TY  - JOUR
A1  - Dantism, Shahriar
A1  - Röhlen, Desiree
A1  - Wagner, Torsten
A1  - Wagner, P.
A1  - Schöning, Michael Josef
T1  - A LAPS-based differential sensor for parallelized metabolism monitoring of various bacteria
JF  - Sensors
N2  - Monitoring the cellular metabolism of bacteria in (bio)fermentation processes is crucial to control and steer them, and to prevent undesired disturbances linked to metabolically inactive microorganisms. In this context, cell-based biosensors can play an important role to improve the quality and increase the yield of such processes. This work describes the simultaneous analysis of the metabolic behavior of three different types of bacteria by means of a differential light-addressable potentiometric sensor (LAPS) set-up. The study includes Lactobacillus brevis, Corynebacterium glutamicum, and Escherichia coli, which are often applied in fermentation processes in bioreactors. Differential measurements were carried out to compensate undesirable influences such as sensor signal drift, and pH value variation during the measurements. Furthermore, calibration curves of the cellular metabolism were established as a function of the glucose concentration or cell number variation with all three model microorganisms. In this context, simultaneous (bio)sensing with the multi-organism LAPS-based set-up can open new possibilities for a cost-effective, rapid detection of the extracellular acidification of bacteria on a single sensor chip. It can be applied to evaluate the metabolic response of bacteria populations in a (bio)fermentation process, for instance, in the biogas fermentation process.
Y1  - 2019
U6  - http://dx.doi.org/10.3390/s19214692
SN  - 1424-8220
VL  - 19
IS  - 21
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Bhattarai, Aroj
A1  - Staat, Manfred
T1  - A computational study of organ relocation after laparoscopic pectopexy to repair posthysterectomy vaginal vault prolapse
JF  - Computer Methods in Biomechanics and Biomedical Engineering: Imaging & Visualization
Y1  - 2019
U6  - http://dx.doi.org/10.1080/21681163.2019.1670095
SN  - 2168-1171
PB  - Taylor & Francis
CY  - London
ER  - 
TY  - JOUR
A1  - Achtsnicht, Stefan
A1  - Tödter, Julia
A1  - Niehues, Julia
A1  - Telöken, Matthias
A1  - Offenhäusser, Andreas
A1  - Krause, Hans-Joachim
A1  - Schröper, Florian
T1  - 3D printed modular immunofiltration columns for frequency mixing-based multiplex magnetic immunodetection
JF  - Sensors
N2  - For performing point-of-care molecular diagnostics, magnetic immunoassays constitute a promising alternative to established enzyme-linked immunosorbent assays (ELISA) because they are fast, robust and sensitive. Simultaneous detection of multiple biomolecular targets from one body fluid sample is desired. The aim of this work is to show that multiplex magnetic immunodetection based on magnetic frequency mixing by means of modular immunofiltration columns prepared for different targets is feasible. By calculations of the magnetic response signal, the required spacing between the modules was determined. Immunofiltration columns were manufactured by 3D printing and antibody immobilization was performed in a batch approach. It was shown experimentally that two different target molecules in a sample solution could be individually detected in a single assaying step with magnetic measurements of the corresponding immobilization filters. The arrangement order of the filters and of a negative control did not influence the results. Thus, a simple and reliable approach to multi-target magnetic immunodetection was demonstrated.
Y1  - 2019
U6  - http://dx.doi.org/10.3390/s19010148
SN  - 1424-8220
VL  - 19
IS  - 1
PB  - MDPI
CY  - Basel
ER  -