TY  - JOUR
A1  - Seifarth, Volker
A1  - Schehl, D.
A1  - Linder, Peter
A1  - Gossmann, Matthias
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Porst, Dariusz
A1  - Preiß, C.
A1  - Kayser, Peter
A1  - Pack, O.
A1  - Temiz Artmann, Aysegül
T1  - Ureplace: development of a bioreactor for in vitro culturing of cell seeded tubular vessels on collagen scaffolds
N2  - The demand of replacements for inoperable organs exceeds the amount of available organ transplants. Therefore, tissue engineering developed as a multidisciplinary field of research for autologous in-vitro organs. Such three dimensional tissue constructs request the application of a bioreactor. The UREPLACE bioreactor is used to grow cells on tubular collagen scaffolds OPTIMAIX Sponge 1 with a maximal length of 7 cm, in order to culture in vitro an adequate ureter replacement. With a rotating unit, (urothelial) cells can be placed homogeneously on the inner scaffold surface. Furthermore, a stimulation is combined with this bioreactor resulting in an orientation of muscle cells. These culturing methods request a precise control of several parameters and actuators. A combination of a LabBox and the suitable software LabVision is used to set and conduct parameters like rotation angles, velocities, pressures and other important cell culture values. The bioreactor was tested waterproof successfully. Furthermore, the temperature controlling was adjusted to 37 °C and the CO2 - concentration regulated to 5 %. Additionally, the pH step responses of several substances showed a perfect functioning of the designed flow chamber. All used software was tested and remained stable for several days.
KW  - Tissue Engineering
KW  - Bioreaktor
KW  - Organkultur
KW  - Harnleiter
Y1  - 2011
ER  - 
TY  - JOUR
A1  - Kurulgan Demirci, Eylem
A1  - Demirci, T.
A1  - Trzewik, Jürgen
A1  - Linder, Peter
A1  - Karakulah, G.
A1  - Artmann, Gerhard
A1  - Sakizli, M.
A1  - Temiz Artmann, Aysegül
T1  - Genome-Wide Gene Expression Analysis of NIH 3T3 Cell Line Under Mechanical Stimulation
JF  - Cellular and molecular bioengineering. 4 (2011), H. 1
Y1  - 2011
SN  - 1865-5025
SP  - 46
EP  - 55
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
T1  - The emperor's new body : seeking for a blueprint of limb regeneration in humans
JF  - Stem cell engineering : principles and applications / Gerhard M. Artmann ... eds.
Y1  - 2011
SN  - 978-3-642-11864-7
SP  - 3
EP  - 37
PB  - Springer
CY  - Berlin [u.a.]
ER  - 
TY  - JOUR
A1  - Zhubanova, Azhar A.
A1  - Aknazarov, S. K.
A1  - Mansurov, Zulkhair
A1  - Digel, Ilya
A1  - Kozhalakova, A. A.
A1  - Akimbekov, Nuraly Shardarbekovich
A1  - O'Heras, Carlos
A1  - Tazhibayeva, S.
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Adsorption of bacterial Lipopolysaccharides and blood plasma proteins on modified carbonized materials
N2  - Bacterial lipopolysaccharides (endotoxins) show strong biological effects at very low concentrations in human beings and many animals when entering the blood stream. These include affecting structure and function of organs and cells, changing metabolic functions, raising body temperature, triggering the coagulation cascade, modifying hemodynamics and causing septic shock. Because of this toxicity, the removal of even minute amounts is essential for safe parenteral administration of drugs and also for septic shock patients' care. The absence of a general method for endotoxin removal from liquid interfaces urgently requires finding new methods and materials to overcome this gap. Nanostructured carbonized plant parts is a promising material that showed good adsorption properties due to its vast pore network and high surface area. The aim of this study was comparative measurement of endotoxin- and blood proteins-related adsorption rate and adsorption capacity for different carboneous materials produced at different temperatures and under different surface modifications. As a main surface modificator, positively cbarged polymer, polyethileneimine (PEl) was used. Activated carbon materials showed good adsorption properties for LPS and some proteins used in the experiments. During the batch experiments, several techniques (dust removal, autoclaving) were used and optimized for improving the material's adsorption behavior. Also, with the results obtained it was possible to differentiate the materials according to their adsorption capacity and kinetic characteristics. Modification of the surface apparently has not affected hemoglobin binding to the adsorbent's surface. Obtained adsorption isotherms can be used as a powerful tool for designing of future column-based setups for blood purification from LPS, which is especially important for septic shock treatment.
KW  - Kohlenstofffaser
KW  - Lipopolysaccharide
KW  - nanostrukturierte carbonisierte Pflanzenteile
KW  - lipopolysaccharides
KW  - nanostructured carbonized plant parts
Y1  - 2010
ER  - 
TY  - JOUR
A1  - Leimena, W.
