TY  - JOUR
A1  - Selmer, Thorsten
A1  - Pinkenburg, Olaf
T1  - Method of cloning at least one nucleic acid molecule of interest using type IIS restriction endonucleases, and corresponding cloning vectors, kits and system using type IIS restriction endonucleases / Selmer, Thorsten ; Pinkenburg, Olaf
Y1  - 2008
N1  - Patent No. WO 2008095927  	A1  	Aug 14, 2008
ER  - 
TY  - PAT
A1  - Stadtmüller, Ralf
A1  - Tippkötter, Nils
A1  - Ulber, Roland
T1  - Method for production of single-stranded macronucleotides
N2  - The invention relates to a method for production of single-stranded macronucleotides by amplifying and ligating an extended monomeric single-stranded target nucleic acid sequence (targetss) into a repetitive cluster of double-stranded target nucleic acid sequences (targetds), and subsequently cloning the construct into a vector (aptagene vector). The aptagene vector is transformed into host cells for replication of the aptagene and isolated in order to optain single-stranded target sequences (targetss). The invention also relates to single-stranded nucleic acids, produced by a method of the invention.
Y1  - 2015
N1  - Patent auch unter EP2774996, EP2774996, US2017145460 und US9944966 veröffentlicht.
ER  - 
TY  - PAT
A1  - O'Connell, Timothy
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
A1  - Schiedel, Marc-Steffen
A1  - Vockenroth, Inga Kerstin
T1  - Method for improving the cleaning action of a detergent or cleaning agent [Internationale Patentanmeldung]
T1  - Verfahren zur Verbesserung der Reinigungsleistung eines Wasch- oder Reinigungsmittels
Y1  - 2010
SP  - 1
EP  - 15
PB  - WIPO
CY  - Genf
ER  - 
TY  - JOUR
A1  - Wilming, Anja
A1  - Begemann, Jens
A1  - Kuhne, Stefan
A1  - Regestein, Lars
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Maurer, Karl-Heinz
A1  - Büchs, Jochen
T1  - Metabolic studies of γ-polyglutamic acid production in Bacillus licheniformis by small-scale continuous cultivations
JF  - Biochemical engineering journal
Y1  - 2013
SN  - 1873-295X (E-Journal); 1369-703X (Print)
VL  - Vol. 73
SP  - 29
EP  - 37
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Huck, Christina
A1  - Schiffels, Johannes
A1  - Herrera, Cony N.
A1  - Schelden, Maximilian
A1  - Selmer, Thorsten
A1  - Poghossian, Arshak
A1  - Baumann, Marcus
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Metabolic responses of Escherichia coli upon glucose pulses captured by a capacitive field-effect sensor
JF  - Physica Status Solidi (A)
N2  - Living cells are complex biological systems transforming metabolites taken up from the surrounding medium. Monitoring the responses of such cells to certain substrate concentrations is a challenging task and offers possibilities to gain insight into the vitality of a community influenced by the growth environment. Cell-based sensors represent a promising platform for monitoring the metabolic activity and thus, the “welfare” of relevant organisms. In the present study, metabolic responses of the model bacterium Escherichia coli in suspension, layered onto a capacitive field-effect structure, were examined to pulses of glucose in the concentration range between 0.05 and 2 mM. It was found that acidification of the surrounding medium takes place immediately after glucose addition and follows Michaelis–Menten kinetic behavior as a function of the glucose concentration. In future, the presented setup can, therefore, be used to study substrate specificities on the enzymatic level and may as well be used to perform investigations of more complex metabolic responses. Conclusions and perspectives highlighting this system are discussed.
Y1  - 2013
U6  - http://dx.doi.org/10.1002/pssa.201200900
SN  - 0031-8965
VL  - 210
IS  - 5
SP  - 926
EP  - 931
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Müller, Ulrike
A1  - Bongaerts, Johannes
A1  - Bovenberg, Roel
A1  - Jossek, Ralf
A1  - Krämer, Marco
A1  - Linnemann, J.
A1  - Müschen, S.
A1  - Ritterbecks, S.
A1  - Sprenger, G.
