TY - JOUR A1 - Eilmann, Britta A1 - Rigling, Andreas ED - Abrams, Marc T1 - Tree-growth analyses to estimate tree species' drought tolerance JF - Tree Physiology N2 - Climate change is challenging forestry management and practices. Among other things, tree species with the ability to cope with more extreme climate conditions have to be identified. However, while environmental factors may severely limit tree growth or even cause tree death, assessing a tree species' potential for surviving future aggravated environmental conditions is rather demanding. The aim of this study was to find a tree-ring-based method suitable for identifying very drought-tolerant species, particularly potential substitute species for Scots pine (Pinus sylvestris L.) in Valais. In this inner-Alpine valley, Scots pine used to be the dominating species for dry forests, but today it suffers from high drought-induced mortality. We investigate the growth response of two native tree species, Scots pine and European larch (Larix decidua Mill.), and two non-native species, black pine (Pinus nigra Arnold) and Douglas fir (Pseudotsuga menziesii Mirb. var. menziesii), to drought. This involved analysing how the radial increment of these species responded to increasing water shortage (abandonment of irrigation) and to increasingly frequent drought years. Black pine and Douglas fir are able to cope with drought better than Scots pine and larch, as they show relatively high radial growth even after irrigation has been stopped and a plastic growth response to drought years. European larch does not seem to be able to cope with these dry conditions as it lacks the ability to recover from drought years. The analysis of trees' short-term response to extreme climate events seems to be the most promising and suitable method for detecting how tolerant a tree species is towards drought. However, combining all the methods used in this study provides a complete picture of how water shortage could limit species. Y1 - 2012 U6 - https://doi.org/10.1093/treephys/tps004 SN - 0829-318X (Print) SN - 1758-4469 (Online) VL - 32 IS - 2 SP - 178 EP - 187 PB - Oxford University Press CY - Oxford ER - TY - JOUR A1 - Rigling, Andreas A1 - Bigler, Christof A1 - Eilmann, Britta A1 - Feldmeyer-Christe, Elisabeth A1 - Gimmi, Urs A1 - Ginzler, Christian A1 - Graf, Ulrich A1 - Mayer, Phillip A1 - Vacchiano, Giorgio A1 - Weber, Pascal A1 - Wohlgemuth, Thomas A1 - zweifel, Roman A1 - Dobbertin, Matthias T1 - Driving factors of a vegetation shift from Scots pine to pubescent oak in dry Alpine forests JF - Global Change Biology N2 - An increasing number of studies have reported on forest declines and vegetation shifts triggered by drought. In the Swiss Rhone valley (Valais), one of the driest inner-Alpine regions, the species composition in low elevation forests is changing: The sub-boreal Scots pine (Pinus sylvestris L.) dominating the dry forests is showing high mortality rates. Concurrently the sub-Mediterranean pubescent oak (Quercus pubescens Willd.) has locally increased in abundance. However, it remains unclear whether this local change in species composition is part of a larger-scale vegetation shift. To study variability in mortality and regeneration in these dry forests we analysed data from the Swiss national forest inventory (NFI) on a regular grid between 1983 and 2003, and combined it with annual mortality data from a monitoring site. Pine mortality was found to be highest at low elevation (below 1000 m a.s.l.). Annual variation in pine mortality was correlated with a drought index computed for the summer months prior to observed tree death. A generalized linear mixed-effects model indicated for the NFI data increased pine mortality on dryer sites with high stand competition, particularly for small-diameter trees. Pine regeneration was low in comparison to its occurrence in the overstorey, whereas oak regeneration was comparably abundant. Although both species regenerated well at dry sites, pine regeneration was favoured at cooler sites at higher altitude and oak regeneration was more frequent at warmer sites, indicating a higher adaptation potential of oaks under future warming. Our results thus suggest that an extended shift in species composition is actually occurring in the pine forests in the Valais. The main driving factors are found to be climatic variability, particularly drought, and variability in stand structure and topography. Thus, pine forests at low elevations are developing into oak forests with unknown consequences for these ecosystems and their goods and services. Y1 - 2012 U6 - https://doi.org/10.1111/gcb.12038 SN - 1354-1013 (Print) SN - 1365-2486 (Online) VL - 19 IS - 1 SP - 229 EP - 240 PB - Wiley-Blackwell CY - Oxford ER - TY - CHAP A1 - Bohrn, Ulrich A1 - Mucha, Andreas A1 - Werner, Frederik A1 - Stütz, Evamaria A1 - Bäcker, Matthias A1 - Krumbe, Christoph A1 - Schienle, Meinrad A1 - Fleischer, Maximilian A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Detection of toxic chromium species in water using cellbased sensor systems Y1 - 2012 SN - 978-3-9813484-2-2 U6 - https://doi.org/10.5162/IMCS2012/P2.1.14 SP - 1364 EP - 1367 ER - TY - CHAP A1 - Bohrn, Ulrich A1 - Stütz, Evamaria A1 - Fleischer, Maximilian A1 - Schöning, Michael Josef A1 - Wagner, Patrick T1 - Living