TY - JOUR A1 - Akimbekov, Nuraly Shardarbekovich A1 - Mansurov, Zulkhair A1 - Jandosov, J. A1 - Digel, Ilya A1 - Gossmann, Matthias A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül A1 - Zhubanova, Azhar A. T1 - Wound healing activity of carbonized rice husk N2 - The carbonized rice husk (CRH) was evaluated for its wound healing activity in rats using excision models. In this study, the influences of CRH on wound healing in rat skin in vivo and cellular behavior of human dermal fibroblasts in vitro were investigated. The obtained results showed that the CRH treatment promoted wound epithelization in rats and exhibited moderate inhibition of cell proliferation in vitro. CRH with lanolin oil treated wounds were found to epithelize faster as compared to controls. KW - Wundheilung KW - Epithel KW - Fibroblast KW - carbonized rice husk KW - wound healing KW - epithelization KW - human dermal fibroblasts Y1 - 2013 PB - Trans Tech Publications, Switzerland CY - Bäch ER - TY - JOUR A1 - Akimbekov, Nuraly S. A1 - Digel, Ilya A1 - Sherelkhan, Dinara K. A1 - Lutfor, Afzalunnessa B. A1 - Razzaque, Mohammed S. T1 - Vitamin D and the Host-Gut Microbiome: A Brief Overview JF - Acta Histochemica et Cytochemica N2 - There is a growing body of evidence for the effects of vitamin D on intestinal host-microbiome interactions related to gut dysbiosis and bowel inflammation. This brief review highlights the potential links between vitamin D and gut health, emphasizing the role of vitamin D in microbiological and immunological mechanisms of inflammatory bowel diseases. A comprehensive literature search was carried out in PubMed and Google Scholar using combinations of keywords “vitamin D,” “intestines,” “gut microflora,” “bowel inflammation”. Only articles published in English and related to the study topic are included in the review. We discuss how vitamin D (a) modulates intestinal microbiome function, (b) controls antimicrobial peptide expression, and (c) has a protective effect on epithelial barriers in the gut mucosa. Vitamin D and its nuclear receptor (VDR) regulate intestinal barrier integrity, and control innate and adaptive immunity in the gut. Metabolites from the gut microbiota may also regulate expression of VDR, while vitamin D may influence the gut microbiota and exert anti-inflammatory and immune-modulating effects. The underlying mechanism of vitamin D in the pathogenesis of bowel diseases is not fully understood, but maintaining an optimal vitamin D status appears to be beneficial for gut health. Future studies will shed light on the molecular mechanisms through which vitamin D and VDR interactions affect intestinal mucosal immunity, pathogen invasion, symbiont colonization, and antimicrobial peptide expression. Y1 - 2020 U6 - http://dx.doi.org/10.1267/ahc.20011 SN - 1347-5800 VL - 53 IS - 3 SP - 33 EP - 42 PB - Japan Society of Histochemistry and Cytochemistry CY - Osaka ER - TY - JOUR A1 - Akimbekov, N. Sh. A1 - Zhubanova, A. A. A1 - Mansurov, Z. A. A1 - Digel, Ilya A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül T1 - Use of Carbonized Rise Shell for the local treatment of wounds JF - Eurasian ChemTech Journal N2 - On the model of musculocutaneous wound in rats, the effect of applicative sorption by carbonized rise shell (CRS) on the healing of festering wound was studied. It has been shown, that cytological changes end with rapid scar formation. The use of CRS at the period of severe purulent wound contributes to its favorable course, prevents the development of complications of the animals from sepsis. Y1 - 2010 U6 - http://dx.doi.org/10.18321/ectj35 SN - 2522-4867 VL - 12 IS - 2 SP - 133 EP - 138 PB - Institute of Combustion Problems CY - Almaty ER - TY - JOUR A1 - Digel, Ilya A1 - Akimbekov, N. A1 - Turalieva, M. A1 - Mansurov, Z. A1 - Temiz Artmann, Aysegül A1 - Eshibaev, A. A1 - Zhubanova, A. T1 - Usage of Carbonized Plant Wastes for Purification of Aqueous Solutions JF - Journal of Industrial Technology and Engineering Y1 - 2013 VL - 2 IS - 07 SP - 47 EP - 54 ER - TY - JOUR A1 - Seifarth, Volker A1 - Schehl, D. A1 - Linder, Peter A1 - Gossmann, Matthias A1 - Digel, Ilya A1 - Artmann, Gerhard A1 - Porst, Dariusz A1 - Preiß, C. A1 - Kayser, Peter A1 - Pack, O. A1 - Temiz Artmann, Aysegül T1 - Ureplace: development of a bioreactor for in vitro culturing of cell seeded tubular vessels on collagen scaffolds N2 - The demand of replacements for inoperable organs exceeds the amount of available organ transplants. Therefore, tissue engineering developed as a multidisciplinary field of research for autologous in-vitro organs. Such three dimensional tissue constructs request the application of a bioreactor. The UREPLACE bioreactor is used to grow cells on tubular collagen scaffolds OPTIMAIX Sponge 1 with a maximal length of 7 cm, in order to culture in vitro an adequate ureter replacement. With a rotating unit, (urothelial) cells can be placed homogeneously on the inner scaffold surface. Furthermore, a stimulation is combined with this bioreactor resulting in an orientation of muscle cells. These culturing methods request a precise control of several parameters and actuators. A combination of a LabBox and the suitable software LabVision is used to set and conduct parameters like rotation angles, velocities, pressures and other important cell culture values. The bioreactor was tested waterproof successfully. Furthermore, the temperature controlling was adjusted to 37 °C and the CO2 - concentration regulated to 5 %. Additionally, the pH step responses of several substances showed a perfect functioning of the designed flow chamber. All used