TY - JOUR
A1 - Feuerriegel, Uwe
A1 - Klose, W.
T1 - Reaktionskinetik der katalytischen Methan-Oxidation und experimentelle Untersuchungen zur Desaktivierung eines Palladium-Trägerkatalysators durch Schwefelwasserstoff. Klose, W. ; Feuerriegel, U.
JF - Chemie - Ingenieur - Technik. 65 (1993), H. 10
Y1 - 1993
SN - 0009-286X
N1 - engl. Titel: Reaction Kinetics of Catalytic Methane Oxidation and Deactivation of a Palladium Catalyst by Hydrogen Sulfide During Catalytic Oxidation of Methane
SP - 1242
EP - 1243
ER -
TY - JOUR
A1 - Feuerriegel, Uwe
A1 - Klose, W.
T1 - Untersuchungen zum Mechanismus der Desaktivierung eines Palladium-Trägerkatalysators durch Vergiftung mit Schwefelwasserstoff
JF - Chemie - Ingenieur - Technik. 70 (1998), H. 4
Y1 - 1998
SN - 0009-286X
N1 - Artikel in Englisch unter Chemical engineering & technology. 21(1998)7. S. 562-564
SP - 449
EP - 452
ER -
TY - JOUR
A1 - Feuerriegel, Uwe
A1 - Klose, W.
T1 - Investigations into the Mechanism of the Deactivation of a Palladium-Supported Catalyst by Hydrogen Sulfide Poisoning. Klose, W. ; Feuerriegel, U.
JF - Chemical engineering & technology. 21 (1998), H. 7
Y1 - 1998
SN - 0930-7516
N1 - gleicher Artikel in Deutsch In: Chem.Ing.Tech 70(1998) No S. 449-452 u.d.T.: Untersuchungen zum Mechanismus der Desaktivierung eines Palladium-Trägerkatalysators durch Vergiftung mit Schwefelwasserstoff
SP - 562
EP - 564
ER -
TY - JOUR
A1 - Feuerriegel, Uwe
A1 - Klose, W.
A1 - Sloboshanin, S.
A1 - Goebel, H. [u.a.]
T1 - Deactivation of a palladium-supported alumina catalyst by hydrogen sulfide during the oxidation of methane
JF - Langmuir: the ACS journal of surfaces and colloids. 10 (1994), H. 10
Y1 - 1994
SN - 0743-74363
SP - 3567
EP - 3570
ER -
TY - JOUR
A1 - Feuerriegel, Uwe
A1 - Künsch, M.
A1 - Stahlberg, R.
A1 - Steiger, F.
T1 - Massen- und Energiebilanz des Thermoselect-Verfahrens
JF - Thermoselect-Verfahren zur Ent- und Vergasung von Abfällen / Franz J. Schweitzer [Hrsg.]
Y1 - 1994
SN - 3-924511-47-0
PB - EF-Verl. für Energie- u. Umwelttechnik
CY - Berlin
ER -
TY - JOUR
A1 - Feuerriegel, Uwe
A1 - Ma, J.
A1 - Hoffmann, U.
T1 - Untersuchungen zur Rußfilterregeneration mit Hilfe von Zündhilfen. Ma, J.; Hoffmann, U.; Feuerriegel, U
JF - Erdöl und Kohle – Erdgas, Brennstoff-Chemie. 43 (1990)
Y1 - 1990
SN - 0014-0058
N1 - Druckausgabe in Jülich unter 63 ZS 034-1990 vorhanden
SP - 150
EP - 157
ER -
TY - CHAP
A1 - Feuerriegel, Uwe
A1 - Pook, Michael
A1 - Wersch, Gregor
A1 - Wittenhorst, Simon
A1 - Becker, Jürgen
A1 - Ecker, Markus
A1 - Hoffmann, Ulrich
A1 - Kunz, Ulrich
T1 - Simulation von Wärmeübertragungsprozessen
N2 - Mit freundlicher Genehmigung der Autoren und des Oldenbourg Industrieverlags https://www.oldenbourg-industrieverlag.de/de/9783835633223-33223 erschienen als Beitrag im Tagungsband zur AALE-Tagung 2012. 9. Fachkonferenz 4.-5. Mai 2012, Aachen, Fachhochschule. ISBN 9783835633223 S 5-1 S. 127-135 Es werden Ergebnisse unterschiedlicher Projekte aus dem Bereich der Simulation von Wärmeübertragungsprozessen mit Excel-VBA vorgestellt. - Thermische Behandlung hochviskoser Fruchtzubereitungen, verschiedene Projekte und Kooperationen mit der Zentis GmbH & Co. KG, Aachen (J. Becker, U. Feuerriegel, G. Wersch). - Untersuchung des dynamischen Verhaltens von dampfbeheizten Ethylen-Verdampfern. Projekt mit der TGE Gas Engineering GmbH, Bonn (M. Ecker, U. Feuerriegel, U. Hoffmann, S. Wittenhorst). - Dynamische Simulation des axialen Temperaturverlaufs von elektrisch beheizten Rohrreaktoren. Kooperation mit dem Institut für Chemische Verfahrenstechnik, TU Clausthal (U. Feuerriegel, U. Kunz, M. Pook, S. Wittenhorst).
KW - Wärmeübertragung
Y1 - 2012
ER -
TY - JOUR
A1 - Feuerriegel, Uwe
A1 - Stahlberg, R.
T1 - Performance of the Thermoselect Demonstration Plant at Fondotoce, Italy: Product Quality and Distribution of Chemical Elements in the Material Flow. Stahlberg, R. ; Feuerriegel, U.
JF - Solid waste management: thermal treatment & waste-to-energy technologies : proceedings of an international specialty conference sponsored by the Air & Waste Management Association, [Washington, DC, April 18 - 21, 1995] / comp. by James D. Kilgroe
Y1 - 1996
N1 - International Conference on Solid Waste Management, Thermal Treatment and Waste to Energy ; <1995, Washington, DC>
PB - Air & Waste Management Assoc.
CY - Pittsburgh, Pa.
ER -
TY - JOUR
A1 - Feuerriegel, Uwe
A1 - Stahlberg, R.
T1 - Thermoselect – Energie- und Rohstoffgewinnung: Teil 1. Verfahrensgrundlagen zur unterbrechungslosen Verwertung von Restabfällen. Stahlberg, R. ; Feuerriegel, U.