cell-based gas sensor system for the detection of acetone in air Y1 - 2012 SN - 978-3-9813484-2-2 U6 - https://doi.org/10.5162/IMCS2012/3.2.3 SP - 269 EP - 272 ER - TY - CHAP A1 - Takenaga, Shoko A1 - Werner, Frederik A1 - Sawada, Kazuaki A1 - Schöning, Michael Josef T1 - Comparison of label-free ACh image sensors based on CCD and LAPS Y1 - 2012 SN - 978-3-9813484-2-2 U6 - https://doi.org/10.5162/IMCS2012/4.2.6 SP - 356 EP - 359 ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Bäcker, Matthias T1 - Chip-basierte Sensoren für die Biotechnik Y1 - 2012 SN - 1611-0854 N1 - 4 Seiten VL - 13 IS - 2 PB - BIOCOM CY - Berlin ER - TY - JOUR A1 - Spelthahn, Heiko A1 - Schubert, Jürgen A1 - Schöning, Michael Josef T1 - Dünnschichtsensoren für die Schwermetallanalytik : Mikroelektroden auf Chalkogenidglasbasis JF - GIT Labor-Fachzeitschrift N2 - Die Detektion von Schadstoffen repräsentiert in der Umweltanalytik eine wichtige Aufgabenstellung. Gerade die Abwasser- bzw. Brauchwasseranalytik sowie die Prozesskontrolle haben einen hohen Stellenwert. Siliziumbasierte Dünnschichtsensoren bieten eine kostengünstige Möglichkeit, „online“-Messungen bzw. Vor-Ort-Messungen zeitnah durchzuführen. In dieser Arbeit wird ein potentiometrisches Sensorarray auf der Basis von Chalkogenidgläsern zur Detektion von Schwermetallen in wässrigen Medien vorgestellt. Y1 - 2012 IS - 4 SP - 285 EP - 287 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Fissabre, Anke A1 - Lohmann, Daniel T1 - Verlust einer „tragenden Wand“ : Das Haus der Jugend von Rudolf Schwarz in Aachen JF - Bauwelt Y1 - 2012 SN - 0005-6855 VL - 103 IS - 45 SP - 6 EP - 7 PB - Bauverlag BV CY - Gütersloh ER - TY - CHAP A1 - Fissabre, Anke A1 - Wilson, Ariane T1 - "Lehmbaupropaganda" - Gegensätzliche Ideologien über Nachhaltigkeit in der Literatur über den Lehmbau T1 - “Lehmbaupropaganda” - Contrasting ideologies of sustainability in earth building literature T2 - Lehm 2012 : Tagungsbeiträge der 6. Internationalen Fachtagung für Lehmbau = 6th International Conference on Building with Earth Y1 - 2012 SN - 9783000396496 SP - 200 EP - 213 PB - Eigenverl. Dachverband Lehm CY - Weimar ER - TY - CHAP A1 - Fissabre, Anke A1 - Schmidt, Klaus A1 - Sonnleitner, Andrea T1 - Das Lehnsmühlschloß in Ortrand – ein sächsisches Herrenhaus der Renaissance T2 - Wandel im Wohnbau zwischen Gotik und Barock : die sächsisch-böhmische Entwicklung im überregionalen Vergleich Y1 - 2012 SN - 978-3-89445-390-9 N1 - Jahrbuch für Hausforschung ; 53 SP - 487 EP - 501 PB - Jonas CY - Marburg ER - TY - CHAP A1 - Borchert, Jörg A1 - Lintzel, P. ED - Zenke, Ines T1 - Risiken des Handels T2 - Energiehandel in Europa Y1 - 2012 SN - 978-3-9813142-9-8 SP - 303 EP - 312 PB - Beck CY - München ET - 3. Aufl. ER - TY - CHAP A1 - Borchert, Jörg ED - Zenke, Ines T1 - Aufbau von Handelseinheiten T2 - Energiehandel in Europa Y1 - 2012 SN - 978-3-406-63237-2 SP - 467 EP - 479 PB - Beck CY - München ET - 3. Aufl. ER - TY - CHAP A1 - Borchert, Jörg A1 - Michels, A. ED - Zenke, ines T1 - Beschaffungsstrategien an der Schnittstelle Energiehandel / Vertrieb T2 - Energiehandel in Europa Y1 - 2012 SN - 978-3-406-63237-2 SP - 508 EP - 522 PB - Beck CY - München ET - 3. Aufl. ER - TY - CHAP A1 - Vismann, Ulrich T1 - Stahlbetonbau T2 - Wendehorst Beispiele aus der Baupraxis. - 4. Aufl. Y1 - 2012 SN - 978-3-8348-0999-5 ; 978-3-8348-8229-5 U6 - https://doi.org/10.1007/978-3-8348-8229-5_9 SP - 223 EP - 262 PB - Springer Vieweg CY - Wiesbaden ER - TY - CHAP A1 - Feiser, Johannes T1 - Geotechnik T2 - Wendehorst Beispiele aus der Baupraxis. - 4. Aufl. Y1 - 2012 SN - 978-3-8348-0999-5 ; 978-3-8348-8229-5 U6 - https://doi.org/10.1007/978-3-8348-8229-5_14 SP - 453 EP - 495 PB - Springer Vieweg CY - Wiesbaden ER - TY - CHAP A1 - Strohmeier, Andreas T1 - Siedlungswasserwirtschaft T2 - Wendehorst Beispiele aus der Baupraxis. - 4. Aufl. Y1 - 2012 SN - 978-3-8348-0999-5 ; 978-3-8348-8229-5 U6 - https://doi.org/10.1007/978-3-8348-8229-5_16 SP - 527 EP - 566 PB - Springer Vieweg CY - Wiesbaden ER - TY - CHAP A1 - Biener, Ernst T1 - Abfallwirtschaft T2 - Wendehorst Beispiele aus der Baupraxis. - 4. Aufl. Y1 - 2012 SN - 978-3-8348-0999-5 ; 978-3-8348-8229-5 U6 - https://doi.org/10.1007/978-3-8348-8229-5_17 SP - 567 EP - 603 PB - Springer Vieweg CY - Wiesbaden ER - TY - JOUR A1 - Henken, F. E. A1 - Oosterhuis, K. A1 - Öhlschläger, Peter A1 - Bosch, L. A1 - Hooijberg, E. A1 - Haanen, J. B. A. G. A1 - Steenbergen, R. D. M. T1 - Preclinical safety evaluation of DNA vaccines encoding modified HPV16 E6 and E7 JF - Vaccine N2 - Persistent infection with high-risk human papillomaviruses (hrHPV) can result in the formation of anogenital cancers. As hrHPV proteins E6 and E7 are required for cancer initiation and maintenance, they are ideal targets for immunotherapeutic interventions. Previously, we have described the development of DNA vaccines for the induction of HPV16 E6 and E7 specific T cell immunity. These vaccines consist of ‘gene-shuffled’ (SH) versions of HPV16 E6 and E7 that were fused to Tetanus Toxin Fragment C domain 1 (TTFC) and were named TTFC-E6SH and TTFC-E7SH. Gene-shuffling was performed to avoid the risk of inducing malignant transformation at the vaccination site. Here, we describe the preclinical safety evaluation of these candidate vaccines by analysis of their transforming