TY  - CHAP
A1  - Wu, Chunsheng
A1  - Bronder, Thomas
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
T1  - DNA-hybridization detection using light-addressable potentiometric sensor modified with gold layer
T2  - Sensoren und Messsysteme 2014 ; Beiträge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in Nürnberg. (ITG-Fachbericht ; 250)
Y1  - 2014
SN  - 978-3-8007-3622-5
SP  - 1
EP  - 4
PB  - VDE-Verl.
CY  - Düsseldorf
ER  - 
TY  - CHAP
A1  - Oberländer, Jan
A1  - Kirchner, Patrick
A1  - Keusgen, M.
A1  - Schöning, Michael Josef
T1  - Flexible polyimide-based calorimetric gas sensors for monitoring hy-drogen peroxide in sterilisation processes of aseptic filling machines
T2  - Sensoren und Messsysteme 2014 ; Beiträge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in Nürnberg. (ITG-Fachbericht ; 250)
Y1  - 2014
SN  - 978-3-8007-3622-5
SP  - 1
EP  - 4
PB  - VDE-Verl.
CY  - Düsseldorf
ER  - 
TY  - CHAP
A1  - Bäcker, Matthias
A1  - Schusser, Sebastian
A1  - Leinhos, Marcel
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
T1  - Sensor system for the monitoring of degradation processes of biodegradable biopolymers
T2  - Sensoren und Messsysteme 2014 ; Beiträge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in Nürnberg. (ITG-Fachbericht ; 250)
Y1  - 2014
SN  - 978-3-8007-3622-5
SP  - 1
EP  - 4
PB  - VDE-Verl.
CY  - Düsseldorf
ER  - 
TY  - CHAP
A1  - Voronkova, Eva B.
A1  - Bauer, Svetlana M.
A1  - Kotliar, Konstantin
T1  - Computer simulation of the cornea-scleral shell as applied to pressure-volume relationship in the human eye
T2  - 2014 International Conference on Computer Technologies in Physical and Engineering Applications :  ICCTPEA 2014 : proceedings : June 30 2014-July 4 2014, St. Petersburg
Y1  - 2014
SN  - 978-1-4799-5315-8
SP  - 204
EP  - 205
ER  - 
TY  - THES
A1  - Reyes Ochoa, Leonel
T1  - Engineering aspects of a parabolic trough collector field with direct steam generation and an organic rankine cycle
Y1  - 2014
N1  - Aachen, Fachhochsch., Masterarbeit, 2014
PB  - Fachhochschule Aachen
CY  - Aachen
ER  - 
TY  - JOUR
A1  - Almajhdi, Fahad N.
A1  - Senger, Tilo
A1  - Amer, Haitham M.
A1  - Gissmann, Lutz
A1  - Öhlschläger, Peter
T1  - Design of a highly effective therapeutic HPV16 E6/E7-specific DNA vaccine: optimization by different ways of sequence rearrangements (Shuffling)
JF  - PLOS one
N2  - Persistent infection with the high-risk Human Papillomavirus type 16 (HPV 16) is the causative event for the development of cervical cancer and other malignant tumors of the anogenital tract and of the head and neck. Despite many attempts to develop therapeutic vaccines no candidate has entered late clinical trials. An interesting approach is a DNA based vaccine encompassing the nucleotide sequence of the E6 and E7 viral oncoproteins. Because both proteins are consistently expressed in HPV infected cells they represent excellent targets for immune therapy. Here we report the development of 8 DNA vaccine candidates consisting of differently rearranged HPV-16 E6 and E7 sequences within one molecule providing all naturally occurring epitopes but supposedly lacking transforming activity. The HPV sequences were fused to the J-domain and the SV40 enhancer in order to increase immune responses. We demonstrate that one out of the 8 vaccine candidates induces very strong cellular E6- and E7- specific cellular immune responses in mice and, as shown in regression experiments, efficiently controls growth of HPV 16 positive syngeneic tumors. This data demonstrates the potential of this vaccine candidate to control persistent HPV 16 infection that may lead to malignant disease. It also suggests that different sequence rearrangements influence the immunogenecity by an as yet unknown mechanism.
