TY - PAT A1 - Berndt, Heinz A1 - Höcker, Hartwig A1 - Kuropka, Rolf A1 - Kinkel, Joachim T1 - Silane coated inorganic materials for chromatography : United States Patent Y1 - 1991 PB - [The United States Patent and Trademark Office] CY - [Alexandria, VA u.a.] ER - TY - JOUR A1 - Wilson, Ian D. A1 - Wilson, Claire E. A1 - Scheer, Nico A1 - Dickie, A.P. A1 - Schreiter, K. A1 - Wilson, E. M. A1 - Riley, R. J. A1 - Wehr, R. A1 - Bial, J. T1 - The Pharmacokinetics and Metabolism of Lumiracoxib in Chimeric Humanized and Murinized FRG Mice JF - Biochemical pharmacology Y1 - 2017 U6 - https://doi.org/10.1016/j.bcp.2017.03.015 SN - 1873-2968 VL - Volume 135 SP - 139 EP - 150 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Hough, Lindsay B. A1 - Nalwalk, Julia W. A1 - Ding, Xinxin A1 - Scheer, Nico T1 - Opioid Analgesia in P450 Gene Cluster Knockout Mice: A Search for Analgesia-Relevant Isoforms JF - Drug Metabolism and Disposition Y1 - 2015 U6 - https://doi.org/10.1124/dmd.115.065490 SN - 1521-009x VL - 43 IS - 9 SP - 1326 EP - 1330 ER - TY - JOUR A1 - Mues genannt Koers, Lucas A1 - McNeil, S. W. A1 - Radchenko, V. A1 - Paulßen, Elisabeth A1 - Hoehr, Cornelia T1 - Production of Co-58m in a siphon-style liquid target on a medical cyclotron N2 - We present the production of 58mCo on a small, 13 MeV medical cyclotron utilizing a siphon style liquid target system. Different concentrated iron(III)-nitrate solutions of natural isotopic distribution were irradiated at varying initial pressures and subsequently separated by solid phase extraction chromatography. The radio cobalt (58m/gCo and 56Co) was successfully produced with saturation activities of (0.35 ± 0.03) MBq μA−1 for 58mCo with a separation recovery of (75 ± 2) % of cobalt after one separation step utilizing LN-resin. Y1 - 2023 U6 - https://doi.org/10.1016/j.apradiso.2023.110734 SN - 0969-8043 VL - 195 IS - Art. 110734 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Hafidi, Youssef A1 - El Hatka, Hicham A1 - Schmitz, Dominik A1 - Krauss, Manuel A1 - Pettrak, Jürgen A1 - Biel, Markus A1 - Ittobane, Najim T1 - Sustainable soil additives for water and micronutrient supply: swelling and chelating properties of polyaspartic acid hydrogels utilizing newly developed crosslinkers JF - Gels N2 - Drought and water shortage are serious problems in many arid and semi-arid regions. This problem is getting worse and even continues in temperate climatic regions due to climate change. To address this problem, the use of biodegradable hydrogels is increasingly important for the application as water-retaining additives in soil. Furthermore, efficient (micro-)nutrient supply can be provided by the use of tailored hydrogels. Biodegradable polyaspartic acid (PASP) hydrogels with different available (1,6-hexamethylene diamine (HMD) and L-lysine (LYS)) and newly developed crosslinkers based on diesters of glycine (GLY) and (di-)ethylene glycol (DEG and EG, respectively) were synthesized and characterized using Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) and regarding their swelling properties (kinetic, absorbency under load (AUL)) as well as biodegradability of PASP hydrogel. Copper (II) and zinc (II), respectively, were loaded as micronutrients in two different approaches: in situ with crosslinking and subsequent loading of prepared hydrogels. The results showed successful syntheses of di-glycine-ester-based crosslinkers. Hydrogels with good water-absorbing properties were formed. Moreover, the developed crosslinking agents in combination with the specific reaction conditions resulted in higher water absorbency with increased crosslinker content used in synthesis (10% vs. 20%). The prepared hydrogels are candidates for water-storing soil additives due to the biodegradability of PASP, which is shown in an exemple. The incorporation of Cu(II) and Zn(II) ions can provide these micronutrients for plant growth. KW - micronutrients KW - swelling properties KW - biodegradable polymers KW - hydrogels KW - superabsorbent polymers KW - glycine KW - polyaspartic acid Y1 - 2024 U6 - https://doi.org/10.3390/gels10030170 SN - 2310-2861 VL - 10 IS - 3 SP - Artikel 170 PB - MDPI CY - Basel ER - TY - GEN A1 - Roth, J. A1 - Tippkötter, Nils T1 - New Approach for Enzymatic Hydrolysis of Lignocellulose with Selective Diffusion Separation of the Monosaccharide Products T2 - Chemie Ingenieur Technik N2 - Enzymatic hydrolysis of lignocellulosic material plays an important role in the classical biorefinery approach. Apart from the