TY  - JOUR
A1  - Orzada, S.
A1  - Maderwald, S.
A1  - Poser, B. A.
A1  - Johst, S.
A1  - Kannengiesser, S.
A1  - Ladd, M. E.
A1  - Bitz, Andreas
T1  - Time-interleaved acquisition of modes: an analysis of SAR and image contrast implications
JF  - Magnetic Resonance in Medicine
N2  - s the magnetic field strength and therefore the operational frequency in MRI are increased, the radiofrequency wavelength approaches the size of the human head/body, resulting in wave effects which cause signal decreases and dropouts. Especially, whole-body imaging at 7 T and higher is therefore challenging. Recently, an acquisition scheme called time-interleaved acquisition of modes has been proposed to tackle the inhomogeneity problems in high-field MRI. The basic premise is to excite two (or more) different Burn:x-wiley:07403194:media:MRM23081:tex2gif-stack-1 modes using static radiofrequency shimming in an interleaved acquisition, where the complementary radiofrequency patterns of the two modes can be exploited to improve overall signal homogeneity. In this work, the impact of time-interleaved acquisition of mode on image contrast as well as on time-averaged specific absorption rate is addressed in detail. Time-interleaved acquisition of mode is superior in Burn:x-wiley:07403194:media:MRM23081:tex2gif-stack-2 homogeneity compared with conventional radiofrequency shimming while being highly specific absorption rate efficient. Time-interleaved acquisition of modes can enable almost homogeneous high-field imaging throughout the entire field of view in PD, T2, and T2*-weighted imaging and, if a specified homogeneity criterion is met, in T1-weighted imaging as well.
Y1  - 2012
U6  - https://doi.org/10.1002/mrm.23081
SN  - 1522-2594
VL  - 67
IS  - 4
SP  - 1033
EP  - 1041
PB  - Wiley-Liss
CY  - New York
ER  - 
TY  - JOUR
A1  - Paulßen, Elisabeth
A1  - Kong, Shushu
A1  - Arciszewski, Pawel
A1  - Wielbalck, Swantje
A1  - Abram, Ulrich
T1  - Aryl and NHC Compounds of Technetium and Rhenium
JF  - Journal of the American Chemical Society
N2  - Air- and water-stable phenyl complexes with nitridotechnetium(V) cores can be prepared by straightforward procedures. [TcNPh2(PPh3)2] is formed by the reaction of [TcNCl2(PPh3)2] with PhLi. The analogous N-heterocyclic carbene (NHC) compound [TcNPh2(HLPh)2], where HLPh is 1,3,4-triphenyl-1,2,4-triazol-5-ylidene, is available from (NBu4)[TcNCl4] and HLPh or its methoxo-protected form. The latter compound allows the comparison of different Tc–C bonds within one compound. Surprisingly, the Tc chemistry with such NHCs does not resemble that of corresponding Re complexes, where CH activation and orthometalation dominate.
Y1  - 2012
U6  - https://doi.org/10.1021/ja3033718
SN  - 1520-5126
VL  - 134
IS  - 22
SP  - 9118
EP  - 9121
PB  - ACS Publications
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Paulßen, Elisabeth
A1  - Ngyugen, Hung Huy
A1  - Kahlcke, Nils
A1  - Deflon, Victor M.
A1  - Abram, Ulrich
T1  - Tricarbonyltechnetium(I) and -rhenium(I) complexes with N′-thiocarbamoylpicolylbenzamidines
JF  - Polyhedron
N2  - N,N-Dialkylamino(thiocarbonyl)-N′-picolylbenzamidines react with (NEt4)2[M(CO)3X3] (M = Re, X = Br; M = Tc, X = Cl) under formation of neutral [M(CO)3L] complexes in high yields. The monoanionic NNS ligands bind in a facial coordination mode and can readily be modified at the (CS)NR1R2 moiety. The complexes [99Tc(CO)3(LPyMor)] and [Re(CO)3(L)] (L = LPyMor, LPyEt) were characterized by X-ray diffraction. Reactions of [99mTc(CO)3(H2O)3]+ with the N′-thiocarbamoylpicolylbenzamidines give the corresponding 99mTc complexes. The ester group in HLPyCOOEt allows linkage between biomolecules and the metal core.
