TY  - JOUR
A1  - Takenaga, Shoko
A1  - Biselli, Manfred
A1  - Schnitzler, Thomas
A1  - Öhlschläger, Peter
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
T1  - Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis–Menten-like kinetics for cell culturing
JF  - Physica status solidi A : Applications and materials science
N2  - The metabolic activity of Chinese hamster ovary (CHO) cells was observed using a light-addressable potentiometric sensor (LAPS). The dependency toward different glucose concentrations (17–200 mM) follows a Michaelis–Menten kinetics trajectory with Kₘ = 32.8 mM, and the obtained Kₘ value in this experiment was compared with that found in literature. In addition, the pH shift induced by glucose metabolism of tumor cells transfected with the HPV-16 genome (C3 cells) was successfully observed. These results indicate the possibility to determine the tumor cells metabolism with a LAPS-based measurement device.
Y1  - 2014
U6  - http://dx.doi.org/10.1002/pssa.201330464
SN  - 1521-396X (E); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print)
VL  - 211
IS  - 6
SP  - 1410
EP  - 1415
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Sawada, Kazuaki
A1  - Nakazawa, Hirokazu
A1  - Takenaga, Shoko
A1  - Hizawa, Takeshi
A1  - Futagawa, Masato
A1  - Dasai, Fumihiro
A1  - Sakurai, Takashi
A1  - Okumura, Koichi
A1  - Hattori, Toshiaki
A1  - Ishida, Makoto
T1  - Multimodal bioimage sensor
JF  - IEICE transactions on fundamentals of electronics, communidations and computer sciences
N2  - To visualize the biochemical distribution two-dimensionally, we invented a solid-state-type ion image sensor that indicates the chemical activity of solutions and cells. The device, which consists of a CCD array covered with a functionalized membrane to detect charge accumulation, is highly sensitive to changes in the concentration and two-dimensional distribution of ions and biomaterials.
Y1  - 2014
U6  - http://dx.doi.org/10.1587/transfun.E97.A.726
SN  - 0916-8508 (Print) ; 1745-1337 (Online)
VL  - E97-A (2014)
IS  - 3
SP  - 726
EP  - 733
PB  - IEICE
CY  - Tokyo
ER  -