TY  - JOUR
A1  - Unden, G.
A1  - Becker, S.
A1  - Bongaerts, Johannes
A1  - Schirawski, J.
A1  - Six, S.
T1  - Oxygen regulated gene expression in facultatively anaerobic bacteria
JF  - Antonie van Leeuwenhoek
Y1  - 1994
SN  - 0003-6072 (Print) ; 1572-9699 (online)
VL  - Vol. 66
IS  - Iss. 1-3
SP  - 3
EP  - 22
ER  - 
TY  - JOUR
A1  - Bongaerts, Johannes
A1  - Zoschke, Sascha
A1  - Weidner, Uwe
A1  - Linden, Gottfried
T1  - Transcriptional regulation of the proton translocating NADH
JF  - Molecular microbiology
Y1  - 1995
SN  - 1365-2958 (E-Journal); 0950-382x (Print)
VL  - Vol. 16
IS  - Iss. 3
SP  - 521
EP  - 534
ER  - 
TY  - JOUR
A1  - Unden, G.
A1  - Becker, S.
A1  - Bongaerts, Johannes
A1  - Holighaus, G.
A1  - Schirawski, J.
A1  - Six, S.
T1  - O2-sensing and O2-dependent gene regulation in facultatively anaerobic bacteria
JF  - Archives of microbiology
Y1  - 1995
SN  - 1432-072X (E-Journal); 0003-9276 (Print); 0302-8933 (Print)
VL  - Vol. 164
IS  - Iss. 2
SP  - 81
EP  - 90
ER  - 
TY  - JOUR
A1  - Wissenbach, U.
A1  - Six, S.
A1  - Bongaerts, Johannes
A1  - Ternes, D.
A1  - Steinwachs, S.
A1  - Unden, G.
T1  - A third periplasmic transport system for l-arginine in Escherichia coli: molecular characterization of the artPIQMJ genes, arginine binding and transport
JF  - Molecular microbiology
Y1  - 1995
SN  - 1365-2958 (E-Journal); 0950-382x (Print)
VL  - Vol. 17
IS  - Iss. 4
SP  - 675
EP  - 686
ER  - 
TY  - JOUR
A1  - Tran, Quang Hon
A1  - Bongaerts, Johannes
A1  - Vlad, Dorina
A1  - Unden, Gottfried
T1  - Requirement for the proton-pumping NADH dehydrogenase I of Escherichia coli in respiration of NADH to fumarate and its bioenergetic implications
JF  - European journal of biochemistry
Y1  - 1997
SN  - 0014-2956
VL  - Vol. 244
IS  - Iss. 1
SP  - 155
EP  - 160
ER  - 
TY  - JOUR
A1  - Unden, Gottfried
A1  - Bongaerts, Johannes
T1  - Alternative respiratory pathways of Escherichia coli: energetics and transcriptional regulation in response to electron acceptors
JF  - Biochimica et biophysica acta (BBA) - Bioenergetics
Y1  - 1997
SN  - 1879-2650 (E-Journal); 0005-2728 (Print)
VL  - Vol. 1320
IS  - Iss. 3
SP  - 217
EP  - 234
ER  - 
TY  - JOUR
A1  - Zientz, Evelyn
A1  - Bongaerts, Johannes
A1  - Unden, Gottfried
T1  - Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system
JF  - Journal of bacteriology
Y1  - 1998
SN  - 1098-5530 (E-Journal); 0021-9193 (Print)
VL  - Vol. 180
IS  - No. 20
SP  - 5421
EP  - 5425
ER  - 
TY  - JOUR
A1  - Wackwitz, B.
A1  - Bongaerts, Johannes
A1  - Goodman, S. D.
A1  - Unden, Gottfried
T1  - Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significance
JF  - Molecular and general genetics : MGG
Y1  - 1999
SN  - 1617-4623 (E-Journal); 1617-4615 (Print)
VL  - Vol. 262
IS  - Iss. 4 - 5
SP  - 876
EP  - 883
ER  - 
TY  - JOUR
A1  - Müller, Ulrike
A1  - Bongaerts, Johannes
A1  - Bovenberg, Roel
A1  - Jossek, Ralf
A1  - Krämer, Marco
A1  - Linnemann, J.
A1  - Müschen, S.
A1  - Ritterbecks, S.
A1  - Sprenger, G.
A1  - Wubbolts, Marcel
T1  - Metabolic engineering to produce fine chemicals in Escherichia coli
JF  - Mededelingen van de Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent
Y1  - 2001
SN  - 0035-533x
VL  - 66 (3a)
SP  - 215
EP  - 217
ER  - 
TY  - JOUR
A1  - Jossek, Ralf
A1  - Bongaerts, Johannes
A1  - Sprenger, Georg A.
T1  - Characterization of a new feedback-resistant 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase AroF of Escherichia coli
JF  - FEMS microbiology letters
Y1  - 2001
SN  - 1574-6968
VL  - Vol. 202
IS  - Iss. 1
SP  - 145
EP  - 148
ER  - 
TY  - JOUR
A1  - Bongaerts, Johannes
A1  - Krämer, Marco
A1  - Müller, Ulrike
A1  - Raeven, Leon
A1  - Wubbolts, Marcel
T1  - Metabolic engineering for microbial production of aromatic amino acids and derived compounds
JF  - Metabolic engineering
Y1  - 2001
SN  - 1096-7184 (E-Journal); 1096-7176 (Print)
VL  - Vol. 3
IS  - Iss. 4
SP  - 289
EP  - 300
ER  - 
TY  - JOUR
A1  - Schmitz, M.