Y1  - 2014
U6  - https://doi.org/10.1371/journal.pone.0113461
SN  - 1932-6203
VL  - 11
IS  - 9
PB  - PLOS
CY  - San Francisco
ER  - 
TY  - CHAP
A1  - Neumann, Tobias
A1  - Ferrein, Alexander
A1  - Kallweit, Stephan
A1  - Scholl, Ingrid
T1  - Towards a mobile mapping robot for underground mines
T2  - 7th Conference of Robotics and Mechatronics : RobMech 2014 : 27th and 28th Nov. 2014, Cape Town
Y1  - 2014
SP  - 1
EP  - 6
ER  - 
TY  - CHAP
A1  - Alhwarin, Faraj
A1  - Ferrein, Alexander
A1  - Scholl, Ingrid
T1  - IR stereo kinect: improving depth images by combining structured light with IR stereo
T2  - PRICAI 2014: Trends in artificial intelligence : 13th Pacific Rim International Conference on Artificial Intelligence : Gold Coast, QLD, Australia, December 1-5, 2014 : proceedings. (Lecture notes in computer science ; vol. 8862)
N2  - RGB-D sensors such as the Microsoft Kinect or the Asus Xtion are inexpensive 3D sensors. A depth image is computed by calculating the distortion of a known infrared light (IR) pattern which is projected into the scene. While these sensors are great devices they have some limitations. The distance they can measure is limited and they suffer from reflection problems on transparent, shiny, or very matte and absorbing objects. If more than one RGB-D camera is used the IR patterns interfere with each other. This results in a massive loss of depth information. In this paper, we present a simple and powerful method to overcome these problems. We propose a stereo RGB-D camera system which uses the pros of RGB-D cameras and combine them with the pros of stereo camera systems. The idea is to utilize the IR images of each two sensors as a stereo pair to generate a depth map. The IR patterns emitted by IR projectors are exploited here to enhance the dense stereo matching even if the observed objects or surfaces are texture-less or transparent. The resulting disparity map is then fused with the depth map offered by the RGB-D sensor to fill the regions and the holes that appear because of interference, or due to transparent or reflective objects. Our results show that the density of depth information is increased especially for transparent, shiny or matte objects.
Y1  - 2014
SN  - 978-3-319-13559-5 (Print) ; 978-3-319-13560-1 (E-Book)
U6  - https://doi.org/10.1007/978-3-319-13560-1_33
SP  - 409
EP  - 421
PB  - Springer
CY  - München
ER  - 
TY  - CHAP
A1  - Gebhardt, Andreas
A1  - Ritz, Thomas
A1  - Siekmann, Kirsten
A1  - Wallenborn, Ramona
ED  - Demmer, Axel
T1  - Additive manufacturing businesses in the process chain of individualized mass products
T2  - DDMC 2014 : Proceedings of the Fraunhofer Direct Digital Manufacturing Conference
Y1  - 2014
SN  - 978-3-8396-9128-1 (E-Book)
PB  - Fraunhofer
CY  - Stuttgart
ER  - 
TY  - CHAP
A1  - Ritz, Thomas
A1  - Izquierdo Tello, César
A1  - Damm, Sebastian
T1  - Connecting a pedelec to the cloud as basis for gamification in multi modal mobility planning
T2  - MobileCloud 2014 : 2nd IEEE International Conference on Mobile Cloud Computing, Services, and Engineering Oxford, United Kingdom 7-10 April 2014
Y1  - 2014
SN  - 978-1-4799-2504-9
U6  - https://doi.org/10.1109/MobileCloud.2014.25
SP  - 101
EP  - 108
PB  - IEEE Service Center
CY  - Piscataway, NJ
ER  - 
TY  - CHAP
A1  - Fateri, Miranda
A1  - Gebhardt, Andreas
T1  - Jewelry fabrication via selective laser melting of glass
T2  - ASME 2014 12th Biennial Conference on Engineering Systems Design and Analysis Volume 1: Applied Mechanics; Automotive Systems; Biomedical Biotechnology Engineering; Computational Mechanics; Design; Digital Manufacturing; Education; Marine and Aerospace Applications
N2  - Selective Laser Melting (SLM) is one of the Additive Manufacturing (AM) technologies applicable for producing complex geometries which are typically expensive or difficult to fabricate using conventional methods. This process has been extensively investigated experimentally for various metals and the fabrication process parameters have been established for different applications; however, fabricating 3D glass objects using SLM technology has remained a challenge so far although it could have many applications. This paper presents a summery on various experimental evaluations of a material database incorporating the build parameters of glass powder using the SLM process for jewelry applications.
