TY  - GEN
A1  - Engel, Mareike
A1  - Bayer, Hendrik
A1  - Ulber, Roland
A1  - Tippkötter, Nils
T1  - Kommt es in Elektrofermentationen mit Clostridium acetobutylicum zu einer Eisenlimitierung?
T2  - Chemie Ingenieur Technik
N2  - Das strikt anaerobe Bakterium Clostridium acetobutylicum bildet die Lösemittel Aceton, Butanol und Ethanol (ABE-Fermentation). Im Fall einer Eisenlimitierung kommt es zusätzlich zu einer Riboflavinsekretion (RF), was durch die gelbe Färbung des Kulturüberstands erkennbar ist. In dieser Arbeit wurde beobachtet, dass während Elektrofermentationen mit C. acetobutylicum bei –600 mV eine gelbe Färbung auftritt. Es wurde deshalb untersucht, ob eine Eisenlimitierung im bio-elektrochemischen System (BES) vorliegt. Hierzu wurden die Flavinspezien bei Kultivierungen in Medien mit einer Eisenlimitierung bzw. mit ausreichend Eisen in Serumflaschen sowie im BES mit und ohne angelegtem Potenzial verglichen. In den Serumflaschenversuchen wurden RF und Flavinadenindinukleotid (FAD)-Konzentrationen von ‡ 20 mg L⁻¹ sowie Flavinmononukleotid (FMN)-Konzentrationen von ca. 5 mg L⁻¹ detektiert. Bei ausreichender Eisenverfügbarkeit hingegen wurden in den Serumflaschen fast keine Flavine sekretiert. Im BES bei –600 mV hingegen wurde auch in diesen Kulturüberständen FMN und FAD (1–5 mg L⁻¹ ),jedoch kein RF gemessen. Diese Ergebnisse zeigen, dass die Flavinbildung im BES mit angelegtem Potenzial nicht mit der Flavinbildung unter Eisenlimitierung in Serumflaschen korreliert. Andere Faktoren für eine mögliche Flavinbildung werden aktuell näher betrachtet.
Y1  - 2018
U6  - https://doi.org/10.1002/cite.201855047
SN  - 0009-286X
SN  - 1522-2640 (eISSN)
N1  - ProcessNet-Jahrestagung und 33. DECHEMA-Jahrestagung der Biotechnologen 2018, 10. - 13. September 2018, Eurogress Aachen
VL  - 90
IS  - 9
SP  - 1154
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - GEN
A1  - Capitain, C. C.
A1  - Lukeba, L.
A1  - Ulber, Roland
A1  - Tippkötter, Nils
T1  - Biomimetische Klebstoffe aus Organosolv-Lignin
T2  - Chemie Ingenieur Technik
N2  - Aufgrund von EU-Regularien und Umweltinitiativen wächst der Markt für nachhaltige und abbaubare Klebstoffe stetig. Organosolv (OS)-Lignin ist ein kommerziell wenig ertragreicher Nebenstrom der Lignocellulose-Bioraffinerie. Durch das "Nachahmen" der Adhäsionseigenschaften mit strukturverwandten Muschel-Aminosäuren soll OS-Lignin in einen starkes, vollständig biobasiertes Adhäsiv umgewandelt werden. Funktionsweisend für die Adhäsion des Muschelklebstoffes ist die Catecholgruppe der Aminosäure L-DOPA. Die laccase-katalysierte Polymerisationsreaktion von Lignin und L-DOPA ist schwierig zu kontrollieren, da L-DOPA eine Ringschlussreaktion eingeht. Stattdessen wurde eine zweistufige Reaktion mit einem Diamin als Ankermolekül etabliert. Die Catecholgruppe, die im zweiten Schritt enzymatisch an das Lignin-Amin gebunden wird, kann durch Komplexbildung mit Fe(III)-Ionen sowohl zur Adhäsion als auch zur Kohäsion des Klebstoffes beitragen. Der Lignin-Catechol-Klebstoff ist frei von petrochemischen Chemikalien und biologisch abbaubar. In ersten Stirnzugversuchen konnte eine Haftkraft von 0,3 MPa erreicht werden.
