TY - JOUR A1 - Spelthahn, Heiko A1 - Schubert, Jürgen A1 - Schöning, Michael Josef T1 - Dünnschichtsensoren für die Schwermetallanalytik JF - GIT : Labor-Fachzeitschrift N2 - Die Detektion von Schadstoffen repräsentiert in der Umweltanalytik eine wichtige Aufgabenstellung. Gerade die Abwasser- bzw. Brauchwasseranalytik sowie die Prozesskontrolle haben einen hohen Stellenwert. Siliziumbasierte Dünnschichtsensoren bieten eine kostengünstige Möglichkeit, „online“-Messungen bzw. Vor-Ort-Messungen zeitnah durchzuführen. In dieser Arbeit wird ein potentiometrisches Sensorarray auf der Basis von Chalkogenidgläsern zur Detektion von Schwermetallen in wässrigen Medien vorgestellt. Y1 - 2012 SN - 0016-3538 VL - 56 IS - 4 SP - 285 EP - 287 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Spelthahn, Heiko A1 - Schubert, Jürgen A1 - Schöning, Michael Josef T1 - Dünnschichtsensoren für die Schwermetallanalytik : Mikroelektroden auf Chalkogenidglasbasis JF - GIT Labor-Fachzeitschrift N2 - Die Detektion von Schadstoffen repräsentiert in der Umweltanalytik eine wichtige Aufgabenstellung. Gerade die Abwasser- bzw. Brauchwasseranalytik sowie die Prozesskontrolle haben einen hohen Stellenwert. Siliziumbasierte Dünnschichtsensoren bieten eine kostengünstige Möglichkeit, „online“-Messungen bzw. Vor-Ort-Messungen zeitnah durchzuführen. In dieser Arbeit wird ein potentiometrisches Sensorarray auf der Basis von Chalkogenidgläsern zur Detektion von Schwermetallen in wässrigen Medien vorgestellt. Y1 - 2012 IS - 4 SP - 285 EP - 287 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Bassam, Rasha A1 - Hescheler, Jürgen A1 - Temiz Artmann, Aysegül A1 - Artmann, Gerhard A1 - Digel, Ilya T1 - Effects of spermine NONOate and ATP on the thermal stability of hemoglobin JF - BMC Biophysics N2 - Background Minor changes in protein structure induced by small organic and inorganic molecules can result in significant metabolic effects. The effects can be even more profound if the molecular players are chemically active and present in the cell in considerable amounts. The aim of our study was to investigate effects of a nitric oxide donor (spermine NONOate), ATP and sodium/potassium environment on the dynamics of thermal unfolding of human hemoglobin (Hb). The effect of these molecules was examined by means of circular dichroism spectrometry (CD) in the temperature range between 25°C and 70°C. The alpha-helical content of buffered hemoglobin samples (0.1 mg/ml) was estimated via ellipticity change measurements at a heating rate of 1°C/min. Results Major results were: 1) spermine NONOate persistently decreased the hemoglobin unfolding temperature T u irrespectively of the Na + /K + environment, 2) ATP instead increased the unfolding temperature by 3°C in both sodium-based and potassium-based buffers and 3) mutual effects of ATP and NO were strongly influenced by particular buffer ionic compositions. Moreover, the presence of potassium facilitated a partial unfolding of alpha-helical structures even at room temperature. Conclusion The obtained data might shed more light on molecular mechanisms and biophysics involved in the regulation of protein activity by small solutes in the cell. KW - Nitric Oxide Donor KW - NONOate KW - Circular Dichroism KW - Nitric Oxide Y1 - 2012 U6 - http://dx.doi.org/10.1186/2046-1682-5-16 SN - 2046-1682 VL - 5 PB - BioMed Central CY - London ER - TY - JOUR A1 - Bragard, G. A1 - Bragard, Michael T1 - Ein kostengünstiges, universelles Modellbauinterface JF - Der mathematische und naturwissenschaftliche Unterricht : MNU ; Organ des Deutschen Vereins zur Förderung des Mathematischen und Naturwissenschaftlichen Unterrichts e.V. N2 - Wir stellen einen USB-Baustein vor, der eine kostengünstige und universelle Möglichkeit schafft , im Unterricht den Themenkreis Messen-Steuern-Regeln zu behandeln. Die Funktionalität orientiert sich am CVK-Interface der Firma Fischertechnik. Im Gegensatz zu kommerziellen Lösungen erlaubt unser Aufbau auch den preiswerten Einsatz in Gruppen- oder Einzelarbeit. Abschließend berichten wir über ein Beispiel aus dem Unterrichtseinsatz. Y1 - 2012 SN - 0025-5866 VL - 65 IS - 5 SP - 290 EP - 293 PB - Seeberger CY - Neuss ER - TY - JOUR A1 - Kroll-Ludwigs, Kathrin T1 - Ein optionales Vertragsrecht für Europa: Motor oder Hemmnis für den Binnenmarkt? JF - GPR : Zeitschrift für Gemeinschaftsprivatrecht Y1 - 2018 U6 - http://dx.doi.org/10.1515/gpr.2012.9.4.181 SN - 2193-9519 VL - 9 IS - 4 SP - 181 EP - 188 PB - Verlag Dr. Otto Schmidt CY - Köln ER - TY - JOUR A1 - Olaru, Alexandra Maria A1 - Kowalski, Julia A1 - Sethi, Vaishali A1 - Blümich, Bernhard T1 - Exchange relaxometry of flow at small Péclet numbers in a glass bead pack JF - Journal of Magnetic Resonance (JMR) N2 - In this paper we consider low Péclet number flow in bead packs. A series of relaxation exchange experiments has been conducted and evaluated by ILT analysis. In the resulting correlation maps, we observed a collapse of the signal and a translation towards smaller relaxation times with increasing flow rates, as well as a signal tilt with respect to the diagonal. In the discussion of the phenomena we present a mathematical theory for relaxation exchange experiments that considers both diffusive and advective transport. We perform simulations based on this theory and discuss them with respect to the conducted experiments. KW - NMR exchange relaxometry KW - Low-field NMR Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.jmr.2012.04.015 SN - 1096-0856 VL - 220 SP - 32 EP - 44 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Fateri, Miranda A1 - Hötter, Jan-Steffen A1 - Gebhardt, Andreas T1 - Experimental and Theoretical Investigation of Buckling Deformation of Fabricated Objects by Selective Laser Melting JF - Physics Procedia N2 - Although Selective Laser Melting (SLM) process is an innovative manufacturing method, there are challenges such as inferior mechanical properties of fabricated objects. Regarding this, buckling deformation which is caused by thermal stress is one of the undesired mechanical properties which must be alleviated. As buckling deformation is more observable in hard to process materials, silver is selected to be studied theoretically and experimentally for this paper. Different scanning strategies are utilized and a Finite Element Method (FEM) is applied to calculate the temperature gradient in order to determine its effect on the buckling deformation of the objects from experiments. Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.phpro.2012.10.062 SN - 1875-3892 N1 - Part of special issue "Laser Assisted Net shape Engineering 7 (LANE 2012)" VL - 39 SP - 464 EP - 470 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Schneider, Bettina A1 - Schneider, Wilhelm T1 - Externes Rechnungswesen nach BilMoG: Grundelemente und Aufbau JF - Das Wirtschaftsstudium : wisu ; Zeitschrift für Ausbildung, Examen, Berufseinstieg und Fortbildung Y1 - 2012 SN - 0340-3084 VL - 41 IS - 3 SP - 332 EP - 337 PB - Lange CY - Düsseldorf ER - TY - JOUR A1 - Ribitsch, D. A1 - Heumann, S. A1 - Karl, W. A1 - Gerlach, J. A1 - Leber, R. A1 - Birner-Gruenberger, R. A1 - Gruber, K. A1 - Eiteljoerg, I. A1 - Remler, P. A1 - Siegert, Petra A1 - Lange, J. A1 - Maurer, Karl-Heinz A1 - Berg, G. A1 - Guebitz, G. M. A1 - Schwab, H. T1 - Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli JF - Journal of biotechnology N2 - A large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. Several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. Encapsulation of cells in alginate beads induced protease production. Stenotrophomonas maltophilia emerged as best performer under washing conditions. For identification of wash-active proteases, four extracellular serine proteases called StmPr1, StmPr2, StmPr3 and StmPr4 were cloned. StmPr2 and StmPr4 were sufficiently overexpressed in E. coli. Expression of StmPr1 and StmPr3 resulted in unprocessed, insoluble protein. Truncation of most of the C-terminal domain which has been identified by enzyme modeling succeeded in expression of soluble, active StmPr1 but failed in case of StmPr3. From laundry application tests StmPr2 turned out to be a highly wash-active protease at 45 °C. Specific activity of StmPr2 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 17 ± 2 U/mg. In addition we determined the kinetic parameters and cleavage preferences of protease StmPr2. KW - Alginate beads KW - Stenotrophomonas maltophilia KW - Detergent protease Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.jbiotec.2011.09.025 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 157 IS - 1 SP - 140 EP - 147 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Poghossian, Arshak A1 - Weil, M. H. A1 - Bäcker, Matthias A1 - Mayer, D. A1 - Schöning, Michael Josef T1 - Field-effect Devices Functionalised with Gold-Nanoparticle/Macromolecule Hybrids: New Opportunities for a Label-Free Biosensing JF - Procedia Engineering N2 - Field-effect capacitive electrolyte-insulator-semiconductor (EIS) sensors functionalised with citrate-capped gold nanoparticles (AuNP) have been used for the electrostatic detection of macromolecules by their intrinsic molecular charge. The EIS sensor detects the charge changes in the AuNP/macromolecule hybrids induced by the adsorption or binding events. A feasibility of the proposed detection scheme has been exemplary demonstrated by realising EIS sensors for the detection of poly-D-lysine molecules. Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.proeng.2012.09.136 SN - 1877-7058 N1 - Part of special issue "26th European Conference on Solid-State Transducers, EUROSENSOR 2012" IS - 47 SP - 273 EP - 276 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Novacek, V. A1 - Tran, Thanh Ngoc A1 - Klinge, U. A1 - Tolba, R. H. A1 - Staat, Manfred A1 - Bronson, D. G. A1 - Miesse, A. M. A1 - Whiffen, J. A1 - Turquier, F. T1 - Finite element modelling of stapled colorectal end-to-end anastomosis : Advantages of variable height stapler design JF - Journal of Biomechanics N2 - The impact of surgical staplers on tissues has been studied mostly in an empirical manner. In this paper, finite element method was used to clarify the mechanics of tissue stapling and associated phenomena. Various stapling modalities and several designs of circular staplers were investigated to evaluate the impact of the device on tissues and mechanical performance of the end-to-end colorectal anastomosis. Numerical simulations demonstrated that a single row of staples is not adequate to resist leakage due to non-linear buckling and opening of the tissue layers between two adjacent staples. Compared to the single staple row configuration, significant increase in stress experienced by the tissue at the inner staple rows was observed in two and three rows designs. On the other hand, adding second and/or third staple row had no effect on strain in the tissue inside the staples. Variable height design with higher staples in outer rows significantly reduced the stresses and strains in outer rows when compared to the same configuration with flat cartridge. KW - Variable height stapler design KW - Anastomotic leakage KW - Finite element modelling KW - End-to-end colorectal anastomosis KW - Surgical staplers Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.jbiomech.2012.07.021 SN - 1873-2380 VL - 45 IS - 115 SP - 2693 EP - 2697 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Biselli, Manfred A1 - Selmer, Thorsten A1 - Öhlschläger, Peter A1 - Baumann, Marcus A1 - Förster, Arnold A1 - Poghossian, Arshak T1 - Forschung „zwischen“ den Disziplinen: das Institut für Nano- und Biotechnologien JF - Analytik news : das Online-Labormagazin für Labor und Analytik N2 - "Biologie trifft Mikroelektronik", das Motto des Instituts für Nano- und Biotechnologien (INB) an der FH Aachen, unterstreicht die zunehmende Bedeutung interdisziplinär geprägter Forschungsaktivitäten. Der thematische Zusammenschluss grundständiger Disziplinen, wie die Physik, Elektrotechnik, Chemie, Biologie sowie die Materialwissenschaften, lässt neue Forschungsgebiete entstehen, ein herausragendes Beispiel hierfür ist die Nanotechnologie: Hier werden neue Werkstoffe und Materialien entwickelt, einzelne Nanopartikel oder Moleküle und deren Wechselwirkung untersucht oder Schichtstrukturen im Nanometerbereich aufgebaut, die neue und vorher nicht bekannte Eigenschaften hervorbringen. Vor diesem Hintergrund bündelt das im Jahre 2006 gegründete INB die an der FH Aachen vorhandenen Kompetenzen von derzeit insgesamt sieben Laboratorien auf den Gebieten der Halbleitertechnik und Nanoelektronik, Nanostrukturen und DNA-Sensorik, der Chemo- und Biosensorik, der Enzymtechnologie, der Mikrobiologie und Pflanzenbiotechnologie, der Zellkulturtechnik, sowie der Roten Biotechnologie synergetisch. In der Nano- und Biotechnologie steckt außergewöhnliches Potenzial! Nicht zuletzt deshalb stellen sich die Forscher der Herausforderung, in diesem Bereich gemeinsam zu forschen und Schnittstellen zu nutzen, um so bei der Gestaltung neuartiger Ideen und Produkte mitzuwirken, die zukünftig unser alltägliches Leben verändern werden. Im Folgenden werden die verschiedenen Forschungsbereiche kurz zusammenfassend vorgestellt und vorhandene Interaktionen anhand von exemplarisch ausgewählten, aktuellen Forschungsprojekten skizziert. Y1 - 2012 VL - Publ. online PB - Dr. Beyer Internet-Beratung CY - Ober-Ramstadt ER - TY - JOUR A1 - Becker, Jörg A1 - Delfmann, Patrick A1 - Eggert, Mathias A1 - Schwittay, Sebastian T1 - Generalizability and Applicability of Model-Based Business Process Compliance-Checking Approaches — A State-of-the-Art Analysis and Research Roadmap JF - Business Research : BuR N2 - With a steady increase of regulatory requirements for business processes, automation support of compliance management is a field garnering increasing attention in Information Systems research. Several approaches have been developed to support compliance checking of process models. One major challenge for such approaches is their ability to handle different modeling techniques and compliance rules in order to enable widespread adoption and application. Applying a structured literature search strategy, we reflect and discuss compliance-checking approaches in order to provide an insight into their generalizability and evaluation. The results imply that current approaches mainly focus on special modeling techniques and/or a restricted set of types of compliance rules. Most approaches abstain from real-world evaluation which raises the question of their practical applicability. Referring to the search results, we propose a roadmap for further research in model-based business process compliance checking. Y1 - 2012 U6 - http://dx.doi.org/10.1007/BF03342739 SN - 1866-8658 VL - 5 IS - 2 SP - 221 EP - 247 PB - Springer CY - Heidelberg ER - TY - JOUR A1 - Scheer, Nico A1 - Balimane, Praveen A1 - Hayward, Michael D. A1 - Buechel, Sandra A1 - Kauselmann, Gunther A1 - Wolf, C. Roland T1 - Generation