software was tested and remained stable for several days. KW - Tissue Engineering KW - Bioreaktor KW - Organkultur KW - Harnleiter Y1 - 2011 ER - TY - JOUR A1 - Stadler, Andreas M. A1 - Garvey, G. J. A1 - Bocahut, A. A1 - Sacquin-Mora, S. A1 - Digel, Ilya A1 - Schneider, G. J. A1 - Natali, F. A1 - Artmann, Gerhard A1 - Zaccai, G. T1 - Thermal fluctuations of haemoglobin from different species : adaptation to temperature via conformational dynamics JF - Journal of the Royal Society Interface N2 - Thermodynamic stability, configurational motions and internal forces of haemoglobin (Hb) of three endotherms (platypus, Ornithorhynchus anatinus; domestic chicken, Gallus gallus domesticus and human, Homo sapiens) and an ectotherm (salt water crocodile, Crocodylus porosus) were investigated using circular dichroism, incoherent elastic neutron scattering and coarse-grained Brownian dynamics simulations. The experimental results from Hb solutions revealed a direct correlation between protein resilience, melting temperature and average body temperature of the different species on the 0.1 ns time scale. Molecular forces appeared to be adapted to permit conformational fluctuations with a root mean square displacement close to 1.2 Å at the corresponding average body temperature of the endotherms. Strong forces within crocodile Hb maintain the amplitudes of motion within a narrow limit over the entire temperature range in which the animal lives. In fully hydrated powder samples of human and chicken, Hb mean square displacements and effective force constants on the 1 ns time scale showed no differences over the whole temperature range from 10 to 300 K, in contrast to the solution case. A complementary result of the study, therefore, is that one hydration layer is not sufficient to activate all conformational fluctuations of Hb in the pico- to nanosecond time scale which might be relevant for biological function. Coarse-grained Brownian dynamics simulations permitted to explore residue-specific effects. They indicated that temperature sensing of human and chicken Hb occurs mainly at residues lining internal cavities in the β-subunits. Y1 - 2012 U6 - http://dx.doi.org/10.1098/rsif.2012.0364 SN - 1742-5689 VL - 9 IS - 76 SP - 2845 EP - 2855 PB - The Royal Society CY - London ER - TY - JOUR A1 - Zhubanova, Azhar A. A1 - Digel, Ilya A1 - Nojima, H. A1 - Artmann, Gerhard T1 - The use of bactericidal effects of cluster ions generated by plasma in medical biotechnology N2 - The most of conventional methods of air purification use the power of a fan to draw in air and pass it through a filter. The problem of bacterial contamination of inner parts of such a type of air conditioners in some cases draws attention towards alternative air-cleaning systems. Some manufacturers offer to use the ozone's bactericidal and deodorizing effects, but the wide spreading of such systems is restricted by the fact that toxic effects of ozone in respect of human beings are well known. In 2000 Sharp Inc. introduced "Plasma Cluster Ions (PCI)" air purification technology, which uses plasma discharge to generate cluster ions (I 0-14 ). This technology has been developed for those customers that are conscious about health and hygiene. In our experiments, we focused on some principal aspects of plasma-generated ions application - time-dependency and irreversibility of bactericidal action, spatial and kinetic characteristics of emitted cluster particles, their chemical targets in the microbial cells. KW - Clusterion KW - Raumluft KW - Luftreiniger KW - Plasmacluster ion technology KW - Air purification Y1 - 2007 ER - TY - JOUR A1 - Digel, Ilya A1 - Zhubanova, Azhar A. A1 - Nojima, H. A1 - Artmann, Gerhard T1 - The use of bactericidal effects of cluster ions generated by plasma in medical biotechnology JF - Biotechnologija : teorija i praktika (2004) Y1 - 2004 SN - 1028-9399 SP - 46 EP - 52 ER - TY - JOUR A1 - Digel, Ilya A1 - Temiz Artmann, Aysegül T1 - The emperor's new body : seeking for a blueprint of limb regeneration in humans JF - Stem cell engineering : principles and applications / Gerhard M. Artmann ... eds. Y1 - 2011 SN - 978-3-642-11864-7 SP - 3 EP - 37 PB - Springer CY - Berlin [u.a.] ER - TY - JOUR A1 - Akimbekov, Nuraly A1 - Qiao, Xiaohui A1 - Digel, Ilya A1 - Abdieva, Gulzhamal A1 - Ualieva, Perizat A1 - Zhubanova, Azhar T1 - The effect of leonardite-derived amendments on soil microbiome structure and potato yield JF - Agriculture N2 - Humic substances originating from various organic matters can ameliorate soil properties, stimulate plant growth, and improve nutrient uptake. Due to the low calorific heating value, leonardite is rather unsuitable as fuel. However, it may serve as a potential source of humic substances. This study was aimed at characterizing the leonardite-based soil amendments and examining the effect of their application on the soil microbial community, as well as on potato growth and tuber yield. A high yield (71.1%) of humic acid (LHA) from leonardite has been demonstrated. Parental leonardite (PL) and LHA were applied to soil prior to potato cultivation. The 16S rRNA sequencing of soil samples revealed distinct relationships between microbial community composition and the application of leonardite-based soil amendments. Potato tubers were planted in pots in greenhouse conditions. The tubers were harvested at the mature stage for the determination of growth and yield parameters. The results demonstrated that the LHA treatments had a significant effect on increasing potato growth (54.9%) and tuber yield (66.4%) when compared to the control. The findings highlight