capacity in vitro using established murine fibroblasts (NIH 3T3 cells) and primary human foreskin keratinocytes (HFKs). We demonstrate that neither ectopic expression of TTFC-E6SH and TTFC-E7SH alone or in combination enabled NIH 3T3 cells to form colonies in soft agar. In contrast, expression of HPV16 E6WT and E7WT alone or in combination resulted in effective transformation. Similarly, retroviral transduction of HFKs from three independent donors with both TTFC-E6SH and TTFC-E7SH alone or in combination did not show any signs of immortalization. In contrast, the combined expression of E6WT and E7WT induced immortalization in HFKs from all donors. Based on these results we consider it justified to proceed to clinical evaluation of DNA vaccines encoding TTFC-E6SH and TTFC-E7SH in patients with HPV16 associated (pre)malignancies. Y1 - 2012 U6 - https://doi.org/10.1016/j.vaccine.2012.04.013 SN - 0264-410X VL - 30 IS - 28 SP - 4259 EP - 4266 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Immel, Timo A1 - Grützke, Martin A1 - Späte, Anne-Katrin A1 - Groth, Ulrich A1 - Öhlschläger, Peter A1 - Huhn, Thomas T1 - Synthesis and X-ray structure analysis of a heptacoordinate titanium(IV)-bis-chelate with enhanced in vivo antitumor efficacy JF - Chemical Communications N2 - Chelate stabilization of a titanium(IV)–salan alkoxide by ligand exchange with 2,6-pyridinedicarboxylic acid (dipic) resulted in heptacoordinate complex 3 which is not redox-active, stable on silica gel and has increased aqueous stability. 3 is highly toxic in HeLa S3 and Hep G2 and has enhanced antitumor efficacy in a mouse cervical-cancer model. Y1 - 2012 U6 - https://doi.org/10.1039/C2CC31624B SN - 1364-548X VL - 48 IS - 46 SP - 5790 EP - 5792 PB - Royal Society of Chemistry CY - Cambridge ER - TY - BOOK A1 - Vismann, Ulrich T1 - Wendehorst Beispiele aus der Baupraxis / Ulrich Vismann (Hrsg.). - 4., durchges. und aktualis. Aufl. Y1 - 2012 SN - 978-3-8348-0999-5 ; 978-3-8348-8229-5 U6 - https://doi.org/10.1007/978-3-8348-8229-5 PB - Springer Vieweg CY - Wiesbaden ER - TY - CHAP A1 - Hoffschmidt, Bernhard A1 - Alexopoulos, Spiros T1 - Perspektiven für Solarthermische Kraftwerke im Sonnengürtel T2 - Energiewende : Aspekte, Optionen, Herausforderungen : Vorträge auf der DPG-Frühjahrstagung Arbeitskreis Energie in der Deutschen Physikalischen Gesellschaft Berlin, 26. bis 28. März 2012 / hrsg. von Hardo Bruhns Y1 - 2012 SP - 81 EP - 93 PB - DPG CY - Baf Honnef ER - TY - RPRT A1 - Butenweg, Christoph A1 - Dargel, H.-J. A1 - Höchst, T. A1 - Holtschoppen, B. A1 - Schwarz, R. A1 - Sippel, M. T1 - Der Lastfall Erdbeben im Anlagenbau : Leitfaden : Entwurf, Bemessung und Konstruktion von Tragwerken und Komponenten in der chemischen Industrie in Anlehnung an die DIN EN 1998-1 Y1 - 2012 PB - Verband der Chemischen Industrie e.V. CY - Frankfurt ER - TY - CHAP A1 - Bung, Daniel Bernhard T1 - Sensitivity of phase detection techniques in aerated chute flows to hydraulic design parameters T2 - 2nd European IAHR congress : 27. - 29. June 2012, München Y1 - 2012 SN - 978-3-943683-03-5 SP - Artikelkennnummer: B15 PB - Lehrstuhl u. Versuchsanst. für Wasserbau u. Wasserwirtschaft d. TU München CY - München ER - TY - CHAP A1 - Fricke, Barbara A1 - Ziolko, C. A1 - Anthrakidis, Anette A1 - Alexopoulos, Spiros A1 - Hoffschmidt, Bernhard A1 - Giese, F. A1 - Dillig, M. T1 - InnoSol - environmental aspects of the open volumetric receiver technology T2 - 30th ISES Biennial Solar World Congress 2011 : : Kassel, Germany, 28 August - 2 September 2011. Vol. 5 Y1 - 2012 SP - 3895 EP - 3900 PB - Curran CY - Red Hook, NY ER - TY - BOOK A1 - Bialonski, Stephan T1 - Inferring complex networks from time series of dynamical systems: Pitfalls, misinterpretations, and possible solutions Y1 - 2012 N1 - Bonn, Rheinische Friedrich-Wilhelms-Universität Bonn, Diss., 2012 PB - Universitäts- und Landesbibliothek Bonn CY - Bonn ER - TY - JOUR A1 - Backes, Klaus A1 - Adam, Mario A1 - Gottschald, Beng Jonas A1 - Faber, Christian A1 - Henneböhl, Friedrich-Gregor A1 - Lanz, Marco A1 - Anthrakidis, Anette T1 - KWKK-Anlagen im Feldtest. Optimaler Betrieb durch Verbesserungen bei Regelung und Hydraulik JF - Die Kälte- und Klimatechnik Y1 - 2012 SN - 0343-2246 IS - 2 PB - Gentner CY - Stuttgart ER - TY - CHAP A1 - Sauerborn, Markus A1 - Klimek, J. A1 - Hoffschmidt, Bernhard A1 - Essen, H. A1 - Sieger, S. A1 - Biegel, G. A1 - Göttsche, Joachim A1 - Hilger, Patrick T1 - Eurosun 2012 : radar technology for heliostat posititon control T2 - Eurosun 2012 : Solar energy for a brighter future : conference proceedings : Rijeka, 18.