TY - JOUR A1 - Pasteur, Aline A1 - Tippkötter, Nils A1 - Kampeis, Percy A1 - Ulber, Roland T1 - Optimization of high gradient magnetic separation filter units for the purification of fermentation products JF - IEEE TRANSACTIONS ON MAGNETICS N2 - High gradient magnetic separation (HGMS) has been established since the early 1970s. A more recent application of these systems is the use in bioprocesses. To integrate the HGMS in a fermentation process, it is necessary to optimize the separation matrix with regard to the magnetic separation characteristics and permeability of the non-magnetizable components of the fermentation broth. As part of the work presented here, a combined fluidic and magnetic force finite element model simulation was created using the software COMSOL Multiphysics and compared with separation experiments. Finally, as optimal lattice orientation of the separation matrix, a transversal rhombohedral arrangement was defined. The high suitability of the new filter matrix has been verified by separation experiments. Y1 - 2014 U6 - http://dx.doi.org/10.1109/TMAG.2014.2325535 SN - 0018-9464 N1 - Article Sequence Number: 5000607 INSPEC Accession Number: 14663042 VL - 50 IS - 10 SP - Artikel 5000607 PB - IEEE CY - New York, NY ER - TY - JOUR A1 - Degering, Christian A1 - Eggert, Thorsten A1 - Puls, Michael A1 - Bongaerts, Johannes A1 - Evers, Stefan A1 - Maurer, Karl-Heinz A1 - Jaeger, Karl-Erich T1 - Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides JF - Applied and environmental microbiology N2 - Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains. Y1 - 2010 U6 - http://dx.doi.org/10.1128/AEM.01146-10 SN - 1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print) VL - 76 IS - 19 SP - 6370 EP - 6378 PB - American Society for Microbiology CY - Washington, DC ER - TY - JOUR A1 - Hoffstadt, Kevin A1 - Nikolausz, Marcell A1 - Krafft, Simone A1 - Bonatelli, Maria A1 - Kumar, Vivekanantha A1 - Harms, Hauke A1 - Kuperjans, Isabel T1 - Optimization of the ex situ biomethanation of hydrogen and carbon dioxide in a novel meandering plug flow reactor: start-up phase and flexible operation JF - Bioengineering KW - methanation KW - plug flow reactor KW - bubble column KW - biomethane KW - P2G Y1 - 2024 U6 - http://dx.doi.org/10.3390/bioengineering11020165 SN - 2306-5354 VL - 11 IS - 2 PB - MDPI CY - Basel ER - TY - JOUR A1 - Penner, Crystal A1 - Usherovich, Samuel A1 - Niedermeier, Jana A1 - Bélanger-Champagne, Camille A1 - Trinczek, Michael A1 - Paulßen, Elisabeth A1 - Hoehr, Cornelia T1 - Organic Scintillator-Fibre Sensors for Proton Therapy Dosimetry: SCSF-3HF and EJ-260 JF - electronics N2 - In proton therapy, the dose from secondary neutrons to the patient can contribute to side effects and the creation of secondary cancer. A simple and fast detection system to distinguish between dose from protons and neutrons both in pretreatment verification as well as potentially in vivo monitoring is needed to minimize dose from secondary neutrons. Two 3 mm long, 1 mm diameter organic scintillators were tested for candidacy to be used in a proton–neutron discrimination detector. The SCSF-3HF (1500) scintillating fibre (Kuraray Co. Chiyoda-ku, Tokyo, Japan) and EJ-260 plastic scintillator (Eljen Technology, Sweetwater, TX, USA) were irradiated at the TRIUMF Neutron Facility and the Proton Therapy Research Centre. In the proton beam, we