TY - JOUR A1 - Lapitan, Denis G. A1 - Rogatkin, Dmitrii A. A1 - Persheyev, Sydulla K. A1 - Kotliar, Konstantin T1 - False spectra formation in the differential two-channel scheme of the laser Doppler flowmeter JF - Biomedizinische Technik N2 - Noise in the differential two-channel scheme of a classic laser Doppler flowmetry (LDF) instrument was studied. Formation of false spectral components in the output signal due to beating of electrical signals in the differential amplifier was found out. The improved block-diagram of the flowmeter was developed allowing to reduce the noise. Y1 - 2018 U6 - http://dx.doi.org/10.1515/bmt-2017-0060 SN - 0013-5585 VL - 63 IS - 4 SP - 439 EP - 444 PB - De Gruyter CY - Berlin ER - TY - JOUR A1 - Müller, Janina A1 - Beckers, Mario A1 - Mußmann, Nina A1 - Bongaerts, Johannes A1 - Büchs, Jochen T1 - Elucidation of auxotrophic deficiencies of Bacillus pumilus DSM 18097 to develop a defined minimal medium JF - Microbial Cell Factories N2 - Background Culture media containing complex compounds like yeast extract or peptone show numerous disadvantages. The chemical composition of the complex compounds is prone to significant variations from batch to batch and quality control is difficult. Therefore, the use of chemically defined media receives more and more attention in commercial fermentations. This concept results in better reproducibility, it simplifies downstream processing of secreted products and enable rapid scale-up. Culturing bacteria with unknown auxotrophies in chemically defined media is challenging and often not possible without an extensive trial-and-error approach. In this study, a respiration activity monitoring system for shake flasks and its recent version for microtiter plates were used to clarify unknown auxotrophic deficiencies in the model organism Bacillus pumilus DSM 18097. Results Bacillus pumilus DSM 18097 was unable to grow in a mineral medium without the addition of complex compounds. Therefore, a rich chemically defined minimal medium was tested containing basically all vitamins, amino acids and nucleobases, which are essential ingredients of complex components. The strain was successfully cultivated in this medium. By monitoring of the respiration activity, nutrients were supplemented to and omitted from the rich chemically defined medium in a rational way, thus enabling a systematic and fast determination of the auxotrophic deficiencies. Experiments have shown that the investigated strain requires amino acids, especially cysteine or histidine and the vitamin biotin for growth. Conclusions The introduced method allows an efficient and rapid identification of unknown auxotrophic deficiencies and can be used to develop a simple chemically defined tailor-made medium. B. pumilus DSM 18097 was chosen as a model organism to demonstrate the method. However, the method is generally suitable for a wide range of microorganisms. By combining a systematic combinatorial approach based on monitoring the respiration activity with cultivation in microtiter plates, high throughput experiments with high information content can be conducted. This approach facilitates media development, strain characterization and cultivation of fastidious microorganisms in chemically defined minimal media while simultaneously reducing the experimental effort. Y1 - 2018 U6 - http://dx.doi.org/10.1186/s12934-018-0956-1 SN - 1475-2859 VL - 17 IS - 1 SP - Article No. 106 PB - BioMed Central ER - TY - CHAP A1 - Bung, Daniel B. A1 - Valero, Daniel A1 - Hermens, G. T1 - Hybrid investigation on the hydraulic performance of a new trapezoidal fishway T2 - 7th IAHR International Symposium on Hydraulic Structures, ISHS 2018 Y1 - 2018 SN - 978-069213277-7 U6 - http://dx.doi.org/10.15142/T3S06R SP - 184 EP - 193 ER - TY - CHAP A1 - Fissabre, Anke ED - Priesterjahn, Maike T1 - Vom Schiff an Land oder von der Muschel zur Rocaille T2 - Schwimmender Barock : Das Schiff als Repräsentationsobjekt Y1 - 2018 SN - 978-3-89809-153-4 SP - 59 EP - 65 PB - Bebra Verlag CY - Berlin ER - TY - CHAP A1 - Pieper, Martin T1 - Digitale Hochschullehre in mathematischen und mathematikdidaktischen Veranstaltungen T2 - Beiträge zum Mathematikunterricht 2018 : Vorträge zur Mathematikdidaktik und zur Schnittstelle Mathematik/Mathematikdidaktik auf der gemeinsamen Jahrestagung GDM und DMV 2018 (52. Jahrestagung der Gesellschaft für Didaktik der Mathematik). Bd. 1 Y1 - 2018 SN - 978-3-95987-089-4 SP - 105 EP - 106 PB - WTM-Verlag CY - Münster ER - TY - CHAP A1 - Pieper, Martin T1 - Lernzielorientierte Kurse und Stack Aufgaben in der Mathematikausbildung T2 - Beiträge zum Mathematikunterricht 2018 : Vorträge zur Mathematikdidaktik und zur Schnittstelle Mathematik/Mathematikdidaktik auf der gemeinsamen Jahrestagung GDM und DMV 2018 (52. Jahrestagung der Gesellschaft für Didaktik der Mathematik). Bd. 3 Y1 - 2018 SN - 978-3-95987-089-4 SP - 1399 EP - 1402 PB - WTM-Verlag CY - Münster ER - TY - JOUR A1 - Röhlen, Desiree A1 - Pilas, Johanna A1 - Dahmen, Markus A1 - Keusgen, Michael A1 - Selmer, Thorsten A1 - Schöning, Michael Josef T1 - Toward a Hybrid Biosensor System for Analysis of Organic and Volatile Fatty Acids in Fermentation Processes JF - Frontiers in Chemistry N2 - Monitoring of organic acids (OA) and volatile fatty acids (VFA) is crucial for the control of anaerobic digestion. In case of unstable process conditions, an accumulation of these intermediates occurs. In the present work, two different enzyme-based biosensor arrays are combined and presented for facile electrochemical determination of several process-relevant analytes. Each biosensor utilizes a platinum sensor chip (14 × 14 mm²) with five individual working electrodes. The OA biosensor enables simultaneous measurement of ethanol, formate, d- and l-lactate, based on a bi-enzymatic detection principle. The second VFA biosensor provides an amperometric platform for quantification of acetate and propionate, mediated by oxidation of hydrogen peroxide. The cross-sensitivity of both biosensors toward potential interferents, typically present in fermentation samples, was investigated. The potential for practical application in complex media was successfully demonstrated in spiked sludge samples collected from three different biogas plants. Thereby, the results obtained by both of the biosensors were in good agreement to the applied reference measurements by photometry and gas chromatography, respectively. The proposed hybrid biosensor system was also used for long-term monitoring of a lab-scale biogas reactor (0.01 m³) for a period of 2 months. In combination with typically monitored parameters, such as gas quality, pH and FOS/TAC (volatile organic acids/total anorganic carbonate), the amperometric measurements of OA and VFA concentration could enhance the understanding of ongoing fermentation processes. Y1 - 2018 U6 - http://dx.doi.org/10.3389/fchem.2018.00284 IS - 6 PB - Frontiers CY - Lausanne ER - TY - JOUR A1 - Rodrigues, Raul T. A1 - Morais, Paulo V. A1 - Nordi, Cristina S. F. A1 - Schöning, Michael Josef A1 - Siqueira Jr., José R. A1 - Caseli, Luciano T1 - Carbon Nanotubes and Algal Polysaccharides To Enhance the Enzymatic Properties of Urease in Lipid Langmuir-Blodgett Films JF - Langmuir N2 - Algal polysaccharides (extracellular polysaccharides) and carbon nanotubes (CNTs) were adsorbed on dioctadecyldimethylammonium bromide Langmuir monolayers to serve as a matrix for the incorporation of urease. The physicochemical properties of the supramolecular system as a monolayer at the air–water interface were investigated by surface