TY  - JOUR
A1  - Jablonski, Melanie
A1  - Münstermann, Felix
A1  - Nork, Jasmina
A1  - Molinnus, Denise
A1  - Muschallik, Lukas
A1  - Bongaerts, Johannes
A1  - Wagner, Torsten
A1  - Keusgen, Michael
A1  - Siegert, Petra
A1  - Schöning, Michael Josef
T1  - Capacitive field‐effect biosensor applied for the detection of acetoin in alcoholic beverages and fermentation broths
JF  - physica status solidi (a) applications and materials science
N2  - An acetoin biosensor based on a capacitive electrolyte–insulator–semiconductor (EIS) structure modified with the enzyme acetoin reductase, also known as butane-2,3-diol dehydrogenase (Bacillus clausii DSM 8716ᵀ), is applied for acetoin detection in beer, red wine, and fermentation broth samples for the first time. The EIS sensor consists of an Al/p-Si/SiO₂/Ta₂O₅ layer structure with immobilized acetoin reductase on top of the Ta₂O₅ transducer layer by means of crosslinking via glutaraldehyde. The unmodified and enzyme-modified sensors are electrochemically characterized by means of leakage current, capacitance–voltage, and constant capacitance methods, respectively.
KW  - acetoin
KW  - acetoin reductase
KW  - alcoholic beverages
KW  - biosensors
KW  - capacitive field-effect sensors
Y1  - 2021
U6  - http://dx.doi.org/10.1002/pssa.202000765
SN  - 1862-6319
VL  - 218
IS  - 13
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - PAT
A1  - Lauth, Jakob
A1  - Hagemeyer, Alfred
A1  - Lautensack, Thomas
A1  - Deimling, Axel
T1  - Catalyst and catalytic oxidative dehydrogenation of alkylaromatics and paraffins : United States Patent ; patent number 5,902,918 ; date of patent: May 11, 1999 / Assignee: BASF Aktiengesellschaft. Inventors: Alfred Hagemeyer ; Gunter Lauth ; Thomas Lautensack ; Axel Deimling
Y1  - 1999
N1  - Barcode: US005902918A ; Volltext über Datenbank: http://www.google.com/patents ; Falsche Namensansetzung: Gunter ...
PB  - United States Trademark and Patent Office
CY  - [Alexandria, VA]
ER  - 
TY  - CHAP
A1  - Wendorff, Marion
A1  - Eggert, Thorsten
A1  - Pohl, Martina
A1  - Dresen, Carola
A1  - Müller, Michael
A1  - Jaeger, Karl-Erich
A1  - Sprenger, Georg A.
A1  - Schürmann, Melanie
A1  - Schürmann, Martin
A1  - Johnen, Sandra
A1  - Sprenger, Gerda
A1  - Sahm, Hermann
A1  - Inoue, Tomoyuki
A1  - Schörken, Ulrich
A1  - Breittaupt, Holger
A1  - Frölich, Bettina
A1  - Heim, Petra
A1  - Iding, Hans
A1  - Juchem, Bettina
A1  - Siegert, Petra
A1  - Kula, Maria-Regina
A1  - Weckbecker, Andrea
A1  - Hummel, Werner
A1  - Fessner, Wolf-Dieter
A1  - Elling, Lothar
A1  - Wolberg, Michael
A1  - Bode, Silke
A1  - Feldmann, Ralf
A1  - Geilenkirchen, Petra
A1  - Schubert, Thomas
A1  - Walter, Lydia
A1  - Dünnwald, Thomas
A1  - Demir, Ayhan S.
A1  - Kolter-Jung, Doris
A1  - Nitsche, Adam
A1  - Dünkelmann, Pascal
A1  - Cosp, Annabel
A1  - Lingen, Bettina
T1  - Catalytic asymmetric synthesis : section 2.2
T2  - Asymmetric synthesis with chemical and biological methods / ed. by Dieter Enders ...
Y1  - 2007
SN  - 978-3-527-31473-7
SP  - 298
EP  - 413
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Handtke, Stefan
A1  - Volland, Sonja
A1  - Methling, Karen
A1  - Albrecht, Dirk
A1  - Becher, Dörte
A1  - Nehls, Jenny
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - Lalk, Michael
A1  - Liesegang, Heiko
A1  - Voigt, Birgit
A1  - Daniel, Rolf
A1  - Hecker, Michael
T1  - Cell physiology of the biotechnological relevant bacterium Bacillus pumilus - An omics-based approach
JF  - Journal of Biotechnology
N2  - Members of the species Bacillus pumilus get more and more in focus of the biotechnological industry as potential new production strains. Based on exoproteome analysis, B. pumilus strain Jo2, possessing a high secretion capability, was chosen for an omics-based investigation. The proteome and metabolome of B. pumilus cells growing either in minimal or complex medium was analyzed. In total, 1542 proteins were identified in growing B. pumilus cells, among them 1182 cytosolic proteins, 297 membrane and lipoproteins and 63 secreted proteins. This accounts for about 43% of the 3616 proteins encoded in the B. pumilus Jo2 genome sequence. By using GC–MS, IP-LC/MS and H NMR methods numerous metabolites were analyzed and assigned to reconstructed metabolic pathways. In the genome sequence a functional secretion system including the components of the Sec- and Tat-secretion machinery was found. Analysis of the exoproteome revealed secretion of about 70 proteins with predicted secretion signals. In addition, selected production-relevant genome features such as restriction modification systems and NRPS clusters of B. pumilus Jo2 are discussed.
