TY  - JOUR
A1  - Barbazán, Paula
A1  - Hagenbach, Adelheid
A1  - Paulßen, Elisabeth
A1  - Abram, Ulrich
A1  - Carballo, Rosa
A1  - Rodriguez-Hermida, Sabina
A1  - Vázquez-López, Ezequiel M.
T1  - Tricarbonyl Rhenium(I) and Technetium(I) Complexes with Hydrazones Derived from 4,5-Diazafluoren-9-one and 1,10-Phenanthroline-5,6-dione
JF  - European Journal of Inorganic Chemistry
N2  - Tricarbonylrhenium(I) and -technetium(I) halide (halide = Cl and Br) complexes of ligands derived from 4,5-diazafluoren-9-one (df) and 1,10-phenanthroline-5,6-dione (phen) derivatives of benzoic and 2-hydroxybenzoic acid hydrazides have been prepared. The complexes have been characterized by elemental analysis, MS, IR, 1H NMR and absorption and emission UV/Vis spectroscopic methods. The metal centres (ReI and TcI) are coordinated through the nitrogen imine atoms and establish five-membered chelate rings, whereas the hydrazone groups stand uncoordinated. The 1H NMR spectra suggest the same behaviour in solution on the basis of only marginal variations in the chemical shifts of the hydrazine protons.
Y1  - 2010
U6  - http://dx.doi.org/10.1002/ejic.201000522
SN  - 1099-0682
IS  - 29
SP  - 4622
EP  - 4630
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Paulßen, Elisabeth
A1  - Schweighöfer, Philip V.
A1  - Abram, Ulrich
T1  - Reactions of [ReOX3(PPh3)2] Complexes (X = Cl, Br) with Phenylacetylene and the Structures of the Products
JF  - Zeitschrift für anorganische und allgemeine Chemie : ZAAC = Journal of inorganic and general chemistry
N2  - Oxorhenium(V) complexes [ReOX3(PPh3)2] (X = Cl, Br) react with phenylacetylene under formation of complexes with ylide-type ligands. Compounds of the compositions [ReOCl3(PPh3){C(Ph)C(H)(PPh3)}] (1), [ReOBr3(OPPh3){C(Ph)C(H)(PPh3)}] (2), and [ReOBr3(OPPh3){C(H)C(Ph)(PPh3)}] (3) were isolated and characterized by X-ray diffraction. They contain a ligand, which was formed by a nucleophilic attack of released PPh3 at coordinated phenylacetylene. The structures of the products show that there is no preferable position for this attack. Cleavage of the Re–C bond in 3 and dimerization of the organic ligand resulted in the formation of the [{(PPh3)(H)CC(Ph)}2]2+ cation, which crystallized as its [(ReOBr4)(OReO3)]2– salt.
Y1  - 2010
U6  - http://dx.doi.org/10.1002/zaac.200900478
SN  - 1521-3749
VL  - 636
IS  - 5
SP  - 779
EP  - 783
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Paulßen, Elisabeth
A1  - Alberto, Roger
A1  - Abram, Ulrich
T1  - Synthesis, Characterization, and Structures of R3EOTcO3 Complexes (E = C, Si, Ge, Sn, Pb) and Related Compounds
JF  - Inorganic Chemistry
N2  - AgTcO4 reacts with R3ECl compounds (E = C, Si, Ge, Sn, Pb; R = Me, iPr, tBu, Ph), tBu2SnCl2, or PhMgCl under formation of novel trioxotechnetium(VII) derivatives. The carbon and silicon derivatives readily undergo decomposition, which was proven by 99Tc NMR spectroscopy and the isolation of decomposition products such as [TcOCl3(THF)(OH2)]. Compounds [Ph3GeOTcO3], [(THF)Ph3SnOTcO3], [(O3TcO)SntBu2(OH)]2, and [(THF)4Mg(OTcO3)2] are more stable and were isolated in crystalline form and characterized by X-ray diffraction.
Y1  - 2010
U6  - http://dx.doi.org/10.1021/ic1001094
SN  - 1520-510X
VL  - 49
IS  - 7
SP  - 3525
EP  - 3530
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Roikaew, Wipa
A1  - Ulber, Roland
A1  - Hoffmann, Alexander
A1  - Denzler, Hans-Jörg
A1  - Buchholz, Heinrich
T1  - Paracoccus denitrificans for the effluent recycling during continuous denitrification of liquid food
JF  - Biotechnology Progress
N2  - Nitrate is an undesirable component of several foods. A typical case of contamination with high nitrate contents is whey concentrate, containing nitrate in concentrations up to 25 l. The microbiological removal of nitrate by Paracoccus denitrificans under formation of harmless nitrogen in combination with a cell retention reactor is described here. Focus lies on the resource-conserving design of a microbal denitrification process. Two methods are compared. The application of polyvinyl alcohol-immobilized cells, which can be applied several times in whey feed, is compared with the implementation of a two step denitrification system. First, the whey concentrate's nitrate is removed by ion exchange and subsequently the eluent regenerated by microorganisms under their retention by crossflow filtration. Nitrite and nitrate concentrations were determined by reflectometric color measurement with a commercially available Reflectoquant® device. Correction factors for these media had to be determined. During the pilot development, bioreactors from 4 to 250 mg·L-1 and crossflow units with membrane areas from 0.02 to 0.80 m2 were examined. Based on the results of the pilot plants, a scaling for the exemplary process of denitrifying 1,000 tons per day is discussed.
