TY  - JOUR
A1  - Miyamoto, K.
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
A1  - Yoshinobu, T.
T1  - Multi-well structure for cell culture on the chemical imaging sensor
Y1  - 2011
N1  - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS) , Date: 5-9 June 2011
SP  - 2130
EP  - 2132
PB  - IEEE
CY  - New York
ER  - 
TY  - JOUR
A1  - Schubert, J.
A1  - Schöning, Michael Josef
A1  - Mourzina, Y. G.
A1  - Legin, A. V.
A1  - Vlasov, Y. G.
A1  - Zander, W.
A1  - Lüth, H.
T1  - Multicomponent thin films for electrochemical sensor applications prepared by pulsed laser deposition
JF  - Sensors and Actuators B. 76 (2001), H. 1-3
Y1  - 2001
SN  - 0925-4005
SP  - 327
EP  - 330
ER  - 
TY  - JOUR
A1  - Huck, Christina
A1  - Poghossian, Arshak
A1  - Kerroumi, Iman
A1  - Schusser, Sebastian
A1  - Bäcker, Matthias
A1  - Zander, Willi
A1  - Schubert, Jürgen
A1  - Buniatyan, Vahe V.
A1  - Martirosyan, Norayr W.
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Multiparameter sensor chip with Barium Strontium Titanate as multipurpose material
JF  - Electroanalysis
N2  - It is well known that biochemical and biotechnological processes are strongly dependent and affected by a variety of physico-chemical parameters such as pH value, temperature, pressure and electrolyte conductivity. Therefore, these quantities have to be monitored or controlled in order to guarantee a stable process operation, optimization and high yield. In this work, a sensor chip for the multiparameter detection of three physico-chemical parameters such as electrolyte conductivity, pH and temperature is realized using barium strontium titanate (BST) as multipurpose material. The chip integrates a capacitively coupled four-electrode electrolyte-conductivity sensor, a capacitive field-effect pH sensor and a thin-film Pt-temperature sensor. Due to the multifunctional properties of BST, it is utilized as final outermost coating layer of the processed sensor chip and serves as passivation and protection layer as well as pH-sensitive transducer material at the same time. The results of testing of the individual sensors of the developed multiparameter sensor chip are presented. In addition, a quasi-simultaneous multiparameter characterization of the sensor chip in buffer solutions with different pH value and electrolyte conductivity is performed. To study the sensor behavior and the suitability of BST as multifunctional material under harsh environmental conditions, the sensor chip was exemplarily tested in a biogas digestate.
Y1  - 2014
U6  - http://dx.doi.org/10.1002/elan.201400076
SN  - 1521-4109 (E-Journal); 1040-0397 (Print)
VL  - 26
IS  - 5
SP  - 980
EP  - 987
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Reisert, Steffen
A1  - Geissler, H.
A1  - Weiler, C.
A1  - Wagner, P.
A1  - Schöning, Michael Josef
T1  - Multiple sensor-type system for monitoring the microbicidal effectiveness of aseptic sterilisation processes
JF  - Food control
N2  - The present work describes a novel multiple sensor-type system for the real-time analysis of aseptic sterilisation processes employing gaseous hydrogen peroxide (H2O2) as a sterilant. The inactivation kinetics of Bacillus atrophaeus by gaseous H2O2 have been investigated by means of a methodical calibration experiment, taking into account the process variables H2O2 concentration, humidity and gas temperature. It has been found that the microbicidal effectiveness at H2O2 concentrations above 2% v/v is largely determined by the concentration itself, while at lower H2O2 concentrations, the gas temperature and humidity play a leading role. Furthermore, the responses of different types of gas sensors towards the influencing factors of the sterilisation process have been analysed within the same experiment. Based on a correlation established between the inactivation kinetics and the sensor responses, a calorimetric H2O2 sensor and a metal-oxide semiconductor (MOX) sensor have been identified as possible candidates for monitoring the microbicidal effectiveness of aseptic sterilisation processes employing gaseous H2O2. Therefore, two linear models that describe the relationship between sensor response and microbicidal effectiveness have been proposed.