Y1  - 2018
U6  - https://doi.org/10.1002/cite.201855076
SN  - 0009-286X
SN  - 1522-2640 (eISSN)
N1  - ProcessNet-Jahrestagung und 33. DECHEMA-Jahrestagung der Biotechnologen 2018, 10. - 13. September 2018, Eurogress Aachen
VL  - 90
IS  - 9
SP  - 1167
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - CHAP
A1  - Finkenberger, Isabel Maria
T1  - Die Stadt als städtisch-theatrale Versuchsanordnung: Wie wollen wir in Zukunft leben und welche Stadt brauchen wir dafür?
T2  - Un/Certain Futures - Rollen des Designs in gesellschaftlichen Transformationsprozessen
Y1  - 2024
SN  - 9783839443323
U6  - https://doi.org/https://doi.org/10.1515/9783839443323-009
SP  - 86
EP  - 95
PB  - transcript
CY  - Bielefeld
ER  - 
TY  - CHAP
A1  - Baader, Fabian
A1  - Reiswich, M.
A1  - Bartsch, M.
A1  - Keller, D.
A1  - Tiede, E.
A1  - Keck, G.
A1  - Demircian, A.
A1  - Friedrich, M.
A1  - Dachwald, Bernd
A1  - Schüller, K.
A1  - Lehmann, Raphael
A1  - Chojetzki, R.
A1  - Durand, C.
A1  - Rapp, L.
A1  - Kowalski, Julia
A1  - Förstner, R.
T1  - VIPER - Student research on extraterrestrical ice penetration technology
T2  - Proceedings of the 2nd Symposium on Space Educational Activities
N2  - Recent analysis of scientific data from Cassini and earth-based observations gave evidence for a global ocean under a surrounding solid ice shell on Saturn's moon Enceladus. Images of Enceladus' South Pole showed several fissures in the ice shell with plumes constantly exhausting frozen water particles, building up the E-Ring, one of the outer rings of Saturn. In this southern region of Enceladus, the ice shell is considered to be as thin as 2 km, about an order of magnitude thinner than on the rest of the moon. Under the ice shell, there is a global ocean consisting of liquid water. Scientists are discussing different approaches the possibilities of taking samples of water, i.e. by melting through the ice using a melting probe. FH Aachen UAS developed a prototype of maneuverable melting probe which can navigate through the ice that has already been tested successfully in a terrestrial environment. This means no atmosphere and or ambient pressure, low ice temperatures of around 100 to 150K (near the South Pole) and a very low gravity of 0,114 m/s^2 or 1100 μg. Two of these influencing measures are about to be investigated at FH Aachen UAS in 2017, low ice temperature and low ambient pressure below the triple point of water. Low gravity cannot be easily simulated inside a large experiment chamber, though. Numerical simulations of the melting process at RWTH Aachen however are showing a gravity dependence of melting behavior. Considering this aspect, VIPER provides a link between large-scale experimental simulations at FH Aachen UAS and numerical simulations at RWTH Aachen. To analyze the melting process, about 90 seconds of experiment time in reduced gravity and low ambient pressure is provided by the REXUS rocket. In this time frame, the melting speed and contact force between ice and probes are measured, as well as heating power and a two-dimensional array of ice temperatures. Additionally, visual and infrared cameras are used to observe the melting process.
Y1  - 2018
SP  - 1
EP  - 6
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Wehlitz, V.
A1  - Kayser, Peter
A1  - Figiel-Lange, A.
A1  - Bassam, Rasha
A1  - Rundstedt, F. von
T1  - Suspension depletion approach for exemption of infected Solanum jasminoides cells from pospiviroids
JF  - Plant Pathology
N2  - Despite numerous studies, viroid elimination from infected plants remains a very challenging task. This study introduces for the first time a novel ‘suspension depletion’ approach for exemption of Solanum jasminoides plants from viroids. The proposed method implies initial establishment of suspension cultures of the infected plant cells. The suspended cells were then physically treated (mild thermotherapy, 33 °C), which presumably delayed the replication of the viroid. The viroid concentration in the treated biomass was monitored weekly using pospiviroid-specific PCR. After 10–12 weeks of continuous treatment, a sufficient decrease in viroid concentration was observed such that the infection became undetectable by PCR. The treated single cells then gave rise to microcolonies on a solid culture medium and the obtained viroid-negative clones were further promoted to regenerate into viroid-free plants. Three years of accumulated experimental data suggests feasibility, broad applicability, and good efficacy of the proposed approach.
