TY - JOUR A1 - Kurz, R. A1 - Linder, Peter A1 - Trzewik, Jürgen A1 - Rüffer, M. A1 - Artmann, Gerhard A1 - Digel, Ilya A1 - Rothermel, A. A1 - Robitzki, A. A1 - Temiz Artmann, Aysegül T1 - Contractile tension and beating rates of self-exciting monolayers and 3D-tissue constructs of neonatal rat cardiomyocytes JF - Medical and Biological Engineering and Computing N2 - The CellDrum technology (The term 'CellDrum technology' includes a couple of slightly different technological setups for measuring lateral mechanical tension in various types of cell monolayers or 3D-tissue constructs) was designed to quantify the contraction rate and mechanical tension of self-exciting cardiac myocytes. Cells were grown either within flexible, circular collagen gels or as monolayer on top of respective 1-mum thin silicone membranes. Membrane and cells were bulged outwards by air pressure. This biaxial strain distribution is rather similar the beating, blood-filled heart. The setup allowed presetting the mechanical residual stress level externally by adjusting the centre deflection, thus, mimicking hypertension in vitro. Tension was measured as oscillating differential pressure change between chamber and environment. A 0.5-mm thick collagen-cardiac myocyte tissue construct induced after 2 days of culturing (initial cell density 2 x 10(4) cells/ml), a mechanical tension of 1.62 +/- 0.17 microN/mm(2). Mechanical load is an important growth regulator in the developing heart, and the orientation and alignment of cardiomyocytes is stress sensitive. Therefore, it was necessary to develop the CellDrum technology with its biaxial stress-strain distribution and defined mechanical boundary conditions. Cells were exposed to strain in two directions, radially and circumferentially, which is similar to biaxial loading in real heart tissues. Thus, from a biomechanical point of view, the system is preferable to previous setups based on uniaxial stretching. Y1 - 2010 U6 - https://doi.org/10.1007/s11517-009-0552-y SN - 1741-0444 VL - 48 IS - 1 SP - 59 EP - 65 PB - Springer Nature CY - Cham ER - TY - JOUR A1 - Kozhalakova, A. A. A1 - Zhubanova, Azhar A. A1 - Mansurov, Z. A. A1 - Digel, Ilya A1 - Tazhibayeva, S. M. A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül T1 - Adsorption of bacterial lipopolysaccharides on carbonized rice shell JF - Science of Central Asia (2010) Y1 - 2010 SP - 50 EP - 54 ER - TY - JOUR A1 - Zhubanova, Azhar A. A1 - Aknazarov, S. K. A1 - Mansurov, Zulkhair A1 - Digel, Ilya A1 - Kozhalakova, A. A. A1 - Akimbekov, Nuraly S. A1 - O'Heras, Carlos A1 - Tazhibayeva, S. A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül T1 - Adsorption of bacterial Lipopolysaccharides and blood plasma proteins on modified carbonized materials N2 - Bacterial lipopolysaccharides (endotoxins) show strong biological effects at very low concentrations in human beings and many animals when entering the blood stream. These include affecting structure and function of organs and cells, changing metabolic functions, raising body temperature, triggering the coagulation cascade, modifying hemodynamics and causing septic shock. Because of this toxicity, the removal of even minute amounts is essential for safe parenteral administration of drugs and also for septic shock patients' care. The absence of a general method for endotoxin removal from liquid interfaces urgently requires finding new methods and materials to overcome this gap. Nanostructured carbonized plant parts is a promising material that showed good adsorption properties due to its vast pore network and high surface area. The aim of this study was comparative measurement of endotoxin- and blood proteins-related adsorption rate and adsorption capacity for different carboneous materials produced at different temperatures and under different surface modifications. As a main surface modificator, positively cbarged polymer, polyethileneimine (PEl) was used. Activated carbon materials showed good adsorption properties for LPS and some proteins used in the experiments. During the batch experiments, several techniques (dust removal, autoclaving) were used and optimized for improving the material's adsorption behavior. Also, with the results obtained it was possible to differentiate the materials according to their adsorption capacity and kinetic characteristics. Modification of the surface apparently has not affected hemoglobin binding to the adsorbent's surface. Obtained adsorption isotherms can be used as a powerful tool for designing of future column-based setups for blood purification from LPS, which is especially important for septic shock treatment. KW - Kohlenstofffaser KW - Lipopolysaccharide KW - nanostrukturierte carbonisierte Pflanzenteile KW - lipopolysaccharides KW - nanostructured carbonized plant parts Y1 - 2010 ER - TY - JOUR A1 - Pham, Phu Tinh A1 - Vu, Khoi Duc A1 - Tran, Thanh Ngoc A1 - Staat, Manfred T1 - A primal-dual algorithm for shakedown analysis of elastic-plastic bounded linearly kinematic hardening bodies Y1 - 2010 N1 - ECCM 2010, IV European Conference on Computational Mechanics, Paris, France, May 16-21, 2010. SP - 1 EP - 7 ER - TY - JOUR A1 - Grottke, O. A1 - Braunschweig, T. A1 - Philippen, B. A1 - Gatzweiler, Karl-Heinz A1 - Gronloh, N. A1 - Staat, Manfred A1 - Rossaint, R. A1 - Tolba, R. T1 - A New Model for Blunt Liver Injuries in the Swine JF - European Surgical Research. 44 (2010), H. 2 Y1 - 2010 SN - 1421-9921 SP - 65 EP - 73 ER - TY - JOUR A1 - Nguyen, N.-H. A1 - Raatschen, Hans-Jürgen A1 - Staat, Manfred T1 - A hyperelastic model of biological tissue materials in tubular organs Y1 - 2010 N1 - ECCM 2010, IV European Conference on Computational Mechanics, Paris, France, May 16-21, 2010. SP - 1 EP - 12 ER - TY - JOUR A1 - Jansen, Sebastian A1 - Behbahani, Mehdi A1 - Laumen, Marco A1 - Kaufmann, Tim A1 - Hormes, Marcus A1 - Schmitz-Rode, Thomas A1 - Behr, Marek A1 - Steinseifer, Ulrich T1 - 3D Stereo-PIV Validation for CFD-Simulation of Steady Flow through the Human Aorta using Rapid-Prototyping techniques Y1 - 2010 N1 - abstract ; IV International Symposium on Modelling of Physiological Flows, Sardinia, Italy, June 02-05, 2010 ; MPF2010 ER -