Y1  - 2014
SN  - 978-0-7918-4583-7
U6  - https://doi.org/10.1115/ESDA2014-20380
SP  - V001T06A005
ER  - 
TY  - JOUR
A1  - Martin-Gonzalez, Anabel
A1  - Kotliar, Konstantin
A1  - Rios-Martinez, Jorge
A1  - Lanzl, Ines
A1  - Navab, Nassir
T1  - Mediated-reality magnification for macular degeneration rehabilitation
JF  - Journal of Modern Optics
Y1  - 2014
U6  - https://doi.org/10.1080/09500340.2014.936110
SN  - 1362-3044
VL  - 61
IS  - 17
SP  - 1400
EP  - 1408
PB  - Taylor & Francis
CY  - London
ER  - 
TY  - CHAP
A1  - Lenz, Laura L.
A1  - Wolf, Martin
ED  - Kritz, Willy Christian
T1  - Economic evaluation of serious games with the comparative assessment framework COSEGA
T2  - The shift from teaching to learning : individual, collective and organizational learning through gaming simulation ; proceedings of the 45th conference of the International Simulation and Gaming Association, Dornbirn 2014
Y1  - 2014
SN  - 978-3-7639-5422-3
SP  - 374
EP  - 386
PB  - Bertelsmann
CY  - [Bielefeld]
ER  - 
TY  - CHAP
A1  - Kirsch, Ansgar
A1  - Felber, W.
A1  - Marcher, Thomas
A1  - Fuchs, W.
T1  - Geotechnical challenges during design and construction of a ski resort in Azerbaijan
T2  - Geotechnics of roads and railways : proceedings of the 15th Danube - European Conference on Geotechnical Engineering : 9-11 September 2014, Vienna, Austria
Y1  - 2014
SN  - 978‐3‐902593‐01‐6
SP  - 757
EP  - 762
PB  - ÖIAV - Österreichischer Ingenieur- und Architekten-Verein
CY  - Wien
ER  - 
TY  - CHAP
A1  - Fuchs, Britta
A1  - Ritz, Thomas
T1  - Usability for Blended Shopping - Solving Major Flaws by Applying Usability Engineering and Proven Integration Technologies
T2  - Proceedings of the 11th International Conference on e-Business, ICE-B 2014, Vienna, Austria
Y1  - 2014
SN  - 978-989-758-043-7
U6  - https://doi.org/10.5220/0005113502160223
SP  - 216
EP  - 223
ER  - 
TY  - JOUR
A1  - Haag, S.
A1  - Zontar, D.
A1  - Schleupen, Josef
A1  - Müller, T.
A1  - Brecher, C.
T1  - Chain of refined perception in self-optimizing assembly of micro-optical systems
JF  - Journal of sensors and sensor systems
N2  - Today, the assembly of laser systems requires a large share of manual operations due to its complexity regarding the optimal alignment of optics. Although the feasibility of automated alignment of laser optics has been shown in research labs, the development effort for the automation of assembly does not meet economic requirements – especially for low-volume laser production. This paper presents a model-based and sensor-integrated assembly execution approach for flexible assembly cells consisting of a macro-positioner covering a large workspace and a compact micromanipulator with camera attached to the positioner. In order to make full use of available models from computer-aided design (CAD) and optical simulation, sensor systems at different levels of accuracy are used for matching perceived information with model data. This approach is named "chain of refined perception", and it allows for automated planning of complex assembly tasks along all major phases of assembly such as collision-free path planning, part feeding, and active and passive alignment. The focus of the paper is put on the in-process image-based metrology and information extraction used for identifying and calibrating local coordinate systems as well as the exploitation of that information for a part feeding process for micro-optics. Results will be presented regarding the processes of automated calibration of the robot camera as well as the local coordinate systems of part feeding area and robot base.
Y1  - 2014
U6  - https://doi.org/10.5194/jsss-3-87-2014
SN  - 2194-878X
VL  - 3
IS  - 1
SP  - 87
EP  - 95
PB  - Copernicus Publ.
CY  - Göttingen
ER  - 
TY  - CHAP
A1  - Heckl, O. H.
A1  - Weiler, Sascha
A1  - Fleischhaker, Robert
A1  - Gebs, Raphael
A1  - Budnicki, Aleksander
A1  - Wolf, M.
A1  - Kleinbauer, Jochen
A1  - Russ, S.
A1  - Kumkar, M.
A1  - Sutter, Dirk H.
