TY - CHAP A1 - Kasper, Katharina A1 - Schiffels, Johannes A1 - Krafft, Simone A1 - Kuperjans, Isabel A1 - Elbers, Gereon A1 - Selmer, Thorsten T1 - Biogas Production on Demand Regulated by Butyric Acid Addition T2 - IOP Conference Series: Earth and Environmental Science. Bd. 32 Y1 - 2016 U6 - https://doi.org/10.1088/1755-1315/32/1/012009 SN - 1755-1315 N1 - ICARET 2016, International Conference on Advances in Renewable Energy and Technologies, Putrajaya, MY, Feb 23-25, 2016 VL - 32 SP - 012009/1 EP - 012009/4 ER - TY - JOUR A1 - Kappler-Tanudyaya, Nathalie A1 - Schmitt, Heike A1 - Tippkötter, Nils A1 - Meyer, Lina A1 - Lenzen, Sigurd A1 - Ulber, Roland T1 - Combination of biotransformation and chromatography for the isolation and purification of mannoheptulose JF - Biotechnology Journal N2 - Mannoheptulose is a seven-carbon sugar. It is an inhibitor of glucose-induced insulin secretion due to its ability to selectively inhibit the enzyme glucokinase. An improved procedure for mannoheptulose isolation from avocados is described in this study (based upon the original method by La Forge). The study focuses on the combination of biotransformation and downstream processing (preparative chromatography) as an efficient method to produce a pure extract of mannoheptulose. The experiments were divided into two major phases. In the first phase, several methods and parameters were compared to optimize the mannoheptulose extraction with respect to efficiency and purity. In the second phase, a mass balance of mannoheptulose over the whole extraction process was undertaken to estimate the yield and efficiency of the total extraction process. The combination of biotransformation and preparative chromatography allowed the production of a pure mannoheptulose extract. In a biological test, the sugar inhibited the glucokinase enzyme activity efficiently. Y1 - 2007 U6 - https://doi.org/10.1002/biot.200700004 SN - 1860-7314 VL - 2 IS - 6 SP - 692 EP - 699 ER - TY - JOUR A1 - Kapelyukh, Yury A1 - Henderson, Colin James A1 - Scheer, Nico A1 - Rode, Anja A1 - Wolf, Charles Roland T1 - Defining the contribution of CYP1A1 and CYP1A2 to drug metabolism using humanized CYP1A1/1A2 and Cyp1a1/Cyp1a2 KO mice JF - Drug Metabolism and Disposition Y1 - 2019 U6 - https://doi.org/10.1124/dmd.119.087718 IS - Early view ER - TY - CHAP A1 - Kalbe, Jochen A1 - Kuropka, Rolf A1 - Meyer-Stork, L. Sebastian A1 - Lauter, S. L. A1 - Höcker, Hartwig A1 - Berndt, Heinz ED - Körner, Andrea T1 - Identification of fine animal hair via DNA analysis T2 - Proceedings of the 1st International Symposium on Specialty Animal Fibers : Aachen, October 26 - 27, 1987 ; [scientific, technological and economical aspects] . - (Schriftenreihe des Deutschen Wollforschungsinstitutes an der Technischen Hochschule Aachen e.V. ; 103) Y1 - 1988 SN - 0930-3723 SP - 221 EP - 227 PB - Dt. Wollforschungsinst. CY - Aachen ER - TY - JOUR A1 - Kalbe, Jochen A1 - Kuropka, Rolf A1 - Meyer-Stork, L. Sebastian A1 - Berndt, Heinz A1 - Sauter, Sybille L. A1 - Loss, Peter A1 - Hendo, Karsten A1 - Riesner, Detlev A1 - Höcker, Hartwig T1 - Isolation and characterization of high-molecular mass DNA from hair shafts JF - Biological chemistry Y1 - 1988 SN - 0177-3593 U6 - https://doi.org/10.1515/bchm3.1988.369.1.413 VL - 369 IS - 1 SP - 413 EP - 416 ER - TY - JOUR A1 - Kalbe, Jochen A1 - Höcker, Hartwig A1 - Berndt, Heinz T1 - Design of enzyme reactors as chromatographic columns for racemic resolution of amino acid esters JF - Chromatographia Y1 - 1989 SN - 0009-5893 U6 - https://doi.org/10.1007/BF02319646 VL - 28 IS - 3-4 SP - 193 EP - 196 ER - TY - JOUR A1 - Jossek, Ralf A1 - Bongaerts, Johannes A1 - Sprenger, Georg A. T1 - Characterization of a new feedback-resistant 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase AroF of Escherichia coli JF - FEMS microbiology letters Y1 - 2001 SN - 1574-6968 VL - Vol. 202 IS - Iss. 1 SP - 145 EP - 148 ER - TY - JOUR A1 - Jordan, Sabine D. A1 - Krüger, Markus A1 - Willmes, Diana M. A1 - Redemann, Nora A1 - Wunderlich, F. Thomas A1 - Brönneke, Hella S. A1 - Merkwirth, Carsten A1 - Kashkar, Hamid A1 - Olkkonen, Vesa M. A1 - Böttger, Thomas A1 - Braun, Thomas A1 - Seibler, Jost A1 - Brüning, Jens C. T1 - Obesity-induced overexpression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism JF - Nature Cell Biology N2 - The contribution of altered post-transcriptional gene silencing to the development of insulin resistance and type 2 diabetes mellitus so far remains elusive. Here, we demonstrate that expression of microRNA (miR)-143 and 145 is upregulated in the liver of genetic and dietary mouse models of obesity. Induced transgenic overexpression of miR-143, but not miR-145, impairs insulin-stimulated AKT activation and glucose homeostasis. Conversely, mice deficient for the miR-143–145 cluster are protected from the development of obesity-associated insulin resistance. Quantitative-mass-spectrometry-based analysis of hepatic protein expression in miR-143-overexpressing mice revealed miR-143-dependent downregulation of oxysterol-binding-protein-related protein (ORP) 8. Reduced ORP8 expression in cultured liver cells impairs the ability of insulin to induce AKT activation, revealing an ORP8-dependent mechanism of AKT regulation. Our experiments provide direct evidence that dysregulated post-transcriptional gene silencing contributes to the development of obesity-induced insulin resistance, and characterize the miR-143–ORP8 pathway as a potential target for the treatment of obesity-associated diabetes. Y1 - 2011 U6 - https://doi.org/10.1038/ncb2211 SN - 1465-7392 VL - 13 IS - 4 SP - 434 EP - 446 PB - Nature CY - New York ER - TY - JOUR A1 - Jeromin, Günter Erich A1 - Zoor, Annegreth T1 - A new irreversible enzyme-aided esterification method in organic solvents JF - Biotechnology letters. 30 (2008), H. 5 Y1 - 2008 SN - 1573-6776 SP - 925 EP - 928 ER - TY - JOUR A1 - Jeromin, Günter Erich A1 - Welsch, Volker T1 - Diketene a New Esterification Reagent in the Enzyme-Aided Synthesis of Chiral Alcohols and Chiral Acetoacetic Acid Esters JF - Tetrahedron Letters . 36 (1995), H. 37 Y1 - 1995 SN - 0040-4039 N1 - ISSN der E-Ausg.: 1359-8562 SP - 6663 EP - 6664 ER - TY - JOUR A1 - Jeromin, Günter Erich A1 - Scheidt, Annette T1 - Aliphatic optically active alcohols by enzyme-aided synthesis JF - Tetrahedron Letters . 32 (1991), H. 48 Y1 - 1991 SN - 0040-4039 N1 - ISSN der E-Ausg.: 1359-8562 SP - 7021 EP - 7024 ER - TY - JOUR A1 - Jeromin, Günter Erich A1 - Albertz, Michael T1 - Optically active α-acetoxycarboxylic acids and α-hydroxycarboxylic acids by enzyme-aided syntheses JF - Journal für Praktische Chemie / Chemiker-Zeitung. 334 (1992), H. 6 Y1 - 1992 SN - 1436-9966 N1 - ISSN der E-Ausg.: 1521-3897 ; In der Bibliothek (Jülich) vorhanden unter 63 ZS 024-1992 SP - 526 EP - 528 ER - TY - JOUR A1 - Jeromin, Günter Erich T1 - Superabsorbed alcohol dehydrogenase—a new catalyst for asymmetric reductions JF - Biotechnology Letters. 31 (2009), H. 11 Y1 - 2009 SN - 0141-5492 SP - 1717 EP - 1721 ER - TY - JOUR A1 - Jeromin, Günter Erich T1 - Tetrabenzo[a,c,h,j]phenoxazine-18-yl a new stable neutral radical JF - Tetrahedron Letters . 42 (2001), H. 10 Y1 - 2001 SN - 0040-4039 N1 - ISSN der E-Ausg.: 1359-8562 SP - 1863 EP - 1865 ER - TY - JOUR A1 - Jakob, Felix A1 - Martinez, Ronny A1 - Mandawe, John A1 - Hellmuth, Hendrik A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schwaneberg, Ulrich T1 - Surface charge engineering of a Bacillus gibsonii subtilisin protease JF - Applied microbiology and biotechnology Y1 - 2013 SN - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print) VL - Vol. 97 IS - Iss. 15 SP - 6793 EP - 6802 PB - Springer CY - Berlin ER - TY - JOUR A1 - Jahnke, J. A1 - Baumann, Marcus T1 - Differentiation between Phaeocystis pouchetii (Har.) Lagerheim and Phaeocystis globosa Scherffel JF - Hydrobiological bulletin Y1 - 1987 SN - 0165-1404 (Print); 