TY - JOUR A1 - Monakhova, Yulia A1 - Diehl, Bernd W.K. T1 - Multinuclear NMR screening of pharmaceuticals using standardization by 2H integral of a deuterated solvent JF - Journal of Pharmaceutical and Biomedical Analysis N2 - NMR standardization approach that uses the 2H integral of deuterated solvent for quantitative multinuclear analysis of pharmaceuticals is described. As a proof of principle, the existing NMR procedure for the analysis of heparin products according to US Pharmacopeia monograph is extended to the determination of Na+ and Cl- content in this matrix. Quantification is performed based on the ratio of a 23Na (35Cl) NMR integral and 2H NMR signal of deuterated solvent, D2O, acquired using the specific spectrometer hardware. As an alternative, the possibility of 133Cs standardization using the addition of Cs2CO3 stock solution is shown. Validation characteristics (linearity, repeatability, sensitivity) are evaluated. A holistic NMR profiling of heparin products can now also be used for the quantitative determination of inorganic compounds in a single analytical run using a single sample. In general, the new standardization methodology provides an appealing alternative for the NMR screening of inorganic and organic components in pharmaceutical products. KW - NMR spectroscopy KW - Inorganic ions KW - Heparin KW - Standardization Y1 - 2022 SN - 0731-7085 U6 - https://doi.org/10.1016/j.jpba.2021.114530 VL - 209 IS - Article number: 114530 PB - Elsevier ER - TY - JOUR A1 - Block, Franziska A1 - May, Alexander A1 - Wetzel, Katharina A1 - Adels, Klaudia A1 - Elbers, Gereon A1 - Schulze, Margit A1 - Monakhova, Yulia T1 - What is the best spectroscopic method for simultaneous analysis of organic acids and (poly)saccharides in biological matrices: Example of Aloe vera extracts? JF - Talanta Open N2 - Several species of (poly)saccharides and organic acids can be found often simultaneously in various biological matrices, e.g., fruits, plant materials, and biological fluids. The analysis of such matrices sometimes represents a challenging task. Using Aloe vera (A. vera) plant materials as an example, the performance of several spectro-scopic methods (80 MHz benchtop NMR, NIR, ATR-FTIR and UV–vis) for the simultaneous analysis of quality parameters of this plant material was compared. The determined parameters include (poly)saccharides such as aloverose, fructose and glucose as well as organic acids (malic, lactic, citric, isocitric, acetic, fumaric, benzoic and sorbic acids). 500 MHz NMR and high-performance liquid chromatography (HPLC) were used as the reference methods. UV–vis data can be used only for identification of added preservatives (benzoic and sorbic acids) and drying agent (maltodextrin) and semiquantitative analysis of malic acid. NIR and MIR spectroscopies combined with multivariate regression can deliver more informative overview of A. vera extracts being able to additionally quantify glucose, aloverose, citric, isocitric, malic, lactic acids and fructose. Low-field NMR measurements can be used for the quantification of aloverose, glucose, malic, lactic, acetic, and benzoic acids. The benchtop NMR method was successfully validated in terms of robustness, stability, precision, reproducibility and limit of detection (LOD) and quantification (LOQ), respectively. All spectroscopic techniques are useful for the screening of (poly)saccharides and organic acids in plant extracts and should be applied according to its availability as well as information and confidence required for the specific analytical goal. Benchtop NMR spectroscopy seems to be the most feasible solution for quality control of A. vera products. KW - Spectroscopy KW - (Poly)saccharides KW - Organic acids KW - Aloe vera KW - qNMR Y1 - 2023 U6 - https://doi.org/10.1016/j.talo.2023.100220 SN - 2666-8319 VL - 7 IS - Art. No. 100220 SP - 1 EP - 9 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Molinnus, Denise A1 - Janus, Kevin