TY - JOUR A1 - Biselli, Manfred A1 - Jelinek, N. A1 - Schmidt, S. A1 - Hilbert, U. T1 - Neue Bioreaktoren zur Kultivierung hämatopoetischer Zellen / Jelinek, N. ; Schmidt, S. ; Hilbert, U. ; Thoma, S. ; Biselli, M. ; Wandrey, C. JF - Chemie Ingenieur Technik. 73 (2001), H. 7 Y1 - 2001 SN - 0009-286X SP - 894 EP - 898 ER - TY - BOOK A1 - Berndt, Heinz T1 - Neue Kondensations-Methoden zur Synthese definierter Peptid-Derivate Y1 - 2014 N1 - Aachen, Techn. Hochsch., Habil.-Schr., 1982 CY - Aachen ER - TY - PAT A1 - Siegert, Petra A1 - Merkel, Marion A1 - Kluin, Cornelia A1 - Maurer, Karl-Heinz A1 - O'Connell, Timothy A1 - Wieland, Susanne A1 - Hellmuth, Hendrik T1 - Neue Proteasen und diese enthaltende Mittel [Offenlegungsschrift] T1 - Novel proteases and compositions comprising them [Europäische Patentanmeldung / Internationale Patentanmeldung] Y1 - 2011 SP - 1 EP - 21 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO CY - München / Den Hague / Genf ER - TY - PAT A1 - Siegert, Petra A1 - Mussmann, Nina A1 - O'Connell, Timothy A1 - Maurer, Karl-Heinz T1 - Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift] T1 - Novel proteases and means containing said proteases [Internationale Patentanmeldung] Y1 - 2010 SP - 1 EP - 30 PB - Deutsches Patent- und Markenamt / WIPO CY - München / Genf ER - TY - PAT A1 - Siegert, Petra A1 - Baumstark, Rebecca A1 - Kluin, Cornelia A1 - O'Connell, Timothy A1 - Maurer, Karl-Heinz A1 - Hellmuth, Hendrik T1 - Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift] Y1 - 2010 SP - 1 EP - 30 PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Siegert, Petra A1 - Spitz, Astrid A1 - Maurer, Karl-Heinz T1 - Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift] T1 - Novel proteases and compositions comprising these proteases [Internationale Patentanmeldung] Y1 - 2010 SP - 1 EP - 31 PB - Deutsches Patentamt / WIPO CY - München / Genf ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Mang, Thomas T1 - Neue Technik mit magnetisierbaren Materialien : Offenlegungsschrift DE102004005201A1 ; Offenlegungstag: 11.08.2005 Y1 - 2005 N1 - Volltext über Datenbank DEPATISnet PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Schwarzer, Klemens T1 - Neuer Wärmeträger für Solaranlagen : Offenlegungsschrift : DE 102007016738 A1 ; Offenlegungstag: 09.10.2008 Y1 - 2008 N1 - Volltext über Datenbank DEPATISnet: http://depatisnet.dpma.de/DepatisNet/depatisnet?action=pdf&docid=DE102007016738A1 PB - Deutsches Patent- und Markenamt CY - München ER - TY - JOUR A1 - Selmer, Thorsten A1 - Pierik, Antonio J. A1 - Heider, Johann T1 - New glycyl radical enzymes catalysing key metabolic steps in anaerobic bacteria JF - Biological Chemistry. 386 (2005), H. 10 Y1 - 2005 SN - 1431-6730 SP - 981 EP - 988 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Kratz, J. V. A1 - Gober, M. K. A1 - Zimmermann, H. P. T1 - New nuclide 263 105 / J.V. Kratz, M.K. Gober, H.P. Zimmermann, M. Schädel, W. Brüchle, E. Schimpf, K.E. Gregorich, A. Türler, N.J. Hannink, K.R. Czerwinski, B. Kadkhodayan, D.M. Lee, M.J. Nurmia, D.C. Hoffman, H. Gäggeler, D. Jost, U.W. Scherer, A. Weber JF - Physical Review C . 45 (1992) Y1 - 1992 SP - 1064 EP - 1069 ER - TY - JOUR A1 - Falkenberg, Fabian A1 - Voß, Leonie A1 - Bott, Michael A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - New robust subtilisins from halotolerant and halophilic Bacillaceae JF - Applied Microbiology and Biotechnology N2 - The aim of the present study was the characterisation of three true subtilisins and one phylogenetically intermediate subtilisin from halotolerant and halophilic microorganisms. Considering the currently growing enzyme market for efficient and novel biocatalysts, data mining is a promising source for novel, as yet uncharacterised enzymes, especially from halophilic or halotolerant Bacillaceae, which offer great potential to meet industrial needs. Both halophilic