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.foodcont.2014.07.063
SN  - 1873-7129 (E-Journal); 0956-7135 (Print)
VL  - 47
SP  - 615
EP  - 622
ER  - 
TY  - JOUR
A1  - Achtsnicht, Stefan
A1  - Pourshahidi, Ali Mohammad
A1  - Offenhäusser, Andreas
A1  - Krause, Hans-Joachim
T1  - Multiplex detection of different magnetic beads using frequency scanning in magnetic frequency mixing technique
JF  - Sensors
N2  - In modern bioanalytical methods, it is often desired to detect several targets in one sample within one measurement. Immunological methods including those that use superparamagnetic beads are an important group of techniques for these applications. The goal of this work is to investigate the feasibility of simultaneously detecting different superparamagnetic beads acting as markers using the magnetic frequency mixing technique. The frequency of the magnetic excitation field is scanned while the lower driving frequency is kept constant. Due to the particles’ nonlinear magnetization, mixing frequencies are generated. To record their amplitude and phase information, a direct digitization of the pickup-coil’s signal with subsequent Fast Fourier Transformation is performed. By synchronizing both magnetic beads using frequency scanning in magnetic frequency mixing technique magnetic fields, a stable phase information is gained. In this research, it is shown that the amplitude of the dominant mixing component is proportional to the amount of superparamagnetic beads inside a sample. Additionally, it is shown that the phase does not show this behaviour. Excitation frequency scans of different bead types were performed, showing different phases, without correlation to their diverse amplitudes. Two commercially available beads were selected and a determination of their amount in a mixture is performed as a demonstration for multiplex measurements.
KW  - frequency mixing magnetic detection
KW  - magnetic sandwich immunoassay
KW  - multiparametric immunoassays
Y1  - 2019
U6  - http://dx.doi.org/10.3390/s19112599
SN  - 1424-8220
VL  - 19
IS  - 11
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Pourshahidi, Ali Mohammad
A1  - Achtsnicht, Stefan
A1  - Nambipareechee, Mrinal Murali
A1  - Offenhäusser, Andreas
A1  - Krause, Hans-Joachim
T1  - Multiplex detection of magnetic beads using offset field dependent frequency mixing magnetic detection
JF  - Sensors
N2  - Magnetic immunoassays employing Frequency Mixing Magnetic Detection (FMMD) have recently become increasingly popular for quantitative detection of various analytes. Simultaneous analysis of a sample for two or more targets is desirable in order to reduce the sample amount, save consumables, and save time. We show that different types of magnetic beads can be distinguished according to their frequency mixing response to a two-frequency magnetic excitation at different static magnetic offset fields. We recorded the offset field dependent FMMD response of two different particle types at frequencies ƒ₁ + n⋅ƒ₂, n = 1, 2, 3, 4 with ƒ₁ = 30.8 kHz and ƒ₂ = 63 Hz. Their signals were clearly distinguishable by the locations of the extremes and zeros of their responses. Binary mixtures of the two particle types were prepared with different mixing ratios. The mixture samples were analyzed by determining the best linear combination of the two pure constituents that best resembled the measured signals of the mixtures. Using a quadratic programming algorithm, the mixing ratios could be determined with an accuracy of greater than 14%. If each particle type is functionalized with a different antibody, multiplex detection of two different analytes becomes feasible.
KW  - colorization
KW  - multiplex detection
KW  - frequency mixing magnetic detection
KW  - magnetic nanoparticles
Y1  - 2021
U6  - http://dx.doi.org/10.3390/s21175859
SN  - 1424-8220
N1  - This article belongs to the Special Issue "Advanced Nanomaterial-Based Sensors for Biomedical Applications"
VL  - 21
IS  - 17
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Karschuck, Tobias
A1  - Schmidt, Stefan
A1  - Achtsnicht, Stefan
A1  - Poghossian, Arshak
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Multiplexing system for automated characterization of a capacitive field-effect sensor array
JF  - Physica Status Solidi A
N2  - In comparison to single-analyte devices, multiplexed systems for a multianalyte detection offer a reduced assay time and sample volume, low cost, and high throughput. Herein, a multiplexing platform for an automated quasi-simultaneous characterization of multiple (up to 16) capacitive field-effect sensors by the capacitive–voltage (C–V) and the constant-capacitance (ConCap) mode is presented. The sensors are mounted in a newly designed multicell arrangement with one common reference electrode and are electrically connected to the impedance analyzer via the base station. A Python script for the automated characterization of the sensors executes the user-defined measurement protocol. The developed multiplexing system is tested for pH measurements and the label-free detection of ligand-stabilized, charged gold nanoparticles.
