TY - JOUR A1 - Rolka, David A1 - Poghossian, Arshak A1 - Schöning, Michael Josef T1 - Integration of a capacitive EIS sensor into a FIA system for pH and penicillin determination JF - Sensors. 4 (2004) Y1 - 2004 SN - 1424-8220 SP - 84 EP - 94 ER - TY - JOUR A1 - Poghossian, Arshak A1 - Schöning, Michael Josef T1 - Detecting Both Physical and (Bio-)Chemical Parameters by Means of ISFET Devices JF - Electroanalysis. 16 (2004), H. 22 Y1 - 2004 SN - 1040-0397 SP - 1863 EP - 1872 ER - TY - CHAP A1 - Koch, Claudia A1 - Poghossian, Arshak A1 - Wege, Christina A1 - Schöning, Michael Josef ED - Wege, Christina T1 - TMV-Based Adapter Templates for Enhanced Enzyme Loading in Biosensor Applications T2 - Virus-Derived Nanoparticles for Advanced Technologies N2 - Nanotubular tobacco mosaic virus (TMV) particles and RNA-free lower-order coat protein (CP) aggregates have been employed as enzyme carriers in different diagnostic layouts and compared for their influence on biosensor performance. In the following, we describe a label-free electrochemical biosensor for improved glucose detection by use of TMV adapters and the enzyme glucose oxidase (GOD). A specific and efficient immobilization of streptavidin-conjugated GOD ([SA]-GOD) complexes on biotinylated TMV nanotubes or CP aggregates was achieved via bioaffinity binding. Glucose sensors with adsorptively immobilized [SA]-GOD, and with [SA]-GOD cross-linked with glutardialdehyde, respectively, were tested in parallel on the same sensor chip. Comparison of these sensors revealed that TMV adapters enhanced the amperometric glucose detection remarkably, conveying highest sensitivity, an extended linear detection range and fastest response times. These results underline a great potential of an integration of virus/biomolecule hybrids with electronic transducers for applications in biosensorics and biochips. Here, we describe the fabrication and use of amperometric sensor chips combining an array of circular Pt electrodes, their loading with GOD-modified TMV nanotubes (and other GOD immobilization methods), and the subsequent investigations of the sensor performance. KW - Tobacco mosaic virus (TMV) KW - Coat protein KW - Enzyme nanocarrier KW - Glucose biosensor KW - Glucose oxidase Y1 - 2018 SN - 978-1-4939-7808-3 U6 - https://doi.org/10.1007/978-1-4939-7808-3 N1 - Methods in Molecular Biology, vol 1776 SP - 553 EP - 568 PB - Humana Press CY - New York, NY ER - TY - JOUR A1 - Siqueira, José R. Jr. A1 - Abouzar, Maryam H. A1 - Poghossian, Arshak A1 - Zucolotto, Valtencir A1 - Oliveira, Osvaldo N. Jr. A1 - Schöning, Michael Josef T1 - Penicillin biosensor based on a capacitive field-effect structure functionalized with a dendrimer/carbon nanotube multilayer JF - Biosensors and Bioelectronics. 25 (2009), H. 2 Y1 - 2009 SN - 0956-5663 SP - 497 EP - 501 ER - TY - JOUR A1 - Gamella, Maria A1 - Zakharchenko, Andrey A1 - Guz, Nataliia A1 - Masi, Madeline A1 - Minko, Sergiy A1 - Kolpashchikov, Dmitry M. A1 - Iken, Heiko A1 - Poghossian, Arshak A1 - Schöning, Michael Josef A1 - Katz, Evgeny T1 - DNA computing system activated by electrochemically triggered DNA realease from a polymer-brush-modified electrode array JF - Electroanalysis N2 - An array of four independently wired indium tin oxide (ITO) electrodes was used for electrochemically stimulated DNA release and activation of DNA-based Identity, AND and XOR logic gates. Single-stranded DNA molecules were loaded on the mixed poly(N,N-dimethylaminoethyl methacrylate) (PDMAEMA)/poly(methacrylic acid) (PMAA) brush covalently attached to the ITO electrodes. The DNA deposition was performed at pH 5.0 when the polymer brush is positively charged due to protonation of tertiary amino groups in PDMAEMA, thus resulting in electrostatic attraction of the negatively charged DNA. By applying electrolysis at −1.0 V(vs. Ag/AgCl reference) electrochemical oxygen reduction resulted in the consumption of hydrogen ions and local pH increase near the electrode surface. The process resulted in recharging the polymer brush to the negative state due to dissociation of carboxylic groups of PMAA, thus repulsing the negatively charged DNA and releasing it