bacteria Pontibacillus marinus DSM 16465ᵀ and Alkalibacillus haloalkaliphilus DSM 5271ᵀ and both halotolerant bacteria Metabacillus indicus DSM 16189 and Litchfieldia alkalitelluris DSM 16976ᵀ served as a source for the four new subtilisins SPPM, SPAH, SPMI and SPLA. The protease genes were cloned and expressed in Bacillus subtilis DB104. Purification to apparent homogeneity was achieved by ethanol precipitation, desalting and ion-exchange chromatography. Enzyme activity could be observed between pH 5.0–12.0 with an optimum for SPPM, SPMI and SPLA around pH 9.0 and for SPAH at pH 10.0. The optimal temperature for SPMI and SPLA was 70 °C and for SPPM and SPAH 55 °C and 50 °C, respectively. All proteases showed high stability towards 5% (w/v) SDS and were active even at NaCl concentrations of 5 M. The four proteases demonstrate potential for future biotechnological applications. KW - Biotechnological application KW - Bacillaceae KW - Subtilisin KW - Subtilases KW - Halotolerant protease Y1 - 2023 U6 - http://dx.doi.org/10.1007/s00253-023-12553-w SN - 1432-0614 N1 - Corresponding author: Petra Siegert VL - 107 SP - 3939 EP - 3954 PB - Springer Nature CY - Berlin ER - TY - JOUR A1 - Thiel, Alexander A1 - Tippkötter, Nils A1 - Suck, Kirstin A1 - Sohling, Ulrich A1 - Ruf, Friedrich A1 - Ulber, Roland T1 - New zeolite adsorbents for downstream processing of polyphenols from renewable resources JF - Engineering in Life Sciences N2 - Commercial materials with polyvinylpolypyrrolidone and polymeric amberlites (XAD7HP, XAD16) are commonly used for the adsorptive downstream processing of polyphenols from renewable resources. In this study, beta-zeolite-based adsorbent systems were examined, and their properties were compared to organic resins. Batch adsorption experiments were conducted with synthetic solutions of major polyphenols. Adsorption isotherms and desorption characteristics of individual adsorbent were determined based on these results. Maximum adsorption capacities were calculated using the Langmuir model. For example, the zeolites had capacities up to 203.2 mg/g for ferulic acid. To extend these results to a complex system, additional experiments were performed on rapeseed meal and wheat seed extracts as representative renewable resources. HPLC analysis showed that with 7.5% w/v, which is regarded as the optimum amount of zeolites, zeolites A and B could bind 100% of the major polyphenols as well as release polyphenols at high yields. Additionally, regeneration experiments were performed with isopropyl alcohol at 99°C to evaluate how zeolites regenerate under mild conditions. The results showed only a negligible loss of adsorption capacity and no loss of desorption capacity. In summary, it was concluded that beta-zeolites were promising adsorbents for developing new processes to isolate polyphenols from renewable resources. Y1 - 2013 U6 - http://dx.doi.org/10.1002/elsc.201200188 VL - 13 IS - 3 SP - 239 EP - 246 PB - Wiley CY - Weinheim ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Seubert, Bernhard A1 - Beilharz, Helmut A1 - Fickert, Werner [u.a.] T1 - Niedermolekulare Alkalihuminate : Verfahren zu ihrer Herstellung und Verwendung ; Offenlegungsschrift DE3707909A1 ; Offenlegungstag: 22.09.1988 / Anmelder: Rütgerswerke AG, 6000 Frankfurt. Erfinder: Bernhard Seubert ; Helmut Beilharz ; Werner Fickert ; Günter Jeromin ... Y1 - 1988 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Seubert, Bernhard A1 - Beilharz, Helmut A1 - Fickert, Werner [u.a.] T1 - Niedermolekulare Alkalihuminate : Verfahren zur ihrer Herstellung und Verwendung ; Europäische Patentschrift EP0281679B1 ; Veröffentlichungstag im Patentblatt: 01.08.1991 / Anmelder: Rütgerswerke AG, 6000 Frankfurt. Erfinder: Bernhard Seubert ; Helmut Beilharz ; Günter Jeromin ; Werner Fickert ... Y1 - 1991 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - JOUR A1 - Elbers, Gereon A1 - Gäb, Martina A1 - Hoffmann, Katharina A1 - Lobe, Melanie T1 - NIR-spectroscopic investigation of foliage of ozone-stressed Fagus sylvatica trees / Gäb, Martina ; Hoffmann, Katharina ; Lobe, Melanie ; Metzger, Rut ; Ooyen, Sven van ; Elbers, Gereon ; Köllner, Barbara JF - Journal of Forest Research. 