KW  - Capacitive field-effect sensor
KW  - Gold nanoparticles
KW  - Label-free detection
KW  - Multicell
KW  - Multiplexing
Y1  - 2023
U6  - http://dx.doi.org/10.1002/pssa.202300265
SN  - 1862-6300 (Print)
SN  - 1862-6319 (Online)
N1  - Corresponding author: Michael Josef Schöning
VL  - 220
IS  - 22
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Khaydukova, M. M.
A1  - Zadorozhnaya, O. A.
A1  - Kirsanov, D. O.
A1  - Iken, Heiko
A1  - Rolka, David
A1  - Schöning, Michael Josef
A1  - Babain, V. A.
A1  - Vlasov, Yu. G.
A1  - Legin, A. V.
T1  - Multivariate processing of atomic-force microscopy images for detection of the response of plasticized polymeric membranes
JF  - Russian journal of applied chemistry
N2  - The possibility of using the atomic-force microscopy as a method for detection of the analytical signal from plasticized polymeric sensor membranes was analyzed. The surfaces of cadmium-selective membranes based on two polymeric matrices were examined. The digital images were processed with multivariate image analysis techniques. A correlation was found between the surface profile of an ion-selective membrane and the concentration of the ion in solution.
Y1  - 2014
U6  - http://dx.doi.org/10.1134/S1070427214030112
SN  - 1608-3296 (E-Journal); 1070-4272 (Print)
VL  - 87
IS  - 3
SP  - 307
EP  - 314
PB  - Springer
CY  - Dordrecht
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Abouzar, Maryam H.
A1  - Christiaens, P.
A1  - Williams, O. A.
A1  - Haenen, K.
A1  - Wagner, P.
A1  - Schöning, Michael Josef
T1  - Nanocrystalline diamond-based field-effect (bio-)chemical sensor
JF  - 8. Dresdner Sensor-Symposium : Sensoren für Umwelt, Klima und Sicherheit, Biosensoren und Biosysteme, Sensoren und Sensorsysteme für die Prozesstechnik, Trends in der Sensortechnik, Materialentwicklung für die Sensorik; 8. Dresdner Sensor-Symposium, 10. - 12. Dezember 2007, Dresden / Gerald Gerlach ... (Hg.)
Y1  - 2007
SN  - 978-3-940046-45-1
N1  - Dresdner Sensor-Symposium <8, 2007, Dresden> ; Dresdner Beiträge zur Sensorik ; 29
SP  - 191
EP  - 194
PB  - TUDpress, Verl. der Wissenschaften
CY  - Dresden
ER  - 
TY  - JOUR
A1  - Christiaens, P.
A1  - Abouzar, Maryam H.
A1  - Poghossian, Arshak
A1  - Wagner, Torsten
A1  - Bijnens, N.
A1  - Williams, O. A.
A1  - Daenen, M.
A1  - Haenen, K.
A1  - Schöning, Michael Josef
A1  - Wagner, P.
T1  - Nanocrystalline diamond-based field-effect capacitive pH sensor
JF  - Transducers '07 Eurosensors XXI : digest of technical papers ; the14th International Conference on Solid-State Sensors, Actuators and Microsystems, June 10-14, 2007, Lyon, France / Gilles Delapierre (Ed.)
Y1  - 2007
SN  - 1-4244-0841-5
N1  - Eurosensors 21, 2007, Lyon ; International Conference on Solid-State Sensors, Actuators and Microsystems 14, 2007, Lyon
SP  - 1891
EP  - 1894
PB  - IEEE
CY  - Piscataway
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Abouzar, Maryam H.
A1  - Razavi, A.
A1  - Bäcker, Matthias
A1  - Bijnens, N.
A1  - Williams, O. A.
A1  - Haenen, K.
A1  - Moritz, W.
A1  - Wagner, P.
A1  - Schöning, Michael Josef
T1  - Nanocrystalline-diamond thin films with high pH and penicillin sensitivity prepared on a capacitive Si–SiO2 structure
JF  - Electrochimica Acta. 54 (2009), H. 25
Y1  - 2009
SN  - 0013-4686
SP  - 5981
EP  - 5985
ER  - 
TY  - JOUR
A1  - Morais, Paulo V.
A1  - Gomes, Vanderley F., Jr.
A1  - Silva, Anielle C. A.