JF - Chemische Technik . 46 (1994), H. 5
Y1 - 1994
SN - 0045-6519
SP - 257
EP - 266
ER -
TY - JOUR
A1 - Feuerriegel, Uwe
A1 - Stahlberg, R.
T1 - Thermoselect – Energie- und Rohstoffgewinnung: Teil 2. Untersuchungen an der Thermoselect-Anlage Fondotoce (Italien), Qualitäten der Produkte und Nachweis der Verteilung der chemischen Elemente in den Stoffströmen. Stahlberg, R. ; Feuerriegel, U.
JF - Chemische Technik . 47 (1995), H. 1
Y1 - 1995
SN - 0045-6519
SP - 1
EP - 10
ER -
TY - JOUR
A1 - Feuerriegel, Uwe
A1 - Stahlberg, R.
T1 - Das Thermoselect-Verfahren zur Energie- und Rohstoffgewinnung – Konzepte, Verfahren, Kosten
JF - Thermische Abfallentsorgung – Konzepte, Kosten, Verfahren. Tagung Veitshöchheim, 27./28.06.1995, VDI-Gesellschaft Energietechnik
Y1 - 1995
SN - 0083-5560
N1 - VDI-Bericht 1192
SP - 319
EP - 319
PB - VDI-Verl.
CY - Düsseldorf
ER -
TY - JOUR
A1 - Feuerriegel, Uwe
A1 - Stahlberg, R.
T1 - Thermoselect – Energie- und Rohstoffgewinnung, Verfahrensgrundlagen zur unterbrechungslosen Verwertung von Restabfällen. Stahlberg, R.; Feuerriegel, U.
JF - Thermoselect : der neue Weg, Restmüll umweltgerecht zu behandeln ; [Tagungsband zur Fachtagung Thermoselect am 19. Januar 1995 in Rastatt/Baden] / Günther Häßler. 2. durchges. und erw. Aufl.
Y1 - 1995
SN - 3-9803665-4-5
N1 - Fachtagung THERMOSELECT ; (1995, Rastatt) ; Fachtagung Thermoselect ; (1995.01.19 : ; Rastatt)
SP - 13
EP - 32
PB - Goerner
CY - Karlsruhe
ER -
TY - JOUR
A1 - Feuerriegel, Uwe
A1 - Stahlberg, R.
T1 - Untersuchungen an der Thermoselect-Anlage Fondotoce (Italien), Qualitäten der Produkte und Nachweis der Verteilung der chemischen Elemente in den Stoffströmen. Stahlberg, R.; Feuerriegel, U.
JF - Thermoselect : der neue Weg, Restmüll umweltgerecht zu behandeln ; [Tagungsband zur Fachtagung Thermoselect am 19. Januar 1995 in Rastatt/Baden] / Günther Häßler. 2. durchges. und erw. Aufl.
Y1 - 1995
SN - 3-9803665-4-5
N1 - Fachtagung THERMOSELECT ; (1995, Rastatt) ; Fachtagung Thermoselect ; (1995.01.19 : ; Rastatt)
SP - 33
EP - 54
PB - Goerner
CY - Karlsruhe
ER -
TY - JOUR
A1 - Feuerriegel, Uwe
A1 - Stahlberg, R.
T1 - Thermoselect – Energie- und Rohstoffgewinnung. Stahlberg, R. ; Feuerriegel, U.
JF - Abfallwirtschaft Stoffkreisläufe / Terratec '95, Kongress West-Ost-Transfer Umwelt vom 1. bis 3. März 1995. Leipziger Messe. Hrsg. von Hasso Scheffler ...
Y1 - 1995
SN - 3-8154-3513-7
N1 - Terratec <1995, Leipzig> ; Kongress West-Ost-Transfer Umwelt <1995, Leipzig> ; Leipziger Messe
SP - 179
EP - 194
PB - Teubner
CY - Stuttgart [u.a.]
ER -
TY - JOUR
A1 - Feuerriegel, Uwe
A1 - Stahlberg, R.
A1 - Riegel, J.
A1 - Steiger, F.
T1 - Thermoselect – Energie- und Rohstoffgewinnung: Teil 3. Massen- und Energiebilanz unter Berücksichtigung der Wirtschaftlichkeit der Synthesegasnutzung. Stahlberg, R.; Riegel, J.; Feuerriegel, U.; Steiger, F.
JF - Chemische Technik . 47 (1995), H. 3
Y1 - 1995
SN - 0045-6519
SP - 113
EP - 121
ER -
TY - JOUR
A1 - Feuerriegel, Uwe
A1 - Stahlberg, R.
A1 - Weisenburger, P.
A1 - Steiger, F.
T1 - Massen- und Energiebilanzen unter Einbeziehung der Untersuchungen an der Thermoselect-Anlage Fondotoce (Italien). Stahlberg, R.; Feuerriegel, U.; Weisenburger, P.; Steiger, F.
JF - Thermoselect : der neue Weg, Restmüll umweltgerecht zu behandeln ; [Tagungsband zur Fachtagung Thermoselect am 19. Januar 1995 in Rastatt/Baden] / Günther Häßler. 2. durchges. und erw. Aufl.
