TY - JOUR A1 - Werkhausen, Amelie A1 - Albracht, Kirsten A1 - Cronin, Neil J A1 - Paulsen, Gøran A1 - Bojsen-Møller, Jens A1 - Seynnes, Olivier R T1 - Effect of training-induced changes in achilles tendon stiffness on muscle-tendon behavior during landing JF - Frontiers in physiology N2 - During rapid deceleration of the body, tendons buffer part of the elongation of the muscle-tendon unit (MTU), enabling safe energy dissipation via eccentric muscle contraction. Yet, the influence of changes in tendon stiffness within the physiological range upon these lengthening contractions is unknown. This study aimed to examine the effect of training-induced stiffening of the Achilles tendon on triceps surae muscle-tendon behavior during a landing task. Twenty-one male subjects were assigned to either a 10-week resistance-training program consisting of single-leg isometric plantarflexion (n = 11) or to a non-training control group (n = 10). Before and after the training period, plantarflexion force, peak Achilles tendon strain and stiffness were measured during isometric contractions, using a combination of dynamometry, ultrasound and kinematics data. Additionally, testing included a step-landing task, during which joint mechanics and lengths of gastrocnemius and soleus fascicles, Achilles tendon, and MTU were determined using synchronized ultrasound, kinematics and kinetics data collection. After training, plantarflexion strength and Achilles tendon stiffness increased (15 and 18%, respectively), and tendon strain during landing remained similar. Likewise, lengthening and negative work produced by the gastrocnemius MTU did not change detectably. However, in the training group, gastrocnemius fascicle length was offset (8%) to a longer length at touch down and, surprisingly, fascicle lengthening and velocity were reduced by 27 and 21%, respectively. These changes were not observed for soleus fascicles when accounting for variation in task execution between tests. These results indicate that a training-induced increase in tendon stiffness does not noticeably affect the buffering action of the tendon when the MTU is rapidly stretched. Reductions in gastrocnemius fascicle lengthening and lengthening velocity during landing occurred independently from tendon strain. Future studies are required to provide insight into the mechanisms underpinning these observations and their influence on energy dissipation. KW - achilles tendon KW - energy absorption KW - energy dissipation KW - mechanical buffer KW - stiffness Y1 - 2018 U6 - https://doi.org/10.3389/fphys.2018.00794 SN - 1664-042X IS - 9 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Haeger, Gerrit A1 - Jolmes, Tristan A1 - Oyen, Sven A1 - Jaeger, Karl-Erich A1 - Bongaerts, Johannes A1 - Schörken, Ulrich A1 - Siegert, Petra T1 - Novel recombinant aminoacylase from Paraburkholderia monticola capable of N-acyl-amino acid synthesis JF - Applied Microbiology and Biotechnology N2 - N-Acyl-amino acids can act as mild biobased surfactants, which are used, e.g., in baby shampoos. However, their chemical synthesis needs acyl chlorides and does not meet sustainability criteria. Thus, the identification of biocatalysts to develop greener synthesis routes is desirable. We describe a novel aminoacylase from Paraburkholderia monticola DSM 100849 (PmAcy) which was identified, cloned, and evaluated for its N-acyl-amino acid synthesis potential. Soluble protein was obtained by expression in lactose autoinduction medium and co-expression of molecular chaperones GroEL/S. Strep-tag affinity purification enriched the enzyme 16-fold and yielded 15 mg pure enzyme from 100 mL of culture. Biochemical characterization revealed that PmAcy possesses beneficial traits for industrial application like high