A1  - Dantas, Noelio O.
A1  - Schöning, Michael Josef
A1  - Siqueira, José R., Jr.
T1  - Nanofilm of ZnO nanocrystals/carbon nanotubes as biocompatible layer for enzymatic biosensors in capacitive field-effect devices
JF  - Journal of Materials Science
N2  - The incorporation of nanomaterials that are biocompatible with different types of biological compounds has allowed the development of a new generation of biosensors applied especially in the biomedical field. In particular, the integration of film-based nanomaterials employed in field-effect devices can be interesting to develop biosensors with enhanced properties. In this paper, we studied the fabrication of sensitive nanofilms combining ZnO nanocrystals and carbon nanotubes (CNTs), prepared by means of the layer-by-layer (LbL) technique, in a capacitive electrolyte-insulator-semiconductor (EIS) structure for detecting glucose and urea. The ZnO nanocrystals were incorporated in a polymeric matrix of poly(allylamine) hydrochloride (PAH), and arranged with multi-walled CNTs in a LbL PAH-ZnO/CNTs film architecture onto EIS chips. The electrochemical characterizations were performed by capacitance–voltage and constant capacitance measurements, while the morphology of the films was characterized by atomic force microscopy. The enzymes glucose oxidase and urease were immobilized on film’s surface for detection of glucose and urea, respectively. In order to obtain glucose and urea biosensors with optimized amount of sensitive films, we investigated the ideal number of bilayers for each detection system. The glucose biosensor showed better sensitivity and output signal for an LbL PAH-ZnO/CNTs nanofilm with 10 bilayers. On the other hand, the urea biosensor presented enhanced properties even for the first bilayer, exhibiting high sensitivity and output signal. The presence of the LbL PAH-ZnO/CNTs films led to biosensors with better sensitivity and enhanced response signal, demonstrating that the adequate use of nanostructured films is feasible for proof-of-concept biosensors with improved properties that may be employed for biomedical applications.
Y1  - 2017
U6  - http://dx.doi.org/10.1007/s10853-017-1369-y
SN  - 1573-4803
VL  - 52
IS  - 20
SP  - 12314
EP  - 12325
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Abouzar, Maryam H.
A1  - Ingebrandt, S.
A1  - Poghossian, Arshak
A1  - Zhang, Y.
A1  - Vu, X. T.
A1  - Moritz, W.
A1  - Schöning, Michael Josef
T1  - Nanoplate field-effect capacitive (bio-)chemical sensor array based on SOI structure
JF  - Procedia Chemistry. 1 (2009), H. 1
Y1  - 2009
SN  - 1876-6196
N1  - Proceedings of the Eurosensors XXIII conference ; Eurosensors 23
SP  - 670
EP  - 673
ER  - 
TY  - CHAP
A1  - Poghossian, Arshak
A1  - Weiland, Maryam
A1  - Schöning, Michael Josef
ED  - Lvova, Larisa
ED  - Kirsanov, Dmitry
ED  - di Natale, Corrado
ED  - Legin, Audrey
T1  - Nanoplate field-effect capacitors: a new transducer structure for multiparameter (bio-)chemical sensing
T2  - Multisensor system for chemical analysis : materials and sensors
N2  - An array of electrically isolated nanoplate field-effect silicon-on-insulator (SOI) capacitors as a new transducer structure for multiparameter (bio-)chemical sensing is presented. The proposed approach allows addressable biasing and electrical readout of multiple nanoplate field-effect capacitive (bio-)chemical sensors on the same SOI chip, as well as differential-mode measurements. The realized sensor chip has been applied for pH and penicillin concentration measurements, electrical monitoring of polyelectrolyte multilayer formation, and the label-free electrical detection of consecutive deoxyribonucleic acid (DNA) hybridization and denaturation events.
