TY  - JOUR
A1  - Dantism, Shahriar
A1  - Takenaga, Shoko
A1  - Wagner, Torsten
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Differential imaging of the metabolism of bacteria and eukaryotic cells based on light-addressable potentiometric sensors
JF  - Electrochimica Acta
N2  - A light-addressable potentiometric sensor (LAPS) is a field-effect-based potentiometric sensor with an electrolyte/insulator/semiconductor (EIS) structure, which is able to monitor analyte concentrations of (bio-)chemical species in aqueous solutions in a spatially resolved way. Therefore, it is also an appropriate tool to record 2D-chemical images of concentration variations on the sensor surface. In the present work, two differential, LAPS-based measurement principles are introduced to determine the metabolic activity of Escherichia coli (E. coli) K12 and Chinese hamster ovary (CHO) cells as test microorganisms. Hereby, we focus on i) the determination of the extracellular acidification rate (ΔpH/min) after adding glucose solutions to the cell suspensions; and ii) recording the amplitude increase of the photocurrent (Iph) related to the produced acids from E. coli K12 bacteria and CHO cells on the sensor surface by 2D-chemical imaging. For this purpose, 3D-printed multi-chamber structures were developed and mounted on the planar sensor-chip surface to define four independent compartments, enabling differential measurements with varying cell concentrations. The differential concept allows eliminating unwanted drift effects and, with the four-chamber structures, measurements on the different cell concentrations were performed simultaneously, thus reducing also the overall measuring time.
Y1  - 2017
U6  - http://dx.doi.org/10.1016/j.electacta.2017.05.196
SN  - 0013-4686
VL  - 246
SP  - 234
EP  - 241
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Dikta, Gerhard
T1  - Semi-parametric random censorship models
JF  - From Statistics to Mathematical Finance : Festschrift in Honour of Winfried Stute
Y1  - 2017
SN  - 978-3-319-50986-0
U6  - http://dx.doi.org/10.1007/978-3-319-50986-0_3
SP  - 43
EP  - 56
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Engelmann, Ulrich M.
A1  - Buhl, Eva Miriam
A1  - Baumann, Martin
A1  - Schmitz-Rode, Thomas
A1  - Slabu, Ioana
T1  - Agglomeration of magnetic nanoparticles and its effects on magnetic hyperthermia
JF  - Current Directions in Biomedical Engineering
Y1  - 2017
U6  - http://dx.doi.org/10.1515/cdbme-2017-0096
SN  - 2364-5504
VL  - 3
IS  - 2
SP  - 457
EP  - 460
PB  - De Gruyter
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Gamella, Maria
A1  - Zakharchenko, Andrey
A1  - Guz, Nataliia
A1  - Masi, Madeline
A1  - Minko, Sergiy
A1  - Kolpashchikov, Dmitry M.
A1  - Iken, Heiko
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
A1  - Katz, Evgeny
T1  - DNA computing system activated by electrochemically triggered DNA realease from a polymer-brush-modified electrode array
JF  - Electroanalysis
N2  - An array of four independently wired indium tin oxide (ITO) electrodes was used for electrochemically stimulated DNA release and activation of DNA-based Identity, AND and XOR logic gates. Single-stranded DNA molecules were loaded on the mixed poly(N,N-dimethylaminoethyl methacrylate) (PDMAEMA)/poly(methacrylic acid) (PMAA) brush covalently attached to the ITO electrodes. The DNA deposition was performed at pH 5.0 when the polymer brush is positively charged due to protonation of tertiary amino groups in PDMAEMA, thus resulting in electrostatic attraction of the negatively charged DNA. By applying electrolysis at −1.0 V(vs. Ag/AgCl reference) electrochemical oxygen reduction resulted in the consumption of hydrogen ions and local pH increase near the electrode surface. The process resulted in recharging the polymer brush to the negative state due to dissociation of carboxylic groups of PMAA, thus repulsing the negatively charged DNA and releasing it from the electrode surface. The DNA release was performed in various combinations from different electrodes in the array assembly. The released DNA operated as input signals for activation of the Boolean logic gates. The developed system represents a step forward in DNA computing, combining for the first time DNA chemical processes with electronic input signals.
