TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Kratz, J. V.
A1  - Zimmermann, H. P.
A1  - Schädel, M.
T1  - Chemical Properties of Element 105 in Aqueous Solutions: Halide Complex Formation and Anion Exchange into Triisooctylamine / J.V. Kratz, H.P. Zimmermann, U.W. Scherer, M. Schädel, W. Brüchle, K.E. Gregorich, C.M. Gannett, H.L. Hall, R.A. Henderson, D.M. L
JF  - Radiochimica Acta. 48 (1989)
Y1  - 1989
SN  - 0033-8230
SP  - 121
ER  - 
TY  - JOUR
A1  - Dünnwald, Thomas
A1  - Demir, Ayhan S.
A1  - Siegert, Petra
A1  - Pohl, Martina
A1  - Müller, Michael
T1  - ChemInform Abstract: Enantioselective synthesis of (S)-2-Hydroxypropanone derivatives by Benzoylformate Decarboxylase Catalyzed C—C Bond Formation
JF  - Cheminform
Y1  - 2001
SN  - 1522-2667 (E-Journal); 0931-7597 (Print)
VL  - Vol. 32
IS  - Iss. 4
SP  - Publ. online
ER  - 
TY  - JOUR
A1  - Bäcker, Matthias
A1  - Rakowski, D.
A1  - Poghossian, Arshak
A1  - Biselli, Manfred
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Chip-based amperometric enzyme sensor system for monitoring of bioprocesses by flow-injection analysis
JF  - Journal of Biotechnology
N2  - A microfluidic chip integrating amperometric enzyme sensors for the detection of glucose, glutamate and glutamine in cell-culture fermentation processes has been developed. The enzymes glucose oxidase, glutamate oxidase and glutaminase were immobilized by means of cross-linking with glutaraldehyde on platinum thin-film electrodes integrated within a microfluidic channel. The biosensor chip was coupled to a flow-injection analysis system for electrochemical characterization of the sensors. The sensors have been characterized in terms of sensitivity, linear working range and detection limit. The sensitivity evaluated from the respective peak areas was 1.47, 3.68 and 0.28 μAs/mM for the glucose, glutamate and glutamine sensor, respectively. The calibration curves were linear up to a concentration of 20 mM glucose and glutamine and up to 10 mM for glutamate. The lower detection limit amounted to be 0.05 mM for the glucose and glutamate sensor, respectively, and 0.1 mM for the glutamine sensor. Experiments in cell-culture medium have demonstrated a good correlation between the glutamate, glutamine and glucose concentrations measured with the chip-based biosensors in a differential-mode and the commercially available instrumentation. The obtained results demonstrate the feasibility of the realized microfluidic biosensor chip for monitoring of bioprocesses.
Y1  - 2013
U6  - http://dx.doi.org/10.1016/j.jbiotec.2012.03.014
SN  - 0168-1656
VL  - 163
IS  - 4
SP  - 371
EP  - 376
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Kuropka, Rolf
A1  - Müller, Bettina
A1  - Höcker, Hartwig
A1  - Berndt, Heinz
T1  - Chiral stationary phases via hydrosilylation reaction of N-acryloylamino acids : I. Stationary phase with one chiral centre for high-performance liquid chromatography and development of a new derivatization pattern for amino acid enantiomers
JF  - Journal of chromatography A
Y1  - 1989
SN  - 0021-9673
IS  - 481
SP  - 380
EP  - 386
ER  - 
TY  - JOUR
A1  - Zhang, Jin
A1  - Heimbach, Tycho
A1  - Scheer, Nico
A1  - Barve, Avantika
A1  - Li, Wenkui
A1  - Lin, Wen
A1  - He, Handan
T1  - Clinical Exposure Boost Predictions by Integrating Cytochrome P450 3A4–Humanized Mouse Studies With PBPK Modeling
JF  - Journal of Pharmaceutical Sciences
N2  - NVS123 is a poorly water-soluble protease 56 inhibitor in clinical development. Data from in vitro hepatocyte studies suggested that NVS123 is mainly metabolized by CYP3A4. As a consequence of limited solubility, NVS123 therapeutic plasma exposures could not be achieved even with high doses and optimized formulations. One approach to overcome NVS123 developability issues was to increase plasma exposure by coadministrating it with an inhibitor of CYP3A4 such as ritonavir. A clinical boost effect was predicted by using physiologically based pharmacokinetic (PBPK) modeling. However, initial boost predictions lacked sufficient confidence because a key parameter, fraction of drug metabolized by CYP3A4 (ƒₘCYP3A4), could not be estimated with accuracy on account of disconnects between in vitro and in vivo preclinical data. To accurately estimate ƒₘCYP3A4 in human, an in vivo boost effect study was conducted using CYP3A4-humanized mouse model which showed a 33- to 56-fold exposure boost effect. Using a top-down approach, human ƒₘCYP3A4 for NVS123 was estimated to be very high and included in the human PBPK modeling to support subsequent clinical study design. The combined use of the in vivo boost study in CYP3A4-humanized mouse model mice along with PBPK modeling accurately predicted the clinical outcome and identified a significant NVS123 exposure boost (∼42-fold increase) with ritonavir.