Alexander A1 - Fang, Anyelina C. A1 - Drinic, Aleksander A1 - Achtsnicht, Stefan A1 - Köpf, Marius A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Thick-film carbon electrode deposited onto a biodegradable fibroin substrate for biosensing applications JF - Physica status solidi (a) N2 - This study addresses a proof-of-concept experiment with a biocompatible screen-printed carbon electrode deposited onto a biocompatible and biodegradable substrate, which is made of fibroin, a protein derived from silk of the Bombyx mori silkworm. To demonstrate the sensor performance, the carbon electrode is functionalized as a glucose biosensor with the enzyme glucose oxidase and encapsulated with a silicone rubber to ensure biocompatibility of the contact wires. The carbon electrode is fabricated by means of thick-film technology including a curing step to solidify the carbon paste. The influence of the curing temperature and curing time on the electrode morphology is analyzed via scanning electron microscopy. The electrochemical characterization of the glucose biosensor is performed by amperometric/voltammetric measurements of different glucose concentrations in phosphate buffer. Herein, systematic studies at applied potentials from 500 to 1200 mV to the carbon working electrode (vs the Ag/AgCl reference electrode) allow to determine the optimal working potential. Additionally, the influence of the curing parameters on the glucose sensitivity is examined over a time period of up to 361 days. The sensor shows a negligible cross-sensitivity toward ascorbic acid, noradrenaline, and adrenaline. The developed biocompatible biosensor is highly promising for future in vivo and epidermal applications. KW - biocompatible materials KW - biodegradable electronic devices KW - biosensors KW - carbon electrodes KW - glucose Y1 - 2022 U6 - https://doi.org/10.1002/pssa.202200100 SN - 1862-6319 N1 - Corresponding author: Michael J. Schöning VL - 219 IS - 23 SP - 1 EP - 9 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Özsoylu, Dua A1 - Kizildag, Sefa A1 - Schöning, Michael Josef A1 - Wagner, Torsten T1 - Differential chemical imaging of extracellular acidification within microfluidic channels using a plasma-functionalized light-addressable potentiometric sensor (LAPS) JF - Physics in Medicine N2 - Extracellular acidification is a basic indicator for alterations in two vital metabolic pathways: glycolysis and cellular respiration. Measuring these alterations by monitoring extracellular acidification using cell-based biosensors such as LAPS plays an important role in studying these pathways whose disorders are associated with numerous diseases including cancer. However, the surface of the biosensors must be specially tailored to ensure high cell compatibility so that cells can represent more in vivo-like behavior, which is critical to gain more realistic in vitro results from the analyses, e.g., drug discovery experiments. In this work, O2 plasma patterning on the LAPS surface is studied to enhance surface features of the sensor chip, e.g., wettability and biofunctionality. The surface treated with O2 plasma for 30 s exhibits enhanced cytocompatibility for adherent CHO–K1 cells, which promotes cell spreading and proliferation. The plasma-modified LAPS chip is then integrated into a microfluidic system, which provides two identical channels to facilitate differential measurements of the extracellular acidification of CHO–K1 cells. To the best of our knowledge, it is the first time that extracellular acidification within microfluidic channels is quantitatively visualized as differential (bio-)chemical images. Y1 - 2020 U6 - https://doi.org/10.1016/j.phmed.2020.100030 SN - 2352-4510 VL - 10 IS - 100030 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Engelmann, Ulrich M. A1 - Pourshahidi, Mohammad Ali A1 - Shalaby, Ahmed A1 - Krause, Hans-Joachim T1 - Probing particle size dependency of frequency mixing magnetic detection with