and Characterization of a Novel Multidrug Resistance Protein 2 Humanized Mouse Line JF - Drug Metabolism and Disposition N2 - The multidrug resistance protein (MRP) 2 is predominantly expressed in liver, intestine, and kidney, where it plays an important role in the excretion of a range of drugs and their metabolites or endogenous compounds into bile, feces, and urine. Mrp knockout [Mrp2(−/−)] mice have been used recently to study the role of MRP2 in drug disposition. Here, we describe the first generation and initial characterization of a mouse line humanized for MRP2 (huMRP2), which is nulled for the mouse Mrp2 gene and expresses the human transporter in the organs and cell types where MRP2 is normally expressed. Analysis of the mRNA expression for selected cytochrome P450 and transporter genes revealed no major changes in huMRP2 mice compared with wild-type controls. We show that human MRP2 is able to compensate functionally for the loss of the mouse transporter as demonstrated by comparable bilirubin levels in the humanized mice and wild-type controls, in contrast to the hyperbilirubinemia phenotype that is observed in MRP2(−/−) mice. The huMRP2 mouse provides a model to study the role of the human transporter in drug disposition and in assessing the in vivo consequences of inhibiting this transporter by compounds interacting with human MRP2. Y1 - 2012 U6 - http://dx.doi.org/10.1124/dmd.112.047605 SN - 1521-0111 VL - 40 IS - 11 SP - 2212 EP - 2218 PB - ASPET CY - Bethesda, Md. ER - TY - JOUR A1 - Scheer, Nico A1 - Kapelyukh, Yury A1 - Rode, Anja A1 - Buechel, Sandra A1 - Wolf, C. Roland T1 - Generation and characterization of novel cytochrome P450 Cyp2c gene cluster knockout and CYP2C9 humanized mouse lines JF - Molecular Pharmacology N2 - Compared with rodents and many other animal species, the human cytochrome P450 (P450) Cyp2c gene cluster varies significantly in the multiplicity of functional genes and in the substrate specificity of its enzymes. As a consequence, the use of wild-type animal models to predict the role of human CYP2C enzymes in drug metabolism and drug-drug interactions is limited. Within the human CYP2C cluster CYP2C9 is of particular importance, because it is one of the most abundant P450 enzymes in human liver, and it is involved in the metabolism of a wide variety of important drugs and environmental chemicals. To investigate the in vivo functions of cytochrome P450 Cyp2c genes and to establish a model for studying the functions of CYP2C9 in vivo, we have generated a mouse model with a deletion of the murine Cyp2c gene cluster and a corresponding humanized model expressing CYP2C9 specifically in the liver. Despite the high number of functional genes in the mouse Cyp2c cluster and the reported roles of some of these proteins in different biological processes, mice deleted for Cyp2c genes were viable and fertile but showed certain phenotypic alterations in the liver. The expression of CYP2C9 in the liver also resulted in viable animals active in the metabolism and disposition of a number of CYP2C9 substrates. These mouse lines provide a powerful tool for studying the role of Cyp2c genes and of CYP2C9 in particular in drug disposition and as a factor in drug-drug interaction. Y1 - 2012 U6 - http://dx.doi.org/10.1124/mol.112.080036 SN - 1521-0111 VL - 82 IS - 6 SP - 1022 EP - 1029 PB - ASPET CY - Bethesda, Md. ER - TY - JOUR A1 - Borgmeier, Claudia A1 - Bongaerts, Johannes A1 - Meinhardt, Friedhelm T1 - Genetic analysis of the Bacillus licheniformis degSU operon and the impact of regulatory mutations on protease production JF - Journal of biotechnology N2 - Disruption experiments targeted at the Bacillus licheniformis degSU operon and GFP-reporter analysis provided evidence for promoter activity immediately upstream of degU. pMutin mediated concomitant introduction of the degU32 allele – known to cause hypersecretion in Bacillus subtilis – resulted in a marked increase in protease activity. Application of 5-fluorouracil based counterselection through establishment of a phosphoribosyltransferase deficient Δupp strain eventually facilitated the marker-free introduction of degU32 leading to further protease enhancement achieving levels as for hypersecreting wild strains in which degU was overexpressed. Surprisingly, deletion of rapG – known to interfere with DegU DNA-binding in B. subtilis – did not enhance protease production neither in the wild type nor in the degU32 strain. The combination of degU32 and Δupp counterselection in the type strain is not only equally effective as in hypersecreting wild strains with respect to protease production but furthermore facilitates genetic strain improvement aiming at biological containment and effectiveness of biotechnological processes. KW - Marker-free mutagenesis KW - Extracellular enzymes KW - Uracil-phosphoribosyltransferase KW - Hypersecretion Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.jbiotec.2012.02.011 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 159 IS - 1-2 SP - 12 EP - 20 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Schneider, Bettina A1 - Schneider, Wilhelm T1 - Grundlagen der Unternehmensbesteuerung JF - Das Wirtschaftsstudium : wisu ; Zeitschrift für Ausbildung, Examen, Berufseinstieg und Fortbildung Y1 - 2012 SN - 0340-3084 VL - 41 IS - 10 SP - 1312 EP - 1318 PB - Lange CY - Düsseldorf ER - TY - JOUR A1 - Grinsven, Bart van A1 - Bon, Natalie vanden A1 - Strauven, Hannelore A1 - Grieten, Lars A1 - Murib, Mohammed A1 - Jiménez Monroy, Kathia L. A1 - Janssens, Stoffel D. A1 - Haenen, Ken A1 - Schöning, Michael Josef A1 - Vermeeren, Veronique A1 - Ameloot, Marcel A1 - Michiels, Luc A1 - Thoelen, Ronald A1 - Ceuninck, Ward de A1 - Wagner, Patrick T1 - Heat-Transfer Resistance at Solid-Liquid Interfaces: A Tool for The Detection of Single Nucleotide Polymorphisms in DNA. JF - ACS Nano N2 - In this article, we report on the heat-transfer resistance at interfaces as a novel, denaturation-based method to detect single-nucleotide polymorphisms in DNA. We observed that a molecular brush of double-stranded DNA grafted onto synthetic diamond surfaces does not notably affect the heat-transfer resistance at the solid-to-liquid interface. In contrast to this, molecular brushes of single-stranded DNA cause, surprisingly, a substantially higher heat-transfer resistance and behave like a thermally insulating layer. This effect can be utilized to identify ds-DNA melting temperatures via the switching from low- to high heat-transfer resistance. The melting temperatures identified with this method for different DNA duplexes (29 base pairs without and with built-in mutations) correlate nicely with data calculated by modeling. The method is fast, label-free (without the need for fluorescent or radioactive markers), allows for repetitive measurements, and can also be extended toward array formats. Reference measurements by confocal fluorescence microscopy and impedance spectroscopy confirm that the switching of heat-transfer resistance upon denaturation is indeed related to the thermal on-chip denaturation of DNA. Y1 - 2012 U6 - http://dx.doi.org/10.1021/nn300147e SN - 1936-086X VL - 6 IS - 3 SP - 2712 EP - 2721 PB - ACS Publications CY - Washington, DC ER - TY - JOUR A1 - Werner, Frederik A1 - Wagner, Torsten A1 - Miyamoto, Ko-ichiro A1 - Yoshinobu, Tatsuo A1 - Schöning, Michael Josef T1 - High speed and high resolution chemical imaging based on a new type of OLED-LAPS set-up JF - Sensors and Actuators B: Chemical N2 - Light-addressable potentiometric sensors (LAPS) are field-effect-based sensors. A modulated light source is used to define the particular measurement spot to perform spatially resolved measurements of chemical species and to generate chemical images. In this work, an organic-LED (OLED) display has been chosen as a light source. This allows high measurement resolution and miniaturisation of the system. A new developed driving method for the OLED display optimised for LAPS-based measurements is demonstrated. The new method enables to define modulation frequencies between 1 kHz and 16 kHz and hence, reduces the measurement time of a chemical image by a factor of 40 compared to the traditional addressing of an OLED display. Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.snb.2011.12.102 SN - 0925-4005 N1 - Part of special issue "Selected Papers presented at Eurosensors XXV" VL - 175 SP - 118 EP - 122 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Butenweg, Christoph A1 - Fehling, Ekkehard T1 - Hintergrund für die vereinfachten Regeln bei Mauerwerksgebäuden im Erdbebenfall JF - Mauerwerk : European journal of masonry N2 - Bei der Ausarbeitung des nationalen Anwendungsdokumentes zur DIN EN 1998-1 waren die in der ENV-Fassung enthaltenen vereinfachten Regeln im Lichte aktueller Forschungsergebnisse zu überprüfen und zu überarbeiten. Die gleiche Aufgabe stellte sich auch für die Neufassung der DIN 4149. In beiden Fällen sind neben konstruktiven Regeln für die Art und Anordnung der zur Gebäudeaussteifung heranzuziehenden Wände im Grundriss Tabellen enthalten, die unter bestimmten Bedingungen den Entfall eines rechnerischen Nachweises der Tragwände im Erdbebenfall ermöglichen. Dies ist für Schwachbebengebiete, wie sie in Deutschland und anderen Ländern Mitteleuropas anzutreffen sind, sinnvoll, um unnötigen Rechenaufwand sowie Probleme mit der Führbarkeit von Nachweisen so weit wie möglich auszuschalten. Im vorliegenden Beitrag werden die Hintergründe der vereinfachten Regeln diskutiert und die Ergebnisse der Anwendung mit verschiedenen Rechenverfahren verglichen und bewertet. Y1 - 2012 U6 - http://dx.doi.org/10.1002/dama.201200537 SN - 1437-1022 (E-Journal); 1432-3427 (Print) VL - Volume 16 IS - Issue 3 SP - 127 EP - 137 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Kirchhof, Michael A1 - Kraft, Bodo T1 - Hybrides Vorgehensmodell : Agile und klassische Methoden im Projekt passend kombinieren JF - ProjektMagazin N2 - Agil ist im Trend und immer mehr Unternehmen, die ihre Projekte bisher nach klassischen Prinzipien durchführten, denken über den Einsatz agiler Methoden nach. Doch selbst wenn die Organisation bereits beide Philosophien unterstützt, gilt für ein Projekt meist die klare Vorgabe: agil oder klassisch. Es gibt aber noch einen anderen Ansatz, mit diesen "unterschiedlichen Welten" umzugehen: Und zwar die beiden Philosophien innerhalb eines Projekts zu kombinieren. Wie dies in der Praxis aussehen und gelingen kann, zeigen Dr. Michael Kirchhof und Prof. Dr. Bodo Kraft in diesem Beitrag. Y1 - 2012 IS - 11 SP - 11 S. PB - Berleb Media CY - Taufkirchen ER - TY - JOUR A1 - Grotendorst, Johannes T1 - IAS Winter School: Hierarchical Methods for Dynamics in Complex Molecular Systems JF - Innovatives Supercomputing in Deutschland : inSiDE. 10 (2012), H. 1 Y1 - 2012 SP - 104 PB - - ER - TY - JOUR A1 - Lempiäinen, Harri A1 - Couttet, Philippe A1 - Bolognani, Federico A1 - Müller, Arne A1 - Dubost, Valérie A1 - Luisier, Raphaëlle A1 - Rio-Espinola, Alberto del A1 - Vitry, Veronique A1 - Unterberger, Elif B. A1 - Thomson, John P. A1 - Treindl, Fridolin A1 - Metzger, Ute A1 - Wrzodek, Clemens A1 - Hahne, Florian A1 - Zollinger, Tulipan A1 - Brasa, Sarah A1 - Kalteis, Magdalena A1 - Marcellin, Magali A1 - Giudicelli, Fanny A1 - Braeuning, Albert A1 - Morawiec, Laurent A1 - Zamurovic, Natasa A1 - Längle, Ulrich A1 - Scheer, Nico A1 - Schübeler, Dirk A1 - Goodman, Jay A1 - Chibout, Salah-Dine A1 - Marlowe, Jennifer A1 - Theil, Dietlinde A1 - Heard, David J. A1 - Grenet, Olivier A1 - Zell, Andreas A1 - Templin, Markus F. A1 - Meehan, Richard R. A1 - Wolf, Roland C. A1 - Elcombe, Clifford R. A1 - Schwarz, Michael A1 - Moulin, Pierre A1 - Terranova, Rémi A1 - Moggs, Jonathan G. T1 - Identification of Dlk1-Dio3 imprinted gene cluster non-coding RNAs as novel candidate biomarkers for liver tumor promotion JF - Toxicological Sciences N2 - The molecular events during nongenotoxic carcinogenesis and their temporal order are poorly understood but thought to include long-lasting perturbations of gene expression. Here, we have investigated the temporal sequence of molecular and pathological perturbations at early stages of phenobarbital (PB) mediated liver tumor promotion in vivo. Molecular profiling (mRNA, microRNA [miRNA], DNA methylation, and proteins) of mouse liver during 13 weeks of PB treatment revealed progressive increases in hepatic expression of long noncoding RNAs and miRNAs originating from the Dlk1-Dio3 imprinted gene cluster, a locus that has recently been associated with stem cell pluripotency in mice and various neoplasms in humans. PB induction of the Dlk1-Dio3 cluster noncoding RNA (ncRNA) Meg3 was localized to glutamine synthetase-positive hypertrophic perivenous hepatocytes, sug- gesting a role for β-catenin signaling in the dysregulation of Dlk1-Dio3 ncRNAs. The carcinogenic relevance of Dlk1-Dio3 locus ncRNA induction was further supported by in vivo genetic dependence on constitutive androstane receptor and β-catenin pathways. Our data identify Dlk1-Dio3 ncRNAs as novel candidate early biomarkers for mouse liver tumor promotion and provide new opportunities for assessing the carcinogenic potential of novel compounds. Y1 - 2012 U6 - http://dx.doi.org/10.1093/toxsci/kfs303 SN - 1094-2025 VL - 131 IS - 2 SP - 375 EP - 386 PB - Oxford University Press CY - Oxford ER - TY - JOUR A1 - Frings, Michael T1 - Immobilienfinanzierung und Verbraucherkredit : Das neue Verbraucherkreditrecht nach der Verbraucherkreditrichtlinie JF - NWB : Steuer- und Wirtschaftsrecht N2 - Mit Gesetz vom 29.7.2009 (BGBl 2009 I S. 2355) hat der deutsche Gesetzgeber die EU-Verbraucherkreditrichtlinie 2008/48/EG vom 23.4.2008 NWB PAAAD-28762 in nationales Recht umgesetzt. In diesem Gesetz ist auch der Immobiliardarlehensvertrag neu geregelt worden. Auf diese besondere Kreditform sind grds. die Vorschriften zum Verbraucherkredit anzuwenden, so dass der Darlehensgeber auch im Fall einer Immobilienfinanzierung zu vorvertraglicher Information des Verbrauchers verpflichtet ist. Beim finanzierten Immobilienerwerb werden Veräußerer und kreditgebende Bank häufig in Abstimmung und Zusammenarbeit tätig, so dass für die Bank besondere Aufklärungspflichten gegenüber dem Verbraucher entstehen. Im Fall der Verletzung dieser Pflichten drohen ihr Schadensersatzansprüche oder ein Widerrufsrecht des Verbrauchers. Y1 - 2012 SN - 0028-3460 VL - 2012 IS - 15 SP - 1237 EP - 1247 PB - NWB-Verlag CY - Herne ER - TY - JOUR A1 - Mottaghy, Darius A1 - Dijkshoorn, Lydia T1 - Implementing an effective finite difference formulation for borehole heat exchangers into a heat and mass transport code JF - Renewable Energy N2 - We present an effective finite difference formulation for implementing and modeling multiple borehole heat exchangers (BHE) in the general 3-D coupled heat and flow transport code SHEMAT. The BHE with arbitrary length can be either coaxial or double U-shaped. It is particularly suitable for modeling deep BHEs which