A1  - Wubbolts, Marcel
T1  - Metabolic engineering to produce fine chemicals in Escherichia coli
JF  - Mededelingen van de Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent
Y1  - 2001
SN  - 0035-533x
VL  - 66 (3a)
SP  - 215
EP  - 217
ER  - 
TY  - JOUR
A1  - Bongaerts, Johannes
A1  - Bovenberg, Roel
A1  - Krämer, Marco
A1  - Müller, Ulrike
A1  - Raeven, Leon
A1  - Wubbolts, Marcel
T1  - Metabolic engineering to produce fine chemicals in Escherichia coli
JF  - Chemie - Ingenieur - Technik (CIT)
Y1  - 2002
SN  - 1522-2640 (E-Journal); 0009-286X (Print)
N1  - Printausg. in der Bibliothek vorhanden: 63 ZS 022
VL  - Vol. 74
IS  - Iss. 5
SP  - 694
ER  - 
TY  - JOUR
A1  - Krämer, Marco
A1  - Bongaerts, Johannes
A1  - Bovenberg, Roel
A1  - Kremer, Susanne
A1  - Müller, Ulrike
A1  - Orf, Sonja
A1  - Wubbolts, Marcel
A1  - Raeven, Leon
T1  - Metabolic engineering for microbial production of shikimic acid
JF  - Metabolic engineering
Y1  - 2003
SN  - 1096-7184 (E-Journal); 1096-7176 (Print)
VL  - Vol. 5
IS  - Iss. 4
SP  - 277
EP  - 283
ER  - 
TY  - JOUR
A1  - Bongaerts, Johannes
A1  - Krämer, Marco
A1  - Müller, Ulrike
A1  - Raeven, Leon
A1  - Wubbolts, Marcel
T1  - Metabolic engineering for microbial production of aromatic amino acids and derived compounds
JF  - Metabolic engineering
Y1  - 2001
SN  - 1096-7184 (E-Journal); 1096-7176 (Print)
VL  - Vol. 3
IS  - Iss. 4
SP  - 289
EP  - 300
ER  - 
TY  - JOUR
A1  - Harish, Ajay B.
A1  - Wriggers, Peter
A1  - Jungk, Juliane
A1  - Hojdis, Nils
A1  - Recker, Carla
T1  - Mesoscale Constitutive Modeling of Non-Crystallizing Filled Elastomers
JF  - Computational Mechanics
N2  - Elastomers are exceptional materials owing to their ability to undergo large deformations before failure. However, due to their very low stiffness, they are not always suitable for industrial applications. Addition of filler particles provides reinforcing effects and thus enhances the material properties that render them more versatile for applications like tyres etc. However, deformation behavior of filled polymers is accompanied by several nonlinear effects like Mullins and Payne effect. To this day, the physical and chemical changes resulting in such nonlinear effect remain an active area of research. In this work, we develop a heterogeneous (or multiphase) constitutive model at the mesoscale explicitly considering filler particle aggregates, elastomeric matrix and their mechanical interaction through an approximate interface layer. The developed constitutive model is used to demonstrate cluster breakage, also, as one of the possible sources for Mullins effect observed in non-crystallizing filled elastomers.
Y1  - 2016
U6  - http://dx.doi.org/10.1007/s00466-015-1251-1
SN  - 1432-0924
VL  - 57
SP  - 653
EP  - 677
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Trapp, Svenja
A1  - Lammers, Tom
A1  - Engudar, Gokce
A1  - Hoehr, Cornelia
A1  - Denkova, Antonia G.
A1  - Paulßen, Elisabeth
A1  - de Kruijff, Robin M.
T1  - Membrane-based microfluidic solvent extraction of Ga-68 from aqueous Zn solutions: towards an automated cyclotron production loop
JF  - EJNMMI Radiopharmacy and Chemistry
KW  - Microfluidic solvent extraction
KW  - Ga-68
KW  - Cyclotron production
KW  - Medical radionuclide production
KW  - Metal contaminants
Y1  - 2023
U6  - http://dx.doi.org/10.1186/s41181-023-00195-2
SN  - 2365-421X
VL  - 2023
IS  - 8, Article number: 9
SP  - 1
EP  - 14
PB  - Springer Nature
ER  - 
TY  - JOUR
A1  - Inagaki, Akiko
A1  - Sleddens-Linkels, Esther
A1  - Wassenaar, Evelyne
A1  - Ooms, Marja
A1  - Cappellen, Wiggert A. van
A1  - Hoeijmakers, Jan H. J.