the importance of amending leonardite-based humic products for maintaining the biogeochemical stability of soils, for keeping their healthy microbial community structure, and for increasing the agronomic productivity of potato plants. Y1 - 2020 U6 - http://dx.doi.org/10.3390/agriculture10050147 VL - 10 IS - Art. 147 SP - 1 EP - 17 PB - MDPI CY - Basel ER - TY - JOUR A1 - Turlybekuly, Amanzhol A1 - Pogrebnjak, Alexander A1 - Sukhodub, L. F. A1 - Sukhodub, Liudmyla B. A1 - Kistaubayeva, A. S. A1 - Savitskaya, Irina A1 - Shokatayeva, D. H. A1 - Bondar, Oleksandr V. A1 - Shaimardanov, Z. K. A1 - Plotnikov, Sergey V. A1 - Shaimardanova, B. H. A1 - Digel, Ilya T1 - Synthesis, characterization, in vitro biocompatibility and antibacterial properties study of nanocomposite materials based on hydroxyapatite-biphasic ZnO micro- and nanoparticles embedded in Alginate matrix JF - Materials Science and Engineering C Y1 - 2019 U6 - http://dx.doi.org/10.1016/j.msec.2019.109965 VL - 104 IS - Article number 109965 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Digel, Ilya A1 - Wehlitz, V. A1 - Kayser, Peter A1 - Figiel-Lange, A. A1 - Bassam, R. A1 - Rundstedt, F. von T1 - Suspension depletion approach for exemption of infected Solanum jasminoides cells from pospiviroids JF - Plant Pathology N2 - Despite numerous studies, viroid elimination from infected plants remains a very challenging task. This study introduces for the first time a novel ‘suspension depletion’ approach for exemption of Solanum jasminoides plants from viroids. The proposed method implies initial establishment of suspension cultures of the infected plant cells. The suspended cells were then physically treated (mild thermotherapy, 33 °C), which presumably delayed the replication of the viroid. The viroid concentration in the treated biomass was monitored weekly using pospiviroid-specific PCR. After 10–12 weeks of continuous treatment, a sufficient decrease in viroid concentration was observed such that the infection became undetectable by PCR. The treated single cells then gave rise to microcolonies on a solid culture medium and the obtained viroid-negative clones were further promoted to regenerate into viroid-free plants. Three years of accumulated experimental data suggests feasibility, broad applicability, and good efficacy of the proposed approach. Y1 - 2018 U6 - http://dx.doi.org/10.1111/ppa.12750 SN - 1365-3059 VL - 67 IS - 2 SP - 358 EP - 365 PB - Wiley CY - Oxford ER - TY - JOUR A1 - Zerlin, Kay A1 - Kasischke, Nicole A1 - Digel, Ilya A1 - Maggakis-Kelemen, Christina A1 - Temiz Artmann, Aysegül A1 - Porst, Dariusz A1 - Kayser, Peter A1 - Linder, Peter A1 - Artmann, Gerhard T1 - Structural transition temperature of hemoglobins correlates with species’ body temperature JF - European Biophysics Journal. 37 (2007), H. 1 Y1 - 2007 SN - 1432-1017 SP - 1 EP - 10 ER - TY - JOUR A1 - Turaliyeva, M. A1 - Yeshibaev, A. A1 - Saparbekova, A. A1 - Akynova, L. A1 - Abildayeva, R. A1 - Sadenova, M. A1 - Sartayeva, K. A1 - Schieffer, Andre A1 - Digel, Ilya T1 - Species composition and injuriousness of stranger xylophilous fauna affecting indigenous urban dendroflora of Central Asia JF - Asian journal of microbiology, biotechnology & environmental sciences : AJMBES N2 - At the present time, one of the most serious environmental problems of Central Asia and South Kazakhstan is the ongoing large-scale deterioration of principal urban tree populations. Several major centers of massive spread of invasive plant pests have been found in urban dendroflora of this region. The degree of damage of seven most wide-spread aboriginal tree species was found to range from 21.4±1.1 to 85.4±1.8%. In particular, the integrity of the native communities of sycamore (Platanus spp.), willow (Salix spp.), poplar (Populus spp.) and elm (Ulmus spp.) is highly endangered. Our taxonomic analysis of the most dangerous tree pests of the region has revealed them as neobiontic xylophilous insects such as Cossus cossus L. (Order: Lepidoptera L.) Monochamus urussovi Fisch., Monochamus sutor L., Acanthocinus aedelis L. and Ñetonia aureate L. (Order: Coleoptera L.). We relate the origin of this threatening trend with the import of industrial wood in the mid 90’s of the last century that was associated with high degree of the constructional work in the region. Because of the absence of efficient natural predators of the pest species, the application of microbiological methods of the pest control and limitation is suggested. Y1 - 2016 SN - 0972-3005 VL - 18 IS - 2 SP - 359 EP - 366 PB - EM International ER - TY - JOUR A1 - Kurulgan Demirci, Eylem A1 - Demirci, Taylan A1 - Linder, Peter A1 - Trzewik, Jürgen A1 - Gierkowski, Jessica Ricarda A1 - Gossmann, Matthias A1 - Kayser, Peter A1 - Porst, Dariusz A1 - Digel, Ilya A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül T1 - rhAPC reduces the endothelial cell permeability via a decrease of contractile tensions induced by endothelial cells JF - Journal of Bioscience and Bioengineering N2 - All cells generate contractile tension. This strain is crucial for mechanically controlling the cell shape, function and survival. In this study, the CellDrum technology quantifying cell's (the cellular) mechanical tension on a pico-scale was used to investigate the effect of lipopolysaccharide (LPS) on human aortic endothelial cell (HAoEC) tension. The LPS effect during gram-negative sepsis on endothelial cells is cell contraction causing endothelium permeability increase. The aim was to finding out whether recombinant activated protein C (rhAPC) would reverse the endothelial cell response in an