-22.09.2012 Y1 - 2012 SP - ID 80 CY - Rijeka ER - TY - CHAP A1 - Duong, Minh Tuan A1 - Nguyen, Nhu Hunyh A1 - Staat, Manfred T1 - Finite Element Implementation of a 3D Fung-type Model T2 - ESMC-2012 - 8th European Solid Mechanics Conference, Graz, Austria, July 9-13, 2012 Y1 - 2012 SN - 978-3-85125-223-1 PB - Verlag d. Technischen Universität Graz CY - Graz ER - TY - GEN A1 - Lindel, Tomasz Dawid A1 - Greiser, Andreas A1 - Waxman, Patrick A1 - Dietterle, Martin A1 - Seifert, Frank A1 - Fontius, Ulrich A1 - Renz, Wolfgang A1 - Dieringer, Matthias A. A1 - Frauenrath, Tobias A1 - Schulz-Menger, Jeanette A1 - Niendorf, Thoralf A1 - Ittermann, Bernd T1 - Cardiac CINE MRI at 7 T using a transmit array T2 - 2012 ISMRM Annual Meeting Proceedings N2 - With its need for high SNR and short acquisition times, Cardiac MRI (CMR) is an intriguing target application for ultrahigh field MRI. Due to the sheer size of the upper torso, however, the known RF issues of 7T MRI are also most prominent in CMR. Recent years brought substantial progress but the full potential of the ultrahigh field for CMR is yet to be exploited. Parallel transmission (pTx) is a promising approach in this context and several groups have already reported B1 shimming for 7T CMR. In such a static pTx application amplitudes and phases of all Tx channels are adjusted individually but otherwise imaging techniques established in current clinical practice 1.5 T and 3 T are applied. More advanced forms of pTx as spatially selective excitation (SSE) using Transmit SENSE promise additional benefits like faster imaging with reduced fields of view or improved SAR control. SSE requires the full dynamic capabilities of pTx, however, and for the majority of today's implemented pTx hardware the internal synchronization of the Tx array does not easily permit external triggering as needed for CMR. Here we report a software solution to this problem and demonstrate the feasibility of CINE CMR at 7 T using a Tx array. Y1 - 2012 SN - 1545-4428 N1 - ISMRM 20th Annual Meeting & Exhibition, 5-11 May 2012, Melbourne, Australia ER - TY - CHAP A1 - Fabo, Sabine T1 - Einführung T2 - Vielen Dank für Ihren Einkauf : Konsumkultur aus Sicht von Design, Kunst und Medien Y1 - 2012 SN - 978-3-8376-2170-9 SP - 9 EP - 13 PB - Transcript Verlag CY - Bielefeld ER - TY - GEN A1 - Tippkötter, Nils A1 - Pasteur, A. A1 - Ulber, Roland T1 - Magnetisch abtrennbare Gold-Nanopartikel zur katalytischen Zuckeroxidation T2 - Chemie Ingenieur Technik N2 - Glucose ist ein primäres Zwischenprodukt der Verarbeitung nachwachsender Rohstoffe, wie z. B. Cellulose. Die wertsteigernde Weiterverarbeitung des Monosaccharids erfolgt häufig in Form vonFermentationsprozessen, jedoch kann der Rohstoff auch für zahlreiche chemische Verarbeitungsstufen genutzt werden. Ein großtechnisch relevanter Prozess ist die Herstellung von Gluconsäure (GS), die u. a. als Nahrungsmittelzusatz (E 574) eingesetzt wird. Die Darstellung der Säure erfolgt durch Oxidation von Glucose mit magnetisierbarem Gold-Nano-Katalysator. Die Rückgewinnung des Katalysators aus der Reaktionslösung wurde unter Einwirkung eines Magnetfeldgradienten verwirklicht. Die Synthese der magnetischen Goldkatalysatoren (sowohl Trägerpartikel als auch Gold-Nanopartikel) wurde durch nass-chemische Fällungsreaktionen durchgeführt. Die Charakteristiken der neuen Materialen konnte durch Messungen des PCD-Potenzials, Laserbeugung und REM/EDX untersucht werden. So wurden u. a. Partikeldurchmesser von 25 lm und ein Goldgehaltvon 1,03 % ermittelt. Weiterhin wurden für die Goldkatalysatoren optimale Reaktionsbedingungen für die Glucoseoxidation im geregelten Rührkesselreaktor etabliert. Hierdurch konnten eine Produktselektivität von 100 % und eine Wiederverwendbarkeit der Partikel über mindestens zehn Zyklen erreicht werden. Y1 - 2012 U6 - https://doi.org/10.1002/cite.201250393 SN - 0009-286X SN - 1522-2640 (eISSN) N1 - ProcessNet-Jahrestagung 2012 und 30. DECHEMA-Jahrestagung der Biotechnologen, 10. – 13. September 2012, Karlsruhe VL - 84 IS - 8 SP - 1328 PB - Wiley-VCH CY - Weinheim ER - TY - CHAP A1 - Sieker, T. A1 - Duwe, A. A1 - Poth, S. A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - Herstellung von Itaconsäure aus Buchenholzhydrolysaten T2 - Kurzfassungsband / GVC-DECHEMA Vortrags- und Diskussionstagung Biopharmazeutische Produktion : 14. - 16. Mai 2012. Konzerthaus Freibung Y1 - 2012 SP - 57 PB - DECHEMA CY - Frankfurt, M. ER - TY - JOUR A1 - Ferrein, Alexander A1 - Steinbauer, Gerald A1 - Vassos, Stavros T1 - Action-Based Imperative Programming with YAGI N2 - Many tasks for autonomous agents or robots are best described by a specification of the environment and a specification of the available actions the agent or robot can perform. Combining such a specification with the possibility to imperatively program a robot or agent is what we call the actionbased imperative programming. One of the most successful such approaches is Golog. In this paper, we draft a proposal for a new robot programming language YAGI, which is based on the action-based imperative programming paradigm. Our goal is to design a small, portable stand-alone YAGI interpreter. We combine the benefits of a principled domain specification with a clean, small and simple programming language, which does not exploit any side-effects from the implementation language. We discuss general requirements of action-based programming languages and outline YAGI, our action-based language approach which particularly aims at embeddability. Y1 - 2012 N1 - Cognitive Robotics AAAI Technical Report WS-12-06 SP - 24 EP - 31 ER - TY - GEN A1 - Stadtmüller, R. A1 - Wollny, S. A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - Amplifikation und Einsatz von ssDNA-Aptameren T2 - Chemie Ingenieur Technik N2 - Die wachsende Produktpalette von z. B. Pharmazeutika geht mit einer steigenden Nachfrage für hochsensitive/schonende Aufreinigungstechniken einher. Bisherige Verfahren führen oft zu geringer Reinheit und verminderter Bioaktivität, zeigen eine