TY  - JOUR
A1  - Öhlschläger, Peter
A1  - Osen, Wolfram
A1  - Peiler, Tanja
A1  - Caldeira, Sandra
T1  - A DNA vaccine based on a shuffled E7 oncogene of the human papillomavirus type 16 (HPV 16) induces E7-specific cytotoxic T cells but lacks transforming activity / Osen, Wolfram ; Peiler, Tanja ; Öhlschläger, Peter ; Caldeira, Sandra ; Faath, Stefan ; Mich
JF  - Vaccine. 19 (2001), H. 20
Y1  - 2001
SN  - 0264-410X
SP  - 4276
EP  - 4286
ER  - 
TY  - JOUR
A1  - Schiffels, Johannes
A1  - Selmer, Thorsten
T1  - A flexible toolbox to study protein-assisted metalloenzyme assembly in vitro
JF  - Biotechnology and Bioengineering
Y1  - 2015
U6  - http://dx.doi.org/10.1002/bit.25658
SN  - 1097-0290
VL  - 112
IS  - 11
SP  - 2360
EP  - 2372
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Bouwman, Peter
A1  - Gulden, Hanneke van der
A1  - Heijden, Ingrid van der
A1  - Drost, Rinske
A1  - Klijn, Christiaan N.
A1  - Prasetyanti, Pramudita
A1  - Pieterse, Mark
A1  - Wientjens, Ellen
A1  - Seibler, Jost
A1  - Hogervorst, Frank B. L.
A1  - Jonkers, Jos
T1  - A High-Throughput Functional Complementation Assay for Classification of BRCA1 Missense Variants
JF  - Cancer Discovery
Y1  - 2013
U6  - http://dx.doi.org/10.1158/2159-8290.CD-13-0094
SN  - 2159-8290
IS  - 3
SP  - 1142
EP  - 1152
ER  - 
TY  - JOUR
A1  - Aletsee, Ludwig
A1  - Baumann, Marcus
T1  - A laboratory incubator equipped with facilities to automatically simulate natural irradiance
JF  - Boletim do Instituto Oceanográfico / Universidade de São Paulo
Y1  - 1991
SN  - 1982-436X; 0080-6331
VL  - Vol. 39
IS  - No. 2
SP  - 155
EP  - 159
ER  - 
TY  - JOUR
A1  - Wagner, Torsten
A1  - Molina, R.
A1  - Biselli, Manfred
A1  - Canzoneri, Michele
A1  - Schnitzler, Thomas
A1  - Yoshinobu, Tatsuo
A1  - Schöning, Michael Josef
T1  - A light-addressable potentiometric sensor system for fast, simultaneous and spatial detection of the metabolic activity of biological cells
JF  - Transducers '07 Eurosensors XXI : digest of technical papers ; the14th International Conference on Solid-State Sensors, Actuators and Microsystems, June 10-14, 2007, Lyon, France / Gilles Delapierre (Ed.)
Y1  - 2007
SN  - 1-4244-0841-5
N1  - Eurosensors 21, 2007, Lyon ; International Conference on Solid-State Sensors, Actuators and Microsystems 14, 2007, Lyon
SP  - 1107
EP  - 1110
PB  - IEEE
CY  - Piscataway
ER  - 
TY  - JOUR
A1  - Mayer, Jan
A1  - Hentschke, Reinhard
A1  - Hager, Jonathan
A1  - Hojdis, Nils
A1  - Karimi-Varnaneh, Hossein Ali
T1  - A Nano-Mechanical Instability as Primary Contribution to Rolling Resistance
JF  - Scientific Reports
Y1  - 2017
SN  - 2045-2322
VL  - 7
IS  - Article number 11275
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Schmidt, S.
A1  - Büchs, J.
A1  - Born, C.
T1  - A new correlation for the wall-to-fluid mass transfer in liquid-sol fluidized beds / Schmidt, S. ; Büchs, J. ; Born, C. ; Biselli, M.
JF  - Chemical Engineering Science. 54 (1999), H. 6
Y1  - 1999
SN  - 0009-2509
SP  - 829
EP  - 839
ER  - 
TY  - JOUR
A1  - Jeromin, Günter Erich
A1  - Zoor, Annegreth
T1  - A new irreversible enzyme-aided esterification method in organic solvents
JF  - Biotechnology letters. 30 (2008), H. 5
Y1  - 2008
SN  - 1573-6776
SP  - 925
EP  - 928
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Schmidt, Bernhard
A1  - Ingendoh, Arnd
A1  - Figura, Kurt von
T1  - A novel amino acid modification in sulfatases that is defective in multiple sulfatase deficiency / Schmidt, Bernhard ; Selmer, Thorsten ;  Ingendoh, Arnd ;  Figurat, Kurt von
JF  - Cell. 82 (1995), H. 2
Y1  - 1995
SN  - 0092-8674
SP  - 271
EP  - 278
ER  - 
TY  - JOUR
A1  - Thiel, Alexander
A1  - Muffler, Kai
A1  - Tippkötter, Nils
A1  - Suck, Kirstin
A1  - Sohling, Ulrich
A1  - Hruschka, Steffen M.