11 (2006), H. 2 Y1 - 2006 SN - 1341-6979 SP - 69 EP - 75 ER - TY - JOUR A1 - Tippkötter, Nils A1 - Roikaew, W. A1 - Ulber, R. T1 - Nitrate removal from whey concentrate with biotechnological regeneration of the waste water JF - European dairy magazine : EDM Y1 - 2008 SN - 0936-6318 IS - 1 SP - 30 EP - 32 ER - TY - JOUR A1 - Tippkötter, Nils A1 - Roikaew, N. A1 - Ulber, R. T1 - Nitratentfernung aus Molkekonzentrat mit biotechnologischer Regeneration der Abwässer JF - Deutsche Milchwirtschaft Y1 - 2007 SN - 0012-0480 VL - 58 IS - 15 SP - 540 EP - 542 ER - TY - JOUR A1 - Ulber, Roland A1 - Tippkötter, Nils T1 - Nitratfreie Molke JF - Rundschau für Fleischhygiene und Lebensmittelüberwachung Y1 - 2009 IS - 4 SP - 150 EP - 152 ER - TY - JOUR A1 - Braband, Henrik A1 - Paulßen, Elisabeth A1 - Abram, Ulrich T1 - Nitridorhenium(V) Complexes with 1,3-Dialkyl-4,5-dimethylimidazole-2-ylidenes JF - Zeitschrift für anorganische und allgemeine Chemie : ZAAC = Journal of inorganic and general chemistry Y1 - 2006 U6 - http://dx.doi.org/10.1002/zaac.200600002 SN - 1521-3749 VL - 632 IS - 6 SP - 1051 EP - 1056 ER - TY - JOUR A1 - Baumann, Marcus A1 - Semeneh, M. A1 - Dehairs, F. A1 - Elskens, M. T1 - Nitrogen uptake regime and phytoplankton community structure in the Southern Ocean / Semeneh, M. ; Dehairs, F. ; Elskens, M. ; Baumann, M.E.M. ; Kopczynska, E.E. ; Lancelot, C. ; Goeyens, L. JF - Journal of Marine Systems. 17 (1998), H. 1-4 Y1 - 1998 SN - 0924-7963 SP - 159 EP - 177 ER - TY - JOUR A1 - Kotter, Michael A1 - Greger, M. A1 - Ihme, B. T1 - Nonequilibrium phase transitions with hysteresis in solid catalysts : the system copper-oxygen-propene / M. Greger ; B. Ihme ; M. Kotter ... JF - Berichte der Bunsen-Gesellschaft für Physikalische Chemie. 88 (1984) Y1 - 1984 SN - 0005-9021 SP - 427 EP - 433 ER - TY - JOUR A1 - Lawson, Nathan D. A1 - Scheer, Nico A1 - Pham, Van N. A1 - Kim, Ceol-Hee A1 - Chitnis, Ajay B. A1 - Campos-Ortega, José A. A1 - Weinstein, Brant M. T1 - Notch signaling is required for arterial-venous differentiation during embryonic vascular development JF - Development Y1 - 2001 SN - 1477-9129 VL - 128 IS - 19 SP - 3675 EP - 3683 ER - TY - JOUR A1 - Haeger, Gerrit A1 - Probst, Johanna A1 - Jaeger, Karl-Erich A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - Novel aminoacylases from Streptomyces griseus DSM 40236 and their recombinant production in Streptomyces lividans JF - FEBS Open Bio N2 - Amino acid-based surfactants are valuable compounds for cosmetic formulations. The chemical synthesis of acyl-amino acids is conventionally performed by the Schotten-Baumann reaction using fatty acyl chlorides, but aminoacylases have also been investigated for use in biocatalytic synthesis with free fatty acids. Aminoacylases and their properties are diverse; they belong to different peptidase families and show differences in substrate specificity and biocatalytic potential. Bacterial aminoacylases capable of synthesis have been isolated from Burkholderia, Mycolicibacterium, and Streptomyces. Although several proteases and peptidases from S. griseus have been described, no aminoacylases from this species have been identified yet. In this study, we investigated two novel enzymes produced by S. griseus DSM 40236ᵀ . We identified and cloned the respective genes and recombinantly expressed an α-aminoacylase (EC 3.5.1.14), designated SgAA, and an ε-lysine acylase (EC 3.5.1.17), designated SgELA, in S. lividans TK23. The purified aminoacylase SgAA was biochemically characterized, focusing on its hydrolytic activity to determine