Y1 - 1995
SN - 3-9803665-4-5
N1 - Fachtagung THERMOSELECT ; (1995, Rastatt) ; Fachtagung Thermoselect ; (1995.01.19 : ; Rastatt)
SP - 55
EP - 84
PB - Goerner
CY - Karlsruhe
ER -
TY - CHAP
A1 - Feuerriegel, Uwe
A1 - Wittenhorst, Simon
A1 - Hoffmann, Ulrich
A1 - Pook, Michael
T1 - Simulation von Wärmeübertragungsprozessen
T2 - Tagungsband zur AALE-Tagung 2012 : 9. Fachkonferenz
Y1 - 2012
SN - 978-3-8356-3305-6
N1 - AALE 2012
SP - 127
EP - 136
PB - Oldenbourg Industrieverlag
CY - München
ER -
TY - PAT
A1 - Finger, Sebastian
A1 - Hojdis, Nils
A1 - Menglong, Huang
T1 - Sekundäre galvanische Zelle
N2 - Die vorliegende Erfindung betrifft eine sekundäre galvanische Zelle, umfassend eine Kathode, eine Anode und einen Separator, der zwischen der Kathode und der Anode angeordnet ist, wobei die Kathode ein erstes elastomeres Polymer umfasst, welches mit einem ersten Füllstoff als Kathodenmaterial gefüllt ist, wobei die Anode ein zweites elastomeres Polymer umfasst, welches mit einem zweiten Füllstoff als Anodenmaterial gefüllt ist, wobei der Separator ein drittes elastomeres Polymer umfasst, wobei das erste elastomere Polymer, das zweite elastomere Polymer und das dritte elastomere Polymer unabhängig voneinander aus vernetzungsfähigen Dienkautschuken ausgewählt sind, und wobei zumindest eines von dem ersten elastomeren Polymer, dem zweiten elastomeren Polymer und dem dritten elastomeren Polymer eine ionische Flüssigkeit und/oder ein elektrisch leitfähiges Polymer enthält. Die vorliegende Erfindung betrifft ferner eine wiederaufladbare Batterie, umfassend die erfindungsgemäße sekundäre galvanische Zelle, ein Verfahren zur Herstellung einer sekundären galvanischen Zelle sowie die Verwendung der erfindungsgemäßen sekundären galvanischen Zelle sowie der erfindungsgemäßen wiederaufladbaren Batterie.
Y1 - 2020
N1 - Patent DE102018222385A1 25.06.2020
auch veröffentlicht unter EP3671932A1
ER -
TY - PAT
A1 - Friederici, Nadja-Annette
A1 - Hojdis, Nils
T1 - Fahrzeugreifen
N2 - Die Erfindung betrifft einen Fahrzeugreifen mit zumindest einem radial außen befindlichen Gummilaufstreifen, Wulstbereichen für den Anschluss an eine Felge und mit auf einer Gummimischung basierenden Seitenwandbereichen zwischen Gummilaufstreifen und den Wulstbereichen. Ferner betrifft die Erfindung Verfahren zur Herstellung solcher Fahrzeugreifen. Um den Anteil der umweltschädlichen Substanzen, insbesondere im Innenstadtbereich, zu reduzieren weisen die Seitenwandbereiche auf der äußeren Oberfläche eine für den oxidativen Abbau von Molekülen photokatalytisch aktive Substanz auf.
Y1 - 2018
N1 - Patent DE102016219338A1 04.12.2018
ER -
TY - CHAP
A1 - Frotscher, Ralf
A1 - Goßmann, Matthias
A1 - Raatschen, Hans-Jürgen
A1 - Temiz Artmann, Aysegül
A1 - Staat, Manfred
T1 - Simulation of cardiac cell-seeded membranes using the edge-based smoothed FEM
T2 - Shell and membrane theories in mechanics and biology. (Advanced structured materials ; 45)
N2 - We present an electromechanically coupled Finite Element model for cardiac tissue. It bases on the mechanical model for cardiac tissue of Hunter et al. that we couple to the McAllister-Noble-Tsien electrophysiological model of purkinje fibre cells. The corresponding system of ordinary differential equations is implemented on the level of the constitutive equations in a geometrically and physically nonlinear version of the so-called edge-based smoothed FEM for plates. Mechanical material parameters are determined from our own pressure-deflection experimental setup. The main purpose of the model is to further examine the experimental results not only on mechanical but also on electrophysiological level down to ion channel gates. Moreover, we present first drug treatment simulations and validate the model with respect to the experiments.
Y1 - 2015
SN - 978-3-319-02534-6 ; 978-3-319-02535-3
SP - 187
EP - 212
PB - Springer
CY - Heidelberg
ER -
TY - JOUR
A1 - Gebeshuber, Christoph A.
A1 - Kornauth, Christoph
A1 - Dong, Lihua
A1 - Sierig, Ralph
A1 - Seibler, Jost
A1 - Reiss, Martina
A1 - Tauber, Stefanie
A1 - Bilban, Martin
A1 - Wang, Shijun
A1 - Kain, Renate
A1 - Böhmig, Georg A.
A1 - Moeller, Marcus J.
A1 - Gröne, Hermann-Josef
A1 - Englert, Christoph
A1 - Martinez, Javier
A1 - Kerjaschki, Dontscho
T1 - Focal segmental glomerulosclerosis is induced by microRNA-193a and its downregulation of WT1
JF - Nature Medicine
Y1 - 2013
U6 - https://doi.org/10.1038/nm.3142
SN - 1078-8956
VL - 19
IS - 4
SP - 481
EP - 487
ER -
TY - JOUR
A1 - Gerigk, M.
A1 - Bujnicki, R.
A1 - Ganpo-Nkwenkwa, E.
A1 - Bongaerts, Johannes
A1 - Sprenger, G.
A1 - Takors, Ralf
T1 - Process control for enhanced L-phenylalanine production using different recombinant Escherichia coli strains
JF - Biotechnology and bioengineering
Y1 - 2002
SN - 1097-0290 (E-Journal); 0006-3592 (Print)
VL - Vol. 80
IS - Iss. 7
SP - 746
EP - 754
ER -
TY - JOUR
A1 - Gerigk, M.
A1 - Maaß, D.
A1 - Kreutzer, A.
A1 - Sprenger, G.
A1 - Bongaerts, Johannes
A1 - Wubbolts, Marcel
A1 - Takors, Ralf
T1 - Enhanced pilot-scale fed-batch L-phenylalanine production with recombinant Escherichia coli by fully integrated reactive extraction
JF - Bioprocess and biosystems engineering
Y1 - 2002
SN - 1432-0797 (E-Journal); 1615-7605 (E-Journal); 0178-515X (Print); 1615-7591 (Print)
VL - Vol. 25
IS - Iss. 1
SP - 43
EP - 52
ER -
TY - JOUR
A1 - Gerigk, M.
A1 - Maaß, D.
A1 - Takors, Ralf
A1 - Kreutzer, A.
A1 - Wandrey, Christian
A1 - Bongaerts, Johannes
A1 - Wubbolts, Marcel
T1 - Fermentative Herstellung von L-Phenylalanin im Fed-Batch Verfahren mit E. coli unter Einbindung eines integrierten Aufarbeitungsverfahrens
JF - Chemie-Ingenieur-Technik (CIT)
Y1 - 2000
SN - 1522-2640 (E-Journal); 0009-286X (Print)
N1 - Printausg. in der Bibliothek vorhanden: 63 ZS 022
VL - Vol. 72
IS - Iss. 9
SP - 926
EP - 927
ER -
TY - JOUR
A1 - Ghosch, S.
