TY - JOUR A1 - Ribitsch, D. A1 - Heumann, S. A1 - Karl, W. A1 - Gerlach, J. A1 - Leber, R. A1 - Birner-Gruenberger, R. A1 - Gruber, K. A1 - Eiteljoerg, I. A1 - Remler, P. A1 - Siegert, Petra A1 - Lange, J. A1 - Maurer, Karl-Heinz A1 - Berg, G. A1 - Guebitz, G. M. A1 - Schwab, H. T1 - Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli JF - Journal of biotechnology N2 - A large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. Several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. Encapsulation of cells in alginate beads induced protease production. Stenotrophomonas maltophilia emerged as best performer under washing conditions. For identification of wash-active proteases, four extracellular serine proteases called StmPr1, StmPr2, StmPr3 and StmPr4 were cloned. StmPr2 and StmPr4 were sufficiently overexpressed in E. coli. Expression of StmPr1 and StmPr3 resulted in unprocessed, insoluble protein. Truncation of most of the C-terminal domain which has been identified by enzyme modeling succeeded in expression of soluble, active StmPr1 but failed in case of StmPr3. From laundry application tests StmPr2 turned out to be a highly wash-active protease at 45 °C. Specific activity of StmPr2 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 17 ± 2 U/mg. In addition we determined the kinetic parameters and cleavage preferences of protease StmPr2. KW - Alginate beads KW - Stenotrophomonas maltophilia KW - Detergent protease Y1 - 2012 U6 - https://doi.org/10.1016/j.jbiotec.2011.09.025 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 157 IS - 1 SP - 140 EP - 147 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Poghossian, Arshak A1 - Schusser, Sebastian A1 - Bäcker, Matthias A1 - Leinhos, Marcel A1 - Schöning, Michael Josef T1 - Real-time in-situ electrical monitoring of the degradation of biopolymers using semiconductor field-effect devices T2 - Biodegradable biopolymers. Vol. 1 Y1 - 2015 SN - 978-1-63483-632-6 SP - 135 EP - 153 PB - Nova Science Publ. CY - Hauppauge ER - TY - CHAP A1 - Beging, Stefan A1 - Poghossian, Arshak A1 - Mlynek, D. A1 - Hataihimakul, S. A1 - Pedraza, A. A1 - Dhawan, S. A1 - Laube, N. A1 - Kleinen, Lisa A1 - Baldsiefen, Gerhard A1 - Busch, Heinrich von A1 - Schöning, Michael Josef T1 - Ion-selective sensors for the determination of the risk of urinary stone formation T2 - Micro- and Nanosystems in biochemical diagnosis : Principles and applications Y1 - 2010 SP - 74 EP - 80 CY - Warsaw ER - TY - JOUR A1 - Beging, Stefan A1 - Poghossian, Arshak A1 - Schöning, Michael Josef A1 - Hataihimakul, Sudkanung A1 - Busch, Heinz A1 - Baldsiefen, Gerhard A1 - Laube, N. A1 - Kleinen, Lisa A1 - Hosseiny, Reyhaneh T1 - Feldeffektbasierender Ca2+-sensitiver Sensor für den Einsatz im Nativurin zur Bestimmung des Harnsteinbildungsrisikos JF - Sensoren und Messsysteme 2008 : 14. Fachtagung Ludwigsburg, 11. und 12. März 2008 / VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik Y1 - 2008 SN - 978-3-18-092011-5 N1 - VDI-Berichte ; 2011 ; Sensoren und Messsysteme 2008, 14. GMA/ITG-Fachtagung, VDI/VDE- Gesellschaft Mess- und Automatisierungstechnik, + CD-ROM, Ludwigsburg, DE, 11.-12. Mar, 2008 SP - 775 EP - 782 PB - VDI-Verl. CY - Düsseldorf ER - TY - JOUR A1 - Miyamoto, Ko-ichiro A1 - Kaneko, Kazumi A1 - Matsuo, Akira A1 - Wagner, Torsten A1 - Kanoh, Shin`ichiro A1 - Schöning, Michael Josef A1 - Yoshinobu, Tatsuo T1 - Miniaturized chemical imaging sensor system using an OLED display panel JF - Procedia Engineering N2 - The chemical imaging sensor is a semiconductor-based chemical sensor that can visualize the two-dimensional distribution of specific ions or molecules in the solution. In this study, we developed a miniaturized chemical imaging sensor system with an OLED display panel as a light source that scans the sensor plate. In the proposed configuration, the display panel is placed directly below the sensor plate and illuminates the back surface. The measured area defined by illumination can be arbitrarily customized to fit the size and the shape of the sample to be measured. The