dynamic relaxation simulation JF - Journal of Magnetism and Magnetic Materials N2 - Biomedical applications of magnetic nanoparticles (MNP) fundamentally rely on the particles’ magnetic relaxation as a response to an alternating magnetic field. The magnetic relaxation complexly depends on the interplay of MNP magnetic and physical properties with the applied field parameters. It is commonly accepted that particle core size is a major contributor to signal generation in all the above applications, however, most MNP samples comprise broad distribution spanning nm and more. Therefore, precise knowledge of the exact contribution of individual core sizes to signal generation is desired for optimal MNP design generally for each application. Specifically, we present a magnetic relaxation simulation-driven analysis of experimental frequency mixing magnetic detection (FMMD) for biosensing to quantify the contributions of individual core size fractions towards signal generation. Applying our method to two different experimental MNP systems, we found the most dominant contributions from approx. 20 nm sized particles in the two independent MNP systems. Additional comparison between freely suspended and immobilized MNP also reveals insight in the MNP microstructure, allowing to use FMMD for MNP characterization, as well as to further fine-tune its applicability in biosensing. Y1 - 2022 U6 - https://doi.org/10.1016/j.jmmm.2022.169965 SN - 0304-8853 VL - 563 IS - In progress, Art. No. 169965 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Achtsnicht, Stefan A1 - Tödter, Julia A1 - Niehues, Julia A1 - Telöken, Matthias A1 - Offenhäusser, Andreas A1 - Krause, Hans-Joachim A1 - Schröper, Florian T1 - 3D printed modular immunofiltration columns for frequency mixing-based multiplex magnetic immunodetection JF - Sensors N2 - For performing point-of-care molecular diagnostics, magnetic immunoassays constitute a promising alternative to established enzyme-linked immunosorbent assays (ELISA) because they are fast, robust and sensitive. Simultaneous detection of multiple biomolecular targets from one body fluid sample is desired. The aim of this work is to show that multiplex magnetic immunodetection based on magnetic frequency mixing by means of modular immunofiltration columns prepared for different targets is feasible. By calculations of the magnetic response signal, the required spacing between the modules was determined. Immunofiltration columns were manufactured by 3D printing and antibody immobilization was performed in a batch approach. It was shown experimentally that two different target molecules in a sample solution could be individually detected in a single assaying step with magnetic measurements of the corresponding immobilization filters. The arrangement order of the filters and of a negative control did not influence the results. Thus, a simple and reliable approach to multi-target magnetic immunodetection was demonstrated. Y1 - 2019 U6 - https://doi.org/10.3390/s19010148 SN - 1424-8220 VL - 19 IS - 1 PB - MDPI CY - Basel ER - TY - JOUR A1 - Heuermann, Holger A1 - Sadeghfam, Arash T1 - Enhanced system architecture for rugged wide band data transmission / Sadeghfam, A. ; Heuermann, H. JF - European Radar Conference, 2009 : EuRAD 2009 ; Sept. 30 - Oct. 2 2009, Rome, Italy ; part of the European Microwave Week (EuMW) / sponsored by EuMA, European Microwave Association Y1 - 2009 SN - 978-2-87487-014-9 N1 - Proceedings of the 6th European Radar Conference SP - 347 EP - 350 PB - IEEE CY - Piscataway, NJ ER - TY - JOUR A1 - Adels, Klaudia A1 - Elbers, Gereon A1 - Diehl, Bernd A1 - Monakhova, Yulia T1 - Multicomponent analysis of dietary supplements containing glucosamine and chondroitin: comparative low- and high-field NMR spectroscopic study JF - Analytical Sciences N2 - With the prevalence of glucosamine- and chondroitin-containing dietary supplements for people with osteoarthritis in the marketplace, it is important