compared the raw Bragg peak and spread-out Bragg peak response to the industry standard Markus chamber detector. Both scintillator sensors exhibited quenching at high LET in the Bragg peak, presenting a peak-to-entrance ratio of 2.59 for the EJ-260 and 2.63 for the SCSF-3HF fibre, compared to 3.70 for the Markus chamber. The SCSF-3HF sensor demonstrated 1.3 times the sensitivity to protons and 3 times the sensitivity to neutrons as compared to the EJ-260 sensor. Combined with our equations relating neutron and proton contributions to dose during proton irradiations, and the application of Birks’ quenching correction, these fibres provide valid candidates for inexpensive and replicable proton-neutron discrimination detectors Y1 - 2022 U6 - http://dx.doi.org/10.3390/electronics12010011 SN - 2079-9292 N1 - This article belongs to the Special Issue "Applications of Optical Fiber Sensors" VL - 12 IS - 1 PB - MDPI CY - Basel ER - TY - JOUR A1 - Selmer, Thorsten A1 - Buckel, Wolfgang T1 - Oxygen Exchange between Acetate and the Catalytic Glutamate Residue in Glutaconate CoA-transferase from Acidaminococcus fermentans. IMPLICATIONS FOR THE MECHANISM OF CoA-ESTER HYDROLYSIS JF - Journal of Biological Chemistry. 274 (1999), H. 30 Y1 - 1999 SN - 1083-351X SP - 20772 EP - 20778 ER - TY - JOUR A1 - Unden, G. A1 - Becker, S. A1 - Bongaerts, Johannes A1 - Schirawski, J. A1 - Six, S. T1 - Oxygen regulated gene expression in facultatively anaerobic bacteria JF - Antonie van Leeuwenhoek Y1 - 1994 SN - 0003-6072 (Print) ; 1572-9699 (online) VL - Vol. 66 IS - Iss. 1-3 SP - 3 EP - 22 ER - TY - JOUR A1 - Biselli, Manfred A1 - Born, C. A1 - Wandrey, C. T1 - Oxygen transfer from the gasphase to the immobilized cells in membrane aerated fluidized beds / Born, C. ; Biselli, M. ; Wandrey, C. JF - Animal cell technology : basic & applied aspects : proceedings of the Eighth Annual Meeting of the Japanese Association for Animal Cell Technology, Iizuka, Fukuoka, Japan, November 6-10, 1995 / edited by K. Funatsu, Y. Shirai, and T. Matsushita Y1 - 1995 SN - 0-7923-4486-3 N1 - Japanese Association for Animal Cell Technology. ; Meeting ; <8 ; 1995 : ; Iizuka-shi, Japan> SP - 83 EP - 87 PB - Kluwer Acad. Press CY - Boston ER - TY - JOUR A1 - Selmer, Thorsten A1 - Andrei, Paula I. T1 - p-Hydroxyphenylacetate decarboxylase from Clostridium difficile. A novel glycyl radical enzyme catalysing the formation of p-cresol JF - European Journal of Biochemistry. 268 (2001), H. 5 Y1 - 2001 SN - 0014-2956 SP - 1363 EP - 1372 ER - TY - JOUR A1 - Tippkötter, Nils A1 - Roikaew, Wipa A1 - Ulber, Roland A1 - Hoffmann, Alexander A1 - Denzler, Hans-Jörg A1 - Buchholz, Heinrich T1 - Paracoccus denitrificans for the effluent recycling during continuous denitrification of liquid food JF - Biotechnology Progress N2 - Nitrate is an undesirable component of several foods. A typical case of contamination with high nitrate contents is whey concentrate, containing nitrate in concentrations up to 25 l. The microbiological removal of nitrate by Paracoccus denitrificans under formation of harmless nitrogen in combination with a cell retention reactor is described here. Focus lies on the resource-conserving design of a microbal denitrification process. Two methods are compared. The application of polyvinyl alcohol-immobilized cells, which can be applied several times in whey feed, is compared with the implementation