TY - JOUR A1 - Ribitsch, Doris A1 - Karl, Wolfgang A1 - Birner-Gruenberger, Ruth A1 - Gruber, Karl A1 - Eiteljoerg, Inge A1 - Remler, Peter A1 - Wieland, Susanne A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schwab, Helmut T1 - C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli JF - Journal of biotechnology N2 - Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7–11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease. Y1 - 2010 U6 - https://doi.org/10.1016/j.jbiotec.2010.09.947 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 150 IS - 3 SP - 408 EP - 416 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Götten, Falk A1 - Finger, Felix A1 - Braun, Carsten A1 - Havermann, Marc A1 - Bil, Cees A1 - Gomez, Francisco T1 - Empirical Correlations for Geometry Build-Up of Fixed Wing Unmanned Air Vehicles T2 - APISAT 2018: The Proceedings of the 2018 Asia-Pacific International Symposium on Aerospace Technology (APISAT 2018) N2 - The results of a statistical investigation of 42 fixed-wing, small to medium sized (20 kg−1000 kg) reconnaissance unmanned air vehicles (UAVs) are presented. Regression analyses are used to identify correlations of the most relevant geometry dimensions with the UAV’s maximum take-off mass. The findings allow an empirical based geometry-build up for a complete unmanned aircraft by referring to its take-off mass only. This provides a bridge between very early design stages (initial sizing) and the later determination of shapes and dimensions. The correlations might be integrated into a UAV sizing environment and allow designers to implement more sophisticated drag and weight estimation methods in this process. Additional information on correlation factors for a rough drag estimation methodology indicate how this technique can significantly enhance the accuracy of early design iterations. KW - Unmanned Air Vehicle KW - Geometry KW - Correlations KW - Statistics KW - Drag Y1 - 2019 SN - 978-981-13-3305-7 U6 - https://doi.org/10.1007/978-981-13-3305-7_109 N1 - APISAT 2018 - Asia-Pacific International Symposium on Aerospace Technology. 16-18 October 2018. Chengdu, China. Lecture Notes in Electrical Engineering (LNEE, volume 459) SP - 1365 EP - 1381 PB - Springer CY - Singapore ER - TY - JOUR A1 - Ribitsch, Doris A1 - Heumann, Sonja A1 - Trotscha, Eva A1 - Herrero Acero, Enrique A1 - Greimel, Katrin A1 - Leber, Regina A1 - Birner-Gruenberger, Ruth A1 - Deller, Sigrid A1 - Eiteljoerg, Inge A1 - Remler, Peter A1 - Weber, Thomas A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Donelli, Ilaria A1 - Freddi, Giuliano A1 - Schwab, Helmut A1 - Guebitz, Georg M. T1 - Hydrolysis of polyethyleneterephthalate by p-nitrobenzylesterase from Bacillus subtilis JF - Biotechnology progress Y1 - 2011 SN - 1520-6033 (E-Journal); 8756-7938 (Print) VL - Vol. 27 IS - Iss. 4 SP - 951 EP - 960 PB - Wiley CY - Hoboken ER - TY - CHAP A1 - Uibel, Thomas A1 - Blaß, Hans Joachim T1 - Joints with Dowel Type Fasteners in CLT structures T2 - Focus solid timber solutions : European Conference on Cross Laminated Timber (CLT); May 21-22, 2013, Graz University of Technology, Austria Y1 - 2013 SN - 978-1-85790-181-8 ; 1-85790-181-9 SP - 119 EP - 134 ER - TY - JOUR A1 - Schaefer, Thomas A1 - Höfken, Hans-Wilhelm A1 - Schuba, Marko T1 - Windows Phone 7 from a Digital Forensics’ Perspective Y1 - 2011 U6 - https://doi.org/10.1007/978-3-642-35515-8_6 N1 - ICDF2C <3, 2011, Dublin> PB - Springer CY - Berlin ER - TY - CHAP A1 - Broenner, Simon A1 - Höfken, Hans-Wilhelm A1 - Schuba, Marko T1 - Streamlining extraction and analysis of android RAM images T2 - Proceedings of the 2nd International Conference on Information Systems Security and Privacy - ICISSP N2 - The Android operating system powers the majority of the world’s mobile devices and has been becoming increasingly important in day-to-day digital forensics. Therefore, technicians and analysts are in need of reliable methods for extracting and analyzing memory images from live Android systems. This paper takes different existing, extraction methods and derives a universal, reproducible, reliably documented method for both extraction and analysis. In