A1 - Baier, M.
A1 - Schütz, J.
A1 - Schneider, Felix
A1 - Scherer, Ulrich W.
T1 - Analysis of electronic autoradiographs by mathematical post-processing
JF - Radiation Effects and Defects in Solids: Incorporating plasma science and plasma technology
N2 - Autoradiography is a well-established method of nuclear imaging. When different radionuclides are present simultaneously, additional processing is needed to distinguish distributions of radionuclides. In this work, a method is presented where aluminium absorbers of different thickness are used to produce images with different cut-off energies. By subtracting images pixel-by-pixel one can generate images representing certain ranges of β-particle energies. The method is applied to the measurement of irradiated reactor graphite samples containing several radionuclides to determine the spatial distribution of these radionuclides within pre-defined energy windows. The process was repeated under fixed parameters after thermal treatment of the samples. The greyscale images of the distribution after treatment were subtracted from the corresponding pre-treatment images. Significant changes in the intensity and distribution of radionuclides could be observed in some samples. Due to the thermal treatment parameters the most significant differences were observed in the ³H and ¹⁴C inventory and distribution.
Y1 - 2016
U6 - https://doi.org/10.1080/10420150.2016.1155587
SN - 1029-4953
VL - 171
IS - 1-2
SP - 161
EP - 172
PB - Taylor & Francis
CY - London
ER -
TY - JOUR
A1 - Gielen, Hans-Günther
A1 - Wolf, Günter
A1 - Berndt, Heinz
A1 - Zahn, Helmut
T1 - Synthese der Fragmente A1–8, A9–15 und A16–21 der Schafinsulin-A-Kette unter Verwendung des S-tert-Butylmercaptorestes als Thiolschutzgruppe
JF - Hoppe-Seyler's Zeitschrift für physiologische Chemie
Y1 - 1979
U6 - https://doi.org/10.1515/bchm2.1979.360.2.1535
SN - 1437-4315
SN - 0018-4888
VL - 360
IS - 2
SP - 1535
EP - 1548
ER -
TY - BOOK
A1 - Gilch, Heinz Gerhard
A1 - Rath, Walter
T1 - Moisture-curing, polyurethane hot-melt compositions = Feuchtigkeitshärtende Polyurethan-Schmelzklebstoff-Zusammensetzungen
Y1 - 1995
N1 - EP 0369607 30.03.1995 Veröffentlichungstag im Patentblatt ; Volltext recherchierbar über:
PB - Europäisches Patentamt
CY - München
ER -
TY - JOUR
A1 - Glaser, Stefan
A1 - Lubitz, Sandra
A1 - Loveland, Kate L.
A1 - Ohbo, Kazu
A1 - Robb, Lorraine
A1 - Schwenk, Frieder
A1 - Seibler, Jost
A1 - Roellig, Daniela
A1 - Kranz, Andrea
A1 - Anastassiadis, Konstantinos
A1 - Stewart, A. Francis
T1 - The histone 3 lysine 4 methyltransferase, Mll2, is only required briefly in development and spermatogenesis
JF - Epigenetics & Chromatin
Y1 - 2009
U6 - https://doi.org/10.1186/1756-8935-2-5
SN - 1756-8935
VL - 2
IS - 5
SP - 1
EP - 16
ER -
TY - JOUR
A1 - Goetze, Sandra
A1 - Baer, Alexandra
A1 - Winkelmann, Silke
A1 - Nehlsen, Kristina
A1 - Seibler, Jost
A1 - Maass, Karin
A1 - Bode, Jürgen
T1 - Performance of genomic bordering elements at predefined genomic loci
JF - Molecular and Cellular Biology
Y1 - 2005
U6 - https://doi.org/10.1128/MCB.25.6.2260-2272.2005
SN - 1098-5549
VL - 25
IS - 6
SP - 2260
EP - 2272
ER -
TY - GEN
A1 - Graf, Alain-Michel
A1 - Steinhof, Rafael
A1 - Lotz, Martin
A1 - Tippkötter, Nils
A1 - Kasper, Cornelia
A1 - Beutel, Sascha
A1 - Ulber, Roland
T1 - Downstream-Processing mit Membranadsorbern zur Isolierung nativer Proteinfraktionen aus Kartoffelfruchtwasser
T2 - Chemie Ingenieur Technik
N2 - Bei der Stärkeproduktion entstehendes Kartoffelfruchtwasser besitzt mit 2 – 3 % einen hohen Anteil an ernährungsphysiologisch interessanten Proteinen. Die industrielle Gewinnung dieser Proteinfracht liefert jedoch lediglich ein minderwertiges, denaturiertes Produkt. Mit Hilfe der Membranadsorber-Technologie lassen sich aus Kartoffelfruchtwasser unter milden Reaktionsbedingungen native bioaktive Proteinfraktionen gewinnen. Geeignete Trennbedingungen wurden im Labormaßstab entwickelt und in den Technikumsmaßstab übertragen. An Anionenaustauscher-Membranadsorbern mit einer Membranfläche von 10 000 cm2 wurde eine Patatinhaltige Fraktion (44 kDa) mit Bindungskapazitäten von 0,37 mg/cm2 isoliert. Eine niedermolekulare Proteinfraktion mit Protease-Inhibitoren konnte durch Kationenaustauscher-Membranadsorber mit Bindungskapazitäten von 1,00 mg/cm2 gewonnen werden. Sie ist für verschiedenste Applikationen in der pharmazeutischen, kosmetischen und der Nahrungsmittelindustrie interessant z. B. für Appetitzügler oder muskelaufbauende Proteinpräparate. Der Aufreinigung der nativen Proteinfraktionen durch Ultra-/Diafiltration schließt sich die Konfektionierung durch Sprühtrocknung an. Die bioanalytische Charakterisierung der Produkte belegt die Reinheit und die enzymatische Aktivität sowie die Abreicherung von Störkomponenten wie Glykoalkaloide und Polyphenoloxidasen.