Y1  - 2014
SN  - 978-981-4411-15-8 ; 978-981-4411-16-5
U6  - http://dx.doi.org/10.1201/b15491-11
SP  - 333
EP  - 373
PB  - Jenny Stanford Publishing
CY  - Singapore
ET  - 1
ER  - 
TY  - CHAP
A1  - Schöning, Michael Josef
A1  - Abdelghani, Adnane
T1  - Nanoscale Science and Technology (NS&T’12) : Proceedings Book Humboldt Kolleg <2012, Tunisia>  ; Tunisia, 17-19 March, 2012 / ed. by Michael J. Schöning ; Adnane Abdelghani
N2  - Proceedings of the 2nd Humboldt Kolleg, Hammamet, Tunisia Organizer: Alexander von Humboldt Stiftung, Germany. pdf 184 p. Welcome Address Dear Participants, Welcome to the 2nd Humboldt Kolleg in “Nanoscale Science and Technology” (NS&T’12) in Tunisia, sponsored by the "Alexander von Humboldt" foundation. The NS&T’12 multidisciplinary scientific program includes seven "hot" topics dealing with "Nanoscale Science and Technology" covering basic and application-oriented research as well as industrial (market) aspects: - Molecular Biophyics, Spectroscopy Techniques, Imaging Microscopy - Nanomaterials Synthesis for Medicine and Bio-chemical Sensors - Nanostructures, Semiconductors, Photonics and Nanodevices - New Technologies in Market Industry - Environment, Electro-chemistry, Bio-polymers and Fuel Cells - Nanomaterials, Photovoltaic, Modelling, Quantum Physics - Microelectronics, Sensors Networks and Embedded Systems We are deeply indebted to all members of the Scientific Committee and General Chairs for joint Sessions and to all speakers and chairmen, who have dedicated invaluable time and efforts for the realization of this event. On behalf of the Organizing Committee, we are cordially inviting you to join the conference and hope that your stay will be fruitful, rewarding and enjoyable. Prof. Dr. Michael J. Schöning, Prof. Dr. Adnane Abdelghani
KW  - Biosensor
KW  - Nanotechnologie
KW  - Nanomaterial
KW  - Nano Materials
KW  - Bio-Sensors
Y1  - 2012
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:a96-opus-3544
ER  - 
TY  - JOUR
A1  - Simonis, A.
A1  - Lüth, H.
A1  - Wang, J.
A1  - Schöning, Michael Josef
T1  - New concepts of miniaturised reference electrodes in silicon technology for potentiometric sensor systems
JF  - Sensors and Actuators B. 103 (2004), H. 1-2
Y1  - 2004
SN  - 0925-4005
SP  - 429
EP  - 435
ER  - 
TY  - JOUR
A1  - Falkenberg, Fabian
A1  - Voß, Leonie
A1  - Bott, Michael
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
T1  - New robust subtilisins from halotolerant and halophilic Bacillaceae
JF  - Applied Microbiology and Biotechnology
N2  - The aim of the present study was the characterisation of three true subtilisins and one phylogenetically intermediate subtilisin from halotolerant and halophilic microorganisms. Considering the currently growing enzyme market for efficient and novel biocatalysts, data mining is a promising source for novel, as yet uncharacterised enzymes, especially from halophilic or halotolerant Bacillaceae, which offer great potential to meet industrial needs. Both halophilic bacteria Pontibacillus marinus DSM 16465ᵀ and Alkalibacillus haloalkaliphilus DSM 5271ᵀ and both halotolerant bacteria Metabacillus indicus DSM 16189 and Litchfieldia alkalitelluris DSM 16976ᵀ served as a source for the four new subtilisins SPPM, SPAH, SPMI and SPLA. The protease genes were cloned and expressed in Bacillus subtilis DB104. Purification to apparent homogeneity was achieved by ethanol precipitation, desalting and ion-exchange chromatography. Enzyme activity could be observed between pH 5.0–12.0 with an optimum for SPPM, SPMI and SPLA around pH 9.0 and for SPAH at pH 10.0. The optimal temperature for SPMI and SPLA was 70 °C and for SPPM and SPAH 55 °C and 50 °C, respectively. All proteases showed high stability towards 5% (w/v) SDS and were active even at NaCl concentrations of 5 M. The four proteases demonstrate potential for future biotechnological applications.
KW  - Biotechnological application
KW  - Bacillaceae
KW  - Subtilisin
KW  - Subtilases
KW  - Halotolerant protease
Y1  - 2023
U6  - http://dx.doi.org/10.1007/s00253-023-12553-w
SN  - 1432-0614
N1  - Corresponding author: Petra Siegert
VL  - 107
SP  - 3939
EP  - 3954
PB  - Springer Nature
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Arida, Hassan A.
