TY  - JOUR
A1  - Werner, Frederik
A1  - Wagner, Torsten
A1  - Miyamoto, Ko-ichiro
A1  - Yoshinobu, Tatsuo
A1  - Schöning, Michael Josef
T1  - High speed and high resolution chemical imaging based on a new type of OLED-LAPS set-up
JF  - Procedia Engineering. 25 (2011)
Y1  - 2011
SN  - 1877-7058
N1  - EurosensorsXXV ; Proc. Eurosensors XXV, September 4-7, 2011, Athens, Greece
SP  - 346
EP  - 349
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Moritz, W.
A1  - Yoshinobu, T.
A1  - Finger, F.
A1  - Krause, S.
A1  - Martin-Fernandez, M.
A1  - Schöning, Michael Josef
T1  - High resolution LAPS using amorphous silicon as the semiconductor material
JF  - Sensors and Actuators B. 103 (2004), H. 1-2
Y1  - 2004
SN  - 0925-4005
SP  - 436
EP  - 444
ER  - 
TY  - JOUR
A1  - Bressel, A.
A1  - Schultze, J.W.
A1  - Khan, W.
A1  - Wolfaardt, G. M.
A1  - Rohns, H.-P.
A1  - Irmscher, R.
A1  - Schöning, Michael Josef
T1  - High resolution gravimetric, optical and electrochemical investigations of microbial biofilm formation in aqueous systems
JF  - Electrochimica Acta. 48 (2003), H. 20-22
Y1  - 2003
SN  - 0013-4686
SP  - 3363
EP  - 3372
ER  - 
TY  - JOUR
A1  - Heine, A.
A1  - Herrmann, G.
A1  - Selmer, Thorsten
A1  - Terwesten, F.
A1  - Buckel, W.
A1  - Reuter, K.
T1  - High resolution crystal structure of clostridium propionicum β-Alanyl-CoA:Ammonia Lyase, a new member of the "Hot Dog Fold" protein superfamily
JF  - Proteins
N2  - Clostridium propionicum is the only organism known to ferment β-alanine, a constituent of coenzyme A (CoA) and the phosphopantetheinyl prosthetic group of holo-acyl carrier protein. The first step in the fermentation is a CoA-transfer to β-alanine. Subsequently, the resulting β-alanyl-CoA is deaminated by the enzyme β-alanyl-CoA:ammonia lyase (Acl) to reversibly form ammonia and acrylyl-CoA. We have determined the crystal structure of Acl in its apo-form at a resolution of 0.97 Å as well as in complex with CoA at a resolution of 1.59 Å. The structures reveal that the enyzme belongs to a superfamily of proteins exhibiting a so called “hot dog fold” which is characterized by a five-stranded antiparallel β-sheet with a long α-helix packed against it. The functional unit of all “hot dog fold” proteins is a homodimer containing two equivalent substrate binding sites which are established by the dimer interface. In the case of Acl, three functional dimers combine to a homohexamer strongly resembling the homohexamer formed by YciA-like acyl-CoA thioesterases. Here, we propose an enzymatic mechanism based on the crystal structure of the Acl·CoA complex and molecular docking. Proteins 2014; 82:2041–2053. © 2014 Wiley Periodicals, Inc.
Y1  - 2014
U6  - http://dx.doi.org/10.1002/prot.24557
SN  - 1097-0134 (E-Journal); 0887-3585 (Print)
VL  - 82
IS  - 9
SP  - 2041
EP  - 2053
PB  - Wiley-Liss
CY  - New York
ER  - 
TY  - JOUR
A1  - Turek, Monika
A1  - Schöning, Michael Josef
A1  - Kloock, Joachim P.
A1  - Schubert, Jürgen
A1  - Zander, Willi
A1  - Krüger, Peter
A1  - Keusgen, Michael
T1  - Herstellung und Charakterisierung eines hybriden Sensorarrays auf Halbleiterbasis für die Umweltanalytik
JF  - Sensoren und Messsysteme 2008 : 14. Fachtagung Ludwigsburg, 11. und 12. März 2008 / VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik
Y1  - 2008
SN  - 978-3-18-092011-5
N1  - VDI-Berichte ; 2011 ; Sensoren und Messsysteme 2008, 14. GMA/ITG-Fachtagung, VDI/VDE- Gesellschaft Mess- und Automatisierungstechnik, + CD-ROM, Ludwigsburg, DE, 11.-12. Mar, 2008
SP  - 803
EP  - 810
PB  - VDI-Verl.