Y1  - 2014
U6  - http://dx.doi.org/10.1016/j.jbiotec.2014.08.028
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
IS  - 192(A)
SP  - 204
EP  - 214
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Koch, Linda
A1  - Wunderlich, F. Thomas
A1  - Seibler, Jost
A1  - Könner, A. Christine
A1  - Hampel, Brigitte
A1  - Irlenbusch, Sigrid
A1  - Brabant, Georg
A1  - Kahn, C. Ronald
A1  - Schwenk, Frieder
A1  - Brüning, Jens C.
T1  - Central insulin action regulates peripheral glucose and fat metabolism in mice
JF  - The Journal of Clinical Investigation (JCI)
Y1  - 2008
U6  - http://dx.doi.org/10.1172/JCI31073
SN  - 1558-8238
VL  - 118
IS  - 6
SP  - 2132
EP  - 2147
ER  - 
TY  - JOUR
A1  - Prielmeier, Franz
A1  - Nagatomo, Yasushi
A1  - Frahm, Jens
T1  - Cerebral blood oxygenation in rat brain during hypoxic hypoxia. Quantitative MRI of effective transverse relaxation rates
JF  - Magnetic Resonance in Medicine. 31 (1994), H. 6
Y1  - 1994
SN  - 0740-3194
SP  - 678
EP  - 681
ER  - 
TY  - JOUR
A1  - Lassonczyk, Beate
T1  - Characteristics of a fossil soil developed on the basement complex in the northeast Somalia
JF  - Berliner geowissenschaftliche Abhandlungen / Reihe A, Geologie und Paläontologie / hrsg. von d. Geowissenschaftlichen Instituten der Freien u. d. Technischen Universität Berlin. 120 A (1990)
Y1  - 1990
SN  - 0172-8784
SP  - 713
EP  - 718
ER  - 
TY  - CHAP
A1  - Roth, J.
A1  - Möhring, S.
A1  - Tippkötter, Nils
T1  - Characterization and evaluation of lignocellulosic biomass 130 hydrolysates for ABE fermentation
T2  - New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany
Y1  - 2016
SP  - 130
PB  - DECHEMA
CY  - Frankfurt am Main
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Kim, Jihoe
A1  - Darley, Daniel
A1  - Buckel, Wolfgang
T1  - Characterization of (R)-2-hydroxyisocaproate dehydrogenase and a family III coenzyme A transferase involved in reduction of L-leucine to isocaproate by Clostridium difficile / Kim, J. ; Darley, D. ; Selmer, T. ; Buckel, W.
JF  - Applied and Environmental Microbiology. 72 (2006), H. 9
Y1  - 2006
SN  - 0099-2240
N1  - PMID: 16957230
SP  - 6062
EP  - 6069
ER  - 
TY  - JOUR
A1  - Jossek, Ralf
A1  - Bongaerts, Johannes
A1  - Sprenger, Georg A.
T1  - Characterization of a new feedback-resistant 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase AroF of Escherichia coli
JF  - FEMS microbiology letters
Y1  - 2001
SN  - 1574-6968
VL  - Vol. 202
IS  - Iss. 1
SP  - 145
EP  - 148
ER  - 
TY  - JOUR
A1  - Kusch, Peter
A1  - Rieser, Claudia
A1  - Knupp, Gerd
A1  - Mang, Thomas
T1  - Characterization of copolymers of methacrylic acid with poly(ethylene glycol) methyl ether methacrylate macromonomers by analytical pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS)
JF  - Journal of Analytical and Applied Pyrolysis
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.jaap.2015.03.003
SN  - 0165-2370
VL  - Vol. 113
SP  - 412
EP  - 418
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Rechmann, Hanrik
A1  - Friedrich, Andrea
A1  - Forouzan, Dara
T1  - Characterization of photosynthetically active duckweed ( Wolffia australiana ) in vitro culture by Respiration Activity Monitoring System (RAMOS) / Henrik Rechmann, Andrea Friedrich, Dara Forouzan, Stefan Barth, Heide Schnabl, Manfred Biselli und Robert B
JF  - Biotechnology Letters. 29 (2007), H. 6
Y1  - 2007
SN  - 0141-5492
SP  - 971
EP  - 977
ER  - 
TY  - JOUR
A1  - Wardoyo, Arinto Y.P.