pretreatment of the raw material, hydrolysis is the basis for the conversion of the cellulose and hemicellulose fraction into fermentable sugars. After hydrolysis, usually a solid-liquid separation takes place, in order to separate the residual plant material from the sugar-rich fraction, which can be subsequently used in a fermentation step. In order to factor out the separation step, the usage of in alginate immobilized crude cellulose fiber beads (CFBs) were evaluated. Pretreated cellulose fibers are incorporated in an alginate matrix together with the relevant enzymes. In doing so, sugars diffuse trough the alginate matrix, allowing a simplified delivery into the surrounding fluid. This again reduces product inhibition of the glucose on the enzyme catalysts. By means of standardized bead production the hydrolysis in lab scale was possible. First results show that liberation of glucose and xylose is possible, allowing a maximum total sugar yield of 75 %. Y1 - 2016 U6 - https://doi.org/10.1002/cite.201650301 SN - 0009-286X SN - 1522-2640 (eISSN) N1 - ProcessNet-Jahrestagung 2016 und 32. DECHEMA-Jahrestagung der Biotechnologen 2016, 12. - 15. September 2016, Eurogress Aachen VL - 88 IS - 9 SP - 1237 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Oehlenschläger, Katharina A1 - Volkmar, Marianne A1 - Stiefelmaier, Judith A1 - Langsdorf, Alexander A1 - Holtmann, Dirk A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - New insights into the influence of pre-culture on robust solvent production of C. acetobutylicum JF - Applied Microbiology and Biotechnology N2 - Clostridia are known for their solvent production, especially the production of butanol. Concerning the projected depletion of fossil fuels, this is of great interest. The cultivation of clostridia is known to be challenging, and it is difficult to achieve reproducible results and robust processes. However, existing publications usually concentrate on the cultivation conditions of the main culture. In this paper, the influence of cryo-conservation and pre-culture on growth and solvent production in the resulting main cultivation are examined. A protocol was developed that leads to reproducible cultivations of Clostridium acetobutylicum. Detailed investigation of the cell conservation in cryo-cultures ensured reliable cell growth in the pre-culture. Moreover, a reason for the acid crash in the main culture was found, based on the cultivation conditions of the pre-culture. The critical parameter to avoid the acid crash and accomplish the shift to the solventogenesis of clostridia is the metabolic phase in which the cells of the pre-culture were at the time of inoculation of the main culture; this depends on the cultivation time of the pre-culture. Using cells from the exponential growth phase to inoculate the main culture leads to an acid crash. To achieve the solventogenic phase with butanol production, the inoculum should consist of older cells which are in the stationary growth phase. Considering these parameters, which affect the entire cultivation process, reproducible results and reliable solvent production are ensured. KW - Pre-culture KW - Metabolic shift KW - Acid crash KW - C. acetobutylicum KW - ABE KW - Butanol Y1 - 2024 U6 - https://doi.org/10.1007/s00253-023-12981-8 SN - 1432-0614 VL - 108 PB - Springer CY - Berlin, Heidelberg ER - TY - CHAP A1 - Kalbe, Jochen A1 - Kuropka, Rolf A1 - Meyer-Stork, L. Sebastian A1 - Lauter, S. L. A1 - Höcker, Hartwig A1 - Berndt, Heinz ED - Körner, Andrea T1 - Identification of fine animal hair via DNA analysis T2 - Proceedings of the 1st International Symposium on Specialty Animal Fibers : Aachen, October 26 - 27, 1987 ; [scientific, technological and economical aspects] . - (Schriftenreihe des Deutschen Wollforschungsinstitutes an der Technischen Hochschule Aachen e.V. ; 103) Y1 - 1988 SN - 0930-3723 SP - 221 EP - 227 PB - Dt. Wollforschungsinst. CY - Aachen ER - TY - JOUR A1 - Krüger, Götz A1 - Grötzinger, Joachim A1 - Berndt, Heinz T1 - Enantiomeric resolution of amino acid derivatives on chiral stationary phases by high-performance liquid chromatography JF - Journal of Chromatography A Y1 - 1987 U6 - https://doi.org/10.1016/S0021-9673(01)85005-6 SN - 0021-9673 VL - 1987 IS - 397 SP - 223 EP - 232 ER - TY - JOUR A1 - Nokihara, Kiyoshi A1 - Berndt, Heinz T1 - Synthesis of hapten–polypeptide conjugates as antigen models for the N-terminal region of the α-2-chain of rabbit skin collagen JF - Journal of the Royal Society of Chemistry: Perkin