temperature and pH-stability. A heat activation of PmAcy was observed upon incubation at temperatures up to 80 °C. Hydrolytic activity of PmAcy was detected with several N-acyl-amino acids as substrates and exhibited the highest conversion rate of 773 U/mg with N-lauroyl-L-alanine at 75 °C. The enzyme preferred long-chain acyl-amino-acids and displayed hardly any activity with acetyl-amino acids. PmAcy was also capable of N-acyl-amino acid synthesis with good conversion rates. The best synthesis results were obtained with the cationic L-amino acids L-arginine and L-lysine as well as with L-leucine and L-phenylalanine. Exemplarily, L-phenylalanine was acylated with fatty acids of chain lengths from C8 to C18 with conversion rates of up to 75%. N-lauroyl-L-phenylalanine was purified by precipitation, and the structure of the reaction product was verified by LC–MS and NMR. KW - Chaperone KW - Biocatalysis KW - Aminoacylase KW - Acylation KW - Acyl-amino acids KW - Biosurfactants Y1 - 2024 U6 - https://doi.org/10.1007/s00253-023-12868-8 SN - 1432-0614 N1 - Corresponding author: Petra Siegert IS - 108 PB - Springer CY - Berlin ER - TY - JOUR A1 - Yang, Peng-Fei A1 - Kriechbaumer, Andreas A1 - Albracht, Kirsten A1 - Sanno, Maximilian A1 - Ganse, Bergita A1 - Koy, Timmo A1 - Shang, Peng A1 - brüggemann, Gert-Peter A1 - Müller, Lars Peter A1 - Rittweger, Jörn T1 - A novel optical approach for assessing in vivo bone segment deformation and its application in muscle-bone relationship studies in humans JF - Journal of Orthopaedic Translation Y1 - 2014 U6 - https://doi.org/10.1016/j.jot.2014.07.078 SN - 2214-0328 SN - 2214-031X VL - 2 IS - 4 SP - 238 EP - 238 PB - Elsevier CY - Singapore ER - TY - INPR A1 - Ringers, Christa A1 - Bialonski, Stephan A1 - Solovev, Anton A1 - Hansen, Jan N. A1 - Ege, Mert A1 - Friedrich, Benjamin M. A1 - Jurisch-Yaksi, Nathalie T1 - Preprint: Local synchronization of cilia and tissue-scale cilia alignment are sufficient for global metachronal waves T2 - bioRxiv N2 - Motile cilia are hair-like cell extensions present in multiple organs of the body. How cilia coordinate their regular beat in multiciliated epithelia to move fluids remains insufficiently understood, particularly due to lack of rigorous quantification. We combine here experiments, novel analysis tools, and theory to address this knowledge gap. We investigate collective dynamics of cilia in the zebrafish nose, due to its conserved properties with other ciliated tissues and its superior accessibility for non-invasive imaging. We revealed that cilia are synchronized only locally and that the size of local synchronization domains increases with the viscosity of the surrounding medium. Despite the fact that synchronization is local only, we observed global patterns of traveling metachronal waves across the multiciliated epithelium. Intriguingly, these global wave direction patterns are conserved across individual fish, but different for left and right nose, unveiling a chiral asymmetry of metachronal coordination. To understand the implications of synchronization for fluid pumping, we used a computational model of a regular array of cilia. We found that local metachronal synchronization prevents steric collisions and improves fluid pumping in dense cilia carpets, but hardly affects the direction of fluid flow. In conclusion, we show that local synchronization together with tissue-scale cilia alignment are sufficient to generate metachronal wave patterns in multiciliated epithelia, which enhance their physiological function of fluid pumping. Y1 - 2021 U6 - https://doi.org/10.1101/2021.11.23.469646 N1 - Veröffentlicht in eLife 12:e77701 (https://doi.org/10.7554/eLife.77701). ER - TY - JOUR A1 - von Häfen, Hajo A1 - Krautwald, Clemens A1 - Stolle, Jacob A1 - Bung, Daniel Bernhard A1 - Goseberg, Nils T1 - Overland flow of broken solitary waves over a two-dimensional coastal plane JF - Coastal Engineering N2 - Landslides, rock falls or related subaerial and subaqueous mass slides can generate devastating impulse waves in adjacent waterbodies. Such waves can occur in lakes and fjords, or due to glacier calving in bays or at steep ocean coastlines. Infrastructure and residential houses along coastlines of those waterbodies are often situated on low elevation terrain, and are potentially at risk from inundation. Impulse waves, running up a uniform slope and generating an overland flow over an initially dry adjacent horizontal plane, represent a frequently found scenario, which needs to be better understood for disaster planning and mitigation. This study presents a novel set of large-scale flume test focusing on solitary waves propagating over a 1:14.5 slope and breaking onto a horizontal section. Examining the characteristics of overland flow, this study gives, for the first time, insight into the fundamental process of overland flow of a broken solitary wave: its shape and celerity, as well as its momentum when wave breaking has taken place beforehand. KW - Landslide tsunamis KW - Hazard assessment KW - Large scale tests KW - Overland flow KW - Solitary waves Y1 - 2022 U6 - https://doi.org/10.1016/j.coastaleng.2022.104125 SN - 1872-7379 VL - 175 IS - August PB - Elsevier CY - Amsterdam ER - TY - GEN T1 - Richtlinie zur Durchführung von Sonderveranstaltungen in den Gebäuden und Liegenschaften der FH Aachen (Veranstaltungsrichtlinie) : vom 20. November 2019 T3 - FH-Mitteilungen - 124a/2019 KW - Amtliche Mitteilung KW - Richtlinie KW - Veranstaltungsrichtlinie KW - Durchführung von Sonderveranstaltungen Y1 - 2019 ER - TY - GEN T1 - Richtlinie zur Durchführung von Sonderveranstaltungen in den Gebäuden und Liegenschaften der FH Aachen (Veranstaltungsrichtlinie) : vom 20. November 2019 Anlage 1 aktualisiert am 22. Januar 2020 T3 - FH-Mitteilungen - 124b/2019 KW - Amtliche Mitteilung KW - Richtlinie KW - Veranstaltungsrichtlinie KW - Durchführung von Sonderveranstaltungen KW - Aktualisierung Y1 - 2019 ER - TY - GEN T1 - Richtlinie für die Beschäftigung und Vergütung studentischer und wissenschaftlicher Hilfskräfte an der Fachhochschule Aachen : vom 20. November 2019 T3 - FH-Mitteilungen - 125a/2019 KW - Amtliche Mitteilung KW - Richtlinie KW - studentische Hilfskräfte KW - wissenschaftliche Hilfkräfte Y1 - 2019 ER - TY - GEN T1 - Richtlinie für die Beschäftigung und Vergütung studentischer und wissenschaftlicher Hilfskräfte an der Fachhochschule Aachen : vom 20. November 2019 aktualisiert am 24. November 2022 T3 - FH-Mitteilungen - 125b/2019 KW - Amtliche Mitteilung KW - Richtlinie KW - studentische Hilfskräfte KW - wissenschaftliche Hilfkräfte Y1 - 2019 ER - TY - GEN T1 - Gleichstellungsquoten der FH Aachen für die Jahre 2019 –2021 : vom 20. November 2019 T3 - FH-Mitteilungen - 126/2019 KW - Amtliche Mitteilung KW - Gleichstellungsquoten KW - 2019 KW - 2020 KW - 2021 Y1 - 2019 ER - TY - GEN T1 - Ordnung zur Aufhebung der Prüfungsordnungen für den Masterstudiengang „Nuclear Applications“ vom 13. Februar 2004 (FH-Mitteilung Nr. 5/2004) und vom 25. Februar 2010 (FH-Mitteilung Nr. 15/2010) in den Fassungen der Bekanntmachung der Änderungsordnungen vom 6. November 2012 (FH-Mitteilung Nr. 120/2012), 18. Juni 2014 (FH- Mitteilung Nr. 72/2014) und vom 15. Juni 2015 (FH-Mitteilung Nr. 28/2015) : vom 29. November 2019 T3 - FH-Mitteilungen - 127/2019 KW - Amtliche Mitteilung KW - Aufhebungsordnung KW - Prüfungsordnung KW - Master KW - Nuclear Applications Y1 - 2023 ER - TY - GEN T1 - 5. Ordnung zur Änderung der Prüfungsordnung für die Bachelorstudiengänge „Betriebswirtschaft