TY  - JOUR
A1  - Halbach, Thorsten
A1  - Scheer, Nico
T1  - Transcriptional activation by the PHD finger is inhibited through an adjacent leucine zipper that binds 14-3-3 proteins
JF  - Nucleic Acids Research
Y1  - 2000
U6  - http://dx.doi.org/10.1093/nar/28.18.3542
SN  - 1362-4962
VL  - 28
IS  - 18
SP  - 3542
EP  - 3550
ER  - 
TY  - CHAP
A1  - Hahn, Thomas
A1  - Kelly, Svenja
A1  - Muffler, Kai
A1  - Tippkötter, Nils
A1  - Ulber, Roland
ED  - Hans-Jörg, Bart
ED  - Pilz, Stephan
T1  - Extraction of lignocellulose and algae for the production of bulk and fine chemicals
T2  - Industrial scale natural products extraction
Y1  - 2011
SN  - 978-3-527-32504-7 (Print)
SN  - 978-3-527-63512-2 (Online)
U6  - http://dx.doi.org/10.1002/9783527635122
SP  - 221
EP  - 245
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Hager, Jonathan
A1  - Hentschke, Reinhard
A1  - Hojdis, Nils
A1  - Karimi-Varzaneh, Hossein Ali
T1  - Computer Simulation of Particle–Particle Interaction in a Model Polymer Nanocomposite
JF  - Macromolecules
Y1  - 2015
U6  - http://dx.doi.org/10.1021/acs.macromol.5b01864
SN  - 1520-5835
VL  - 48
IS  - 24
SP  - 9039
EP  - 9049
ER  - 
TY  - JOUR
A1  - Hafidi, Youssef
A1  - El Hatka, Hicham
A1  - Schmitz, Dominik
A1  - Krauss, Manuel
A1  - Pettrak, Jürgen
A1  - Biel, Markus
A1  - Ittobane, Najim
T1  - Sustainable soil additives for water and micronutrient supply: swelling and chelating properties of polyaspartic acid hydrogels utilizing newly developed crosslinkers
JF  - Gels
N2  - Drought and water shortage are serious problems in many arid and semi-arid regions. This problem is getting worse and even continues in temperate climatic regions due to climate change. To address this problem, the use of biodegradable hydrogels is increasingly important for the application as water-retaining additives in soil. Furthermore, efficient (micro-)nutrient supply can be provided by the use of tailored hydrogels. Biodegradable polyaspartic acid (PASP) hydrogels with different available (1,6-hexamethylene diamine (HMD) and L-lysine (LYS)) and newly developed crosslinkers based on diesters of glycine (GLY) and (di-)ethylene glycol (DEG and EG, respectively) were synthesized and characterized using Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) and regarding their swelling properties (kinetic, absorbency under load (AUL)) as well as biodegradability of PASP hydrogel. Copper (II) and zinc (II), respectively, were loaded as micronutrients in two different approaches: in situ with crosslinking and subsequent loading of prepared hydrogels. The results showed successful syntheses of di-glycine-ester-based crosslinkers. Hydrogels with good water-absorbing properties were formed. Moreover, the developed crosslinking agents in combination with the specific reaction conditions resulted in higher water absorbency with increased crosslinker content used in synthesis (10% vs. 20%). The prepared hydrogels are candidates for water-storing soil additives due to the biodegradability of PASP, which is shown in an exemple. The incorporation of Cu(II) and Zn(II) ions can provide these micronutrients for plant growth.
