TY  - JOUR
A1  - Burger, René
A1  - Lindner, Simon
A1  - Rumpf, Jessica
A1  - Do, Xuan Tung
A1  - Diehl, Bernd W.K.
A1  - Rehahn, Matthias
A1  - Monakhova, Yulia
A1  - Schulze, Margit
T1  - Benchtop versus high field NMR: Comparable performance found for the molecular weight determination of lignin
JF  - Journal of Pharmaceutical and Biomedical Analysis
N2  - Lignin is a promising renewable biopolymer being investigated worldwide as an environmentally benign substitute of fossil-based aromatic compounds, e.g. for the use as an excipient with antioxidant and antimicrobial properties in drug delivery or even as active compound. For its successful implementation into process streams, a quick, easy, and reliable method is needed for its molecular weight determination. Here we present a method using 1H spectra of benchtop as well as conventional NMR systems in combination with multivariate data analysis, to determine lignin’s molecular weight (Mw and Mn) and polydispersity index (PDI). A set of 36 organosolv lignin samples (from Miscanthus x giganteus, Paulownia tomentosa and Silphium perfoliatum) was used for the calibration and cross validation, and 17 samples were used as external validation set. Validation errors between 5.6% and 12.9% were achieved for all parameters on all NMR devices (43, 60, 500 and 600 MHz). Surprisingly, no significant difference in the performance of the benchtop and high-field devices was found. This facilitates the application of this method for determining lignin’s molecular weight in an industrial environment because of the low maintenance expenditure, small footprint, ruggedness, and low cost of permanent magnet benchtop NMR systems.
KW  - NMR
KW  - PLS-regression
KW  - Molecular weight determination
KW  - Chemometrics
KW  - Biomass
Y1  - 2022
SN  - 0731-7085
U6  - http://dx.doi.org/10.1016/j.jpba.2022.114649
VL  - 212
IS  - Article number: 114649
PB  - Elsevier
CY  - New York, NY
ER  - 
TY  - JOUR
A1  - Iding, Hans
A1  - Dünnwald, Thomas
A1  - Greiner, Lasse
A1  - Liese, Andreas
A1  - Müller, Michael
A1  - Siegert, Petra
A1  - Grötzinger, Joachim
A1  - Demir, Ayhan S.
A1  - Pohl, Martina
T1  - Benzoylformate Decarboxylase from Pseudomonas putida as Stable Catalyst for the Synthesis of Chiral 2-Hydroxy Ketones
JF  - Chemistry - a European journal
Y1  - 2000
SN  - 1521-3765 (E-Journal); 0947-6539 (Print)
VL  - Vol. 6
IS  - Iss. 8
SP  - 1483
EP  - 1495
ER  - 
TY  - JOUR
A1  - Darmo, J.
A1  - Schäffer, F.
A1  - Förster, Arnold
A1  - Kordos, P.
T1  - Beryllium doped low-temperature-grown MBE GaAs: material for photomixing in the THz frequency range
JF  - ASDAM 2000 : conference proceedings / edited by Jozef Osvald ... [et al.]
Y1  - 2000
SN  - 0780359399
N1  - International Conference on Advanced Semiconductor Devices and Microsystems ; (3rd : ; 2000 : ; Smolenice, Slovakia)
SP  - 147
EP  - 150
PB  - IEEE
CY  - Piscataway, NJ
ER  - 
TY  - JOUR
A1  - Mayer, Claudia
T1  - Between Resistance and Affirmation: Christa Wolf and German Unification
JF  - Canadian review of comparative literature : CRCL = Revue canadienne de littérature comparée / Canadian Comparative Literature Association. 22 (1995), H. 3-4
Y1  - 1995
SN  - 0319-051X
SP  - 813
EP  - 835
ER  - 
TY  - JOUR
A1  - Mayer, Claudia
T1  - Between Trans- and Interdisciplinarity. »Germanistik« and the Dialectic of Inclusion and Exclusion of the Other(s)
JF  - Language and literature today : proceedings of the XIXth triennal congress of the International Federation for Modern Languages and Literatures ; Brasília 22 - 30 August 1993. General ed. Neide de Faria.