A1  - Noor, Johan A.E.
A1  - Elbers, Gereon
A1  - Schmitz, Sandra
A1  - Flaig, Sascha T.
A1  - Budianto, Arif
T1  - Characterizing volcanic ash elements from the 2015 eruptions of bromo and raung volcanoes, Indonesia
JF  - Polish Journal of Environmental Studies
N2  - The volcanic eruptions of Mt. Bromo and Mt. Raung in East Java, Indonesia, in 2015 perturbed volcanic materials and affected surface-layer air quality at surrounding locations. During the episodes, the volcanic ash from the eruptions influenced visibility, traffic accidents, flight schedules, and human health. In this research, the volcanic ash particles were collected and characterized by relying on the detail of physical observation. We performed an assessment of the volcanic ash elements to characterize the volcanic ash using two different methods which are aqua regia extracts followed by MP-AES and XRF laboratory test of bulk samples. The analysis results showed that the volcanic ash was mixed of many materials, such as Al, Si, P, K, Ca, Ti, V, Cr, Mn, Fe, Ni, and others. Fe, Si, Ca, and Al were found as the major elements, while the others were the trace elements Ba, Cr, Cu, Mn, P, Mn, Ni, Zn, Sb, Sr, and V with the minor concentrations. XRF analyses showed that Fe dominated the elements of the volcanic ash. The XRF analysis showed that Fe was at 35.40% in Bromo and 43.00% in Raung of the detected elements in bulk material. The results of aqua regia extracts analyzed by MP-AES were 1.80% and 1.70% of Fe element for Bromo and Raung volcanoes, respectively.
Y1  - 2020
U6  - http://dx.doi.org/10.15244/pjoes/99101
SN  - 2083-5906
VL  - 29
IS  - 2
SP  - 1899
EP  - 1907
PB  - HARD
CY  - Olsztyn
ER  - 
TY  - JOUR
A1  - Kotter, Michael
A1  - Lovera, P.
A1  - Riekert, L.
T1  - Charakterisierung eines Trägerkatalysators durch effektive Diffusionskoeffizienten, Labyrinthfaktor und mittlere Porenradien
JF  - Berichte der Bunsen-Gesellschaft für Physikalische Chemie
N2  - Der effektive Diffusionskoeffizient Deff von Gasen in einem porösen Medium hängt im Knudsen- bzw. Übergangsgebiet vom effektiven Porenradius 〈r〉 der Struktur ab. 〈r〉 kann aus Deff ermittelt werden, sofern der Labyrinthfaktor χ der Struktur aus der Kinetik des Stoffaustausches in den mit einer flüssigen Phase gefüllten Poren separat bestimmt wird. An einem Trägerkatalysator (CuO auf γ-Al₂O₃) ergab sich auf diese Weise ein für die Diffusion maßgebender effektiver Porenradius, der in den Bereich der Makroporen fällt. Ein Vergleich mit dem aus reaktionskinetischen Daten ermittelten effektiven Diffusionskoeffizienten läßt auf eine ungleichmäßige Verteilung der aktiven Komponente im Kontaktkorn schließen.
Y1  - 1976
SN  - 0005-9021
U6  - http://dx.doi.org/10.1002/bbpc.19760800112
VL  - 80
IS  - 1
SP  - 61
EP  - 66
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Jahnke, J.
T1  - Chemical and Physical Effects on the Shape and Growth of the Diatom Biddulphia sinensis GREVILLE in Batch Cultures: A Contribution to Bioindication in Plankton Ecology / Jahnke, J. ; Baumann, M.
JF  - Hydrobiological Bulletin . 17 (1983), H. 1
Y1  - 1983
SN  - 0165-1404
SP  - 5
EP  - 20
ER  - 
TY  - CHAP
A1  - Muffler, Kai
A1  - Tippkötter, Nils
A1  - Ulber, Roland
ED  - Timmis, Kenneth N.
T1  - Chemical feedstocks and fine chemicals from other substrates
T2  - Handbook of hydrocarbon and lipid microbiology. Volume 4:  Consequences of microbial interactions with hydrocarbons, oils and lipids. - (Springer reference)
Y1  - 2010
SN  - 978-3-540-77588-1
U6  - http://dx.doi.org/10.1007%2F978-3-540-77587-4_214
SP  - 2891
EP  - 2902
PB  - Springer
CY  - Berlin [u.a.]
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Zimmermann, H. P.
A1  - Gober, M. K.
A1  - Kratz, J. V.
T1  - Chemical Properties of Element 105 in Aqueous Solution: Back Extraction from Triisooctyl Amine into 0.5M HCl / H.P. Zimmermann, M.K. Gober, J.V. Kratz, M. Schädel, E. Schimpf, K.E. Gregorich, A. Türler, K.R. Czerwinski, N.J. Hannink, B. Kadkhodayan, D.M.