Y1  - 2018
U6  - https://doi.org/10.1111/ppa.12750
SN  - 1365-3059
VL  - 67
IS  - 2
SP  - 358
EP  - 365
PB  - Wiley
CY  - Oxford
ER  - 
TY  - CHAP
A1  - Koch, Claudia
A1  - Poghossian, Arshak
A1  - Wege, Christina
A1  - Schöning, Michael Josef
ED  - Wege, Christina
T1  - TMV-Based Adapter Templates for Enhanced Enzyme Loading in Biosensor Applications
T2  - Virus-Derived Nanoparticles for Advanced Technologies
N2  - Nanotubular tobacco mosaic virus (TMV) particles and RNA-free lower-order coat protein (CP) aggregates have been employed as enzyme carriers in different diagnostic layouts and compared for their influence on biosensor performance. In the following, we describe a label-free electrochemical biosensor for improved glucose detection by use of TMV adapters and the enzyme glucose oxidase (GOD). A specific and efficient immobilization of streptavidin-conjugated GOD ([SA]-GOD) complexes on biotinylated TMV nanotubes or CP aggregates was achieved via bioaffinity binding. Glucose sensors with adsorptively immobilized [SA]-GOD, and with [SA]-GOD cross-linked with glutardialdehyde, respectively, were tested in parallel on the same sensor chip. Comparison of these sensors revealed that TMV adapters enhanced the amperometric glucose detection remarkably, conveying highest sensitivity, an extended linear detection range and fastest response times. These results underline a great potential of an integration of virus/biomolecule hybrids with electronic transducers for applications in biosensorics and biochips. Here, we describe the fabrication and use of amperometric sensor chips combining an array of circular Pt electrodes, their loading with GOD-modified TMV nanotubes (and other GOD immobilization methods), and the subsequent investigations of the sensor performance.
KW  - Tobacco mosaic virus (TMV)
KW  - Coat protein
KW  - Enzyme nanocarrier
KW  - Glucose biosensor
KW  - Glucose oxidase
Y1  - 2018
SN  - 978-1-4939-7808-3
U6  - https://doi.org/10.1007/978-1-4939-7808-3
N1  - Methods in Molecular Biology, vol 1776
SP  - 553
EP  - 568
PB  - Humana Press
CY  - New York, NY
ER  - 
TY  - JOUR
A1  - Koch, Claudia
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
A1  - Wege, Christian
T1  - Penicillin Detection by Tobacco Mosaic Virus-Assisted Colorimetric Biosensors
JF  - Nanotheranostics
N2  - The presentation of enzymes on viral scaffolds has beneficial effects such as an increased enzyme loading and a prolonged reusability in comparison to conventional immobilization platforms. Here, we used modified tobacco mosaic virus (TMV) nanorods as enzyme carriers in penicillin G detection for the first time. Penicillinase enzymes were conjugated with streptavidin and coupled to TMV rods by use of a bifunctional biotin-linker. Penicillinase-decorated TMV particles were characterized extensively in halochromic dye-based biosensing. Acidometric analyte detection was performed with bromcresol purple as pH indicator and spectrophotometry. The TMV-assisted sensors exhibited increased enzyme loading and strongly improved reusability, and higher analysis rates compared to layouts without viral adapters. They extended the half-life of the sensors from 4 - 6 days to 5 weeks and thus allowed an at least 8-fold longer use of the sensors. Using a commercial budget-priced penicillinase preparation, a detection limit of 100 µM penicillin was obtained. Initial experiments also indicate that the system may be transferred to label-free detection layouts.
Y1  - 2018
U6  - https://doi.org/10.7150/ntno.22114
SN  - 2206-7418
VL  - 2
IS  - 2
SP  - 184
EP  - 196
PB  - Ivyspring
CY  - Sydney
ER  - 
TY  - CHAP
A1  - Grundmann, Jan Thimo
A1  - Bauer, Waldemar
A1  - Borchers, Kai
A1  - Dumont, Etienne
A1  - Grimm, Christian D.