Y1  - 2016
U6  - http://dx.doi.org/doi.org/10.1016/j.xphs.2016.01.021
SN  - 0022-3549
VL  - Volume 105
IS  - Issue 4
SP  - 1398
EP  - 1404
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Engel, Mareike
A1  - Gemünde, Andre
A1  - Holtmann, Dirk
A1  - Müller-Renno, Christine
A1  - Ziegler, Christiane
A1  - Tippkötter, Nils
A1  - Ulber, Roland
T1  - Clostridium acetobutylicum’s connecting world: cell appendage formation in bioelectrochemical systems
JF  - ChemElectroChem
Y1  - 2019
U6  - http://dx.doi.org/10.1002/celc.201901656
SN  - 2196-0216
IS  - Accepted Article
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - PAT
A1  - Lauth, Jakob
A1  - Hoelderich, Wolfgang
A1  - Harth, Klaus
A1  - Hibst, Hartmuth
T1  - Coated catalysts : United States Patent ; patent number 5,559,065 ; date of patent: Sep. 24, 1996 / assignee: BASF Aktiengesellschaft
Y1  - 1996
N1  - Barcode: US005559065A ; Volltext über Datenbank: http://www.google.com/patents
PB  - [United States Trademark and Patent Office]
CY  - [Alexandria, VA]
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Schröder, B.
A1  - Herfurth, C.
A1  - Schmoll, H.-J.
T1  - Cocultivation of hematopoietic cells in a fluidized bed reactor / Schröder, B. ; Herfurth, C. ; Biselli, M. ; Schmoll, H.-J. ; Link, H. ; Ebell, W. ; Wandrey, C.
JF  - Animal cell technology : basic & applied aspects : proceedings of the Eighth Annual Meeting of the Japanese Association for Animal Cell Technology, Iizuka, Fukuoka, Japan, November 6-10, 1995 / edited by K. Funatsu, Y. Shirai, and T. Matsushita
Y1  - 1997
SN  - 0-7923-4486-3
N1  - Japanese Association for Animal Cell Technology. ; Meeting ; <8 ; 1995 : ; Iizuka-shi, Japan>
SP  - 137
EP  - 141
PB  - Kluwer Acad. Press
CY  - Boston
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Gäggeler, H. W.
A1  - Jost, D. T.
A1  - Türler, A.
T1  - Cold Fusion Reactions with 48Ca / H.W. Gäggeler, D.T. Jost, A. Türler, P. Armbruster, W. Brüchle, H. Folger, F.P. Heßberger, S. Hofmann, G. Münzenberg, V. Ninov, W. Reisdorf, M. Schädel, K. Sümmerer, J.V. Kratz, U. Scherer, M.E. Leino
JF  - Nuclear Physics A . 502 (1989), H. 1
Y1  - 1989
SN  - 0375-9474
SP  - 561
EP  - 570
ER  - 
TY  - PAT
A1  - Lauth, Jakob
A1  - Müller, Ulrich
A1  - Hoelderich, Wolfgang
T1  - Colored crystalline aluminophosphates and/or silicoaluminophosphates of the AEL or VFI type : United States Patent ; patent number 5,360,474 ; date of patent: Nov. 1, 1994 / assignee: BASF Aktiengesellschaft
Y1  - 1994
N1  - Barcode: US005360474A ; Volltext über Datenbank: http://www.google.com/patents
PB  - [United States Trademark and Patent Office]
CY  - [Alexandria, VA]
ER  - 
TY  - JOUR
A1  - Kappler-Tanudyaya, Nathalie
A1  - Schmitt, Heike
A1  - Tippkötter, Nils
A1  - Meyer, Lina
A1  - Lenzen, Sigurd
A1  - Ulber, Roland
T1  - Combination of biotransformation and chromatography for the isolation and purification of mannoheptulose
JF  - Biotechnology Journal
N2  - Mannoheptulose is a seven-carbon sugar. It is an inhibitor of glucose-induced insulin secretion due to its ability to selectively inhibit the enzyme glucokinase. An improved procedure for mannoheptulose isolation from avocados is described in this study (based upon the original method by La Forge). The study focuses on the combination of biotransformation and downstream processing (preparative chromatography) as an efficient method to produce a pure extract of mannoheptulose. The experiments were divided into two major phases. In the first phase, several methods and parameters were compared to optimize the mannoheptulose extraction with respect to efficiency and purity. In the second phase, a mass balance of mannoheptulose over the whole extraction process was undertaken to estimate the yield and efficiency of the total extraction process. The combination of biotransformation and preparative chromatography allowed the production of a pure mannoheptulose extract. In a biological test, the sugar inhibited the glucokinase enzyme activity efficiently.