contain varying pipe diameters and materials. Usually, in numerical simulations, a fine discretization of the BHE assemblage is required, due to the large geometric aspect ratios involved. This yields large models and long simulation times. The approach avoids this problem by considering heat transport between fluid and the soil through pipes and grout via thermal resistances. Therefore, the simulation time can be significantly reduced. The coupling with SHEMAT is realized by introducing an effective heat generation. Due to this connection, it is possible to consider heterogeneous geological models, as well as the influence of groundwater flow. This is particularly interesting when studying the long term behavior of a single BHE or a BHE field. Heating and cooling loads can enter the model with an arbitrary interval, e.g. from hourly to monthly values. When dealing with large BHE fields, computing times can be further significantly reduced by focusing on the temperature field around the BHEs, without explicitly modeling inlet and outlet temperatures. This allows to determine the possible migration of cold and warm plumes due to groundwater flow, which is of particular importance in urban areas with a high BHE installation density. The model is validated against the existing BHE modeling codes EWS and EED. A comparison with monitoring data from a deep BHE in Switzerland shows a good agreement. Synthetic examples demonstrate the field of application of this model. KW - Borehole heat exchanger KW - Finite differences KW - Heat transport Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.renene.2012.02.013 SN - 0960-1481 VL - 45 SP - 59 EP - 71 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Kobus, Thiele A1 - Bitz, Andreas A1 - Uden, Mark J. van A1 - Lagemaat, Miram W. A1 - Rothgang, Eva A1 - Orzada, Stephan A1 - Heerschap, Arend A1 - Scheenen, Tom W. J. T1 - In vivo 31P MR spectroscopic imaging of the human prostate at 7 T: safety and feasibility JF - Magnetic Resonance in Medicine N2 - 31P MR spectroscopic imaging of the human prostate provides information about phosphorylated metabolites that could be used for prostate cancer characterization. The sensitivity of a magnetic field strength of 7 T might enable 3D 31P MR spectroscopic imaging with relevant spatial resolution in a clinically acceptable measurement time. To this end, a 31P endorectal coil was developed and combined with an eight-channel 1H body-array coil to relate metabolic information to anatomical location. An extensive safety validation was performed to evaluate the specific absorption rate, the radiofrequency field distribution, and the temperature distribution of both coils. This validation consisted of detailed Finite Integration Technique simulations, confirmed by MR thermometry and Burn:x-wiley:07403194:media:MRM24175:tex2gif-stack-1 measurements in a phantom and in vivo temperature measurements. The safety studies demonstrated that the presence of the 31P endorectal coil had no influence on the specific absorption rate levels and temperature distribution of the external eight-channel 1H array coil. To stay within a 10 g averaged local specific absorption rate of 10 W/kg, a maximum time-averaged input power of 33 W for the 1H array coil was allowed. For transmitting with the 31P endorectal coil, our safety limit of less than 1°C temperature increase in vivo during a 15-min MR spectroscopic imaging experiment was reached at a time-averaged input power of 1.9 W. With this power setting, a second in vivo measurement was performed on a healthy volunteer. Using adiabatic excitation, 3D 31P MR spectroscopic imaging produced spectra from the entire prostate in 18 min with a spatial resolution of 4 cm3. The spectral resolution enabled the separate detection of phosphocholine, phosphoethanolamine, inorganic phosphate, and other metabolites that could play an important role in the characterization of prostate cancer. Y1 - 2012 U6 - http://dx.doi.org/10.1002/mrm.24175 SN - 1522-2594 VL - 68 IS - 6 SP - 1683 EP - 1695 PB - Wiley-Liss CY - New York ER - TY - JOUR A1 - Nguyen, Nhu Huynh A1 - Duong, Minh Tuan A1 - Tran, Thanh Ngoc A1 - Pham, Phu Tinh A1 - Grottke, O. A1 - Tolba, R. A1 - Staat, Manfred T1 - Influence of a freeze–thaw cycle on the stress–stretch curves of tissues of porcine abdominal organs JF - Journal of Biomechanics N2 - The paper investigates both fresh porcine spleen and liver and the possible decomposition of these organs under a freeze–thaw cycle. The effect of tissue preservation condition is an important factor which should be taken into account for protracted biomechanical tests. In this work, tension tests were conducted for a large number of tissue specimens from twenty pigs divided into two groups of 10. Concretely, the first group was tested in fresh state; the other one was tested after a freeze-thaw cycle which simulates the conservation conditions before biomechanical experiments. A modified Fung model for isotropic behavior was adopted for the curve fitting of each kind of tissues. Experimental results show strong effects of the realistic freeze–thaw cycle on the capsule of elastin-rich spleen but negligible effects on the liver which virtually contains no elastin. This different behavior could be explained by the autolysis of elastin by elastolytic enzymes during the warmer period after thawing. Realistic biomechanical properties of elastin-rich organs can only be expected if really fresh tissue is tested. The observations are supported by tests of intestines. KW - Autolysis KW - Decomposition KW - Freeze–thaw process KW - Spleen KW - Liver Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.jbiomech.2012.07.008 SN - 1873-2380 VL - 45 IS - 14 SP - 2382 EP - 2386 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Oertel, Mario A1 - Bung, Daniel B. T1 - Initial stage of two-dimensional dam-break waves: laboratory versus VOF JF - Journal of hydraulic research N2 - Since several decades, dam-break waves have been of main research interest. Mathematical approaches have been developed by analytical, physical and numerical models within the past 120 years. During the past 10 years, the number of research investigations has increased due to improved measurement techniques as well as significantly increased computer memories and performances. In this context, the present research deals with the initial stage of two-dimensional dam-break waves by comparing physical and numerical model results as well as analytical approaches. High-speed images and resulting particle image velocimetry calculations are thereby compared with the numerical volume-of-fluid (VOF) method, included in the commercial code FLOW-3D. Wave profiles and drag forces on placed obstacles are analysed in detail. Generally, a good agreement between the laboratory and VOF results is found. KW - VOF KW - PIV KW - physical model KW - numerical model KW - drag force KW - dam-break Y1 - 2012 U6 - http://dx.doi.org/10.1080/00221686.2011.639981 SN - 1814-2079 (E-Journal); 0022-1686 (Print) VL - 50 IS - 1 SP - 89 EP - 97 PB - Taylor & Francis CY - London ER - TY - JOUR A1 - Butenweg, Christoph A1 - Gellert, Christoph T1 - Integrale Gebäudeplanung am Beispiel eines Geschossbaus in Ziegelmauerwerk JF - Mauerwerk : European journal of masonry N2 - Moderne Mauerwerksbauten müssen nach heutigen Anforderungen architektonisch, statisch, energetisch sowie schall- und brandschutztechnisch optimal ausgelegt sein. Aufgrund der Komplexität und engen Verzahnung