in-vitro sepsis model. In this study, the established in-vitro sepsis model was confirmed by interleukin 6 (IL-6) levels at the proteomic and genomic levels by ELISA, real time-PCR and reactive oxygen species (ROS) activation by florescence staining. The thrombin cellular contraction effect on endothelial cells was used as a positive control when the CellDrum technology was applied. Additionally, the Ras homolog gene family, member A (RhoA) mRNA expression level was checked by real time-PCR to support contractile tension results. According to contractile tension results, the mechanical predominance of actin stress fibers was a reason of the increased endothelial contractile tension leading to enhanced endothelium contractility and thus permeability enhancement. The originality of this data supports firstly the basic measurement principles of the CellDrum technology and secondly that rhAPC has a beneficial effect on sepsis influenced cellular tension. The technology presented here is promising for future high-throughput cellular tension analysis that will help identify pathological contractile tension responses of cells and prove further cell in-vitro models. KW - Cell permeability KW - Cellular force KW - Endothelial cells KW - Recombinant activated protein C KW - Lipopolysaccharide KW - Contractile tension KW - CellDrum Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.jbiosc.2012.03.019 SN - 1347-4421 VL - 113 IS - 2 SP - 212 EP - 219 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Digel, Ilya A1 - Trzewik, Jürgen A1 - Demirci, Taylan A1 - Temiz Artmann, Aysegül T1 - Response of fibroblasts to cyclic mechanical stress : a proteome approach / Digel, I. ; Trzewik, J. ; Demirci, T. ; Temiz Artmann, A. ; Artmann, G. M. JF - Biomedizinische Technik. 49 (2004), H. Erg.-Bd. 2 Y1 - 2004 SN - 0932-4666 SP - 1042 EP - 1043 ER - TY - JOUR A1 - Digel, Ilya A1 - Zerlin, Kay A1 - Temiz Artmann, Aysegül A1 - Engels, S. T1 - Protein dynamics in thermosensation JF - Regenerative medicine. 2 (2007), H. 5 Y1 - 2007 SN - 1746-0751 N1 - Proceedings of the 3rd World Congress on Regenerative Medicine. October 18-20, 2007. Leipzig, Germany SP - 533 EP - 533 ER - TY - JOUR A1 - Digel, Ilya T1 - Primary thermosensory events in cells JF - Transient receptor potential channels / Md. Shahidul Islam, ed. Y1 - 2011 SN - 978-94-007-0264-6 N1 - (Advances in experimental medicine and biology ; 704) SP - 451 EP - 468 PB - Springer CY - Dordrecht [u.a.] ER - TY - JOUR A1 - Savitskaya, Irina A1 - Zhantlessova, Sirina A1 - Kistaubayeva, Aida A1 - Ignatova, Ludmila A1 - Shokatayeva, Dina A1 - Sinyavsky, Yuriy A1 - Kushugulova, Almagul A1 - Digel, Ilya T1 - Prebiotic cellulose–pullulan matrix as a “vehicle” for probiotic biofilm delivery to the host large intestine JF - Polymers N2 - This study describes the development of a new combined polysaccharide-matrix-based technology for the immobilization of Lactobacillus rhamnosus GG (LGG) bacteria in biofilm form. The new composition allows for delivering the bacteria to the digestive tract in a manner that improves their robustness compared with planktonic cells and released biofilm cells. Granules consisting of a polysaccharide matrix with probiotic biofilms (PMPB) with high cell density (>9 log CFU/g) were obtained by immobilization in the optimized nutrient medium. Successful probiotic loading was confirmed by fluorescence microscopy and scanning electron microscopy. The developed prebiotic polysaccharide matrix significantly enhanced LGG viability under acidic (pH 2.0) and bile salt (0.3%) stress conditions. Enzymatic extract of feces, mimicking colon fluid in terms of cellulase activity, was used to evaluate the intestinal release of probiotics. PMPB granules showed the ability to gradually release a large number of viable LGG cells in the model colon fluid. In vivo, the oral administration of PMPB granules in rats resulted in the successful release of probiotics in the colon environment. The biofilm-forming incubation method of immobilization on a complex polysaccharide matrix tested in this study has shown high efficacy and promising potential for the development of innovative biotechnologies. KW - immobilization KW - prebiotic KW - bacterial cellulose KW - biofilms KW - Lactobacillus rhamnosus GG Y1 - 2023 U6 - http://dx.doi.org/10.3390/polym16010030 N1 - This article belongs to the Section "Polymer Composites and Nanocomposites" IS - 16(1) PB - MDPI CY - Basel ER - TY - JOUR A1 - Maggakis-Kelemen, Christina A1 - Digel, Ilya A1 - Artmann, Gerhard T1 - Polystyrene sulfonate/Polyallylamine hydrochloride microcapsules as potential artificial red blood cells - improvement of capsule flexibility JF - Biomedizinische Technik. 50 (2005), H. Erg.-Bd. 1 Y1 - 2005 SP - 324 EP - 326 ER - TY - JOUR A1 - Savitskaya, I. S. A1 - Kistaubayeva, A. S. A1 - Digel, Ilya A1 - Shokatayeva, D. H. T1 - Physicochemical and Antibacterial Properties of Composite Films Based on Bacterial Cellulose and Chitosan for Wound Dressing Materials JF - Eurasian Chemico-Technological Journal Y1 - 2017 U6 - http://dx.doi.org/10.18321/ectj670 SN - 2522-4867 VL - 19 IS - 3 SP - 255 EP - 264 ER - TY - JOUR A1 - Artmann, Gerhard A1 - Zerlin, Kay A1 - Digel, Ilya A1 - Stadler, Andreas M. T1 - Physical discontinuities at body temperature in human red blood cells / Artmann, GM ; Zerlin, K ; Digel, I ; Stadler, A ; Zaccai, G ; Temiz, AA JF - Tissue Engineering. 