Y1  - 2007
U6  - http://dx.doi.org/10.1002/biot.200700004
SN  - 1860-7314
VL  - 2
IS  - 6
SP  - 692
EP  - 699
ER  - 
TY  - JOUR
A1  - Schiffels, Johannes
A1  - Selmer, Thorsten
T1  - Combinatorial assembly of ferredoxin‐linked modules in Escherichia coli yields a testing platform for Rnf‐complexes
JF  - Biotechnology and Bioengineering
Y1  - 2019
U6  - http://dx.doi.org/10.1002/bit.27079
IS  - accepted article
SP  - 1
EP  - 36
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Balakrishnan, Karthikeyan
A1  - Andrei-Selmer, Luminita-Cornelia
A1  - Selmer, Thorsten
A1  - Bacher, Michael
A1  - Dodel, Richard
T1  - Comparison of Intravenous Immunoglobulins for Naturally Occurring Autoantibodies against Amyloid-β
JF  - Journal of Alzheimer's Disease
Y1  - 2010
SN  - 1387-2877
VL  - 20
IS  - 1
SP  - 135
EP  - 143
ER  - 
TY  - BOOK
A1  - Rath, Walter
A1  - Koch, Markus
A1  - Reichardt, Rüdiger
T1  - Composition de polyurethanne et joint d’etancheite pour vitrage = Polyurethanzusammensetzung und Verglasungsdichtungs-Profil / Rath, Walter, Koch, Markus ; Reichardt, Rüdiger [Erfinder]
Y1  - 2002
N1  - EP0789719 24.01.2002 Veröffentlichungstag im Patentblatt  ; Volltext recherchierbar über
PB  - Europäisches Patentamt
CY  - München
ER  - 
TY  - JOUR
A1  - Wulfhorst, Helene
A1  - Duwe, Anna-Maria
A1  - Merseburg, Johannes
A1  - Tippkötter, Nils
T1  - Compositional analysis of pretreated (beech) wood using differential scanning calorimetry and multivariate data analysis
JF  - Tetrahedron
N2  - The composition of plant biomass varies depending on the feedstock and pre-treatment conditions and influences its processing in biorefineries. In order to ensure optimal process conditions, the quantitative proportion of the main polymeric components of the pre-treated biomass has to be determined. Current standard procedures for biomass compositional analysis are complex, the measurements are afflicted with errors and therefore often not comparable. Hence, new powerful analytical methods are urgently required to characterize biomass. In this contribution, Differential Scanning Calorimetry (DSC) was applied in combination with multivariate data analysis (MVA) to detect the cellulose content of the plant biomass pretreated by Liquid Hot Water (LHW) and Organosolv processes under various conditions. Unlike conventional techniques, the developed analytic method enables the accurate quantification of monosaccharide content of the plant biomass without any previous sample preparation. It is easy to handle and avoids errors in sample preparation.
Y1  - 2016
U6  - http://dx.doi.org/10.1016/j.tet.2016.04.029
VL  - 72
IS  - 46
SP  - 7329
EP  - 7334
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Hager, Jonathan
A1  - Hentschke, Reinhard
A1  - Hojdis, Nils
A1  - Karimi-Varzaneh, Hossein Ali
T1  - Computer Simulation of Particle–Particle Interaction in a Model Polymer Nanocomposite
JF  - Macromolecules
Y1  - 2015
U6  - http://dx.doi.org/10.1021/acs.macromol.5b01864
SN  - 1520-5835
VL  - 48
IS  - 24
SP  - 9039
EP  - 9049
ER  - 
TY  - JOUR
A1  - Bäcker, Matthias
A1  - Beging, Stefan
A1  - Biselli, Manfred
A1  - Poghossian, Arshak
A1  - Wang, J.
A1  - Zang, Werner
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Concept for a solid-state multi-parameter sensor system for cell-culture monitoring
JF  - Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI
Y1  - 2009
SN  - 0013-4686
SP  - 6107
EP  - 6112
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Haeger, Gerrit
A1  - Grankin, Alina
A1  - Wagner, Michaela
T1  - Construction of an Aspergillus oryzae triple amylase deletion mutant as a chassis to evaluate industrially relevant amylases using multiplex CRISPR/Cas9 editing technology
JF  - Applied Research
N2  - Aspergillus oryzae is an industrially relevant organism for the secretory production of heterologous enzymes, especially amylases. The activities of potential heterologous amylases, however, cannot be quantified directly from the supernatant due to the high background activity of native α-amylase. This activity is caused by the gene products of amyA, amyB, and amyC. In this study, an in vitro CRISPR/Cas9 system was established in A. oryzae to delete these genes simultaneously. First, pyrG of A. oryzae NSAR1 was mutated by exploiting NHEJ to generate a counter-selection marker. Next, all amylase genes were deleted simultaneously by co-transforming a repair template carrying pyrG of Aspergillus nidulans and flanking sequences of amylase gene loci. The rate of obtained triple knock-outs was 47%. We showed that triple knockouts do not retain any amylase activity in the supernatant. The established in vitro CRISPR/Cas9 system was used to achieve sequence-specific knock-in of target genes. The system was intended to incorporate a single copy of the gene of interest into the desired host for the development of screening methods. Therefore, an integration cassette for the heterologous Fpi amylase was designed to specifically target the amyB locus. The site-specific integration rate of the plasmid was 78%, with exceptional additional integrations. Integration frequency was assessed via qPCR and directly correlated with heterologous amylase activity. Hence, we could compare the efficiency between two different signal peptides. In summary, we present a strategy to exploit CRISPR/Cas9 for gene mutation, multiplex knock-out, and the targeted knock-in of an expression cassette in A. oryzae. Our system provides straightforward strain engineering and paves the way for development of fungal screening systems.