to have an accurate and reproducible analytical method for the quantitation of these compounds in finished products. NMR spectroscopic method based both on low- (80 MHz) and high- (500–600 MHz) field NMR instrumentation was established, compared and validated for the determination of chondroitin sulfate and glucosamine in dietary supplements. The proposed method was applied for analysis of 20 different dietary supplements. In the majority of cases, quantification results obtained on the low-field NMR spectrometer are similar to those obtained with high-field 500–600 MHz NMR devices. Validation results in terms of accuracy, precision, reproducibility, limit of detection and recovery demonstrated that the developed method is fit for purpose for the marketed products. The NMR method was extended to the analysis of methylsulfonylmethane, adulterant maltodextrin, acetate and inorganic ions. Low-field NMR can be a quicker and cheaper alternative to more expensive high-field NMR measurements for quality control of the investigated dietary supplements. High-field NMR instrumentation can be more favorable for samples with complex composition due to better resolution, simultaneously giving the possibility of analysis of inorganic species such as potassium and chloride. KW - Glucosamine KW - Chondroitin sulfate KW - Polysaccharides KW - Dietary supplements KW - High-field NMR Y1 - 2023 U6 - https://doi.org/10.1007/s44211-023-00433-2 SN - 1348-2246 (Online) SN - 0910-6340 (Print) N1 - Corresponding author: Yulia Monakhova VL - 2023 PB - Springer Verlag CY - Cham ER - TY - JOUR A1 - Trapp, Svenja A1 - Lammers, Tom A1 - Engudar, Gokce A1 - Hoehr, Cornelia A1 - Denkova, Antonia G. A1 - Paulßen, Elisabeth A1 - de Kruijff, Robin M. T1 - Membrane-based microfluidic solvent extraction of Ga-68 from aqueous Zn solutions: towards an automated cyclotron production loop JF - EJNMMI Radiopharmacy and Chemistry KW - Microfluidic solvent extraction KW - Ga-68 KW - Cyclotron production KW - Medical radionuclide production KW - Metal contaminants Y1 - 2023 U6 - https://doi.org/10.1186/s41181-023-00195-2 SN - 2365-421X VL - 2023 IS - 8, Article number: 9 SP - 1 EP - 14 PB - Springer Nature ER - TY - JOUR A1 - Monakhova, Yulia A1 - Diehl, Bernd W. K. T1 - A step towards optimization of the qNMR workflow: proficiency testing exercise at an GxP-accredited laboratory JF - Applied Magnetic Resonance N2 - Quantitative nuclear magnetic resonance (qNMR) is considered as a powerful tool for multicomponent mixture analysis as well as for the purity determination of single compounds. Special attention is currently paid to the training of operators and study directors involved in qNMR testing. To assure that only qualified personnel are used for sample preparation at our GxP-accredited laboratory, weighing test was proposed. Sixteen participants performed six-fold weighing of the binary mixture of dibutylated hydroxytoluene (BHT) and 1,2,4,5-tetrachloro-3-nitrobenzene (TCNB). To evaluate the quality of data analysis, all spectra were evaluated manually by a qNMR expert and using in-house developed automated routine. The results revealed that mean values are comparable and both evaluation approaches are free of systematic error. However, automated evaluation resulted in an approximately 20% increase in precision. The same findings were revealed for qNMR analysis of 32 compounds used in pharmaceutical industry. Weighing test by six-fold determination in binary mixtures and automated qNMR methodology can be recommended as efficient tools for evaluating staff proficiency. The automated qNMR method significantly increases throughput and precision of qNMR for routine measurements and extends application scope of qNMR. Y1 - 2021 U6 - https://doi.org/10.1007/s00723-021-01324-3 SN - 1613-7507 N1 - Corresponding author: Yulia Monakhova VL - 52 SP - 581 EP - 593 PB - Springer Nature CY - Wien ER - TY - JOUR A1 - Engelmann, Ulrich M. A1 - Shalaby, Ahmed A1 - Shasha, Carolyn A1 - Krishnan, Kannan M. A1 - Krause, Hans-Joachim T1 - Comparative modeling of frequency mixing measurements of magnetic nanoparticles using micromagnetic simulations and Langevin theory JF - Nanomaterials N2 - Dual frequency magnetic excitation of magnetic nanoparticles (MNP) enables enhanced biosensing applications. This was studied from an experimental and theoretical perspective: nonlinear sum-frequency components of MNP exposed to dual-frequency magnetic excitation were measured as a function of static magnetic offset field. The Langevin model in thermodynamic equilibrium was fitted to the experimental data to derive parameters of the lognormal core size distribution. These parameters were subsequently used as inputs for micromagnetic Monte-Carlo (MC)-simulations. From the hysteresis loops obtained from MC-simulations, sum-frequency components were numerically demodulated and compared with both experiment and Langevin model predictions. From the latter, we derived that approximately 90% of the frequency mixing magnetic response signal is generated by the largest 10% of MNP. We therefore suggest that small particles do not contribute to the frequency mixing signal, which is supported by MC-simulation results. Both theoretical approaches describe the experimental signal shapes well, but with notable differences between experiment and micromagnetic simulations. These deviations could result from Brownian relaxations which are, albeit experimentally inhibited, included in MC-simulation, or (yet unconsidered) cluster-effects of MNP, or inaccurately derived input for MC-simulations, because the largest particles dominate the experimental signal but concurrently do not fulfill the precondition of thermodynamic equilibrium required by Langevin theory. KW - Magnetic nanoparticles KW - Frequency mixing magnetic detection KW - Langevin theory KW - Micromagnetic simulation KW - Nonequilibrium dynamics Y1 - 2021 SN - 2079-4991 U6 - https://doi.org/10.3390/nano11051257 N1 - This article belongs to the Special Issue Applications and Properties of Magnetic Nanoparticles VL - 11 IS - 5 SP - 1 EP - 16 PB - MDPI CY - Basel ER - TY - JOUR A1 - Aliazizi, Fereshteh A1 - Özsoylu, Dua A1 - Bakhshi Sichani, Soroush A1 - Khorshid, Mehran A1 - Glorieux, Christ A1 - Robbens, Johan A1 - Schöning, Michael Josef A1 - Wagner, Patrick T1 - Development and Calibration of a Microfluidic, Chip-Based Sensor System for Monitoring the Physical Properties of Water Samples in Aquacultures JF - Micromachines N2 - In this work, we present a compact, bifunctional chip-based sensor setup that measures the temperature and electrical conductivity of water samples, including specimens from rivers and channels, aquaculture, and the Atlantic Ocean. For conductivity measurements, we utilize the impedance amplitude recorded via interdigitated electrode structures at a single triggering frequency. The results are well in line with data obtained using a calibrated reference instrument. The new setup holds for conductivity values spanning almost two orders of magnitude (river versus ocean water) without the need for equivalent circuit modelling. Temperature measurements were performed in four-point geometry with an on-chip platinum RTD (resistance temperature detector) in the temperature range between 2 °C and 40 °C, showing no hysteresis effects between warming and cooling cycles. Although the meander was not shielded against the liquid, the temperature calibration provided equivalent results to low conductive Milli-Q and highly conductive ocean water. The sensor is therefore suitable for inline and online monitoring purposes in recirculating aquaculture systems. KW - chip-based sensor setup KW - aquaculture KW - microfluidics KW - impedance spectroscopy KW - thermometry KW - electrical conductivity of liquids Y1 - 2024 U6 - https://doi.org/10.3390/mi15060755 SN - 2072-666X N1 - This article belongs to the Special Issue "Multisensor Arrays" N1 - Corresponding author: Michael J. Schöning VL - 15 IS - 6 PB - MDPI CY - Basel ER - TY - JOUR A1 - Kurz, Melanie T1 - Der Modell-Roller JF - Form : the making of design Y1 - 2006 SN - 0015-7678 VL - 2006 IS - 209 SP - 64 EP - 72 ER - TY - JOUR A1 - Zhantlessova, Sirina A1 - Savitskaya, Irina A1 - Kistaubayeva, Aida A1 - Ignatova, Ludmila A1 - Talipova, Aizhan A1 - Pogrebnjak, Alexander A1 - Digel, Ilya T1 - Correction: Zhantlessova et al. advanced “Green” prebiotic composite of bacterial cellulose/pullulan based on synthetic biology-powered microbial coculture strategy. Polymers 2022, 14, 3224 JF - Polymers Y1 - 2024 U6 - https://doi.org/10.3390/polym16131802 SN - 2073-4360 N1 - This article belongs to the Special Issue Cellulose Based Composites VL - 16 IS - 13 PB - MDPI CY - Basel ER - TY - JOUR A1 - Falkenberg, Fabian A1 - Voß, Leonie A1 - Bott, Michael A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - New robust subtilisins from halotolerant and halophilic Bacillaceae JF - Applied Microbiology and Biotechnology N2 - The aim of the present study was the characterisation of three true subtilisins and one phylogenetically intermediate subtilisin from halotolerant and halophilic microorganisms. Considering the currently growing enzyme market for efficient and novel biocatalysts, data mining is a promising source for novel, as yet uncharacterised enzymes, especially from halophilic or halotolerant Bacillaceae, which offer great potential to meet industrial needs. Both halophilic bacteria Pontibacillus marinus DSM 16465ᵀ and Alkalibacillus haloalkaliphilus DSM 5271ᵀ and both halotolerant bacteria Metabacillus indicus DSM 16189 and Litchfieldia alkalitelluris DSM 16976ᵀ served as a source for the four new subtilisins SPPM, SPAH, SPMI and SPLA. The protease genes were cloned and expressed in Bacillus subtilis DB104. Purification to apparent homogeneity was achieved by ethanol precipitation, desalting and ion-exchange chromatography. Enzyme activity could be observed between pH 5.0–12.0 with an optimum for SPPM, SPMI and SPLA around pH 9.0 and for SPAH at pH 10.0. The optimal temperature for SPMI and SPLA was 70 °C and for SPPM and SPAH 55 °C and 50 °C, respectively. All proteases showed high stability towards 5% (w/v) SDS and were active even at NaCl concentrations of 5 M. The four proteases demonstrate potential for future biotechnological applications. KW - Biotechnological application KW - Bacillaceae KW - Subtilisin KW - Subtilases KW - Halotolerant protease Y1 - 2023 U6 - https://doi.org/10.1007/s00253-023-12553-w SN - 1432-0614 N1 - Corresponding author: Petra Siegert VL - 107 SP - 3939 EP - 3954 PB - Springer Nature CY - Berlin ER - TY - JOUR A1 - Saretzki, Charlotte A1 - Bergmann, Ole A1 - Dahmann, Peter A1 - Janser, Frank A1 - Keimer, Jona A1 - Machado, Patricia A1 - Morrison, Audry A1 - Page, Henry A1 - Pluta, Emil A1 - Stübing, Felix A1 - Küpper, Thomas T1 - Are small airplanes safe with regards to COVID-19 transmission? JF - Journal of Travel Medicine Y1 - 2021 U6 - https://doi.org/10.1093/jtm/taab105 SN - 1708-8305 VL - 28 IS - 7 PB - Oxford University Press CY - Oxford ER - TY - JOUR A1 - Harris, Isaac A1 - Kleefeld, Andreas T1 - Analysis and computation of the transmission eigenvalues with a conductive boundary condition JF - Applicable Analysis N2 - We provide a new analytical and computational study of the transmission eigenvalues with a conductive boundary condition. These eigenvalues are derived from the scalar inverse scattering problem for an inhomogeneous material with a conductive boundary condition. The goal is to study how these eigenvalues depend on the material parameters in order to estimate the refractive index. The analytical questions we study are: deriving Faber–Krahn type lower bounds, the discreteness and limiting behavior of the transmission eigenvalues as the conductivity tends to infinity for a sign changing contrast. We also provide a numerical study of a new