Y1  - 1993
SN  - 85-230-0405-x
N1  - Band 2
SP  - 958
EP  - 966
PB  - Univ.
CY  - Brasilia
ER  - 
TY  - JOUR
A1  - Ziemons, Karl
A1  - Bruyndonckx, P.
A1  - Perez, J. M.
A1  - Pietrzyk, U.
A1  - Rato, P.
A1  - Tavernier, S.
T1  - Beyond ClearPET: Next Aims
JF  - 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro Symposium Proceedings ISBI 2008
N2  - The CRYSTAL CLEAR collaboration, in short CCC, is a consortium of 12 academic institutions, mainly from Europe, joining efforts in the area of developing instrumentation for nuclear medicine and medical imaging. In the framework of the CCC a high performance small animal PET system, called ClearPET, was developed by using new technologies in electronics and crystals in a phoswich arrangement combining two types of lutetium- based scintillator materials: LSO:Ce and LuYAP:Ce. Our next aim will be the development of hybrid image systems. Hybrid MR-PET imaging has many unique advantages for brain research. This has sparked a new research line within CCC for the development of novel MR-PET compatible technologies. MRI is not as sensitive as PET but PET has poorer spatial resolution than MRI. Two major advantages of PET are sensitivity and its ability to acquire metabolic information. To assess these innovations, the development of a 9.4T hybrid animal MR-PET scanner is proposed based on an existing 9.4T MR scanner that will be adapted to enable simultaneous acquisition of MR and PET data using cutting- edge technology for both MR and PET.
Y1  - 2008
SN  - 978-1-4244-2003-2
SP  - 1421
EP  - 1424
ER  - 
TY  - JOUR
A1  - Damrau, Karin
T1  - Beyond Solidity - Inventions, Spaces and Concepts for the Elements of Air and Water
JF  - Architectural design : AD. 69 (1999), H. 3-4
Y1  - 1999
SN  - 0003-8504
SP  - 22
EP  - 33
ER  - 
TY  - JOUR
A1  - Blome, Hans-Joachim
A1  - Priester, W.
T1  - Big Bounce in the very early Universe
JF  - Astronomy and Astrophysics. 250 (1991), H. 1
Y1  - 1991
SN  - 0004-6361
SP  - 43
EP  - 49
ER  - 
TY  - CHAP
A1  - Eggert, Mathias
T1  - Big Data Research - How to Structure the Changes of the Past Decade?
T2  - The Art of Structuring
N2  - In the past decade, many IS researchers focused on researching the phenomenon of Big Data. At the same time, the relevance of data protection gets more attention than ever before. In particular, since the enactment of the European General Data Protection Regulation in May 2018 Information Systems research should provide answers for protecting personal data. The article at hand presents a structuring framework for Big Data research outcome and the consideration of data protection. IS Researchers might use the framework in order to structure Big Data literature and to identify research gaps that should be addressed in the future.
Y1  - 2019
SN  - 978-3-030-06234-7
U6  - http://dx.doi.org/10.1007/978-3-030-06234-7_26
SP  - 271
EP  - 281
PB  - Springer
CY  - Cham
ER  - 
TY  - CHAP
A1  - Bechstedt, U.
A1  - Berg, Georg P. A.
A1  - Hacker, U.
A1  - Hardt, Arno
A1  - Hürlimann, W.
A1  - Maier, R.
A1  - Martin, S.
A1  - Meissburger, J.
A1  - Römer, J. G. M.
A1  - Possen, P. von
A1  - Sagefka, T.