JF  - Radiochimica Acta. 60 (1993)
Y1  - 1993
SN  - 0033-8230
SP  - 11
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Schädel, M.
A1  - Brüchle, W.
A1  - Schimpf, E.
T1  - Chemical Properties of Element 105 in Aqueous Solution: Cation Exchange Separations with α-Hydroxyisobutyric Acid / M. Schädel, W. Brüchle, E. Schimpf, H.P. Zimmermann, M.K. Gober, J.V. Kratz,  N. Trautmann, H. Gäggeler, D. Jost, J. Kovacs, U.W. Sche
JF  - Radiochimica Acta. 57 (1992)
Y1  - 1992
SN  - 0033-8230
SP  - 85
EP  - 92
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Gober, M. K.
A1  - Kratz, J. V.
A1  - Zimmermann, H. P.
T1  - Chemical Properties of Element 105 in Aqueous Solution: Extractions into Diisobutylcarbinol / M.K. Gober, J.V. Kratz, H.P. Zimmermann, M. Schädel, W. Brüchle, E. Schimpf, K.E. Gregorich, A. Türler, N.J. Hannink, K.R. Czerwinski, B. Kadkhodayan, D.M. Lee,
JF  - Radiochimica Acta. 57 (1992)
Y1  - 1992
SN  - 0033-8230
SP  - 77
EP  - 84
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Kratz, J. V.
A1  - Zimmermann, H. P.
A1  - Schädel, M.
T1  - Chemical Properties of Element 105 in Aqueous Solutions: Halide Complex Formation and Anion Exchange into Triisooctylamine / J.V. Kratz, H.P. Zimmermann, U.W. Scherer, M. Schädel, W. Brüchle, K.E. Gregorich, C.M. Gannett, H.L. Hall, R.A. Henderson, D.M. L
JF  - Radiochimica Acta. 48 (1989)
Y1  - 1989
SN  - 0033-8230
SP  - 121
ER  - 
TY  - JOUR
A1  - Dünnwald, Thomas
A1  - Demir, Ayhan S.
A1  - Siegert, Petra
A1  - Pohl, Martina
A1  - Müller, Michael
T1  - ChemInform Abstract: Enantioselective synthesis of (S)-2-Hydroxypropanone derivatives by Benzoylformate Decarboxylase Catalyzed C—C Bond Formation
JF  - Cheminform
Y1  - 2001
SN  - 1522-2667 (E-Journal); 0931-7597 (Print)
VL  - Vol. 32
IS  - Iss. 4
SP  - Publ. online
ER  - 
TY  - JOUR
A1  - Bäcker, Matthias
A1  - Rakowski, D.
A1  - Poghossian, Arshak
A1  - Biselli, Manfred
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Chip-based amperometric enzyme sensor system for monitoring of bioprocesses by flow-injection analysis
JF  - Journal of Biotechnology
N2  - A microfluidic chip integrating amperometric enzyme sensors for the detection of glucose, glutamate and glutamine in cell-culture fermentation processes has been developed. The enzymes glucose oxidase, glutamate oxidase and glutaminase were immobilized by means of cross-linking with glutaraldehyde on platinum thin-film electrodes integrated within a microfluidic channel. The biosensor chip was coupled to a flow-injection analysis system for electrochemical characterization of the sensors. The sensors have been characterized in terms of sensitivity, linear working range and detection limit. The sensitivity evaluated from the respective peak areas was 1.47, 3.68 and 0.28 μAs/mM for the glucose, glutamate and glutamine sensor, respectively. The calibration curves were linear up to a concentration of 20 mM glucose and glutamine and up to 10 mM for glutamate. The lower detection limit amounted to be 0.05 mM for the glucose and glutamate sensor, respectively, and 0.1 mM for the glutamine sensor. Experiments in cell-culture medium have demonstrated a good correlation between the glutamate, glutamine and glucose concentrations measured with the chip-based biosensors in a differential-mode and the commercially available instrumentation. The obtained results demonstrate the feasibility of the realized microfluidic biosensor chip for monitoring of bioprocesses.