waveform of the generated photocurrent, the currentvoltage characteristics and the pH sensitivity were investigated and pH imaging with this miniaturized system was demonstrated. Y1 - 2010 U6 - https://doi.org/10.1016/j.proeng.2010.09.160 SN - 1877-7058 N1 - Eurosensor XXIV Conference, 5-8 September 2010, Linz, Austria VL - 5 SP - 516 EP - 519 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Poghossian, Arshak A1 - Wagner, Holger A1 - Schöning, Michael Josef T1 - Automatisiertes „wafer level“-Testsystem zur Charakterisierung von siliziumbasierten Chemo- und Biosensoren JF - Tagungsband: Sensoren und Messsysteme 2010 N2 - Es wurde ein automatisiertes, computerunterstütztes Testsystem für die Funktionsprüfung und Charakterisierung von (bio-)chemischen Sensoren auf Waferebene entwickelt und in einen konventionellen Spitzenmessplatz integriert. Das System ermöglicht die Charakterisierung und Identifizierung „funktionstauglicher“ Sensoren bereits auf Waferebene zwischen den einzelnen Herstellungsschritten, wodurch weitere, bisher übliche Verarbeitungsschritte wie das Fixieren, Bonden und Verkapseln für die defekten oder nicht funktionstauglichen Sensorstrukturen entfällt. Außerdem bietet eine speziell entworfene miniaturisierte Durchflussmesszelle die Möglichkeit, bereits auf Waferlevel die Sensitivität, Drift, Hysterese und Ansprechzeit der (bio-)chemischen Sensoren zu charakterisieren. Das System wurde exemplarisch mit kapazitiven, pH-sensitiven EIS- (Elektrolyt-Isolator-Silizium) Strukturen und ISFET- (ionensensitiver Feldeffekttransistor) Strukturen mit verschiedenen Geometrien und Gate-Layouts getestet. Y1 - 2010 SN - 978-3-8007-3260-9 N1 - Sensoren und Messsysteme 2010 - 15. ITG/GMA-Fachtagung, 18.05.2010 - 19.05.2010 in Nürnberg SP - 89 EP - 92 PB - VDE Verlag CY - Berlin ER - TY - JOUR A1 - Kirchner, Patrick A1 - Oberländer, Jan A1 - Friedrich, Peter A1 - Rysstad, Gunnar A1 - Berger, Jörg A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Realization of a calorimetric gas sensor on polyimide foil for applications in aseptic food industry JF - Procedia Engineering N2 - A calorimetric gas sensor is presented for the monitoring of gas-phase H2O2 at elevated temperature during sterilization processes in aseptic food industry. The sensor consists of two temperature-sensitive thin-film resistances built up on a polyimide foil with a thickness of 25 μm, which are passivated with a layer of SU-8 photo resist and catalytically activated with manganese(IV) oxide. Instead of an active heating structure, the calorimetric sensor utilizes the elevated temperature of an evaporated H2O2 aerosol. In an experimental set-up, the sensor has shown a sensitivity of 4.78 °C/(%v/v) in a H2O2 concentration range of 0 to 10% v/v at an evaporation temperature of 240 ∘C. Furthermore, the sensor possesses the same, unchanged sensor signal even at varied evaporation temperatures of the gas stream. The sensor characterization demonstrates the suitability of the calorimetric gas sensor for monitoring the efficiency of sterilization processes. Y1 - 2010 U6 - https://doi.org/10.1016/j.proeng.2010.09.098 SN - 1877-7058 N1 - Eurosensor XXIV Conference, 5-8 September 2010, Linz, Austria VL - 5 SP - 264 EP - 267 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Bohrn, Ulrich A1 - Stütz, Evamaria A1 - Fleischer, Maximilian A1 - Schöning, Michael Josef T1 - Real-time detection of CO by eukaryotic cells JF - Procedia Engineering N2 - In this contribution, we focus on the detection of toxic gases with living eukaryotic cells. A cell-based gas sensor system, able to measure the effects of direct exposure of gases to cells in real-time, was set up. Impedance data as well as oxygen consumption of Chinese hamster lung fibroblast cells (V79) were analysed upon exposure to carbon monoxide (CO). The CO (diluted in wet synthetic air) affects the cell respiration as indicated by an attenuated respiration signal after the CO exposure as well as an instant increase of the capacitive part of the impedance signal during the gas exposure. Y1 - 2010 SN - 