ED  - Heisterkamp, Alexander
ED  - Herman, Peter R.
ED  - Meunier, Michael
ED  - Nolte, Stefan
T1  - Industry-grade high average power femtosecond light source
T2  - Frontiers in ultrafast optics: Biomedical, scientific, and industrial applications XIV : 1 Februar 2014, San Francisco, USA. - (SPIE proceedings series ; 8972)
Y1  - 2014
U6  - https://doi.org/10.1117/12.2039337
N1  - Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV <2, 2014, San Francisco, CA>
SP  - 89720P
EP  - 89720P-7
PB  - SPIE, International Society for Optical Engineering
ER  - 
TY  - JOUR
A1  - Scheer, Nico
A1  - Mclaughlin, Lesley A.
A1  - Rode, Anja
A1  - MacLeod, Alastair Kenneth
A1  - Henderson, Colin J.
A1  - Wolf, Roland C.
T1  - Deletion of thirty murine cytochrome P450 genes results in viable mice with compromised drug metabolism
JF  - Drug Metabolism and Disposition
N2  - In humans, 75% of all drugs are metabolized by the cytochrome P450-dependent monooxygenase system. Enzymes encoded by the CYP2C, CYP2D, and CYP3A gene clusters account for ∼80% of this activity. There are profound species differences in the multiplicity of cytochrome P450 enzymes, and the use of mouse models to predict pathways of drug metabolism is further complicated by overlapping substrate specificity between enzymes from different gene families. To establish the role of the hepatic and extrahepatic P450 system in drug and foreign chemical disposition, drug efficacy, and toxicity, we created a unique mouse model in which 30 cytochrome P450 genes from the Cyp2c, Cyp2d, and Cyp3a gene clusters have been deleted. Remarkably, despite a wide range of putative important endogenous functions, Cyp2c/2d/3a KO mice were viable and fertile, demonstrating that these genes have evolved primarily as detoxification enzymes. Although there was no overt phenotype, detailed examination showed Cyp2c/2d/3a KO mice had a smaller body size (15%) and larger livers (20%). Changes in hepatic morphology and a decreased blood glucose (30%) were also noted. A five-drug cocktail of cytochrome P450 isozyme probe substrates were used to evaluate changes in drug pharmacokinetics; marked changes were observed in either the pharmacokinetics or metabolites formed from Cyp2c, Cyp2d, and Cyp3a substrates, whereas the metabolism of the Cyp1a substrate caffeine was unchanged. Thus, Cyp2c/2d/3a KO mice provide a powerful model to study the in vivo role of the P450 system in drug metabolism and efficacy, as well as in chemical toxicity.
Y1  - 2014
U6  - https://doi.org/10.1124/dmd.114.057885
SN  - 1521-009X
VL  - 42
IS  - 6
SP  - 1022
EP  - 1030
PB  - ASPET
CY  - Bethesda, Md.
ER  - 
TY  - JOUR
A1  - Luisier, Raphaëlle
A1  - Lempiäinen, Harri
A1  - Scherbichler, Nina
A1  - Braeuning, Albert
A1  - Geissler, Miriam
A1  - Dubost, Valerie
A1  - Müller, Arne
A1  - Scheer, Nico
A1  - Chibout, Salah-Dine
A1  - Hara, Hisanori
A1  - Picard, Frank
A1  - Theil, Diethilde
A1  - Couttet, Philippe
A1  - Vitobello, Antonio
A1  - Grenet, Olivier
A1  - Grasl-Kraupp, Bettina
A1  - Ellinger-Ziegelbauer, Heidrung
A1  - Thomson, John P.
A1  - Meehan, Richard R.
A1  - Elcombe, Clifford R.
A1  - Henderson, Colin J.
A1  - Wolf, C. Roland
A1  - Schwarz, Michael
A1  - Moulin, Pierre
A1  - Terranova, Remi
A1  - Moggs, Jonathan G.