Limitation der Analytengröße oder bedingen dessen Modifikation. Durch die Kombination von mikroskaligen Magnetpartikeln und spezifisch wechselwirkenden Einzelstrang-DNA-Oligonukleotiden, den sog. ssDNA-Aptameren, sind eine höhere Selektivität/Reinheit und eine Automatisierung möglich. In diesem Kontext werden zum einen ssDNA-Amplifikationstechniken und zum anderen der praktische Einsatz von Aptameren in einer Magnetseparation vorgestellt. Die ssDNA-Synthese basiert auf einem In-vivo-dsDNA-Produktionsschritt mittels eines rekombinanten Escherichia coli. Die als High-copy-Plasmid organisierte Sequenz wird in vitro durch Kombination verschiedener enzymatischer Reaktionen in die funktionelle ssDNA überführt. Diese Technik bedingt nur minimale Instrumentierung bzw. Prozessregelung. Die zweite Synthesetechnik wird in Form eines In-vitro-Amplifikationsverfahrens realisiert und beruht auf dem Prinzip einer PCR (Potenzial zu einer Automatisierung bzw. Miniaturisierung). Die gewonnenen Aptamere werden im Anschluss in einem auf Magnetpartikeln basierten Trennverfahren zur Isolationvon 6xHis-tag-Proteinen bezüglich ihrer Eigenschaften untersucht. Y1 - 2012 U6 - https://doi.org/10.1002/cite.201250112 SN - 0009-286X SN - 1522-2640 (eISSN) N1 - ProcessNet-Jahrestagung 2012 und 30. DECHEMA-Jahrestagung der Biotechnologen, 10. – 13. September 2012, Karlsruhe VL - 84 IS - 8 SP - 1294 PB - Wiley-VCH CY - Weinheim ER - TY - CHAP A1 - Achenbach, Timm A1 - Geimer, Konstantin A1 - Lynen, Arthur A1 - Göttsche, Joachim A1 - Hoffschmidt, Bernhard T1 - Simulation of thermo-mechanical processes in open volumetric absorber modules T2 - SolarPaces 2012 : concentrating solar power and chemical energy systems : Sept. 11 - 14 2012, Marrakech, Marokko Y1 - 2012 SP - 1 EP - 8 ER - TY - CHAP A1 - Hoffschmidt, Bernhard A1 - Alexopoulos, Spiros A1 - Göttsche, Joachim A1 - Sauerborn, Markus T1 - High concentration solar collectors T2 - Comprehensive renewable energy / ed. Ali Sayigh. Vol. 3: Solar thermal systems: components and applications N2 - Solar thermal concentrated power is an emerging technology that provides clean electricity for the growing energy market. To the solar thermal concentrated power plant systems belong the parabolic trough, the Fresnel collector, the solar dish, and the central receiver system. For high-concentration solar collector systems, optical and thermal analysis is essential. There exist a number of measurement techniques and systems for the optical and thermal characterization of the efficiency of solar thermal concentrated systems. For each system, structure, components, and specific characteristics types are described. The chapter presents additionally an outline for the calculation of system performance and operation and maintenance topics. One main focus is set to the models of components and their construction details as well as different types on the market. In the later part of this chapter, different criteria for the choice of technology are analyzed in detail. KW - Central receiver system KW - Concentrated solar collector KW - Fresnel collector KW - Optical and thermal analysis KW - Solar concentration Y1 - 2012 SN - 978-0-08-087873-7 U6 - https://doi.org/10.1016/B978-0-08-087872-0.00306-1 VL - 3 SP - 165 EP - 209 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Kurulgan Demirci, Eylem A1 - Demirci, Taylan A1 - Linder, Peter A1 - Trzewik, Jürgen A1 - Gierkowski, Jessica Ricarda A1 - Gossmann, Matthias A1 - Kayser, Peter A1 - Porst, Dariusz A1 - Digel, Ilya A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül T1 - rhAPC reduces the endothelial cell permeability via a decrease of contractile tensions induced by endothelial cells JF - Journal of Bioscience and Bioengineering N2 - All cells generate contractile tension. This strain is crucial for mechanically controlling the cell shape, function and survival. In this study, the CellDrum technology quantifying cell's (the cellular) mechanical tension on a pico-scale was used to investigate the effect of lipopolysaccharide (LPS) on human aortic endothelial cell (HAoEC) tension. The LPS effect during gram-negative sepsis on endothelial cells is cell contraction causing endothelium permeability increase. The aim was to finding out whether recombinant activated protein C (rhAPC) would reverse the endothelial cell response in an in-vitro sepsis model. In this study, the established in-vitro sepsis model was confirmed by interleukin 6 (IL-6) levels at the proteomic and genomic levels by ELISA, real time-PCR and reactive oxygen species (ROS) activation by florescence staining. The thrombin cellular contraction effect on endothelial cells was used as a positive control when the CellDrum technology was applied. Additionally, the Ras homolog gene family, member A (RhoA) mRNA expression level was checked by real time-PCR to support contractile tension results. According to contractile tension results, the mechanical predominance of actin stress fibers was a reason of the increased endothelial contractile tension leading to enhanced endothelium contractility and thus permeability enhancement. The originality of this data supports firstly the basic measurement principles of the CellDrum technology and secondly that rhAPC has a beneficial effect on sepsis influenced cellular tension. The technology