KW  - aspergillus
KW  - CRISPR/Cas9
KW  - filamentous fungi
KW  - genome engineering
Y1  - 2023
U6  - http://dx.doi.org/10.1002/appl.202200106
SN  - 2702-4288
IS  - Early View
SP  - 1
EP  - 15
PB  - Wiley-VCH
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
T1  - Controlled ion track etching / J. George;  M. Irkens ;  S. Neumann ;  U. W. Scherer ;  A. Srivastava ;  D. Sinha ; D. Fink
JF  - Radiation Effects and Defects in Solids. 161 (2006), H. 3
Y1  - 2006
SP  - 161
EP  - 175
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Lukatela, G.
A1  - Krauss, N.
A1  - Theis, K.
T1  - Crystal structure of human arylsulfatase A: the aldehyde function and the metal ion at the active site suggest a novel mechanism for sulfate ester hydrolysis / Lukatela, G. ; Krauss, N. ; Theis, K. ; Selmer, T. ; Gieselmann, V. ; Figura, K. von ; Saenger,
JF  - Biochemistry. 37 (1998), H. 11
Y1  - 1998
SP  - 3654
EP  - 3664
ER  - 
TY  - JOUR
A1  - Hemmerling, H.-J.
A1  - Merschenz-Quack, Angelika
A1  - Wunderlich, H.
T1  - Crystal structures of indeno[1,2-d]imidazoles. XIth European Crystallographic Meeting, Vienna 1988
JF  - Zeitschrift für Kristallographie - Crystalline Materials
Y1  - 1988
SN  - 2196-7105 (E-Books); 2194-4946 (Print)
VL  - 185
IS  - H. 1-4
SP  - 256
ER  - 
TY  - JOUR
A1  - Lauth, Jakob
A1  - Hoelderich, W.
A1  - Wagenblast, G.
T1  - Crystalline zeolites containing indigo dyes
JF  - Zeolites. 15 (1995), H. 1
Y1  - 1995
SN  - 0144-2449
SP  - 86
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Noll, Thomas
A1  - Jelinek, Nanni
A1  - Schmidt, Sebastian
T1  - Cultivation of Hematopoietic Stem and Progenitor Cells: biochemical Engineering Aspects / Thomas Noll, Nanni Jelinek, Sebastian Schmidt, Manfred Biselli und Christian Wandrey
JF  - Tools and Applications of Biochemical Engineering Science
Y1  - 2002
SN  - 3-540-42250-1
N1  - Advances in Biochemical Engineering/Biotechnology. 74
SP  - 111
EP  - 128
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Hilbert, U.
A1  - Noll, T.
T1  - Cultivation of Human HCMV Specific Lymphocytes – An Example for  Adoptive Immunotherapy / Hilbert, U. ; Biselli, M. ; Noll, T.
JF  - Animal cell technology : from target to market ; Tylösand, Sweden, June 10 - 14, 2001 / ed. by E. Lindner-Olsson ...
Y1  - 2001
SN  - 1-4020-0264-5
N1  - Proceedings of the ... ESACT meeting ; 17
SP  - 558
EP  - 561
PB  - Kluwer
CY  - Dordrecht
ER  - 
TY  - JOUR
A1  - Schnitzler, Thomas
T1  - Cultivation of hybridoma cell line CF-10H5 (DSMZ ACC477)
JF  - Application notes / Sartorius stedim biotech
Y1  - 2009
SP  - 1
EP  - 4
ER  - 
TY  - JOUR
A1  - Schnitzler, Thomas
A1  - Biselli, Manfred
T1  - Cultivo de lineas celulares de hibridoma por CF-10H5 (DSMZ ACC477) en el BIOSTAT B plus
JF  - Noticias tecnicas del laboratorio
Y1  - 2005
VL  - 13
IS  - 3
SP  - 7
EP  - 8
ER  - 
TY  - BOOK
A1  - Lauth, Jakob
A1  - Dingerdissen, Uwe
A1  - Steuerle, Ulrich
T1  - Cup catalyst and process for producing aziridines : [Internationale Pantentanmeldung WO9633018] ; Veröffentlichungsdatum: 1996-10-24 / Anmelder: BASF AG ; Dingerdissen, Uwe ; Lauth, Guenther ; Steuerle, Ulrich. Erfinder: Dingerdissen, Uwe ; Lauth, Guenther ; Steuerle, Ulrich
Y1  - 1996
PB  - [Weltorganisation für geistiges Eigentum]
CY  - [Genf]
ER  - 
TY  - JOUR
A1  - Henderson, Colin J.
A1  - Mclaughlin, Lesley A.
A1  - Scheer, Nico
A1  - Stanley, Lesley A.