KW  - micronutrients
KW  - swelling properties
KW  - biodegradable polymers
KW  - hydrogels
KW  - superabsorbent polymers
KW  - glycine
KW  - polyaspartic acid
Y1  - 2024
U6  - http://dx.doi.org/10.3390/gels10030170
SN  - 2310-2861
VL  - 10
IS  - 3
SP  - Artikel 170
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Haeger, Gerrit
A1  - Grankin, Alina
A1  - Wagner, Michaela
T1  - Construction of an Aspergillus oryzae triple amylase deletion mutant as a chassis to evaluate industrially relevant amylases using multiplex CRISPR/Cas9 editing technology
JF  - Applied Research
N2  - Aspergillus oryzae is an industrially relevant organism for the secretory production of heterologous enzymes, especially amylases. The activities of potential heterologous amylases, however, cannot be quantified directly from the supernatant due to the high background activity of native α-amylase. This activity is caused by the gene products of amyA, amyB, and amyC. In this study, an in vitro CRISPR/Cas9 system was established in A. oryzae to delete these genes simultaneously. First, pyrG of A. oryzae NSAR1 was mutated by exploiting NHEJ to generate a counter-selection marker. Next, all amylase genes were deleted simultaneously by co-transforming a repair template carrying pyrG of Aspergillus nidulans and flanking sequences of amylase gene loci. The rate of obtained triple knock-outs was 47%. We showed that triple knockouts do not retain any amylase activity in the supernatant. The established in vitro CRISPR/Cas9 system was used to achieve sequence-specific knock-in of target genes. The system was intended to incorporate a single copy of the gene of interest into the desired host for the development of screening methods. Therefore, an integration cassette for the heterologous Fpi amylase was designed to specifically target the amyB locus. The site-specific integration rate of the plasmid was 78%, with exceptional additional integrations. Integration frequency was assessed via qPCR and directly correlated with heterologous amylase activity. Hence, we could compare the efficiency between two different signal peptides. In summary, we present a strategy to exploit CRISPR/Cas9 for gene mutation, multiplex knock-out, and the targeted knock-in of an expression cassette in A. oryzae. Our system provides straightforward strain engineering and paves the way for development of fungal screening systems.
KW  - aspergillus
KW  - CRISPR/Cas9
KW  - filamentous fungi
KW  - genome engineering
Y1  - 2023
U6  - http://dx.doi.org/10.1002/appl.202200106
SN  - 2702-4288
IS  - Early View
SP  - 1
EP  - 15
PB  - Wiley-VCH
ER  - 
TY  - JOUR
A1  - Haeger, Gerrit
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
T1  - A convenient ninhydrin assay in 96-well format for amino acid-releasing enzymes using an air-stable reagent
JF  - Analytical Biochemistry
N2  - An improved and convenient ninhydrin assay for aminoacylase activity measurements was developed using the commercial EZ Nin™ reagent. Alternative reagents from literature were also evaluated and compared. The addition of DMSO to the reagent enhanced the solubility of Ruhemann's purple (RP). Furthermore, we found that the use of a basic, aqueous buffer enhances stability of RP. An acidic protocol for the quantification of lysine was developed by addition of glacial acetic acid. The assay allows for parallel processing in a 96-well format with measurements microtiter plates.
Y1  - 2022
U6  - http://dx.doi.org/10.1016/j.ab.2022.114819
SN  - 1096-0309
IS  - 624
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Guo, Yuanyuan
A1  - Miyamoto, Ko-ichiro
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
A1  - Yoshinobu, Tatsuo
T1  - Theoretical study and simulation of light-addressable potentiometric sensors
JF  - Physica status solidi (A) : applications and materials
N2  - The light-addressable potentiometric sensor (LAPS) is a semiconductor-based potentiometric sensor using a light probe with an ability of detecting the concentration of biochemical species in a spatially resolved manner. As an important biomedical sensor, research has been conducted to improve its performance, for instance, to realize high-speed measurement. In this work, the idea of facilitating the device-level simulation, instead of using an equivalent-circuit model, is presented for detailed analysis and optimization of the performance of the LAPS. Both carrier distribution and photocurrent response have been simulated to provide new insight into both amplitude-mode and phase-mode operations of the LAPS. Various device parameters can be examined to effectively design and optimize the LAPS structures and setups for enhanced performance.
Y1  - 2014
U6  - http://dx.doi.org/10.1002/pssa.201330354
SN  - 0031-8965
VL  - 211
IS  - 6
SP  - 1467
EP  - 1472
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - INPR
A1  - Greiner, Lasse
A1  - Jeromin, Günter Erich
A1  - Sithole, Patience
A1  - Petersen, Soenke
T1  - Preprint: Studies on the enzymatic reduction of levulinic acid using Chiralidon-R and Chiralidon-S
T2  - ChemRxiv
N2  - The enzymatic reduction of levulinic acid by the chiral catalysts Chiralidon-R and Chiralidon-S which are commercially available superabsorbed alcohol dehydrogenases is described. The Chiralidon®-R/S reduces the levulinic acid to the (R,S)-4-hydroxy valeric acid and the (R)- or (S)- gamma-valerolactone.