A1  - Ulber, Roland
T1  - A novel integrated downstream processing approach to recover sinapic acid, phytic acid and proteins from rapeseed meal
JF  - Journal of Chemical Technology and Biotechnology
N2  - BACKGROUND
Currently, several techniques exist for the downstream processing of protein, phytic acid and sinapic acid from rapeseed and rapeseed meal, but no technique has been developed to separate all of the components in one process. In this work, two new downstream processing strategies focusing on recovering sinapic acid, phytic acid and protein from rapeseed meal were established.

RESULTS
The sinapic acid content was enhanced by a factor of 4.5 with one method and 5.1 with the other. The isolation of sinapic acid was accomplished using a zeolite-based adsorbent with high adsorptive and optimal desorption characteristics. Phytic acid was isolated using the anion-exchange resin Purolite A200®. In addition, the processes resulted in two separated protein fractions. The ratios of globulin and albumin ratio to the total protein were 59.2% and 40.1%, respectively. The steps were then combined in two different ways: (a) a ‘sequential process’ using the zeolite and A200 in batch processes; and (b) a ‘parallel process’ using only A200 in a chromatographic system to separate all of the compounds.

CONCLUSIONS
It can be concluded that isolation of all three components was possible in both processes. These could enhance the added value of current processes using rapeseed meal as a protein source. © 2015 Society of Chemical Industry
Y1  - 2015
U6  - http://dx.doi.org/10.1002/jctb.4664
VL  - 90
IS  - 11
SP  - 1999
EP  - 2006
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Scheer, Nico
A1  - Ross, Jillian
A1  - Rode, Anja
A1  - Zevnik, Branko
A1  - Niehaves, Sandra
A1  - Faust, Nicole
A1  - Wolf, C. Roland
T1  - A novel panel of mouse models to evaluate the role of human pregnane X receptor and constitutive androstane receptor in drug response
JF  - Journal of Clinical Investigation
Y1  - 2008
U6  - http://dx.doi.org/https://doi.org/10.1172/JCI35483
SN  - 1558-8238
VL  - 118
IS  - 9
SP  - 3228
EP  - 3239
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Figura, Kurt von
A1  - Schmidt, Bernhard
A1  - Dierks, T.
T1  - A novel protein modification generating an aldehyde group in sulfatases: its role in catalysis and disease / Figura, Kurt von ; Schmidt, Bernhard ; Selmer, Thorsten ; Dierks, Thomas
JF  - Bioessays. 20 (1998), H. 6
Y1  - 1998
SN  - 1521-1878
SP  - 505
EP  - 510
ER  - 
TY  - JOUR
A1  - Scheer, Nico
A1  - Riedl, Iris
A1  - Warren, J.T.
A1  - Kuwada, John Y.
A1  - Campos-Ortega, José A.
T1  - A quantitative analysis of the kinetics of Gal4 activator and effector gene expression in the zebrafish
JF  - Mechanism of Development
Y1  - 2002
U6  - http://dx.doi.org/10.1016/S0925-4773(01)00621-9
SN  - 0925-4773
VL  - 112
IS  - 1-2
SP  - 9
EP  - 14
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Stückmann, Henning
A1  - Kroll, Stephen
A1  - Winkelmann, Gunda
A1  - Noack, Udo
A1  - Scheper, Thomas
A1  - Ulber, Roland
T1  - A semi-quantitative dipstick assay for microcystin
JF  - Analytical and Bioanalytical Chemistry
N2  - An immunochromatographic lateral flow dipstick assay for the fast detection of microcystin-LR was developed. Colloid gold particles with diameters of 40 nm were used as red-colored antibody labels for the visual detection of the antigen. The new dipstick sensor is capable of detecting down to 5 µg·l−1 (ppb; total inversion of the color signal) or 1 ppb (observation of color grading) of microcystin-LR. The course of the labeling reaction was observed via spectrometric wave shifts caused by the change of particle size during the binding of antibodies. Different stabilizing reagents showed that especially bovine serum albumin (BSA) and casein increase the assays sensitivity and the conjugate stability. Performance of the dipsticks was quantified by pattern processing of capture zone CCD images. Storage stability of dipsticks and conjugate suspensions over 115 days under different conditions were monitored. The ready-to-use dipsticks were successfully tested with microcystin-LR-spiked samples of outdoor drinking- and salt water and applied to the tissue of microcystin-fed mussels.