Y1  - 2017
U6  - http://dx.doi.org/10.1002/elan.201600389
SN  - 1521-4109
VL  - 29
IS  - 2
SP  - 398
EP  - 408
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Garibaldi, F.
A1  - Beging, Stefan
A1  - Canese, R.
A1  - Carpinelli, G.
A1  - Clinthorne, N.
A1  - Colilli, S.
A1  - Cosentino, L.
A1  - Finocchiaro, P.
A1  - Giuliani, F.
A1  - Gricia, M.
A1  - Lucentini, M.
A1  - Majewski, S.
A1  - Monno, E.
A1  - Musico, P.
A1  - Santavenere, F.
A1  - Tödter, J.
A1  - Wegener, Hans-Peter
A1  - Ziemons, Karl
T1  - A novel TOF-PET MRI detector for diagnosis and follow up of the prostate cancer
JF  - European Physical Journal Plus
Y1  - 2017
U6  - http://dx.doi.org/10.1140/epjp/i2017-11662-x
SN  - 2190-5444
VL  - 132
IS  - 9
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Hackl, M.
A1  - Andermahr, J.
A1  - Staat, Manfred
A1  - Bremer, I.
A1  - Borggrefe, J.
A1  - Prescher, A.
A1  - Müller, L. P.
A1  - Wegmann, K.
T1  - Suture button reconstruction of the central band of the interosseous membrane in Essex-Lopresti lesions: a comparative biomechanical investigation
JF  - The Journal of Hand Surgery (European Volume)
Y1  - 2017
U6  - http://dx.doi.org/10.1177/1753193416665943
SN  - 2043-6289 (Online)
SN  - 1753-1934 (Print)
VL  - 42
IS  - 4
SP  - 370
EP  - 376
PB  - Sage
CY  - London
ER  - 
TY  - JOUR
A1  - Hackl, Michael
A1  - Wegmann, Kilian
A1  - Kahmann, Stephanie Lucina
A1  - Heinze, Nicolai
A1  - Staat, Manfred
A1  - Neiss, Wolfram F.
A1  - Scaal, Martin
A1  - Müller, Lars P.
T1  - Radial shortening osteotomy reduces radiocapitellar contact pressures while preserving valgus stability of the elbow
JF  - Knee Surgery, Sports Traumatology, Arthroscopy
Y1  - 2017
U6  - http://dx.doi.org/10.1007/s00167-017-4468-z
SN  - 1433-7347
VL  - 25
IS  - 7
SP  - 2280
EP  - 2288
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Honarvarfard, Elham
A1  - Gamella, Maria
A1  - Channaveerappa, Devika
A1  - Darie, Costel C.
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
A1  - Katz, Evgeny
T1  - Electrochemically Stimulated Insulin Release from a Modified Graphene–functionalized Carbon Fiber Electrode
JF  - Electroanalysis
N2  - A graphene-functionalized carbon fiber electrode was modified with adsorbed polyethylenimine to introduce amino functionalities and then with trigonelline and 4-carboxyphenylboronic acid covalently bound to the amino groups. The trigonelline species containing quarterized pyridine groups produced positive charge on the electrode surface regardless of the pH value, while the phenylboronic acid species were neutral below pH 8 and negatively charged above pH 9 (note that their pKa=8.4). The total charge on the monolayer-modified electrode was positive at the neutral pH and negative at pH > 9. Note that 4-carboxyphenylboronic acid was attached to the electrode surface in molar excess to trigonelline, thus allowing the negative charge to dominate on the electrode surface at basic pH. Negatively charged fluorescent dye-labeled insulin (insulin-FITC) was loaded on the modified electrode surface at pH 7.0 due to its electrostatic attraction to the positively charged interface. The local pH in close vicinity to the electrode surface was increased to ca. 9–10 due to consumption of H+ ions upon electrochemical reduction of oxygen proceeding at the potential of −1.0 V (vs. Ag/AgCl) applied on the modified electrode. The process resulted in recharging of the electrode surface to the negative value due to the formation of the negative charge on the phenylboronic acid groups, thus resulting in the electrostatic repulsion of insulin-FITC and stimulating its release from the electrode surface. The insulin release was characterized by fluorescence spectroscopy (using the FITC-labeled insulin), by electrochemical measurements on an iridium oxide, IrOx, electrode and by mass spectrometry. The graphene-functionalized carbon fiber electrode demonstrated significant advantages in the signal-stimulated insulin release comparing with the carbon fiber electrode without the graphene species.