from the electrode surface. The DNA release was performed in various combinations from different electrodes in the array assembly. The released DNA operated as input signals for activation of the Boolean logic gates. The developed system represents a step forward in DNA computing, combining for the first time DNA chemical processes with electronic input signals. Y1 - 2017 U6 - https://doi.org/10.1002/elan.201600389 SN - 1521-4109 VL - 29 IS - 2 SP - 398 EP - 408 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Abouzar, Maryam H. A1 - Poghossian, Arshak A1 - Razavi, Arash A1 - Besmehn, Astrid A1 - Bijnens, Nathalie A1 - Williams, Oliver A. A1 - Haenen, Ken A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Penicillin detection with nanocrystalline-diamond field-effect sensor JF - physica status solidi (a). 205 (2008), H. 9 Y1 - 2008 SN - 1862-6319 N1 - Special Issue: Hasselt Diamond Workshop 2008 - SBDD XIII SP - 2141 EP - 2145 ER - TY - JOUR A1 - Abouzar, Maryam H. A1 - Ingebrandt, S. A1 - Poghossian, Arshak A1 - Zhang, Y. A1 - Moritz, W. A1 - Schöning, Michael Josef T1 - Field-effect nanoplate capacitive pH sensor based on SOI structure JF - Semiconductor micro- and nanoelectronics : Proceedings of the Seventh International Conference , Tsakhcadzor, Armenia July 3-5 2009 Y1 - 2009 SP - 55 EP - 58 ER - TY - JOUR A1 - Poghossian, Arshak A1 - Schöning, Michael Josef T1 - "High-order” hybrid FET module for (bio)chemical and physical sensing JF - Integrated analytical systems / ed. by Salvador Alegret Y1 - 2003 SN - 0-444-51037-0 SP - 587 EP - 623 PB - Elsevier CY - Amsterdam [u.a.] ER - TY - JOUR A1 - Wu, Chunsheng A1 - Bronder, Thomas A1 - Poghossian, Arshak A1 - Werner, Frederik A1 - Schöning, Michael Josef T1 - Label-free detection of DNA using light-addressable potentiometric sensor modified with a positively charged polyelectrolyte layer JF - Nanoscale N2 - A multi-spot (16 spots) light-addressable potentiometric sensor (MLAPS) consisting of an Al–p-Si–SiO2 structure modified with a weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)) was applied for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge for the first time. To achieve a preferentially flat orientation of DNA strands and thus, to reduce the distance between the DNA charge and MLAPS surface, the negatively charged probe single-stranded DNAs (ssDNA) were electrostatically adsorbed onto the positively charged PAH layer using a simple layer-by-layer (LbL) technique. In this way, more DNA charge can be positioned within the Debye length, yielding a higher sensor signal. The surface potential changes in each spot induced due to the surface modification steps (PAH adsorption, probe ssDNA immobilization, hybridization with complementary target DNA (cDNA), non-specific adsorption of mismatched ssDNA) were determined from the shifts of photocurrent–voltage curves along the voltage axis. A high sensor signal of 83 mV was registered after immobilization of probe ssDNA onto the PAH layer. The hybridization signal increases from 5 mV to 32 mV with increasing the concentration of cDNA from 0.1 nM to 5 μM. In contrast, a small signal of 5 mV was recorded in the case of non-specific adsorption of fully mismatched ssDNA (5 μM). The obtained results demonstrate the potential of the MLAPS in combination with the simple and rapid LbL immobilization technique as a promising platform for the future development of multi-spot light-addressable label-free DNA chips with direct electrical readout. Y1 - 2015 U6 - https://doi.org/10.1039/C4NR07225A VL - 14 IS - 7 SP - 6143 EP - 6150 PB - Royal Society of Chemistry (RSC) CY - Cambridge ER - TY - JOUR A1 - Beging, Stefan A1 - Mlynek, Daniela A1 - Hataihimakul, Sudkanung A1 - Poghossian, Arshak A1 - Baldsiefen, Gerhard A1 - Busch, Heinz A1 - Laube, Norbert A1 - Kleinen, Lisa A1 - Schöning, Michael Josef T1 - Field-effect calcium sensor for the determination of the risk of urinary stone formation JF - Sensors and Actuators B: Chemical. 144 (2010), H. 2 Y1 - 2010 N1 - 22nd International Conference on Eurosensors - Dresden, Germany, 7-10 September 2008 ; Eurosensors ; (22, 2008, Dresden) SP - 374 EP - 379 ER -