CY  - Düsseldorf
ER  - 
TY  - CHAP
A1  - Kloock, Joachim P.
A1  - Schöning, Michael Josef
T1  - Heavy metal detection with semiconductor devices based on PLD-prepared chalcogenide glass thin films
T2  - Armenian Journal of Physics
Y1  - 2007
SN  - 1829-1171
SP  - 95
EP  - 98
ER  - 
TY  - JOUR
A1  - Grinsven, Bart van
A1  - Bon, Natalie vanden
A1  - Strauven, Hannelore
A1  - Grieten, Lars
A1  - Murib, Mohammed
A1  - Jiménez Monroy, Kathia L.
A1  - Janssens, Stoffel D.
A1  - Haenen, Ken
A1  - Schöning, Michael Josef
A1  - Vermeeren, Veronique
A1  - Ameloot, Marcel
A1  - Michiels, Luc
A1  - Thoelen, Ronald
A1  - Ceuninck, Ward de
A1  - Wagner, Patrick
T1  - Heat-Transfer Resistance at Solid-Liquid Interfaces: A Tool for The Detection of Single Nucleotide Polymorphisms in DNA.
JF  - ACS Nano
N2  - In this article, we report on the heat-transfer resistance at interfaces as a novel, denaturation-based method to detect single-nucleotide polymorphisms in DNA. We observed that a molecular brush of double-stranded DNA grafted onto synthetic diamond surfaces does not notably affect the heat-transfer resistance at the solid-to-liquid interface. In contrast to this, molecular brushes of single-stranded DNA cause, surprisingly, a substantially higher heat-transfer resistance and behave like a thermally insulating layer. This effect can be utilized to identify ds-DNA melting temperatures via the switching from low- to high heat-transfer resistance. The melting temperatures identified with this method for different DNA duplexes (29 base pairs without and with built-in mutations) correlate nicely with data calculated by modeling. The method is fast, label-free (without the need for fluorescent or radioactive markers), allows for repetitive measurements, and can also be extended toward array formats. Reference measurements by confocal fluorescence microscopy and impedance spectroscopy confirm that the switching of heat-transfer resistance upon denaturation is indeed related to the thermal on-chip denaturation of DNA.
Y1  - 2012
U6  - http://dx.doi.org/10.1021/nn300147e
SN  - 1936-086X
VL  - 6
IS  - 3
SP  - 2712
EP  - 2721
PB  - ACS Publications
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Murib, M. S.
A1  - Yeap, W. S.
A1  - Eurlings, Y.
A1  - Grinsven, B. van
A1  - Boyen, H.-G.
A1  - Conings, B.
A1  - Michiels, L.
A1  - Ameloot, M.
A1  - Carleer, R.
A1  - Warmer, J.
A1  - Kaul, P.
A1  - Haenen, K.
A1  - Schöning, Michael Josef
A1  - Ceuninck, W. de
A1  - Wagner, P.
T1  - Heat-transfer based characterization of DNA on synthetic sapphire chips
JF  - Sensors and Actuators B: Chemical
N2  - In this study, we show that synthetic sapphire (Al₂O₃), an established implant material, can also serve as a platform material for biosensors comparable to nanocrystalline diamond. Sapphire chips, beads, and powder were first modified with (3-aminopropyl) triethoxysilane (APTES), followed by succinic anhydride (SA), and finally single-stranded probe DNA was EDC coupled to the functionalized layer. The presence of the APTES-SA layer on sapphire powders was confirmed by thermogravimetric analyis and Fourier-transform infrared spectroscopy. Using planar sapphire chips as substrates and X-ray photoelectron spectroscopy (XPS) as surface-sensitive tool, the sequence of individual layers was analyzed with respect to their chemical state, enabling the quantification of areal densities of the involved molecular units. Fluorescence microscopy was used to demonstrate the hybridization of fluorescently tagged target DNA to the probe DNA, including denaturation- and re-hybridization experiments. Due to its high thermal conductivity, synthetic sapphire is especially suitable as a chip material for the heat-transfer method, which was employed to distinguish complementary- and non-complementary DNA duplexes containing single-nucleotide polymorphisms. These results indicate that it is possible to detect mutations electronically with a chemically resilient and electrically insulating chip material.
