TY - JOUR A1 - Ribitsch, D. A1 - Heumann, S. A1 - Karl, W. A1 - Gerlach, J. A1 - Leber, R. A1 - Birner-Gruenberger, R. A1 - Gruber, K. A1 - Eiteljoerg, I. A1 - Remler, P. A1 - Siegert, Petra A1 - Lange, J. A1 - Maurer, Karl-Heinz A1 - Berg, G. A1 - Guebitz, G. M. A1 - Schwab, H. T1 - Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli JF - Journal of biotechnology N2 - A large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. Several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. Encapsulation of cells in alginate beads induced protease production. Stenotrophomonas maltophilia emerged as best performer under washing conditions. For identification of wash-active proteases, four extracellular serine proteases called StmPr1, StmPr2, StmPr3 and StmPr4 were cloned. StmPr2 and StmPr4 were sufficiently overexpressed in E. coli. Expression of StmPr1 and StmPr3 resulted in unprocessed, insoluble protein. Truncation of most of the C-terminal domain which has been identified by enzyme modeling succeeded in expression of soluble, active StmPr1 but failed in case of StmPr3. From laundry application tests StmPr2 turned out to be a highly wash-active protease at 45 °C. Specific activity of StmPr2 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 17 ± 2 U/mg. In addition we determined the kinetic parameters and cleavage preferences of protease StmPr2. KW - Alginate beads KW - Stenotrophomonas maltophilia KW - Detergent protease Y1 - 2012 U6 - https://doi.org/10.1016/j.jbiotec.2011.09.025 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 157 IS - 1 SP - 140 EP - 147 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Schmitz, Philipp A1 - Weber, Martin T1 - Buying and selling behavior of individual investors in option-like securities JF - Die Betriebswirtschaft : DBW N2 - We analyze the trading behavior of individual investors in option-like securities, namely bankissued warrants, and thus expand the growing literature of investors behavior to a new kind of securities. A unique data set from a large German discount broker gives us the opportunity to analyze the trading behavior of 1,454 investors, making 89,958 transactions in 6,724 warrants on 397 underlyings. In different logit regression, we make use of the facts that investors can speculate on rising and falling prices of the underlying with call and put warrants and that we also have information about the stock portfolios of the investors. We report several facts about the trading behavior of individual investors in warrants that are consistent with the literature on the behavior of individual investors in the stock market. The warrant investors buy calls and sell puts if the price of the underlying has decreased over the past trading days and they sell calls and buy puts if the price of the underlying has increased. That means, the investors follow negative feedback trading strategies in all four trading categories observed. In addition, we find strong evidence for the disposition effect for call as well as put warrants, which is reversed in December. The trading behavior is also influenced if the underlying reaches some exceptionally prices, e.g. highs, lows or the strike price. We show that hedging, as one natural candidate to buy puts, does not play an important role in the market for bank-issued warrants. KW - Bank-issued Warrants KW - Disposition Effect KW - Negative Feedback Trading KW - Trading Behavior KW - Individual Investors Y1 - 2012 U6 - https://doi.org/10.2139/ssrn.2055088 SN - 0342-7064 VL - 72 IS - 5 SP - 409 EP - 426 PB - Schäffer-Poeschel CY - Stuttgart ER - TY - JOUR A1 - Schmitz, Philipp A1 - Weber, Martin T1 - Buying and selling behavior of individual investors in option-like securities Y1 - 2007 SP - 1 EP - 36 ER - TY - JOUR A1 - Glaser, Markus A1 - Schmitz, Philipp T1 - Privatanleger am Optionsscheinmarkt JF - Zeitschrift für Bankrecht und Bankwirtschaft : ZBB Y1 - 2007 SN - 0936-2800 N1 - Printausg. in der Bibliothek Eupener Str. vorhanden VL - Jg. 19 IS - H. 3 SP - 214 EP - 230 ER - TY - JOUR A1 - Glaser, Markus A1 - Schmitz, Philipp T1 - Privatanleger am Optionsscheinmarkt Y1 - 2006 SP - 1 EP - 46 ER - TY - JOUR A1 - Meiners, Klaus A1 - Baumann, Marcus T1 - Lokale Beiträge zum globalen Klimaschutz JF - Wechselwirkung : Wissenschaft & vernetztes Denken Y1 - 1997 SN - 0172-1623 VL - 19 IS - 84 SP - 6 EP - 12 ER - TY - JOUR A1 - Baumann, Marcus A1 - Gronsfeld, A. A1 - Schütz-Lembach, G. T1 - Nachdenklichkeit über Nachhaltigkeit : wie uns ein neues Wort alte Probleme neu entdecken läßt JF - Wechselwirkung : Wissenschaft & vernetztes Denken Y1 - 1997 SN - 0172-1623 VL - 19 IS - 87 SP - 6 EP - 11 ER - TY - JOUR A1 - Baumann, Marcus A1 - Peschel, G. T1 - Aktuelle Entwicklungen zur Luftreinhaltung in der Stadt Aachen JF - Wechselwirkung : Wissenschaft & vernetztes Denken Y1 - 1998 SN - 0172-1623 VL - 20 IS - 91 SP - 22 EP - 29 ER - TY - JOUR A1 - Baumann, Marcus A1 - Peschel, G. T1 - Das Klima Aachens, lokale Ansprüche und globale Auswirkungen JF - Wechselwirkung : Wissenschaft & vernetztes Denken Y1 - 1998 SN - 0172-1623 VL - 20 IS - 93 SP - 26 EP - 33 ER - TY - JOUR A1 - Wiezorek, E. A1 - Baumann, Marcus T1 - Stadtökologie in der Stadtplanung: der "Stadtökologische Beitrag" Aachen JF - Wechselwirkung : Wissenschaft & vernetztes Denken Y1 - 1999 SN - 0172-1623 VL - 21 IS - 97 SP - 28 EP - 37 ER - TY - JOUR A1 - Ribitsch, D. A1 - Heumann, S. A1 - Trotscha, E. A1 - Herrero Acero, E. A1 - Greimel, K. A1 - Leber, R. A1 - Birger-Gruenberger, R. A1 - Deller, S. A1 - Eiteljoerg, I. A1 - Remler, P. A1 - Weber, Th. A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Donelli, I. A1 - Freddi, G. A1 - Schwab, H. A1 - Guebitz, G. M. T1 - Hydrolysis of polyethyleneterephthalate by p-nitrobenzylesterase from Bacillus subtilis JF - Biotechnology progress Y1 - 2011 SN - 1520-6033 (E-Journal); 8756-7938 (Print) VL - Vol. 27 IS - Iss. 4 SP - 951 EP - 960 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Ribitsch, D. A1 - Karl, W. A1 - Birner-Gruenberger, R. A1 - Gruber, K. A1 - Eiteljoerg, I. A1 - Remler, P. A1 - Wieland, S. A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schwab, H. T1 - C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli JF - Journal of biotechnology N2 - Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7–11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease. Y1 - 2010 U6 - https://doi.org/10.1016/j.jbiotec.2010.09.947 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 150 IS - 3 SP - 408 EP - 416 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Siegert, Petra A1 - McLeish, Michael J. A1 - Baumann, Martin A1 - Iding, Hans A1 - Kneen, Malea M. A1 - Kenyon, George L. A1 - Pohl, Martina T1 - Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida JF - Protein engineering, design, and selection : peds Y1 - 2005 SN - 1460-213X (E-Journal); 1741-0134 (E-Journal); 0269-2139 (Print); 1741-0126 (Print) VL - Vol. 18 IS - Iss. 7 SP - 345 EP - 357 ER - TY - JOUR A1 - Dünkelmann, Pascal A1 - Kolter-Jung, Doris A1 - Nitsche, Adam A1 - Demir, Ayhan S. A1 - Siegert, Petra A1 - Lingen, Bettina A1 - Baumann, Martin A1 - Pohl, Martina A1 - Müller, Michael T1 - Development of a donor-acceptor concept for enzymatic cross-coupling reactions of adehydes : the first asymmetric cross-benzoin condensation JF - Journal of the American Chemical Society Y1 - 2002 SN - 1520-5126 (E-Journal); 0002-7863 (Print) VL - Vol. 124 SP - 12084 EP - 12085 ER - TY - JOUR A1 - Dünnwald, Thomas A1 - Demir, Ayhan S. A1 - Siegert, Petra A1 - Pohl, Martina A1 - Müller, Michael T1 - ChemInform Abstract: Enantioselective synthesis of (S)-2-Hydroxypropanone derivatives by Benzoylformate Decarboxylase Catalyzed C—C Bond Formation JF - Cheminform Y1 - 2001 SN - 1522-2667 (E-Journal); 0931-7597 (Print) VL - Vol. 32 IS - Iss. 4 SP - Publ. online ER - TY - JOUR A1 - Dünnwald, Thomas A1 - Demir, Ayhan S. A1 - Siegert, Petra A1 - Pohl, Martina A1 - Müller, Michael T1 - Enantioselective Synthesis of (S)-2-Hydroxypropanone Derivatives by Benzoylformate Decarboxylase Catalyzed C−C Bond Formation JF - European journal of organic chemistry Y1 - 2000 SN - 0365-5490 (E-Journal); 1099-0690 (E-Journal); 0075-4617 (Print); 0170-2041 (Print); 0947-3440 (Print); 1434-193X (Print); 1434-243X (Print) VL - Vol. 2000 IS - Iss. 11 SP - 2161 EP - 2170 ER - TY - JOUR A1 - Iding, Hans A1 - Dünnwald, Thomas A1 - Greiner, Lasse A1 - Liese, Andreas A1 - Müller, Michael A1 - Siegert, Petra A1 - Grötzinger, Joachim A1 - Demir, Ayhan S. A1 - Pohl, Martina T1 - Benzoylformate Decarboxylase from Pseudomonas putida as Stable Catalyst for the Synthesis of Chiral 2-Hydroxy Ketones JF - Chemistry - a European journal Y1 - 2000 SN - 1521-3765 (E-Journal); 0947-6539 (Print) VL - Vol. 6 IS - Iss. 8 SP - 1483 EP - 1495 ER - TY - JOUR A1 - Alebouyeh Samami, Behzad A1 - Pieper, Martin A1 - Breitbach, Gerd A1 - Hodapp, Josef T1 - Heat production in the windings of the stators of electric machines under stationary condition JF - Heat and mass transfer N2 - In electric machines due to high currents and resistive losses (joule heating) heat is produced. To avoid damages by overheating the design of effective cooling systems is required. Therefore the knowledge of heat sources and heat transfer processes is necessary. The purpose of this paper is to illustrate a good and effective calculation method for the temperature analysis based on homogenization techniques. These methods have been applied for the stator windings in a slot of an electric machine consisting of copper wires and resin. The key quantity here is an effective thermal conductivity, which characterizes the heterogeneous wire resin-arrangement inside the stator slot. To illustrate the applicability of the method, the analysis of a simplified, homogenized model is compared with the detailed analysis of temperature behavior inside a slot of an electric machine according to the heat generation. We considered here only the stationary situation. The achieved numerical results are accurate and show that the applied homogenization technique works in practice. Finally the results of simulations for the two cases, the original model of the slot and the homogenized model chosen for the slot (unit cell), are compared to experimental results. Y1 - 2014 U6 - https://doi.org/10.1007/s00231-014-1371-8 SN - 0947-7411 (Print) ; 1432-1181 (E-Journal) VL - 50 SP - 1707 EP - 1716 PB - Springer CY - Heidelberg ER - TY - JOUR A1 - Guo, Yuanyuan A1 - Seki, Kosuke A1 - Miyamoto, Ko-ichiro A1 - Wagner, Torsten A1 - Schöning, Michael Josef A1 - Yoshinobu, Tatsuo T1 - Novel photoexcitation method for light-addressable potentiometric sensor with higher spatial resolution JF - Applied physics express : APEX N2 - A novel photoexcitation method for the light-addressable potentiometric sensor (LAPS) is proposed to achieve a higher spatial resolution of chemical images. The proposed method employs a combined light source that consists of a modulated light probe, which generates the alternating photocurrent signal, and a ring of constant illumination surrounding it. The constant illumination generates a sheath of carriers with increased concentration which suppresses the spread of photocarriers by enhanced recombination. A device simulation was carried out to verify the effect of constant illumination on the spatial resolution, which demonstrated that a higher spatial resolution can be obtained. Y1 - 2014 U6 - https://doi.org/10.7567/APEX.7.067301 SN - 1882-0786 (E-Journa); 1882-0778 (Print) VL - 7 IS - 6 SP - 067301-4 PB - IOP CY - Bristol ER - TY - JOUR A1 - Whitehead, Mark A1 - Öhlschläger, Peter A1 - Almajhdi, Fahad N. A1 - Alloza, Leonor A1 - Marzábal, Pablo A1 - Meyers, Ann E. A1 - Hitzeroth, Inga I. A1 - Rybicki, Edward P. T1 - Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice JF - BMC cancer Y1 - 2014 U6 - https://doi.org/10.1186/1471-2407-14-367 SN - 1471-2407 IS - 14:367 SP - 1 EP - 15 PB - BioMed Central CY - London ER -