A1  - Seibler, Jost
A1  - Vogt, Thomas F.
A1  - Shin, Myung K.
A1  - Grootegoed, J. Anton
A1  - Baarends, Willy M.
T1  - Meiotic functions of RAD18
JF  - Journal of Cell Science
Y1  - 2011
U6  - http://dx.doi.org/10.1242/jcs.081968
SN  - 1477-9137
VL  - 124
IS  - 16
SP  - 2837
EP  - 2850
PB  - Company of Biologists Limited
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Seifarth, Volker
A1  - Grosse, Joachim O.
A1  - Grossmann, Matthias
A1  - Janke, Heinz Peter
A1  - Arndt, Patrick
A1  - Koch, Sabine
A1  - Epple, Matthias
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Mechanical induction of bi-directional orientation of primary porcine bladder smooth muscle cells in tubular fibrin-poly(vinylidene fluoride) scaffolds for ureteral and urethral repair using cyclic and focal balloon catheter stimulation
JF  - Journal of Biomaterials Applications
Y1  - 2017
U6  - http://dx.doi.org/10.1177/0885328217723178
SN  - 1530-8022
VL  - 32
IS  - 3
SP  - 321
EP  - 330
PB  - Sage
CY  - London
ER  - 
TY  - JOUR
A1  - Liu, Z.
A1  - Schaap, K. S.
A1  - Ballemans, L.
A1  - de Blois, E.
A1  - Rohde, M.
A1  - Paulßen, Elisabeth
T1  - Measurement of reaction kinetics of [177Lu]Lu-DOTA-TATE using a microfluidic system
JF  - Dalton Transactions
Y1  - 2017
U6  - http://dx.doi.org/10.1039/C7DT01830D
SN  - 1477-9234
VL  - 46
IS  - 42
SP  - 14669
EP  - 14676
ER  - 
TY  - JOUR
A1  - Schmidt, Aaron C.
A1  - Turgut, Hatice
A1  - Le, Dao
A1  - Beloqui, Ana
A1  - Delaittre, Guillaume
T1  - Making the best of it: nitroxide-mediated polymerization of methacrylates via the copolymerization approach with functional styrenics
JF  - Polymer Chemistry
N2  - The SG1-mediated solution polymerization of methyl methacrylate (MMA) and oligo(ethylene glycol) methacrylate (OEGMA, Mₙ = 300 g mol⁻¹) in the presence of a small amount of functional/reactive styrenic comonomer is investigated. Moieties such as pentafluorophenyl ester, triphenylphosphine, azide, pentafluorophenyl, halide, and pyridine are considered. A comonomer fraction as low as 5 mol% typically results in a controlled/living behavior, at least up to 50% conversion. Chain extensions with styrene for both systems were successfully performed. Variation of physical properties such as refractive index (for MMA) and phase transition temperature (for OEGMA) were evaluated by comparing to 100% pure homopolymers. The introduction of an activated ester styrene derivative in the polymerization of OEGMA allows for the synthesis of reactive and hydrophilic polymer brushes with defined thickness. Finally, using the example of pentafluorostyrene as controlling comonomer, it is demonstrated that functional PMMA-b-PS are able to maintain a phase separation ability, as evidenced by the formation of nanostructured thin films.
Y1  - 2020
U6  - http://dx.doi.org/10.1039/C9PY01458F
VL  - 11
IS  - 2
SP  - 593
EP  - 604
PB  - Royal Society of Chemistry (RSC)
CY  - Cambridge
ER  - 
TY  - CHAP
A1  - Tippkötter, Nils
A1  - Möhring, Sophie
A1  - Roth, Jasmine
A1  - Wulfhorst, Helene
T1  - Logistics of lignocellulosic feedstocks: preprocessing as a preferable option
T2  - Biorefineries
N2  - In comparison to crude oil, biorefinery raw materials are challenging in concerns of transport and storage. The plant raw materials are more voluminous, so that shredding and compacting usually are necessary before transport. These mechanical processes can have a negative influence on the subsequent biotechnological processing and shelf life of the raw materials. Various approaches and their effects on renewable raw materials are shown. In addition, aspects of decentralized pretreatment steps are discussed. Another important aspect of pretreatment is the varying composition of the raw materials depending on the growth conditions. This problem can be solved with advanced on-site spectrometric analysis of the material.
