TY - JOUR A1 - Bertz, Morten A1 - Molinnus, Denise A1 - Schöning, Michael Josef A1 - Homma, Takayuki T1 - Real-time monitoring of H₂O₂ sterilization on individual bacillus atrophaeus spores by optical sensing with trapping Raman spectroscopy JF - Chemosensors N2 - Hydrogen peroxide (H₂O₂), a strong oxidizer, is a commonly used sterilization agent employed during aseptic food processing and medical applications. To assess the sterilization efficiency with H₂O₂, bacterial spores are common microbial systems due to their remarkable robustness against a wide variety of decontamination strategies. Despite their widespread use, there is, however, only little information about the detailed time-resolved mechanism underlying the oxidative spore death by H₂O₂. In this work, we investigate chemical and morphological changes of individual Bacillus atrophaeus spores undergoing oxidative damage using optical sensing with trapping Raman microscopy in real-time. The time-resolved experiments reveal that spore death involves two distinct phases: (i) an initial phase dominated by the fast release of dipicolinic acid (DPA), a major spore biomarker, which indicates the rupture of the spore’s core; and (ii) the oxidation of the remaining spore material resulting in the subsequent fragmentation of the spores’ coat. Simultaneous observation of the spore morphology by optical microscopy corroborates these mechanisms. The dependence of the onset of DPA release and the time constant of spore fragmentation on H₂O₂ shows that the formation of reactive oxygen species from H₂O₂ is the rate-limiting factor of oxidative spore death. KW - DPA (dipicolinic acid) KW - sterilization KW - Bacillus atrophaeus spores KW - optical trapping KW - Raman spectroscopy KW - optical sensor setup Y1 - 2023 U6 - https://doi.org/10.3390/chemosensors11080445 SN - 2227-9040 N1 - This article belongs to the Special Issue "Biosensors and Chemical Sensors for Food and Healthcare Monitoring—Celebrating the 10th Anniversary" VL - 8 IS - 11 PB - MDPI CY - Basel ER - TY - BOOK A1 - Czarnecki, Christian ED - Czarnecki, Christian ED - Lübbe, Alexander ED - Meister, Vera G. ED - Müller, Christian ED - Steglich, Mike ED - Walther, Mathias T1 - Angewandte Forschung in der Wirtschaftsinformatik 2023 : Tagungsband zur 36. AKWI-Jahrestagung N2 - Beiträge der Fachtagung „Angewandte Forschung in der Wirtschaftsinformatik – Zukunft gestalten“ im Rahmen der 36. Jahrestagung des Arbeitskreises Wirtschaftsinformatik an Hochschulen für Angewandte Wissenschaften (AKWI) vom 11. bis 13.09.2023 an der Technischen Hochschule Wildau Y1 - 2023 U6 - https://doi.org/10.15771/1794 N1 - 36. AKWI-Jahrestagung, 11.09.2023 bis 13.09.2023, ausgerichtet von der Technischen Hochschule Wildau ER - TY - JOUR A1 - Golland, Alexander A1 - Kriegesmann, Torben T1 - Zivilprozessuale Fragen zum datenschutzrechtlichen Schadensersatzanspruch JF - MMR - Zeitschrift für IT-Recht und Recht der Digitalisierung N2 - Im Verfahren gegen die Österreichische Post AG (Rs. C-300/21) befasste sich der EuGH erstmals mit dem in Art. 82 DS-GVO geregelten datenschutzrechtlichen Schadensersatzanspruch. Mit den Klarstellungen des EuGH verschieben sich die Probleme nun stärker zu den „klassischen“ Fragen des Schadensersatzrechts im Zivilprozess. Relevant sind dabei vor allem Aspekte der Darlegungs- und Beweislast und deren Besonderheiten mit Blick auf den Ersatz immaterieller Schäden. Der Beitrag fokussiert sich auf die Voraussetzungen und den dabei zu führenden Tatsachenbeweis bei der Klage des Betroffenen gegen den Verantwortlichen auf Ersatz immaterieller Schäden. Y1 - 2023 SN - 2698-7988 IS - 10 SP - 733 EP - 739 PB - Beck CY - München ER - TY - GEN A1 - Steuer-Dankert, Linda A1 - Bernhard, Sebastian A1 - Langolf, Jessica A1 - Leicht-Scholten, Carmen T1 - About the paradox of sustainable production and what we can do about it! T2 - Joint SCORAI-ERSCP-WUR conference on transforming consumption-production systems toward just and sustainable futures (SCP23) N2 - Sustainability is playing an increasingly important role. Not least due to the definition of the sustainable development goals (SDGs) in the framework of the agenda 2030 by the United Nations (UN) in 2015 (United Nations, n.d.), it has become clear that the cooperation of different actors is needed to achieve the defined 17 goals. Industry, as a global actor, has a special role to play in this. In the course of sustainable production processes and chains, the industry is confronted with the responsibility of reflecting on the consequences of its own trade on an ecological, economic, and also