Y1  - 2010
U6  - http://dx.doi.org/10.1002/btpr.384
SN  - 8756-7938
VL  - 26
IS  - 3
SP  - 756
EP  - 762
PB  - Wiley
CY  - Hoboken, NJ
ER  - 
TY  - JOUR
A1  - Degering, Christian
A1  - Eggert, Thorsten
A1  - Puls, Michael
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Maurer, Karl-Heinz
A1  - Jaeger, Karl-Erich
T1  - Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides
JF  - Applied and environmental microbiology
N2  - Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains.
Y1  - 2010
U6  - http://dx.doi.org/10.1128/AEM.01146-10
SN  - 1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)
VL  - 76
IS  - 19
SP  - 6370
EP  - 6378
PB  - American Society for Microbiology
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Werner, Frederik
A1  - Krumbe, Christoph
A1  - Schumacher, Katharina
A1  - Groebel, Simone
A1  - Spelthahn, Heiko
A1  - Stellberg, Michael
A1  - Wagner, Torsten
A1  - Yoshinobu, Tatsuo
A1  - Selmer, Thorsten
A1  - Keusgen, Michael
A1  - Baumann, Marcus
A1  - Schöning, Michael Josef
T1  - Determination of the extracellular acidification of Escherichia coli by a light-addressable potentiometric sensor
JF  - Physica status solidi (a) : applications and material science. 208 (2011), H. 6
Y1  - 2011
SN  - 1862-6319
SP  - 1340
EP  - 1344
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Martins, Berta M.
A1  - Blaser, Martin
A1  - Feliks, Mikolaj
A1  - Ullmann, Matthias G.
A1  - Buckel, Wolfgang
A1  - Selmer, Thorsten
T1  - Structural basis for a Kolbe-type decarboxylation catalyzed by a glycyl radical enzyme
JF  - Journal of the American Chemical Society
Y1  - 2011
N1  - Just accepted manuscript
SP  - 1
EP  - 33
PB  - ACS Publications
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Schiffels, Johannes
A1  - Baumann, Marcus
A1  - Selmer, Thorsten
T1  - Facile analysis of short-chain fatty acids as 4-nitrophenyl esters in complex anaerobic fermentation samples by high performance liquid chromatography
JF  - Journal of Chromatography A. 1218 (2011), H. 34
Y1  - 2011
SN  - 0021-9673
SP  - 5848
EP  - 5851
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Bäcker, Matthias
A1  - Delle, L.
A1  - Poghossian, Arshak
A1  - Biselli, Manfred
A1  - Zang, Werner
A1  - Wagner, P.
A1  - Schöning, Michael Josef
T1  - Electrochemical sensor array for bioprocess monitoring
JF  - Electrochimica Acta (2011)
Y1  - 2011
VL  - 56
IS  - 26
SP  - 9673
EP  - 9678
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Aggarwal, Prerna
A1  - Singh, Virendra
A1  - Singh, Arjun
A1  - Scherer, Ulrich W.
A1  - Singh, Tejvir
A1  - Singla, Madan L.
A1  - Srivastava, Alok
T1  - Physico-chemical transformations in swift heavy ion modified poly(ethyleneterephthalate)
JF  - Radiation Physics and Chemistry
Y1  - 2011
SN  - 0969-806X
N1  - In Press, Accepted Manuscript
SP  - 1
EP  - 28
PB  - Pergamon Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Oosterhuis, Koen
A1  - Öhlschläger, Peter
A1  - Berg, Joost H. van den
A1  - Toebes, Mireille
A1  - Gomez, Raquel
A1  - Schumacher, Ton N.
A1  - Haanen, John B.