KW  - Analytics
KW  - Decentral
KW  - Mechanical
KW  - On-site
KW  - Pre-treatment
Y1  - 2019
SN  - 978-3-319-97117-9
SN  - 978-3-319-97119-3
U6  - http://dx.doi.org/10.1007/10_2017_58
N1  - Advances in biochemical engineering/biotechnology ; Vol. 166
SP  - 43
EP  - 68
PB  - Springer
CY  - Cham
ER  - 
TY  - JOUR
A1  - Prielmeier, Franz
A1  - Hörstermann, D.
A1  - Gyngell, M. L.
A1  - Merboldt, K.-D.
T1  - Localized Proton MRS of Acute and Chronic Gyperglycemia in Rat Brain in vivo / D. Hörstermann, F. Prielmeier , M. L. Gyngell, K.-D. Merboldt, W. Hänicke, J. Frahm
JF  - Book of Abstracts, SMRM, 11th Annual Meeting Berlin
Y1  - 1992
N1  - Society of Magnetic Resonance in Medicine
SP  - 2740
ER  - 
TY  - JOUR
A1  - Poth, Sebastian
A1  - Monzon, Magaly
A1  - Tippkötter, Nils
A1  - Ulber, Roland
T1  - Lignocellulosic biorefinery: Process integration of hydrolysis and fermentation (SSF process)
JF  - Holzforschung
N2  - The aim of the present work is the process integration and the optimization of the enzymatic hydrolysis of wood and the following fermentation of the products to ethanol. The substrate is a fiber fraction obtained by organosolv pre-treatment of beech wood. For the ethanol production, a co-fermentation by two different yeasts (Saccharomyces cerevisiae and Pachysolen tannophilus) was carried out to convert glucose as well as xylose. Two approaches has been followed: 1. A two step process, in which the hydrolysis of the fiber fraction and the fermentation to product are separated from each other. 2. A process, in which the hydrolysis and the fermentation are carried out in one single process step as simultaneous saccharification and fermentation (SSF). Following the first approach, a yield of about 0.15 g ethanol per gram substrate can be reached. Based on the SSF, one process step can be saved, and additionally, the gained yield can be raised up to 0.3 g ethanol per gram substrate.
Y1  - 2011
N1  - 11th EWLP, Hamburg, Germany, August 16–19, 2010
VL  - 65
IS  - 5
SP  - 633
EP  - 637
PB  - De Gruyter
CY  - Berlin
ER  - 
TY  - CHAP
A1  - Poth, Sebastian
A1  - Monzon, Magaly
A1  - Tippkötter, Nils
A1  - Ulber, Roland
T1  - Lignocellulosic biorefinery : process integration of hydrolysis and fermentation
T2  - Proceedings / 11th European Workshop on Lignocellulosics and Pulp : August 16 - 19, 2010, Hamburg, Germany
Y1  - 2010
SP  - 65
EP  - 68
PB  - vTi
CY  - Hamburg
ER  - 
TY  - CHAP
A1  - Duwe, A.
A1  - Tippkötter, Nils
A1  - Ulber, R.
T1  - Lignocellulose-Biorefinery: Ethanol-Focused
T2  - Biorefineries
N2  - The development prospects of the world markets for petroleum and other liquid fuels are diverse and partly contradictory. However, comprehensive changes for the energy supply of the future are essential. Notwithstanding the fact that there are still very large deposits of energy resources from a geological point of view, the finite nature of conventional oil reserves is indisputable. To reduce our dependence on oil, the EU, the USA, and other major economic zones rely on energy diversification. For this purpose, alternative materials and technologies are being sought, and is most obvious in the transport sector. The objective is to progressively replace fossil fuels with renewable and more sustainable fuels. In this respect, biofuels have a pre-eminent position in terms of their capability of blending with fossil fuels and being usable in existing cars without substantial modification. Ethanol can be considered as the primary renewable liquid fuel. In this chapter enzymes, micro-organisms, and processes for ethanol production based on renewable resources are described.
KW  - Bioethanol
KW  - Biorefinery
KW  - Lignocellulose feedstook
Y1  - 2018
U6  - http://dx.doi.org/10.1007/10_2016_72
N1  - Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE,volume 166)
SP  - 177
EP  - 215
PB  - Springer
CY  - Cham
ER  -