social level and deriving measures that, according to the definition of sustainability (Hauff, 1987), will also enable future generations to satisfy their needs. While the ecological pillar of sustainability is already being addressed by different industrial initiatives (Deloitte, 2021), it is questionable to what extent the economic and, above all, the social pillars of sustainability also play a decisive role. Accordingly, it is questionable to what extent sustainability in its triad of social, ecological, and economic aspects is taken into account holistically at all, and thus to what extent the industry contributes to achieving the 17 goals defined by the UN. This paper presents a qualitative study that explores these questions. Interviewing 31 representatives from the manufacturing industry in Germany, results indicate a Paradox of Sustainable Production expressed by a theoretical reflection of the need for focusing on people in production processes on the one hand and a lack of addressing the social pillar of sustainability in concepts on the other hand. However, while it is a troublesome finding given the striking need for sustainable development (The-Sustainable-Development-Goals-Report-2022; Kropp 2019; von Hauff 2021; Roy and Singh 2017), the paradox directly lays out a path of resolving it. This is because, given its nature, we can see that we could resolve it via the implementation of strong educational efforts trying to help the respective people of the manufacturing industry to understand the holistic and interdependent character of sustainable development (The-Sustainable-Development-Goals-Report-2022). Y1 - 2023 N1 - Volltext auf dem Opus-Server verfügbar ER - TY - JOUR A1 - Baader, Fabian A1 - Boxberg, Marc S. A1 - Chen, Qian A1 - Förstner, Roger A1 - Kowalski, Julia A1 - Dachwald, Bernd T1 - Field-test performance of an ice-melting probe in a terrestrial analogue environment JF - Icarus N2 - Melting probes are a proven tool for the exploration of thick ice layers and clean sampling of subglacial water on Earth. Their compact size and ease of operation also make them a key technology for the future exploration of icy moons in our Solar System, most prominently Europa and Enceladus. For both mission planning and hardware engineering, metrics such as efficiency and expected performance in terms of achievable speed, power requirements, and necessary heating power have to be known. Theoretical studies aim at describing thermal losses on the one hand, while laboratory experiments and field tests allow an empirical investigation of the true performance on the other hand. To investigate the practical value of a performance model for the operational performance in extraterrestrial environments, we first contrast measured data from terrestrial field tests on temperate and polythermal glaciers with results from basic heat loss models and a melt trajectory model. For this purpose, we propose conventions for the determination of two different efficiencies that can be applied to both measured data and models. One definition of efficiency is related to the melting head only, while the other definition considers the melting probe as a whole. We also present methods to combine several sources of heat loss for probes with a circular cross-section, and to translate the geometry of probes with a non-circular cross-section to analyse them in the same way. The models were selected in a way that minimizes the need to make assumptions about unknown parameters of the probe or the ice environment. The results indicate that currently used models do not yet reliably reproduce the performance of a probe under realistic conditions. Melting velocities and efficiencies are constantly overestimated by 15 to 50 % in the models, but qualitatively agree with the field test data. Hence, losses are observed, that are not yet covered and quantified by the available loss models. We find that the deviation increases with decreasing ice temperature. We suspect that this mismatch is mainly due to the too restrictive idealization of the probe model and the fact that the probe was not operated in an efficiency-optimized manner during the field tests. With respect to space mission engineering, we find that performance and efficiency models must be used with caution in unknown ice environments, as various ice parameters have a significant effect on the melting process. Some of these are difficult to estimate from afar. Y1 - 2023 U6 - https://doi.org/10.1016/j.icarus.2023.115852 N1 - Forschungsdaten hierzu: "Performance data of an ice-melting probe from field