Gerhard
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
T1  - Optical coherence tomography : a potential tool to predict premature rupture of fetal membranes
JF  - Proceedings of the Institution of Mechanical Engineers. Part H : Journal of engineering in medicine
Y1  - 2013
SN  - 0046-2039 (Print) ; 2041-3033 (E-Journal)
VL  - Vol. 227
IS  - No. 4
SP  - 393
EP  - 401
PB  - Sage
CY  - London
ER  - 
TY  - JOUR
A1  - Mansurov, Z.
A1  - Digel, Ilya
A1  - Biisenbaev, M.
A1  - Savistkaya, I.
A1  - Kistaubaeva, A.
A1  - Akimbekov, N.
A1  - Zhubanova, A.
T1  - Bio-composite material on the basis of carbonized rice husk in biomedicine and environmental applications
JF  - Eurasian Chemico-Technological Journal
Y1  - 2012
U6  - http://dx.doi.org/10.18321/ectj105
SN  - 2522-4867
VL  - 14
IS  - 2
SP  - 115
EP  - 131
PB  - Institute of Combustion Problems
CY  - Almaty
ER  - 
TY  - JOUR
A1  - Mansurov, Z. A.
A1  - Jandosov, J. M.
A1  - Kerimkulova, A. R.
A1  - Azat, S.
A1  - Zhubanova, A. A.
A1  - Digel, Ilya
A1  - Savistkaya, I. S.
A1  - Akimbekov, N. S.
A1  - Kistaubaeva, A. S.
T1  - Nanostructured carbon materials for biomedical use
JF  - Eurasian chemico-technological journal : quarterly journal of the International Higher Education Academy of Sciences
N2  - One of the priority trends of carbon nanotechnology is creation of nanocomposite systems. Such carbon nanostructured composites were produced using - raw materials based on the products of agricultural waste, such as grape stones, apricot stones, rice husk. These products have a - wide spectrum of application and can be obtained in large quantities. The Institute of Combustion Problems has carried out the work on synthesis of the nanostructured carbon sorbents for multiple applications including the field of biomedicine. The article presents the data on the synthesis and physico-chemical properties of carbonaceous sorbents using physicochemical methods of investigation: separation and purification of biomolecules; isolation of phytohormone - fusicoccin; adsorbent INGO-1 in the form of an adsorption column for blood detoxification, oral (entero) sorbent - INGO-2; the study of efferent and probiotic properties and sorption activity in regard to the lipopolysaccharide (LPS), new biocomposites - based on carbonized rice husk (CRH) and cellular microorganisms; the use of CRH in wound treatment. A new material for blood detoxication (INGO-1) has been obtained. Adsorption of p-cresyl sulfate and indoxyl sulfate has shown that active carbon adsorbent can remove clinically significant level of p-cresyl sulfate and indoxyl sulfate from human plasma. Enterosorbent INGO-2 possesses high adsorption activity in relation to Gram-negative bacteria and their endotoxins. INGO-2 slows down the growth of conditionally pathogenic microorganisms, without having a negative effect on bifido and lactobacteria. The use of enterosorbent INGO-2 for sorption therapy may provide a solution to a complex problem - detoxication of the digestive tract and normalization of the intestinal micro ecology. The immobilized probiotic called "Riso-lact" was registered at the Ministry of Health of the Republic of Kazakhstan as a biologically active food additive. The developed technology is patented and provides production of the medicine in the form of freeze-dried biomass immobilized in vials.
Y1  - 2014
U6  - http://dx.doi.org/10.18321/ectj224
SN  - 1562-3920
VL  - 15 (2013)
IS  - 3
SP  - 209
EP  - 217
PB  - Institute of Combustion Problems
CY  - Almaty
ER  - 
TY  - JOUR
A1  - Maggakis-Kelemen, Christina
A1  - Digel, Ilya
A1  - Artmann, Gerhard
T1  - Polystyrene sulfonate/Polyallylamine hydrochloride microcapsules as potential artificial red blood cells - improvement of capsule flexibility
JF  - Biomedizinische Technik. 50 (2005), H. Erg.-Bd. 1
Y1  - 2005
SP  - 324
EP  - 326
ER  - 
TY  - JOUR
A1  - Linder, Peter
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
A1  - Kayser, Peter
A1  - Porst, Dariusz
A1  - Artmann, Gerhard
T1  - High-throughput testing of mechanical forces generated in thin cell and tissue layers
JF  - Tissue Engineering. 13 (2007), H. 7
Y1  - 2007
SN  - 1076-3279
N1  - Meeting abstract 433
SP  - 1778
EP  - 1778
ER  - 
TY  - JOUR
A1  - Leimena, W.
A1  - Artmann, Gerhard
A1  - Dachwald, Bernd
A1  - Temiz Artmann, Aysegül
A1  - Gossmann, Matthias
A1  - Digel, Ilya
T1  - Feasibility of an in-situ microbial decontamination of an ice-melting probe
JF  - Eurasian Chemico-Technological Journal. 12 (2010), H. 2
Y1  - 2010
SN  - 1562-3920
SP  - 145
EP  - 150
ER  - 
TY  - JOUR
A1  - Kurz, R.
