TY - CHAP A1 - Fricke, Barbara A1 - Hoffschmidt, Bernhard T1 - Ecobalance of a solar thermal tower power plant with volumetric receiver T2 - SolarPACES 2010 : the CSP Conference: electricity, fuels and clean water from concentrated solar energy ; 21 to 24 September 2010, Perpignan, France Y1 - 2010 SP - 87 EP - 88 PB - Soc. OSC CY - Saint Maur ER - TY - CHAP A1 - Baumann, T. A1 - Teixeira Boura, Cristiano José A1 - Göttsche, Joachim A1 - Hoffschmidt, Bernhard A1 - O'Connell, B. A1 - Schmitz, S. A1 - Zunft, S. T1 - Air/Sand heat exchanger design and materials for solar thermal power plant applications T2 - SolarPACES 2010 : the CSP Conference: electricity, fuels and clean water from concentrated solar energy ; 21 to 24 September 2010, Perpignan, France Y1 - 2010 SP - 146 EP - 147 PB - Soc. OSC CY - Saint Maur ER - TY - CHAP A1 - Alexopoulos, Spiros A1 - Hoffschmidt, Bernhard A1 - Rau, Christoph A1 - Schmitz, M. A1 - Schwarzbözl, P. A1 - Pomp, Stefan T1 - Simulation results for a hybridized operation of a gas turbine or a burner for a small solar tower power plant T2 - SolarPACES 2010 : the CSP Conference: electricity, fuels and clean water from concentrated solar energy ; 21 to 24 September 2010, Perpignan, France Y1 - 2010 SP - 82 EP - 83 PB - Soc. OSC CY - Saint Maur ER - TY - CHAP A1 - Buck, R. A1 - Wurmhöringer, K. A1 - Lehle, R. A1 - Pfahl, A. A1 - Göttsche, Joachim A1 - Meyr, T. T1 - Development of a 30m2 heliostat with hydraulic drive T2 - SolarPACES 2010 : the CSP Conference: electricity, fuels and clean water from concentrated solar energy ; 21 to 24 September 2010, Perpignan, France Y1 - 2010 SP - 74 EP - 75 PB - Soc. OSC CY - Saint Maur ER - TY - CHAP A1 - Schwarzbözl, Peter A1 - Pomp, Stefan A1 - Koll, Gerrit A1 - Hennecke, Klaus A1 - Hartz, Thomas A1 - Schmitz, Mark A1 - Hoffschmidt, Bernhard T1 - The solar tower Jülich - first operational experiences and test results T2 - SolarPACES 2010 : the CSP Conference: electricity, fuels and clean water from concentrated solar energy ; 21 to 24 September 2010, Perpignan, France Y1 - 2010 SP - 8 EP - 9 PB - Soc. OSC CY - Saint Maur ER - TY - CHAP A1 - Pomp, Stefan A1 - Schwarzbözl, Peter A1 - Koll, Gerrit A1 - Hennecke, Klaus A1 - Schmitz, Mark A1 - Hoffschmidt, Bernhard T1 - Advanced concept for a solar thermal power plant with open volumetric air receiver T2 - SolarPACES 2010 : the CSP Conference: electricity, fuels and clean water from concentrated solar energy ; 21 to 24 September 2010, Perpignan, France Y1 - 2010 SP - 97 EP - 98 PB - Soc. OSC CY - Saint Maur ER - TY - CHAP A1 - Reisgen, Uwe A1 - Schoene, Jens A1 - Schleser, Markus A1 - Jerabek, Jakub A1 - Keil, Allessandra ED - Brameshuber, Wolfgang T1 - Theoretical and experimental investigations on textile-reinforced concrete spalling behavior T2 - Textile reinforced concrete : 2nd ICTRC : International RILEM Conference on Material Science : Aachen Germany, September 6 - 8, 2010. Vol. 1. (RILEM Proceedings PRO ; 75) Y1 - 2010 SN - 978-2-35158-106-3 SP - 153 EP - 162 PB - RILEM Publ. CY - Bagneux ER - TY - CHAP A1 - Schoene, Jens A1 - Reisgen, Uwe A1 - Schleser, Markus A1 - Jerabek, Jakub A1 - Chudoba, Rostislav ED - Fisher, Gregor T1 - Theoretical and experimental investigations on textile-reinforced concrete spalling behavior T2 - 8th International PhD Symposium in Civil Engineering : Lyngby, June 20-23, 2010 : proceedings Y1 - 2010 SN - 9788778773012 SP - 383 EP - 388 PB - Technical University of Denmark, Dept. of Civil Engineering CY - Lyngby ER - TY - CHAP A1 - Goldbach, Daniel A1 - Ament, Christoph ED - Scharff, Peter T1 - Stabilized reversing with vehicle trailer combinations T2 - Crossing borders within the ABC : automation, biomedical engineering and computer science; proceedings; 55. IWK Internationales Wissenschaftliches Kolloquium; 13 - 17 September 2010, Ilmenau Y1 - 2010 SN - 978-3-938843-53-6 SP - 352 EP - 357 PB - Verl. ISLE CY - Ilmenau ER - TY - CHAP A1 - Bung, Daniel B. A1 - Oertel, M. A1 - Schlenkhoff, A. A1 - Schlurmann, Torsten ED - Obrusnik, Ivan T1 - Flash flood awareness and prevention in Germany T2 - Early warning for flash floods : international workshop, Praha 2011 : workshop proceedings Y1 - 2010 SN - 978-80-86690-91-9 SP - 34 EP - 40 ER - TY - CHAP A1 - Heinz, G. A1 - Oertel, Mario A1 - Bung, Daniel B. ED - Janssen, Robert T1 - Investigations on a cooling water system for a gas tubine facility concerning air vortexes and sediment transport T2 - Hydraulic structures: useful water harvesting systems or relics? : Third International Junior Researcher and Engineer Workshop on Hydraulic Structures (IJREWHS'10) : Edinburgh, Scotland, U.K., 2-3 May 2010 Y1 - 2010 SN - 9781742720159 SP - 109 EP - 116 PB - School of Civil Engineering, The University of Queensland CY - Brisbane ER - TY - CHAP A1 - Kerpen, N. B. A1 - Bung, Daniel B. A1 - Schlurmann, Torsten ED - Janssen, Robert T1 - Physical model investigations of ships passing through a lock T2 - Hydraulic structures: useful water harvesting systems or relics? : Third International Junior Researcher and Engineer Workshop on Hydraulic Structures (IJREWHS'10) : Edinburgh, Scotland, U.K., 2-3 May 2010 Y1 - 2010 SN - 9781742720159 SP - 93 EP - 100 PB - School of Civil Engineeering, The University of Queensland CY - Brisbane ER - TY - CHAP A1 - Bung, Daniel B. A1 - Schlenkhoff, Andreas T1 - Self-aerated skimming flow on embankment stepped spillways : the effect of additional micro-roughness on energy dissipation and oxygen transfer T2 - Proceedings from first IAHR European congress : May 2010, Edinburgh Y1 - 2010 SN - 9780956595102 SP - Artikelkennnummer HSIIId PB - Heriot-Watt University, School of the Built Environment CY - Edinburgh ER - TY - CHAP A1 - Kerpen, N. B. A1 - Bung, Daniel B. A1 - Schlurmann, Torsten T1 - Physical model investigations of pressure distributions next to ships passing through a lock T2 - 5th Chinese-German Joint Symposium on Hydraulic and Ocean Engineering : CG JOINT 2010 Y1 - 2010 SN - 978-7-5618-3671-2 SP - 514 EP - 519 PB - Univ. Press CY - Tianjin ER - TY - CHAP A1 - Bung, Daniel B. T1 - A comparative study of self-aerated stepped spillway and smooth invert chute flow: the effect of step-induced macro roughness T2 - 5th Chinese-German Joint Symposium on Hydraulic and Ocean Engineering : CG JOINT 2010 Y1 - 2010 SN - 978-7-5618-3671-2 SP - 451 EP - 456 PB - Univ. Press CY - Tianjin ER - TY - CHAP A1 - Feldhusen, Jörg A1 - Brezing, Alexander Nikolaus A1 - Pütz, Claus A1 - Wählisch, Georg T1 - Multi-system CAD-teaching in large classes T2 - When design education and design research meet : proceedings of the 12th International Conference on Engineering and Product Design Education, Norwegian University of Science and Technology (NTNU) Trondheim, Norway, 2nd - 3rd September 2010 ; [E&PDE] Y1 - 2010 SN - 978-1-904670-19-3 SP - 204 EP - 209 PB - Design Society CY - Glasgow ER - TY - CHAP A1 - Gligorevic, Snjezana A1 - Pulini, Paola T1 - Simplified airport surface channel model based on the WSSUS assumption T2 - 2010 Integrated Communications, Navigation and Surveillance Conference (ICNS 2010) : Herndon, Virginia, USA, 11 - 13 May 2010 Y1 - 2010 SN - 978-1-4244-7457-8 SN - 2155-4943 SP - F2-1 EP - F2-11 ER - TY - CHAP A1 - Schnell, Michael A1 - Franzen, Nico A1 - Gligorevic, Snjezana T1 - L-DACS1 laboratory demonstrator development and compatibility measurement set-up T2 - IEEE/AIAA 29th Digital Avionics Systems Conference (DASC) : 3 - 7 Oct. 2010, Salt Lake City, Utah Y1 - 2010 SN - 9781424466160 ; 9781424466184 SP - 3E3-1 EP - 3E3-11 ER - TY - CHAP A1 - Walter, Michael A1 - Gligorevic, Snjezana A1 - Detert, Thorben A1 - Schnell, Michael T1 - UHF/VHF air-to-air propagation measurements T2 - Proceedings of the Fourth European Conference on Antennas and Propagation (EuCAP) : 12 - 16 April 2010, Barcelona, Spain Y1 - 2010 SN - 978-1-4244-6431-9 ; 978-84-7653-472-4 SP - 1 EP - 5 ER - TY - JOUR A1 - Ribitsch, D. A1 - Karl, W. A1 - Birner-Gruenberger, R. A1 - Gruber, K. A1 - Eiteljoerg, I. A1 - Remler, P. A1 - Wieland, S. A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schwab, H. T1 - C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli JF - Journal of biotechnology N2 - Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7–11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease. Y1 - 2010 U6 - http://dx.doi.org/10.1016/j.jbiotec.2010.09.947 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 150 IS - 3 SP - 408 EP - 416 PB - Elsevier CY - Amsterdam ER -