Transactions 1 N2 - Synthesis of derivatives of the peptide sequence L-pyroglutamyl-L-phenylalanyl-L-aspartyl-glycyl-L-lysyl-glycyl-glycyl-glycine as the antigenic determinant representing the N-terminal non-helical region of the α-2-chain of rabbit skin collagen, and conjugation to two different polypeptide carriers, are described. Y1 - 1978 U6 - https://doi.org/10.1039/P19780000260 SN - 1364-5463 SN - 0300-922X SN - 1470-4358 VL - 1978 IS - 3 SP - 260 EP - 263 PB - Royal Society of Chemistry CY - Cambridge ER - TY - CHAP A1 - Kroggel, Matthias A1 - Berndt, Heinz ED - Ragnarsson, Ulf T1 - The 0-hydroxiphenyloxicarbonyl-group a new base labile amine protecting group T2 - Peptides 1984 : Proceedings of the 18th European Peptide Symposium Djurönäset, Sweden, June 10 - 15, 1984 Y1 - 1984 SN - 91-22-00715-6 SP - 81 EP - 83 PB - Almquist & Wiksell CY - Stockholm ER - TY - JOUR A1 - Scheer, Nico A1 - Kapelyukh, Yury A1 - Rode, Anja A1 - Buechel, Sandra A1 - Wolf, C. Roland T1 - Generation and characterization of novel cytochrome P450 Cyp2c gene cluster knockout and CYP2C9 humanized mouse lines JF - Molecular Pharmacology N2 - Compared with rodents and many other animal species, the human cytochrome P450 (P450) Cyp2c gene cluster varies significantly in the multiplicity of functional genes and in the substrate specificity of its enzymes. As a consequence, the use of wild-type animal models to predict the role of human CYP2C enzymes in drug metabolism and drug-drug interactions is limited. Within the human CYP2C cluster CYP2C9 is of particular importance, because it is one of the most abundant P450 enzymes in human liver, and it is involved in the metabolism of a wide variety of important drugs and environmental chemicals. To investigate the in vivo functions of cytochrome P450 Cyp2c genes and to establish a model for studying the functions of CYP2C9 in vivo, we have generated a mouse model with a deletion of the murine Cyp2c gene cluster and a corresponding humanized model expressing CYP2C9 specifically in the liver. Despite the high number of functional genes in the mouse Cyp2c cluster and the reported roles of some of these proteins in different biological processes, mice deleted for Cyp2c genes were viable and fertile but showed certain phenotypic alterations in the liver. The expression of CYP2C9 in the liver also resulted in viable animals active in the metabolism and disposition of a number of CYP2C9 substrates. These mouse lines provide a powerful tool for studying the role of Cyp2c genes and of CYP2C9 in particular in drug disposition and as a factor in drug-drug interaction. Y1 - 2012 U6 - https://doi.org/10.1124/mol.112.080036 SN - 1521-0111 VL - 82 IS - 6 SP - 1022 EP - 1029 PB - ASPET CY - Bethesda, Md. ER - TY - JOUR A1 - Kalbe, Jochen A1 - Kuropka, Rolf A1 - Meyer-Stork, L. Sebastian A1 - Berndt, Heinz A1 - [u.a.], T1 - Isolation and characterization of high-molecular mass DNA from hair shafts JF - Biological chemistry Y1 - 1988 SN - 0177-3593 U6 - https://doi.org/10.1515/bchm3.1988.369.1.413 VL - 369 IS - 1 SP - 413 EP - 416 ER - TY - JOUR A1 - Berndt, Heinz A1 - Krüger, Götz T1 - Resolution of enantiomeric amino acid derivatives by high-performance liquid chromatography on chiral stationary phases JF - Journal of chromatography A Y1 - 1985 U6 - https://doi.org/10.1016/S0021-9673(01)92461-6 SN - 0021-9673 VL - 1985 IS - 348 SP - 275 EP - 279 ER - TY - JOUR A1 - Kuropka, Rolf A1 - Müller, Bettina A1 - Höcker, Hartwig A1 - Berndt, Heinz T1 - Chiral stationary phases via hydrosilylation reaction of N-acryloylamino acids : I. Stationary phase with one chiral centre for high-performance liquid chromatography and development of a new derivatization pattern for amino acid enantiomers JF - Journal of chromatography A Y1 - 1989 SN - 0021-9673 IS - 481 SP - 380 EP - 386 ER - TY - JOUR A1 - Nokihara, Kiyoshi A1 - Berndt, Heinz T1 - Studies on sulfur-containing peptides : tert-butyloxycarbonylsulfenyl and benzyloxycarbonylsulfenyl derivatives as protecting groups for cysteine JF - The journal of organic chemistry Y1 - 1978 U6 - https://doi.org/10.1021/jo00419a046 SN - 0022-3263 VL - 43 IS - 25 SP - 4893 EP - 4895 PB - American Chemical Society CY - Washington ER - TY - JOUR A1 - Kalbe, Jochen A1 - Höcker, Hartwig A1 - Berndt, Heinz T1 - Design of enzyme reactors as chromatographic columns for racemic resolution of amino acid esters JF - Chromatographia Y1 - 1989 SN - 0009-5893 U6 - https://doi.org/10.1007/BF02319646 VL - 28 IS - 3-4 SP - 193 EP - 196 ER -