KW  - Levulinic acid
KW  - Chiralidon-R
KW  - Chiralidon-S
KW  - 4-hydroxy valeric acid
KW  - (R)- or (S)- gamma-valerolactone
Y1  - 2023
U6  - http://dx.doi.org/10.26434/chemrxiv-2023-jlvcv
ER  - 
TY  - JOUR
A1  - Goetze, Sandra
A1  - Baer, Alexandra
A1  - Winkelmann, Silke
A1  - Nehlsen, Kristina
A1  - Seibler, Jost
A1  - Maass, Karin
A1  - Bode, Jürgen
T1  - Performance of genomic bordering elements at predefined genomic loci
JF  - Molecular and Cellular Biology
Y1  - 2005
U6  - http://dx.doi.org/10.1128/MCB.25.6.2260-2272.2005
SN  - 1098-5549
VL  - 25
IS  - 6
SP  - 2260
EP  - 2272
ER  - 
TY  - JOUR
A1  - Glaser, Stefan
A1  - Lubitz, Sandra
A1  - Loveland, Kate L.
A1  - Ohbo, Kazu
A1  - Robb, Lorraine
A1  - Schwenk, Frieder
A1  - Seibler, Jost
A1  - Roellig, Daniela
A1  - Kranz, Andrea
A1  - Anastassiadis, Konstantinos
A1  - Stewart, A. Francis
T1  - The histone 3 lysine 4 methyltransferase, Mll2, is only required briefly in development and spermatogenesis
JF  - Epigenetics & Chromatin
Y1  - 2009
U6  - http://dx.doi.org/10.1186/1756-8935-2-5
SN  - 1756-8935
VL  - 2
IS  - 5
SP  - 1
EP  - 16
ER  - 
TY  - BOOK
A1  - Gilch, Heinz Gerhard
A1  - Rath, Walter
T1  - Moisture-curing, polyurethane hot-melt compositions = Feuchtigkeitshärtende Polyurethan-Schmelzklebstoff-Zusammensetzungen
Y1  - 1995
N1  - EP 0369607 30.03.1995 Veröffentlichungstag im Patentblatt ; Volltext recherchierbar über:
PB  - Europäisches Patentamt
CY  - München
ER  - 
TY  - JOUR
A1  - Ghosch, S.
A1  - Baier, M.
A1  - Schütz, J.
A1  - Schneider, Felix
A1  - Scherer, Ulrich W.
T1  - Analysis of electronic autoradiographs by mathematical post-processing
JF  - Radiation Effects and Defects in Solids: Incorporating plasma science and plasma technology
N2  - Autoradiography is a well-established method of nuclear imaging. When different radionuclides are present simultaneously, additional processing is needed to distinguish distributions of radionuclides. In this work, a method is presented where aluminium absorbers of different thickness are used to produce images with different cut-off energies. By subtracting images pixel-by-pixel one can generate images representing certain ranges of β-particle energies. The method is applied to the measurement of irradiated reactor graphite samples containing several radionuclides to determine the spatial distribution of these radionuclides within pre-defined energy windows. The process was repeated under fixed parameters after thermal treatment of the samples. The greyscale images of the distribution after treatment were subtracted from the corresponding pre-treatment images. Significant changes in the intensity and distribution of radionuclides could be observed in some samples. Due to the thermal treatment parameters the most significant differences were observed in the ³H and ¹⁴C inventory and distribution.
Y1  - 2016
U6  - http://dx.doi.org/10.1080/10420150.2016.1155587
SN  - 1029-4953
VL  - 171
IS  - 1-2
SP  - 161
EP  - 172
PB  - Taylor & Francis
CY  - London
ER  - 
TY  - JOUR
A1  - Gerigk, M.
A1  - Maaß, D.
A1  - Kreutzer, A.
A1  - Sprenger, G.
A1  - Bongaerts, Johannes
A1  - Wubbolts, Marcel
A1  - Takors, Ralf
T1  - Enhanced pilot-scale fed-batch L-phenylalanine production with recombinant Escherichia coli by fully integrated reactive extraction
JF  - Bioprocess and biosystems engineering
Y1  - 2002
SN  - 1432-0797 (E-Journal); 1615-7605 (E-Journal); 0178-515X (Print); 1615-7591 (Print)
VL  - Vol. 25
IS  - Iss. 1
SP  - 43
EP  - 52
ER  - 
TY  - JOUR
A1  - Gerigk, M.
A1  - Bujnicki, R.
A1  - Ganpo-Nkwenkwa, E.
A1  - Bongaerts, Johannes
A1  - Sprenger, G.
A1  - Takors, Ralf
T1  - Process control for enhanced L-phenylalanine production using different recombinant Escherichia coli strains
JF  - Biotechnology and bioengineering
Y1  - 2002
SN  - 1097-0290 (E-Journal); 0006-3592 (Print)
VL  - Vol. 80
IS  - Iss. 7
SP  - 746
EP  - 754
ER  - 
TY  - JOUR
A1  - Gebeshuber, Christoph A.