1877-7058 N1 - Eurosensor XXIV Conference, 5-8 September 2010, Linz, Austria VL - 5 SP - 17 EP - 20 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Kirchner, Patrick A1 - Spelthahn, Heiko A1 - Schöning, Michael Josef A1 - Henkel, Hartmut A1 - Schneider, Andreas A1 - Friedrich, Peter A1 - Kolstad, Jens A1 - Berger, Jörg T1 - Realisierung eines Polyimid-basierten kalorimetrischen Gassensors zur Inline-Überwachung der H2O2-Konzentration in aseptischen Abfüllsystemen JF - Tagungsband: Sensoren und Messsysteme 2010 N2 - In aseptischen Abfüllsystemen wird Wasserstoffperoxid in der Gasphase aufgrund der stark oxidativen Wirkung zur Packstoffentkeimung eingesetzt. Dabei wird die Effizienz der Entkeimung im Wesentlichen von der vorliegenden H2O2-Konzentration im Packstoff bestimmt. Zur Inline-Überwachung der H2O2-Konzentration wurde ein kalorimetrischer Gassensor auf Basis einer flexiblen Polyimidfolie aus temperatursensitiven Dünnschicht-Widerständen und Mangan(IV)-oxid als katalytische Transducerschicht realisiert. Der Sensor weist ein lineares Ansprechverhalten mit einer Sensitivität von 7,15 °C/Vol.-% in einem H2O2-Konzentrationsbereich von 0 bis 8 Vol.-% auf. Weiterhin wurde zur Auslesung des Sensorsignals eine RFID-Elektronik, bestehend aus einem Sensor-Tag und einer Sende-/Empfangseinheit ausgelegt, sowie eine Abfolge des Messzyklus aufgestellt. Im weiteren Verlauf soll der kalorimetrische Gassensor mit der RFID-Elektronik gekoppelt und in eine Testverpackung zur Inline-Überwachung der H2O2-Konzentration in aseptischen Abfüllsystemen implementiert werden. Y1 - 2010 SN - 978-3-8007-3260-9 N1 - Sensoren und Messsysteme 2010 - 15. ITG/GMA-Fachtagung, 18.05.2010 - 19.05.2010 in Nürnberg SP - 607 EP - 612 PB - VDE Verlag CY - Berlin ER - TY - JOUR A1 - Spelthahn, Heiko A1 - Schaffrath, Sophie A1 - Coppe, Thomas A1 - Rufi, Frederic A1 - Schöning, Michael Josef T1 - Development of an electrolyte-insulator-semiconductor (EIS) based capacitive heavy metal sensor for the detection of Pb2+ und Cd2+ ions JF - Physica status solidi (a) : applications and material science N2 - Chalcogenide glass materials as membranes for potentiometric sensors for chemical analysis in solutions have been studied since more than 20 years. In this work, an electrolyte–insulator–semiconductor structure was combined with chalcogenide glass membranes prepared by means of the pulsed laser deposition technique. Depending on the membrane composition a selectivity to different ions (Cd2+ and Pb2+) is achieved. The different sensor membranes have been physically characterised using microscopy, ellipsometry, profilometry, atomic force microscopy (AFM), scanning electron microscopy (SEM) and Rutherford backscattering spectrometry (RBS). The electrochemical behaviour has been investigated via capacitance/voltage (C/V) and constant capacitance (ConCap) measurements and results in a Cd2+ sensitivity of 23.1 ± 0.6 mV per decade in a linear range from 7 × 10−6 to 10−2 mol/l and 24.4 ± 0.5 mV per decade in a linear range from 5 × 10−6 to 10−2 mol/l for Pb2+, respectively. Y1 - 2010 U6 - https://doi.org/10.1002/pssa.200983306 SN - 1862-6300 N1 - Special Issue: Engineering of Functional Interfaces EnFI 2009 VL - 207 IS - 4 SP - 930 EP - 934 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Werner, Frederik A1 - Spelthahn, Heiko A1 - Schöning, Michael Josef A1 - Krumbe, Christoph A1 - Wagner, Torsten A1 - Yoshinobu, Tatsuo A1 - Keusgen, Michael T1 - Neue Ansteuerungselektronik für LAPS-basierte Biosensoren zur gleichzeitig ortsaufgelösten Messung der pH-Konzentration JF - Tagungsband: Sensoren und Messsysteme 2010 N2 - Ein lichtadressierbarer potentiometrischer Sensor (LAPS) kann die Konzentration eines oder mehrerer Analyten ortsaufgelöst auf der Sensoroberfläche nachweisen. Dazu wird mit einer modulierten Lichtquelle die Halbleiterstruktur des zu untersuchenden Bereiches angeregt und ein entsprechender Photostrom ausgelesen. Durch gleichzeitige Anregung mehrere Bereiche durch Lichtquellen mit unterschiedlichen Modulationsfrequenzen können diese auch