Y1  - 2012
U6  - https://doi.org/10.1016/j.poly.2012.04.008
SN  - 0277-5387
VL  - 40
IS  - 1
SP  - 153
EP  - 158
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Pieper, Martin
A1  - Klein, Peter
T1  - Application of simple, periodic homogenization techniques to non-linear heat conduction problems in non-periodic, porous media
JF  - Heat mass transfer
N2  - Often, detailed simulations of heat conduction in complicated, porous media have large runtimes. Then homogenization is a powerful tool to speed up the calculations by preserving accurate solutions at the same time. Unfortunately real structures are generally non-periodic, which requires unpractical, complicated homogenization techniques. We demonstrate in this paper, that the application of simple, periodic techniques to realistic media, that are just close to periodic, gives accurate, approximative solutions. In order to obtain effective parameters for the homogenized heat equation, we have to solve a so called “cell problem”. In contrast to periodic structures it is not trivial to determine a suitable unit cell, which represents a non-periodic media. To overcome this problem, we give a rule of thumb on how to choose a good cell. Finally we demonstrate the efficiency of our method for virtually generated foams as well as real foams and compare these results to periodic structures.
Y1  - 2012
U6  - https://doi.org/10.1007/s00231-011-0879-4
SN  - 0947-7411
VL  - 48
IS  - 2
SP  - 291
EP  - 300
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Weil, M. H.
A1  - Bäcker, Matthias
A1  - Mayer, D.
A1  - Schöning, Michael Josef
T1  - Field-effect Devices Functionalised with Gold-Nanoparticle/Macromolecule Hybrids: New Opportunities for a Label-Free Biosensing
JF  - Procedia Engineering
N2  - Field-effect capacitive electrolyte-insulator-semiconductor (EIS) sensors functionalised with citrate-capped gold nanoparticles (AuNP) have been used for the electrostatic detection of macromolecules by their intrinsic molecular charge. The EIS sensor detects the charge changes in the AuNP/macromolecule hybrids induced by the adsorption or binding events. A feasibility of the proposed detection scheme has been exemplary demonstrated by realising EIS sensors for the detection of poly-D-lysine molecules.
Y1  - 2012
U6  - https://doi.org/10.1016/j.proeng.2012.09.136
SN  - 1877-7058
N1  - Part of special issue "26th European Conference on Solid-State Transducers, EUROSENSOR 2012"
IS  - 47
SP  - 273
EP  - 276
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Reisgen, Uwe
A1  - Olschok, Simon
A1  - Jakobs, Stefan
A1  - Schleser, Markus
A1  - Mokrov, Oleg
A1  - Rossiter, Eduardo
T1  - Laser beam submerged arc hybrid welding
JF  - Physics procedia
N2  - The laser beam-submerged arc hybrid welding method originates from the knowledge that, with increasing penetration depth, the laser beam process has a tendency to pore formation in the lower weld regions. The coupling with the energy-efficient submerged-arc process improves degassing and reduces the tendency to pore formation. The high deposition rate of the SA process in combination with the laser beam process offers, providing the appropriate choice of weld preparation, the possibility of welding plates with a thickness larger than 20° mm in a single pass, and also of welding thicker plates with the double-sided single pass technique.
Y1  - 2012
U6  - https://doi.org/10.1016/j.phpro.2012.10.016
SN  - 1875-3892
N1  - Part of special issue "Laser assisted net shape engineering 7 (LANE 2012)"
VL  - 39
SP  - 75
EP  - 83
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Reisgen, Uwe
A1  - Schleser, Markus
A1  - Abdurakhmanov, Aydemir
A1  - Gumenyuk, Andrey
T1  - Measuring of plasma properties induced by non-vacuum electron beam welding
JF  - Physics of plasma
N2  - Electron beam plasma measurement was realised by means of DIABEAM system invented by ISF RWTH Aachen. The Langmuir probe method is used for measurement. The relative simplicity of the method and the possibility of dispersion of high power on the probe allow its application for the investigation of high-power electron beams. The key element of the method is a rotating thin tungsten wire, which intersects the beam transversely on its axis and collects part of the current by itself. The signals, which are registered in the DIABEAM as a voltage, were taken in the form of amplitude. The conversion of the probe current into the distribution along the beam radius was realised using the Abel’s method. A voltage-current characteristic was built for the beam current. The local electron density as well as the electron temperature, the floating potential and the plasma potential were measured and calculated by means of this characteristic.