Y1  - 2016
U6  - http://dx.doi.org/10.1016/j.snb.2016.02.027
SN  - 0925-4005
VL  - 230
IS  - 230
SP  - 260
EP  - 271
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Wagner, Torsten
A1  - Molina, Roberto
A1  - Yoshinobu, Tatsuo
A1  - Kloock, Joachim P.
A1  - Biselli, Manfred
A1  - Canzoneri, Michele
A1  - Schnitzler, Thomas
A1  - Schöning, Michael Josef
T1  - Handheld multi-channel LAPS device as a transducer platform for possible biological and chemical multi-sensor applications
JF  - Electrochimica Acta. 53 (2007), H. 2
Y1  - 2007
SN  - 0013-4686
SP  - 305
EP  - 311
ER  - 
TY  - JOUR
A1  - Wagner, Torsten
A1  - Maris, Rob J.
A1  - Ackermann, Hans-Josef
A1  - Otto, Ralph
A1  - Beging, Stefan
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
T1  - Handheld measurement device for field-effect sensor structures: Practical evaluation and limitations
JF  - Sensors and Actuators B: Chemical  . 127 (2007), H. 1
Y1  - 2007
SN  - 0925-4005
SP  - 217
EP  - 223
ER  - 
TY  - CHAP
A1  - Kloock, Joachim P.
A1  - Moreno, Lia
A1  - Huachupoma, S.
A1  - Xu, J.
A1  - Wagner, Torsten
A1  - Bratov, A.
A1  - Doll, T.
A1  - Vlasov, Y.
A1  - Schöning, Michael Josef
ED  - Gerlach, Gerald
T1  - Halbleiterbasierte Schwermetallsensorik auf der Basis von Chalkogenidgläsern für zukünftige „Lab on Chip“-Anwendungen
T2  - 7. Dresdner Sensor-Symposium - Neue Herausforderungen und Anwendungen in der Sensortechnik
Y1  - 2005
SN  - 3-938863-29-3
SP  - 221
EP  - 224
PB  - TUDpress, Verl. der Wissenschaften
CY  - Dresden
ER  - 
TY  - JOUR
A1  - Näther, Niko
A1  - Berger, Jörg
A1  - Emmerich, Rüdiger
A1  - Friedrich, P.
A1  - Henkel, H.
A1  - Schöning, Michael Josef
T1  - H2O2-Konzentrationsbestimmung für die Prozessgasanalytik mittels eines kalorimetrischen Sensoraufbaus
JF  - Sensoren und Messsysteme 2008 : 14. Fachtagung Ludwigsburg, 11. und 12. März 2008 / VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik
Y1  - 2008
SN  - 978-3-18-092011-5
N1  - VDI-Berichte ; 2011 ; Sensoren und Messsysteme 2008, 14. GMA/ITG-Fachtagung, VDI/VDE- Gesellschaft Mess- und Automatisierungstechnik, + CD-ROM, Ludwigsburg, DE, 11.-12. Mar, 2008
SP  - 783
EP  - 792
PB  - VDI-Verl.
CY  - Düsseldorf
ER  - 
TY  - JOUR
A1  - Karschuck, Tobias
A1  - Kaulen, Corinna
A1  - Poghossian, Arshak
A1  - Wagner, Patrick H.