T1  - Phenobarbital Induces Cell Cycle Transcriptional Responses in Mouse Liver Humanized for Constitutive Androstane and Pregnane X Receptors
JF  - Toxicological Sciences
N2  - The constitutive androstane receptor (CAR) and the pregnane X receptor (PXR) are closely related nuclear receptors involved in drug metabolism and play important roles in the mechanism of phenobarbital (PB)-induced rodent nongenotoxic hepatocarcinogenesis. Here, we have used a humanized CAR/PXR mouse model to examine potential species differences in receptor-dependent mechanisms underlying liver tissue molecular responses to PB. Early and late transcriptomic responses to sustained PB exposure were investigated in liver tissue from double knock-out CAR and PXR (CARᴷᴼ-PXRᴷᴼ), double humanized CAR and PXR (CARʰ-PXRʰ), and wild-type C57BL/6 mice. Wild-type and CARʰ-PXRʰ mouse livers exhibited temporally and quantitatively similar transcriptional responses during 91 days of PB exposure including the sustained induction of the xenobiotic response gene Cyp2b10, the Wnt signaling inhibitor Wisp1, and noncoding RNA biomarkers from the Dlk1-Dio3 locus. Transient induction of DNA replication (Hells, Mcm6, and Esco2) and mitotic genes (Ccnb2, Cdc20, and Cdk1) and the proliferation-related nuclear antigen Mki67 were observed with peak expression occurring between 1 and 7 days PB exposure. All these transcriptional responses were absent in CARᴷᴼ-PXRᴷᴼ mouse livers and largely reversible in wild-type and CARʰ-PXRʰ mouse livers following 91 days of PB exposure and a subsequent 4-week recovery period. Furthermore, PB-mediated upregulation of the noncoding RNA Meg3, which has recently been associated with cellular pluripotency, exhibited a similar dose response and perivenous hepatocyte-specific localization in both wild-type and CARʰ-PXRʰ mice. Thus, mouse livers coexpressing human CAR and PXR support both the xenobiotic metabolizing and the proliferative transcriptional responses following exposure to PB.
Y1  - 2014
U6  - https://doi.org/https://doi.org/10.1093/toxsci/kfu038
SN  - 1094-2025
VL  - 139
IS  - 2
SP  - 501
EP  - 511
PB  - Oxford University Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Salpati, Laurent
A1  - Chu, Xiaoyan
A1  - Chen, Liangfu
A1  - Prasad, Bhagwat
A1  - Dallas, Shannon
A1  - Evers, Raymond
A1  - Mamaril-Fishman, Donna
A1  - Geier, Ethan G.
A1  - Kehler, Jonathan
A1  - Kunta, Jeevan
A1  - Mezler, Mario
A1  - Laplanche, Loic
A1  - Pang, Jodie
A1  - Soars, Matthew G.
A1  - Unadkat, Jashvant D.
A1  - van Waterschoot, Robert A.B.
A1  - Yabut, Jocelyn
A1  - Schinkel, Alfred H.
A1  - Scheer, Nico
A1  - Rode, Anja
T1  - Evaluation of organic anion transporting polypeptide 1B1 and 1B3 humanized mice as a translational model to study the pharmacokinetics of statins
JF  - Drug Metabolism and Disposition
N2  - Organic anion transporting polypeptide (Oatp) 1a/1b knockout and OATP1B1 and -1B3 humanized mouse models are promising tools for studying the roles of these transporters in drug disposition. Detailed characterization of these models will help to better understand their utility for predicting clinical outcomes. To advance this approach, we carried out a comprehensive analysis of these mouse lines by evaluating the compensatory changes in mRNA expression, quantifying the amounts of OATP1B1 and -1B3 protein by liquid chromatography–tandem mass spectrometry, and studying the active uptake in isolated hepatocytes and the pharmacokinetics of some prototypical substrates including statins. Major outcomes from these studies were 1) mostly moderate compensatory changes in only a few genes involved in drug metabolism and disposition, 2) a robust hepatic expression of OATP1B1 and -1B3 proteins in the respective humanized mouse models, and 3) functional activities of the human transporters in hepatocytes isolated from the humanized models with several substrates tested in vitro and with pravastatin in vivo. However, the expression of OATP1B1 and -1B3 in the humanized models did not significantly alter liver or plasma concentrations of rosuvastatin and pitavastatin compared with Oatp1a/1b knockout controls under the conditions used in our studies. Hence, although the humanized OATP1B1 and -1B3 mice showed in vitro and/or in vivo functional activity with some statins, further characterization of these models is required to define their potential use and limitations in the prediction of drug disposition and drug-drug interactions in humans.
Y1  - 2014
U6  - https://doi.org/10.1124/dmd.114.057976
SN  - 1521-009X
VL  - 42
IS  - 8
SP  - 1301
EP  - 1313
PB  - ASPET
CY  - Bethesda, Md.
ER  -