1573-5125 (E-Journal) VL - Vol. 21 IS - Iss. 2 SP - 141 EP - 147 ER - TY - JOUR A1 - Jablonski, Melanie A1 - Münstermann, Felix A1 - Nork, Jasmina A1 - Molinnus, Denise A1 - Muschallik, Lukas A1 - Bongaerts, Johannes A1 - Wagner, Torsten A1 - Keusgen, Michael A1 - Siegert, Petra A1 - Schöning, Michael Josef T1 - Capacitive field‐effect biosensor applied for the detection of acetoin in alcoholic beverages and fermentation broths JF - physica status solidi (a) applications and materials science N2 - An acetoin biosensor based on a capacitive electrolyte–insulator–semiconductor (EIS) structure modified with the enzyme acetoin reductase, also known as butane-2,3-diol dehydrogenase (Bacillus clausii DSM 8716ᵀ), is applied for acetoin detection in beer, red wine, and fermentation broth samples for the first time. The EIS sensor consists of an Al/p-Si/SiO₂/Ta₂O₅ layer structure with immobilized acetoin reductase on top of the Ta₂O₅ transducer layer by means of crosslinking via glutaraldehyde. The unmodified and enzyme-modified sensors are electrochemically characterized by means of leakage current, capacitance–voltage, and constant capacitance methods, respectively. KW - acetoin KW - acetoin reductase KW - alcoholic beverages KW - biosensors KW - capacitive field-effect sensors Y1 - 2021 U6 - https://doi.org/10.1002/pssa.202000765 SN - 1862-6319 N1 - Corresponding author: Melanie Jablonski VL - 218 IS - 13 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Infantino, Angelo A1 - Paulßen, Elisabeth A1 - Mostacci, Domiziano A1 - Schaffer, Paul A1 - Trinczek, Michael A1 - Hoehr, Cornelia T1 - Assessment of the production of medical isotopes using the Monte Carlo code FLUKA: Simulations against experimental measurements JF - Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms N2 - The Monte Carlo code FLUKA is used to simulate the production of a number of positron emitting radionuclides, ¹⁸F, ¹³N, ⁹⁴Tc, ⁴⁴Sc, ⁶⁸Ga, ⁸⁶Y, ⁸⁹Zr, ⁵²Mn, ⁶¹Cu and ⁵⁵Co, on a small medical cyclotron with a proton beam energy of 13 MeV. Experimental data collected at the TR13 cyclotron at TRIUMF agree within a factor of 0.6 ± 0.4 with the directly simulated data, except for the production of ⁵⁵Co, where the simulation underestimates the experiment by a factor of 3.4 ± 0.4. The experimental data also agree within a factor of 0.8 ± 0.6 with the convolution of simulated proton fluence and cross sections from literature. Overall, this confirms the applicability of FLUKA to simulate radionuclide production at 13 MeV proton beam energy. Y1 - 2016 U6 - https://doi.org/10.1016/j.nimb.2015.10.067 SN - 1872-9584 VL - 366 SP - 117 EP - 123 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Inagaki, Akiko A1 - Sleddens-Linkels, Esther A1 - Wassenaar, Evelyne A1 - Ooms, Marja A1 - Cappellen, Wiggert A. van A1 - Hoeijmakers, Jan H. J. A1 - Seibler, Jost A1 - Vogt, Thomas F. A1 - Shin, Myung K. A1 - Grootegoed, J. Anton A1 - Baarends, Willy M. T1 - Meiotic functions of RAD18 JF - Journal of Cell Science Y1 - 2011 U6 - https://doi.org/10.1242/jcs.081968 SN - 1477-9137 VL - 124 IS - 16 SP - 2837 EP - 2850 PB - Company of Biologists Limited CY - Cambridge ER - TY - JOUR A1 - Immel, Timo A1 - Grützke, Martin A1 - Späte, Anne-Katrin A1 - Groth, Ulrich A1 - Öhlschläger, Peter A1 - Huhn, Thomas T1 - Synthesis and X-ray structure analysis of a heptacoordinate titanium(IV)-bis-chelate with enhanced in vivo antitumor efficacy JF - Chemical Communications N2 - Chelate stabilization of a titanium(IV)–salan alkoxide by ligand exchange with 2,6-pyridinedicarboxylic acid (dipic) resulted in heptacoordinate complex 3 which is not redox-active, stable on silica gel and has increased aqueous stability. 3 is highly toxic in HeLa S3 and Hep G2 and has enhanced antitumor efficacy in a mouse cervical-cancer model. Y1 - 2012 U6 - https://doi.org/10.1039/C2CC31624B SN - 1364-548X VL - 48 IS - 46 SP - 5790 EP - 5792 PB - Royal Society of Chemistry CY - Cambridge ER -