der einzelnen Anforderungen ist eine integrale Gebäudeplanung zur Erzielung einer qualitativ hochwertigen Bauwerkslösung unbedingt notwendig. Diese erfordert von den Fachplanern vertieftes Spezialwissen in den verschiedenen Bereichen, um insbesondere die Schnittstellen und Bauwerksdetails korrekt zu planen und auszuführen. Der Beitrag stellt die integrale Gebäudeplanung am Beispiel eines Geschossbaus in Ziegelbauweise mit Lösungen für wesentliche Detailpunkte vor Y1 - 2012 U6 - http://dx.doi.org/10.1002/dama.201200550 SN - 1437-1022 (E-Journal); 1432-3427 (Print) VL - Volume 16 IS - 5 SP - 247 EP - 254 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Loeb, Horst Wolfgang A1 - Schartner, Karl-Heinz A1 - Dachwald, Bernd A1 - Ohndorf, Andreas A1 - Seboldt, Wolfgang T1 - Interstellar heliopause probe JF - Труды МАИ N2 - There is common agreement within the scientific community that in order to understand our local galactic environment it will be necessary to send a spacecraft into the region beyond the solar wind termination shock. Considering distances of 200 AU for a new mission, one needs a spacecraft traveling at a speed of close to 10 AU/yr in order to keep the mission duration in the range of less than 25 yrs, a transfer time postulated by European Space Agency (ESA). Two propulsion options for the mission have been proposed and discussed so far: the solar sail propulsion and the ballistic/radioisotope-electric propulsion (REP). As a further alternative, we here investigate a combination of solar-electric propulsion (SEP) and REP. The SEP stage consists of six 22-cms diameter RIT-22 ion thrusters working with a high specific impulse of 7377 s corresponding to a positive grid voltage of 5 kV. Solar power of 53 kW at begin of mission (BOM) is provided by a lightweight solar array. Y1 - 2012 IS - 60 SP - 2 EP - 2 PB - Moskauer Staatliches Luftfahrtinstitut (МАИ) CY - Moskau ER - TY - JOUR A1 - Reisgen, Uwe A1 - Schleser, Markus A1 - Abdurakhmanov, Aydemir A1 - Turichin, Gleb A1 - Valdaitseva, Elena A1 - Bach, Friedrich-Wilhelm A1 - Hassel, Thomas A1 - Beniyashi, Alexander T1 - Investigation of factors influencing the formation of weld defects in non-vacuum electron beam welding JF - The Paton welding journal N2 - The influence of welding condition parameters and properties of material on formation of defects, such as humping and undercuts, in non-vacuum electron beam welding was investigated. The influence of separate welding parameters on the quality of welds was determined. Y1 - 2012 SN - 0957-798X VL - 2012 IS - 2 SP - 11 EP - 18 PB - Paton Publishing House CY - Kiev ER - TY - JOUR A1 - Schneider, Bettina A1 - Schneider, Wilhelm T1 - Jahresabschluss nach BilMoG : Ansatz-, Ausweis- und Bewertungsvorschriften JF - Das Wirtschaftsstudium : wisu ; Zeitschrift für Ausbildung, Examen, Berufseinstieg und Fortbildung Y1 - 2012 SN - 0340-3084 VL - 41 IS - 4 SP - 529 EP - 535 PB - Lange CY - Düsseldorf ER - TY - JOUR A1 - Backes, Klaus A1 - Adam, Mario A1 - Gottschald, Beng Jonas A1 - Faber, Christian A1 - Henneböhl, Friedrich-Gregor A1 - Lanz, Marco A1 - Anthrakidis, Anette T1 - KWKK-Anlagen im Feldtest. Optimaler Betrieb durch Verbesserungen bei Regelung und Hydraulik JF - Die Kälte- und Klimatechnik Y1 - 2012 SN - 0343-2246 IS - 2 PB - Gentner CY - Stuttgart ER - TY - JOUR A1 - Abouzar, Maryam H. A1 - Poghossian, Arshak A1 - Cherstvy, Andrey G. A1 - Pedraza, Angela M. A1 - Ingebrandt, Sven A1 - Schöning, Michael Josef T1 - Label-free electrical detection of DNA by means of field-effect nanoplate capacitors: Experiments and modeling JF - Physica Status Solidi (a) N2 - Label-free electrical detection of consecutive deoxyribonucleic acid (DNA) hybridization/denaturation by means of an array of individually addressable field-effect-based nanoplate silicon-on-insulator (SOI) capacitors modified with gold nanoparticles (Au-NP) is investigated. The proposed device detects charge changes on Au-NP/DNA hybrids induced by the hybridization or denaturation event. DNA hybridization was performed in a high ionic-strength solution to provide a high hybridization efficiency. On the other hand, to reduce the screening of the DNA charge by counter ions and to achieve a high sensitivity, the sensor signal induced by the hybridization and denaturation events was measured in a low ionic-strength solution. High sensor signals of about 120, 90, and 80 mV were registered after the DNA hybridization, denaturation, and re-hybridization events, respectively. Fluorescence microscopy has been applied as reference method to verify the DNA immobilization, hybridization, and denaturation processes. An electrostatic charge-plane model for potential changes at the gate surface of a nanoplate field-effect sensor induced by the DNA hybridization has been developed taking into account both the Debye length and the distance of the DNA charge from the gate surface. Y1 - 2012 U6 - http://dx.doi.org/10.1002/pssa.201100710 SN - 1862-6319 VL - 209 SP - 925 EP - 934 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Reisgen, Uwe A1 - Olschok, Simon A1 - Jakobs, Stefan A1 - Schleser, Markus A1 - Mokrov, Oleg A1 - Rossiter, Eduardo T1 - Laser beam submerged arc hybrid welding JF - Physics procedia N2 - The laser beam-submerged arc hybrid welding method originates from the knowledge that, with increasing penetration depth, the laser beam process has a tendency to pore formation in the lower weld regions. The coupling with the energy-efficient submerged-arc process improves degassing and reduces the tendency to pore formation. The high deposition rate of the SA process in combination with the laser beam process offers, providing the appropriate choice of weld preparation, the possibility of welding plates with a thickness larger than 20° mm in a single pass, and also of welding thicker plates with the double-sided single pass technique. Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.phpro.2012.10.016 SN - 1875-3892 N1 - Part of special issue "Laser assisted net shape engineering 7 (LANE 2012)" VL - 39 SP - 75 EP - 83 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Wagner, Torsten A1 - Shigiahara, N. A1 - Miyamoto, K. A1 - Suzurikawa, J. A1 - Finger, F. A1 - Schöning, Michael Josef A1 - Yoshinobu, T. T1 - Light-addressable Potentiometric Sensors and Light–addressable Electrodes as a Combined Sensor-and-manipulator Microsystem with High Flexibility JF - Procedia Engineering N2 - This work describes the novel combination of the light-addressable electrode (LAE) and the light-addressable potentiometric sensor (LAPS) into a microsystem set-up. Both the LAE as well as the LAPS shares the principle of addressing the active spot by means of a light beam. This enables both systems to manipulate resp. to detect an analyte with a high spatial resolution. Hence, combining both principles into a single set-up enables the active stimulation e.g., by means of electrolysis and a simultaneous observation e.g., the response of an entrapped biological cell by detection of extracellular pH changes. The work will describe the principles of both technologies and the necessary steps to integrate them into a single set-up. Furthermore, examples of application and operation of such systems will be presented. Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.proeng.2012.09.290 SN - 1877-7058 N1 - Part of special issue "26th European Conference on Solid-State Transducers, EUROSENSOR 2012" IS - 47 SP - 890 EP - 893 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Staat, Manfred A1 - Vu, Duc Khoi T1 - Limit analysis of flaws in pressurized pipes and cylindrical vessels Part II: Circumferential defects JF - Engineering Fracture Mechanics ; 97(2013), H. 1 N2 - Upper and lower bound theorems of limit analyses have been presented in part I of the paper. Part II starts with the finite element discretization of these theorems and demonstrates how both can be combined in a primal–dual optimization problem. This recently proposed numerical method is used to guide the development of a new class of closed-form limit loads for circumferential defects, which show that only large defects contribute to plastic collapse with a rapid loss of strength with increasing crack sizes. The formulae are compared with primal–dual FEM limit analyses and with burst tests. Even closer predictions are obtained with iterative limit load solutions for the von Mises yield function and for the Tresca yield function. Pressure loading of the faces of interior cracks in thick pipes reduces the collapse load of circumferential defects more than for axial flaws. Axial defects have been treated in part I of the paper. Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.engfracmech.2012.05.017 SN - 0013-7944 VL - 97 SP - 314 EP - 333 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Staat, Manfred T1 - Limit and shakedown analysis under uncertainty JF - Tap chi Khoa hoc & ung dung - Dai hoc Ton Duc Thang Y1 - 2012 N1 - = Journal of Applied Sciences - Ton Duc Thang University VL - 19 SP - 45 EP - 47 ER - TY - JOUR A1 - Reisgen, Uwe A1 - Schleser, Markus A1 - Abdurakhmanov, Aydemir A1 - Gumenyuk, Andrey T1 - Measuring of plasma properties induced by non-vacuum electron beam welding JF - Physics of plasma N2 - Electron beam plasma measurement was realised by means of DIABEAM system invented by ISF RWTH Aachen. The Langmuir probe method is used for measurement. The relative simplicity of the method and the possibility of dispersion of high power on the probe allow its application for the investigation of high-power electron beams. The key element of the method is a rotating thin tungsten wire, which intersects the beam transversely on its axis and collects part of the current by itself. The signals, which are registered in the DIABEAM as a voltage, were taken in the form of amplitude. The conversion of the probe current into the distribution along the beam radius was realised using the Abel’s method. A voltage-current characteristic was built for the beam current. The local electron density as well as the electron temperature, the floating potential and the plasma potential were measured and calculated by means of this characteristic. Y1 - 2012 U6 - http://dx.doi.org/10.1063/1.3675874 SN - 1089-7674 (E-Journal); 1070-664X (Print) VL - 19 IS - 1 SP - 1 EP - 7 PB - AIP Publishing CY - Melville, NY ER - TY - JOUR A1 - Lustfeld, H. A1 - Pithan, C. A1 - Reißel, Martin T1 - Metallic electrolyte composites in the framework of the brick-layer model JF - Journal of the European Ceramic Society N2 - It is well known that the already large dielectric constants of some electrolytes like BaTiO₃ can be enhanced further by adding metallic (e.g. Ni, Cu or Ag) nanoparticles. The enhancement can be quite large, a factor of more than 1000 is possible. The consequences for the properties will be discussed in the present paper applying a brick-layer model (BLM) for calculating dc-resistivities of thin layers and a modified one (PBLM) that includes percolation for calculating dielectric properties of these materials. The PBLM results in an at least qualitative description and understanding of the physical phenomena: This model gives an explanation for the steep increase of the dielectric constant below the percolation threshold and why this increase is connected to a dramatic decrease of the breakdown voltage as well as the ability of storing electrical energy. We conclude that metallic electrolyte composites like BaTiO₃ are not appropriate for energy storage. Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.jeurceramsoc.2011.10.017 SN - 0955-2219 VL - 32 IS - 4 SP - 859 EP - 864 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Bäcker, Matthias A1 - Raue, Markus A1 - Schusser, Sebastian A1 - Jeitner, C. A1 - Breuer, L. A1 - Wagner, P. A1 - Poghossian, Arshak A1 - Förster, Arnold A1 - Mang, Thomas A1 - Schöning, Michael Josef T1 - Microfluidic chip with integrated microvalves based on temperature- and pH-responsive hydrogel thin films JF - Physica Status Solidi (a) N2 - Two types of microvalves based on temperature-responsive poly(N-isopropylacrylamide) (PNIPAAm) and pH-responsive poly(sodium acrylate) (PSA) hydrogel films have been developed and tested. The PNIPAAm and PSA hydrogel films were prepared by means of in situ photopolymerization directly inside the fluidic channel of a microfluidic chip fabricated by combining Si and SU-8 technologies. The swelling/shrinking properties and height changes of the PNIPAAm and PSA films inside the fluidic channel were studied at temperatures of deionized water from 14 to 36 °C and different pH values (pH 3–12) of Titrisol buffer, respectively. Additionally, in separate experiments, the lower critical solution temperature (LCST) of the PNIPAAm hydrogel was investigated by means of a differential scanning calorimetry (DSC) and a surface plasmon resonance (SPR) method. Mass-flow measurements have shown the feasibility of the prepared hydrogel films to work as an on-chip integrated temperature- or pH-responsive microvalve capable to switch the flow channel on/off. Y1 - 2012 U6 - http://dx.doi.org/10.1002/pssa.201100763 SN - 1862-6319 VL - 209 IS - 5 SP - 839 EP - 845 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Miyamoto, Ko-ichiro A1 - Kaneko, Kazumi A1 - Matsuo, Akira A1 - Wagner, Torsten A1 - Kanoh, Shiníchiro A1 - Schöning, Michael Josef A1 - Yoshinobu, Tatsuo T1 - Miniaturized chemical imaging sensor system using an OLED display panel JF - Sensors and Actuators B: Chemical N2 - The chemical imaging sensor is a semiconductor-based chemical sensor that can visualize the two-dimensional distribution of specific ions or molecules in the solution. In this study, we developed a miniaturized chemical imaging sensor system with an OLED display panel as a light source that scans the sensor plate. In the proposed configuration, the display panel is placed directly below the sensor plate and illuminates the back surface. The measured area defined by illumination can be arbitrarily customized to fit the size and the shape of the sample to be measured. The waveform of the generated photocurrent, the current–voltage characteristics and the pH sensitivity were investigated and pH imaging with this miniaturized system was demonstrated. KW - LAPS KW - Light-addressable potentiometric sensor KW - Chemical imaging sensor KW - Organic light-emitting diode display Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.snb.2011.02.029 SN - 0925-4005 N1 - Part of special issue "Eurosensors XXIV, 2010" VL - 170 SP - 82 EP - 87 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Verbanella, Stresa A1 - Fabo, Sabine T1 - Mit Pinsel und Farbe hacken : Interview mit Sabine Fabo T1 - Hacking with paint and brush : interview with Sabine Fabo JF - Remotewords.net N2 - Remotewords makes its mark on the picturesque "Bar Verbanella”, located in Stresa near Isola Bella on Lago Maggiore. The words CUM GRANO SALIS (with a grain of salt) by Sabine Fabo, art theorist on