Y1  - 2013
U6  - http://dx.doi.org/10.1016/j.jbiotec.2012.03.014
SN  - 0168-1656
VL  - 163
IS  - 4
SP  - 371
EP  - 376
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Kuropka, Rolf
A1  - Müller, Bettina
A1  - Höcker, Hartwig
A1  - Berndt, Heinz
T1  - Chiral stationary phases via hydrosilylation reaction of N-acryloylamino acids : I. Stationary phase with one chiral centre for high-performance liquid chromatography and development of a new derivatization pattern for amino acid enantiomers
JF  - Journal of chromatography A
Y1  - 1989
SN  - 0021-9673
IS  - 481
SP  - 380
EP  - 386
ER  - 
TY  - JOUR
A1  - Zhang, Jin
A1  - Heimbach, Tycho
A1  - Scheer, Nico
A1  - Barve, Avantika
A1  - Li, Wenkui
A1  - Lin, Wen
A1  - He, Handan
T1  - Clinical Exposure Boost Predictions by Integrating Cytochrome P450 3A4–Humanized Mouse Studies With PBPK Modeling
JF  - Journal of Pharmaceutical Sciences
N2  - NVS123 is a poorly water-soluble protease 56 inhibitor in clinical development. Data from in vitro hepatocyte studies suggested that NVS123 is mainly metabolized by CYP3A4. As a consequence of limited solubility, NVS123 therapeutic plasma exposures could not be achieved even with high doses and optimized formulations. One approach to overcome NVS123 developability issues was to increase plasma exposure by coadministrating it with an inhibitor of CYP3A4 such as ritonavir. A clinical boost effect was predicted by using physiologically based pharmacokinetic (PBPK) modeling. However, initial boost predictions lacked sufficient confidence because a key parameter, fraction of drug metabolized by CYP3A4 (ƒₘCYP3A4), could not be estimated with accuracy on account of disconnects between in vitro and in vivo preclinical data. To accurately estimate ƒₘCYP3A4 in human, an in vivo boost effect study was conducted using CYP3A4-humanized mouse model which showed a 33- to 56-fold exposure boost effect. Using a top-down approach, human ƒₘCYP3A4 for NVS123 was estimated to be very high and included in the human PBPK modeling to support subsequent clinical study design. The combined use of the in vivo boost study in CYP3A4-humanized mouse model mice along with PBPK modeling accurately predicted the clinical outcome and identified a significant NVS123 exposure boost (∼42-fold increase) with ritonavir.
Y1  - 2016
U6  - http://dx.doi.org/doi.org/10.1016/j.xphs.2016.01.021
SN  - 0022-3549
VL  - Volume 105
IS  - Issue 4
SP  - 1398
EP  - 1404
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Engel, Mareike
A1  - Gemünde, Andre
A1  - Holtmann, Dirk
A1  - Müller-Renno, Christine
A1  - Ziegler, Christiane
A1  - Tippkötter, Nils
A1  - Ulber, Roland
T1  - Clostridium acetobutylicum’s connecting world: cell appendage formation in bioelectrochemical systems
JF  - ChemElectroChem
Y1  - 2019
U6  - http://dx.doi.org/10.1002/celc.201901656
SN  - 2196-0216
IS  - Accepted Article
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - PAT
A1  - Lauth, Jakob
A1  - Hoelderich, Wolfgang
A1  - Harth, Klaus
A1  - Hibst, Hartmuth
T1  - Coated catalysts : United States Patent ; patent number 5,559,065 ; date of patent: Sep. 24, 1996 / assignee: BASF Aktiengesellschaft
Y1  - 1996
N1  - Barcode: US005559065A ; Volltext über Datenbank: http://www.google.com/patents
PB  - [United States Trademark and Patent Office]
CY  - [Alexandria, VA]
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Schröder, B.
A1  - Herfurth, C.
A1  - Schmoll, H.-J.
T1  - Cocultivation of hematopoietic cells in a fluidized bed reactor / Schröder, B. ; Herfurth, C. ; Biselli, M. ; Schmoll, H.-J. ; Link, H. ; Ebell, W. ; Wandrey, C.
JF  - Animal cell technology : basic & applied aspects : proceedings of the Eighth Annual Meeting of the Japanese Association for Animal Cell Technology, Iizuka, Fukuoka, Japan, November 6-10, 1995 / edited by K. Funatsu, Y. Shirai, and T. Matsushita
Y1  - 1997
SN  - 0-7923-4486-3
N1  - Japanese Association for Animal Cell Technology. ; Meeting ; <8 ; 1995 : ; Iizuka-shi, Japan>
SP  - 137
EP  - 141
PB  - Kluwer Acad. Press
CY  - Boston
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Gäggeler, H. W.
A1  - Jost, D. T.
A1  - Türler, A.