Y1  - 2012
U6  - https://doi.org/10.1063/1.3675874
SN  - 1089-7674 (E-Journal); 1070-664X (Print)
VL  - 19
IS  - 1
SP  - 1
EP  - 7
PB  - AIP Publishing
CY  - Melville, NY
ER  - 
TY  - JOUR
A1  - Reisgen, Uwe
A1  - Schleser, Markus
A1  - Abdurakhmanov, Aydemir
A1  - Turichin, Gleb
A1  - Valdaitseva, Elena
A1  - Bach, Friedrich-Wilhelm
A1  - Hassel, Thomas
A1  - Beniyashi, Alexander
T1  - Investigation of factors influencing the formation of weld defects in non-vacuum electron beam welding
JF  - The Paton welding journal
N2  - The influence of welding condition parameters and properties of material on formation of defects, such as humping and undercuts, in non-vacuum electron beam welding was investigated. The influence of separate welding parameters on the quality of welds was determined.
Y1  - 2012
SN  - 0957-798X
VL  - 2012
IS  - 2
SP  - 11
EP  - 18
PB  - Paton Publishing House
CY  - Kiev
ER  - 
TY  - JOUR
A1  - Reisgen, Uwe
A1  - Schleser, Markus
A1  - Mokrov, Oleg
A1  - Ahmed, Essam
T1  - Statistical modeling of laser welding of DP/TRIP steel sheets
JF  - Optics and laser technology
N2  - In this research work, a statistical analysis of the CO2 laser beam welding of dual phase (DP600)/transformation induced plasticity (TRIP700) steel sheets was done using response surface methodology. The analysis considered the effect of laser power (2–2.2 kW), welding speed (40–50 mm/s) and focus position (−1 to 0 mm) on the heat input, the weld bead geometry, uniaxial tensile strength, formability limited dome height and welding operation cost. The experimental design was based on Box–Behnken design using linear and quadratic polynomial equations for predicting the mathematical models. The results indicate that the proposed models predict the responses adequately within the limits of welding parameters being used and the welding speed is the most significant parameter during the welding process.
Y1  - 2012
U6  - https://doi.org/10.1016/j.optlastec.2011.05.025
SN  - 1879-2545 (E-Journal); 0030-3992 (Print); 0308-4280 (Print)
VL  - 44
IS  - 1
SP  - 92
EP  - 101
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Reisgen, Uwe
A1  - Schleser, Markus
A1  - Mokrov, Oleg
A1  - Ahmed, Essam
T1  - Optimization of laser welding of DP/TRIP steel sheets using statistical approach
JF  - Optics and laser technology
N2  - Generally, the quality of a weld joint is directly influenced by the welding input parameter settings. Selection of proper process parameters is important to obtain the desired weld bead profile and quality. In this research work, numerical and graphical optimization techniques of the CO2 laser beam welding of dual phase (DP600)/transformation induced plasticity (TRIP700) steel sheets were carried out using response surface methodology (RSM) based on Box–Behnken design. The procedure was established to improve the weld quality, increase the productivity and minimize the total operation cost by considering the welding parameters range of laser power (2–2.2 kW), welding speed (40–50 mm/s) and focus position (−1 to 0 mm). It was found that, RSM can be considered as a powerful tool in experimental welding optimization, even when the experimenter does not have a model for the process. Strong, efficient and low cost weld joints could be achieved using the optimum welding conditions.