Y1 - 2009
U6 - https://doi.org/10.1002/cite.200800139
VL - 81
IS - 3
SP - 267
EP - 274
PB - Wiley
CY - Weinheim
ER -
TY - INPR
A1 - Greiner, Lasse
A1 - Jeromin, Günter Erich
A1 - Sithole, Patience
A1 - Petersen, Soenke
T1 - Preprint: Studies on the enzymatic reduction of levulinic acid using Chiralidon-R and Chiralidon-S
T2 - ChemRxiv
N2 - The enzymatic reduction of levulinic acid by the chiral catalysts Chiralidon-R and Chiralidon-S which are commercially available superabsorbed alcohol dehydrogenases is described. The Chiralidon®-R/S reduces the levulinic acid to the (R,S)-4-hydroxy valeric acid and the (R)- or (S)- gamma-valerolactone.
KW - Levulinic acid
KW - Chiralidon-R
KW - Chiralidon-S
KW - 4-hydroxy valeric acid
KW - (R)- or (S)- gamma-valerolactone
Y1 - 2023
U6 - https://doi.org/10.26434/chemrxiv-2023-jlvcv
ER -
TY - JOUR
A1 - Großhauser, Christian
A1 - Pettrak, Jürgen
T1 - Die Rolle des Wasserstoffs in der Abwasserbehandlung
JF - Wasser und Abfall
N2 - Die Bereitstellung von nachhaltig erzeugtem Wasserstoff als Energieträger und Rohstoff ist eine wichtige Schlüsseltechnologie sowohl als Ersatz für fossile Energieträger, aber auch als Produkt im Zusammenhang mit Kreislaufprozessen. In der Abwasserbehandlung bestehen verschiedene Möglichkeiten Wasserstoff herzustellen. Mehrere Wege, mögliche Synergien, aber auch deren Nachteile werden vorgestellt.
Y1 - 2023
U6 - https://doi.org/10.1007/s35152-023-1444-4
SN - 1436-9095
SN - 2192-8754 (eISSN)
IS - 7/8
PB - Springer Fachmedien
CY - Wiesbaden
ER -
TY - JOUR
A1 - Guo, Yuanyuan
A1 - Miyamoto, Ko-ichiro
A1 - Wagner, Torsten
A1 - Schöning, Michael Josef
A1 - Yoshinobu, Tatsuo
T1 - Theoretical study and simulation of light-addressable potentiometric sensors
JF - Physica status solidi (A) : applications and materials
N2 - The light-addressable potentiometric sensor (LAPS) is a semiconductor-based potentiometric sensor using a light probe with an ability of detecting the concentration of biochemical species in a spatially resolved manner. As an important biomedical sensor, research has been conducted to improve its performance, for instance, to realize high-speed measurement. In this work, the idea of facilitating the device-level simulation, instead of using an equivalent-circuit model, is presented for detailed analysis and optimization of the performance of the LAPS. Both carrier distribution and photocurrent response have been simulated to provide new insight into both amplitude-mode and phase-mode operations of the LAPS. Various device parameters can be examined to effectively design and optimize the LAPS structures and setups for enhanced performance.
Y1 - 2014
U6 - https://doi.org/10.1002/pssa.201330354
SN - 0031-8965
VL - 211
IS - 6
SP - 1467
EP - 1472
PB - Wiley-VCH
CY - Weinheim
ER -
TY - RPRT
A1 - Haeger, Gerrit
A1 - Bongaerts, Johannes
A1 - Siegert, Petra
T1 - Abschlussbericht Teil II: Eingehende Darstellung Neue biobasierte Lipopeptide aus nachhaltiger Produktion (LipoPep)
Y1 - 2023
N1 - Förderkennzeichen: 13FH256PA6
Titel: FHprofUnt 2016: Neue biobasierte Lipopeptide aus nachhaltiger Produktion
Laufzeit: 01.02.2019 – 31.10.2022
ER -
TY - JOUR
A1 - Haeger, Gerrit
A1 - Bongaerts, Johannes
A1 - Siegert, Petra
T1 - A convenient ninhydrin assay in 96-well format for amino acid-releasing enzymes using an air-stable reagent
JF - Analytical Biochemistry
N2 - An improved and convenient ninhydrin assay for aminoacylase activity measurements was developed using the commercial EZ Nin™ reagent. Alternative reagents from literature were also evaluated and compared. The addition of DMSO to the reagent enhanced the solubility of Ruhemann's purple (RP). Furthermore, we found that the use of a basic, aqueous buffer enhances stability of RP. An acidic protocol for the quantification of lysine was developed by addition of glacial acetic acid. The assay allows for parallel processing in a 96-well format with measurements microtiter plates.
Y1 - 2022
U6 - https://doi.org/10.1016/j.ab.2022.114819
SN - 1096-0309
IS - 624
PB - Elsevier
CY - Amsterdam
ER -
TY - JOUR
A1 - Haeger, Gerrit
A1 - Grankin, Alina
A1 - Wagner, Michaela
T1 - Construction of an Aspergillus oryzae triple amylase deletion mutant as a chassis to evaluate industrially relevant amylases using multiplex CRISPR/Cas9 editing technology
JF - Applied Research
N2 - Aspergillus oryzae is an industrially relevant organism for the secretory production of heterologous enzymes, especially amylases. The activities of potential heterologous amylases, however, cannot be quantified directly from the supernatant due to the high background activity of native α-amylase. This activity is caused by the gene products of amyA, amyB, and amyC. In this study, an in vitro CRISPR/Cas9 system was established in A. oryzae to delete these genes simultaneously. First, pyrG of A. oryzae NSAR1 was mutated by exploiting NHEJ to generate a counter-selection marker. Next, all amylase genes were deleted simultaneously by co-transforming a repair template carrying pyrG of Aspergillus nidulans and flanking sequences of amylase gene loci. The rate of obtained triple knock-outs was 47%. We showed that triple knockouts do not retain any amylase activity in the supernatant. The established in vitro CRISPR/Cas9 system was used to achieve sequence-specific knock-in of target genes. The system was intended to incorporate a single copy of the gene of interest into the desired host for the development of screening methods. Therefore, an integration cassette for the heterologous Fpi amylase was designed to specifically target the amyB locus. The site-specific integration rate of the plasmid was 78%, with exceptional additional integrations. Integration frequency was assessed via qPCR and directly correlated with heterologous amylase activity. Hence, we could compare the efficiency between two different signal peptides. In summary, we present a strategy to exploit CRISPR/Cas9 for gene mutation, multiplex knock-out, and the targeted knock-in of an expression cassette in A. oryzae. Our system provides straightforward strain engineering and paves the way for development of fungal screening systems.