T1  - Cold Fusion Reactions with 48Ca / H.W. Gäggeler, D.T. Jost, A. Türler, P. Armbruster, W. Brüchle, H. Folger, F.P. Heßberger, S. Hofmann, G. Münzenberg, V. Ninov, W. Reisdorf, M. Schädel, K. Sümmerer, J.V. Kratz, U. Scherer, M.E. Leino
JF  - Nuclear Physics A . 502 (1989), H. 1
Y1  - 1989
SN  - 0375-9474
SP  - 561
EP  - 570
ER  - 
TY  - PAT
A1  - Lauth, Jakob
A1  - Müller, Ulrich
A1  - Hoelderich, Wolfgang
T1  - Colored crystalline aluminophosphates and/or silicoaluminophosphates of the AEL or VFI type : United States Patent ; patent number 5,360,474 ; date of patent: Nov. 1, 1994 / assignee: BASF Aktiengesellschaft
Y1  - 1994
N1  - Barcode: US005360474A ; Volltext über Datenbank: http://www.google.com/patents
PB  - [United States Trademark and Patent Office]
CY  - [Alexandria, VA]
ER  - 
TY  - JOUR
A1  - Kappler-Tanudyaya, Nathalie
A1  - Schmitt, Heike
A1  - Tippkötter, Nils
A1  - Meyer, Lina
A1  - Lenzen, Sigurd
A1  - Ulber, Roland
T1  - Combination of biotransformation and chromatography for the isolation and purification of mannoheptulose
JF  - Biotechnology Journal
N2  - Mannoheptulose is a seven-carbon sugar. It is an inhibitor of glucose-induced insulin secretion due to its ability to selectively inhibit the enzyme glucokinase. An improved procedure for mannoheptulose isolation from avocados is described in this study (based upon the original method by La Forge). The study focuses on the combination of biotransformation and downstream processing (preparative chromatography) as an efficient method to produce a pure extract of mannoheptulose. The experiments were divided into two major phases. In the first phase, several methods and parameters were compared to optimize the mannoheptulose extraction with respect to efficiency and purity. In the second phase, a mass balance of mannoheptulose over the whole extraction process was undertaken to estimate the yield and efficiency of the total extraction process. The combination of biotransformation and preparative chromatography allowed the production of a pure mannoheptulose extract. In a biological test, the sugar inhibited the glucokinase enzyme activity efficiently.
Y1  - 2007
U6  - http://dx.doi.org/10.1002/biot.200700004
SN  - 1860-7314
VL  - 2
IS  - 6
SP  - 692
EP  - 699
ER  - 
TY  - JOUR
A1  - Schiffels, Johannes
A1  - Selmer, Thorsten
T1  - Combinatorial assembly of ferredoxin‐linked modules in Escherichia coli yields a testing platform for Rnf‐complexes
JF  - Biotechnology and Bioengineering
Y1  - 2019
U6  - http://dx.doi.org/10.1002/bit.27079
IS  - accepted article
SP  - 1
EP  - 36
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Balakrishnan, Karthikeyan
A1  - Andrei-Selmer, Luminita-Cornelia
A1  - Selmer, Thorsten
A1  - Bacher, Michael
A1  - Dodel, Richard
T1  - Comparison of Intravenous Immunoglobulins for Naturally Occurring Autoantibodies against Amyloid-β
JF  - Journal of Alzheimer's Disease
Y1  - 2010
SN  - 1387-2877
VL  - 20
IS  - 1
SP  - 135
EP  - 143
ER  - 
TY  - BOOK
A1  - Rath, Walter
A1  - Koch, Markus
A1  - Reichardt, Rüdiger
T1  - Composition de polyurethanne et joint d’etancheite pour vitrage = Polyurethanzusammensetzung und Verglasungsdichtungs-Profil / Rath, Walter, Koch, Markus ; Reichardt, Rüdiger [Erfinder]
Y1  - 2002
N1  - EP0789719 24.01.2002 Veröffentlichungstag im Patentblatt  ; Volltext recherchierbar über
PB  - Europäisches Patentamt
CY  - München
ER  - 
TY  - JOUR
A1  - Wulfhorst, Helene
A1  - Duwe, Anna-Maria
A1  - Merseburg, Johannes
A1  - Tippkötter, Nils
T1  - Compositional analysis of pretreated (beech) wood using differential scanning calorimetry and multivariate data analysis
JF  - Tetrahedron
N2  - The composition of plant biomass varies depending on the feedstock and pre-treatment conditions and influences its processing in biorefineries. In order to ensure optimal process conditions, the quantitative proportion of the main polymeric components of the pre-treated biomass has to be determined. Current standard procedures for biomass compositional analysis are complex, the measurements are afflicted with errors and therefore often not comparable. Hence, new powerful analytical methods are urgently required to characterize biomass. In this contribution, Differential Scanning Calorimetry (DSC) was applied in combination with multivariate data analysis (MVA) to detect the cellulose content of the plant biomass pretreated by Liquid Hot Water (LHW) and Organosolv processes under various conditions. Unlike conventional techniques, the developed analytic method enables the accurate quantification of monosaccharide content of the plant biomass without any previous sample preparation. It is easy to handle and avoids errors in sample preparation.