Y1  - 2012
U6  - https://doi.org/10.1016/j.optlastec.2011.06.028
SN  - 1879-2545 (E-Journal); 0030-3992 (Print); 0308-4280 (Print)
VL  - 44
IS  - 1
SP  - 255
EP  - 262
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Reisgen, Uwe
A1  - Schleser, Markus
A1  - Mokrov, Oleg
A1  - Zabirov, Alexander
A1  - Füssel, Uwe
A1  - Schnick, Michael
A1  - Hertel, Martin
A1  - Jaeckel, Sebastian
T1  - Modellierung und Visualisierung der MSG-Lichtbogenprozesse
JF  - Schweissen und Schneiden : Fachzeitschrift für Schweißen und verwandte Verfahren
Y1  - 2012
SN  - 0036-7184
VL  - 64
IS  - 4
SP  - 166
EP  - 174
PB  - DVS Verlag
CY  - Düsseldorf
ER  - 
TY  - JOUR
A1  - Reisgen, Uwe
A1  - Schleser, Markus
A1  - Mokrov, Oleg
A1  - Zabirov, Alexander
A1  - Füssel, Uwe
A1  - Schnick, Michael
A1  - Hertel, Martin
A1  - Jaeckel, Sebastian
T1  - Modelling and visualisation of the GMA process
JF  - Welding and Cutting
Y1  - 2012
SN  - 1612-3433
VL  - 11
IS  - 4
SP  - 242
EP  - 249
PB  - DVS Verlag
CY  - Düsseldorf
ER  - 
TY  - JOUR
A1  - Ribitsch, D.
A1  - Heumann, S.
A1  - Karl, W.
A1  - Gerlach, J.
A1  - Leber, R.
A1  - Birner-Gruenberger, R.
A1  - Gruber, K.
A1  - Eiteljoerg, I.
A1  - Remler, P.
A1  - Siegert, Petra
A1  - Lange, J.
A1  - Maurer, Karl-Heinz
A1  - Berg, G.
A1  - Guebitz, G. M.
A1  - Schwab, H.
T1  - Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli
JF  - Journal of biotechnology
N2  - A large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. Several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. Encapsulation of cells in alginate beads induced protease production. Stenotrophomonas maltophilia emerged as best performer under washing conditions. For identification of wash-active proteases, four extracellular serine proteases called StmPr1, StmPr2, StmPr3 and StmPr4 were cloned. StmPr2 and StmPr4 were sufficiently overexpressed in E. coli. Expression of StmPr1 and StmPr3 resulted in unprocessed, insoluble protein. Truncation of most of the C-terminal domain which has been identified by enzyme modeling succeeded in expression of soluble, active StmPr1 but failed in case of StmPr3.

From laundry application tests StmPr2 turned out to be a highly wash-active protease at 45 °C. Specific activity of StmPr2 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 17 ± 2 U/mg. In addition we determined the kinetic parameters and cleavage preferences of protease StmPr2.