KW - aspergillus
KW - CRISPR/Cas9
KW - filamentous fungi
KW - genome engineering
Y1 - 2023
U6 - https://doi.org/10.1002/appl.202200106
SN - 2702-4288
IS - Early View
SP - 1
EP - 15
PB - Wiley-VCH
ER -
TY - JOUR
A1 - Haeger, Gerrit
A1 - Probst, Johanna
A1 - Jaeger, Karl-Erich
A1 - Bongaerts, Johannes
A1 - Siegert, Petra
T1 - Novel aminoacylases from Streptomyces griseus DSM 40236 and their recombinant production in Streptomyces lividans
JF - FEBS Open Bio
N2 - Amino acid-based surfactants are valuable compounds for cosmetic formulations. The chemical synthesis of acyl-amino acids is conventionally performed by the Schotten-Baumann reaction using fatty acyl chlorides, but aminoacylases have also been investigated for use in biocatalytic synthesis with free fatty acids. Aminoacylases and their properties are diverse; they belong to different peptidase families and show differences in substrate specificity and biocatalytic potential. Bacterial aminoacylases capable of synthesis have been isolated from Burkholderia, Mycolicibacterium, and Streptomyces. Although several proteases and peptidases from S. griseus have been described, no aminoacylases from this species have been identified yet. In this study, we investigated two novel enzymes produced by S. griseus DSM 40236ᵀ . We identified and cloned the respective genes and recombinantly expressed an α-aminoacylase (EC 3.5.1.14), designated SgAA, and an ε-lysine acylase (EC 3.5.1.17), designated SgELA, in S. lividans TK23. The purified aminoacylase SgAA was biochemically characterized, focusing on its hydrolytic activity to determine temperature- and pH optima and stabilities. The aminoacylase could hydrolyze various acetyl-amino acids at the Nα -position with a broad specificity regarding the sidechain. Substrates with longer acyl chains, like lauroyl-amino acids, were hydrolyzed to a lesser extent. Purified aminoacylase SgELA specific for the hydrolysis of Nε -acetyl-L-lysine was unstable and lost its enzymatic activity upon storage for a longer period but could initially be characterized. The pH optimum of SgELA was pH 8.0. While synthesis of acyl-amino acids was not observed with SgELA, SgAA catalyzed the synthesis of lauroyl-methionine.
KW - Streptomyces lividans
KW - recombinant expression
KW - Streptomyces griseus
KW - ε-lysine acylase
KW - α-aminoacylase
Y1 - 2023
U6 - https://doi.org/10.1002/2211-5463.13723
SN - 2211-5463
N1 - Corresponding author: Petra Siegert
VL - 13
IS - 12
SP - 2224
EP - 2238
PB - Wiley
CY - Hoboken, NJ
ER -
TY - JOUR
A1 - Hafidi, Youssef
A1 - El Hatka, Hicham
A1 - Schmitz, Dominik
A1 - Krauss, Manuel
A1 - Pettrak, Jürgen
A1 - Biel, Markus
A1 - Ittobane, Najim
T1 - Sustainable soil additives for water and micronutrient supply: swelling and chelating properties of polyaspartic acid hydrogels utilizing newly developed crosslinkers
JF - Gels
N2 - Drought and water shortage are serious problems in many arid and semi-arid regions. This problem is getting worse and even continues in temperate climatic regions due to climate change. To address this problem, the use of biodegradable hydrogels is increasingly important for the application as water-retaining additives in soil. Furthermore, efficient (micro-)nutrient supply can be provided by the use of tailored hydrogels. Biodegradable polyaspartic acid (PASP) hydrogels with different available (1,6-hexamethylene diamine (HMD) and L-lysine (LYS)) and newly developed crosslinkers based on diesters of glycine (GLY) and (di-)ethylene glycol (DEG and EG, respectively) were synthesized and characterized using Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) and regarding their swelling properties (kinetic, absorbency under load (AUL)) as well as biodegradability of PASP hydrogel. Copper (II) and zinc (II), respectively, were loaded as micronutrients in two different approaches: in situ with crosslinking and subsequent loading of prepared hydrogels. The results showed successful syntheses of di-glycine-ester-based crosslinkers. Hydrogels with good water-absorbing properties were formed. Moreover, the developed crosslinking agents in combination with the specific reaction conditions resulted in higher water absorbency with increased crosslinker content used in synthesis (10% vs. 20%). The prepared hydrogels are candidates for water-storing soil additives due to the biodegradability of PASP, which is shown in an exemple. The incorporation of Cu(II) and Zn(II) ions can provide these micronutrients for plant growth.