A1  - Al-haddad, Ameera
A1  - Schöning, Michael Josef
T1  - New Solid-State Organic Membrane Based Lead-Selective Micro-Electrode
JF  - International Journal of Electrochemical Science. 6 (2011), H. 9
Y1  - 2011
SN  - 1452-3981
SP  - 3858
EP  - 3867
ER  - 
TY  - JOUR
A1  - Karschuck, T. L.
A1  - Filipov, Y.
A1  - Bollella, P.
A1  - Schöning, Michael Josef
A1  - Katz, E.
T1  - Not-XOR (NXOR) logic gate based on an enzyme-catalyzed reaction
JF  - International Journal of Unconventional Computing
N2  - Enzyme-catalyzed reactions have been designed to mimic various Boolean logic gates in the general framework of unconventional biomolecular computing. While some of the logic gates, particularly OR, AND, are easy to realize with biocatalytic reactions and have been reported in numerous publications, some other, like NXOR, are very challenging and have not been realized yet with enzyme reactions. The paper reports on a novel approach to mimicking the NXOR logic gate using the bell-shaped enzyme activity dependent on pH values. Shifting pH from the optimum value to the acidic or basic values by using acid or base inputs (meaning 1,0 and 0,1 inputs) inhibits the enzyme reaction, while keeping the optimum pH (assuming 0,0 and 1,1 input combinations) preserves a high enzyme activity. The challenging part of the present approach is the selection of an enzyme with a well-demonstrated bell-shape activity dependence on the pH value. While many enzymes can satisfy this condition, we selected pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase as this enzyme has the optimum pH center-located on the pH scale allowing the enzyme activity change by the acidic and basic pH shift from the optimum value corresponding to the highest activity. The present NXOR gate is added to the biomolecular “toolbox” as a new example of Boolean logic gates based on enzyme reactions.
Y1  - 2019
SN  - 1548-7199
VL  - 14
IS  - 3-4
SP  - 235
EP  - 242
PB  - Old City Publishing
CY  - Philadelphia
ER  - 
TY  - JOUR
A1  - Haeger, Gerrit
A1  - Probst, Johanna
A1  - Jaeger, Karl-Erich
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
T1  - Novel aminoacylases from Streptomyces griseus DSM 40236 and their recombinant production in Streptomyces lividans
JF  - FEBS Open Bio
N2  - Amino acid-based surfactants are valuable compounds for cosmetic formulations. The chemical synthesis of acyl-amino acids is conventionally performed by the Schotten-Baumann reaction using fatty acyl chlorides, but aminoacylases have also been investigated for use in biocatalytic synthesis with free fatty acids. Aminoacylases and their properties are diverse; they belong to different peptidase families and show differences in substrate specificity and biocatalytic potential. Bacterial aminoacylases capable of synthesis have been isolated from Burkholderia, Mycolicibacterium, and Streptomyces. Although several proteases and peptidases from S. griseus have been described, no aminoacylases from this species have been identified yet. In this study, we investigated two novel enzymes produced by S. griseus DSM 40236ᵀ . We identified and cloned the respective genes and recombinantly expressed an α-aminoacylase (EC 3.5.1.14), designated SgAA, and an ε-lysine acylase (EC 3.5.1.17), designated SgELA, in S. lividans TK23. The purified aminoacylase SgAA was biochemically characterized, focusing on its hydrolytic activity to determine temperature- and pH optima and stabilities. The aminoacylase could hydrolyze various acetyl-amino acids at the Nα -position with a broad specificity regarding the sidechain. Substrates with longer acyl chains, like lauroyl-amino acids, were hydrolyzed to a lesser extent. Purified aminoacylase SgELA specific for the hydrolysis of Nε -acetyl-L-lysine was unstable and lost its enzymatic activity upon storage for a longer period but could initially be characterized. The pH optimum of SgELA was pH 8.0. While synthesis of acyl-amino acids was not observed with SgELA, SgAA catalyzed the synthesis of lauroyl-methionine.
KW  - Streptomyces lividans
KW  - recombinant expression
KW  - Streptomyces griseus
KW  - ε-lysine acylase
KW  - α-aminoacylase
Y1  - 2023
U6  - http://dx.doi.org/10.1002/2211-5463.13723
SN  - 2211-5463
N1  - Corresponding author: Petra Siegert
VL  - 13
IS  - 12
SP  - 2224
EP  - 2238
PB  - Wiley
CY  - Hoboken, NJ
ER  -