T1  - BIG KARL and COSY
T2  - The art and science of magnet design. A Festschrift in honor of Klaus Halbach. Vol. 1
Y1  - 1995
SP  - 19
EP  - 28
PB  - Lawrence Berkeley Lab., Univ. of California
CY  - Berkeley, CA.
ER  - 
TY  - JOUR
A1  - Mansurov, Z.
A1  - Digel, Ilya
A1  - Biisenbaev, M.
A1  - Savistkaya, I.
A1  - Kistaubaeva, A.
A1  - Akimbekov, N.
A1  - Zhubanova, A.
T1  - Bio-composite material on the basis of carbonized rice husk in biomedicine and environmental applications
JF  - Eurasian Chemico-Technological Journal
Y1  - 2012
U6  - http://dx.doi.org/10.18321/ectj105
SN  - 2522-4867
VL  - 14
IS  - 2
SP  - 115
EP  - 131
PB  - Institute of Combustion Problems
CY  - Almaty
ER  - 
TY  - CHAP
A1  - Muffler, Kai
A1  - Poth, Sabastian
A1  - Sieker, Tim
A1  - Tippkötter, Nils
A1  - Ulber, Roland
A1  - Sell, Dieter
ED  - Moo-Young, Murray
T1  - Bio-feedstocks
T2  - Comprehensive biotechnology : principles and practices in industry, agcriculture, medicine and the environment. Volume 2: Engineering fundamentals of biotechnology
Y1  - 2011
SN  - 978-0-444-53352-4
U6  - http://dx.doi.org/10.1016/B978-0-08-088504-9.00088-X
SP  - 93
EP  - 101
PB  - Elsevier
CY  - Amsterdam
ET  - 2. edition
ER  - 
TY  - JOUR
A1  - Uysal, Karya
A1  - Creutz, Till
A1  - Firat, Ipek Seda
A1  - Artmann, Gerhard
A1  - Teusch, Nicole
A1  - Temiz Artmann, Aysegül
T1  - Bio-functionalized ultra-thin, large-area and waterproof silicone membranes for biomechanical cellular loading and compliance experiments
JF  - Polymers
N2  - Biocompatibility, flexibility and durability make polydimethylsiloxane (PDMS) membranes top candidates in biomedical applications. CellDrum technology uses large area, <10 µm thin membranes as mechanical stress sensors of thin cell layers. For this to be successful, the properties (thickness, temperature, dust, wrinkles, etc.) must be precisely controlled. The following parameters of membrane fabrication by means of the Floating-on-Water (FoW) method were investigated: (1) PDMS volume, (2) ambient temperature, (3) membrane deflection and (4) membrane mechanical compliance. Significant differences were found between all PDMS volumes and thicknesses tested (p < 0.01). They also differed from the calculated values. At room temperatures between 22 and 26 °C, significant differences in average thickness values were found, as well as a continuous decrease in thicknesses within a 4 °C temperature elevation. No correlation was found between the membrane thickness groups (between 3–4 µm) in terms of deflection and compliance. We successfully present a fabrication method for thin bio-functionalized membranes in conjunction with a four-step quality management system. The results highlight the importance of tight regulation of production parameters through quality control. The use of membranes described here could also become the basis for material testing on thin, viscous layers such as polymers, dyes and adhesives, which goes far beyond biological applications.