Y1  - 2017
U6  - http://dx.doi.org/10.1002/elan.201700095
SN  - 1521-4109
VL  - 29
IS  - 6
SP  - 1543
EP  - 1553
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Honarvarfard, Elham
A1  - Gamella, Maria
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
A1  - Katz, Evgeny
T1  - An enzyme-based reversible Controlled NOT (CNOT) logic gate operating on a semiconductor transducer
JF  - Applied Materials Today
N2  - An enzyme-based biocatalytic system mimicking operation of a logically reversible Controlled NOT (CNOT) gate has been interfaced with semiconductor electronic transducers. Electrolyte–insulator–semiconductor (EIS) structures have been used to transduce chemical changes produced by the enzyme system to an electronically readable capacitive output signal using field-effect features of the EIS device. Two enzymes, urease and esterase, were immobilized on the insulating interface of EIS structure producing local pH changes performing XOR logic operation controlled by various combinations of the input signals represented by urea and ethyl butyrate. Another EIS transducer was functionalized with esterase only, thus performing Identity (ID) logic operation for the ethyl butyrate input. Both semiconductor devices assembled in parallel operated as a logically reversible CNOT gate. The present system, despite its simplicity, demonstrated for the first time logically reversible function of the enzyme system transduced electronically with the semiconductor devices. The biomolecular realization of a CNOT gate interfaced with semiconductors is promising for integration into complex biomolecular networks and future biosensor/biomedical applications.
KW  - Electrolyte–insulator–semiconductor
KW  - Capacitive field-effect
KW  - CNOT
KW  - XOR
KW  - Enzyme logic gate
Y1  - 2017
U6  - http://dx.doi.org/10.1016/j.apmt.2017.08.003
SN  - 2352-9407
VL  - 9
SP  - 266
EP  - 270
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Horbach, Andreas
A1  - Duong, Minh Tuan
A1  - Staat, Manfred
T1  - Modelling of compressible and orthotropic surgical mesh implants based on optical deformation measurement
JF  - Journal of the mechanical behavior of biomedical materials
Y1  - 2017
U6  - http://dx.doi.org/10.1016/j.jmbbm.2017.06.012
SN  - 1751-6161
VL  - 74
SP  - 400
EP  - 410
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Jildeh, Zaid B.
A1  - Kirchner, Patrick
A1  - Oberländer, Jan
A1  - Kremers, Alexander
A1  - Wagner, Torsten
A1  - Wagner, Patrick H.
A1  - Schöning, Michael Josef
T1  - FEM-based modeling of a calorimetric gas sensor for hydrogen peroxide monitoring
JF  - physica status solidi a : applications and materials sciences
N2  - A physically coupled finite element method (FEM) model is developed to study the response behavior of a calorimetric gas sensor. The modeled sensor serves as a monitoring device of the concentration of gaseous hydrogen peroxide (H2 O2) in a high temperature mixture stream in aseptic sterilization processes. The principle of operation of a calorimetric H2 O2 sensor is analyzed and the results of the numerical model have been validated by using previously published sensor experiments. The deviation in the results between the FEM model and experimental data are presented and discussed.
Y1  - 2017
U6  - http://dx.doi.org/10.1002/pssa.201600912
SN  - 1862-6319
IS  - Early View
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Karnatak, Rajat
A1  - Kantz, Holger
A1  - Bialonski, Stephan
T1  - Early warning signal for interior crises in excitable systems
JF  - Physical Review E
Y1  - 2017
U6  - http://dx.doi.org/10.1103/PhysRevE.96.042211
SN  - 2470-0053
VL  - 96
IS  - 4
SP  - 042211
ER  - 
TY  - JOUR
A1  - Katz, Evgeny
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
T1  - Enzyme-based logic gates and circuits - analytical applications and interfacing with electronics
JF  - Analytical and Bioanalytical Chemistry
N2  - The paper is an overview of enzyme-based logic gates and their short circuits, with specific examples of Boolean AND and OR gates, and concatenated logic gates composed of multi-step enzyme-biocatalyzed reactions. Noise formation in the biocatalytic reactions and its decrease by adding a “filter” system, converting convex to sigmoid response function, are discussed. Despite the fact that the enzyme-based logic gates are primarily considered as components of future biomolecular computing systems, their biosensing applications are promising for immediate practical use. Analytical use of the enzyme logic systems in biomedical and forensic applications is discussed and exemplified with the logic analysis of biomarkers of various injuries, e.g., liver injury, and with analysis of biomarkers characteristic of different ethnicity found in blood samples on a crime scene. Interfacing of enzyme logic systems with modified electrodes and semiconductor devices is discussed, giving particular attention to the interfaces functionalized with signal-responsive materials. Future perspectives in the design of the biomolecular logic systems and their applications are discussed in the conclusion.