addition the VOLIX II front-end for the Volatility Framework is extended with additional functionality to make the analysis of Android memory images easier for technically non-adept users. Y1 - 2016 SN - 978-989-758-167-0 U6 - https://doi.org/10.5220/0005652802550264 SP - 255 EP - 264 PB - SciTePress CY - Setúbal ER - TY - JOUR A1 - Schuba, Marko A1 - Reichl, Peter A1 - Kesdogan, Dogan A1 - Junghärtchen, Klaus T1 - Simulative Performance Evaluation of the Temporary Pseudonym Method for Protecting Location Information in GSM Networks / Reichl, P. ; Kesdogan, D. ; Junghärtchen, K. ; Schuba, M. JF - Computer Performance Evaluation - Modelling Techniques and Tools / Puigjaner, Ramon (eds.) Y1 - 1998 SN - 3-540-64949-2 N1 - Lecture notes in computer science ; 1469 SP - 105 EP - 116 PB - Springer CY - Berlin ER - TY - JOUR A1 - Schuba, Marko A1 - Haverkort, Boudewijn R. A1 - Schneider, Gaby T1 - Performance evaluation of multicast communication in packet-switched networks / Schuba, Marko ; Haverkort, Boudewijn R. ; Schneider, Gaby JF - Performance Evaluation. 39 (2000), H. 1-4 Y1 - 2000 SN - 0166-5316 U6 - https://doi.org/10.1016/S0166-5316(99)00058-9 SP - 61 EP - 80 ER - TY - JOUR A1 - Schuba, Marko A1 - Hermanns, Oliver T1 - Performance Investigations of the IP Multicast Architecture / Hermanns, Oliver ; Schuba, Marko JF - Performance of the IP Multicast Achitecture . Proceedings JENC 6. Proceedings of the 6th Joint European Networking Conference, Tel Aviv Y1 - 1995 N1 - Reprinted in Computer Networks and ISDN Systems 28 pp 429-439, 1996 SP - 121-1 EP - 121-8 ER - TY - JOUR A1 - Schuba, Marko A1 - Wrona, Konrad T1 - Security for Mobile Commerce Applications / Schuba, Marko ; Wrona, Konrad Y1 - 2001 N1 - Multimedia, Internet, Video Technologies 2001 (MIV 2001), Malta, September 1-6, 2001 ; WSEAS Conference ; World Scientific and Engineering Academy and Society SP - 1 EP - 8 ER - TY - JOUR A1 - Adels, Klaudia A1 - Thönnessen, Vera A1 - Monakhova, Yulia T1 - Complementary instrumental techniques applied to pain relieving tablets in an undergraduate laboratory experiment JF - Journal of Chemical Education N2 - Several unconnected laboratory experiments are usually offered for students in instrumental analysis lab. To give the students a more rational overview of the most common instrumental techniques, a new laboratory experiment was developed. Marketed pain relief drugs, familiar consumer products with one to three active components, namely, acetaminophen (paracetamol), acetylsalicylic acid (ASA), and caffeine, were selected. Common analytical methods were compared regarding the performance of qualitative and quantitative analysis of unknown tablets: UV–visible (UV–vis), infrared (IR), and nuclear magnetic resonance (NMR) spectroscopies, as well as high-performance liquid chromatography (HPLC). The students successfully uncovered the composition of formulations, which were divided into three difficulty categories. Students were shown that in addition to simple mixtures handled in theoretical classes, the composition of complex drug products can also be uncovered. By comparing the performance of different techniques, students deepen their understanding and compare the efficiency of analytical methods in the context of complex mixtures. The laboratory experiment can be adjusted for graduate level by including extra tasks such as method optimization, validation, and 2D spectroscopic techniques. KW - Instrumental analysis KW - Spectroscopy KW - Liquid chromatography KW - Qualitative and quantitative determination KW - Drugs Y1 - 2024 U6 - https://doi.org/10.1021/acs.jchemed.4c00681 SN - 0021-9584 (Print) SN - 1938-1328 (Online) PB - ACS ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Krause, Robin A1 - Block, Kirstin A1 - Musahmeh, Mustafa A1 - Mulchandani, Ashok A1 - Wang, Joseph T1 - A dual amperometric/potentiometric FIA-based biosensor for the distinctive detection of organophosphorus pesticides JF - Sensors and Actuators B. 95 (2003), H. 1-3 Y1 - 2003 SN - 0925-4005 SP - 291 EP - 296 ER - TY - JOUR A1 - Raue, Markus A1 - Wambach, M. A1 - Glöggler, Stefan A1 - Grefen, Dana A1 - Kaufmann, R. A1 - Abetz, C. A1 - Georgopanos, Prokopios A1 - Handge, U. A. A1 - Mang, Thomas A1 - Blümich, Bernhard A1 - Abetz, V. T1 - Investigation of historical hard rubber ornaments of Charles Goodyear JF - Macromolecular chemistry and physics Y1 - 2014 SN - 1022-1352 VL - Vol. 215 IS - No. 3 SP - 245 EP - 254 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Wang, J. A1 - Krause, R. A1 - Block, Kirstin A1 - Musameh, M. A1 - Mulchandani, A. A1 - Schöning, Michael Josef T1 - Flow injection amperometric detection of OP nerve agents based on organophosphorus-hydrolase biosensor detector JF - Biosensors & Bioelectronics. 