presented here is promising for future high-throughput cellular tension analysis that will help identify pathological contractile tension responses of cells and prove further cell in-vitro models. KW - Cell permeability KW - Cellular force KW - Endothelial cells KW - Recombinant activated protein C KW - Lipopolysaccharide KW - Contractile tension KW - CellDrum Y1 - 2012 U6 - https://doi.org/10.1016/j.jbiosc.2012.03.019 SN - 1347-4421 VL - 113 IS - 2 SP - 212 EP - 219 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Rosemann, Michael A1 - Eggert, Mathias A1 - Voigt, Matthias A1 - Beverungen, Daniel T1 - Leveraging Social Network Data for Analytical CRM Strategies - The Introduction of Social BI. T2 - ECIS 2012 Proceedings Y1 - 2012 N1 - European Conference on Information Systems (ECIS), 2012 ER - TY - GEN A1 - Thiel, A. A1 - Tippkötter, Nils A1 - Muffler, K. A1 - Ruf, F. A1 - Sohling, U. A1 - Ulber, Roland T1 - Optimierung der Wertschöpfungskette bei der Aufarbeitung von Rapsschrot mit Zeolithen T2 - Chemie Ingenieur Technik N2 - Im vom BMELV/FNR geförderten SynRg-Projekt wurde unter anderem Rapsschrot untersucht, um Polyphenole zu isolieren und aufzureinigen. Diese sollen anschließend als Basisbausteine für Polymere dienen und ihnen neuartige Eigenschaften verleihen. Derzeit wird an der Polyphenolextraktion gearbeitet, da bei organischen oder wässrigen Extraktionsprozessen überwiegend Sinapin, ein Cholinester der Sinapinsäure, vorliegt und dieses nicht für die Polymerbildung eingesetzt werden kann. Für die im Fokus stehende Sinapinsäure wird deshalb eine simultane Extraktion und enzymatische oder chemische Hydrolyse von Sinapin zu Sinapinsäure durchgeführt. Durch die Hydrolyse konnte die Sinapinsäureausbeute bereits um den Faktor 6,2 auf 15,8 mg g⁻¹ gegenüber einer reinwässrigen Extraktion gesteigert werden. Für die Aufreinigung des an Sinapinsäure reichen Extrakts erfolgt anschließend ein adsorptiver Aufarbeitungsschritt, bei dem Zeolithe zum Einsatz kommen. Mit diesem Material ist es möglich, die Sinapinsäure quantitativ zu adsorbieren und später mit 70 %igem Ethanol bei 60 °C zu desorbieren. Bei den Adsorbern handelt es sich um b-Zeolithe von der Süd-Chemie AG. Y1 - 2012 U6 - https://doi.org/10.1002/cite.201250028 SN - 0009-286X SN - 1522-2640 (eISSN) N1 - ProcessNet-Jahrestagung 2012 und 30. DECHEMA-Jahrestagung der Biotechnologen, 10. – 13. September 2012, Karlsruhe N1 - Dieses Vorhaben wird aus Mitteln des Bundesministeriums für Ernährung, Landwirtschaft und Verbraucherschutz (BMELV) durch seinen Projektträger der Fachagentur Nachwachsende Rohstoffe e.V. (FNR) unter der Projektnummer22023008 gefördert. VL - 84 IS - 8 SP - 1191 EP - 1192 PB - Wiley-VCH CY - Weinheim ER - TY - CHAP A1 - Dachwald, Bernd A1 - Feldmann, Marco A1 - Espe, Clemens A1 - Plescher, Engelbert A1 - Konstantinidis, K. A1 - Forstner, R. T1 - Enceladus explorer - A maneuverable subsurface probe for autonomous navigation through deep ice T2 - 63rd International Astronautical Congress 2012, IAC 2012; Naples; Italy; 1 October 2012 through 5 October 2012. (Proceedings of the International Astronautical Congress, IAC ; 3) Y1 - 2012 SN - 978-1-62276-979-7 SP - 1756 EP - 1766 PB - Curran CY - Red Hook, NY ER - TY - GEN A1 - Sieker, T. A1 - Duwe, A. A1 - Poth, S. A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - Itaconsäureherstellung aus Buchenholz-Hydrolysaten T2 - Chemie Ingenieur Technik N2 - Aus hölzernen Cellulosen und Hemicellulosen können durch enzymatische Hydrolyse fermentierbare Zucker für die Herstellung von Chemikalien und Treibstoffen gewonnen werden. Die bisherige Forschung fokussiert sich oft auf die Nutzung dieser Zucker zur Gewinnung von Ethanol. Daneben muss aber auch die stoffliche Nutzung zur Gewinnung von Grundchemikalien berücksichtigt werden. Eine solche Grundchemikalie ist Itakonsäure. Obwohl die biotechnologische Itaconsäureproduktion bereits eingehend untersucht und etabliert ist, gestaltet sie sich im Rahmen von Bioraffinerien der zweiten Generation als schwierig, da der überwiegend verwendete Produktionsorganismus gegen eine weite Bandbreite von Inhibitoren sensibel ist. Die Herstellung von Itaconsäure aus Buchenholzhydrolysaten wird im Rahmen der deutschen Lignocellulose-Bioraffinerie entwickelt. Die unbehandelten Hydrolysate ermöglichen weder das Wachstum von Aspergillus terreus noch die Bildung von Itaconsäure. Daher werden Möglichkeiten zur Konditionierung des Hydrolysates mit dem Ziel einer Itaconsäureproduktion mit hohen Ausbeuten und Konzentrationen vorgestellt. Y1 - 2012 U6 - https://doi.org/10.1002/cite.201250414 SN - 0009-286X SN - 1522-2640 (eISSN) N1 - ProcessNet-Jahrestagung 2012 und 30. DECHEMA-Jahrestagung der Biotechnologen, 10. – 13. September 2012, Karlsruhe N1 - Die präsentierte Arbeit wird vomBMELV über die FNR gefördert (Förderkennzeichen 22019409). VL - 84 IS - 8 SP - 1300 PB - Wiley-VCH CY - Weinheim ER - TY - BOOK A1 - Wählisch, Georg T1 - Technisches Zeichnen : Lösungsvorschläge für das Selbststudium. (Berichte aus dem Maschinenbau) Y1 - 2012 SN - 978-3-8440-1159-3 PB - Shaker CY - Aachen ER - TY - JOUR A1 - Oertel, Mario A1 - Bung, Daniel Bernhard T1 - Initial stage of two-dimensional dam-break waves: laboratory versus VOF JF - Journal of hydraulic research N2 - Since several decades, dam-break waves have been of