zeitgleich ausgelesen werden. Mit der neuen, hier vorgestellten Ansteuerungselektronik integriert in einem "Field Programmable Gate Array" (FPGA) ist es möglich, mehrere Leuchtquellen gleichzeitig mit unterschiedlichen, während der Laufzeit festlegbaren Frequenzen, Phasen und Lichtintensitäten zu betreiben. Somit kann das Frequenzverhalten des Sensors untersucht und die Konzentration des Analyten über das Oberflächenpotential mit Hilfe von Strom/Spannungs-Kurven und Phase/Spannungs-Kurven bestimmt werden. Y1 - 2010 SN - 978-3-8007-3260-9 N1 - Sensoren und Messsysteme 2010 - 15. ITG/GMA-Fachtagung, 18.05.2010 - 19.05.2010 in Nürnberg SP - 109 EP - 114 PB - VDE Verlag CY - Berlin ER - TY - JOUR A1 - Wagner, Torsten A1 - Miyamoto, Ko-ichiro A1 - Schöning, Michael Josef A1 - Yoshinobu, Tatsuo T1 - Novel combination of digital light processing (DLP) and light-addressable potentiometric sensors (LAPS) for flexible chemical imaging JF - Procedia Engineering N2 - Chemical imaging systems allow the visualisation of the distribution of chemical species on the sensor surface. This work represents a new flexible approach of read out in a light-addressable potentiometric sensor (LAPS) with the help of a digital light processing (DLP) set-up. The DLP, known well for video projectors, consists of a mirror-array MEMS device which allows fast and flexible generation of light patterns. With the help of these light patterns the sensor surface of the LAPS device can be read out sequentially in a raster like scheme (scanning LAPS). The DLP approach has several advantages compared to conventional scanning LAPS set-ups, e.g., the spot size, the shape and the intensity of the light pointer can be changed easily and no mechanical movement is necessary, which reduces the size of the set-up and increases the stability and speed of measurement. Y1 - 2010 U6 - https://doi.org/10.1016/j.proeng.2010.09.161 SN - 1877-7058 N1 - Eurosensor XXIV Conference, 5-8 September 2010, Linz, Austria VL - 5 SP - 520 EP - 523 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Abouzar, Maryam H. A1 - Pedraza, A. M. A1 - Schöning, Michael Josef A1 - Poghossian, Arshak T1 - Label-free DNA hybridization and denaturation detection by means of field-effect nanoplate SOI capacitors functionalized with gold nanoparticles JF - Procedia Engineering N2 - A new approach for a label-free electrical detection of DNA hybridization and denaturation using an array of individually addressable field-effect nanoplate SOI (silicon-on-insulator) capacitors functionalized with gold nanoparticles is presented. By using a constant-capacitance measuring setup in a differential mode, signal changes of ∼110 mV and ∼70 mV have been registered after the DNA hybridization and denaturation events, respectively. Y1 - 2010 U6 - https://doi.org/10.1016/j.proeng.2010.09.259 SN - 1877-7058 N1 - Eurosensor XXIV Conference, 5-8 September 2010, Linz, Austria VL - 5 SP - 918 EP - 921 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Wagner, Torsten A1 - Werner, Frederik A1 - Miyamoto, Ko-ichiro A1 - Ackermann, Hans-Josef A1 - Yoshinobu, Tatsuo A1 - Schöning, Michael Josef T1 - FPGA-based LAPS device for the flexible design of sensing sites on functional interfaces JF - Physica Status Solidi (A) N2 - The development of new interfaces for (bio-)chemical sensors requires comprehensive analyses and testing. The light-addressable potentiometric sensor (LAPS) can be used as a platform to investigate the sensitivity of a newly developed interface towards (bio-)chemical agents. LAPS measurements are spatially resolved by utilisation of focused light beams to define individual measurement spots. In this work, a new digitally modulated LAPS set-up based on an FPGA design will be introduced to increase the number of measurement spots, to shorten the measurement time and to improve the measurement accuracy. Y1 - 2010 U6 - https://doi.org/10.1002/pssa.200983320 SN - 1862-6300 N1 - Special Issue: Engineering of Functional Interfaces EnFI 2009 VL - 207 IS - 4 SP - 844 EP - 849 PB - Wiley-VCH CY - Weinheim ER -