Y1  - 2022
SN  - 2073-4360
VL  - 14
IS  - 11
SP  - 2213
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Thomessen, Karolin
A1  - Thoma, Andreas
A1  - Braun, Carsten
T1  - Bio-inspired altitude changing extension to the 3DVFH* local obstacle avoidance algorithm
JF  - CEAS Aeronautical Journal
N2  - Obstacle avoidance is critical for unmanned aerial vehicles (UAVs) operating autonomously. Obstacle avoidance algorithms either rely on global environment data or local sensor data. Local path planners react to unforeseen objects and plan purely on local sensor information. Similarly, animals need to find feasible paths based on local information about their surroundings. Therefore, their behavior is a valuable source of inspiration for path planning. Bumblebees tend to fly vertically over far-away obstacles and horizontally around close ones, implying two zones for different flight strategies depending on the distance to obstacles. This work enhances the local path planner 3DVFH* with this bio-inspired strategy. The algorithm alters the goal-driven function of the 3DVFH* to climb-preferring if obstacles are far away. Prior experiments with bumblebees led to two definitions of flight zone limits depending on the distance to obstacles, leading to two algorithm variants. Both variants reduce the probability of not reaching the goal of a 3DVFH* implementation in Matlab/Simulink. The best variant, 3DVFH*b-b, reduces this probability from 70.7 to 18.6% in city-like worlds using a strong vertical evasion strategy. Energy consumption is higher, and flight paths are longer compared to the algorithm version with pronounced horizontal evasion tendency. A parameter study analyzes the effect of different weighting factors in the cost function. The best parameter combination shows a failure probability of 6.9% in city-like worlds and reduces energy consumption by 28%. Our findings demonstrate the potential of bio-inspired approaches for improving the performance of local path planning algorithms for UAV.
KW  - UAV
KW  - Obstacle avoidance
KW  - Autonomy
KW  - Local path planning
Y1  - 2023
U6  - http://dx.doi.org/10.1007/s13272-023-00691-w
SN  - 1869-5590 (Online)
SN  - 1869-5582 (Print)
N1  - Corresponding author: Karolin Thomessen
PB  - Springer
CY  - Wien
ER  - 
TY  - JOUR
A1  - Kramer, Friederike
A1  - Halamkova, Lenka
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
A1  - Katz, Evgeny
A1  - Halamek, Jan
T1  - Biocatalytic analysis of biomarkers for forensic identification of ethnicity between Caucasian and African American
JF  - The analyst. August 2013
Y1  - 2013
SN  - 1364-5528 (E-Journal); 0003-2654 (Print)
VL  - Vol. 138
SP  - 6251
EP  - 6257
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Netz, Daili Jacqueline Aguilar
A1  - Pohl, Regula
A1  - Beck-Sickinger, Annette G.
T1  - Biochemical characterisation and genetic analysis of aureocin A53, a new, atypical bacteriocin from Staphylococcus aureus / Netz, Daili Jacqueline Aguilar ; Pohl, Regula ; Beck-Sickinger, Annette G. ; Selmer, Thorsten ; Pierik, Antonio J. ;  Carmo de Frei
JF  - Journal of Molecular Biology. 319 (2002), H. 3
Y1  - 2002
SN  - 0022-2836
SP  - 745
EP  - 756
ER  - 
TY  - JOUR
A1  - Falkenberg, Fabian
A1  - Kohn, Sophie
A1  - Bott, Michael
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
T1  - Biochemical characterisation of a novel broad pH spectrum subtilisin from Fictibacillus arsenicus DSM 15822ᵀ
JF  - FEBS Open Bio
N2  - Subtilisins from microbial sources, especially from the Bacillaceae family, are of particular interest for biotechnological applications and serve the currently growing enzyme market as efficient and novel biocatalysts. Biotechnological applications include use in detergents, cosmetics, leather processing, wastewater treatment and pharmaceuticals. To identify a possible candidate for the enzyme market, here we cloned the gene of the subtilisin SPFA from Fictibacillus arsenicus DSM 15822ᵀ (obtained through a data mining-based search) and expressed it in Bacillus subtilis DB104. After production and purification, the protease showed a molecular mass of 27.57 kDa and a pI of 5.8. SPFA displayed hydrolytic activity at a temperature optimum of 80 °C and a very broad pH optimum between 8.5 and 11.5, with high activity up to pH 12.5. SPFA displayed no NaCl dependence but a high NaCl tolerance, with decreasing activity up to concentrations of 5 m NaCl. The stability enhanced with increasing NaCl concentration. Based on its substrate preference for 10 synthetic peptide 4-nitroanilide substrates with three or four amino acids and its phylogenetic classification, SPFA can be assigned to the subgroup of true subtilisins. Moreover, SPFA exhibited high tolerance to 5% (w/v) SDS and 5% H₂O₂ (v/v). The biochemical properties of SPFA, especially its tolerance of remarkably high pH, SDS and H₂O₂, suggest it has potential for biotechnological applications.