18 (2003), H. 2-3 Y1 - 2003 SN - 0956-5663 SP - 255 EP - 260 ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Kloock, Joachim P. A1 - Knobbe, D.-T. A1 - Krause, R. A1 - Block, Kirstin A1 - Wang, J. A1 - Mulchandani, A. A1 - Keusgen, M. T1 - Direktnachweis von Pestiziden und Cyanid mit elektrochemischen Enzymsensoren JF - Sensoren und Messsysteme 2004 : Tagung Ludwigsburg, 15. und 16. März 2004 / VDI-VDE-Gesellschaft Mess- und Automatisierungstechnik Y1 - 2004 SN - 3-18-091829-2 N1 - Auch erschienen als: VDI-Berichte ; 1829 SP - 699 EP - 706 PB - VDI CY - Düsseldorf ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Krause, R. A1 - Block, Kirstin A1 - Musameh, M. A1 - Mulchandani, A. A1 - Wang, J. T1 - A dual amperometric / potentiometric FIA-based biosensor for the distinctive detection of organophosphorus pesticides JF - Book of abstracts / ed. by J. Saneistr. Y1 - 2002 SN - 80-01-02576-4 N1 - Eurosensors ; (16, 2002, Praha) SP - 76 EP - 79 PB - Czech Technical University, Faculty of Electrical Engineering, Department of Measurement CY - Prague ER - TY - JOUR A1 - Akimbekov, Nuraly A1 - Digel, Ilya A1 - Zhubanova, Azhar A1 - Tastambek, Kuanysh T. A1 - Tepecik, Atakan A1 - Sherelkhan, Dinara T1 - Biotechnological potentials of surfactants in coal utilization: a review JF - Environmental Science and Pollution Research N2 - The quest for scientifically advanced and sustainable solutions is driven by growing environmental and economic issues associated with coal mining, processing, and utilization. Consequently, within the coal industry, there is a growing recognition of the potential of microbial applications in fostering innovative technologies. Microbial-based coal solubilization, coal beneficiation, and coal dust suppression are green alternatives to traditional thermochemical and leaching technologies and better meet the need for ecologically sound and economically viable choices. Surfactant-mediated approaches have emerged as powerful tools for modeling, simulation, and optimization of coal-microbial systems and continue to gain prominence in clean coal fuel production, particularly in microbiological co-processing, conversion, and beneficiation. Surfactants (surface-active agents) are amphiphilic compounds that can reduce surface tension and enhance the solubility of hydrophobic molecules. A wide range of surfactant properties can be achieved by either directly influencing microbial growth factors, stimulants, and substrates or indirectly serving as frothers, collectors, and modifiers in the processing and utilization of coal. This review highlights the significant biotechnological potential of surfactants by providing a thorough overview of their involvement in coal biodegradation, bioprocessing, and biobeneficiation, acknowledging their importance as crucial steps in coal consumption. KW - Coal KW - Surfactants KW - Microorganisms KW - Biosolubilization KW - Biobeneficiation Y1 - 2024 U6 - https://doi.org/10.1007/s11356-024-34892-5 SN - 1614-7499 VL - 31 SP - 55099 EP - 55118 PB - Springer CY - Berlin ER - TY - JOUR A1 - Freyer, Nils A1 - Kempt, Hendrik A1 - Klöser, Lars T1 - Easy-read and large language models: on the ethical dimensions of LLM-based text simplification JF - Ethics and Information Technology N2 - The production of easy-read and plain language is a challenging task, requiring well-educated experts to write context-dependent simplifications of texts. Therefore, the domain of easy-read and plain language is currently restricted to the bare minimum of necessary