Y1  - 2017
U6  - http://dx.doi.org/10.1007/s00216-016-0079-7
SN  - 1618-2650
VL  - 409
SP  - 81
EP  - 94
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Kotliar, Konstantin
A1  - Hauser, Christine
A1  - Ortner, Marion
A1  - Muggenthaler, Claudia
A1  - Diehl-Schmid, Janine
A1  - Angermann, Susanne
A1  - Hapfelmeier, Alexander
A1  - Schmaderer, Christoph
A1  - Grimmer, Timo
T1  - Altered neurovascular coupling as measured by optical imaging: a biomarker for Alzheimer’s disease
JF  - Scientific Reports
Y1  - 2017
U6  - http://dx.doi.org/10.1038/s41598-017-13349-5
SN  - 2045-2322
N1  - Article 12906
VL  - 7
IS  - 1
SP  - 1
EP  - 11
PB  - Springer Nature
CY  - Cham
ER  - 
TY  - JOUR
A1  - Laack, Walter van
T1  - Neurophysiologisch ist das nicht alles zu erklären : Nahtoderfahrungen aus wissenschaftlicher Sicht
JF  - Der Allgemeinarzt
Y1  - 2017
SN  - 0172-7249
VL  - 38
IS  - 1
SP  - 4
EP  - 7
PB  - Universimed Deutschland GmbH
CY  - Mainz
ER  - 
TY  - JOUR
A1  - Lanzl, I.
A1  - Kotliar, Konstantin
T1  - Können Anti-VEGF-Injektionen Glaukom oder okuläre Hypertension verursachen?
T1  - Can Anti-VEGF Injections Cause Glaucoma or Ocular Hypertension?
JF  - Klinische Monatsblätter für Augenheilkunde
Y1  - 2017
U6  - http://dx.doi.org/10.1055/s-0043-101819
SN  - 0023-2165
VL  - 234
IS  - 2
SP  - 191
EP  - 193
PB  - Thieme
CY  - Stuttgart
ER  - 
TY  - JOUR
A1  - Michael, Hackl
A1  - Mayer, Katharina
A1  - Weber, Mareike
A1  - Staat, Manfred
A1  - van Riet, Roger
A1  - Burkhart, Klau Josef
A1  - Müller, Lars Peter
A1  - Wegmann, Kilian
T1  - Plate osteosynthesis of proximal ulna fractures : a biomechanical micromotion analysis
JF  - The journal of hand surgery
Y1  - 2017
U6  - http://dx.doi.org/10.1016/j.jhsa.2017.05.014
SN  - 0363-5023
VL  - 42
IS  - 10
SP  - 834.e1
EP  - 834.e7
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Miyamoto, Ko-ichiro
A1  - Hayashi, Kosuke
A1  - Sakamoto, Azuma
A1  - Werner, Frederik
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
A1  - Yoshinobu, Tatsuo
T1  - A high-Q resonance-mode measurement of EIS capacitive sensor by elimination of series resistance
JF  - Sensor and Actuators B: Chemical
N2  - An EIS capacitive sensor is a semiconductor-based potentiometric sensor, which is sensitive to the ion concentration or pH value of the solution in contact with the sensing surface. To detect a small change in the ion concentration or pH, a small capacitance change must be detected. Recently, a resonance-mode measurement was proposed, in which an inductor was connected to the EIS capacitive sensor and the resonant frequency was correlated with the pH value. In this study, the Q factor of the resonant circuit was enhanced by canceling the internal resistance of the reference electrode and the internal resistance of the inductor coil with the help of a bypass capacitor and a negative impedance converter, respectively. 1% variation of the signal in the developed system corresponded to a pH change of 3.93 mpH, which was about 1/12 of the conventional method, suggesting a better performance in detection of a small pH change.