A1  - Kornauth, Christoph
A1  - Dong, Lihua
A1  - Sierig, Ralph
A1  - Seibler, Jost
A1  - Reiss, Martina
A1  - Tauber, Stefanie
A1  - Bilban, Martin
A1  - Wang, Shijun
A1  - Kain, Renate
A1  - Böhmig, Georg A.
A1  - Moeller, Marcus J.
A1  - Gröne, Hermann-Josef
A1  - Englert, Christoph
A1  - Martinez, Javier
A1  - Kerjaschki, Dontscho
T1  - Focal segmental glomerulosclerosis is induced by microRNA-193a and its downregulation of WT1
JF  - Nature Medicine
Y1  - 2013
U6  - http://dx.doi.org/10.1038/nm.3142
SN  - 1078-8956
VL  - 19
IS  - 4
SP  - 481
EP  - 487
ER  - 
TY  - CHAP
A1  - Frotscher, Ralf
A1  - Goßmann, Matthias
A1  - Raatschen, Hans-Jürgen
A1  - Temiz Artmann, Aysegül
A1  - Staat, Manfred
T1  - Simulation of cardiac cell-seeded membranes using the edge-based smoothed FEM
T2  - Shell and membrane theories in mechanics and biology. (Advanced structured materials ; 45)
N2  - We present an electromechanically coupled Finite Element model for cardiac tissue. It bases on the mechanical model for cardiac tissue of Hunter et al. that we couple to the McAllister-Noble-Tsien electrophysiological model of purkinje fibre cells. The corresponding system of ordinary differential equations is implemented on the level of the constitutive equations in a geometrically and physically nonlinear version of the so-called edge-based smoothed FEM for plates. Mechanical material parameters are determined from our own pressure-deflection experimental setup. The main purpose of the model is to further examine the experimental results not only on mechanical but also on electrophysiological level down to ion channel gates. Moreover, we present first drug treatment simulations and validate the model with respect to the experiments.
Y1  - 2015
SN  - 978-3-319-02534-6 ; 978-3-319-02535-3
SP  - 187
EP  - 212
PB  - Springer
CY  - Heidelberg
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Stahlberg, R.
T1  - Thermoselect – Energie- und Rohstoffgewinnung: Teil 1. Verfahrensgrundlagen zur unterbrechungslosen Verwertung von Restabfällen. Stahlberg, R. ; Feuerriegel, U.
JF  - Chemische Technik <Leipzig>. 46 (1994), H. 5
Y1  - 1994
SN  - 0045-6519
SP  - 257
EP  - 266
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Stahlberg, R.
T1  - Performance of the Thermoselect Demonstration Plant at Fondotoce, Italy: Product Quality and Distribution of Chemical Elements in the Material Flow. Stahlberg, R. ; Feuerriegel, U.
JF  - Solid waste management: thermal treatment & waste-to-energy technologies : proceedings of an international specialty conference sponsored by the Air & Waste Management Association, [Washington, DC, April 18 - 21, 1995] / comp. by James D. Kilgroe
Y1  - 1996
N1  - International Conference on Solid Waste Management, Thermal Treatment and Waste to Energy ; <1995, Washington, DC>
PB  - Air & Waste Management Assoc.
CY  - Pittsburgh, Pa.
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Klose, W.
A1  - Sloboshanin, S.
A1  - Goebel, H. [u.a.]
T1  - Deactivation of a palladium-supported alumina catalyst by hydrogen sulfide during the oxidation of methane
JF  - Langmuir: the ACS journal of surfaces and colloids. 10 (1994), H. 10
Y1  - 1994
SN  - 0743-74363
SP  - 3567
EP  - 3570
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Klose, W.
T1  - Investigations into the Mechanism of the Deactivation of a Palladium-Supported Catalyst by Hydrogen Sulfide Poisoning. Klose, W. ; Feuerriegel, U.
JF  - Chemical engineering & technology. 21 (1998), H. 7
Y1  - 1998
SN  - 0930-7516
N1  - gleicher Artikel in Deutsch In: Chem.Ing.Tech 70(1998) No  S. 449-452 u.d.T.: Untersuchungen zum Mechanismus der Desaktivierung eines Palladium-Trägerkatalysators durch Vergiftung mit Schwefelwasserstoff
SP  - 562
EP  - 564
ER  -