KW  - Alginate beads
KW  - Stenotrophomonas maltophilia
KW  - Detergent protease
Y1  - 2012
U6  - https://doi.org/10.1016/j.jbiotec.2011.09.025
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - 157
IS  - 1
SP  - 140
EP  - 147
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Rigling, Andreas
A1  - Bigler, Christof
A1  - Eilmann, Britta
A1  - Feldmeyer-Christe, Elisabeth
A1  - Gimmi, Urs
A1  - Ginzler, Christian
A1  - Graf, Ulrich
A1  - Mayer, Phillip
A1  - Vacchiano, Giorgio
A1  - Weber, Pascal
A1  - Wohlgemuth, Thomas
A1  - zweifel, Roman
A1  - Dobbertin, Matthias
T1  - Driving factors of a vegetation shift from Scots pine to pubescent oak in dry Alpine forests
JF  - Global Change Biology
N2  - An increasing number of studies have reported on forest declines and vegetation shifts triggered by drought. In the Swiss Rhone valley (Valais), one of the driest inner-Alpine regions, the species composition in low elevation forests is changing: The sub-boreal Scots pine (Pinus sylvestris L.) dominating the dry forests is showing high mortality rates. Concurrently the sub-Mediterranean pubescent oak (Quercus pubescens Willd.) has locally increased in abundance. However, it remains unclear whether this local change in species composition is part of a larger-scale vegetation shift. To study variability in mortality and regeneration in these dry forests we analysed data from the Swiss national forest inventory (NFI) on a regular grid between 1983 and 2003, and combined it with annual mortality data from a monitoring site. Pine mortality was found to be highest at low elevation (below 1000 m a.s.l.). Annual variation in pine mortality was correlated with a drought index computed for the summer months prior to observed tree death. A generalized linear mixed-effects model indicated for the NFI data increased pine mortality on dryer sites with high stand competition, particularly for small-diameter trees. Pine regeneration was low in comparison to its occurrence in the overstorey, whereas oak regeneration was comparably abundant. Although both species regenerated well at dry sites, pine regeneration was favoured at cooler sites at higher altitude and oak regeneration was more frequent at warmer sites, indicating a higher adaptation potential of oaks under future warming. Our results thus suggest that an extended shift in species composition is actually occurring in the pine forests in the Valais. The main driving factors are found to be climatic variability, particularly drought, and variability in stand structure and topography. Thus, pine forests at low elevations are developing into oak forests with unknown consequences for these ecosystems and their goods and services.
Y1  - 2012
U6  - https://doi.org/10.1111/gcb.12038
SN  - 1354-1013 (Print)
SN  - 1365-2486 (Online)
VL  - 19
IS  - 1
SP  - 229
EP  - 240
PB  - Wiley-Blackwell
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Rigling, Andreas
A1  - Elkin, Ché
A1  - Dobbertin, Matthias
A1  - Eilmann, Britta
A1  - Giuggiola, Arnaud
A1  - Wohlgemuth, Thomas
A1  - Bugmann, Harald
T1  - Wald und Klimawandel in der inneralpinen Trockenregion Visp
JF  - Schweizerische Zeitschrift für Forstwesen
N2  - Der Temperaturanstieg der vergangenen Jahrzehnte war in Gebirgsregionen besonders ausgeprägt. Sollte sich dieser Temperaturtrend im 21. Jahrhundert fortsetzen, dann dürf-ten sich auch die Häufigkeit und Intensität von Niederschlä-gen und Trockenperioden verändern, was die Waldwirtschaft vor grosse Herausforderungen stellen wird. Trockenheit ist ein entscheidender Faktor für die Waldökosysteme der inner-alpinen Täler, wo seit Jahren trockenheitsbedingtes Baum-sterben festgestellt wird. Der vorliegende Beitrag hat zum Ziel, die Sensitivität der verschiedenen Waldökosysteme ge-genüber Klimawandel und alternative, teilweise extreme Bewirtschaftungsvarianten in der Region Visp zu testen. Me-thodische Ansätze wie Monitoring, Feldexperimente und dy-namische Modellierung wurden kombiniert, und es wurden verschiedene Ökosystemleistungen berücksichtigt, so Holz-produktion, Schutz vor Naturgefahren, Kohlenstoffspeiche-rung und Aspekte der Biodiversität. Die Resultate deuten da-rauf hin, dass auf den Trockenstandorten der Tieflagen die einheimischen Baumarten an ihre physiologischen Grenzen stossen und längerfristig über alternative Baumarten nach-gedacht werden sollte. In den mittleren Lagen dürften Tro-ckenheit und Schadinsekten wie die Borkenkäfer die zukünf-tige Waldentwicklung steuern. In den Hochlagen hingegen werden sich die Wälder ausdehnen und besser wachsen als heute. Alle untersuchten Ökosystemleistungen werden ge-bietsweise starken Veränderungen unterworfen sein. Die dis-kutierten Bewirtschaftungsvarianten scheinen dazu geeignet, die Wasserverfügbarkeit der Bestände, hauptsächlich in tief-eren Lagen, zu erhöhen und dadurch die Widerstandskraft der Bäume gegenüber Trockenperioden vorübergehend zu stärken. Es ist aber davon ausgehen, dass nur verhältnismäs-sig starke Eingriffe wesentliche Effekte erzielen können. Die Verknüpfung von Umweltmonitoring, Feldexperimenten und Modellierung unter Miteinbezug von Ökosystemleistungen ist vielversprechend, da sie eine differenzierte Abschätzung des zukünftigen Landschaftswandels und seiner Effekte auf die Waldleistungen ermöglicht.