A1  - Ho, Tra-Mi
A1  - Jahnke, Rico
A1  - Lange, Caroline
A1  - Maiwald, Volker
A1  - Mikulz, Eugen
A1  - Quantius, Dominik
A1  - Reershemius, Siebo
A1  - Renger, Thomas
A1  - Riemann, Johannes
A1  - Sasaki, Kaname
A1  - Seefeldt, Patric
A1  - Spietz, Peter
A1  - Spröwitz, Tom
A1  - Toth, Norbert
A1  - Wejmo, Elisabet
A1  - Biele, Jens
A1  - Krause, Christian
A1  - Cerotti, Matteo
A1  - Peloni, Alessandro
A1  - Dachwald, Bernd
T1  - Small Spacecraft Solar Sailing for Small Solar System Body Multiple Rendezvous and Landing
T2  - 2018 IEEE Aerospace Conference : 3-10 March 2018
Y1  - 2018
SN  - 978-1-5386-2014-4
ER  - 
TY  - CHAP
A1  - Grundmann, Jan Thimo
A1  - Bauer, Waldemar
A1  - Biele, Jens
A1  - Boden, Ralf
A1  - Ceriotti, Matteo
A1  - Cordero, Federico
A1  - Dachwald, Bernd
A1  - Dumont, Etienne
A1  - Grimm, Christian D.
A1  - Herčík, David
A1  - Ho, Tra-Mi
A1  - Jahnke, Rico
A1  - Koch, Aaron D
A1  - Koncz, Alexander
A1  - Krause, Christian
A1  - Lange, Caroline
A1  - Lichtenheldt, Roy
A1  - Maiwald, Volker
A1  - Mikschl, Tobias
A1  - Mikulz, Eugen
A1  - Montenegro, Sergio
A1  - Pelivan, Ivanka
A1  - Peloni, Alessandro
A1  - Quantius, Dominik
A1  - Reershemius, Siebo
A1  - Renger, Thomas
A1  - Riemann, Johannes
A1  - Ruffer, Michael
A1  - Sasaki, Kaname
A1  - Schmitz, Nicole
A1  - Seboldt, Wolfgang
A1  - Seefeldt, Patric
A1  - Spietz, Peter
A1  - Spröwitz, Tom
A1  - Sznajder, Maciej
A1  - Tardivel, Simon
A1  - Tóth, Norbert
A1  - Wejmo, Elisabet
A1  - Wolff, Friederike
A1  - Ziach, Christian
T1  - Small spacecraft based multiple near-earth asteroid rendezvous and landing with near-term solar sails and ‘Now-Term ‘technologies
T2  - 69 th International Astronautical Congress (IAC)
N2  - Physical interaction with small solar system bodies (SSSB) is the next step in planetary science, planetary in-situ resource utilization (ISRU), and planetary defense (PD). It requires a broader understanding of the surface properties of the target objects, with particular interest focused on those near Earth. Knowledge of composition, multi-scale surface structure, thermal response, and interior structure is required to design, validate and operate missions addressing these three fields. The current level of understanding is occasionally simplified into the phrase, ”If you’ve seen one asteroid, you’ve seen one asteroid”, meaning that the in-situ characterization of SSSBs has yet to cross the threshold towards a robust and stable scheme of classification. This would enable generic features in spacecraft design, particularly for ISRU and science missions. Currently, it is necessary to characterize any potential target object sufficiently by a dedicated pre-cursor mission to design the mission which then interacts with the object in a complex fashion. To open up strategic approaches, much broader in-depth characterization of potential target objects would be highly desirable. In SSSB science missions, MASCOT-like nano-landers and instrument carriers which integrate at the instrument level to their mothership have met interest. By its size, MASCOT is compatible with small interplanetary missions. The DLR-ESTEC Gossamer Roadmap Science Working Groups‘ studies identified Multiple Near-Earth asteroid (NEA) Rendezvous (MNR) as one of the space science missions only feasible with solar sail propulsion. The Solar Polar Orbiter (SPO) study showed the ability to access any inclination, theDisplaced-L1 (DL1) mission operates close to Earth, where objects of interest to PD and for ISRU reside. Other studies outline the unique capability of solar sails to provide access to all SSSB, at least within the orbit of Jupiter, and significant progress has been made to explore the performance envelope of near-term solar sails for MNR. However, it is difficult for
sailcraft to interact physically with a SSSB. We expand and extend the philosophy of the recently qualified DLR Gossamer solar sail deployment technology using efficient multiple sub-spacecraft integration to also include landers for one-way in-situ investigations and sample-return missions by synergetic integration and operation of sail and lander. The MASCOT design concept and its characteristic features have created an ideal counterpart for thisand has already been adapted to the needs of the AIM spacecraft, former part of the NASA-ESA AIDA missionDesigning the 69th International Astronautical Congress (IAC), Bremen, Germany, 1-5 October 2018. IAC-18-F1.2.3 Page 2 of 17 combined spacecraft for piggy-back launch accommodation enables low-cost massively parallel access to the NEA population.