temperature- and pH optima and stabilities. The aminoacylase could hydrolyze various acetyl-amino acids at the Nα -position with a broad specificity regarding the sidechain. Substrates with longer acyl chains, like lauroyl-amino acids, were hydrolyzed to a lesser extent. Purified aminoacylase SgELA specific for the hydrolysis of Nε -acetyl-L-lysine was unstable and lost its enzymatic activity upon storage for a longer period but could initially be characterized. The pH optimum of SgELA was pH 8.0. While synthesis of acyl-amino acids was not observed with SgELA, SgAA catalyzed the synthesis of lauroyl-methionine. KW - Streptomyces lividans KW - recombinant expression KW - Streptomyces griseus KW - ε-lysine acylase KW - α-aminoacylase Y1 - 2023 U6 - http://dx.doi.org/10.1002/2211-5463.13723 SN - 2211-5463 N1 - Corresponding author: Petra Siegert VL - 13 IS - 12 SP - 2224 EP - 2238 PB - Wiley CY - Hoboken, NJ ER - TY - JOUR A1 - Monakhova, Yulia A1 - Diehl, Bernd W.K. T1 - Novel approach of qNMR workflow by standardization using 2H integral: Application to any intrinsic calibration standard JF - Talanta N2 - Quantitative nuclear magnetic resonance (qNMR) is routinely performed by the internal or external standardization. The manuscript describes a simple alternative to these common workflows by using NMR signal of another active nuclei of calibration compound. For example, for any arbitrary compound quantification by NMR can be based on the use of an indirect concentration referencing that relies on a solvent having both 1H and 2H signals. To perform high-quality quantification, the deuteration level of the utilized deuterated solvent has to be estimated. In this contribution the new method was applied to the determination of deuteration levels in different deuterated solvents (MeOD, ACN, CDCl3, acetone, benzene, DMSO-d6). Isopropanol-d6, which contains a defined number of deuterons and protons, was used for standardization. Validation characteristics (precision, accuracy, robustness) were calculated and the results showed that the method can be used in routine practice. Uncertainty budget was also evaluated. In general, this novel approach, using standardization by 2H integral, benefits from reduced sample preparation steps and uncertainties, and can be applied in different application areas (purity determination, forensics, pharmaceutical analysis, etc.). KW - qNMR KW - Deuterium NMR KW - Deuterated solvents KW - Standardization Y1 - 2021 SN - 0039-9140 U6 - http://dx.doi.org/10.1016/j.talanta.2020.121504 VL - 222 IS - Article number: 121504 PB - Elsevier ER - TY - JOUR A1 - Biselli, Manfred A1 - Jelinek, N. A1 - Schmidt, S. A1 - Hilbert, U. T1 - Novel bioreactors for the ex vivo cultivation of hematopoietic cells / Jelinek, N. ; Schmidt, S. ; Hilbert, U. ; Thoma, S. ; Biselli, M. ; Wandrey, C. JF - Engineering in Life Sciences. 2 (2002), H. 1 Y1 - 2002 SN - 1618-0240 SP - 15 EP - 18 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Jennemann, Richard A1 - Bauer, Bernhard L. A1 - Bertalanffy, Helmut T1 - Novel glycoinositolphosphosphingolipids, basidiolipids, from Agaricus / Jennemann, Richard ; Bauer, Bernhard, L. ; Bertalanffy, Helmut ; Geyer, Rudolf ; Gschwind, Ruth, M. ; Selmer, Thorsten ; Wiegandt, Herbert JF - European Journal of Biochemistry. 259 (1999), H. 1-2 Y1 - 1999 SN - 0014-2956 SP - 331 EP - 338 ER - TY - JOUR A1 - Monakhova, Yulia A1 - Diehl, Bernd W.K. T1 - Nuclear magnetic resonance spectroscopy as an elegant tool for a complete quality control of crude heparin material JF - Journal of Pharmaceutical and Biomedical Analysis N2 - Nuclear magnetic resonance (NMR) spectrometric methods for the quantitative analysis of pure heparin in crude heparin is proposed. For quantification, a two-step routine was developed using a USP heparin reference sample for calibration and benzoic acid as an internal standard. The method was