pressure–area isotherms, surface potential–area isotherms, interfacial shear rheology, vibrational spectroscopy, and Brewster angle microscopy. The floating monolayers were transferred to hydrophilic solid supports, quartz, mica, or capacitive electrolyte–insulator–semiconductor (EIS) devices, through the Langmuir–Blodgett (LB) technique, forming mixed films, which were investigated by quartz crystal microbalance, fluorescence spectroscopy, and field emission gun scanning electron microscopy. The enzyme activity was studied with UV–vis spectroscopy, and the feasibility of the thin film as a urea sensor was essayed in an EIS sensor device. The presence of CNT in the enzyme–lipid LB film not only tuned the catalytic activity of urease but also helped to conserve its enzyme activity. Viability as a urease sensor was demonstrated with capacitance–voltage and constant capacitance measurements, exhibiting regular and distinctive output signals over all concentrations used in this work. These results are related to the synergism between the compounds on the active layer, leading to a surface morphology that allowed fast analyte diffusion owing to an adequate molecular accommodation, which also preserved the urease activity. This work demonstrates the feasibility of employing LB films composed of lipids, CNT, algal polysaccharides, and enzymes as EIS devices for biosensing applications. Y1 - 2018 U6 - http://dx.doi.org/10.1021/acs.langmuir.7b04317 SN - 1520-5827 VL - 34 IS - 9 SP - 3082 EP - 3093 PB - ACS Publications CY - Washington, DC ER - TY - BOOK A1 - Schöning, Michael Josef A1 - Poghossian, Arshak T1 - Label-free biosensing: advanced materials, devices and applications Y1 - 2018 SN - 978-3-319-75219-8 PB - Springer CY - Cham ER - TY - CHAP A1 - Yoshinobu, Tatsuo A1 - Krause, Steffi A1 - Miyamoto, Ko-ichiro A1 - Werner, Frederik A1 - Poghossian, Arshak A1 - Wagner, Torsten A1 - Schöning, Michael Josef T1 - (Bio-)chemical Sensing and Imaging by LAPS and SPIM T2 - Label-free biosensing: advanced materials, devices and applications N2 - The light-addressable potentiometric sensor (LAPS) and scanning photo-induced impedance microscopy (SPIM) are two closely related methods to visualise the distributions of chemical species and impedance, respectively, at the interface between the sensing surface and the sample solution. They both have the same field-effect structure based on a semiconductor, which allows spatially resolved and label-free measurement of chemical species and impedance in the form of a photocurrent signal generated by a scanning light beam. In this article, the principles and various operation modes of LAPS and SPIM, functionalisation of the sensing surface for measuring various species, LAPS-based chemical imaging and high-resolution sensors based on silicon-on-sapphire substrates are described and discussed, focusing on their technical details and prospective applications. KW - Chemical imaging KW - Field-effect device KW - Light-addressable potentiometric sensor KW - Potentiometry Y1 - 2018 SN - 978-3-319-75219-8 SP - 103 EP - 132 PB - Springer CY - Cham ER - TY - CHAP A1 - Bung, Daniel B. A1 - Tullis, Blake T1 - Hydraulic Structures - ISHS2018 in Perspective T2 - 7th IAHR International Symposium on Hydraulic Structures, Aachen, Germany, 15-18 May Y1 - 2018 SN - 978-0-692-13277-7 U6 - http://dx.doi.org/10.15142/T3WH2B ER - TY - CHAP A1 - Valero, Daniel A1 - Vogel, Jochen A1 - Schmidt, Daniel A1 - Bung, Daniel B. T1 - Three-dimensional flow structure inside the cavity of a non-aerated stepped chute T2 - 7th IAHR International Symposium on Hydraulic Structures, Aachen, Germany, 15-18 May Y1 - 2018 SN - 978-0-692-13277-7 U6 - http://dx.doi.org/10.15142/T3GH17 ER - TY - CHAP A1 - Jenert, Tobias A1 - Barnat, Miriam A1 - Dilger, Bernadette