main research interest. Mathematical approaches have been developed by analytical, physical and numerical models within the past 120 years. During the past 10 years, the number of research investigations has increased due to improved measurement techniques as well as significantly increased computer memories and performances. In this context, the present research deals with the initial stage of two-dimensional dam-break waves by comparing physical and numerical model results as well as analytical approaches. High-speed images and resulting particle image velocimetry calculations are thereby compared with the numerical volume-of-fluid (VOF) method, included in the commercial code FLOW-3D. Wave profiles and drag forces on placed obstacles are analysed in detail. Generally, a good agreement between the laboratory and VOF results is found. KW - VOF KW - PIV KW - physical model KW - numerical model KW - drag force KW - dam-break Y1 - 2012 U6 - https://doi.org/10.1080/00221686.2011.639981 SN - 1814-2079 (E-Journal); 0022-1686 (Print) VL - 50 IS - 1 SP - 89 EP - 97 PB - Taylor & Francis CY - London ER - TY - GEN A1 - Sieker, T. A1 - Tippkötter, Nils A1 - Muffler, K. A1 - Ulber, Roland T1 - Herstellung von Ethanol, Phenolsäuren, organischen Säuren und Biogas durch vollständige Nutzung von Grassilage in einer Bioraffinerie T2 - Chemie Ingenieur Technik N2 - Gräser sind in der Lage, einen großen Teil der für eine biobasierte Wirtschaft benötigten Biomasse zur Verfügung zustellen. Um eine ganzjährige Nutzung des Grases zu gewährleisten, muss eine stabile Lagerung des Grases erreicht werden, was z. B. durch Silieren möglich ist. Die konservierende Wirkung der Silierung beruht auf der Bildung organischer Säuren. Um diese zu gewinnen, wird die Silage gepresst, die organischen Säuren über Flüssig/Flüssig-Extraktion aus dem Presssaft abgetrenntund mittels chromatographischer Methoden weiter aufgereinigt. Im präsentierten Konzept werden die im Presskuchen enthaltenen Lignocellulosen hydrolysiert und die erhaltenen Monosaccharide zu Ethanol fermentiert. Die Phenolsäuren, die in Gräsern die Rolle des Lignins übernehmen, können simultan mit der Hydrolyse der Polysaccharide enzymatisch abgetrennt und als Nebenprodukt gewonnen werden. Die nach der Abtrennung des Ethanols verbleibenden Fermentationsreststoffe werden für die Herstellung von Biogas verwendet. Y1 - 2012 U6 - https://doi.org/10.1002/cite.201250415 SN - 0009-286X SN - 1522-2640 (eISSN) N1 - ProcessNet-Jahrestagung 2012 und 30. DECHEMA-Jahrestagung der Biotechnologen, 10. – 13. September 2012, Karlsruhe N1 - Die präsentierte Arbeit wird vom BMELV über die FNR gefördert (Förder-kennzeichen 22025407). VL - 84 IS - 8 SP - 1297 PB - Wiley-VCH CY - Weinheim ER - TY - CHAP A1 - Becker, Jörg A1 - Eggert, Mathias A1 - Fleischer, Stefan A1 - Heddier, Marcel A1 - Knackstedt, Ralf T1 - Data Warehouse Design and Legal Visualization – The Applicability of H2 for Reporting T2 - Proceedings of the 23rd Australasian Conference on Information Systems 2012 Y1 - 2012 N1 - Australasian Conference on Information Systems (23rd : 2012 : Geelong, Victoria) ER - TY - JOUR A1 - Nguyen, Nhu Huynh A1 - Duong, Minh Tuan A1 - Tran, Thanh Ngoc A1 - Pham, Phu Tinh A1 - Grottke, O. A1 - Tolba, R. A1 - Staat, Manfred T1 - Influence of a freeze–thaw cycle on the stress–stretch curves of tissues of porcine abdominal organs JF - Journal of Biomechanics N2 - The paper investigates both fresh porcine spleen and liver and the possible decomposition of these organs under a freeze–thaw cycle. The effect of tissue preservation condition is an important factor which should be taken into account for protracted biomechanical tests. In this work, tension tests were conducted for a large number of tissue specimens from twenty pigs divided into two groups of 10. Concretely, the first group was tested in fresh state; the other one was tested after a freeze-thaw cycle which simulates the conservation conditions before biomechanical experiments. A modified Fung model for isotropic behavior was adopted for the curve fitting of each kind of tissues. Experimental results show strong effects of the realistic freeze–thaw cycle on the capsule of elastin-rich spleen but negligible effects on the liver which virtually contains no elastin. This different behavior could be explained by the autolysis of elastin by elastolytic enzymes during the warmer period after thawing. Realistic biomechanical properties of elastin-rich organs can only be expected if really fresh tissue is tested. The observations are supported by tests of intestines. KW - Autolysis KW - Decomposition KW - Freeze–thaw process KW - Spleen KW - Liver Y1 - 2012 U6 - https://doi.org/10.1016/j.jbiomech.2012.07.008 SN - 1873-2380 VL - 45 IS - 14 SP - 2382 EP - 2386 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Staat, Manfred A1 - Trenz, Eva A1 - Lohmann, Philipp A1 - Frotscher, Ralf A1 - Klinge, Uwe A1 - Tabaza, Ruth A1 - Kirschner-Hermanns, Ruth T1 - New measurements to compare soft tissue anchoring systems in pelvic floor surgery JF - Journal of Biomedical Materials Research Part B: Applied Biomaterials N2 - Suburethral slings as well as different meshes are widely used treating stress urinary incontinence and prolaps in women. With the development of MiniSlings and special meshes using less