Y1  - 2016
U6  - http://dx.doi.org/10.1016/j.tet.2016.04.029
VL  - 72
IS  - 46
SP  - 7329
EP  - 7334
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Hager, Jonathan
A1  - Hentschke, Reinhard
A1  - Hojdis, Nils
A1  - Karimi-Varzaneh, Hossein Ali
T1  - Computer Simulation of Particle–Particle Interaction in a Model Polymer Nanocomposite
JF  - Macromolecules
Y1  - 2015
U6  - http://dx.doi.org/10.1021/acs.macromol.5b01864
SN  - 1520-5835
VL  - 48
IS  - 24
SP  - 9039
EP  - 9049
ER  - 
TY  - JOUR
A1  - Bäcker, Matthias
A1  - Beging, Stefan
A1  - Biselli, Manfred
A1  - Poghossian, Arshak
A1  - Wang, J.
A1  - Zang, Werner
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Concept for a solid-state multi-parameter sensor system for cell-culture monitoring
JF  - Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI
Y1  - 2009
SN  - 0013-4686
SP  - 6107
EP  - 6112
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Haeger, Gerrit
A1  - Grankin, Alina
A1  - Wagner, Michaela
T1  - Construction of an Aspergillus oryzae triple amylase deletion mutant as a chassis to evaluate industrially relevant amylases using multiplex CRISPR/Cas9 editing technology
JF  - Applied Research
N2  - Aspergillus oryzae is an industrially relevant organism for the secretory production of heterologous enzymes, especially amylases. The activities of potential heterologous amylases, however, cannot be quantified directly from the supernatant due to the high background activity of native α-amylase. This activity is caused by the gene products of amyA, amyB, and amyC. In this study, an in vitro CRISPR/Cas9 system was established in A. oryzae to delete these genes simultaneously. First, pyrG of A. oryzae NSAR1 was mutated by exploiting NHEJ to generate a counter-selection marker. Next, all amylase genes were deleted simultaneously by co-transforming a repair template carrying pyrG of Aspergillus nidulans and flanking sequences of amylase gene loci. The rate of obtained triple knock-outs was 47%. We showed that triple knockouts do not retain any amylase activity in the supernatant. The established in vitro CRISPR/Cas9 system was used to achieve sequence-specific knock-in of target genes. The system was intended to incorporate a single copy of the gene of interest into the desired host for the development of screening methods. Therefore, an integration cassette for the heterologous Fpi amylase was designed to specifically target the amyB locus. The site-specific integration rate of the plasmid was 78%, with exceptional additional integrations. Integration frequency was assessed via qPCR and directly correlated with heterologous amylase activity. Hence, we could compare the efficiency between two different signal peptides. In summary, we present a strategy to exploit CRISPR/Cas9 for gene mutation, multiplex knock-out, and the targeted knock-in of an expression cassette in A. oryzae. Our system provides straightforward strain engineering and paves the way for development of fungal screening systems.
KW  - aspergillus
KW  - CRISPR/Cas9
KW  - filamentous fungi
KW  - genome engineering
Y1  - 2023
U6  - http://dx.doi.org/10.1002/appl.202200106
SN  - 2702-4288
IS  - Early View
SP  - 1
EP  - 15
PB  - Wiley-VCH
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
T1  - Controlled ion track etching / J. George;  M. Irkens ;  S. Neumann ;  U. W. Scherer ;  A. Srivastava ;  D. Sinha ; D. Fink
JF  - Radiation Effects and Defects in Solids. 161 (2006), H. 3
Y1  - 2006
SP  - 161
EP  - 175
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Lukatela, G.
A1  - Krauss, N.
A1  - Theis, K.
T1  - Crystal structure of human arylsulfatase A: the aldehyde function and the metal ion at the active site suggest a novel mechanism for sulfate ester hydrolysis / Lukatela, G. ; Krauss, N. ; Theis, K. ; Selmer, T. ; Gieselmann, V. ; Figura, K. von ; Saenger,
JF  - Biochemistry. 37 (1998), H. 11
Y1  - 1998
SP  - 3654
EP  - 3664
ER  - 
TY  - JOUR
A1  - Hemmerling, H.-J.
A1  - Merschenz-Quack, Angelika
A1  - Wunderlich, H.
T1  - Crystal structures of indeno[1,2-d]imidazoles. XIth European Crystallographic Meeting, Vienna 1988
JF  - Zeitschrift für Kristallographie - Crystalline Materials
Y1  - 1988
SN  - 2196-7105 (E-Books); 2194-4946 (Print)
VL  - 185
IS  - H. 1-4
SP  - 256
ER  - 
TY  - JOUR
A1  - Lauth, Jakob
A1  - Hoelderich, W.
A1  - Wagenblast, G.