13 (2007), H. 7 Y1 - 2007 SN - 1076-3279 N1 - TERMIS-EU Meeting Abstracts London, UK September 4–7, 2007 SP - 1778 EP - 1778 ER - TY - JOUR A1 - Arinkin, Vladimir A1 - Digel, Ilya A1 - Porst, Dariusz A1 - Temiz Artmann, Aysegül A1 - Artmann, Gerhard T1 - Phenotyping date palm varieties via leaflet cross-sectional imaging and artificial neural network application JF - BMC bioinformatics N2 - Background True date palms (Phoenix dactylifera L.) are impressive trees and have served as an indispensable source of food for mankind in tropical and subtropical countries for centuries. The aim of this study is to differentiate date palm tree varieties by analysing leaflet cross sections with technical/optical methods and artificial neural networks (ANN). Results Fluorescence microscopy images of leaflet cross sections have been taken from a set of five date palm tree cultivars (Hewlat al Jouf, Khlas, Nabot Soltan, Shishi, Um Raheem). After features extraction from images, the obtained data have been fed in a multilayer perceptron ANN with backpropagation learning algorithm. Conclusions Overall, an accurate result in prediction and differentiation of date palm tree cultivars was achieved with average prediction in tenfold cross-validation is 89.1% and reached 100% in one of the best ANN. Y1 - 2014 U6 - http://dx.doi.org/10.1186/1471-2105-15-55 SN - 1471-2105 VL - 15 IS - 55 SP - 1 EP - 8 ER - TY - JOUR A1 - Sadykov, Rustam A1 - Digel, Ilya A1 - Temiz Artmann, Aysegül A1 - Porst, Dariusz A1 - Linder, Peter A1 - Kayser, Peter A1 - Artmann, Gerhard A1 - Savitskaya, Irina A1 - Zhubanova, Azhar T1 - Oral lead exposure induces dysbacteriosis in rats JF - Journal of Occupational Health. 51 (2009) (2009), H. 1 Y1 - 2009 SN - 1348-9585 SP - 64 EP - 73 ER - TY - JOUR A1 - Micili, Serap C. A1 - Valter, Markus A1 - Oflaz, Hakan A1 - Ozogul, Candan A1 - Linder, Peter A1 - Föckler, Nicole A1 - Artmann, Gerhard A1 - Digel, Ilya A1 - Temiz Artmann, Aysegül T1 - Optical coherence tomography : a potential tool to predict premature rupture of fetal membranes JF - Proceedings of the Institution of Mechanical Engineers. Part H : Journal of engineering in medicine Y1 - 2013 SN - 0046-2039 (Print) ; 2041-3033 (E-Journal) VL - Vol. 227 IS - No. 4 SP - 393 EP - 401 PB - Sage CY - London ER - TY - JOUR A1 - Temiz Artmann, Aysegül A1 - Linder, Peter A1 - Kayser, Peter A1 - Digel, Ilya T1 - NMR in vitro effects on proliferation, apoptosis, and viability of human chondrocytes and osteoblasts JF - Methods and findings in Experimental and Clinical Pharmacology. 27 (2005), H. 6 Y1 - 2005 SN - 0379-0355 SP - 391 EP - 394 ER - TY - JOUR A1 - Kowalski, Julia A1 - Linder, Peter A1 - Zierke, S. A1 - Wulfen, B. van A1 - Clemens, J. A1 - Konstantinidis, K. A1 - Ameres, G. A1 - Hoffmann, R. A1 - Mikucki, J. A1 - Tulaczyk, S. A1 - Funke, O. A1 - Blandfort, D. A1 - Espe, Clemens A1 - Feldmann, Marco A1 - Francke, Gero A1 - Hiecker, S. A1 - Plescher, Engelbert A1 - Schöngarth, Sarah A1 - Dachwald, Bernd A1 - Digel, Ilya A1 - Artmann, Gerhard A1 - Eliseev, D. A1 - Heinen, D. A1 - Scholz, F. A1 - Wiebusch, C. A1 - Macht, S. A1 - Bestmann, U. A1 - Reineking, T. A1 - Zetzsche, C. A1 - Schill, K. A1 - Förstner, R. A1 - Niedermeier, H. A1 - Szumski, A. A1 - Eissfeller, B. A1 - Naumann, U. A1 - Helbing, K. T1 - Navigation technology for exploration of glacier ice with maneuverable melting probes JF - Cold Regions Science and Technology N2 - The Saturnian moon Enceladus with its extensive water bodies underneath a thick ice sheet cover is a potential candidate for extraterrestrial life. Direct exploration of such extraterrestrial aquatic ecosystems requires advanced access and sampling technologies with a high level of autonomy. A new technological approach has been developed as part of the collaborative research project Enceladus Explorer (EnEx). The concept is based upon a minimally invasive melting probe called the IceMole. The force-regulated, heater-controlled IceMole is able to travel along a curved trajectory as well as upwards. Hence, it allows maneuvers which may be necessary for obstacle avoidance or target selection. Maneuverability, however, necessitates a sophisticated on-board navigation system capable of autonomous operations. The development of such a navigational system has been the focal part of the EnEx project. The original IceMole has been further developed to include relative positioning based on in-ice attitude determination, acoustic positioning, ultrasonic obstacle and target detection integrated through a high-level sensor fusion. This paper describes the EnEx technology and discusses implications for an actual extraterrestrial mission concept. Y1 - 2016 U6 - http://dx.doi.org/10.1016/j.coldregions.2015.11.006 SN - 0165-232X IS - 123 SP - 53 EP - 70 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Mansurov, Z. A. A1 - Jandosov, J. M. A1 - Kerimkulova, A. R. A1 - Azat, S. A1 - Zhubanova, A. A. A1 - Digel, Ilya A1 - Savistkaya, I. S. A1 - Akimbekov, N. S. A1 - Kistaubaeva, A. S. T1 - Nanostructured carbon materials for biomedical use JF - Eurasian chemico-technological journal : quarterly journal of the International Higher Education Academy of Sciences N2 - One of the priority trends of carbon nanotechnology is creation of nanocomposite systems. Such carbon nanostructured composites were produced using - raw materials based on the products of agricultural waste, such as grape stones, apricot stones, rice husk. These products have a - wide spectrum of application and can be obtained in large quantities. The Institute of Combustion Problems has carried out the work on synthesis of the nanostructured carbon sorbents for multiple applications including the field of biomedicine. The article presents the data on the synthesis and physico-chemical properties of carbonaceous sorbents using