KW  - multiple NEA rendezvous
KW  - solar sail
KW  - GOSSAMER-1
KW  - MASCOT
KW  - small spacecraft
Y1  - 2018
N1  - 69th International Astronautical Congress (IAC), Bremen, Germany, 1-5 October 2018.
https://www.bho-legal.com/1-5-october-2018-69th-international-astronautical-congress-2018-in-bremen-germany/
SP  - 1
EP  - 18
ER  - 
TY  - CHAP
A1  - Grundmann, Jan Thimo
A1  - Bauer, Waldemar
A1  - Biele, Jens
A1  - Boden, Ralf
A1  - Ceriotti, Matteo
A1  - Cordero, Federico
A1  - Dachwald, Bernd
A1  - Dumont, Etienne
A1  - Grimm, Christian
A1  - Herčík, David
A1  - Herique, Alain
A1  - Ho, Tra-Mi
A1  - Jahnke, Rico
A1  - Koch, Aaron
A1  - Kofman, Wlodek
A1  - Koncz, Alexander
A1  - Krause, Christian
A1  - Lange, Caroline
A1  - Lichtenheldt, Roy
A1  - Maiwald, Volker
A1  - Mikschl, Tobias
A1  - Mikulz, Eugen
A1  - Montenegro, Sergio
A1  - Pelivan, Ivanka
A1  - Peloni, Alessandro
A1  - Plettemeier, Dirk
A1  - Quantius, Dominik
A1  - Reershemius, Siebo
A1  - Renger, Thomas
A1  - Riemann, Johannes
A1  - Ruffer, Michael
A1  - Sasaki, Kaname
A1  - Schmitz, Nicole
A1  - Seboldt, Wolfgang
A1  - Seefeldt, Patric
A1  - Spietz, Peter
A1  - Spröwitz, Tom
A1  - Sznajder, Maciej
A1  - Tardivel, Simon
A1  - Toth, Norbert
A1  - Wejmo, Elisabet
A1  - Wolff, Friederike
A1  - Ziach, Christian
T1  - Efficient massively parallel prospection for ISRU by multiple near-earth asteroid rendezvous using near-term solar sails and'now-term'small spacecraft solutions
T2  - 2nd Asteroid Science Intersections with In-Space Mine Engineering – ASIME 2018
N2  - Physical interaction with small solar system bodies (SSSB) is key for in-situ resource utilization (ISRU). The design of mining missions requires good understanding of SSSB properties, including composition, surface and interior structure, and thermal environment. But as the saying goes "If you've seen one asteroid, you've seen one Asteroid": Although some patterns may begin to appear, a stable and reliable scheme of SSSB classification still has to be evolved. Identified commonalities would enable generic ISRU technology and spacecraft design approaches with a high degree of re-use. Strategic approaches require much broader in-depth characterization of the SSSB populations of interest to the ISRU community. The DLR-ESTEC GOSSAMER Roadmap Science Working Groups identified target-flexible Multiple Near-Earth asteroid (NEA) Rendezvous (MNR) as one of the missions only feasible with solar sail propulsion, showed the ability to access any inclination and a wide range of heliocentric distances as well as continuous operation close to Earth's orbit where low delta-v objects reside.
Y1  - 2018
N1  - 2nd Asteroid Science Intersections with In-Space Mine Engineering – ASIME 2018
16-17 April 2018, Belval, Luxembourg
SP  - 1
EP  - 33
ER  -