KW  - Bacillaceae
KW  - Biotechnological application
KW  - Broad pH spectrum
KW  - Subtilases
KW  - Subtilisin
Y1  - 2023
U6  - http://dx.doi.org/10.1002/2211-5463.13701
SN  - 2211-5463
N1  - Corresponding author: Petra Siegert
VL  - 13
IS  - 11
SP  - 2035
EP  - 2046
PB  - Wiley
CY  - Hoboken, NJ
ER  - 
TY  - JOUR
A1  - Falkenberg, Fabian
A1  - Rahba, Jade
A1  - Fischer, David
A1  - Bott, Michael
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
T1  - Biochemical characterization of a novel oxidatively stable, halotolerant, and high-alkaline subtilisin from Alkalihalobacillus okhensis Kh10-101T
JF  - FEBS Open Bio
N2  - Halophilic and halotolerant microorganisms represent a promising source of salt-tolerant enzymes suitable for various biotechnological applications where high salt concentrations would otherwise limit enzymatic activity. Considering the current growing enzyme market and the need for more efficient and new biocatalysts, the present study aimed at the characterization of a high-alkaline subtilisin from Alkalihalobacillus okhensis Kh10-101T. The protease gene was cloned and expressed in Bacillus subtilis DB104. The recombinant protease SPAO with 269 amino acids belongs to the subfamily of high-alkaline subtilisins. The biochemical characteristics of purified SPAO were analyzed in comparison with subtilisin Carlsberg, Savinase, and BPN'. SPAO, a monomer with a molecular mass of 27.1 kDa, was active over a wide range of pH 6.0–12.0 and temperature 20–80 °C, optimally at pH 9.0–9.5 and 55 °C. The protease is highly oxidatively stable to hydrogen peroxide and retained 58% of residual activity when incubated at 10 °C with 5% (v/v) H2O2 for 1 h while stimulated at 1% (v/v) H2O2. Furthermore, SPAO was very stable and active at NaCl concentrations up to 5.0 m. This study demonstrates the potential of SPAO for biotechnological applications in the future.
KW  - Alkalihalobacillus okhensis
KW  - detergent protease
KW  - halotolerant protease
KW  - high-alkaline subtilisin
KW  - oxidative stable protease
Y1  - 2022
U6  - http://dx.doi.org/10.1002/2211-5463.13457
SN  - 2211-5463
N1  - Corresponding author: Petra Siegert
VL  - 12
IS  - 10
SP  - 1729
EP  - 1746
PB  - Wiley
CY  - Hoboken, NJ
ER  - 
TY  - JOUR
A1  - Lüth, H.
A1  - Thust, M.
A1  - Steffen, A.
A1  - Kordos, P.
A1  - Schöning, Michael Josef
T1  - Biochemical sensors with structured and porous silicon capacitors
JF  - Materials Science and Engineering B. 69-70 (2000)
Y1  - 2000
SN  - 0921-5107
SP  - 104
EP  - 108
ER  - 
TY  - JOUR
A1  - Akimbekov, N. S.
A1  - Digel, Ilya
A1  - Tastambek, K. T.
A1  - Zhubanova, A. A.
T1  - Biocompatibility of carbonized rice husk with a rat heart cells line H9c2
JF  - Experimental Biology
Y1  - 2013
SN  - 1563-0218
N1  - Original in russischer Sprache
VL  - 59
IS  - 3/1
SP  - 23
EP  - 25
ER  -