Y1  - 2012
U6  - https://doi.org/10.3188/szf.2012.0481
SN  - 2235-1469 (Online)
SN  - 0036-7818 (Print)
VL  - 163
IS  - 12
SP  - 481
EP  - 492
PB  - Schweizerischer Forstverein
CY  - Zürich
ER  - 
TY  - JOUR
A1  - Rost, Kathrin Dorothea
A1  - Lind, Thorsten Patric
T1  - Die Aufrechnung des Insolvenzverwalters gegen eine Insolvenzforderung nach ihrer Feststellung
JF  - Zeitschrift für das gesamte Insolvenzrecht : ZInsO
Y1  - 2012
SN  - 1615-8032
SP  - 2179
EP  - 2187
PB  - Heymanns
CY  - Köln
ER  - 
TY  - JOUR
A1  - Scheer, Nico
A1  - Balimane, Praveen
A1  - Hayward, Michael D.
A1  - Buechel, Sandra
A1  - Kauselmann, Gunther
A1  - Wolf, C. Roland
T1  - Generation and Characterization of a Novel Multidrug Resistance Protein 2 Humanized Mouse Line
JF  - Drug Metabolism and Disposition
N2  - The multidrug resistance protein (MRP) 2 is predominantly expressed in liver, intestine, and kidney, where it plays an important role in the excretion of a range of drugs and their metabolites or endogenous compounds into bile, feces, and urine. Mrp knockout [Mrp2(−/−)] mice have been used recently to study the role of MRP2 in drug disposition. Here, we describe the first generation and initial characterization of a mouse line humanized for MRP2 (huMRP2), which is nulled for the mouse Mrp2 gene and expresses the human transporter in the organs and cell types where MRP2 is normally expressed. Analysis of the mRNA expression for selected cytochrome P450 and transporter genes revealed no major changes in huMRP2 mice compared with wild-type controls. We show that human MRP2 is able to compensate functionally for the loss of the mouse transporter as demonstrated by comparable bilirubin levels in the humanized mice and wild-type controls, in contrast to the hyperbilirubinemia phenotype that is observed in MRP2(−/−) mice. The huMRP2 mouse provides a model to study the role of the human transporter in drug disposition and in assessing the in vivo consequences of inhibiting this transporter by compounds interacting with human MRP2.
Y1  - 2012
U6  - https://doi.org/10.1124/dmd.112.047605
SN  - 1521-0111
VL  - 40
IS  - 11
SP  - 2212
EP  - 2218
PB  - ASPET
CY  - Bethesda, Md.
ER  - 
TY  - JOUR
A1  - Scheer, Nico
A1  - Kapelyukh, Yury
A1  - McEwan, Jillian
A1  - Beuger, Vincent
A1  - Stanley, Lesley A.
A1  - Rode, Anja
A1  - Wolf, C. Roland
T1  - Modeling Human Cytochrome P450 2D6 Metabolism and Drug-drug Interaction by a Novel Panel of Knockout and Humanized Mouse Lines
JF  - Molecular Pharmacology
N2  - The highly polymorphic human cytochrome P450 2D6 enzyme is involved in the metabolism of up to 25% of all marketed drugs and accounts for significant individual differences in response to CYP2D6 substrates. Because of the differences in the multiplicity and substrate specificity of CYP2D family members among species, it is difficult to predict pathways of human CYP2D6-dependent drug metabolism on the basis of animal studies. To create animal models that reflect the human situation more closely and that allow an in vivo assessment of the consequences of differential CYP2D6 drug metabolism, we have developed a novel straightforward approach to delete the entire murine Cyp2d gene cluster and replace it with allelic variants of human CYP2D6. By using this approach, we have generated mouse lines expressing the two frequent human protein isoforms CYP2D6.1 and CYP2D6.2 and an as yet undescribed variant of this enzyme, as well as a Cyp2d cluster knockout mouse. We demonstrate that the various transgenic mouse lines cover a wide spectrum of different human CYP2D6 metabolizer phenotypes. The novel humanization strategy described here provides a robust approach for the expression of different CYP2D6 allelic variants in transgenic mice and thus can help to evaluate potential CYP2D6-dependent interindividual differences in drug response in the context of personalized medicine.