KW  - Negative impedance convertor
KW  - Resonance-mode measurement
KW  - Chemical sensor
KW  - EIS capacitive sensor
Y1  - 2017
U6  - http://dx.doi.org/10.1016/j.snb.2017.03.002
SN  - 0925-4005
VL  - 248
SP  - 1006
EP  - 1010
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Molinnus, Denise
A1  - Poghossian, Arshak
A1  - Keusgen, Michael
A1  - Katz, Evgeny
A1  - Schöning, Michael Josef
T1  - Coupling of Biomolecular Logic Gates with Electronic Transducers: From Single Enzyme Logic Gates to Sense/Act/Treat Chips
JF  - Electroanalysis
N2  - The integration of biomolecular logic principles with electronic transducers allows designing novel digital biosensors with direct electrical output, logically triggered drug-release, and closed-loop sense/act/treat systems. This opens new opportunities for advanced personalized medicine in the context of theranostics. In the present work, we will discuss selected examples of recent developments in the field of interfacing enzyme logic gates with electrodes and semiconductor field-effect devices. Special attention is given to an enzyme OR/Reset logic gate based on a capacitive field-effect electrolyte-insulator-semiconductor sensor modified with a multi-enzyme membrane. Further examples are a digital adrenaline biosensor based on an AND logic gate with binary YES/NO output and an integrated closed-loop sense/act/treat system comprising an amperometric glucose sensor, a hydrogel actuator, and an insulin (drug) sensor.
Y1  - 2017
U6  - http://dx.doi.org/10.1002/elan.201700208
SN  - 1521-4109
VL  - 29
IS  - 8
SP  - 1840
EP  - 1849
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Morais, Paulo V.
A1  - Gomes, Vanderley F., Jr.
A1  - Silva, Anielle C. A.
A1  - Dantas, Noelio O.
A1  - Schöning, Michael Josef
A1  - Siqueira, José R., Jr.
T1  - Nanofilm of ZnO nanocrystals/carbon nanotubes as biocompatible layer for enzymatic biosensors in capacitive field-effect devices
JF  - Journal of Materials Science
N2  - The incorporation of nanomaterials that are biocompatible with different types of biological compounds has allowed the development of a new generation of biosensors applied especially in the biomedical field. In particular, the integration of film-based nanomaterials employed in field-effect devices can be interesting to develop biosensors with enhanced properties. In this paper, we studied the fabrication of sensitive nanofilms combining ZnO nanocrystals and carbon nanotubes (CNTs), prepared by means of the layer-by-layer (LbL) technique, in a capacitive electrolyte-insulator-semiconductor (EIS) structure for detecting glucose and urea. The ZnO nanocrystals were incorporated in a polymeric matrix of poly(allylamine) hydrochloride (PAH), and arranged with multi-walled CNTs in a LbL PAH-ZnO/CNTs film architecture onto EIS chips. The electrochemical characterizations were performed by capacitance–voltage and constant capacitance measurements, while the morphology of the films was characterized by atomic force microscopy. The enzymes glucose oxidase and urease were immobilized on film’s surface for detection of glucose and urea, respectively. In order to obtain glucose and urea biosensors with optimized amount of sensitive films, we investigated the ideal number of bilayers for each detection system. The glucose biosensor showed better sensitivity and output signal for an LbL PAH-ZnO/CNTs nanofilm with 10 bilayers. On the other hand, the urea biosensor presented enhanced properties even for the first bilayer, exhibiting high sensitivity and output signal. The presence of the LbL PAH-ZnO/CNTs films led to biosensors with better sensitivity and enhanced response signal, demonstrating that the adequate use of nanostructured films is feasible for proof-of-concept biosensors with improved properties that may be employed for biomedical applications.
Y1  - 2017
U6  - http://dx.doi.org/10.1007/s10853-017-1369-y
SN  - 1573-4803
VL  - 52
IS  - 20
SP  - 12314
EP  - 12325
PB  - Springer
CY  - Berlin
ER  -