KW - micronutrients
KW - swelling properties
KW - biodegradable polymers
KW - hydrogels
KW - superabsorbent polymers
KW - glycine
KW - polyaspartic acid
Y1 - 2024
U6 - https://doi.org/10.3390/gels10030170
SN - 2310-2861
VL - 10
IS - 3
SP - Artikel 170
PB - MDPI
CY - Basel
ER -
TY - JOUR
A1 - Hager, Jonathan
A1 - Hentschke, Reinhard
A1 - Hojdis, Nils
A1 - Karimi-Varzaneh, Hossein Ali
T1 - Computer Simulation of Particle–Particle Interaction in a Model Polymer Nanocomposite
JF - Macromolecules
Y1 - 2015
U6 - https://doi.org/10.1021/acs.macromol.5b01864
SN - 1520-5835
VL - 48
IS - 24
SP - 9039
EP - 9049
ER -
TY - CHAP
A1 - Hahn, Thomas
A1 - Kelly, Svenja
A1 - Muffler, Kai
A1 - Tippkötter, Nils
A1 - Ulber, Roland
ED - Hans-Jörg, Bart
ED - Pilz, Stephan
T1 - Extraction of lignocellulose and algae for the production of bulk and fine chemicals
T2 - Industrial scale natural products extraction
Y1 - 2011
SN - 978-3-527-32504-7 (Print)
SN - 978-3-527-63512-2 (Online)
U6 - https://doi.org/10.1002/9783527635122
SP - 221
EP - 245
PB - Wiley-VCH
CY - Weinheim
ER -
TY - JOUR
A1 - Halbach, Thorsten
A1 - Scheer, Nico
T1 - Transcriptional activation by the PHD finger is inhibited through an adjacent leucine zipper that binds 14-3-3 proteins
JF - Nucleic Acids Research
Y1 - 2000
U6 - https://doi.org/10.1093/nar/28.18.3542
SN - 1362-4962
VL - 28
IS - 18
SP - 3542
EP - 3550
ER -
TY - JOUR
A1 - Handtke, Stefan
A1 - Schroeter, Rebecca
A1 - Jürgen, Britta
A1 - Methling, Karen
A1 - Schlüter, Rabea
A1 - Albrecht, Dirk
A1 - Hijum, Sacha A. F. T. van
A1 - Bongaerts, Johannes
A1 - Maurer, Karl-Heinz
A1 - Lalk, Michael
A1 - Schweder, Thomas
A1 - Hecker, Michael
A1 - Voigt, Birgit
T1 - Bacillus pumilus reveals a remarkably high resistance to hydrogen peroxide provoked oxidative stress
JF - PLOS one
N2 - Bacillus pumilus is characterized by a higher oxidative stress resistance than other comparable industrially relevant Bacilli such as B. subtilis or B. licheniformis. In this study the response of B. pumilus to oxidative stress was investigated during a treatment with high concentrations of hydrogen peroxide at the proteome, transcriptome and metabolome level. Genes/proteins belonging to regulons, which are known to have important functions in the oxidative stress response of other organisms, were found to be upregulated, such as the Fur, Spx, SOS or CtsR regulon. Strikingly, parts of the fundamental PerR regulon responding to peroxide stress in B. subtilis are not encoded in the B. pumilus genome. Thus, B. pumilus misses the catalase KatA, the DNA-protection protein MrgA or the alkyl hydroperoxide reductase AhpCF. Data of this study suggests that the catalase KatX2 takes over the function of the missing KatA in the oxidative stress response of B. pumilus. The genome-wide expression analysis revealed an induction of bacillithiol (Cys-GlcN-malate, BSH) relevant genes. An analysis of the intracellular metabolites detected high intracellular levels of this protective metabolite, which indicates the importance of bacillithiol in the peroxide stress resistance of B. pumilus.
Y1 - 2014
U6 - https://doi.org/10.1371/journal.pone.0085625
SN - 1932-6203
VL - 9
IS - 1
PB - PLOS
CY - San Francisco
ER -
TY - JOUR
A1 - Handtke, Stefan
A1 - Volland, Sonja
A1 - Methling, Karen
A1 - Albrecht, Dirk
A1 - Becher, Dörte
A1 - Nehls, Jenny
A1 - Bongaerts, Johannes
A1 - Maurer, Karl-Heinz
A1 - Lalk, Michael
A1 - Liesegang, Heiko
A1 - Voigt, Birgit
A1 - Daniel, Rolf
A1 - Hecker, Michael
T1 - Cell physiology of the biotechnological relevant bacterium Bacillus pumilus - An omics-based approach
JF - Journal of Biotechnology
N2 - Members of the species Bacillus pumilus get more and more in focus of the biotechnological industry as potential new production strains. Based on exoproteome analysis, B. pumilus strain Jo2, possessing a high secretion capability, was chosen for an omics-based investigation. The proteome and metabolome of B. pumilus cells growing either in minimal or complex medium was analyzed. In total, 1542 proteins were identified in growing B. pumilus cells, among them 1182 cytosolic proteins, 297 membrane and lipoproteins and 63 secreted proteins. This accounts for about 43% of the 3616 proteins encoded in the B. pumilus Jo2 genome sequence. By using GC–MS, IP-LC/MS and H NMR methods numerous metabolites were analyzed and assigned to reconstructed metabolic pathways. In the genome sequence a functional secretion system including the components of the Sec- and Tat-secretion machinery was found. Analysis of the exoproteome revealed secretion of about 70 proteins with predicted secretion signals. In addition, selected production-relevant genome features such as restriction modification systems and NRPS clusters of B. pumilus Jo2 are discussed.
Y1 - 2014
U6 - https://doi.org/10.1016/j.jbiotec.2014.08.028
SN - 1873-4863 (E-Journal); 0168-1656 (Print)
IS - 192(A)
SP - 204
EP - 214
PB - Elsevier
CY - Amsterdam
ER -
TY - JOUR
A1 - Hans, Stefan
A1 - Scheer, Nico
A1 - Riedl, Iris
A1 - Weizäcker, Elisabeth von
A1 - Blader, Patrick
A1 - Campos-Ortega, José A.
T1 - her3, a zebrafish member of the hairy-E(spl) family, is repressed by Notch signalling
JF - Development
Y1 - 2004
U6 - https://doi.org/10.1242/dev.01167
SN - 1477-9129
VL - 131
IS - 12
SP - 2957
EP - 2969
ER -
TY - JOUR
A1 - Harish, Ajay B.
A1 - Wriggers, Peter
A1 - Jungk, Juliane
A1 - Hojdis, Nils
A1 - Recker, Carla
T1 - Mesoscale Constitutive Modeling of Non-Crystallizing Filled Elastomers
JF - Computational Mechanics
N2 - Elastomers are exceptional materials owing to their ability to undergo large deformations before failure. However, due to their very low stiffness, they are not always suitable for industrial applications. Addition of filler particles provides reinforcing effects and thus enhances the material properties that render them more versatile for applications like tyres etc. However, deformation behavior of filled polymers is accompanied by several nonlinear effects like Mullins and Payne effect. To this day, the physical and chemical changes resulting in such nonlinear effect remain an active area of research. In this work, we develop a heterogeneous (or multiphase) constitutive model at the mesoscale explicitly considering filler particle aggregates, elastomeric matrix and their mechanical interaction through an approximate interface layer. The developed constitutive model is used to demonstrate cluster breakage, also, as one of the possible sources for Mullins effect observed in non-crystallizing filled elastomers.
Y1 - 2016
U6 - https://doi.org/10.1007/s00466-015-1251-1
SN - 1432-0924
VL - 57
SP - 653
EP - 677
PB - Springer
CY - Berlin
ER -
TY - JOUR
A1 - Hasegawa, Maki
A1 - Kapelyukh, Yury
A1 - Tahara, Harunobu
A1 - Seibler, Jost
A1 - Rode, Anja
A1 - Krueger, Sylvia
A1 - Lee, Dongtao N.