Y1  - 2012
U6  - https://doi.org/10.1124/mol.111.075192
SN  - 1521-0111
VL  - 81
IS  - 1
SP  - 63
EP  - 72
PB  - ASPET
CY  - Bethesda, Md.
ER  - 
TY  - JOUR
A1  - Scheer, Nico
A1  - Kapelyukh, Yury
A1  - Rode, Anja
A1  - Buechel, Sandra
A1  - Wolf, C. Roland
T1  - Generation and characterization of novel cytochrome P450 Cyp2c gene cluster knockout and CYP2C9 humanized mouse lines
JF  - Molecular Pharmacology
N2  - Compared with rodents and many other animal species, the human cytochrome P450 (P450) Cyp2c gene cluster varies significantly in the multiplicity of functional genes and in the substrate specificity of its enzymes. As a consequence, the use of wild-type animal models to predict the role of human CYP2C enzymes in drug metabolism and drug-drug interactions is limited. Within the human CYP2C cluster CYP2C9 is of particular importance, because it is one of the most abundant P450 enzymes in human liver, and it is involved in the metabolism of a wide variety of important drugs and environmental chemicals. To investigate the in vivo functions of cytochrome P450 Cyp2c genes and to establish a model for studying the functions of CYP2C9 in vivo, we have generated a mouse model with a deletion of the murine Cyp2c gene cluster and a corresponding humanized model expressing CYP2C9 specifically in the liver. Despite the high number of functional genes in the mouse Cyp2c cluster and the reported roles of some of these proteins in different biological processes, mice deleted for Cyp2c genes were viable and fertile but showed certain phenotypic alterations in the liver. The expression of CYP2C9 in the liver also resulted in viable animals active in the metabolism and disposition of a number of CYP2C9 substrates. These mouse lines provide a powerful tool for studying the role of Cyp2c genes and of CYP2C9 in particular in drug disposition and as a factor in drug-drug interaction.
Y1  - 2012
U6  - https://doi.org/10.1124/mol.112.080036
SN  - 1521-0111
VL  - 82
IS  - 6
SP  - 1022
EP  - 1029
PB  - ASPET
CY  - Bethesda, Md.
ER  - 
TY  - JOUR
A1  - Scheik, Sven
A1  - Schleser, Markus
A1  - Reisgen, Uwe
T1  - Thermisches Direktfügen von Metall und Kusntstoff: eine Alternative zur Klebtechnik
JF  - Adhäsion : Kleben & Dichten
N2  - Im Rahmen des Exzellenzclusters „Integrative Produktionstechnik für Hochlohnländer“ der RWTHAachen University werden derzeit alternative Verfahren zur Herstellung von Metall/Kunststoff- Verbindungen untersucht. Eines davon ist das thermische Direktfügen, das eine stoffschlüssige Verbindung zwischen Kunststoff und Metall ermöglicht und ohne die Verwendung von Klebstoffen, Haftvermittlern oder mechanischen Verbindungshilfen auskommt.
Y1  - 2012
U6  - https://doi.org/10.1007/s35145-012-0005-x
SN  - 0001-8198 (E-Journal); 0001-8198 (Print)
N1  - Printausg. in der Bibliothek der FH Aachen vorhanden: 63 Z 807-2012
VL  - 56
IS  - 11
SP  - 36
EP  - 40
PB  - Springer Vieweg
CY  - Wiesbaden
ER  -