physicochemical methods of investigation: separation and purification of biomolecules; isolation of phytohormone - fusicoccin; adsorbent INGO-1 in the form of an adsorption column for blood detoxification, oral (entero) sorbent - INGO-2; the study of efferent and probiotic properties and sorption activity in regard to the lipopolysaccharide (LPS), new biocomposites - based on carbonized rice husk (CRH) and cellular microorganisms; the use of CRH in wound treatment. A new material for blood detoxication (INGO-1) has been obtained. Adsorption of p-cresyl sulfate and indoxyl sulfate has shown that active carbon adsorbent can remove clinically significant level of p-cresyl sulfate and indoxyl sulfate from human plasma. Enterosorbent INGO-2 possesses high adsorption activity in relation to Gram-negative bacteria and their endotoxins. INGO-2 slows down the growth of conditionally pathogenic microorganisms, without having a negative effect on bifido and lactobacteria. The use of enterosorbent INGO-2 for sorption therapy may provide a solution to a complex problem - detoxication of the digestive tract and normalization of the intestinal micro ecology. The immobilized probiotic called "Riso-lact" was registered at the Ministry of Health of the Republic of Kazakhstan as a biologically active food additive. The developed technology is patented and provides production of the medicine in the form of freeze-dried biomass immobilized in vials. Y1 - 2014 U6 - http://dx.doi.org/10.18321/ectj224 SN - 1562-3920 VL - 15 (2013) IS - 3 SP - 209 EP - 217 PB - Institute of Combustion Problems CY - Almaty ER - TY - JOUR A1 - Turaliyeva, M. A1 - Yeshibaev, A. A1 - Digel, Ilya A1 - Elibayeva, G. A1 - Sydykova, A. A1 - Uspabayeva, A. A1 - Dosybayeva, G. A1 - Zhylysbayeva, A. A1 - Lakhanova, K. T1 - Molecular-genetic identification of emerged novel invasive pathogens of Asiatic Elm Ulmus pumila L JF - Life science journal N2 - The dwarf elm Ulmus pumila L. (Ulmaceae) is one of indigenous species of flora in Kazakhstan and forms a basis of dendroflora in virtually all settlements of the region. In the past decade, multiple outbreaks of previously unknown diseases of the small-leaved elm have been registered. In our study, by the molecular-genetic analysis it was found that the pathogens responsible for the outbreaks are microfungi belonging to the genus Fusarium – F. solani and F. oxysporum. The nucleotide sequences (ITS regions) isolated from the diseased trees showed very high similarity with the GenBank control numbers EU625403.1 and FJ478128.1 (100.0 and 99.0 % respectively). Oncoming research will focus on the search of natural microbial antagonists of the discovered phytopathogens. Y1 - 2014 U6 - http://dx.doi.org/10.7537/marslsj1105s14.33 SN - 1097-8135 VL - 11 IS - Spec. iss. 5s SP - 171 EP - 175 PB - Marsland Press CY - New York ER - TY - JOUR A1 - Digel, Ilya A1 - Kayser, Peter A1 - Artmann, Gerhard T1 - Molecular processes in biological thermosensation JF - Journal of Biophysics. 2008 (2008) Y1 - 2008 SN - 1687-8000 SP - 1 EP - 9 ER - TY - JOUR A1 - Sagymbay, Altynay A1 - G.E., Nusupbaeva A1 - N.Zh, Tleumbetova A1 - A.S., Mutalieva A1 - Nurpeisova, Ainur A1 - D.B., Jussupova A1 - Digel, Ilya T1 - Molecular genetics features of the epidemic season 2017-2018 on the influenza in Kazakhstan JF - Eurasian Journal of Ecology Y1 - 2019 SN - 2617-7358 VL - 58 IS - 1 SP - 50 EP - 60 ER - TY - JOUR A1 - Bayer, Robin A1 - Temiz Artmann, Aysegül A1 - Digel, Ilya A1 - Falkenstein, Julia A1 - Artmann, Gerhard A1 - Creutz, Till A1 - Hescheler, Jürgen T1 - Mechano-pharmacological testing of L-Type Ca²⁺ channel modulators via human vascular celldrum model JF - Cellular Physiology and Biochemistry N2 - Background/Aims: This study aimed to establish a precise and well-defined working model, assessing pharmaceutical effects on vascular smooth muscle cell monolayer in-vitro. It describes various analysis techniques to determine the most suitable to measure the biomechanical impact of vasoactive agents by using CellDrum technology. Methods: The so-called CellDrum technology was applied to analyse the biomechanical properties of confluent human aorta muscle cells (haSMC) in monolayer. The cell generated tensions deviations in the range of a few N/m² are evaluated by the CellDrum technology. This study focuses on the dilative and contractive effects of L-type Ca²⁺ channel agonists and antagonists, respectively. We analyzed the effects of Bay K8644, nifedipine and verapamil. Three different measurement modes were developed and applied to determine the most appropriate analysis technique for the study purpose. These three operation modes are called, particular time mode" (PTM), "long term mode" (LTM) and "real-time mode" (RTM). Results: It was possible to quantify the biomechanical response of haSMCs to the addition of vasoactive agents using CellDrum technology. Due to the supplementation of 100nM Bay K8644, the tension increased approximately 10.6% from initial tension maximum, whereas, the treatment with nifedipine and verapamil caused a significant decrease in cellular tension: 10nM nifedipine decreased the biomechanical stress around 6,5% and 50nM verapamil by 2,8%, compared to the initial tension maximum. Additionally, all tested measurement modes provide similar results while focusing on different analysis parameters. Conclusion: The CellDrum technology allows highly sensitive biomechanical stress measurements of cultured haSMC monolayers. The mechanical stress responses evoked by the application of vasoactive calcium channel modulators were quantified functionally (N/m²). All tested