A1  - Artmann, Gerhard
A1  - Dachwald, Bernd
A1  - Temiz Artmann, Aysegül
A1  - Gossmann, Matthias
A1  - Digel, Ilya
T1  - Feasibility of an in-situ microbial decontamination of an ice-melting probe
JF  - Eurasian Chemico-Technological Journal. 12 (2010), H. 2
Y1  - 2010
SN  - 1562-3920
SP  - 145
EP  - 150
ER  - 
TY  - JOUR
A1  - Akimbekov, N. Sh.
A1  - Zhubanova, A. A.
A1  - Mansurov, Z. A.
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Use of Carbonized Rise Shell for the local treatment of wounds
JF  - Eurasian ChemTech Journal
N2  - On the model of musculocutaneous wound in rats, the effect of applicative sorption by carbonized rise shell (CRS) on the healing of festering wound was studied. It has been shown, that cytological changes end with rapid scar formation. The use of CRS at the period of severe purulent wound contributes to its favorable course, prevents the development of complications of the animals from sepsis.
Y1  - 2010
U6  - http://dx.doi.org/10.18321/ectj35
SN  - 2522-4867
VL  - 12
IS  - 2
SP  - 133
EP  - 138
PB  - Institute of Combustion Problems
CY  - Almaty
ER  - 
TY  - JOUR
A1  - Kozhalakova, A. A.
A1  - Zhubanova, Azhar A.
A1  - Mansurov, Z. A.
A1  - Digel, Ilya
A1  - Tazhibayeva, S. M.
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Adsorption of bacterial lipopolysaccharides on carbonized rice shell
JF  - Science of Central Asia (2010)
Y1  - 2010
SP  - 50
EP  - 54
ER  - 
TY  - JOUR
A1  - Kurz, R.
A1  - Linder, Peter
A1  - Trzewik, Jürgen
A1  - Rüffer, M.
A1  - Artmann, Gerhard
A1  - Digel, Ilya
A1  - Rothermel, A.
A1  - Robitzki, A.
A1  - Temiz Artmann, Aysegül
T1  - Contractile tension and beating rates of self-exciting monolayers and 3D-tissue constructs of neonatal rat cardiomyocytes
JF  - Medical and Biological Engineering and Computing
N2  - The CellDrum technology (The term 'CellDrum technology' includes a couple of slightly different technological setups for measuring lateral mechanical tension in various types of cell monolayers or 3D-tissue constructs) was designed to quantify the contraction rate and mechanical tension of self-exciting cardiac myocytes. Cells were grown either within flexible, circular collagen gels or as monolayer on top of respective 1-mum thin silicone membranes. Membrane and cells were bulged outwards by air pressure. This biaxial strain distribution is rather similar the beating, blood-filled heart. The setup allowed presetting the mechanical residual stress level externally by adjusting the centre deflection, thus, mimicking hypertension in vitro. Tension was measured as oscillating differential pressure change between chamber and environment. A 0.5-mm thick collagen-cardiac myocyte tissue construct induced after 2 days of culturing (initial cell density 2 x 10(4) cells/ml), a mechanical tension of 1.62 +/- 0.17 microN/mm(2). Mechanical load is an important growth regulator in the developing heart, and the orientation and alignment of cardiomyocytes is stress sensitive. Therefore, it was necessary to develop the CellDrum technology with its biaxial stress-strain distribution and defined mechanical boundary conditions. Cells were exposed to strain in two directions, radially and circumferentially, which is similar to biaxial loading in real heart tissues. Thus, from a biomechanical point of view, the system is preferable to previous setups based on uniaxial stretching.
Y1  - 2010
U6  - http://dx.doi.org/10.1007/s11517-009-0552-y
SN  - 1741-0444
VL  - 48
IS  - 1
SP  - 59
EP  - 65
PB  - Springer Nature
CY  - Cham
ER  - 
TY  - JOUR
A1  - Artmann, Gerhard
A1  - Digel, Ilya
A1  - Zerlin, Kay
A1  - Maggakis-Kelemen, Christina
A1  - Linder, Peter
A1  - Porst, Dariusz
A1  - Kayser, Peter
A1  - Stadler, David
A1  - Dikta, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Hemoglobin senses body temperature
JF  - European Biophysics Journal
Y1  - 2009
SN  - 0175-7571
VL  - 38
IS  - 5
SP  - 589
EP  - 600
ER  - 
TY  - JOUR
A1  - Sadykov, Rustam
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
A1  - Porst, Dariusz
A1  - Linder, Peter
A1  - Kayser, Peter
A1  - Artmann, Gerhard
A1  - Savitskaya, Irina
A1  - Zhubanova, Azhar
T1  - Oral lead exposure induces dysbacteriosis in rats
JF  - Journal of Occupational Health. 51 (2009) (2009), H. 1
Y1  - 2009
SN  - 1348-9585
SP  - 64
EP  - 73
ER  - 
TY  - JOUR
A1  - Demirci, Taylan
A1  - Kurulgan Demirci, Eylem
A1  - Trzewik, Jürgen
A1  - Linder, Peter
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Sakizli, Meral
A1  - Temiz Artmann, Aysegül
T1  - Gene expression profile analysis of 3T3/NIH fibroblasts after one hour mechanical stress
JF  - IUBMB Life. 61 (2009), H. 3
Y1  - 2009
SN  - 1521-6543
N1  - Abstracts: Turkish Society of Molecular Medicine, Third International Congress of Molecular Medicine, May 5-8, 2009, Istanbul, Turkey
SP  - 311
EP  - 312
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Amin, Rashid
A1  - Temiz Artmann, Aysegül
A1  - Artmann, Gerhard
A1  - Lazarovici, Philip
A1  - Lelkes, Peter I.