A1 - Wolf, C. Roland
A1 - Scheer, Nico
T1 - Quantitative prediction of human pregnane X receptor and cytochrome P450 3A4 mediated drug-drug interaction in a novel multiple humanized mouse line
JF - Molecular Pharmacology
Y1 - 2011
U6 - https://doi.org/10.1124/mol.111.071845
SN - 1521-0111
VL - 80
IS - 33
SP - 518
EP - 528
PB - ASPET
CY - Bethesda, Md.
ER -
TY - JOUR
A1 - Heine, A.
A1 - Herrmann, G.
A1 - Selmer, Thorsten
A1 - Terwesten, F.
A1 - Buckel, W.
A1 - Reuter, K.
T1 - High resolution crystal structure of clostridium propionicum β-Alanyl-CoA:Ammonia Lyase, a new member of the "Hot Dog Fold" protein superfamily
JF - Proteins
N2 - Clostridium propionicum is the only organism known to ferment β-alanine, a constituent of coenzyme A (CoA) and the phosphopantetheinyl prosthetic group of holo-acyl carrier protein. The first step in the fermentation is a CoA-transfer to β-alanine. Subsequently, the resulting β-alanyl-CoA is deaminated by the enzyme β-alanyl-CoA:ammonia lyase (Acl) to reversibly form ammonia and acrylyl-CoA. We have determined the crystal structure of Acl in its apo-form at a resolution of 0.97 Å as well as in complex with CoA at a resolution of 1.59 Å. The structures reveal that the enyzme belongs to a superfamily of proteins exhibiting a so called “hot dog fold” which is characterized by a five-stranded antiparallel β-sheet with a long α-helix packed against it. The functional unit of all “hot dog fold” proteins is a homodimer containing two equivalent substrate binding sites which are established by the dimer interface. In the case of Acl, three functional dimers combine to a homohexamer strongly resembling the homohexamer formed by YciA-like acyl-CoA thioesterases. Here, we propose an enzymatic mechanism based on the crystal structure of the Acl·CoA complex and molecular docking. Proteins 2014; 82:2041–2053. © 2014 Wiley Periodicals, Inc.
Y1 - 2014
U6 - https://doi.org/10.1002/prot.24557
SN - 1097-0134 (E-Journal); 0887-3585 (Print)
VL - 82
IS - 9
SP - 2041
EP - 2053
PB - Wiley-Liss
CY - New York
ER -
TY - JOUR
A1 - Heinze, D.
A1 - Mang, Thomas
A1 - Popescu, C.
A1 - Weichold, O.
T1 - Effect of side chain length and degree of polymerization on the decomposition and crystallization behaviour of chlorinated poly(vinyl ester) oligomers
JF - Thermochimica Acta
N2 - Four members of a homologous series of chlorinated poly(vinyl ester) oligomers CCl₃–(CH₂CH (OCO(CH₂)ₘCH₃))ₙ–Cl with degrees of polymerization of 10 and 20 were prepared by telomerisation using carbon tetrachloride. The number of side chain carbon atoms ranges from 2 (poly(vinyl acetate) to 18 (poly(vinyl stearate)). The effect of the n-alkyl side chain length and of the degree of polymerization on the thermal stability and crystallization behaviour of the synthesized compounds was investigated.
All oligomers degrade in two major steps by first losing HCl and side chains with subsequent breakdown of the backbone. The members with short side chains, up to poly(vinyl octanoate), are amorphous and show internal plasticization, whereas those with high number of side chain carbon atoms are semi-crystalline due to side-chain crystallization. A better packing for poly(vinyl stearate) is also noticeable. The glass transition and melting temperatures as well as the onset temperature of decomposition are influenced to a larger extent by the side chain length than by the degree of polymerization. Thermal stability is improved if both the size and number of side chains increase, but only a long side chain causes a significant increase of the resistance to degradation. This results in a stabilization of PVAc so that oligomers from poly(vinyl octanoate) on are stable under atmospheric conditions. Thus, the way to design stable, chlorinated PVEs oligomers is to use a long n-alkyl side chain.
Y1 - 2016
U6 - https://doi.org/10.1016/j.tca.2016.05.015
SN - 0040-6031 (electronic)
VL - 637
SP - 143
EP - 153
PB - Elsevier
CY - Amsterdam
ER -
TY - JOUR
A1 - Heinze, Daniel
A1 - Mang, Thomas
A1 - Peter, Karin
A1 - Möller, Martin
A1 - Weichold, Oliver
T1 - Synthesis of low molecular weight poly(vinyl acetate) and its application as plasticizer
JF - Journal of applied polymer science
N2 - Poly(vinyl acetate), PVAc, with a degree of polymerization Xn = 10 was prepared by chain-transfer radical polymerization using carbon tetrachloride and used as oligomeric plasticizer for commercial PVAc. However, the chlorinated chain ends cause a low thermal stability requiring mild Cl/H substitution. The product exhibits high thermal stability and excellent melt-compounding properties. Blends of oligomeric and commercial PVAc show single glass transition temperatures which decrease with higher oligomer content and exhibit small negative deviations from Fox' linear additivity rule. This indicates plasticization and miscibility being mainly due to entropic effects. Injection-moulded thick specimens show ductile behaviour at oligomer contents >10 wt %, while sheets with a thickness of 0.2–0.5 mm appear flexible already at 7.5 wt %. The oxygen permeability coefficients are an order of magnitude lower than those of low-density polyethylene. Due to the sum of their properties, the plasticized sheets present a promising alternative in the preparation of barrier materials.
Y1 - 2014
U6 - https://doi.org/10.1002/app.40226
SN - 1097-4628 (E-Journal); 0021-8995 (Print)
VL - 131
IS - 9
SP - Article No. 40226
PB - Wiley
CY - New York
ER -
TY - JOUR
A1 - Hemmerling, H.-J.
A1 - Merschenz-Quack, Angelika
A1 - Wunderlich, H.
T1 - Crystal structures of indeno[1,2-d]imidazoles. XIth European Crystallographic Meeting, Vienna 1988
JF - Zeitschrift für Kristallographie - Crystalline Materials
Y1 - 1988
SN - 2196-7105 (E-Books); 2194-4946 (Print)
VL - 185
IS - H. 1-4
SP - 256
ER -