TY  - JOUR
A1  - Achtsnicht, Stefan
A1  - Neuendorf, Christian
A1  - Faßbender, Tobias
A1  - Nölke, Greta
A1  - Offenhäusser, Andreas
A1  - Krause, Hans-Joachim
A1  - Schröper, Florian
T1  - Sensitive and rapid detection of cholera toxin subunit B using magnetic frequency mixing detection
JF  - Plos One
N2  - Cholera is a life-threatening disease caused by the cholera toxin (CT) as produced by some Vibrio cholerae serogroups. In this research we present a method which directly detects the toxin’s B subunit (CTB) in drinking water. For this purpose we performed a magnetic sandwich immunoassay inside a 3D immunofiltration column. We used two different commercially available antibodies to capture CTB and for binding to superparamagnetic beads. ELISA experiments were performed to select the antibody combination. The beads act as labels for the magnetic frequency mixing detection technique. We show that the limit of detection depends on the type of magnetic beads. A nonlinear Hill curve was fitted to the calibration measurements by means of a custom-written python software. We achieved a sensitive and rapid detection of CTB within a broad concentration range from 0.2 ng/ml to more
than 700 ng/ml.
Y1  - 2019
U6  - http://dx.doi.org/10.1371/journal.pone.0219356
SN  - 1932-6203
VL  - 14
IS  - 7
PB  - Plos
CY  - San Francisco
ER  - 
TY  - CHAP
A1  - Abbas, Karim
A1  - Thurn, Laura
A1  - Kessler, Julia
A1  - Eichler, Fabian
T1  - Basic research of the consideration of additive manufactured lattice structures under thermoand fluid dynamic loads
T2  - Modern technologies in manufacturing (MTeM 2019)
Y1  - 2019
U6  - http://dx.doi.org/10.1051/matecconf/201929901009
N1  - MATEC Web of Conferences 299; MTeM 2019
VL  - 299
IS  - Article 01009
ER  -