of a two step denitrification system. First, the whey concentrate's nitrate is removed by ion exchange and subsequently the eluent regenerated by microorganisms under their retention by crossflow filtration. Nitrite and nitrate concentrations were determined by reflectometric color measurement with a commercially available Reflectoquant® device. Correction factors for these media had to be determined. During the pilot development, bioreactors from 4 to 250 mg·L-1 and crossflow units with membrane areas from 0.02 to 0.80 m2 were examined. Based on the results of the pilot plants, a scaling for the exemplary process of denitrifying 1,000 tons per day is discussed. Y1 - 2010 U6 - http://dx.doi.org/10.1002/btpr.384 SN - 8756-7938 VL - 26 IS - 3 SP - 756 EP - 762 PB - Wiley CY - Hoboken, NJ ER - TY - JOUR A1 - Kotter, Michael A1 - Li, D. X. A1 - Riekert, Lothar T1 - Partial oxidation of o-xylene to phthalic anhydride in a structured fixed bed containing a sequence of catalysts JF - New developments in selective oxidation : proceedings of an international symposium ; Rimini, Italy, Sept. 18 - 22, 1989 / eds.: G. Centi ... - (Studies in surface science and catalysis ; 55) Y1 - 1990 SN - 0-444-88694-X SP - 267 EP - 274 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Kotter, Michael A1 - Xing Li, Dong T1 - Partielle Oxidation von o-Xylol zu Phthalsäreanhydrid an V/Ti-Schalenkatalysatoren : [Synopsis 1898] JF - Chemie - Ingenieur - Technik. 62 (1990), H. 11 Y1 - 1990 SN - 0009-286X N1 - ISSN der E-Ausg.: 1522-2640 ; Das vollständige Manuskript dieser Arbeit umfasst 33 Seiten. Es ist [lt. Verl.] als Fotokopie oder Mikrofiche MS 1898/90 erhältlich. SP - 950 EP - 951 ER - TY - JOUR A1 - Kotter, Michael A1 - Li, D. X. A1 - Riekert, Lothar T1 - Partielle Oxidation von o-Xylol zu Phthalsäureanhydrid in einem strukturierten Festbettreaktor JF - Katalyse : katalytische Abgasreinigung, Zeolithe, homogene Katalyse, selektive Reaktionen, Herstellung und Charakterisierung, Ionenleiter, Elektrokatalyse, SLPC-Katalysatoren, Suspensionsphase ; Vorträge von der DECHEMA-Jahrestagung 1990, 31. Mai und 1. Juni 1990 / hrsg. von H. Kral. - (Deutsche Gesellschaft für Chemisches Apparatewesen, Chemische Technik und Biotechnologie: DECHEMA-Monographien ; 122) Y1 - 1991 SN - 3-527-10216-7 SP - 93 EP - 123 PB - VCH CY - Weinheim ER - TY - JOUR A1 - Schwertner, Eberhard A1 - Berndt, Heinz A1 - Gielen, Hans-Günter A1 - Zahn, Helmut T1 - Peptide 96 : Synthese einiger [2-(p-Biphenylyl)isopropyloxycarbonyl]-Aminosäurederivate JF - Justus Liebigs Annalen der Chemie N2 - Die Darstellung der N-[2-(p-Biphenylyl)isopropyloxycarbonyl]-Derivate (Bpoc-Derivate) des Cysteins unter Verwendung der Thiolschutzgruppen Tetrahydropyranyl (Thp) für 1, Diphenylmethyl (Dpm) für 2, Trityl (Trt) für 3 und S-tert.-Butyl (SBut) für 4 sowie die Synthese von aktivierten Estern der Bpoc-Derivate des Glycins (5), Isoleucins (6) und Prolins (7) werden beschrieben. An einem Beispiel wird die Möglichkeit aufgezeigt, die Bpoc-Gruppe über das Bpoc-Azid nachträglich in den Peptidverband einzuführen. Y1 - 1975 U6 - http://dx.doi.org/10.1002/jlac.197519750318 SN - 1099-0690 VL - 75 IS - 3 SP - 581 EP - 585 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Berndt, Heinz A1 - Zahn, Helmut T1 - Peptide, 99 : Monomere cyclische Cystinpeptidderivate, III ; Synthese der Schafinsulin-A-Kettensequenzen A2–21 und