information. Thus, even though there is a tendency to broaden the domain of easy-read and plain language, the inaccessibility of a significant amount of textual information excludes the target audience from partaking or entertainment and restricts their ability to live life autonomously. Large language models can solve a vast variety of natural language tasks, including the simplification of standard language texts to easy-read or plain language. Moreover, with the rise of generative models like GPT, easy-read and plain language may be applicable to all kinds of natural language texts, making formerly inaccessible information accessible to marginalized groups like, a.o., non-native speakers, and people with mental disabilities. In this paper, we argue for the feasibility of text simplification and generation in that context, outline the ethical dimensions, and discuss the implications for researchers in the field of ethics and computer science. KW - Large language models KW - Easy read KW - AI ethics KW - Natural language processing KW - Accessibility Y1 - 2024 U6 - https://doi.org/10.1007/s10676-024-09792-4 SN - 1388-1957 (Print) SN - 1572-8439 (Online) VL - 26 PB - Springer CY - Dortrecht ER - TY - JOUR A1 - Eichler, Fabian A1 - Balc, Nicolae A1 - Bremen, Sebastian A1 - Schleser, Markus A1 - Schwarz, Alexander T1 - Research on reducing residual stresses of SLM parts made for downstream welding process JF - Acta Technica Napocensis N2 - In the face of the current trend towards larger and more complex production tasks in the SLM process and the current limitations in terms of maximum build space, the welding of SLM components to each other or to conventionally manufactured parts is becoming increasingly relevant. The fusion welding of SLM components made of 316L has so far been rarely investigated and if so, then for highly specialised laser welding processes. When welding with industrial gas welding processes such as MIG/MAG or TIG welding, distortions occur which are associated with the resulting residual stresses in the components. This paper investigates process-side influencing factors to avoid resulting residual stresses in SLM components made of 316L. The aim is to develop a strategy to build up SLM components as stress-free as possible in order to join them as profitably as possible with a downstream welding process. For this purpose, influencing parameters such as laser power, scan speed, but also scan vector length and different scan patterns are investigated with regard to their influence on residual stresses. KW - Residual Stresses KW - Hybrid Manufacturing KW - Welding KW - SLM KW - LPBF KW - Additive Manufacturing Y1 - 2024 SN - 1221-5872 VL - 67 IS - 1s SP - 69 EP - 78 PB - Technical University of Cluj-Napoca CY - Cluj-Napoca ER - TY - JOUR A1 - Abbas, Karim A1 - Balc, Nicolae A1 - Bremen, Sebastian A1 - Hedwig, Lukas T1 - Polyetheretherketone (PEEK) in rapid tooling: advancements and applications for fused filament fabrication of rubber molds JF - Acta Technica Napocensis N2 - Establishing high-performance polymers in additive manufacturing opens up new industrial applications. Polyetheretherketone (PEEK) was initially used in aerospace but is now widely applied in automotive, electronics, and medical industries. This study focuses on developing applications using PEEK and Fused Filament Fabrication for cost-efficient vulcanization injection mold production. A proof of concept confirms PEEK’s suitability for AM mold making, withstanding vulcanization conditions. Printing PEEK above its glass transition temperature of 145 °C is preferable due to its narrow process window. A new process strategy at room temperature is discussed, with micrographs showing improved inter-layer bonding at 410°C nozzle temperature and 0.1 mm layer thickness. Minimizing the layer thickness from 0.15 mm to 0.1 mm improves tensile strength by 16%. KW - Process Parameters KW - Rapid Tooling KW - Polyetheretherketone (PEEK) KW - Tensile Strength KW - Fused Filament Fabrication KW - Additive Manufacturing Y1 - 2024 SN - 1221-5872 VL - 67 IS - 1s SP - 13 EP - 22 PB - Technical University of Cluj-Napoca CY - Cluj-Napoca ER -