A1  - Schöning, Michael Josef
T1  - Gold nanoparticle-modified capacitive field-effect sensors: Studying the surface density of nanoparticles and coupling of charged polyelectrolyte macromolecules
JF  - Electrochemical Science Advances
N2  - The coupling of ligand-stabilized gold nanoparticles with field-effect devices offers new possibilities for label-free biosensing. In this work, we study the immobilization of aminooctanethiol-stabilized gold nanoparticles (AuAOTs) on the silicon dioxide surface of a capacitive field-effect sensor. The terminal amino group of the AuAOT is well suited for the functionalization with biomolecules. The attachment of the positively-charged AuAOTs on a capacitive field-effect sensor was detected by direct electrical readout using capacitance-voltage and constant capacitance measurements. With a higher particle density on the sensor surface, the measured signal change was correspondingly more pronounced. The results demonstrate the ability of capacitive field-effect sensors for the non-destructive quantitative validation of nanoparticle immobilization. In addition, the electrostatic binding of the polyanion polystyrene sulfonate to the AuAOT-modified sensor surface was studied as a model system for the label-free detection of charged macromolecules. Most likely, this approach can be transferred to the label-free detection of other charged molecules such as enzymes or antibodies.
KW  - polystyrene sulfonate
KW  - gold nanoparticles
KW  - field-effect sensor
KW  - detection of charged macromolecules
KW  - capacitive EIS sensor
Y1  - 2021
U6  - http://dx.doi.org/10.1002/elsa.202100179
SN  - 0938-5193
VL  - 2
IS  - 5
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Deppe, Veronika Maria
A1  - Klatte, Stephanie
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - O'Connell, Timothy
A1  - Meinhardt, Friedhelm
T1  - Genetic control of Amadori product degradation in Bacillus subtilis via regulation of frlBONMD expression by FrlR
JF  - Applied and environmental microbiology
Y1  - 2011
SN  - 1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)
VL  - Vol. 77
IS  - No. 9
SP  - 2839
EP  - 2846
PB  - American Society of Mechanical Engineers (ASME)
CY  - New York
ER  - 
TY  - JOUR
A1  - Borgmeier, Claudia
A1  - Bongaerts, Johannes
A1  - Meinhardt, Friedhelm
T1  - Genetic analysis of the Bacillus licheniformis degSU operon and the impact of regulatory mutations on protease production
JF  - Journal of biotechnology
N2  - Disruption experiments targeted at the Bacillus licheniformis degSU operon and GFP-reporter analysis provided evidence for promoter activity immediately upstream of degU. pMutin mediated concomitant introduction of the degU32 allele – known to cause hypersecretion in Bacillus subtilis – resulted in a marked increase in protease activity. Application of 5-fluorouracil based counterselection through establishment of a phosphoribosyltransferase deficient Δupp strain eventually facilitated the marker-free introduction of degU32 leading to further protease enhancement achieving levels as for hypersecreting wild strains in which degU was overexpressed. Surprisingly, deletion of rapG – known to interfere with DegU DNA-binding in B. subtilis – did not enhance protease production neither in the wild type nor in the degU32 strain. The combination of degU32 and Δupp counterselection in the type strain is not only equally effective as in hypersecreting wild strains with respect to protease production but furthermore facilitates genetic strain improvement aiming at biological containment and effectiveness of biotechnological processes.
KW  - Marker-free mutagenesis
KW  - Extracellular enzymes
KW  - Uracil-phosphoribosyltransferase
KW  - Hypersecretion
Y1  - 2012
U6  - http://dx.doi.org/10.1016/j.jbiotec.2012.02.011
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - 159
IS  - 1-2
SP  - 12
EP  - 20
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Mikulics, M.
A1  - Camara, I.
A1  - Hardt, A. van der
A1  - Fox, A.
A1  - Förster, Arnold
A1  - Gusten, R.
A1  - Lüth, H.
A1  - Kordos, P.