tests in two different ice environments" (https://opus.bibliothek.fh-aachen.de/opus4/frontdoor/index/index/docId/10890) IS - 409 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Haeger, Gerrit A1 - Probst, Johanna A1 - Jaeger, Karl-Erich A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - Novel aminoacylases from Streptomyces griseus DSM 40236 and their recombinant production in Streptomyces lividans JF - FEBS Open Bio N2 - Amino acid-based surfactants are valuable compounds for cosmetic formulations. The chemical synthesis of acyl-amino acids is conventionally performed by the Schotten-Baumann reaction using fatty acyl chlorides, but aminoacylases have also been investigated for use in biocatalytic synthesis with free fatty acids. Aminoacylases and their properties are diverse; they belong to different peptidase families and show differences in substrate specificity and biocatalytic potential. Bacterial aminoacylases capable of synthesis have been isolated from Burkholderia, Mycolicibacterium, and Streptomyces. Although several proteases and peptidases from S. griseus have been described, no aminoacylases from this species have been identified yet. In this study, we investigated two novel enzymes produced by S. griseus DSM 40236ᵀ . We identified and cloned the respective genes and recombinantly expressed an α-aminoacylase (EC 3.5.1.14), designated SgAA, and an ε-lysine acylase (EC 3.5.1.17), designated SgELA, in S. lividans TK23. The purified aminoacylase SgAA was biochemically characterized, focusing on its hydrolytic activity to determine temperature- and pH optima and stabilities. The aminoacylase could hydrolyze various acetyl-amino acids at the Nα -position with a broad specificity regarding the sidechain. Substrates with longer acyl chains, like lauroyl-amino acids, were hydrolyzed to a lesser extent. Purified aminoacylase SgELA specific for the hydrolysis of Nε -acetyl-L-lysine was unstable and lost its enzymatic activity upon storage for a longer period but could initially be characterized. The pH optimum of SgELA was pH 8.0. While synthesis of acyl-amino acids was not observed with SgELA, SgAA catalyzed the synthesis of lauroyl-methionine. KW - Streptomyces lividans KW - recombinant expression KW - Streptomyces griseus KW - ε-lysine acylase KW - α-aminoacylase Y1 - 2023 U6 - https://doi.org/10.1002/2211-5463.13723 SN - 2211-5463 N1 - Corresponding author: Petra Siegert VL - 13 IS - 12 SP - 2224 EP - 2238 PB - Wiley CY - Hoboken, NJ ER - TY - JOUR A1 - Mues genannt Koers, Lucas A1 - McNeil, S. W. A1 - Radchenko, V. A1 - Paulßen, Elisabeth A1 - Hoehr, Cornelia T1 - Production of Co-58m in a siphon-style liquid target on a medical cyclotron N2 - We present the production of 58mCo on a small, 13 MeV medical cyclotron utilizing a siphon style liquid target system. Different concentrated iron(III)-nitrate solutions of natural isotopic distribution were irradiated at varying initial pressures and subsequently separated by solid phase extraction chromatography. The radio cobalt (58m/gCo and 56Co) was successfully produced with saturation activities of (0.35 ± 0.03) MBq μA−1 for 58mCo with a separation recovery of (75 ± 2) % of cobalt after one separation step utilizing LN-resin. Y1 - 2023 U6 - https://doi.org/10.1016/j.apradiso.2023.110734 SN - 0969-8043 VL - 195 IS - Art. 110734 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Treuling, Clemens A1 - Czarnecki, Christian A1 - Wolf, Martin R. T1 - Projekt WiLMo – Wirtschaftsinformatik Lehr- und Lernmodule T2 - Angewandte Forschung in der Wirtschaftsinformatik 2023 : Tagungsband zur 36. AKWI-Jahrestagung N2 - Die potenziellen Auswirkungen der Digitalisierung auf die Lehre sind seit langem Gegenstand ausführlicher Diskussionen innerhalb der Wirtschaftsinformatik (WI) (z. B. in Auth et al. 2021, Barton et al. 2019, Klotz et al. 2019). Nicht zuletzt der in nahezu allen Wirtschaftszweigen bestehende Mangel an qualifizierten Fachkräften lenkt den Diskurs auf einen verbesserten Zugang zu Bildung und gleichen Bildungschancen. Aus dieser Vision heraus und dem Schub der Digitalisierung entstehen Bildungskonzepte wie Open Educational Resources (OER), die gesellschaftlichen Problemen, wie dem des Fachkräftemangels, entgegenwirken sollen. Im Rahmen dieses Kurzbeitrags wird das Projekt WiLMo - "Wirtschaftsinformatik Lehr- und Lernmodule" vorgestellt. WiLMo wird im Rahmen von OERContent.nrw unter Beteiligung von sechs Hochschulen entwickelt und gefördert. Alle Projektbeteiligten arbeiten gemeinsam daran, einheitliche digitale Lehr- und Lernmaterialien im OER-Format für die Kernmodule der