TY - JOUR A1 - Falkenberg, Fabian A1 - Bott, Michael A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from Bacillaceae JF - Frontiers in Microbiology N2 - The subtilase family (S8), a member of the clan SB of serine proteases are ubiquitous in all kingdoms of life and fulfil different physiological functions. Subtilases are divided in several groups and especially subtilisins are of interest as they are used in various industrial sectors. Therefore, we searched for new subtilisin sequences of the family Bacillaceae using a data mining approach. The obtained 1,400 sequences were phylogenetically classified in the context of the subtilase family. This required an updated comprehensive overview of the different groups within this family. To fill this gap, we conducted a phylogenetic survey of the S8 family with characterised holotypes derived from the MEROPS database. The analysis revealed the presence of eight previously uncharacterised groups and 13 subgroups within the S8 family. The sequences that emerged from the data mining with the set filter parameters were mainly assigned to the subtilisin subgroups of true subtilisins, high-alkaline subtilisins, and phylogenetically intermediate subtilisins and represent an excellent source for new subtilisin candidates. Y1 - 2022 U6 - http://dx.doi.org/10.3389/fmicb.2022.1017978 SN - 1664-302X VL - 2022 IS - 13 PB - Frontiers CY - Lausanne ER - TY - BOOK A1 - Lauth, Jakob T1 - Physical chemistry in a nutshell: Basics for engineers and scientists N2 - This book is based on a multimedia course for biological and chemical engineers, which is designed to trigger students' curiosity and initiative. A solid basic knowledge of thermodynamics and kinetics is necessary for understanding many technical, chemical, and biological processes. The one-semester basic lecture course was divided into 12 workshops (chapters). Each chapter covers a practically relevant area of physical chemistry and contains the following didactic elements that make this book particularly exciting and understandable: - Links to Videos at the start of each chapter as preparation for the workshop - Key terms (in bold) for further research of your own - Comprehension questions and calculation exercises with solutions as learning checks - Key illustrations as simple, easy-to-replicate blackboard pictures Humorous cartoons for each workshop (by Faelis) additionally lighten up the text and facilitate the learning process as a mnemonic. To round out the book, the appendix includes a summary of the most popular experiments in basic physical chemistry courses, as well as suggestions for designing workshops with exhibits, experiments, and "questions of the day." Suitable for students minoring in chemistry; chemistry majors are sure to find this slimmed-down, didactically valuable book helpful as well. The book is excellent for self-study. KW - Physical chemistry KW - Thermodynamics as minor KW - Physical chemistry starters KW - Minor chemistry KW - Physical chemistry basics Y1 - 2023 SN - 978-3-662-67636-3 (Softcover) SN - 978-3-662-67637-0 (eBook) U6 - http://dx.doi.org/10.1007/978-3-662-67637-0 PB - Springer CY - Berlin ER - TY - JOUR A1 - Aggarwal, Prerna A1 - Singh, Virendra A1 - Singh, Arjun A1 - Scherer, Ulrich W. A1 - Singh, Tejvir A1 - Singla, Madan L. A1 - Srivastava, Alok T1 - Physico-chemical transformations in swift heavy ion modified poly(ethyleneterephthalate) JF - Radiation Physics and Chemistry Y1 - 2011 SN - 0969-806X N1 - In Press, Accepted Manuscript SP - 1 EP - 28 PB - Pergamon Press CY - Oxford ER - TY - JOUR A1 - Smith, Walker O. A1 - Baumann, Marcus A1 - Wilson, David L. A1 - Aletsee, Ludwig T1 - Phytoplankton biomass and productivity in the marginal ice zone of the Fram Strait during summer 1984 JF - Journal of geophysical research Y1 - 1987 SN - 2156-2202 (E-Journal); 2169-9291 (E-Journal); 0148-0227 (Print); 2169-9275 (Print) VL - Vol. 92 IS - Iss. C7 SP - 6777 EP - 6786 ER - TY - JOUR A1 - Baumann, Marcus A1 - Goeyens, L. A1 - Semeneh, M. A1 - Elskens, M. T1 - Phytoplankton nutrient utilisation and nutrient signature in the Southern Ocean / Goeyens, L. ; Semeneh, M. ; Baumann, M.E.M. ; Elskens, M. ; Shopova, D. ; Dehairs, F. JF - Journal of Marine Systems. 