successfully validated for its accuracy, reproducibility, and precision. The methodology was used to analyze 20 authentic porcine heparinoid samples having heparin content between 4.25 w/w % and 64.4 w/w %. The characterization of crude heparin products was further extended to a simultaneous analysis of these common ions: sodium, calcium, acetate and chloride. A significant, linear dependence was found between anticoagulant activity and assayed heparin content for thirteen heparinoids samples, for which reference data were available. A Diffused-ordered NMR experiment (DOSY) can be used for qualitative analysis of specific glycosaminoglycans (GAGs) in heparinoid matrices and, potentially, for quantitative prediction of molecular weight of GAGs. NMR spectrometry therefore represents a unique analytical method suitable for the simultaneous quantitative control of organic and inorganic composition of crude heparin samples (especially heparin content) as well as an estimation of other physical and quality parameters (molecular weight, animal origin and activity). KW - NMR spectroscopy KW - Heparin KW - Crude heparin KW - USP KW - Ions Y1 - 2022 U6 - http://dx.doi.org/10.1016/j.jpba.2022.114915 SN - 0731-7085 VL - 219 IS - Article number: 114915 PB - Elsevier CY - New York, NY ER - TY - JOUR A1 - Stanley, Lesley A. A1 - Horsburgh, Brian C. A1 - Ross, Jillian A1 - Scheer, Nico A1 - Wolf, C. Roland T1 - Nuclear Receptors which play a pivotal role in drug disposition and chemical toxicity JF - Drug Metabolism Reviews Y1 - 2006 U6 - http://dx.doi.org/10.1080/03602530600786232 SN - 1097-9883 VL - 38 IS - 3 SP - 515 EP - 597 ER - TY - JOUR A1 - Werner, Frederik A1 - Groebel, Simone A1 - Krumbe, Christoph A1 - Wagner, Torsten A1 - Selmer, Thorsten A1 - Yoshinobu, Tatsuo A1 - Baumann, Marcus A1 - Schöning, Michael Josef T1 - Nutrient concentration-sensitive microorganism-based biosensor JF - Physica Status Solidi (a) Y1 - 2012 U6 - http://dx.doi.org/10.1002/pssa.201100801 SN - 1862-6319 VL - 209 IS - 5 SP - 900 EP - 904 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Unden, G. A1 - Becker, S. A1 - Bongaerts, Johannes A1 - Holighaus, G. A1 - Schirawski, J. A1 - Six, S. T1 - O2-sensing and O2-dependent gene regulation in facultatively anaerobic bacteria JF - Archives of microbiology Y1 - 1995 SN - 1432-072X (E-Journal); 0003-9276 (Print); 0302-8933 (Print) VL - Vol. 164 IS - Iss. 2 SP - 81 EP - 90 ER - TY - JOUR A1 - Kornfeld, Jan-Wilhelm A1 - Baitzel, Catherina A1 - Könner, A. Christine A1 - Nicholls, Hayley T. A1 - Vogt, Merly C. A1 - Herrmanns, Karolin A1 - Scheja, Ludger A1 - Haumaitre, Cécile A1 - Wolf, Anna M. A1 - Knippschild, Uwe A1 - Seibler, Jost A1 - Cereghini, Silvia A1 - Heeren, Joerg A1 - Stoffel, Markus A1 - Brüning, Jens C. T1 - Obesity-induced overexpression of miR-802 impairs glucose metabolism through silencing of Hnf1b JF - Nature Y1 - 2013 SN - 0028-0836 U6 - http://dx.doi.org/10.1038/nature11793 VL - 494 IS - 7435 SP - 111 EP - 115 PB - Springer Nature CY - Cham ER - TY - JOUR A1 - Jordan, Sabine D. A1 - Krüger, Markus A1 - Willmes, Diana M. A1 - Redemann, Nora A1 - Wunderlich, F. Thomas A1 - Brönneke, Hella S. A1 - Merkwirth, Carsten A1 - Kashkar, Hamid A1 - Olkkonen, Vesa M. A1 - Böttger, Thomas A1 - Braun, Thomas A1 - Seibler, Jost A1 - Brüning, Jens C. T1 - Obesity-induced overexpression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism JF - Nature Cell Biology N2 - The contribution of altered post-transcriptional gene silencing to the development of insulin resistance and type 2 diabetes mellitus so far remains elusive. Here, we demonstrate that expression of microRNA (miR)-143 and 145 is upregulated in the liver of genetic and dietary mouse models of obesity. Induced transgenic overexpression of miR-143, but not miR-145, impairs insulin-stimulated AKT activation and glucose homeostasis. Conversely, mice deficient