media contexts, were applied to its roof in collaboration with the German-Italian art organization "Lo spirito del Lago". KW - Guerrillawerbung KW - Parasitäre Strategie Y1 - 2012 ER - TY - JOUR A1 - Scheer, Nico A1 - Kapelyukh, Yury A1 - McEwan, Jillian A1 - Beuger, Vincent A1 - Stanley, Lesley A. A1 - Rode, Anja A1 - Wolf, C. Roland T1 - Modeling Human Cytochrome P450 2D6 Metabolism and Drug-drug Interaction by a Novel Panel of Knockout and Humanized Mouse Lines JF - Molecular Pharmacology N2 - The highly polymorphic human cytochrome P450 2D6 enzyme is involved in the metabolism of up to 25% of all marketed drugs and accounts for significant individual differences in response to CYP2D6 substrates. Because of the differences in the multiplicity and substrate specificity of CYP2D family members among species, it is difficult to predict pathways of human CYP2D6-dependent drug metabolism on the basis of animal studies. To create animal models that reflect the human situation more closely and that allow an in vivo assessment of the consequences of differential CYP2D6 drug metabolism, we have developed a novel straightforward approach to delete the entire murine Cyp2d gene cluster and replace it with allelic variants of human CYP2D6. By using this approach, we have generated mouse lines expressing the two frequent human protein isoforms CYP2D6.1 and CYP2D6.2 and an as yet undescribed variant of this enzyme, as well as a Cyp2d cluster knockout mouse. We demonstrate that the various transgenic mouse lines cover a wide spectrum of different human CYP2D6 metabolizer phenotypes. The novel humanization strategy described here provides a robust approach for the expression of different CYP2D6 allelic variants in transgenic mice and thus can help to evaluate potential CYP2D6-dependent interindividual differences in drug response in the context of personalized medicine. Y1 - 2012 U6 - http://dx.doi.org/10.1124/mol.111.075192 SN - 1521-0111 VL - 81 IS - 1 SP - 63 EP - 72 PB - ASPET CY - Bethesda, Md. ER - TY - JOUR A1 - Reisgen, Uwe A1 - Schleser, Markus A1 - Mokrov, Oleg A1 - Zabirov, Alexander A1 - Füssel, Uwe A1 - Schnick, Michael A1 - Hertel, Martin A1 - Jaeckel, Sebastian T1 - Modellierung und Visualisierung der MSG-Lichtbogenprozesse JF - Schweissen und Schneiden : Fachzeitschrift für Schweißen und verwandte Verfahren Y1 - 2012 SN - 0036-7184 VL - 64 IS - 4 SP - 166 EP - 174 PB - DVS Verlag CY - Düsseldorf ER - TY - JOUR A1 - Reisgen, Uwe A1 - Schleser, Markus A1 - Mokrov, Oleg A1 - Zabirov, Alexander A1 - Füssel, Uwe A1 - Schnick, Michael A1 - Hertel, Martin A1 - Jaeckel, Sebastian T1 - Modelling and visualisation of the GMA process JF - Welding and Cutting Y1 - 2012 SN - 1612-3433 VL - 11 IS - 4 SP - 242 EP - 249 PB - DVS Verlag CY - Düsseldorf ER - TY - JOUR A1 - Bohrn, U. A1 - Stütz, E. A1 - Fuchs, K. A1 - Fleischer, M. A1 - Schöning, Michael Josef A1 - Wagner, P. T1 - Monitoring of irritant gas using a whole-cell-based sensor system JF - Sensors and Actuators B: Chemical N2 - Cell-based sensors for the detection of gases have long been underrepresented, due to the cellular requirement of being cultured in a liquid environment. In this work we established a cell-based gas biosensor for the detection of toxic substances in air, by adapting a commercial sensor chip (Bionas®), previously used for the measurement of pollutants in liquids. Cells of the respiratory tract (A549, RPMI 2650, V79), which survive at a gas phase in a natural context, are used as biological receptors. The physiological cell parameters acidification, respiration and morphology are continuously monitored in parallel. Ammonia was used as a highly water-soluble model gas to test the feasibility of the sensor system. Infrared measurements confirmed the sufficiency of the medium draining method. This sensor system provides a basis for many sensor applications such as environmental monitoring, building technology and public security. Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.snb.2012.05.088 SN - 0925-4005 VL - 175 SP - 208 EP - 217 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Butenweg, Christoph A1 - Holtschoppen, Britta T1 - Neuer VCI-Leitfaden für die erdbebensichere Auslegung von Industrieanlagen JF - Bauingenieur : die richtungsweisende Zeitschrift im Bauingenieurswesen ; offizielle Zeitschrift der VDI-Gesellschaft Bautechnik Y1 - 2012 SN - 1436-4867 (E-Journal); 0005-6650 (Print) VL - Band 87 SP - S18 PB - VDI Fachmedien CY - Düsseldorf ER - TY - JOUR A1 - Staat, Manfred A1 - Trenz, Eva A1 - Lohmann, Philipp A1 - Frotscher, Ralf A1 - Klinge, Uwe A1 - Tabaza, Ruth A1 - Kirschner-Hermanns, Ruth T1 - New measurements to compare soft tissue anchoring systems in pelvic floor surgery JF - Journal of Biomedical Materials Research Part B: Applied Biomaterials N2 - Suburethral slings as well as different meshes are widely used treating stress urinary incontinence and prolaps in women. With the development of MiniSlings and special meshes using less alloplastic material anchorage systems become more important to keep devices in place and to put some tension especially on the MiniSlings. To date, there are many different systems of MiniSlings of different companies on the market which differ in the structure of the used meshes and anchors. A new objective measurement method to compare different properties of MiniSling systems (mesh and anchor) is presented in this article. Ballistic gelatine acts as soft tissue surrogate. Significant differences in parameters like pull-out strength of anchors or shrinkage of meshes under loading conditions have been determined. The form and size of the anchors as well as the structural stability of the meshes are decisive for a proper integration. The tested anchorings sytems showed markedly different mechanical function at their respective load bearing capacity. As the stable fixation of the device in tissue is a prerequisite for a permanet reinforcement, the proposed test system permits further optimisation of anchor and mesh devices to improve the success of the surgical treatment Y1 - 2012 U6 - http://dx.doi.org/10.1002/jbm.b.32654 SN - 1552-4981 VL - 100B IS - 4 SP - 924 EP - 933 PB - Wiley CY - Hoboken, NJ ER - TY - JOUR A1 - Iken, Heiko A1 - Kirsanov, D. A1 - Legin, A. A1 - Schöning, Michael Josef T1 - Novel Thin-Film Polymeric Materials for the Detection of Heavy Metals JF - Procedia Engineering N2 - A variety of transition metals, e.g., copper, zinc, cadmium, lead, etc. are widely used in industry as components for wires, coatings, alloys, batteries, paints and so on. The inevitable presence of transition metals in industrial processes implies the ambition of developing a proper analytical technique for their adequate monitoring. Most of these elements, especially lead and cadmium, are acutely toxic for biological organisms. Quantitative determination of these metals at low activity levels in different environmental and industrial samples is therefore a vital task. A promising approach to achieve an at-side or on-line monitoring on a miniaturized and cost efficient way is the combination of a common potentiometric sensor array with heavy metal-sensitive thin-film materials, like chalcogenide glasses and polymeric materials, respectively. Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.proeng.2012.09.148 SN - 1877-7058 N1 - Part of special issue "26th European Conference on Solid-State Transducers, EUROSENSOR 2012" IS - 47 SP - 322 EP - 325 PB - Elsevier CY - Amsterdam ER -