A1  - Hirsch, E.
A1  - Bongaerts, Johannes
A1  - Takors, Ralf
T1  - Pulse experiments as a prerequisite for the quantification of in vivo enzyme kinetics in aromatic amino acid pathway of Eschericia coli
JF  - Biotechnology progress
Y1  - 2002
SN  - 1520-6033 (E-Journal); 8756-7938 (Print)
VL  - Vol. 18
IS  - Iss. 5
SP  - 935
EP  - 941
ER  - 
TY  - JOUR
A1  - Bongaerts, Johannes
A1  - Bovenberg, Roel
A1  - Krämer, Marco
A1  - Müller, Ulrike
A1  - Raeven, Leon
A1  - Wubbolts, Marcel
T1  - Metabolic engineering to produce fine chemicals in Escherichia coli
JF  - Chemie - Ingenieur - Technik (CIT)
Y1  - 2002
SN  - 1522-2640 (E-Journal); 0009-286X (Print)
N1  - Printausg. in der Bibliothek vorhanden: 63 ZS 022
VL  - Vol. 74
IS  - Iss. 5
SP  - 694
ER  - 
TY  - JOUR
A1  - Gerigk, M.
A1  - Bujnicki, R.
A1  - Ganpo-Nkwenkwa, E.
A1  - Bongaerts, Johannes
A1  - Sprenger, G.
A1  - Takors, Ralf
T1  - Process control for enhanced L-phenylalanine production using different recombinant Escherichia coli strains
JF  - Biotechnology and bioengineering
Y1  - 2002
SN  - 1097-0290 (E-Journal); 0006-3592 (Print)
VL  - Vol. 80
IS  - Iss. 7
SP  - 746
EP  - 754
ER  - 
TY  - JOUR
A1  - Gerigk, M.
A1  - Maaß, D.
A1  - Kreutzer, A.
A1  - Sprenger, G.
A1  - Bongaerts, Johannes
A1  - Wubbolts, Marcel
A1  - Takors, Ralf
T1  - Enhanced pilot-scale fed-batch L-phenylalanine production with recombinant Escherichia coli by fully integrated reactive extraction
JF  - Bioprocess and biosystems engineering
Y1  - 2002
SN  - 1432-0797 (E-Journal); 1615-7605 (E-Journal); 0178-515X (Print); 1615-7591 (Print)
VL  - Vol. 25
IS  - Iss. 1
SP  - 43
EP  - 52
ER  - 
TY  - JOUR
A1  - Krämer, Marco
A1  - Bongaerts, Johannes
A1  - Bovenberg, Roel
A1  - Kremer, Susanne
A1  - Müller, Ulrike
A1  - Orf, Sonja
A1  - Wubbolts, Marcel
A1  - Raeven, Leon
T1  - Metabolic engineering for microbial production of shikimic acid
JF  - Metabolic engineering
Y1  - 2003
SN  - 1096-7184 (E-Journal); 1096-7176 (Print)
VL  - Vol. 5
IS  - Iss. 4
SP  - 277
EP  - 283
ER  - 
TY  - JOUR
A1  - Polen, T.
A1  - Krämer, Marco
A1  - Bongaerts, Johannes
A1  - Wubbolts, Marcel
A1  - Wendisch, V. F.
T1  - The global gene expression response of Escherichia coli to L-phenylalanine
JF  - Journal of biotechnology
Y1  - 2005
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - Vol. 115
IS  - Iss. 3
SP  - 221
EP  - 237
ER  - 
TY  - JOUR
A1  - Degering, Christian
A1  - Eggert, Thorsten
A1  - Puls, Michael
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Maurer, Karl-Heinz
A1  - Jaeger, Karl-Erich
T1  - Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides
JF  - Applied and environmental microbiology
N2  - Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains.
Y1  - 2010
U6  - http://dx.doi.org/10.1128/AEM.01146-10
SN  - 1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)
VL  - 76
IS  - 19
SP  - 6370
EP  - 6378
PB  - American Society for Microbiology
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Deppe, Veronika Maria
A1  - Bongaerts, Johannes
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
A1  - Meinhardt, Friedhelm
T1  - Enzymatic deglycation of Amadori products in bacteria
JF  - Applied microbiology and biotechnology
Y1  - 2011
SN  - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)
VL  - Vol. 90
IS  - Iss. 2
SP  - 399
EP  - 406
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Deppe, Veronika Maria
A1  - Klatte, Stephanie
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - O'Connell, Timothy
A1  - Meinhardt, Friedhelm
T1  - Genetic control of Amadori product degradation in Bacillus subtilis via regulation of frlBONMD expression by FrlR
JF  - Applied and environmental microbiology
Y1  - 2011
SN  - 1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)
VL  - Vol. 77
IS  - No. 9
SP  - 2839
EP  - 2846
PB  - American Society of Mechanical Engineers (ASME)
CY  - New York
ER  -