A1  - Wolf, C. Roland
T1  - Cytochrome b5 Is a Major Determinant of Human Cytochrome P450 CYP2D6 and CYP3A4 Activity In Vivo s
JF  - Molecular Pharmacology
Y1  - 2015
U6  - http://dx.doi.org/10.1124/mol.114.097394
SN  - 1521-0111
VL  - 87
IS  - 4
SP  - 733
EP  - 739
PB  - ASPET
CY  - Bethesda
ER  - 
TY  - JOUR
A1  - Nokihara, Kiyoshi
A1  - Berndt, Heinz
T1  - Darstellung von Bis(S-methoxycarbonylthio)-B-Kette des Rinderinsulins
JF  - Hoppe-Seyler's Zeitschrift für physiologische Chemie
Y1  - 1979
U6  - http://dx.doi.org/10.1515/bchm2.1979.360.1.773
SN  - 1437-4315
SN  - 0018-4888
VL  - 360
IS  - 1
SP  - 773
EP  - 776
ER  - 
TY  - JOUR
A1  - Pinkenburg, Olaf
A1  - Schiffels, Johannes
A1  - Selmer, Thorsten
T1  - Das CoLibry-Konzept – ein Werkzeugkasten für die Synthetische Biologie: Bioproduktion
JF  - BIOspektrum
N2  - Regardless of size or destination, synthetic biology starts with com-parably small information units, which need to be combined and properly arranged in order to achieve a certain goal. This may be the de novo synthesis of individual genes from oligonucleotides, a shuffling of protein domains in order to create novel biocatalysts, the assembly of multiple enzyme encoding genes in metabolic pathway design, or strain development at the production stage. The CoLibry concept has been designed in order to close the gap between recombinant production of individual genes and genome editing.
Y1  - 2016
U6  - http://dx.doi.org/10.1007/s12268-016-0734-8
VL  - 22
IS  - 6
SP  - 593
EP  - 595
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Thomzik, Manfred
T1  - Das Entstehen geruchsintensiver schwefelhaltiger organischer Verbindungen in Lackierereien und Möglichkeiten zu deren Vermeidung
JF  - Aus der Tätigkeit der LIS / Landesanstalt für Immissionsschutz des Landes Nordrhein-Westfalen. 1989 (1990)
Y1  - 1990
SN  - 0931-5497
SP  - 35
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Stahlberg, R.
T1  - Das Thermoselect-Verfahren zur Energie- und Rohstoffgewinnung – Konzepte, Verfahren, Kosten
JF  - Thermische Abfallentsorgung – Konzepte, Kosten, Verfahren. Tagung Veitshöchheim, 27./28.06.1995, VDI-Gesellschaft Energietechnik
Y1  - 1995
SN  - 0083-5560
N1  - VDI-Bericht 1192
SP  - 319
EP  - 319
PB  - VDI-Verl.
CY  - Düsseldorf
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Klose, W.
A1  - Sloboshanin, S.
A1  - Goebel, H. [u.a.]
T1  - Deactivation of a palladium-supported alumina catalyst by hydrogen sulfide during the oxidation of methane
JF  - Langmuir: the ACS journal of surfaces and colloids. 10 (1994), H. 10
Y1  - 1994
SN  - 0743-74363
SP  - 3567
EP  - 3570
ER  - 
TY  - JOUR
A1  - Scheer, Nico
A1  - Kapelyukh, Yury
A1  - Rode, Anja
A1  - Oswald, Stefan
A1  - Busch, Diana
A1  - Mclaughlin, Lesley A.
A1  - Lin, De
A1  - Henderson, Colin J.
A1  - Wolf, C. Roland
T1  - Defining Human Pathways of Drug Metabolism In Vivo through the Development of a Multiple Humanized Mouse Model
JF  - Drug Metabolism and Disposition
Y1  - 2015
U6  - http://dx.doi.org/10.1124/dmd.115.065656
SN  - 1521-009x
VL  - 43
IS  - 11
SP  - 1679
EP  - 1690
PB  - ASPET
CY  - Bethesda
ER  - 
TY  - JOUR
A1  - Kapelyukh, Yury
A1  - Henderson, Colin James
A1  - Scheer, Nico
A1  - Rode, Anja
A1  - Wolf, Charles Roland
T1  - Defining the contribution of CYP1A1 and CYP1A2 to drug metabolism using humanized CYP1A1/1A2 and Cyp1a1/Cyp1a2 KO mice
JF  - Drug Metabolism and Disposition
Y1  - 2019
U6  - http://dx.doi.org/10.1124/dmd.119.087718
IS  - Early view
ER  - 
TY  - JOUR
A1  - Scheer, Nico
A1  - Mclaughlin, Lesley A.
A1  - Rode, Anja
A1  - MacLeod, Alastair Kenneth
A1  - Henderson, Colin J.
A1  - Wolf, Roland C.