T1  - Generation of THz radiation by photomixing in low-temperature-grown MBE GaAs
JF  - Fifth International Conference on Advanced Semiconductor Devices and Microsystems : conference proceedings ; Smolenice Castle, Slovakia, October 17 - 21, 2004 / [organizers: Institute of Electrical Engineering, Slovak Academy of Sciences, Bratislava and Microelectronics Department, Faculty of Electrical Engineering and Information Technology, Slovak University of Technology, Bratislava]. Ed. by J. Osvald
Y1  - 2004
SN  - 0-7803-8335-7
N1  - International Conference on Advanced Semiconductor Devices and Microsystems <5, 2004, Smolenice> ; ASDAM <5, 2004, Smolenice> ; IEEE catalog numer: 04EX867
SP  - 231
EP  - 234
PB  - IEEE Operations Center
CY  - Piscataway, NJ
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Bäcker, Matthias
A1  - Mayer, Dirk
A1  - Schöning, Michael Josef
T1  - Gating capacitive field-effect sensors by the charge of nanoparticle/molecule hybrids
JF  - Nanoscale
Y1  - 2015
U6  - http://dx.doi.org/10.1039/C4NR05987E
SN  - 2040-3372 (E-Journal); 2040-3364 (Print)
SP  - 1023
EP  - 1031
PB  - Royal Society of Chemistry (RSC)
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Kirchner, Patrick
A1  - Ng, Yue Ann
A1  - Spelthahn, Heiko
A1  - Schneider, Andreas
A1  - Henkel, Hartmut
A1  - Friedrich, Peter
A1  - Kolstad, Jens
A1  - Berger, Jörg
A1  - Keusgen, Michael
T1  - Gas sensor investigation based on a catalytically activated thin-film thermopile for H2O2 detection
JF  - Physica Status Solidi (A). 207 (2010), H. 4
Y1  - 2010
SN  - 1862-6300
N1  - Special Issue: Engineering of Functional Interfaces EnFI 2009
SP  - 787
EP  - 792
ER  - 
TY  - JOUR
A1  - Kloock, Joachim P.
A1  - Poghossian, Arshak
A1  - Schumacher, K.
A1  - Rosenkranz, C.
A1  - Schultze, J. W.
A1  - Müller-Veggian, Mattea
A1  - Schöning, Michael Josef
T1  - Funktionsprüfung und Charakterisierung von ionensensitiven Feldeffekttransistoren (ISFETs) auf Waferebene mittels Mikrotropfenzelle für den zukünftigen Einsatz in der Sensorproduktion
JF  - Sensoren und Messsysteme 2006 : Vorträge der 13. ITG/GMA-Fachtagung vom 13. bis 14. März 2006 in Freiburg/Breisgau / Veranst.: Informationstechnische Gesellschaft im VDE (ITG) ; VDE/VDI-Gesellschaft Mess- und Automatisierungstechnik (GMA). Wiss. Tagungsleitung: L. M. Reindl
Y1  - 2006
SN  - 3-8007-2939-3
N1  - Fachtagung Sensoren und Messsysteme <13, 2006, Freiburg, Breisgau>
SP  - 257
EP  - 260
PB  - VDE-Verl.
CY  - Berlin
ER  - 
TY  - CHAP
A1  - Poghossian, Arshak
A1  - Schumacher, Kerstin
A1  - Kloock, Joachim P.
A1  - Rosenkranz, Christian
A1  - Schultze, Joachim W.
A1  - Müller-Veggian, Mattea
A1  - Schöning, Michael Josef
T1  - Functional testing and characterisation of ISFETs on wafer level by means of a micro-droplet cell
N2  - A wafer-level functionality testing and characterisation system for ISFETs (ionsensitive field-effect transistor) is realised by means of integration of a specifically designed capillary electrochemical micro-droplet cell into a commercial wafer prober-station. The developed system allows the identification and selection of “good” ISFETs at the earliest stage and to avoid expensive bonding, encapsulation and packaging processes for nonfunctioning ISFETs and thus, to decrease costs, which are wasted for bad dies. The developed system is also feasible for wafer-level characterisation of ISFETs in terms of sensitivity, hysteresis and response time. Additionally, the system might be also utilised for wafer-level testing of further electrochemical sensors.
KW  - Biosensor
KW  - Biosensorik
KW  - ISFET
KW  - Wafer
KW  - ISFET
KW  - wafer-level testing
KW  - capillary micro-droplet cell
Y1  - 2006
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:a96-opus-1259
ER  -