T1  - Permeability of an In Vitro Model of Blood Brain Barrier (BBB)
JF  - 13th International Conference on Biomedical Engineering / Lim, Chwee Teck [Ed.]
Y1  - 2009
SN  - 978-3-540-92841-6
N1  - IFMBE Proceedings ; 23, Track 1 ; ICBME 2008 3–6 December 2008 Singapore
SP  - 81
EP  - 84
ER  - 
TY  - JOUR
A1  - Kurulgan Demirci, Eylem
A1  - Linder, Peter
A1  - Demirci, Taylan
A1  - Trzewik, Jürgen
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Contractile tension of endothelial cells: An LPS based in-vitro sepsis model
JF  - IUBMB Life. 61 (2009), H. 3
Y1  - 2009
SN  - 1521-6543
N1  - Abstracts: Turkish Society of Molecular Medicine, Third International Congress of Molecular Medicine, May 5-8, 2009, Istanbul, Turkey
SP  - 307
EP  - 308
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Kayser, Peter
T1  - Why is Sepsis an Ongoing Clinical Challenge? Lipopolysaccharide Effects on Red Blood Cell Volume / Temiz, Aysegül ;  Kayser, Peter
JF  - Bioengineering in Cell and Tissue Research / Artmann, Gerhard M. ; Chien, Shu (Eds.)
Y1  - 2008
SN  - 978-3-540-75408-4
SP  - 497
EP  - 508
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Linder, Peter
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
A1  - Kayser, Peter
A1  - Porst, Dariusz
A1  - Artmann, Gerhard
T1  - High-throughput testing of mechanical forces generated in thin cell and tissue layers
JF  - Tissue Engineering. 13 (2007), H. 7
Y1  - 2007
SN  - 1076-3279
N1  - Meeting abstract 433
SP  - 1778
EP  - 1778
ER  - 
TY  - JOUR
A1  - Erbayraktar, Zubeyde
A1  - Yilmaz, Osman
A1  - Temiz Artmann, Aysegül
A1  - Cehreli, Ruksan
A1  - Coker, Canan
T1  - Effects of Selenium Supplementation on Antioxidant Defense and Glucose Homeostasis in Experimental Diabetes Mellitus
JF  - Biological Trace Element Research
Y1  - 2007
SN  - 1559-0720
VL  - 118
IS  - 3
SP  - 217
EP  - 226
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Zerlin, Kay
A1  - Temiz Artmann, Aysegül
A1  - Engels, S.
T1  - Protein dynamics in thermosensation
JF  - Regenerative medicine. 2 (2007), H. 5
Y1  - 2007
SN  - 1746-0751
N1  - Proceedings of the 3rd World Congress on Regenerative Medicine. October 18-20, 2007. Leipzig, Germany
SP  - 533
EP  - 533
ER  - 
TY  - JOUR
A1  - Zerlin, Kay
A1  - Kasischke, Nicole
A1  - Digel, Ilya
A1  - Maggakis-Kelemen, Christina
A1  - Temiz Artmann, Aysegül
A1  - Porst, Dariusz
A1  - Kayser, Peter
A1  - Linder, Peter
A1  - Artmann, Gerhard
T1  - Structural transition temperature of hemoglobins correlates with species’ body temperature
JF  - European Biophysics Journal. 37 (2007), H. 1
Y1  - 2007
SN  - 1432-1017
SP  - 1
EP  - 10
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Linder, Peter
A1  - Kayser, Peter
A1  - Digel, Ilya
T1  - NMR in vitro effects on proliferation, apoptosis, and viability of human chondrocytes and osteoblasts
JF  - Methods and findings in Experimental and Clinical Pharmacology. 27 (2005), H. 6
Y1  - 2005
SN  - 0379-0355
SP  - 391
EP  - 394
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Kayatekin, Muammer B.
A1  - Uysal, N.
A1  - Resmi, H.
T1  - Does antioxidant supplementation alter the effects of acute exercise on erythrocyte aggregation, deformability and endothelium adhesion in untrained rats? Kayatekin, Muammer B.; Uysal N.; Resmi, H.; Bediz, Seref C.; Temiz Artmann, A.; Genç, S.; Tugyan, K.
JF  - International Journal for Vitamin and Nutrition Research. 75 (2005), H. 4
Y1  - 2005
SN  - 0300-9831
SP  - 243
EP  - 250
ER  -