17 (1998), H. 1-4 Y1 - 1998 SN - 0924-7963 SP - 143 EP - 157 ER - TY - JOUR A1 - Biselli, Manfred A1 - Zeng, Steffen A1 - Dinter, Andre A1 - Eisenkrätzer, Detlef T1 - Pilot Scale Expression and Purification of Soluble Protein A Tagged β1,6N-Acetylglucosaminyltransferase in CHO Cells / Zeng, Steffen ; Dinter, Andre ; Eisenkrätzer, Detlef ; Biselli, Manfred ; Wandrey, Christian ; Berger, Eric G. JF - Biochemical and Biophysical Research Communications. 237 (1997), H. 3 Y1 - 1997 SN - 0006-291X SP - 653 EP - 658 ER - TY - JOUR A1 - Baumann, Marcus A1 - Hirche, H.-J. A1 - Kattner, G. A1 - Gradinger, R. T1 - Plankton distribution and the impact of copepod grazing on primary production in Fram Strait, Greenland Sea / Hirche, H.-J. ; Baumann, M.E.M. ; Kattner, G.; Gradinger, R. JF - Journal of Marine Systems. 2 (1991), H. 3-4 Y1 - 1991 SN - 0924-7963 SP - 477 EP - 494 ER - TY - JOUR A1 - Michalak, Ewa Malgorzata A1 - Nacerddine, Karim A1 - Pietersen, Alexandra A1 - Beuger, Vincent A1 - Pawlitzky, Inka A1 - Cornelissen-Steijger, Paulien A1 - Wientjens, Ellen A1 - Tanger, Ellen A1 - Seibler, Jost A1 - Lohuizen, Maarten van A1 - Jonkers, Jos T1 - Polycomb group gene Ezh2 regulates mammary gland morphogenesis and maintains the luminal progenitor pool JF - Stem Cells Y1 - 2013 U6 - http://dx.doi.org/10.1002/stem.1437 SN - 1549-4918 VL - Vol 31 IS - 9 SP - 1910 EP - 1920 PB - Oxford University Press CY - Oxford ER - TY - JOUR A1 - Mang, Thomas A1 - Kricheldorf, Hans R. A1 - Jonté, J. Michael T1 - Polylactones, 2. Copolymerization of glycolide with β-propiolactone, χ-butyrolactone or δ-valerolactone / Kricheldorf, Hans R. ; Mang, Thomas ; Jonté, J. Michael JF - Die makromolekulare Chemie. 186 (1985), H. 5 Y1 - 1985 SN - 1022-1352 SP - 955 EP - 976 ER - TY - JOUR A1 - Mang, Thomas A1 - Kricheldorf, Hans R. A1 - Jonté, J. Michael T1 - Polylactones. 1. Copolymerizations of glycolide and &beta-caprolactone / Kricheldorf, Hans R. ; Mang, Thomas ; Jonté, J. Michael JF - Macromolecules. 17 (1984), H. 10 Y1 - 1984 SN - 0024-9297 SP - 2173 EP - 2181 ER - TY - JOUR A1 - Mang, Thomas T1 - Polymer Magnetic Particles for Isolating Biomolecules, Especially Nucleic Acids JF - Biomedizinische Technik = Biomedical Engineering. 49 (2004), H. 2 Y1 - 2004 SN - 0013-5585 SP - 1008 EP - 1009 ER - TY - JOUR A1 - Oosterhuis, Koen A1 - Öhlschläger, Peter A1 - Berg, Joost H. van den A1 - Toebes, Mireille A1 - Gomez, Raquel A1 - Schumacher, Ton N. A1 - Haanen, John B. T1 - Preclinical development of highly effective and safe DNA vaccines directed against HPV 16 E6 and E7 JF - International Journal of Cancer Y1 - 2011 SN - 1097-0215 VL - 129 IS - 2 SP - 397 EP - 406 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Henken, F. E. A1 - Oosterhuis, K. A1 - Öhlschläger, Peter A1 - Bosch, L. A1 - Hooijberg, E. A1 - Haanen, J. B. A. G. A1 - Steenbergen, R. D. M. T1 - Preclinical safety evaluation of DNA vaccines encoding modified HPV16 E6 and E7 JF - Vaccine N2 - Persistent infection with high-risk human papillomaviruses (hrHPV) can result in the formation of anogenital cancers. As hrHPV proteins E6 and E7 are required for cancer initiation and maintenance, they are ideal targets for immunotherapeutic interventions. Previously, we have described the development of DNA vaccines for the induction of HPV16 E6 and E7 specific T cell immunity. These vaccines consist of ‘gene-shuffled’ (SH) versions of HPV16 E6 and E7 that were fused to Tetanus Toxin Fragment C domain 1 (TTFC) and were named TTFC-E6SH and TTFC-E7SH. Gene-shuffling was performed to avoid the risk of inducing malignant transformation at the vaccination site. Here, we describe the preclinical safety evaluation of these candidate vaccines by analysis of their transforming capacity in vitro using established murine fibroblasts (NIH 3T3 cells) and primary human foreskin keratinocytes (HFKs). We demonstrate that neither ectopic expression of TTFC-E6SH and TTFC-E7SH alone or in combination enabled NIH 3T3 cells to form colonies in soft agar. In contrast, expression of HPV16 E6WT and E7WT alone or in combination resulted in effective transformation. Similarly, retroviral transduction of HFKs from three independent donors with both TTFC-E6SH and TTFC-E7SH alone or in combination did not show any signs of immortalization. In contrast, the combined expression of E6WT and E7WT induced immortalization in HFKs from all donors. Based on these results we consider it justified to proceed to clinical evaluation of DNA vaccines encoding TTFC-E6SH and TTFC-E7SH in patients with HPV16 associated (pre)malignancies. Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.vaccine.2012.04.013 SN - 0264-410X VL - 30 IS - 28 SP - 4259 EP - 4266 PB - Elsevier CY - Amsterdam ER - TY - BOOK A1 - Lauth, Jakob A1 - Harreus, Albrecht A1 - Götz, Norbert A1 - Rang, Harald [u.a.] T1 - Preparation O-(2-Hydroxyalkyl)Oximes = of Verfahren zur Herstellung von O-(2-Hydroxy)Oximen : Europäische Patentschrift EP0775107B1 ; Veröffentlichungstag im Patentblatt: 18.11.1999 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Albrecht Harreus ; Norbert Götz ; Harald Rang ... Günter Lauth Y1 - 1999 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ ; Text in engl. ; Deutsche Übersetzung unter DE69512786T2 ; Internationale Veröffentlichungsnummer: WO 96/04238 PB - Europäisches Patentamt CY - [München u.a.] ER - TY - PAT A1 - Lauth, Jakob A1 - Wolf, Gerhard A1 - Burkhart, Bernd T1 - Preparation of alkoxylation products in the presence of mixed hydroxides modified with additives : United States Patent ; patent number 5,741,947 ; date of patent: Apr. 21, 1998 / assignee: BASF Aktiengesellschaft. Inventors: Gerhard Wolf ; Bernd Burkhart ; Guenter Lauth ... Y1 - 1998 N1 - Barcode: US005741947A ; Volltext über Datenbank: http://www.google.com/patents PB - [United States Trademark and Patent Office] CY - [Alexandria, VA] ER - TY - PAT A1 - Lauth, Jakob A1 - Müller, Ulrich A1 - Hoelderich, Wolfgang T1 - Preparation of aluminophosphates and silicoaluminophosphates having the AEL structure using 1,2-bis-( 4-pyridyl)-ethane : United States Patent ; patent number 5,324,493 ; date of patent: Jun. 28, 1994 / assignee: BASF Aktiengesellschaft. Inventors: Ulrich Mueller ; Wolfgang Hoelderich ; Guenter Lauth Y1 - 1994 N1 - Barcode: US005324493A ; Volltext über Datenbank: http://www.google.com/patents PB - [United States Trademark and Patent Office] CY - [Alexandria, VA] ER - TY - PAT A1 - Lauth, Jakob A1 - Dingerdissen, Uwe A1 - Henne, Andreas T1 - Preparation of amines : United States Patent ; patent number 5,817,871 ; date of patent: Oct. 6, 1998 / Assignee: BASF Aktiengesellschaft. Inventors: Uwe Dingerdissen ; Gunter Lauth ; Andreas Henne .... Y1 - 1998 N1 - Barcode: US005817871A ; Volltext über Datenbank: http://www.google.com/patents ; Falsche Namensansetzung: Gunter ... PB - [United States Trademark and Patent Office] CY - [Alexandria, VA] ER - TY - PAT A1 - Lauth, Jakob A1 - Dingerdissen, Uwe A1 - Trübenbach, Peter A1 - Steuerle, Ulrich T1 - Preparation of aziridines : United States Patent ; patent number 5,693,825 ; date of patent: Dec. 2, 1997 / assignee: BASF Aktiengesellschaft. Inventors: Uwe Dingerdissen ; Gunter Lauth ; Peter Trubenbach ; Ulrich Steuerle Y1 - 1997 N1 - Barcode: US005324493A ; Volltext über Datenbank: http://www.google.com/patents ; Falsche Namensansetzung in Datenbank: Gunter ... PB - United States Patent and Trademark Office CY - [Alexandria, VA] ER - TY - JOUR A1 - Biselli, Manfred A1 - Schmid, Georg A1 - Wandrey, Christian T1 - Preparation of cellodextrins and isolation of oligomeric side components and their characterization / Schmid, Georg ; Biselli, Manfred ; Wandrey, Christian JF - Analytical Biochemistry. 175 (1988) Y1 - 1988 SN - 0003-2697 SP - 573 EP - 583 ER - TY - PAT A1 - Lauth, Jakob A1 - Harreus, Albrecht A1 - Goetz, Norbert A1 - Rang, Harald T1 - Preparation of O-(2-hydroxyalkyl) oximes : [South African Patent ] publication number: ZA9506402 ; publication date: 1997-02-03 / applicant: BASF AG. Inventor: Harreus, Albrecht ; Goetz, Norbert ; Rang, Harald .... Lauth, Guenter ... Y1 - 1997 N1 - Also published as WO9604238 PB - [South African Companies and Intellectual Property Registration Office] CY - [Pretoria] ER -