Praxis Plus“ und „Betriebswirtschaft Praxis Plus (Teilzeit)“ mit dem Studienabschluss „Bachelor of Science“ am Fachbereich Wirtschaftswissenschaften der Fachhochschule Aachen (PO – BWL Praxis Plus) : vom 29. November 2019 T3 - FH-Mitteilungen - 128/2019 KW - Amtliche Mitteilung KW - Änderungsordnung KW - Prüfungsordnung KW - Bachelor KW - Betriebswirtschaft Praxis Plus Y1 - 2023 ER - TY - GEN T1 - Prüfungsordnung für die Bachelorstudiengänge „Betriebswirtschaft Praxis Plus“ und „Betriebswirtschaft Praxis Plus (Teilzeit)“ mit dem Studienabschluss „Bachelor of Science“ am Fachbereich Wirtschaftswissenschaften der Fachhochschule Aachen (PO-BWL Praxis Plus) : vom 14. Januar 2014 – FH-Mitteilung Nr. 2/2014 in der Fassung der Bekanntmachung der Änderungsordnung vom 29. November 2019 – FH-Mitteilung Nr. 128/2019 (Nichtamtliche lesbare Fassung | Studienbeginn ab WS 2019/20) T3 - FH-Mitteilungen - 129/2019 KW - Amtliche Mitteilung KW - Lesbare Fassung KW - Prüfungsordnung KW - Bachelor KW - Betriebswirtschaft Praxis Plus Y1 - 2023 ER - TY - GEN T1 - 2. Änderung der Richtlinie des Rektorats der FH Aachen für die Vergabe von Lehrveranstaltungsprämien : vom 13. Dezember 2019 T3 - FH-Mitteilungen - 130/2019 KW - Amtliche Mitteilung KW - Änderung KW - Richtlinie KW - Rektorat KW - Lehrveranstaltungsprämien Y1 - 2019 ER - TY - GEN T1 - Richtlinie des Rektorats der FH Aachen für die Vergabe von Lehrveranstaltungsprämien : vom 16. Juli 2012 – FH-Mitteilung Nr. 68/2012 in der Fassung der Bekanntmachung der Änderung vom 13. Dezember 2019 – FH-Mitteilung Nr. 130/2019 (Nichtamtliche lesbare Fassung) T3 - FH-Mitteilungen - 131/2019 KW - Amtliche Mitteilung KW - Lesbare Fassung KW - Richtlinie KW - Rektorat KW - Lehrveranstaltungsprämien Y1 - 2019 ER - TY - GEN T1 - Prüfungsordnung für den Masterstudiengang Nuclear Applications im Fachbereich Chemie und Biotechnologie an der Fachhochschule Aachen : vom 17. Dezember 2019 T3 - FH-Mitteilungen - 132/2019 KW - Amtliche Mitteilung KW - Prüfungsordnung KW - Master KW - Nuclear Applications KW - Chemie und Biotechnologie Y1 - 2019 ER - TY - GEN T1 - Ordnung zur Aufhebung der Prüfungsordnung für den Masterstudiengang „Information Systems Engineering“ vom 22. Januar 2010 (FH-Mitteilung Nr. 5/2010), zuletzt geändert durch Änderungsordnung vom 4. Februar 2013 (FH-Mitteilung Nr. 6/2013) : vom 20. Dezember 2019 T3 - FH-Mitteilungen - 133/2019 KW - Amtliche Mitteilung KW - Aufhebungsordnung KW - Prüfungsordnung KW - Master KW - Information Systems Engineering Y1 - 2019 ER - TY - GEN T1 - Ordnung zur Aufhebung der Prüfungsordnung für den Masterstudiengang „Elektrotechnik“ (zuvor „Nachrichtentechnik“ und „Elektrotechnik und Informationstechnik“) vom 22. Januar 2010 (FH-Mitteilung Nr. 7/2010), zuletzt geändert durch Änderungsordnung vom 25. April 2016 (FH-Mitteilung Nr. 31/2016) : vom 20. Dezember 2019 T3 - FH-Mitteilungen - 134/2019 KW - Amtliche Mitteilung KW - Aufhebungsordnung KW - Prüfungsordnung KW - Master KW - Elektrotechnik Y1 - 2019 ER - TY - GEN T1 - Ordnung zur Aufhebung der Prüfungsordnung für die Masterstudiengänge „Energiewirtschaft & Informatik“ (3 Semester) und „Energiewirtschaft & Informatik“ (4 Semester) vom 4. Mai 2016 (FH-Mitteilung Nr. 59/2016) Fachbereich Energietechnik Fachbereich Medizintechnik und Technomathematik an der Fachhochschule Aachen : vom 16. Januar 2020 T3 - FH-Mitteilungen - 1/2020 KW - Amtliche Mitteilung KW - Aufhebungsordnung KW - Prüfungsordnung KW - Master KW - Energiewirtschaft & Informatik Y1 - 2020 ER - TY - GEN T1 - Verwaltungs- und Benutzungsordnung des Solar-Instituts Jülich (SIJ) : vom 27. Januar 2020 T3 - FH-Mitteilungen - 2/2020 KW - Amtliche Mitteilung KW - Verwaltungs- und Benutzungsordnung KW - Solar-Institut Jülich KW - SIJ Y1 - 2020 ER -