for the miR-143–145 cluster are protected from the development of obesity-associated insulin resistance. Quantitative-mass-spectrometry-based analysis of hepatic protein expression in miR-143-overexpressing mice revealed miR-143-dependent downregulation of oxysterol-binding-protein-related protein (ORP) 8. Reduced ORP8 expression in cultured liver cells impairs the ability of insulin to induce AKT activation, revealing an ORP8-dependent mechanism of AKT regulation. Our experiments provide direct evidence that dysregulated post-transcriptional gene silencing contributes to the development of obesity-induced insulin resistance, and characterize the miR-143–ORP8 pathway as a potential target for the treatment of obesity-associated diabetes. Y1 - 2011 U6 - http://dx.doi.org/10.1038/ncb2211 SN - 1465-7392 VL - 13 IS - 4 SP - 434 EP - 446 PB - Nature CY - New York ER - TY - JOUR A1 - Ratke, Lorenz A1 - Milow, Barbara A1 - Lisinski, Susanne A1 - Hoepfner, Sandra T1 - On an effect of fine ceramic particles on the structure of aerogels JF - Microgravity science and technology Y1 - 2014 U6 - http://dx.doi.org/10.1007/s12217-014-9380-2 SN - 0938-0108 ; 1875-0494 VL - 26 SP - 103 EP - 110 PB - Springer Nature CY - Heidelberg ER - TY - JOUR A1 - Biselli, Manfred A1 - Noll, T. A1 - Wandrey, C. T1 - On-line biomass monitoring of immobilized hybridoma cells by dielectrical measurements / Noll, T. ; Biselli, M. ; Wandrey, C. JF - Animal cell technology : from vaccines to genetic medicine ; [Vilamoura, Portugal, May 1996] / ed. by Manuel J. T. Carrondo Y1 - 1997 SN - 0-7923-4321-2 SP - 289 EP - 294 PB - Kluwer CY - Dordrecht ER - TY - JOUR A1 - Biselli, Manfred A1 - Van der Pol, Jens J. A1 - Joksch, Burkhard A1 - Galgens, Jochem T1 - On-line control of an immobilized hybridoma culture with multi-channel flow injektion analysis / Jens J. van der Pol, Burkhard Joksch, Jochem Galgens, Manfred Biselli, Cornelis D. de Gooijer, Johannes Tramper and Christian Wandrey JF - Journal of Biotechnology. 43 (1995), H. 3 Y1 - 1995 SN - 0168-1656 N1 - 3. Autor vermutlich: Jochen Gätgens SP - 229 EP - 242 ER - TY - JOUR A1 - Biselli, Manfred A1 - Van der Pol, Jens J. A1 - Machnik, Marc A1 - Portela-Klein, Theresa T1 - On-line immunoanalysis of monoclonal antibodies during a continuous culture of hybridoma cells / Jens J. van der Pol, Marc Machnik, Manfred Biselli, Theresa Portela-Klein, Cornelis D. de Gooijer, Johannes Tramper und Christian Wandrey JF - Cytotechnology. 24 (1997), H. 1 Y1 - 1997 SN - 0920-9069 SP - 19 EP - 30 ER - TY - JOUR A1 - Biselli, Manfred A1 - Van der Pol, Jens J. A1 - Joksch, B. A1 - Spohn, U. T1 - On-line monitoring and control of glucose, glutamine, lactate and ammonium during a high-cell-density cultivation of hybridoma cells / van der Pol, Jens J. ; Joksch, B. ; Spohn, U. ; Biselli, M. ; Wandrey, C. JF - Proceedings of the sixth international meeting of the Japanese Association for Animal Cell Technology [JAACT 93]: Nagoya, Japan, November 9 - 12, 1993 / ed. by T. Kobayashi Y1 - 1994 SN - 0-7923-3156-7 N1 - International meeting of the Japanese Association for Animal Cell Technology, JAACT 93 ; (6 : ; 1993.11.09-12 : ; Nagoya, Japan) ; Animal cell technology, basic and applied aspects ; 6 SP - 167 EP - 170 PB - Springer Netherland CY - Berlin ER - TY - JOUR A1 - Biselli, Manfred A1 - Van der Pol, Jens J. A1 - Spohn, Uwe A1 - Eberhardt, Rolf T1 - On-line monitoring of an animal cell culture with multi-channel flow injection analysis / van der Pol, Jens J.; Spohn, Uwe ; Eberhardt, Rolf ; Gätgens, Jochen ; Biselli, Manfred ; Wandrey, Christian ; Tramper, Johannes JF - Journal of Biotechnology. 