alloplastic material anchorage systems become more important to keep devices in place and to put some tension especially on the MiniSlings. To date, there are many different systems of MiniSlings of different companies on the market which differ in the structure of the used meshes and anchors. A new objective measurement method to compare different properties of MiniSling systems (mesh and anchor) is presented in this article. Ballistic gelatine acts as soft tissue surrogate. Significant differences in parameters like pull-out strength of anchors or shrinkage of meshes under loading conditions have been determined. The form and size of the anchors as well as the structural stability of the meshes are decisive for a proper integration. The tested anchorings sytems showed markedly different mechanical function at their respective load bearing capacity. As the stable fixation of the device in tissue is a prerequisite for a permanet reinforcement, the proposed test system permits further optimisation of anchor and mesh devices to improve the success of the surgical treatment Y1 - 2012 U6 - https://doi.org/10.1002/jbm.b.32654 SN - 1552-4981 VL - 100B IS - 4 SP - 924 EP - 933 PB - Wiley CY - Hoboken, NJ ER - TY - JOUR A1 - Novacek, V. A1 - Tran, Thanh Ngoc A1 - Klinge, U. A1 - Tolba, R. H. A1 - Staat, Manfred A1 - Bronson, D. G. A1 - Miesse, A. M. A1 - Whiffen, J. A1 - Turquier, F. T1 - Finite element modelling of stapled colorectal end-to-end anastomosis : Advantages of variable height stapler design JF - Journal of Biomechanics N2 - The impact of surgical staplers on tissues has been studied mostly in an empirical manner. In this paper, finite element method was used to clarify the mechanics of tissue stapling and associated phenomena. Various stapling modalities and several designs of circular staplers were investigated to evaluate the impact of the device on tissues and mechanical performance of the end-to-end colorectal anastomosis. Numerical simulations demonstrated that a single row of staples is not adequate to resist leakage due to non-linear buckling and opening of the tissue layers between two adjacent staples. Compared to the single staple row configuration, significant increase in stress experienced by the tissue at the inner staple rows was observed in two and three rows designs. On the other hand, adding second and/or third staple row had no effect on strain in the tissue inside the staples. Variable height design with higher staples in outer rows significantly reduced the stresses and strains in outer rows when compared to the same configuration with flat cartridge. KW - Variable height stapler design KW - Anastomotic leakage KW - Finite element modelling KW - End-to-end colorectal anastomosis KW - Surgical staplers Y1 - 2012 U6 - https://doi.org/10.1016/j.jbiomech.2012.07.021 SN - 1873-2380 VL - 45 IS - 115 SP - 2693 EP - 2697 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Verbanella, Stresa A1 - Fabo, Sabine T1 - Mit Pinsel und Farbe hacken : Interview mit Sabine Fabo T1 - Hacking with paint and brush : interview with Sabine Fabo JF - Remotewords.net N2 - Remotewords makes its mark on the picturesque "Bar Verbanella”, located in Stresa near Isola Bella on Lago Maggiore. The words CUM GRANO SALIS (with a grain of salt) by Sabine Fabo, art theorist on media contexts, were applied to its roof in collaboration with the German-Italian art organization "Lo spirito del Lago". KW - Guerrillawerbung KW - Parasitäre Strategie Y1 - 2012 ER - TY - JOUR A1 - Bassam, Rasha A1 - Hescheler, Jürgen A1 - Temiz Artmann, Aysegül A1 - Artmann, Gerhard A1 - Digel, Ilya T1 - Effects of spermine NONOate and ATP on the thermal stability of hemoglobin JF - BMC Biophysics N2 - Background Minor changes in protein structure induced by small organic and inorganic molecules can result in significant metabolic effects. The effects can be even more profound if the molecular players are chemically active and present in the cell in considerable amounts. The aim of our study was to investigate effects of a nitric oxide donor (spermine NONOate), ATP and sodium/potassium environment on the dynamics of thermal unfolding of human hemoglobin (Hb). The effect of these molecules was examined by means of circular dichroism spectrometry (CD) in the temperature range between 25°C and 70°C. The alpha-helical content of buffered hemoglobin samples (0.1 mg/ml) was estimated via ellipticity change measurements at a heating rate of 1°C/min. Results Major results were: 1) spermine NONOate persistently decreased the hemoglobin unfolding temperature T u irrespectively of the Na + /K + environment, 2) ATP instead increased the unfolding temperature by 3°C in both sodium-based and potassium-based buffers and 3) mutual effects of ATP and NO were strongly influenced by particular buffer ionic compositions. Moreover, the presence of potassium facilitated a partial unfolding of alpha-helical structures even at room temperature. Conclusion The obtained data might shed more light on molecular mechanisms and biophysics involved in the regulation of protein activity by small solutes in the cell. KW - Nitric Oxide Donor KW - NONOate KW - Circular Dichroism KW - Nitric Oxide Y1 - 2012 U6 - https://doi.org/10.1186/2046-1682-5-16 SN - 2046-1682 VL - 5 PB - BioMed Central CY - London ER -