Y1  - 2009
U6  - http://dx.doi.org/10.1007/s00216-009-2750-8
SN  - 1618-2650
VL  - 394
IS  - 3
SP  - 863
EP  - 869
PB  - springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Winckler, Silvia
A1  - Krueger, Rolf
A1  - Schnitzler, Thomas
A1  - Zang, Werner
A1  - Fischer, Rainer
A1  - Biselli, Manfred
T1  - A sensitive monitoring system for mammalian cell cultivation processes: a PAT approach
JF  - Bioprocess and biosystems engineering
N2  - Biopharmaceuticals such as antibodies are produced in cultivated mammalian cells, which must be monitored to comply with good manufacturing practice. We, therefore, developed a fully automated system comprising a specific exhaust gas analyzer, inline analytics and a corresponding algorithm to precisely determine the oxygen uptake rate, carbon dioxide evolution rate, carbon dioxide transfer rate, transfer quotient and respiratory quotient without interrupting the ongoing cultivation, in order to assess its reproducibility. The system was verified using chemical simulation experiments and was able to measure the respiratory activity of hybridoma cells and DG44 cells (derived from Chinese hamster ovary cells) with satisfactory results at a minimum viable cell density of ~2.0 × 10⁵ cells ml⁻¹. The system was suitable for both batch and fed-batch cultivations in bubble-aerated and membrane-aerated reactors, with and without the control of pH and dissolved oxygen.
Y1  - 2014
U6  - http://dx.doi.org/10.1007/s00449-013-1062-8
SN  - 1615-7591 (Print) 1615-7605 (Online)
VL  - 37
IS  - 5
SP  - 901
EP  - 912
PB  - Springer
CY  - Berlin, Heidelberg
ER  - 
TY  - JOUR
A1  - Monakhova, Yulia
A1  - Diehl, Bernd W. K.
T1  - A step towards optimization of the qNMR workflow: proficiency testing exercise at an GxP-accredited laboratory
JF  - Applied Magnetic Resonance
N2  - Quantitative nuclear magnetic resonance (qNMR) is considered as a powerful tool for multicomponent mixture analysis as well as for the purity determination of single compounds. Special attention is currently paid to the training of operators and study directors involved in qNMR testing. To assure that only qualified personnel are used for sample preparation at our GxP-accredited laboratory, weighing test was proposed. Sixteen participants performed six-fold weighing of the binary mixture of dibutylated hydroxytoluene (BHT) and 1,2,4,5-tetrachloro-3-nitrobenzene (TCNB). To evaluate the quality of data analysis, all spectra were evaluated manually by a qNMR expert and using in-house developed automated routine. The results revealed that mean values are comparable and both evaluation approaches are free of systematic error. However, automated evaluation resulted in an approximately 20% increase in precision. The same findings were revealed for qNMR analysis of 32 compounds used in pharmaceutical industry. Weighing test by six-fold determination in binary mixtures and automated qNMR methodology can be recommended as efficient tools for evaluating staff proficiency. The automated qNMR method significantly increases throughput and precision of qNMR for routine measurements and extends application scope of qNMR.
Y1  - 2021
U6  - http://dx.doi.org/10.1007/s00723-021-01324-3
SN  - 1613-7507
N1  - Corresponding author: Yulia Monakhova
VL  - 52
SP  - 581
EP  - 593
PB  - Springer Nature
CY  - Wien
ER  - 
TY  - JOUR
A1  - Wissenbach, U.
A1  - Six, S.
A1  - Bongaerts, Johannes
A1  - Ternes, D.
A1  - Steinwachs, S.
A1  - Unden, G.
T1  - A third periplasmic transport system for l-arginine in Escherichia coli: molecular characterization of the artPIQMJ genes, arginine binding and transport
JF  - Molecular microbiology
Y1  - 1995
SN  - 1365-2958 (E-Journal); 0950-382x (Print)
VL  - Vol. 17
IS  - Iss. 4
SP  - 675
EP  - 686
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Thamer, Wiebke
A1  - Cirpus, Irina
A1  - Hans, Marcus
T1  - A two [4Fe-4S]-cluster-containing ferredoxin as an alternative electron donor for 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans / Thamer, Wiebke ; Cirpus, Irina ; Hans, Marcus ; Pierik, Antonio, J. ; Selmer, Thorsten ; Bill, Eckhard ;
JF  - Archives of Microbiology. 179 (2003), H. 3
Y1  - 2003
SN  - 1432-072X
SP  - 197
EP  - 204
ER  - 
TY  - JOUR
A1  - Mang, Thomas
T1  - Abdichtmaterialien gegen drückendes Wasser
JF  - Bauchemie von der Forschung bis zur Praxis : 3. Tagung Bauchemie in Würzburg ; 27./28. September 2001 in Würzburg / GDCh-Fachgruppe Bauchemie ; [Red.: W. Hiller ...]
Y1  - 2002
SN  - 3-936028-04-4
N1  - Tagung Bauchemie <3, 2001, Würzburg> GdCh-Monographie 24 ; Monographie / GDCh, Gesellschaft Deutscher Chemiker ; 24
SP  - 65
PB  - GDCh
CY  - Frankfurt am Main
ER  - 
TY  - JOUR
A1  - Mang, Thomas
A1  - Haulena, Friedhelm
T1  - Abdichtmaterialien gegen drückendes Wasser und Diesel mit verbesserter Umweltverträglichkeit
JF  - Internationale Zeitschrift für Bauinstandsetzen und Baudenkmalpflege = International journal for restoration of buildings and monuments. 5 (1999), H. 1
Y1  - 1999
SN  - 0947-4498
SP  - 57
ER  -