T1  - Preclinical development of highly effective and safe DNA vaccines directed against HPV 16 E6 and E7
JF  - International Journal of Cancer
Y1  - 2011
SN  - 1097-0215
VL  - 129
IS  - 2
SP  - 397
EP  - 406
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Öhlschläger, Peter
A1  - Spies, Elmar
A1  - Alvarez, Gerardo
A1  - Quetting, Michael
A1  - Groettrup, Marcus
T1  - The combination of TLR-9 adjuvantation and electroporation-mediated delivery enhances in vivo antitumor responses after vaccination with HPV-16 E7 encoding DNA
JF  - International Journal of Cancer. 128 (2011), H. 2
Y1  - 2011
SN  - 1097-0215
SP  - 473
EP  - 481
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Manea, Marilena
A1  - Leurs, Ulrike
A1  - Orban, Erika
A1  - Baranyai, Zsuzsa
A1  - Öhlschläger, Peter
A1  - Marquardt, Andreas
A1  - Schulcz, Akos
A1  - Tejeda, Miguel
T1  - Enhanced Enzymatic Stability and Antitumor Activity of Daunorubicin-GnRH-III Bioconjugates Modified in Position 4
JF  - Bioconjugate Chemistry
Y1  - 2011
SN  - 1520-4812
VL  - 22
IS  - 7
SP  - 1320
EP  - 1329
PB  - ACS
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Spiess, Elmar
A1  - Wilfried, Reichardt
A1  - Alvarez, Gerardo
A1  - Gottrup, Marcus
A1  - Öhlschläger, Peter
T1  - An Artificial PAP Gene Breaks Self-tolerance and Promotes Tumor Regression in the TRAMP Model for Prostate Carcinoma
JF  - Molecular Therapy
Y1  - 2011
SN  - 1525-0016
VL  - 20
IS  - 3
SP  - 555
EP  - 564
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Deppe, Veronika Maria
A1  - Bongaerts, Johannes
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
A1  - Meinhardt, Friedhelm
T1  - Enzymatic deglycation of Amadori products in bacteria
JF  - Applied microbiology and biotechnology
Y1  - 2011
SN  - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)
VL  - Vol. 90
IS  - Iss. 2
SP  - 399
EP  - 406
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Deppe, Veronika Maria
A1  - Klatte, Stephanie
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - O'Connell, Timothy
A1  - Meinhardt, Friedhelm
T1  - Genetic control of Amadori product degradation in Bacillus subtilis via regulation of frlBONMD expression by FrlR
JF  - Applied and environmental microbiology
Y1  - 2011
SN  - 1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)
VL  - Vol. 77
IS  - No. 9
SP  - 2839
EP  - 2846
PB  - American Society of Mechanical Engineers (ASME)
CY  - New York
ER  - 
TY  - JOUR
A1  - Bongaerts, Johannes
A1  - Esser, Simon
A1  - Lorbach, Volker
A1  - Al-Momani, Lóay
A1  - Müller, Michael A.
A1  - Franke, Dirk
A1  - Grondal, Christoph
A1  - Kurutsch, Anja
A1  - Bujnicki, Robert
A1  - Takors, Ralf
A1  - Raeven, Leon
A1  - Wubbolts, Marcel
A1  - Bovenberg, Roel
A1  - Nieger, Martin
A1  - Schürmann, Melanie
A1  - Trachtmann, Natalie
A1  - Kozak, Stefan
A1  - Sprenger, Georg A.
A1  - Müller, Michael
T1  - Diversity-oriented production of metabolites derived from chorismate and their use in organic synthesis
JF  - Angewandte Chemie International Edition
Y1  - 2011
SN  - 1521-3773 (E-Journal); 0570-0833 (Print); 1433-7851 (Print)
VL  - Vol. 50
IS  - Iss. 34
SP  - 7781
EP  - 7786
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Niehaus, F.
A1  - Gabor, E.
A1  - Wieland, S.
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
A1  - Eck, J.
T1  - Enzymes for the laundry industries: tapping the vast metagenomic pool of alkaline proteases
JF  - Microbial biotechnology
Y1  - 2011
SN  - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)
VL  - Vol. 4
IS  - Iss. 6
SP  - 767
EP  - 776
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Raab, Monika
A1  - Kappel, Sven
A1  - Krämer, Andrea
A1  - Sanhaji, Mourad
A1  - Matthess, Yves
A1  - Kurunci-Csacsko, Elisabeth
A1  - Calzada-Wack, Julia
A1  - Rathkolb, Birgit
A1  - Rosman, Jan
A1  - Adler, Thure
A1  - Busch, Dirk H.
A1  - Esposito, Irene
A1  - Fuchs, Helmut
A1  - Gailus-Durner, Valérie
A1  - Klingenspor, Martin
A1  - Wolf, Eckhard
A1  - Sänger, Nicole
A1  - Prinz, Florian
A1  - Hrabe de Angelis, Martin
A1  - Seibler, Jost
A1  - Yuan, Juping
A1  - Bergmann, Martin
A1  - Knecht, Rainald
A1  - Kreft, Bertolt
A1  - Strebhardt, Klaus
T1  - Toxicity modelling of Plk1-targeted therapies in genetically engineered mice and cultured primary mammalian cells
JF  - Nature Communications
Y1  - 2011
U6  - http://dx.doi.org/10.1038/ncomms1395
SN  - 2041-1723
VL  - 2
IS  - 395
SP  - 1
EP  - 11
PB  - Nature
CY  - London
ER  - 
TY  - JOUR
A1  - Hasegawa, Maki
A1  - Kapelyukh, Yury
A1  - Tahara, Harunobu
A1  - Seibler, Jost
A1  - Rode, Anja
A1  - Krueger, Sylvia
A1  - Lee, Dongtao N.
A1  - Wolf, C. Roland
A1  - Scheer, Nico
T1  - Quantitative prediction of human pregnane X receptor and cytochrome P450 3A4 mediated drug-drug interaction in a novel multiple humanized mouse line
JF  - Molecular Pharmacology
Y1  - 2011
U6  - http://dx.doi.org/10.1124/mol.111.071845
SN  - 1521-0111
VL  - 80
IS  - 33
SP  - 518
EP  - 528
PB  - ASPET
CY  - Bethesda, Md.
ER  -