T1  - Crystalline zeolites containing indigo dyes
JF  - Zeolites. 15 (1995), H. 1
Y1  - 1995
SN  - 0144-2449
SP  - 86
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Noll, Thomas
A1  - Jelinek, Nanni
A1  - Schmidt, Sebastian
T1  - Cultivation of Hematopoietic Stem and Progenitor Cells: biochemical Engineering Aspects / Thomas Noll, Nanni Jelinek, Sebastian Schmidt, Manfred Biselli und Christian Wandrey
JF  - Tools and Applications of Biochemical Engineering Science
Y1  - 2002
SN  - 3-540-42250-1
N1  - Advances in Biochemical Engineering/Biotechnology. 74
SP  - 111
EP  - 128
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Hilbert, U.
A1  - Noll, T.
T1  - Cultivation of Human HCMV Specific Lymphocytes – An Example for  Adoptive Immunotherapy / Hilbert, U. ; Biselli, M. ; Noll, T.
JF  - Animal cell technology : from target to market ; Tylösand, Sweden, June 10 - 14, 2001 / ed. by E. Lindner-Olsson ...
Y1  - 2001
SN  - 1-4020-0264-5
N1  - Proceedings of the ... ESACT meeting ; 17
SP  - 558
EP  - 561
PB  - Kluwer
CY  - Dordrecht
ER  - 
TY  - JOUR
A1  - Schnitzler, Thomas
T1  - Cultivation of hybridoma cell line CF-10H5 (DSMZ ACC477)
JF  - Application notes / Sartorius stedim biotech
Y1  - 2009
SP  - 1
EP  - 4
ER  - 
TY  - JOUR
A1  - Schnitzler, Thomas
A1  - Biselli, Manfred
T1  - Cultivo de lineas celulares de hibridoma por CF-10H5 (DSMZ ACC477) en el BIOSTAT B plus
JF  - Noticias tecnicas del laboratorio
Y1  - 2005
VL  - 13
IS  - 3
SP  - 7
EP  - 8
ER  - 
TY  - BOOK
A1  - Lauth, Jakob
A1  - Dingerdissen, Uwe
A1  - Steuerle, Ulrich
T1  - Cup catalyst and process for producing aziridines : [Internationale Pantentanmeldung WO9633018] ; Veröffentlichungsdatum: 1996-10-24 / Anmelder: BASF AG ; Dingerdissen, Uwe ; Lauth, Guenther ; Steuerle, Ulrich. Erfinder: Dingerdissen, Uwe ; Lauth, Guenther ; Steuerle, Ulrich
Y1  - 1996
PB  - [Weltorganisation für geistiges Eigentum]
CY  - [Genf]
ER  - 
TY  - JOUR
A1  - Henderson, Colin J.
A1  - Mclaughlin, Lesley A.
A1  - Scheer, Nico
A1  - Stanley, Lesley A.
A1  - Wolf, C. Roland
T1  - Cytochrome b5 Is a Major Determinant of Human Cytochrome P450 CYP2D6 and CYP3A4 Activity In Vivo s
JF  - Molecular Pharmacology
Y1  - 2015
U6  - http://dx.doi.org/10.1124/mol.114.097394
SN  - 1521-0111
VL  - 87
IS  - 4
SP  - 733
EP  - 739
PB  - ASPET
CY  - Bethesda
ER  - 
TY  - JOUR
A1  - Nokihara, Kiyoshi
A1  - Berndt, Heinz
T1  - Darstellung von Bis(S-methoxycarbonylthio)-B-Kette des Rinderinsulins
JF  - Hoppe-Seyler's Zeitschrift für physiologische Chemie
Y1  - 1979
U6  - http://dx.doi.org/10.1515/bchm2.1979.360.1.773
SN  - 1437-4315
SN  - 0018-4888
VL  - 360
IS  - 1
SP  - 773
EP  - 776
ER  - 
TY  - JOUR
A1  - Pinkenburg, Olaf
A1  - Schiffels, Johannes
A1  - Selmer, Thorsten
T1  - Das CoLibry-Konzept – ein Werkzeugkasten für die Synthetische Biologie: Bioproduktion
JF  - BIOspektrum
N2  - Regardless of size or destination, synthetic biology starts with com-parably small information units, which need to be combined and properly arranged in order to achieve a certain goal. This may be the de novo synthesis of individual genes from oligonucleotides, a shuffling of protein domains in order to create novel biocatalysts, the assembly of multiple enzyme encoding genes in metabolic pathway design, or strain development at the production stage. The CoLibry concept has been designed in order to close the gap between recombinant production of individual genes and genome editing.
Y1  - 2016
U6  - http://dx.doi.org/10.1007/s12268-016-0734-8
VL  - 22
IS  - 6
SP  - 593
EP  - 595
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Thomzik, Manfred
T1  - Das Entstehen geruchsintensiver schwefelhaltiger organischer Verbindungen in Lackierereien und Möglichkeiten zu deren Vermeidung
JF  - Aus der Tätigkeit der LIS / Landesanstalt für Immissionsschutz des Landes Nordrhein-Westfalen. 1989 (1990)
Y1  - 1990
SN  - 0931-5497
SP  - 35
ER  -