A1–21 als monomere cyclische Dicystinpeptidderivate JF - Justus Liebigs Annalen der Chemie N2 - Die Synthese der Sequenzen A2—21 (13) und A1—21 (15) der Schafinsulin-A-Kette als monomere cyclische Dicystinpeptidderivate wird beschrieben. Die intrachenaren Cystinbrücken A6—7 und A 11 —20 vermitteln die Löslichkeit dieser Derivate in Dimethylformamid und ermöglichen erstmalig die Reindarstellung vollgeschützter Insulin-A-Kettenderivate. Die während der Synthese eingesetzten Schutzgruppen lassen sich mittels Trifluoressigsäure und 2-Mercaptoäthanol quantitativ entfernen. Y1 - 1975 U6 - http://dx.doi.org/10.1002/jlac.197519750908 SN - 1099-0690 VL - 1975 IS - 9 SP - 1601 EP - 1612 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Goetze, Sandra A1 - Baer, Alexandra A1 - Winkelmann, Silke A1 - Nehlsen, Kristina A1 - Seibler, Jost A1 - Maass, Karin A1 - Bode, Jürgen T1 - Performance of genomic bordering elements at predefined genomic loci JF - Molecular and Cellular Biology Y1 - 2005 U6 - http://dx.doi.org/10.1128/MCB.25.6.2260-2272.2005 SN - 1098-5549 VL - 25 IS - 6 SP - 2260 EP - 2272 ER - TY - JOUR A1 - Feuerriegel, Uwe A1 - Stahlberg, R. T1 - Performance of the Thermoselect Demonstration Plant at Fondotoce, Italy: Product Quality and Distribution of Chemical Elements in the Material Flow. Stahlberg, R. ; Feuerriegel, U. JF - Solid waste management: thermal treatment & waste-to-energy technologies : proceedings of an international specialty conference sponsored by the Air & Waste Management Association, [Washington, DC, April 18 - 21, 1995] / comp. by James D. Kilgroe Y1 - 1996 N1 - International Conference on Solid Waste Management, Thermal Treatment and Waste to Energy ; <1995, Washington, DC> PB - Air & Waste Management Assoc. CY - Pittsburgh, Pa. ER - TY - JOUR A1 - Öhlschläger, Peter A1 - Corvinus, Florian M. A1 - Orth, Carina A1 - Moriggl, Richard T1 - Persistent STAT3 activation in colon cancer is associated with enhanced cell proliferation and tumor growth / Corvinus, Florian, Moriggl, Richard ; Tsareva, Svetlana A. ; Wagner, Stefan ; Pfitzner, Edith B. ; Baus, Daniela ; Kaufmann, Roland : Huber, Luka JF - Neoplasia. 7 (2005), H. 6 Y1 - 2005 SN - 1476-5586 SP - 545 EP - 555 ER - TY - JOUR A1 - Luisier, Raphaëlle A1 - Lempiäinen, Harri A1 - Scherbichler, Nina A1 - Braeuning, Albert A1 - Geissler, Miriam A1 - Dubost, Valerie A1 - Müller, Arne A1 - Scheer, Nico A1 - Chibout, Salah-Dine A1 - Hara, Hisanori A1 - Picard, Frank A1 - Theil, Diethilde A1 - Couttet, Philippe A1 - Vitobello, Antonio A1 - Grenet, Olivier A1 - Grasl-Kraupp, Bettina A1 - Ellinger-Ziegelbauer, Heidrung A1 - Thomson, John P. A1 - Meehan, Richard R. A1 - Elcombe, Clifford R. A1 - Henderson, Colin J. A1 - Wolf, C. Roland A1 - Schwarz, Michael A1 - Moulin, Pierre A1 - Terranova, Remi A1 - Moggs, Jonathan G. T1 - Phenobarbital Induces Cell Cycle Transcriptional Responses in Mouse Liver Humanized for Constitutive Androstane and Pregnane X Receptors JF - Toxicological Sciences N2 - The constitutive androstane receptor (CAR) and the pregnane X receptor (PXR) are closely related nuclear receptors involved in drug metabolism and play important roles in the mechanism of phenobarbital (PB)-induced rodent nongenotoxic hepatocarcinogenesis. Here, we have used a humanized CAR/PXR mouse model to examine potential species differences in receptor-dependent mechanisms underlying liver