T1  - Deletion of thirty murine cytochrome P450 genes results in viable mice with compromised drug metabolism
JF  - Drug Metabolism and Disposition
N2  - In humans, 75% of all drugs are metabolized by the cytochrome P450-dependent monooxygenase system. Enzymes encoded by the CYP2C, CYP2D, and CYP3A gene clusters account for ∼80% of this activity. There are profound species differences in the multiplicity of cytochrome P450 enzymes, and the use of mouse models to predict pathways of drug metabolism is further complicated by overlapping substrate specificity between enzymes from different gene families. To establish the role of the hepatic and extrahepatic P450 system in drug and foreign chemical disposition, drug efficacy, and toxicity, we created a unique mouse model in which 30 cytochrome P450 genes from the Cyp2c, Cyp2d, and Cyp3a gene clusters have been deleted. Remarkably, despite a wide range of putative important endogenous functions, Cyp2c/2d/3a KO mice were viable and fertile, demonstrating that these genes have evolved primarily as detoxification enzymes. Although there was no overt phenotype, detailed examination showed Cyp2c/2d/3a KO mice had a smaller body size (15%) and larger livers (20%). Changes in hepatic morphology and a decreased blood glucose (30%) were also noted. A five-drug cocktail of cytochrome P450 isozyme probe substrates were used to evaluate changes in drug pharmacokinetics; marked changes were observed in either the pharmacokinetics or metabolites formed from Cyp2c, Cyp2d, and Cyp3a substrates, whereas the metabolism of the Cyp1a substrate caffeine was unchanged. Thus, Cyp2c/2d/3a KO mice provide a powerful model to study the in vivo role of the P450 system in drug metabolism and efficacy, as well as in chemical toxicity.
Y1  - 2014
U6  - http://dx.doi.org/10.1124/dmd.114.057885
SN  - 1521-009X
VL  - 42
IS  - 6
SP  - 1022
EP  - 1030
PB  - ASPET
CY  - Bethesda, Md.
ER  - 
TY  - PAT
A1  - Jeromin, Günter Erich
T1  - Denk- und Lernspielzeug : Offenlegungsschrift : DE 102006003433 A1 ; Offenlegungstag: 26.07.2007
Y1  - 2007
N1  - Volltext über Datenbank: http://publikationen.dpma.de/
PB  - Deutsches Patent- und Markenamt
CY  - München
ER  - 
TY  - JOUR
A1  - Prielmeier, Franz
A1  - Radkowitsch, H.
A1  - Lang, E. W.
A1  - Lüdemann, H.-D.
T1  - Density dependence of the molecular dynamics of fluid CH3F and CF3H studied by NMR / H. Radkowitsch, F. X.  Prielmeier, E. W. Lang and H.-D. Lüdemann
JF  - Physica B+C. 139-140 (1986)
Y1  - 1986
SN  - 0921-4526
SP  - 96
EP  - 99
ER  - 
TY  - JOUR
A1  - Kotter, Michael
A1  - Riekert, L.
A1  - Weyland, F.
T1  - Deposition of ternary oxides as active components by impregnation of porous carriers
JF  - Preparation of Catalysts III : scientific bases for the preparation of heterogeneous catalysts ; proceedings of the Third International Symposium, Louvain-la-Neuve, September 6-9, 1982 / ed.: G. Poncelet ... - (Studies in surface science and catalysis ; 16)
Y1  - 1983
SN  - 0-444-42184-X
N1  - International Symposium on the Scientific Bases for the Preparation of Heterogeneous Catalysts <3, 1982, Louvain-la-Neuve>
SP  - 521
EP  - 530
PB  - Elsevier
CY  - Amsterdam [u.a.]
ER  - 
TY  - JOUR
A1  - Hoffstadt, Kevin
A1  - Cheenakula, Dheeraja
A1  - Nikolausz, Marcell
A1  - Krafft, Simone
A1  - Harms, Hauke
A1  - Kuperjans, Isabel
T1  - Design and construction of a new reactor for flexible biomethanation of hydrogen
JF  - Fermentation
N2  - The increasing share of renewable electricity in the grid drives the need for sufficient storage capacity. Especially for seasonal storage, power-to-gas can be a promising approach. Biologically produced methane from hydrogen produced from surplus electricity can be used to substitute natural gas in the existing infrastructure. Current reactor types are not or are poorly optimized for flexible methanation. Therefore, this work proposes a new reactor type with a plug flow reactor (PFR) design. Simulations in COMSOL Multiphysics ® showed promising properties for operation in laminar flow. An experiment was conducted to support the simulation results and to determine the gas fraction of the novel reactor, which was measured to be 29%. Based on these simulations and experimental results, the reactor was constructed as a 14 m long, 50 mm diameter tube with a meandering orientation. Data processing was established, and a step experiment was performed. In addition, a kLa of 1 h−1 was determined. The results revealed that the experimental outcomes of the type of flow and gas fractions are in line with the theoretical simulation. The new design shows promising properties for flexible methanation and will be tested.