37 (1994), H. 3 Y1 - 1994 SN - 0168-1656 SP - 253 EP - 264 ER - TY - CHAP A1 - Deterding, A. A1 - Tippkötter, Nils A1 - Ulber, R. ED - Beckmann, Dieter T1 - Online-Essigsäureanalytik in Fermentationsbrühen mittels Fließdiffusionstechnik (FDT) T2 - Technische Systeme für Biotechnologie und Umwelt : 13. Heiligenstädter Kolloquium, Heilbad Heiligenstadt, 25.09. - 27.09.2006 Y1 - 2006 SN - 978-3-00-018621-9 SP - 273 EP - 280 CY - Heiligenstadt ER - TY - JOUR A1 - Biselli, Manfred A1 - Machnik, M. A1 - Wandrey, C. T1 - Online-Immunoanalytik bei der kontinuierlichen Fermentation von Hybridomazellen / Machnik, M. ; Biselli, M. ; Wandrey, C. JF - BIO WORLD. 1 (1996), H. 3 Y1 - 1996 SN - 1424-8514 SP - 12 EP - 15 ER - TY - JOUR A1 - Wagner, Torsten A1 - Beging, Stefan A1 - Rotter, L. A1 - Poghossian, Arshak A1 - Biselli, Manfred A1 - Zang, Werner A1 - Schöning, Michael Josef T1 - Online-Messsysteme für die automatisierte Charakterisierung von feldeffektbasierten Biosensoren JF - 8. Dresdner Sensor-Symposium : Sensoren für Umwelt, Klima und Sicherheit, Biosensoren und Biosysteme, Sensoren und Sensorsysteme für die Prozesstechnik, Trends in der Sensortechnik, Materialentwicklung für die Sensorik; 8. Dresdner Sensor-Symposium, 10. - 12. Dezember 2007, Dresden / Gerald Gerlach ... (Hg.) Y1 - 2007 SN - 978-3-940046-45-1 N1 - Dresdner Sensor-Symposium <8, 2007, Dresden> ; Dresdner Beiträge zur Sensorik ; 29 SP - 257 EP - 260 PB - TUDpress, Verl. der Wissenschaften CY - Dresden ER - TY - JOUR A1 - Hough, Lindsay B. A1 - Nalwalk, Julia W. A1 - Ding, Xinxin A1 - Scheer, Nico T1 - Opioid Analgesia in P450 Gene Cluster Knockout Mice: A Search for Analgesia-Relevant Isoforms JF - Drug Metabolism and Disposition Y1 - 2015 U6 - http://dx.doi.org/10.1124/dmd.115.065490 SN - 1521-009x VL - 43 IS - 9 SP - 1326 EP - 1330 ER - TY - JOUR A1 - Niedermeier, Jana A1 - Penner, Crystal A1 - Usherovich, Samuel A1 - Bélanger-Champagne, Camille A1 - Paulßen, Elisabeth A1 - Cornelia, Hoehr T1 - Optical Fibers as Dosimeter Detectors for Mixed Proton/Neutron Fields - A Biological Dosimeter JF - electronics N2 - In recent years, proton therapy has gained importance as a cancer treatment modality due to its conformality with the tumor and the sparing of healthy tissue. However, in the interaction of the protons with the beam line elements and patient tissues, potentially harmful secondary neutrons are always generated. To ensure that this neutron dose is as low as possible, treatment plans could be created to also account for and minimize the neutron dose. To monitor such a treatment plan, a compact, easy to use, and inexpensive dosimeter must be developed that not only measures the physical dose, but which can also distinguish between proton and neutron contributions. To that end, plastic optical fibers with scintillation materials (Gd₂O₂S:Tb, Gd₂O₂S:Eu, and YVO₄:Eu) were irradiated with protons and neutrons. It was confirmed that sensors with different scintillation materials have different sensitivities to protons and neutrons. A combination of these three scintillators can be used to build a detector array to create a biological dosimeter. KW - biological dosimeter KW - Bragg peak KW - relative dosimetry KW - optical fibers KW - proton therapy KW - protons KW - neutrons Y1 - 2023 U6 - http://dx.doi.org/10.3390/electronics12020324 SN - 2079-9292 N1 - This article belongs to the Special Issue "Applications of Optical Fiber Sensors" VL - 12 IS - 2 PB - MDPI CY - Basel ER - TY - JOUR A1 - Srivastava, A. A1 - Singh, V. A1 - Aggarwal, P. A1 - Schneeweiss, F. A1 - Scherer, Ulrich W. A1 - Friedrich, W. T1 - Optical studies of insulating polymers for radiation dose monitoring JF - Indian Journal of Pure & Applied Physics Y1 - 2010 SN - 0019-5596 N1 - Special Issue: SI VL - 48 IS - 11 SP - 782 EP - 786 ER - TY - JOUR A1 - Aggarwal, P. A1 - Dhiman, S. A1 - Kumar, G. A1 - Scherer, Ulrich W. A1 - Singla, M. L. A1 - Srivastava, A. T1 - Optical study of poly(ethyleneterephthalate) modified by different ionizing radiation dose JF - Indian Journal of Pure and Applied Physics N2 - Thin films of poly(ethyleneterephthalate) [PET]were