operation modes resulted in equal findings, whereas each mode features operation-related data analysis. Y1 - 2020 U6 - http://dx.doi.org/10.33594/000000225 SN - 1421-9778 VL - 54 SP - 371 EP - 383 PB - Cell Physiol Biochem Press CY - Düsseldorf ER - TY - JOUR A1 - Artmann, Gerhard A1 - Digel, Ilya A1 - Linder, Peter A1 - Porst, Dariusz T1 - Mechanism of haemoglobin sensing body temperature JF - Tissue Engineering Part A. 14 (2008), H. 5 Y1 - 2008 SN - 1937-3341 N1 - TERMIS EU 2008 Porto Meeting June 22–26, 2008 Porto Congress Center–Alfândega Portugal SP - 754 EP - 754 ER - TY - JOUR A1 - Demirci, T. A1 - Trzewik, J. A1 - Linder, Peter A1 - Digel, Ilya A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül T1 - Mechanical Stimulation of 3T3 Fibroblasts Activates Genes: ITGB5 and p53 Responses as Quantified on the mRNA Level JF - Biomedizinische Technik . 49 (2004), H. Erg.-Bd. 2 Y1 - 2004 SN - 0932-4666 SP - 1030 EP - 1031 ER - TY - JOUR A1 - Akimbekov, Nuraly S. A1 - Digel, Ilya A1 - Tastambek, Kuanysh T. A1 - Sherelkhan, Dinara K. A1 - Jussupova, Dariya B. A1 - Altynbay, Nazym P. T1 - Low-rank coal as a source of humic substances for soil amendment and fertility management JF - Agriculture N2 - Humic substances (HS), as important environmental components, are essential to soil health and agricultural sustainability. The usage of low-rank coal (LRC) for energy generation has declined considerably due to the growing popularity of renewable energy sources and gas. However, their potential as soil amendment aimed to maintain soil quality and productivity deserves more recognition. LRC, a highly heterogeneous material in nature, contains large quantities of HS and may effectively help to restore the physicochemical, biological, and ecological functionality of soil. Multiple emerging studies support the view that LRC and its derivatives can positively impact the soil microclimate, nutrient status, and organic matter turnover. Moreover, the phytotoxic effects of some pollutants can be reduced by subsequent LRC application. Broad geographical availability, relatively low cost, and good technical applicability of LRC offer the advantage of easy fulfilling soil amendment and conditioner requirements worldwide. This review analyzes and emphasizes the potential of LRC and its numerous forms/combinations for soil amelioration and crop production. A great benefit would be a systematic investment strategy implicating safe utilization and long-term application of LRC for sustainable agricultural production. KW - soil remediation KW - crop yield KW - soil health KW - soil amendment KW - low-rank coal Y1 - 2021 U6 - http://dx.doi.org/10.3390/agriculture11121261 SN - 2077-0472 N1 - This article belongs to the Special Issue "From Waste to Fertilizer in Sustainable Agriculture" VL - 11 IS - 12 PB - MDPI CY - Basel ER - TY - JOUR A1 - Akimbekov, Nuraly A1 - Digel, Ilya A1 - Abdieva, Gulzhamal A1 - Ualieva, Perizat A1 - Tastambek, Kuanysh T1 - Lignite biosolubilization and bioconversion by Bacillus sp.: the collation of analytical data JF - Biofuels N2 - The vast metabolic potential of microbes in brown coal (lignite) processing and utilization can greatly contribute to innovative approaches to sustainable production of high-value products from coal. In this study, the multi-faceted and complex coal biosolubilization process by Bacillus sp. RKB 7 isolate from the Kazakhstan coal-mining soil is reported, and the derived products are characterized. Lignite solubilization tests performed for surface and suspension cultures testify to the formation of numerous soluble lignite-derived substances. Almost 24% of crude lignite (5% w/v) was solubilized within 14 days under slightly alkaline conditions (pH 8.2). FTIR analysis revealed various functional groups in the obtained biosolubilization products. Analyses of the lignite-derived humic products by UV-Vis and fluorescence spectrometry as well as elemental analysis yielded compatible results indicating the emerging products had a lower molecular weight and degree of aromaticity. Furthermore, XRD and SEM analyses were used to evaluate the biosolubilization processes from mineralogical and microscopic points of view. The findings not only contribute to a deeper understanding of microbe–mineral interactions in coal environments, but also contribute to knowledge of coal biosolubilization and bioconversion with regard to sustainable production of humic substances. The detailed and comprehensive analyses demonstrate the huge biotechnological potential of Bacillus sp. for agricultural productivity and environmental health. KW - humic acid KW - Bacillus sp KW - lignite KW - Biosolubilization Y1 - 2021 SN - 1759-7277 VL - 12 IS - 3 SP - 247 EP - 258 PB - Taylor & Francis CY - London ER - TY - JOUR A1 - Akimbekov, N.Sh. A1 - Digel, Ilya A1 - Savitkaya, I.S. A1 - Zhubanova, A.A. A1 - Tastambek, K.T. T1 - Investigations of LPS endotoxin elimination in the flowing column conditions with the sorbent on the basis of carbonized rice husk JF - KazNU Bulletin. Biology series Y1 - 2013 SN - 1563-0218 N1 - Original in russischer Sprache VL - 57 IS - 1 SP - 124 EP - 127 ER - TY - JOUR A1 - Akimbekov, N. Sh. A1 - Digel, Ilya A1 - Zhubanova, A. A. T1 - Investigation the Influence of Carbonized Material Based On Rice Husk on Viability and Migration of Fibroblasts in T3B3 Cell Culture JF - KazNU Bulletin. Biology series Y1 - 2013 SN - 1563-0218 N1 - Original in russischer Sprache VL - 59 IS - 3/1 SP - 20 EP - 23 ER - TY - JOUR A1 - Digel, Ilya T1 - In-situ biological decontamination of an ice melting probe Y1 - 2010 N1 - 38th COSPAR Scientific