KW  - methanation
KW  - plug flow reactor
KW  - bubble column
KW  - bio-methane
KW  - power-to-gas
Y1  - 2023
U6  - http://dx.doi.org/10.3390/fermentation9080774
SN  - 2311-5637
N1  - The article belongs to the Special Issue Fermentation Processes: Modeling, Optimization and Control
VL  - 9
IS  - 8
SP  - 1
EP  - 16
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Kalbe, Jochen
A1  - Höcker, Hartwig
A1  - Berndt, Heinz
T1  - Design of enzyme reactors as chromatographic columns for racemic resolution of amino acid esters
JF  - Chromatographia
Y1  - 1989
SN  - 0009-5893
U6  - http://dx.doi.org/10.1007/BF02319646
VL  - 28
IS  - 3-4
SP  - 193
EP  - 196
ER  - 
TY  - JOUR
A1  - Leurs, Ulrike
A1  - Mezo, Gabor
A1  - Öhlschläger, Peter
A1  - Orban, Erika
A1  - Marquard, Andrea
A1  - Manea, Marilena
T1  - Design, synthesis, in vitro stability and cytostatic effect of multifunctional anticancer drug-bioconjugates containing GnRH-III as a targeting moiety
JF  - Peptide Science
N2  - Bioconjugates containing the GnRH-III hormone decapeptide as a targeting moiety are able to deliver chemotherapeutic agents specifically to cancer cells expressing GnRH receptors, thereby increasing their local efficacy while limiting the peripheral toxicity. However, the number of GnRH receptors on cancer cells is limited and they desensitize under continuous hormone treatment. A possible approach to increase the receptor mediated tumor targeting and consequently the cytostatic effect of the bioconjugates would be the attachment of more than one chemotherapeutic agent to one GnRH-III molecule. Here we report on the design, synthesis and biochemical characterization of multifunctional bioconjugates containing GnRH-III as a targeting moiety and daunorubicin as a chemotherapeutic agent. Two different drug design approaches were pursued. The first one was based on the bifunctional [4Lys]-GnRH-III (Glp-His-Trp-Lys-His-Asp-Trp-Lys-Pro-Gly-NH2) containing two lysine residues in positions 4 and 8, whose ϵ-amino groups were used for the coupling of daunorubicin. In the second drug design, the native GnRH-III (Glp-His-Trp-Ser-His-Asp-Trp-Lys-Pro-Gly-NH2) was used as a scaffold; an additional lysine residue was coupled to the ϵ-amino group of 8Lys in order to generate two free amino groups available for conjugation of daunorubicin. The in vitro stability/degradation of all synthesized compounds was investigated in human serum, as well as in the presence of rat liver lysosomal homogenate. Their cellular uptake was determined on human breast cancer cells and the cytostatic effect was evaluated on human breast, colon and prostate cancer cell lines. Compared with a monofunctional compound, both drug design approaches resulted in multifunctional bioconjugates with increased cytostatic effect.
Y1  - 2012
U6  - http://dx.doi.org/10.1002/bip.21640
SN  - 1097-0282
VL  - 98
IS  - 1
SP  - 1
EP  - 10
PB  - Wiley
CY  - New York, NY
ER  - 
TY  - JOUR
A1  - Schmich, Peter
A1  - Ederer, Hanns J.
A1  - Ebert, Klaus H.
T1  - Detection and identification of free radicals in hydrocarbon pyrolysis by an iodine trapping method
JF  - Industrial & Engineering Chemistry Research. 31 (1992), H. 1
Y1  - 1992
SN  - 1520-5045
SP  - 29
EP  - 37
ER  - 
TY  - JOUR
A1  - Welden, Melanie
A1  - Severins, Robin
A1  - Poghossian, Arshak
A1  - Wege, Christina
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Detection of acetoin and diacetyl by a tobacco mosaic virus-assisted field-effect biosensor
JF  - Chemosensors
N2  - Acetoin and diacetyl have a major impact on the flavor of alcoholic beverages such as wine or beer. Therefore, their measurement is important during the fermentation process. Until now, gas chromatographic techniques have typically been applied; however, these require expensive laboratory equipment and trained staff, and do not allow for online monitoring. In this work, a capacitive electrolyte–insulator–semiconductor sensor modified with tobacco mosaic virus (TMV) particles as enzyme nanocarriers for the detection of acetoin and diacetyl is presented. The enzyme acetoin reductase from Alkalihalobacillus clausii DSM 8716ᵀ is immobilized via biotin–streptavidin affinity, binding to the surface of the TMV particles. The TMV-assisted biosensor is electrochemically characterized by means of leakage–current, capacitance–voltage, and constant capacitance measurements. In this paper, the novel biosensor is studied regarding its sensitivity and long-term stability in buffer solution. Moreover, the TMV-assisted capacitive field-effect sensor is applied for the detection of diacetyl for the first time. The measurement of acetoin and diacetyl with the same sensor setup is demonstrated. Finally, the successive detection of acetoin and diacetyl in buffer and in diluted beer is studied by tuning the sensitivity of the biosensor using the pH value of the measurement solution.
Y1  - 2022
U6  - http://dx.doi.org/10.3390/chemosensors10060218
SN  - 2227-9040
N1  - This article belongs to the Special Issue "Nanostructured Devices for Biochemical Sensing"
VL  - 10
IS  - 6
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Molinnus, Denise
A1  - Bäcker, Matthias
A1  - Siegert, Petra
A1  - Willenberg, H.
A1  - Poghossian, Arshak
A1  - Keusgen, M.