T1 - Struktur, Prozess oder Didaktik als Ausgangspunkt? - Ein integratives Modell der Curriculumentwicklung an Hochschule T2 - Gelingende Lehre: erkennen, entwickeln, etablieren : Beiträge der Jahrestagung der Deutschen Gesellschaft für Hochschuldidaktik (dghd) 2016 Y1 - 2018 SN - 9783763959419 N1 - Blickpunkt Hochschuldidaktik, Vol. 132 SP - 149 EP - 164 PB - wbv Media CY - Bielefeld ER - TY - CHAP A1 - Ludowicy, Jonas A1 - Rings, René A1 - Finger, Felix A1 - Braun, Carsten T1 - Sizing Studies of Light Aircraft with Serial Hybrid Propulsion Systems T2 - Luft- und Raumfahrt - Digitalisierung und Vernetzung : Deutscher Luft- und Raumfahrtkongress 2018. 4. - 6. September 2018 - Friedrichshafen Y1 - 2018 ER - TY - CHAP A1 - Finger, Felix A1 - Götten, Falk A1 - Braun, Carsten T1 - Initial Sizing for a Family of Hybrid-Electric VTOL General Aviation Aircraft T2 - 67. Deutscher Luft- und Raumfahrtkongress 2018 Y1 - 2018 ER - TY - CHAP A1 - Ludowicy, Jonas A1 - Rings, René A1 - Finger, Felix A1 - Braun, Carsten T1 - Sizing Studies of Light Aircraft with Parallel Hybrid Propulsion Systems T2 - Deutscher Luft- und Raumfahrtkongress 2018 Y1 - 2018 U6 - http://dx.doi.org/10.25967/480227 ER - TY - JOUR A1 - Götten, Falk A1 - Finger, Felix A1 - Havermann, Marc A1 - Braun, Carsten A1 - Gomez, Francisco A1 - Bill, C. T1 - On the flight performance impact of landing gear drag reduction methods for unmanned air vehicles JF - Deutscher Luft- und Raumfahrtkongress 2018 N2 - The flight performance impact of three different landing gear configurations on a small, fixed-wing UAV is analyzed with a combination of RANS CFD calculations and an incremental flight performance algorithm. A standard fixed landing gear configuration is taken as a baseline, while the influence of retracting the landing gear or applying streamlined fairings is investigated. A retraction leads to a significant parasite drag reduction, while also fairings promise large savings. The increase in lift-to-drag ratio is reduced at high lift coefficients due to the influence of induced drag. All configurations are tested on three different design missions with an incremental flight performance algorithm. A trade-off study is performed using the retracted or faired landing gear's weight increase as a variable. The analysis reveals only small mission performance gains as the aerodynamic improvements are negated by weight penalties. A new workflow for decision-making is presented that allows to estimate if a change in landing gear configuration is beneficial for a small UAV. Y1 - 2018 U6 - http://dx.doi.org/10.25967/480058 PB - DGLR CY - Bonn ER - TY - THES A1 - Keinz, Jan T1 - Optimization of a Dry Low NOx Micromix Combustor for an Industrial Gas Turbine Using Hydrogen-Rich Syngas Fuel Y1 - 2018 N1 - Dissertation submitted for the degree of Doctor of Engineering Sciences and Technology ; in Cooperation with Aachen university of Applied Sciences, Department Aerospace Technology; Thesis director: Prof. P. Hendrick; Thesis co-director: Prof. H. Funke PB - Université Libre de Bruxelles - Brussels School of Engineering Aero-Thermo-Mechanics CY - Brüssel ER - TY - CHAP A1 - Richter, Charlotte A1 - Braunstein, Bjoern A1 - Stäudle, Benjamin A1 - Attias, Julia A1 - Suess, Alexander A1 - Weber, T. A1 - Rittweger, Joern A1 - Green, David A. A1 - Albracht, Kirsten T1 - In vivo fascicle length of the gastrocnemius muscle during walking in simulated martian gravity using two different body weight support devices T2 - 23rd Annual Congress of the European College of Sport Science, Dublin, Irland Y1 - 2018 ER - TY - JOUR A1 - Bernecker, Andreas A1 - Klier, Julia A1 - Stern, Sebastian A1 - Thiel, Lea T1 - Sustaining high performance beyond public-sector pilot projects. Y1 - 2018 IS - September 2018 ER -