A1  - Linder, Peter
A1  - Trzewik, Jürgen
A1  - Rüffer, M.
A1  - Artmann, Gerhard
A1  - Digel, Ilya
A1  - Rothermel, A.
A1  - Robitzki, A.
A1  - Temiz Artmann, Aysegül
T1  - Contractile tension and beating rates of self-exciting monolayers and 3D-tissue constructs of neonatal rat cardiomyocytes
JF  - Medical and Biological Engineering and Computing
N2  - The CellDrum technology (The term 'CellDrum technology' includes a couple of slightly different technological setups for measuring lateral mechanical tension in various types of cell monolayers or 3D-tissue constructs) was designed to quantify the contraction rate and mechanical tension of self-exciting cardiac myocytes. Cells were grown either within flexible, circular collagen gels or as monolayer on top of respective 1-mum thin silicone membranes. Membrane and cells were bulged outwards by air pressure. This biaxial strain distribution is rather similar the beating, blood-filled heart. The setup allowed presetting the mechanical residual stress level externally by adjusting the centre deflection, thus, mimicking hypertension in vitro. Tension was measured as oscillating differential pressure change between chamber and environment. A 0.5-mm thick collagen-cardiac myocyte tissue construct induced after 2 days of culturing (initial cell density 2 x 10(4) cells/ml), a mechanical tension of 1.62 +/- 0.17 microN/mm(2). Mechanical load is an important growth regulator in the developing heart, and the orientation and alignment of cardiomyocytes is stress sensitive. Therefore, it was necessary to develop the CellDrum technology with its biaxial stress-strain distribution and defined mechanical boundary conditions. Cells were exposed to strain in two directions, radially and circumferentially, which is similar to biaxial loading in real heart tissues. Thus, from a biomechanical point of view, the system is preferable to previous setups based on uniaxial stretching.
Y1  - 2010
U6  - http://dx.doi.org/10.1007/s11517-009-0552-y
SN  - 1741-0444
VL  - 48
IS  - 1
SP  - 59
EP  - 65
PB  - Springer Nature
CY  - Cham
ER  - 
TY  - JOUR
A1  - Kurulgan Demirci, Eylem
A1  - Linder, Peter
A1  - Demirci, Taylan
A1  - Trzewik, Jürgen
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Contractile tension of endothelial cells: An LPS based in-vitro sepsis model
JF  - IUBMB Life. 61 (2009), H. 3
Y1  - 2009
SN  - 1521-6543
N1  - Abstracts: Turkish Society of Molecular Medicine, Third International Congress of Molecular Medicine, May 5-8, 2009, Istanbul, Turkey
SP  - 307
EP  - 308
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Kurulgan Demirci, Eylem
A1  - Demirci, Taylan
A1  - Linder, Peter
A1  - Trzewik, Jürgen
A1  - Gierkowski, Jessica Ricarda
A1  - Gossmann, Matthias
A1  - Kayser, Peter
A1  - Porst, Dariusz
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - rhAPC reduces the endothelial cell permeability via a decrease of contractile tensions induced by endothelial cells
JF  - Journal of Bioscience and Bioengineering
N2  - All cells generate contractile tension. This strain is crucial for mechanically controlling the cell shape, function and survival. In this study, the CellDrum technology quantifying cell's (the cellular) mechanical tension on a pico-scale was used to investigate the effect of lipopolysaccharide (LPS) on human aortic endothelial cell (HAoEC) tension. The LPS effect during gram-negative sepsis on endothelial cells is cell contraction causing endothelium permeability increase. The aim was to finding out whether recombinant activated protein C (rhAPC) would reverse the endothelial cell response in an in-vitro sepsis model. In this study, the established in-vitro sepsis model was confirmed by interleukin 6 (IL-6) levels at the proteomic and genomic levels by ELISA, real time-PCR and reactive oxygen species (ROS) activation by florescence staining. The thrombin cellular contraction effect on endothelial cells was used as a positive control when the CellDrum technology was applied. Additionally, the Ras homolog gene family, member A (RhoA) mRNA expression level was checked by real time-PCR to support contractile tension results. According to contractile tension results, the mechanical predominance of actin stress fibers was a reason of the increased endothelial contractile tension leading to enhanced endothelium contractility and thus permeability enhancement. The originality of this data supports firstly the basic measurement principles of the CellDrum technology and secondly that rhAPC has a beneficial effect on sepsis influenced cellular tension. The technology presented here is promising for future high-throughput cellular tension analysis that will help identify pathological contractile tension responses of cells and prove further cell in-vitro models.
KW  - Cell permeability
KW  - Cellular force
KW  - Endothelial cells
KW  - Recombinant activated protein C
KW  - Lipopolysaccharide
KW  - Contractile tension
KW  - CellDrum
Y1  - 2012
U6  - http://dx.doi.org/10.1016/j.jbiosc.2012.03.019
SN  - 1347-4421
VL  - 113
IS  - 2
SP  - 212
EP  - 219
PB  - Elsevier
CY  - Amsterdam
ER  -