Wirtschaftsinformatik zu entwickeln und in garantiert hoher Qualität zur Verfügung zu stellen. KW - Wirtschaftsinformatik KW - Open Educational Resources (OER) KW - WiLMo Y1 - 2023 N1 - 36. AKWI-Jahrestagung, 11.09.2023 bis 13.09.2023, ausgerichtet von der Technischen Hochschule Wildau SP - 161 EP - 169 ER - TY - JOUR A1 - Wiegner, Jonas A1 - Volker, Hanno A1 - Mainz, Fabian A1 - Backes, Andreas A1 - Loeken, Michael A1 - Hüning, Felix T1 - Energy analysis of a wireless sensor node powered by a Wiegand sensor JF - Journal of Sensors and Sensor Systems (JSSS) N2 - This article describes an Internet of things (IoT) sensing device with a wireless interface which is powered by the energy-harvesting method of the Wiegand effect. The Wiegand effect, in contrast to continuous sources like photovoltaic or thermal harvesters, provides small amounts of energy discontinuously in pulsed mode. To enable an energy-self-sufficient operation of the sensing device with this pulsed energy source, the output energy of the Wiegand generator is maximized. This energy is used to power up the system and to acquire and process data like position, temperature or other resistively measurable quantities as well as transmit these data via an ultra-low-power ultra-wideband (UWB) data transmitter. A proof-of-concept system was built to prove the feasibility of the approach. The energy consumption of the system during start-up was analysed, traced back in detail to the individual components, compared to the generated energy and processed to identify further optimization options. Based on the proof of concept, an application prototype was developed. Y1 - 2023 U6 - https://doi.org/10.5194/jsss-12-85-2023 SN - 2194-878X N1 - Corresponding author: Felix Hüning VL - 12 IS - 1 SP - 85 EP - 92 PB - Copernicus Publ. CY - Göttingen ER - TY - CHAP A1 - Baier, Ralph A1 - Brauner, Philipp A1 - Brillowski, Florian A1 - Dammers, Hannah A1 - Liehner, Luca A1 - Pütz, Sebastian A1 - Schneider, Sebastian A1 - Schollemann, Alexander A1 - Steuer-Dankert, Linda A1 - Vervier, Luisa A1 - Gries, Thomas A1 - Leicht-Scholten, Carmen A1 - Mertens, Alexander A1 - Nagel, Saskia K. A1 - Schuh, Günther A1 - Ziefle, Martina A1 - Nitsch, Verena ED - Brecher, Christian ED - Schuh, Günther ED - van der Alst, Wil ED - Jarke, Matthias ED - Piller, Frank T. ED - Padberg, Melanie T1 - Human-centered work design for the internet of production T2 - Internet of production - fundamentals, applications and proceedings N2 - Like all preceding transformations of the manufacturing industry, the large-scale usage of production data will reshape the role of humans within the sociotechnical production ecosystem. To ensure that this transformation creates work systems in which employees are empowered, productive, healthy, and motivated, the transformation must be guided by principles of and research on human-centered work design. Specifically, measures must be taken at all levels of work design, ranging from (1) the work tasks to (2) the working conditions to (3) the organizational level and (4) the supra-organizational level. We present selected research across all four levels that showcase the opportunities and requirements that surface when striving for human-centered work design for the Internet of Production (IoP). (1) On the work task level, we illustrate the user-centered design of human-robot collaboration (HRC) and process planning in the composite industry as well as user-centered design factors for cognitive assistance systems. (2) On the working conditions level, we present a newly developed framework for the classification of HRC workplaces. (3) Moving to the organizational level, we show how corporate data can be used to facilitate best practice sharing in production networks, and we discuss the implications of the IoP for new leadership models. Finally, (4) on the supra-organizational level, we examine overarching ethical dimensions, investigating, e.g., how the new work contexts affect our understanding of responsibility and normative values such as autonomy and privacy. Overall, these interdisciplinary research perspectives highlight the importance and necessary scope of considering the human factor in the IoP. KW - Responsibility KW - Privacy KW - Digital leadership KW - Best practice sharing KW - Cognitive assistance system KW - Human-robot collaboration KW - Human-centered work design Y1 - 2023 SN - 978-3-030-98062-7 U6 - https://doi.org/10.1007/978-3-030-98062-7_19-1 N1 - Part of the book series: Interdisciplinary Excellence Accelerator Series (IDEAS) SP - 1 EP - 23 PB - Springer CY - Cham ER -