tissue molecular responses to PB. Early and late transcriptomic responses to sustained PB exposure were investigated in liver tissue from double knock-out CAR and PXR (CARᴷᴼ-PXRᴷᴼ), double humanized CAR and PXR (CARʰ-PXRʰ), and wild-type C57BL/6 mice. Wild-type and CARʰ-PXRʰ mouse livers exhibited temporally and quantitatively similar transcriptional responses during 91 days of PB exposure including the sustained induction of the xenobiotic response gene Cyp2b10, the Wnt signaling inhibitor Wisp1, and noncoding RNA biomarkers from the Dlk1-Dio3 locus. Transient induction of DNA replication (Hells, Mcm6, and Esco2) and mitotic genes (Ccnb2, Cdc20, and Cdk1) and the proliferation-related nuclear antigen Mki67 were observed with peak expression occurring between 1 and 7 days PB exposure. All these transcriptional responses were absent in CARᴷᴼ-PXRᴷᴼ mouse livers and largely reversible in wild-type and CARʰ-PXRʰ mouse livers following 91 days of PB exposure and a subsequent 4-week recovery period. Furthermore, PB-mediated upregulation of the noncoding RNA Meg3, which has recently been associated with cellular pluripotency, exhibited a similar dose response and perivenous hepatocyte-specific localization in both wild-type and CARʰ-PXRʰ mice. Thus, mouse livers coexpressing human CAR and PXR support both the xenobiotic metabolizing and the proliferative transcriptional responses following exposure to PB. Y1 - 2014 U6 - http://dx.doi.org/https://doi.org/10.1093/toxsci/kfu038 SN - 1094-2025 VL - 139 IS - 2 SP - 501 EP - 511 PB - Oxford University Press CY - Oxford ER - TY - JOUR A1 - Falkenberg, Fabian A1 - Bott, Michael A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from Bacillaceae JF - Frontiers in Microbiology N2 - The subtilase family (S8), a member of the clan SB of serine proteases are ubiquitous in all kingdoms of life and fulfil different physiological functions. Subtilases are divided in several groups and especially subtilisins are of interest as they are used in various industrial sectors. Therefore, we searched for new subtilisin sequences of the family Bacillaceae using a data mining approach. The obtained 1,400 sequences were phylogenetically classified in the context of the subtilase family. This required an updated comprehensive overview of the different groups within this family. To fill this gap, we conducted a phylogenetic survey of the S8 family with characterised holotypes derived from the MEROPS database. The analysis revealed the presence of eight previously uncharacterised groups and 13 subgroups within the S8 family. The sequences that emerged from the data mining with the set filter parameters were mainly assigned to the subtilisin subgroups of true subtilisins, high-alkaline subtilisins, and phylogenetically intermediate subtilisins and represent an excellent source for new subtilisin candidates. Y1 - 2022 U6 - http://dx.doi.org/10.3389/fmicb.2022.1017978 SN - 1664-302X VL - 2022 IS - 13 PB - Frontiers CY - Lausanne ER - TY - JOUR A1 - Aggarwal, Prerna A1 - Singh, Virendra A1 - Singh, Arjun A1 - Scherer, Ulrich W. A1 - Singh, Tejvir A1 - Singla, Madan L. A1 - Srivastava, Alok T1 - Physico-chemical transformations in swift heavy ion modified poly(ethyleneterephthalate) JF - Radiation Physics and Chemistry Y1 - 2011 SN - 0969-806X N1 - In Press, Accepted Manuscript SP - 1 EP - 28 PB - Pergamon Press CY - Oxford ER -