A1  - Schöning, Michael Josef
T1  - Detection of Adrenaline Based on Substrate Recycling Amplification
JF  - Procedia Engineering
N2  - An amperometric enzyme biosensor has been applied for the detection of adrenaline. The adrenaline biosensor has been prepared by modification of an oxygen electrode with the enzyme laccase that operates at a broad pH range between pH 3.5 to pH 8. The enzyme molecules were immobilized via cross-linking with glutaraldehyde. The sensitivity of the developed adrenaline biosensor in different pH buffer solutions has been studied.
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.proeng.2015.08.708
SN  - 1877-7058
N1  - Eurosensors 2015
VL  - 120
SP  - 540
EP  - 543
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Takenaga, Shoko
A1  - Herrera, Cony F.
A1  - Werner, Frederik
A1  - Biselli, Manfred
A1  - Schnitzler, Thomas
A1  - Schöning, Michael Josef
A1  - Öhlschläger, Peter
A1  - Wagner, Torsten
T1  - Detection of the metabolic activity of cells by differential measurements based on a single light-addressable potentiometric sensor chip
T2  - 11. Dresdner Sensor-Symposium : 9.-11.12.2013
Y1  - 2013
SN  - 978-3-9813484-5-3
SP  - 63
EP  - 67
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Deterding, A.
A1  - Ulber, Roland
T1  - Determination of acetic acid in fermentation broth by gas-diffusion technique
JF  - Engineering in Life Sciences
N2  - Due to the interfering effects of acetic acid in many fermentation processes, a gas-diffusion technique was developed for the online determination of acetic acid. The measurements were accomplished with a flow diffusion analysis (FDA) unit from the TRACE Analytics GmbH, Braunschweig, Germany. The diffusion analysis is based on the UV-absorbance of acetic acid at 205 nm. The measurement was achieved by the separation of an acceptor and a carrier stream (acidified fermentation broth) using a gas permeable polytetrafluoroethylene (PTFE) membrane, whereby broth constituents that would otherwise disturb the UV-measurement of acetic acid, are held back efficiently. Merely, the fermentation by-products, e.g. formic acid, is capable of diffusing through the membrane. While formic acid can disturb the measurement, carbon dioxide does not absorb at 205 nm. The method operates with time-dependent sample enrichment. During the analysis, a small volume of the acceptor stream is stopped for a defined time interval in the acceptor chamber. During this period, the gaseous acetic acid diffuses through the membrane and is enriched in the acceptor chamber. Subsequently after the enrichment, the acceptor stream flows through a UV-detector. The intensity of the signal is proportional to the acetic acid concentration. Online measurements in bioreactors via a sterile filtration probe have been accomplished. A linear calibration in the range of 0.5–5.0 g/L acetic acid with a relative standard deviation of <5 % was obtained. A sampling rate of 8 samples per hour was possible. The system was applied for the determination of acetic acid in E. coli fermentation broth. The instrument is easy to clean, very user-friendly and does not require any toxic or expensive reagents.
Y1  - 2008
U6  - http://dx.doi.org/10.1002/elsc.200820227
VL  - 8
IS  - 1, Special Issue: Technical Systems for the Use in Life Sciences
SP  - 62
EP  - 67
ER  - 
TY  - JOUR
A1  - Werner, Frederik
A1  - Krumbe, Christoph
A1  - Schumacher, Katharina
A1  - Groebel, Simone
A1  - Spelthahn, Heiko
A1  - Stellberg, Michael
A1  - Wagner, Torsten
A1  - Yoshinobu, Tatsuo
A1  - Selmer, Thorsten
A1  - Keusgen, Michael
A1  - Baumann, Marcus
A1  - Schöning, Michael Josef
T1  - Determination of the extracellular acidification of Escherichia coli by a light-addressable potentiometric sensor
JF  - Physica status solidi (a) : applications and material science. 208 (2011), H. 6
Y1  - 2011
SN  - 1862-6319
SP  - 1340
EP  - 1344
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Türler, A.
A1  - Gäggeler, H. W.
A1  - Jost, D. T.
T1  - Determination of the Partial Electron-Capture- and Spontaneous-Fission Half-Lives of 254No / A. Türler, H.W. Gäggeler, D.T. Jost, P. Armbruster, W. Brüchle, H. Folger, F.P. Heßberger,  S. Hofmann,
JF  - Zeitschrift für Physik A Hadrons and Nuclei. 331 (1988), H. 3
Y1  - 1988
SN  - 0939-7922
SP  - 363
EP  - 364
ER  - 
TY  - JOUR
A1  - Küppers, Tobias
A1  - Steffen, Victoria
A1  - Hellmuth, Hendrik
A1  - O'Connell, Timothy
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - Wiechert, Wolfgang
T1  - Developing a new production host from a blueprint: Bacillus pumilus as an industrial enzyme producer
JF  - Microbial cell factories
Y1  - 2014
U6  - http://dx.doi.org/10.1186/1475-2859-13-46
SN  - 1475-2859 (E-Journal)
VL  - 13
SP  - Article No. 46
PB  - BioMed Central
CY  - London
ER  -