Assembly. Held 18-15 July 2010, in Bremen, Germany Abstract unter https://www.cospar-assembly.org/abstractcd/OLD/COSPAR-10/abstracts/data/pdf/abstracts/F36-0013-10.pdf ER - TY - JOUR A1 - Bogoyavlenskiy, A. P. A1 - Berezin, V. E. A1 - Ogneva, A. V. A1 - Tolmacheva, V. P. A1 - Digel, Ilya A1 - Khudyakova, S. S. T1 - Immunostimulating activity of a saponin-containing extract of Saponaria officinalis JF - Voprosy virusologii Y1 - 1999 SN - 0507-4088 VL - 44 IS - 5 SP - 229 EP - 232 ER - TY - JOUR A1 - Dachwald, Bernd A1 - Mikucki, Jill A1 - Tulaczyk, Slawek A1 - Digel, Ilya A1 - Espe, Clemens A1 - Feldmann, Marco A1 - Francke, Gero A1 - Kowalski, Julia A1 - Xu, Changsheng T1 - IceMole : A maneuverable probe for clean in situ analysis and sampling of subsurface ice and subglacial aquatic ecosystems JF - Annals of Glaciology N2 - There is significant interest in sampling subglacial environments for geobiological studies, but they are difficult to access. Existing ice-drilling technologies make it cumbersome to maintain microbiologically clean access for sample acquisition and environmental stewardship of potentially fragile subglacial aquatic ecosystems. The IceMole is a maneuverable subsurface ice probe for clean in situ analysis and sampling of glacial ice and subglacial materials. The design is based on the novel concept of combining melting and mechanical propulsion. It can change melting direction by differential heating of the melting head and optional side-wall heaters. The first two prototypes were successfully tested between 2010 and 2012 on glaciers in Switzerland and Iceland. They demonstrated downward, horizontal and upward melting, as well as curve driving and dirt layer penetration. A more advanced probe is currently under development as part of the Enceladus Explorer (EnEx) project. It offers systems for obstacle avoidance, target detection, and navigation in ice. For the EnEx-IceMole, we will pay particular attention to clean protocols for the sampling of subglacial materials for biogeochemical analysis. We plan to use this probe for clean access into a unique subglacial aquatic environment at Blood Falls, Antarctica, with return of a subglacial brine sample. KW - Antarctic Glaciology KW - Extraterrestrial Glaciology KW - Glaciological instruments and methods KW - Subclacial exploration KW - Subglacial lakes Y1 - 2014 U6 - http://dx.doi.org/10.3189/2014AoG65A004 SN - 1727-5644 VL - 55 IS - 65 SP - 14 EP - 22 PB - Cambridge University Press CY - Cambridge ER - TY - JOUR A1 - Linder, Peter A1 - Digel, Ilya A1 - Temiz Artmann, Aysegül A1 - Kayser, Peter A1 - Porst, Dariusz A1 - Artmann, Gerhard T1 - High-throughput testing of mechanical forces generated in thin cell and tissue layers JF - Tissue Engineering. 13 (2007), H. 7 Y1 - 2007 SN - 1076-3279 N1 - Meeting abstract 433 SP - 1778 EP - 1778 ER - TY - JOUR A1 - Artmann, Gerhard A1 - Zerlin, Kay A1 - Digel, Ilya T1 - Hemoglobin Senses Body Temperature JF - Bioengineering in Cell and Tissue Research / Artmann, Gerhard M. ; Chien, Shu (Eds.) Y1 - 2008 SN - 978-3-540-75408-4 SP - 415 EP - 447 PB - Springer CY - Berlin ER - TY - JOUR A1 - Artmann, Gerhard A1 - Digel, Ilya A1 - Zerlin, Kay A1 - Maggakis-Kelemen, Christina A1 - Linder, Peter A1 - Porst, Dariusz A1 - Kayser, Peter A1 - Stadler, David A1 - Dikta, Gerhard A1 - Temiz Artmann, Aysegül T1 - Hemoglobin senses body temperature JF - European Biophysics Journal Y1 - 2009 SN - 0175-7571 VL - 38 IS - 5 SP - 589 EP - 600 ER - TY - JOUR A1 - Stadler, A. M. A1 - Digel, Ilya A1 - Artmann, Gerhard A1 - Embs, Jan P. A1 - Zaccai, Joe A1 - Büldt, Georg T1 - Hemoglobin Dynamics in Red Blood Cells: Correlation to Body Temperature JF - Biophysical Journal. 95 (2008), H. 11 Y1 - 2008 SN - 1542-0086 SP - 5449 EP - 5461 ER - TY - JOUR A1 - Demirci, Taylan A1 - Kurulgan Demirci, Eylem A1 - Trzewik, Jürgen A1 - Linder, Peter A1 - Digel, Ilya A1 - Artmann, Gerhard A1 - Sakizli, Meral A1 - Temiz Artmann, Aysegül T1 - Gene expression profile analysis of 3T3/NIH fibroblasts after one hour mechanical stress JF - IUBMB Life. 61 (2009), H. 3 Y1 - 2009 SN - 1521-6543 N1 - Abstracts: Turkish Society of Molecular Medicine, Third International Congress of Molecular Medicine, May 5-8, 2009, Istanbul, Turkey SP - 311 EP - 312 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Stadler, Andreas M. A1 - Digel, Ilya A1 - Embs, Jan P. A1 - Unruh, Tobias A1 - Tehei, M. A1 - Zaccai, G. A1 - Büldt, G. A1 - Artmann, Gerhard T1 - From powder to solution : Hydration dependence of human hemoglobin dynamics correlated to body temperature JF - Biophysical Journal. 96 (2009), H. 12 Y1 - 2009 SN - 0006-3495 SP - 5073 EP - 5081 PB - Cell Press CY - Cambridge, Mass. ER - TY - JOUR A1 - Digel, Ilya A1 - Demirci, Taylan A1 - Temiz Artmann, Aysegül A1 - Nishikawa, K. T1 - Free Radical Nature of the Bactericidal Effect of Plasma-Generated Cluster Ions (PCIs) JF - Biomedizinische Technik. 49 (2004), H. Erg.-Bd. 2 Y1 - 2004 SN - 0932-4666 SP - 982 EP - 983 ER - TY - JOUR A1 - Leimena, W. A1 - Artmann, Gerhard A1 - Dachwald, Bernd A1 - Temiz Artmann, Aysegül A1 - Gossmann, Matthias A1 - Digel, Ilya T1 - Feasibility of an in-situ microbial decontamination of an ice-melting probe JF - Eurasian Chemico-Technological Journal. 12 (2010), H. 2 Y1 - 2010 SN - 1562-3920 SP - 145 EP - 150 ER - TY - JOUR A1 - Trzewik, Jürgen A1 - Temiz Artmann, Aysegül A1 - Linder, Peter A1 - Demirci, T. A1 - Digel, Ilya A1 - Artmann, Gerhard T1 - Evaluation of lateral mechanical tension in thin-film tissue constructs JF - Annals of Biomedical Engineering. 32 (2004), H. 9 Y1 - 2004 SN - 1573-9686 SP - 1243 EP - 1251 ER -