exposed to radiation dose ranging from 10 to 30 kGy by using gamma rays in the range 12.8-177.8 MGy using swift light ions of hydrogen. There was no effect of the radiation dose on the optical behaviour of PET as a result of exposure to radiation dose up to 30 kGy brought about by gamma rays but a significant decrease in the optical band gap values was observed when PET was exposed to swift light ions of hydrogen. The data obtained are discussed in terms of optical studies carried out on PET using swift heavy ions. Y1 - 2012 SN - 0019-5596 VL - 50 IS - 2 SP - 129 EP - 132 ER - TY - JOUR A1 - Jeromin, Günter Erich A1 - Albertz, Michael T1 - Optically active α-acetoxycarboxylic acids and α-hydroxycarboxylic acids by enzyme-aided syntheses JF - Journal für Praktische Chemie / Chemiker-Zeitung. 334 (1992), H. 6 Y1 - 1992 SN - 1436-9966 N1 - ISSN der E-Ausg.: 1521-3897 ; In der Bibliothek (Jülich) vorhanden unter 63 ZS 024-1992 SP - 526 EP - 528 ER - TY - BOOK A1 - Kotter, Michael A1 - Lintz, Hans-Günther T1 - Optimierung der Katalysatorzusammensetzung im Fall der selektiven Reduktion von Stickoxiden durch Ammoniak bei instationärer Reaktionsführung in einem weiten Temperaturbereich. - (Forschungsbericht KfK-PEF ; 33) Y1 - 1987 N1 - ISSN der Serie: 0931-2749 PB - Kernforschungszentrum CY - Karlsruhe ER - TY - CHAP A1 - Tippkötter, Nils A1 - Stückmann, H. A1 - Winkelmann, G. A1 - Noack, U. A1 - Beutel, S. A1 - Scheper, T. A1 - Ulber, R. T1 - Optimisation of antibody-labelling of gold colloids for their application in an immunchromatographic assay for microcystin-LR T2 - European BioPerspectives : celebrating the 25th DECHEMA annual convention of biotechnologists ; 30 May - 1 June 2007, Cologne, Germany ; book of abstracts ; abstracts, poster programme Y1 - 2007 SP - 126 PB - Dechema CY - Frankfurt am Main ER - TY - JOUR A1 - Pilas, Johanna A1 - Yazici, Yasemen A1 - Selmer, Thorsten A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Optimization of an amperometric biosensor array for simultaneous measurement of ethanol, formate, d- and l-lactate JF - Electrochimica Acta N2 - The immobilization of NAD+-dependent dehydrogenases, in combination with a diaphorase, enables the facile development of multiparametric sensing devices. In this work, an amperometric biosensor array for simultaneous determination of ethanol, formate, d- and l-lactate is presented. Enzyme immobilization on platinum thin-film electrodes was realized by chemical cross-linking with glutaraldehyde. The optimization of the sensor performance was investigated with regard to enzyme loading, glutaraldehyde concentration, pH, cofactor concentration and temperature. Under optimal working conditions (potassium phosphate buffer with pH 7.5, 2.5 mmol L-1 NAD+, 2.0 mmol L-1 ferricyanide, 25 °C and 0.4% glutaraldehyde) the linear working range and sensitivity of the four sensor elements was improved. Simultaneous and cross-talk free measurements of four different metabolic parameters were performed successfully. The reliable analytical performance of the biosensor array was demonstrated by application in a clarified sample of inoculum sludge. Thereby, a promising approach for on-site monitoring of fermentation processes is provided. KW - Simultaneous determination KW - Enzymatic biosensor KW - Diaphorase KW - Dehydrogenase Y1 - 2017 U6 - http://dx.doi.org/10.1016/j.electacta.2017.07.119 SN - 0013-4686 VL - 251 SP - 256 EP - 262 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Pilas, Johanna A1 - Mariano, K. A1 - Keusgen, M. A1 - Selmer, Thorsten A1 - Schöning, Michael Josef T1 - Optimization of an Enzyme-based Multi-parameter Biosensor for Monitoring Biogas Processes JF - Procedia Engineering Y1 - 2015 U6 - http://dx.doi.org/10.1016/j.proeng.2015.08.702 SN - 1877-7058 N1 - Part of special issue "Eurosensors 2015" VL - 120 SP - 532 EP - 535 PB - Elsevier CY - Amsterdam ER -