boundary integral equation for computing the eigenvalues. Lastly, using the limiting behavior we will numerically estimate the refractive index from the eigenvalues provided the conductivity is sufficiently large but unknown. KW - Boundary integral equations KW - Inverse spectral problem KW - Conductive boundary condition KW - Transmission eigenvalues Y1 - 2020 U6 - https://doi.org/10.1080/00036811.2020.1789598 SN - 1563-504X VL - 101 IS - 6 SP - 1880 EP - 1895 PB - Taylor & Francis CY - London ER - TY - JOUR A1 - Bähr, Brigitte T1 - [Gert Hoepner] im Interview zum Thema ; Verpackungspolitik - mehr als Hülle JF - Packaging journal : Technologie, Logistik, Marketing, Produkte Y1 - 2012 SN - 1610-0336 IS - 5 SP - 78 EP - 80 PB - Ella Verlag CY - Hürth ER - TY - JOUR A1 - Kuperjans, Isabel T1 - Exergetische und exergoökonomische Analyse thermischer Prozesse JF - Arbeitsbericht / Institut für Wirtschaftswissenschaften, Rheinisch-Westfälische Technische Hochschule Aachen Y1 - 1996 IS - 06 ER - TY - JOUR A1 - Haeger, Gerrit A1 - Wirges, Jessika A1 - Tanzmann, Nicole A1 - Oyen, Sven A1 - Jolmes, Tristan A1 - Jaeger, Karl-Erich A1 - Schörken, Ulrich A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - Chaperone assisted recombinant expression of a mycobacterial aminoacylase in Vibrio natriegens and Escherichia coli capable of N-lauroyl-L-amino acid synthesis JF - Microbial Cell Factories N2 - Background Aminoacylases are highly promising enzymes for the green synthesis of acyl-amino acids, potentially replacing the environmentally harmful Schotten-Baumann reaction. Long-chain acyl-amino acids can serve as strong surfactants and emulsifiers, with application in cosmetic industries. Heterologous expression of these enzymes, however, is often hampered, limiting their use in industrial processes. Results We identified a novel mycobacterial aminoacylase gene from Mycolicibacterium smegmatis MKD 8, cloned and expressed it in Escherichia coli and Vibrio natriegens using the T7 overexpression system. The recombinant enzyme was prone to aggregate as inclusion bodies, and while V. natriegens Vmax™ could produce soluble aminoacylase upon induction with isopropyl β-d-1-thiogalactopyranoside (IPTG), E. coli BL21 (DE3) needed autoinduction with lactose to produce soluble recombinant protein. We successfully conducted a chaperone co-expression study in both organisms to further enhance aminoacylase production and found that overexpression of chaperones GroEL/S enhanced aminoacylase activity in the cell-free extract 1.8-fold in V. natriegens and E. coli. Eventually, E. coli ArcticExpress™ (DE3), which co-expresses cold-adapted chaperonins Cpn60/10 from Oleispira antarctica, cultivated at 12 °C, rendered the most suitable expression system for this aminoacylase and exhibited twice the aminoacylase activity in the cell-free extract compared to E. coli BL21 (DE3) with GroEL/S co-expression at 20 °C. The purified aminoacylase was characterized based on hydrolytic activities, being most stable and active at pH 7.0, with a maximum activity at 70 °C, and stability at 40 °C and pH 7.0 for 5 days. The aminoacylase strongly prefers short-chain acyl-amino acids with smaller, hydrophobic amino acid residues. Several long-chain amino acids were fairly accepted in hydrolysis as well, especially N-lauroyl-L-methionine. To initially evaluate the relevance of this aminoacylase for the synthesis of N-acyl-amino acids, we demonstrated that lauroyl-methionine can be synthesized from lauric acid and methionine in an aqueous system. Conclusion Our results suggest that the recombinant enzyme is well suited for synthesis reactions and will thus be further investigated. KW - Acyl-amino acids KW - Inclusion bodies KW - Chaperone co-expression KW - Vibrio natriegens KW - Aminoacylase Y1 - 2023 U6 - https://doi.org/10.1186/s12934-023-02079-1 SN - 1475-2859 N1 - Corresponding author: Petra Siegert IS - 22 SP - Article number: 77 (2023) PB - Springer Nature ER -